CN110839651B - Vitis heyneana rooting agent and Vitis heyneana cutting seedling method - Google Patents

Vitis heyneana rooting agent and Vitis heyneana cutting seedling method Download PDF

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CN110839651B
CN110839651B CN201911148328.7A CN201911148328A CN110839651B CN 110839651 B CN110839651 B CN 110839651B CN 201911148328 A CN201911148328 A CN 201911148328A CN 110839651 B CN110839651 B CN 110839651B
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water
pipe
passing
branches
cutting
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CN110839651A (en
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韩佳宇
张瑛
郭荣荣
曹雄军
林玲
白先进
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Guangxi Zhuang Nationality Autonomous Region Academy of Agricultural Sciences
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N61/00Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
    • A01N61/02Mineral oils; Tar oils; Tar; Distillates, extracts or conversion products thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00Cultivation of hops, vines, fruit trees, or like trees
    • A01G17/02Cultivation of hops or vines
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G2/00Vegetative propagation
    • A01G2/10Vegetative propagation by means of cuttings
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/10Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
    • A01G24/12Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • A01G24/22Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
    • A01G24/25Dry fruit hulls or husks, e.g. chaff or coir
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/10Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/36Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/48Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
    • A01N43/501,3-Diazoles; Hydrogenated 1,3-diazoles
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds

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Abstract

The invention belongs to the technical field of grape planting, and particularly discloses a downy grape rooting agent and a downy grape cutting seedling method. The grape rooting agent is mainly prepared from naphthylacetic acid, 5-methyl-3-p-chlorophenyl-2-thiohydantoin, proline, bamboo vinegar and ammonium bicarbonate, and can effectively promote the root growth of the cuttage branches of the downy grapes. The invention also provides a cutting seedling method of the downy grape, which is assisted by the water and air passing device, wherein the water and air passing device comprises a regulating device, an air pump, a water pump, a humidity sensor and a water and air passing composite pipe, and the cutting seedling method effectively promotes the root growth of the cutting branches and the survival rate of the cutting branches by combining a rooting agent and a special water and air passing device.

Description

Vitis heyneana rooting agent and Vitis heyneana cutting seedling method
[ technical field ] A method for producing a semiconductor device
The invention relates to the technical field of grape planting, in particular to a downy grape rooting agent and a downy grape cutting seedling method.
[ background of the invention ]
The wild grape has strong adaptability, can green barren mountains and slopes, prevent stony desertification, restore the ecological environment, develop the economy of rocky mountains and hilly areas, and is a wild fruit tree with great development potential. Guangxi as the country of the Chinese wild downy grape and the origin of the Chinese wild downy grape wine, the planting area of the downy grape is increased year by year. A new amphiprotic downy grape variety 'wild brewing No. 2' newly bred in 2010 is popularized and planted in large areas in city counties such as Luo city county, Duan county, Dahua county, Liuzhou city, Guilin city and the like in Guangxi river basin, and the total area is over 3300hm2The method makes the wild grape break through the artificial planting area and yield, and has important significance for the development of wild grape industry in Guangxi province and even south China.
However, the downy grapes are mainly propagated by tissue culture seedlings at present, and the problem of difficult rooting still exists in hardwood cutting.
[ summary of the invention ]
The invention aims to: aiming at the problems, the downy grape rooting agent and the cutting seedling method of the downy grape are provided. The grape rooting agent can effectively promote the root growth of the cuttage branches of the downy grapes, and the cuttage seedling culture method further effectively improves the rooting amount and the survival rate of the cuttage branches of the downy grapes by combining the ventilation and water-passing device and the rooting agent.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows:
the downy grape rooting agent comprises the following raw material components: 200mg/L of naphthylacetic acid, 5-10mg/L of 5-methyl-3-p-chlorophenyl-2-thiohydantoin, 35-50mg/L of proline, 5-15mg/L of bamboo vinegar and 50-80mg/L of ammonium bicarbonate.
Further, the vitis heyneana rooting agent comprises the following raw material components: 180mg/L of naphthylacetic acid, 8mg/L of 5-methyl-3-p-chlorophenyl-2-thiohydantoin, 45mg/L of proline, 12mg/L of bamboo vinegar liquid and 65mg/L of ammonium bicarbonate.
The invention also provides a downy grape cuttage seedling method, which is used for treating the cuttage branches of the downy grapes by using the rooting agent for the downy grapes.
Further, the method for raising the seedlings of the downy grapes through cutting comprises the following steps:
(1) preparing a seedbed: the length and width of the seedbed are 10.0mX0.2m, a substrate with the thickness of 5-10cm is covered at the bottom of the seedbed, a water and air passing device is arranged on the first layer of substrate, and a substrate with the thickness of 5-10cm is covered on the water and air passing device, so that the seedbed is obtained;
(2) preparing cuttage branches: selecting an annual branch which is free of diseases and insect pests, free of damage, fully mature and full in tooth eyes, and shearing a double-bud stem section with the length of 8-10cm to obtain a cutting branch; binding every 6 cutting branches into a bundle, aligning the lower ends of the cutting branches, immersing the bundled cutting branches in water for 20-24h, taking out the cutting branches, draining, and then putting the cutting branches into the downy grape rooting agent for soaking for 20-30min to obtain treated cutting branches for later use;
(3) cuttage: inserting the treated cutting branches into the substrate of the seedbed and compacting the substrate in the morning of sunny days,
(4) cuttage management: and after cuttage, controlling the water-passing and ventilating device to ventilate and feed water to the seedbed.
Further, in the step (1), the water and air passing device comprises a regulating device, an air pump, a water pump, a humidity sensor and a water and air passing composite pipe, the regulating device is respectively connected with the humidity sensor, the air pump and the water pump, and the water and air passing composite pipe is snakelike and embedded in the seedbed; the water and air passing composite pipe comprises an inner pipe and an outer pipe sleeved outside the inner pipe, the inner diameter of the outer pipe is 2-3 times of the outer diameter of the inner pipe, the head end of the outer pipe is in sealing connection with the outer wall, close to the head end, of the inner pipe, and the tail end of the outer pipe is in sealing connection with the outer wall, close to the tail end, of the inner pipe; a baffle plate is arranged in the inner pipe, two side edges of the baffle plate respectively penetrate through the pipe wall of the inner pipe and are hermetically connected with the inner walls of two opposite sides of the outer pipe, the inner pipe is divided into a water-passing inner pipe and a ventilation inner pipe by the clapboard, the tail ends of the water-passing inner pipe and the ventilation inner pipe are sealed, the head end of the water inner pipe is integrally connected with a water joint, the water joint is connected with the water pump, the head end of the ventilation inner pipe is integrally connected with the ventilation joint which is connected with the air pump, the pipe wall of the water-passing inner pipe is provided with a water-passing inner hole, the pipe wall of the ventilation inner pipe is provided with a ventilation inner hole, meanwhile, the outer pipe is divided into a water-passing outer pipe and a ventilating outer pipe by the partition plate, water-passing outer holes are formed in the pipe wall of the side edge of the water-passing outer pipe and the pipe wall of the bottom of the water-passing outer pipe, and ventilating outer holes are formed in the pipe wall of the side edge of the ventilating outer pipe and the pipe wall of the bottom of the ventilating outer pipe.
Furthermore, the regulation and control device comprises a controller, a timer and a control panel, wherein the timer, the control panel, the humidity sensor, the air pump and the water pump are all connected with the controller.
The raw materials of the invention are as follows:
the cuttage branches of the downy grapes are purchased in a certain vineyard in Luo city county of river pool, Guangxi province; naphthylacetic acid is available from Ling scientific Co., Ltd, Hainan vast; 5-methyl-3-p-chlorophenyl-2-thiohydantoin was obtained from Shandong Minye Chemicals, Inc.; proline was purchased from Hebei Huayang Biotech limited; bamboo vinegar was purchased from Zhengzhou Yinong Biotechnology Ltd; ammonium bicarbonate was purchased from bioengineering, ltd, boon, west ampere.
In summary, due to the adoption of the technical scheme, the invention has the beneficial effects that:
firstly, the components of the rooting agent of the invention are mutually matched to generate obvious synergistic effect, the cuttage survival rate of the cuttage branches of the downy grapes is effectively improved, and the root system growth of the downy grapes is effectively promoted, while the root system of the cuttage branches is not obviously promoted only by adopting a single component or only adopting a plurality of components in the rooting agent of the invention, for example, if the cuttage branches are treated by only adopting the rooting agent made of 5-methyl-3-p-chlorophenyl-2-thiohydantoin, proline and naphthylacetic acid, the average root thickness and the average root number of the cuttage branches and the cuttage branches treated by only adopting the naphthylacetic acid are respectively improved by 0.25-0.28 percent and 0.13-0.17 percent, the promotion effect is not obvious, and only the cuttage branches treated by only adopting 5-methyl-3-p-chlorophenyl-2-thiohydantoin, the root system of the invention are only matched with each other components, so that obvious synergistic effect can be generated, and the, The bamboo vinegar solution and the naphthylacetic acid are used for treating the cuttage branches, although the rooting rate of the cuttage branches is promoted to a certain extent, the average root length of the cuttage branches is even 0.18mm lower than that of the cuttage branches treated by only the naphthylacetic acid.
Secondly, the water and air passing device is arranged in the seedbed, so that the seedbed keeps proper humidity and oxygen content, the cuttage branches can effectively absorb moisture and oxygen, the root growth of the cuttage branches is effectively promoted, and the cuttage survival rate of the cuttage branches is improved. The water and air communicating composite pipe is arranged in a snake shape and can be uniformly laid in the seedbed, so that the uniformity of the humidity and the oxygen content in the seedbed is improved; the outer pipe is sleeved outside the inner pipe, and the through hole formed in the outer pipe is staggered with the through hole formed in the inner pipe, so that the phenomenon that the through hole in the inner pipe is blocked by the substrate on the seedbed entering the inner pipe can be effectively avoided; further, divide into breather pipe and water service pipe through the baffle with the pipeline, a pipeline of breather pipe and water service pipe sharing promptly, is convenient for lay the installation, but the water-logging with ventilate mutually independent, each other not influence, for chinese utility model patent CN 205623606U store drip irrigation system's liquid manure gas integration equipment in based on the underground cave water vapour sharing passageway, the practicality is stronger.
[ description of the drawings ]
FIG. 1 is a schematic view showing the structure of a water-through/air-vent apparatus according to the present invention.
FIG. 2 is a schematic view showing the structure of the water/air composite pipe shown in FIG. 1.
Fig. 3 is a schematic view of a connection structure of the control structure of the present invention.
The main reference symbols in the drawings are as follows:
1-regulating device, 2-air pump, 3-water pump, 4-humidity sensor, 5-water and air passing composite pipe, 51-air passing joint, 52-water passing joint, 53-inner pipe, 531-air passing inner pipe, 532-water passing inner pipe, 54-outer pipe, 541-air passing outer pipe, 542-water passing outer pipe, 55-water passing inner hole, 56-air passing inner hole, 57-water passing outer hole, 58-air passing outer hole and 59-clapboard.
[ detailed description ] embodiments
The invention will now be further described with reference to specific examples.
The water-passing and ventilating device used in the following embodiments has the following specific structure:
as shown in fig. 1 and 3, in a preferred embodiment of the present invention, the water and air ventilation device includes a control device 1, an air pump 2, a water pump 3, a humidity sensor 4 and a water and air ventilation composite pipe 5, the control device 1 includes a controller, a timer, a control panel and a power supply, the timer, the control panel, the power supply, the humidity sensor 4, the air pump 2 and the water pump 3 are all connected to the controller, the power supply is used for supplying power to the water and air ventilation device, the timer is used for timing control of the operating time of the air pump 2, the humidity sensors 4 are uniformly inserted into the seedbed and transmit the sensed humidity signal to the controller, and then the controller controls the operating state of the water pump 3 according to the received humidity signal; the water and air communicating composite pipe 5 is snakelike and is buried in the seedbed and used for supplying water and air to the seedbed, so that the propagation and development of the cutting branches of the downy grapes are promoted.
Further referring to fig. 2, the water and air passing composite pipe 5 includes an inner pipe 53 and an outer pipe 54 sleeved outside the inner pipe 53, wherein the inner diameter of the outer pipe 54 is 2-3 times the outer diameter of the inner pipe 53, that is, the outer wall of the inner pipe 53 and the inner wall of the outer pipe 54 are not in contact with each other but have a certain interval, the head end of the outer pipe 54 is connected with the outer wall of the inner pipe 53 near the head end thereof in a sealing manner, and the tail end of the outer pipe 54 is connected with the outer wall of the tail end of the inner pipe 53 in a sealing manner. A partition plate 59 is arranged in the inner pipe 53, and two side edges of the partition plate 59 respectively penetrate through the pipe wall of the inner pipe 53 and are connected with the inner walls of two opposite sides of the outer pipe 54 in a sealing manner, namely, one side edge of the partition plate 59 is connected with the inner wall of the outer pipe 51 at the bottom side in a sealing manner, and the other side edge penetrates through the inner pipe 53 and is connected with the inner wall of the outer pipe 54 at the top side in a sealing manner; the inner pipe 53 is divided into a left water-passing inner pipe 532 and a right air-passing inner pipe 531 by the partition plate 59, the ends of the water-passing inner pipe 532 and the air-passing inner pipe 531 are sealed, the head end of the water-passing inner pipe 532 is integrally connected with a water-passing joint 52, the water-passing joint 52 is connected with the water pump 3, the head end of the air-passing inner pipe 531 is integrally connected with an air-passing joint 51, the air-passing joint 51 is connected with the air pump 2, a plurality of water-passing inner holes 55 are formed in the pipe wall of the water-passing inner pipe 532, and a plurality of air-passing inner holes 56 are formed in the pipe wall of the air-; meanwhile, the partition plate 59 partitions the outer pipe 54 into a left-right water passing outer pipe 542 and a ventilating outer pipe 541, the side wall of the water passing outer pipe 542 and the bottom wall of the water passing outer pipe are provided with a plurality of water passing outer holes 57, the outer water passing holes 57 and the inner water passing holes 55 are arranged in a staggered manner, the aperture of the outer water passing holes 57 is smaller than that of the inner water passing holes 55, the side wall and the bottom wall of the ventilation outer tube 542 are provided with ventilation outer holes 58, the ventilation outer holes 58 and the ventilation inner holes 56 are arranged in a staggered mode, the aperture of each ventilation outer hole 58 is smaller than that of each ventilation inner hole 56, this arrangement effectively prevents the matrix particles covering the water and air passing composite pipe 5 from entering the air passing outer pipe 541 and the water passing outer pipe 542, and the ventilation inner hole 56 and the water through inner hole 55 are blocked, thereby being beneficial to the smooth operation of the water and air through composite pipe 5.
The working process of the water-passing and air-ventilating device is as follows:
pressing down a power supply, starting the water and air passing device, and setting an expected humidity value, an air passing time point and air passing duration of the seedbed through the control panel; the humidity sensor 4 sends the bed humidity detected in real time to the controller, when the detected real-time humidity value is smaller than an expected humidity value, the controller controls the water pump 3 to start to operate to convey tap water to the water through inner pipe 532, the tap water sequentially passes through the water through inner pipe 532, the water through inner hole 55, the water through outer pipe 542 and the water through outer hole 57 to enter the bed, so that the effect of moistening the bed is achieved, and when the detected real-time humidity value is the same as the expected humidity value, the controller controls the water pump 3 to stop operating, so that water supply to the bed is stopped; when the ventilation time point is reached, the controller controls the air pump 2 to start to deliver air/oxygen to the ventilation inner tube 531, the air/oxygen sequentially passes through the ventilation inner tube 531, the ventilation inner hole 56, the ventilation outer tube 541 and the ventilation outer hole 58 to enter the seedbed, and starts to supply air to the seedbed, and at the same time, the controller controls the timer to start timing, and when the time of the timer reaches a preset ventilation time length, the controller controls the air pump 2 to stop operating, so that the ventilation to the seedbed is stopped.
The controller, the timer, the humidity sensor 4, the air pump 2, the water pump 3, the control panel, the type, structure and action principle of the power supply, the electric connection method among the controller and the controller belong to the prior art, and are not described herein for saving space.
Example 1
The downy grape rooting agent comprises the following raw material components: 150mg/L of naphthylacetic acid, 5mg/L of 5-methyl-3-p-chlorophenyl-2-thiohydantoin, 35mg/L of proline, 5mg/L of bamboo vinegar liquid and 50mg/L of ammonium bicarbonate; and (3) mixing and stirring the raw material components uniformly to obtain the downy grape rooting agent.
A downy grape cuttage seedling raising method applying the downy grape rooting agent comprises the following steps:
(1) preparing a seedbed: the length and width of the seedbed are 10.0mX0.2m, a substrate with the thickness of 5cm is covered at the bottom of the seedbed, a water and air communicating composite pipe of an air breather is laid on the first layer of substrate, and a substrate with the thickness of 5cm is covered on the water and air breather to obtain the seedbed; the matrix is mainly prepared by mixing river sand, coconut coir and plant ash according to the weight ratio of 6:1: 1;
(2) preparing cuttage branches: selecting an annual branch which is free of diseases and insect pests, free of damage, fully mature and full in tooth eyes, and shearing a double-bud stem section with the length of 8cm to obtain a cutting branch; binding every 6 cutting branches into a bundle, aligning the lower ends of the cutting branches, immersing the bundled cutting branches in water for 20 hours, taking out the cutting branches, draining, and then putting the cutting branches into the downy grape rooting agent for soaking for 20 minutes to obtain treated cutting branches for later use;
(3) cuttage: inserting the treated cutting branches into the substrate of the seedbed and compacting the substrate in the morning of sunny days,
(4) cuttage management: and after cuttage, controlling the water-passing and ventilating device to ventilate and feed water to the seedbed.
Example 2
The downy grape rooting agent comprises the following raw material components: 180mg/L of naphthylacetic acid, 8mg/L of 5-methyl-3-p-chlorophenyl-2-thiohydantoin, 45mg/L of proline, 12mg/L of bamboo vinegar liquid and 65mg/L of ammonium bicarbonate; and (3) mixing and stirring the raw material components uniformly to obtain the downy grape rooting agent.
A downy grape cuttage seedling raising method applying the downy grape rooting agent comprises the following steps:
(1) preparing a seedbed: the length and width of the seedbed are 10.0mX0.2m, a substrate with the thickness of 8cm is covered at the bottom of the seedbed, a water and air communicating composite pipe of an air breather is laid on the first layer of substrate, and a substrate with the thickness of 8cm is covered on the water and air breather to obtain the seedbed; the matrix is mainly prepared by mixing river sand, coconut coir and plant ash according to the weight ratio of 6:1.5: 1.2;
(2) preparing cuttage branches: selecting an annual branch which is free of diseases and insect pests, free of damage, fully mature and full in tooth eyes, and shearing a double-bud stem section with the length of 9cm to obtain a cutting branch; binding every 6 cutting branches into a bundle, aligning the lower ends of the cutting branches, immersing the bundled cutting branches in water for 22 hours, taking out the cutting branches, draining, and then putting the cutting branches into the downy grape rooting agent for soaking for 25 minutes to obtain treated cutting branches for later use;
(3) cuttage: inserting the treated cutting branches into the substrate of the seedbed and compacting the substrate in the morning of sunny days,
(4) cuttage management: and after cuttage, controlling the water-passing and ventilating device to ventilate and feed water to the seedbed.
Example 3
The downy grape rooting agent comprises the following raw material components: 200mg/L of naphthylacetic acid, 10mg/L of 5-methyl-3-p-chlorophenyl-2-thiohydantoin, 50mg/L of proline, 15mg/L of bamboo vinegar liquid and 80mg/L of ammonium bicarbonate; and (3) mixing and stirring the raw material components uniformly to obtain the downy grape rooting agent.
A downy grape cuttage seedling raising method applying the downy grape rooting agent comprises the following steps:
(1) preparing a seedbed: the length and width of the seedbed are 10.0mX0.2m, the bottom of the seedbed is covered with a substrate with the thickness of 10cm, a water and air communicating composite pipe of an air breather is laid on the first layer of substrate, and the water and air breather is covered with a substrate with the thickness of 10cm to obtain the seedbed; the matrix is mainly prepared by mixing river sand, coconut coir and plant ash according to the weight ratio of 6:2: 1.5;
(2) preparing cuttage branches: selecting an annual branch which is free of diseases and insect pests, free of damage, fully mature and full in tooth eyes, and shearing a double-bud stem section with the length of 10cm to obtain a cutting branch; binding every 6 cutting branches into a bundle, aligning the lower ends of the cutting branches, immersing the bundled cutting branches in water for 24 hours, taking out the cutting branches, draining, and then putting the cutting branches into the downy grape rooting agent for soaking for 30 minutes to obtain treated cutting branches for later use;
(3) cuttage: inserting the treated cutting branches into the substrate of the seedbed and compacting the substrate in the morning of sunny days,
(4) cuttage management: and after cuttage, controlling the water-passing and ventilating device to ventilate and feed water to the seedbed.
Effect verification
1. Effect of rooting fluids on cuttings
Experimental groups 1 to 3: adopting the cuttage branch obtained after cuttage in the embodiment 1-3 of the invention;
control group 1: the method for cutting the cuttage branches of the group is basically the same as that of the embodiment 1, and the difference is only that: the hairy grape rooting agent is only prepared from naphthylacetic acid;
control group 2: the method for cutting the cuttage branches of the group is basically the same as that of the embodiment 1, and the difference is only that: the downy grape rooting agent is only prepared from 5-methyl-3-p-chlorophenyl-2-thiohydantoin;
control group 3: the method for cutting the cuttage branches of the group is basically the same as that of the embodiment 1, and the difference is only that: the hairy grape rooting agent is only prepared from proline;
control group 4: the method for cutting the cuttage branches of the group is basically the same as that of the embodiment 1, and the difference is only that: the downy grape rooting agent is only prepared from bamboo vinegar;
control group 5: the method for cutting the cuttage branches of the group is basically the same as that of the embodiment 1, and the difference is only that: the hairy grape rooting agent is only prepared from ammonium bicarbonate.
Blank group: the method for cutting the cuttage branches of the group is basically the same as that of the embodiment 1, and the difference is only that: the hairy grape rooting agent is replaced by clear water.
The experiments are all carried out in a certain field of grape institute of agricultural science and academy of Guangxi province, and the number of the cuttage branches in each group is 120. And (3) carrying out cuttage on the processed cuttage branches of each group in 18 th of 2019 in 4 th of month, surveying and recording the growth condition of the cuttage branches of the vitis amurensis of each group in 19 th of 2019 in 3 months after cuttage (the root number, the root length and the root thickness are average numbers).
Wherein:
the survival rate is the number of surviving cuttage branches/total number of cuttage branches is 100%, and whether the cuttage branches survive or not is judged by professional technicians according to the judgment standard of the prior art;
the rooting rate is 100% of the number of the rooted cuttage branches/the total number of the cuttage branches, in the experiment, if the dead cuttage branches have roots, the dead cuttage branches are included in the number of the rooted cuttage branches, and whether the cuttage branches root or not is judged by technical personnel;
the average rooting number is the total rooting number of the rooting cuttage branches/the number of the rooting cuttage branches, and the rooting number is obtained by counting the number of technicians;
the root length and the heel thickness are obtained by measuring with calipers with corresponding precision and then calculating by taking the average value.
The statistical results are shown in table 1:
TABLE 1 growth of the cuttings of each group
Group of Survival rate/% Rooting percentage/%) Root number/plant Root length/mm Root thickness/mm
Experimental group 1 95.0 97.5 4.78 13.78 0.49
Experimental group 2 95.8 98.3 4.49 14.11 0.51
Experimental group 3 95.0 96.7 4.37 13.62 0.47
Control group 1 79.2 82.5 1.87 8.87 0.38
Control group 2 49.2 54.2 1.20 5.24 0.25
Control group 3 55.8 60.0 1.31 5.34 0.24
Control group 4 55.0 58.3 1.53 4.98 0.21
Control group 5 48.3 50.8 1.11 5.37 0.19
Blank group 47.5 53.3 1.05 5.05 0.23
From table 1, the survival rate, rooting number, root length and root thickness of the cuttage branches of the vitis amurensis of the experimental groups 1 to 3 are obviously superior to those of the control groups 1 to 5 and the blank groups, and various indexes of the cuttage branches of the control groups 2 to 5 are not much different from those of the blank groups, even some indexes are different from those of the blank groups, so that the 5-methyl-3-p-chlorophenyl-2-thiohydantoin, the proline, the bamboo vinegar, the ammonium bicarbonate and the naphthylacetic acid can generate a synergistic effect, thereby effectively improving the survival rate of the cuttage branches of the vitis amurensis and effectively promoting the root growth of the cuttage branches of the vitis amurensis.
The above description is intended to describe in detail the preferred embodiments of the present invention, but the embodiments are not intended to limit the scope of the claims of the present invention, and all equivalent changes and modifications made within the technical spirit of the present invention should fall within the scope of the claims of the present invention.

Claims (5)

1. The downy grape rooting agent is characterized by comprising the following raw material components: 200mg/L of naphthylacetic acid, 5-10mg/L of 5-methyl-3-p-chlorophenyl-2-thiohydantoin, 35-50mg/L of proline, 5-15mg/L of bamboo vinegar and 50-80mg/L of ammonium bicarbonate.
2. The Vitis heyneana rooting agent according to claim 1, which comprises the following raw material components: 180mg/L of naphthylacetic acid, 8mg/L of 5-methyl-3-p-chlorophenyl-2-thiohydantoin, 45mg/L of proline, 12mg/L of bamboo vinegar liquid and 65mg/L of ammonium bicarbonate.
3. A downy grape cuttage seedling raising method is characterized in that: treating the Vitis heyneana cutting branches by using the Vitis heyneana rooting agent according to claim 1 or 2.
4. The Vitis heyneana cutting seedling raising method according to claim 3, characterized by comprising the following steps:
(1) preparing a seedbed: the length and width of the seedbed are 10.0m multiplied by 0.2m, a substrate with the thickness of 5-10cm is covered at the bottom of the seedbed, a water and air passing device is arranged on the substrate, and the substrate with the thickness of 5-10cm is covered on the water and air passing device to obtain the seedbed;
(2) preparing cuttage branches: selecting an annual branch which is free of diseases and insect pests, free of damage, fully mature and full in tooth eyes, and shearing a double-bud stem section with the length of 8-10cm to obtain a cutting branch; binding every 6 cutting branches into a bundle, aligning the lower ends of the cutting branches, immersing the bundled cutting branches in water for 20-24h, taking out the cutting branches, draining, and then putting the cutting branches into the downy grape rooting agent for soaking for 20-30min to obtain treated cutting branches for later use;
(3) cuttage: inserting the treated cutting branches into the substrate of the seedbed and compacting the substrate in the morning of sunny days,
(4) cuttage management: after cuttage, controlling the water-passing and ventilating device to ventilate and feed water to the seedbed;
the water and air passing device comprises a regulating device, an air pump, a water pump, a humidity sensor and a water and air passing composite pipe, the regulating device comprises a controller, a timer and a control panel, the timer, the control panel, the humidity sensor, the air pump and the water pump are all connected with the controller, and the water and air passing composite pipe is snakelike and is embedded in the seedbed; the water and air passing composite pipe comprises an inner pipe and an outer pipe sleeved outside the inner pipe, the inner diameter of the outer pipe is 3 times of the outer diameter of the inner pipe, the head end of the outer pipe is in sealing connection with the outer wall of the inner pipe close to the head end of the outer pipe, and the tail end of the outer pipe is in sealing connection with the outer wall of the tail end of the inner pipe; a baffle plate is arranged in the inner pipe, two side edges of the baffle plate respectively penetrate through the pipe wall of the inner pipe and are hermetically connected with the inner walls of two opposite sides of the outer pipe, the inner pipe is divided into a water-passing inner pipe and a ventilation inner pipe by the clapboard, the tail ends of the water-passing inner pipe and the ventilation inner pipe are sealed, the head end of the water inner pipe is integrally connected with a water joint, the water joint is connected with the water pump, the head end of the ventilation inner pipe is integrally connected with a ventilation joint which is connected with the air pump, the pipe wall of the water-passing inner pipe is provided with a water-passing inner hole, the pipe wall of the ventilation inner pipe is provided with a ventilation inner hole, meanwhile, the outer pipe is divided into a water-passing outer pipe and a ventilating outer pipe by the partition plate, water-passing outer holes are formed in the pipe wall of the side edge of the water-passing outer pipe and the pipe wall of the bottom of the water-passing outer pipe, and ventilating outer holes are formed in the pipe wall of the side edge of the ventilating outer pipe and the pipe wall of the bottom of the ventilating outer pipe; the outer water through hole and the inner water through hole are arranged in a staggered mode, the aperture of the outer water through hole is smaller than that of the inner water through hole, the outer ventilation hole and the inner ventilation hole are arranged in a staggered mode, and the aperture of the outer ventilation hole is smaller than that of the inner ventilation hole.
5. The cutting seedling method of Vitis heyneana according to claim 4, wherein in step (1), the substrate is mainly prepared by mixing river sand, coconut coir and plant ash according to the weight ratio of 6:1-2: 1-1.5.
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