CN110959038A - 酶产物 - Google Patents

酶产物 Download PDF

Info

Publication number
CN110959038A
CN110959038A CN201880032258.6A CN201880032258A CN110959038A CN 110959038 A CN110959038 A CN 110959038A CN 201880032258 A CN201880032258 A CN 201880032258A CN 110959038 A CN110959038 A CN 110959038A
Authority
CN
China
Prior art keywords
enzyme
recombinase
product
cas registry
composition
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201880032258.6A
Other languages
English (en)
Chinese (zh)
Inventor
斯蒂芬·斯科纳特
马赛厄斯·撒拉莫
托马斯·斯科利辰
安德烈斯·瓦戈
塞布丽娜·库扑克
塞巴斯蒂安·巴斯
波吉特·布鲁赫
克劳迪亚·芬勒
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
C Lecta GmbH
Original Assignee
C Lecta GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by C Lecta GmbH filed Critical C Lecta GmbH
Publication of CN110959038A publication Critical patent/CN110959038A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/08—Reducing the nucleic acid content
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C12N9/1062—Sucrose synthase (2.4.1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01103—Alizarin 2-beta-glucosyltransferase (2.4.1.103)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y117/00—Oxidoreductases acting on CH or CH2 groups (1.17)
    • C12Y117/04—Oxidoreductases acting on CH or CH2 groups (1.17) with a disulfide as acceptor (1.17.3)
    • C12Y117/04001—Ribonucleoside-diphosphate reductase (1.17.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01012—Cellulose synthase (UDP-forming) (2.4.1.12)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01015—Alpha,alpha-trehalose-phosphate synthase (UDP-forming) (2.4.1.15)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/03—Phosphoric monoester hydrolases (3.1.3)
    • C12Y301/03009—Glucose-6-phosphatase (3.1.3.9)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y504/00—Intramolecular transferases (5.4)
    • C12Y504/02—Phosphotransferases (phosphomutases) (5.4.2)
    • C12Y504/02002—Phosphoglucomutase (5.4.2.2)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Water Supply & Treatment (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201880032258.6A 2017-05-15 2018-05-15 酶产物 Pending CN110959038A (zh)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US201762506357P 2017-05-15 2017-05-15
US62/506,357 2017-05-15
US201762581880P 2017-11-06 2017-11-06
US62/581,880 2017-11-06
EP17200572 2017-11-08
EP17200572.0 2017-11-08
EP18162420 2018-03-16
EP18162420.6 2018-03-16
PCT/EP2018/062476 WO2018210794A1 (en) 2017-05-15 2018-05-15 Enzyme products

Publications (1)

Publication Number Publication Date
CN110959038A true CN110959038A (zh) 2020-04-03

Family

ID=62386387

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201880032258.6A Pending CN110959038A (zh) 2017-05-15 2018-05-15 酶产物

Country Status (8)

Country Link
US (1) US11286474B2 (https=)
EP (1) EP3625334A1 (https=)
JP (1) JP7262449B2 (https=)
CN (1) CN110959038A (https=)
AU (1) AU2018268305B2 (https=)
CA (1) CA3063289A1 (https=)
MX (1) MX2019013659A (https=)
WO (1) WO2018210794A1 (https=)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114854719A (zh) * 2021-02-04 2022-08-05 普莱柯生物工程股份有限公司 一种制备高效核酸酶的方法及其应用
CN115369104A (zh) * 2022-09-27 2022-11-22 四川新川义生物科技有限责任公司 一种精制胃蛋白酶的方法

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018210794A1 (en) 2017-05-15 2018-11-22 C-Lecta Gmbh Enzyme products
CN113302298B (zh) 2018-11-09 2025-02-25 银杏生物制品公司 罗汉果甙的生物合成
CN111849962A (zh) * 2019-04-25 2020-10-30 北京挑战农业科技有限公司 一种生物制剂中宿主残留rDNA的去除方法
EP3926039A1 (en) * 2020-06-17 2021-12-22 AB Enzymes GmbH Use of a nuclease for reducing the viscosity and/or preventing an increase in viscosity of a fermentation broth
CN113604414A (zh) * 2021-08-23 2021-11-05 新乡医学院 生产天麻素的重组基因工程菌、构建方法及应用

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK210085D0 (da) * 1985-05-10 1985-05-10 Benzon As Alfred Fremgangsmaade til fremstilling af bakterielle enzymer
EP0252588A2 (en) * 1986-05-12 1988-01-13 Smithkline Beecham Corporation Process for the isolation and purification of P. falciparum CS protein expressed in recombinant E. coli, and its use as a vaccine
JPH04354585A (ja) * 1991-05-31 1992-12-08 Kao Corp 発酵液の処理方法
JP2014171421A (ja) * 2013-03-07 2014-09-22 National Agriculture & Food Research Organization Ｄｎａ親和性物質含有組成物、ｄｎａ親和性物質の精製方法、ｄｎａ親和性物質含有組成物の製造方法およびｄｎａ親和性物質を精製するためのキット
CN104099310A (zh) * 2013-04-12 2014-10-15 杭州俊丰生物工程有限公司 一种重组核酸酶及其制备方法
CN106518956A (zh) * 2016-10-24 2017-03-22 苏州海狸生物医学工程有限公司 一种去除蛋白质纯化体系中核酸污染的方法
CN106520814A (zh) * 2016-12-28 2017-03-22 苏州旷世骏弛生物科技有限公司 一种热稳定性核酸酶及其制备方法

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5173418A (en) * 1985-05-10 1992-12-22 Benzon Pharma, A/S Production in Escherichia coli of extracellular Serratia spp. hydrolases
GB9815666D0 (en) 1998-07-17 1998-09-16 Smithkline Beecham Plc Novel compounds
JP4847036B2 (ja) 2005-03-30 2011-12-28 キヤノン株式会社 バスアクセスを調停する制御装置およびデータ処理装置の制御方法
CA2602944C (en) * 2005-04-11 2015-08-11 Crucell Holland B.V. Virus purification using ultrafiltration
DE102007027825A1 (de) 2007-06-13 2008-12-18 C-Lecta Gmbh Amidohydrolasen zur Aufbereitung von Nahrungs- oder Genussmitteln
US8916364B2 (en) 2010-10-12 2014-12-23 C-Lecta Gmbh Nitrilases
CN103012591B (zh) 2012-12-12 2014-05-14 武汉吉爱生物技术有限公司 抗Benzonase单克隆抗体、其制备方法和应用
CN121203988A (zh) 2014-04-22 2025-12-26 C-乐克塔股份有限公司 酮还原酶
CN105985968B (zh) * 2015-02-04 2019-07-23 金普诺安生物科技（苏州）有限公司 改进的广谱核酸内切酶及其工业生产方法
WO2016198665A1 (en) 2015-06-12 2016-12-15 C-Lecta Gmbh Transaminases
WO2017070624A1 (en) * 2015-10-22 2017-04-27 Modernatx, Inc. Tropical disease vaccines
WO2018210794A1 (en) 2017-05-15 2018-11-22 C-Lecta Gmbh Enzyme products

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK210085D0 (da) * 1985-05-10 1985-05-10 Benzon As Alfred Fremgangsmaade til fremstilling af bakterielle enzymer
EP0252588A2 (en) * 1986-05-12 1988-01-13 Smithkline Beecham Corporation Process for the isolation and purification of P. falciparum CS protein expressed in recombinant E. coli, and its use as a vaccine
JPH04354585A (ja) * 1991-05-31 1992-12-08 Kao Corp 発酵液の処理方法
JP2014171421A (ja) * 2013-03-07 2014-09-22 National Agriculture & Food Research Organization Ｄｎａ親和性物質含有組成物、ｄｎａ親和性物質の精製方法、ｄｎａ親和性物質含有組成物の製造方法およびｄｎａ親和性物質を精製するためのキット
CN104099310A (zh) * 2013-04-12 2014-10-15 杭州俊丰生物工程有限公司 一种重组核酸酶及其制备方法
CN106518956A (zh) * 2016-10-24 2017-03-22 苏州海狸生物医学工程有限公司 一种去除蛋白质纯化体系中核酸污染的方法
CN106520814A (zh) * 2016-12-28 2017-03-22 苏州旷世骏弛生物科技有限公司 一种热稳定性核酸酶及其制备方法

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
HIROKAZU TAKAHASHI ET AL.: "Preparation of Phi29 DNA Polymerase Free of Amplifiable DNA Using Ethidium Monoazide, an Ultraviolet-Free Light-Emitting Diode Lamp and Trehalose" *
LARA J. AUSUBEL ET AL.: "Production of CGMP-Grade Lentiviral Vectors" *
PAUL C. BLAINEY ET AL.: "Digital MDA for enumeration of total nucleic acid contamination" *
RICHARD R. BURGESS ET AL.: "Use of Polyethyleneimine in Purification of DNA-Binding Proteins" *
曹志飞等: "重组肠激酶在大肠杆菌中的发酵与纯化" *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114854719A (zh) * 2021-02-04 2022-08-05 普莱柯生物工程股份有限公司 一种制备高效核酸酶的方法及其应用
CN115369104A (zh) * 2022-09-27 2022-11-22 四川新川义生物科技有限责任公司 一种精制胃蛋白酶的方法
CN115369104B (zh) * 2022-09-27 2023-09-08 四川新川义生物科技有限责任公司 一种精制胃蛋白酶的方法

Also Published As

Publication number Publication date
US20200140838A1 (en) 2020-05-07
EP3625334A1 (en) 2020-03-25
AU2018268305B2 (en) 2022-10-06
AU2018268305A1 (en) 2019-10-10
MX2019013659A (es) 2020-01-13
JP7262449B2 (ja) 2023-04-21
WO2018210794A1 (en) 2018-11-22
US11286474B2 (en) 2022-03-29
CA3063289A1 (en) 2018-11-22
JP2020519312A (ja) 2020-07-02

Similar Documents

Publication Publication Date Title
CN110959038A (zh) 酶产物
Lekha et al. Comparative titres, location and properties of tannin acyl hydrolase produced by Aspergillus niger PKL 104 in solid-state, liquid surface an submerged fermentations
Kademi et al. Purification and characterization of a thermostable esterase from the moderate thermophile Bacillus circulans
Lu et al. Production of novel alkalitolerant and thermostable inulinase from marine actinomycete Nocardiopsis sp. DN-K15 and inulin hydrolysis by the enzyme
Naidoo et al. Purification and Characterization of an Endoinulinase from Xanthomonas campestris pv. phaseoli KM 24 Mutant
CN103108950B (zh) 新的细胞外分泌型核酸酶
Fleuri et al. Production, purification and application of extracellular chitinase from Cellulosimicrobium cellulans 191
Satheesh Kumar et al. Purification and characterization of highly thermostable α-amylase from thermophilic Alicyclobacillus acidocaldarius
Alves-Prado et al. Purification and characterization of a cyclomaltodextrin glucanotransferase from Paenibacillus campinasensis strain H69-3
CN101948854B (zh) 乳糖酶突变基因及其分泌表达方法和应用
Rajeshwara Identification, characterization of novel halophilic Bacillus Cereus Ms6: A Source for extra cellular a-amylase
WO2017217453A1 (ja) セルラーゼ
CN101429518A (zh) 耐高温木糖苷酶XynB2和编码该酶的基因与应用
CN105950591B (zh) 一种中性低温木聚糖酶CaXyn10A及其基因和应用
Boopathy et al. Production of α-galactosidase from Aspergillus foetidus MTCC 6322 by solid state fermentation and its application in soymilk hydrolysis
CN113993878A (zh) 用二价阳离子从发酵液中回收蛋白的方法
Abou-Dobara et al. Optimization and physiochemical properties of xylanase from Bacillus coagulans and Bacillus licheniformis
CN103966194A (zh) 一种分泌性能提高的碱性果胶酶突变体
EP1199367B1 (en) Polypeptides
Kiribayeva et al. Cloning, purification and study of the biochemical properties of α-amylase from Bacillus licheniformis T5 strain
JP7194112B2 (ja) 細胞結合型異種タンパク質を発現する組換え酵母宿主細胞
Jha et al. Optimization of Solid State Production of Antileukemic Enzyme (L-Asparaginase) by E. coli K-12 using Taguchi Doe and Three Phase based Recovery
Kameshnee Naidoo et al. Purification and characterization of an endoinulinase from Xanthomonas campestris pv. phaseoli KM 24 mutant.
Joshi et al. Optimization of cultural conditions and partial characterization of alkaline amylase from Bacillus
JP2017035001A (ja) 耐熱性キシラナーゼ

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination