CN110770351A - β-葡萄糖苷酶的用途及用其制备宝藿苷Ⅰ的方法 - Google Patents

β-葡萄糖苷酶的用途及用其制备宝藿苷Ⅰ的方法 Download PDF

Info

Publication number
CN110770351A
CN110770351A CN201880038008.3A CN201880038008A CN110770351A CN 110770351 A CN110770351 A CN 110770351A CN 201880038008 A CN201880038008 A CN 201880038008A CN 110770351 A CN110770351 A CN 110770351A
Authority
CN
China
Prior art keywords
baohuoside
glucosidase
solution
epimedin
preparation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201880038008.3A
Other languages
English (en)
Inventor
傅荣昭
刘立辉
曹磊
刘滔滔
郭杏林
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BONTAC BIO-ENGINEERING (SHENZHEN) Co.,Ltd.
Original Assignee
Jiangxi Bang Tai Green Biosynthesis Eco Industrial Park Development Co Ltd
Bangtai Bioengineering (shenzhen) Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Jiangxi Bang Tai Green Biosynthesis Eco Industrial Park Development Co Ltd, Bangtai Bioengineering (shenzhen) Co Ltd filed Critical Jiangxi Bang Tai Green Biosynthesis Eco Industrial Park Development Co Ltd
Publication of CN110770351A publication Critical patent/CN110770351A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/60—Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

提供了一种使用β‑葡萄糖苷酶制备宝藿苷Ⅰ的方法，包括用β‑葡萄糖苷酶催化底物水解制备宝藿苷Ⅰ，底物为淫羊藿提取物、淫羊藿苷、朝藿定A、朝藿定B、箭藿苷A和箭藿苷B中的任意一种或多种，所述β‑葡萄糖苷酶具有如SEQ ID NO：2所示的氨基酸序列。该方法能够将淫羊藿、朝藿定A、朝藿定B、箭藿苷A及箭藿苷B全部水解成宝藿苷Ⅰ，提高了淫羊藿提取物的有效利用率，大大降低了宝藿苷Ⅰ的制备成本。还提供了所述β‑葡萄糖苷酶以及含有所述β‑葡萄糖苷酶基因的微生物菌株在催化淫羊藿提取物、淫羊藿苷、朝藿定A、朝藿定B、箭藿苷A和箭藿苷B中的任意一种或多种水解，制备宝藿苷Ⅰ中的应用。

Description

PCT国内申请，说明书已公开。

Claims (12)

  1. PCT国内申请，权利要求书已公开。
CN201880038008.3A 2018-04-25 2018-04-25 β-葡萄糖苷酶的用途及用其制备宝藿苷Ⅰ的方法 Pending CN110770351A (zh)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2018/084475 WO2019205026A1 (zh) 2018-04-25 2018-04-25 β-葡萄糖苷酶的用途及用其制备宝藿苷Ⅰ的方法

Publications (1)

Publication Number Publication Date
CN110770351A true CN110770351A (zh) 2020-02-07

Family

ID=68294354

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201880038008.3A Pending CN110770351A (zh) 2018-04-25 2018-04-25 β-葡萄糖苷酶的用途及用其制备宝藿苷Ⅰ的方法

Country Status (2)

Country Link
CN (1) CN110770351A (zh)
WO (1) WO2019205026A1 (zh)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112063672A (zh) * 2020-09-23 2020-12-11 浙大宁波理工学院 一种利用外切葡聚糖酶制备淫羊藿次苷ⅱ的工艺
CN115399405A (zh) * 2022-11-01 2022-11-29 四川合泰新光生物科技有限公司 一种宝藿苷i葡聚糖包合物的用途
CN117089465A (zh) * 2023-08-22 2023-11-21 陕西省微生物研究所 一种疣梗曲霉及应用

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102311984A (zh) * 2011-07-05 2012-01-11 贾晓斌 一种从淫羊藿制备宝藿苷ⅰ的方法
CN102311985A (zh) * 2011-07-05 2012-01-11 贾晓斌 一种宝藿苷ⅰ的制备方法
CN102899300A (zh) * 2011-07-28 2013-01-30 中国科学院上海生命科学研究院 一种新型耐高温β-葡萄糖苷酶及其编码基因和应用
CN106148454A (zh) * 2015-03-24 2016-11-23 北京珅奥基医药科技有限公司 一种宝藿苷ⅰ的制备方法
CN107164436A (zh) * 2017-05-12 2017-09-15 南京林业大学 β‑葡萄糖苷酶在转化淫羊藿总黄酮制备宝霍苷I中的应用

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102311984A (zh) * 2011-07-05 2012-01-11 贾晓斌 一种从淫羊藿制备宝藿苷ⅰ的方法
CN102311985A (zh) * 2011-07-05 2012-01-11 贾晓斌 一种宝藿苷ⅰ的制备方法
CN102899300A (zh) * 2011-07-28 2013-01-30 中国科学院上海生命科学研究院 一种新型耐高温β-葡萄糖苷酶及其编码基因和应用
CN106148454A (zh) * 2015-03-24 2016-11-23 北京珅奥基医药科技有限公司 一种宝藿苷ⅰ的制备方法
CN107164436A (zh) * 2017-05-12 2017-09-15 南京林业大学 β‑葡萄糖苷酶在转化淫羊藿总黄酮制备宝霍苷I中的应用

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
WANG,Q.等: "beta-glucosidase [uncultured bacterium contig00059]", 《GENBANK ACCESSION NO: AGS52251.1》 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112063672A (zh) * 2020-09-23 2020-12-11 浙大宁波理工学院 一种利用外切葡聚糖酶制备淫羊藿次苷ⅱ的工艺
CN115399405A (zh) * 2022-11-01 2022-11-29 四川合泰新光生物科技有限公司 一种宝藿苷i葡聚糖包合物的用途
CN115399405B (zh) * 2022-11-01 2023-03-14 四川合泰新光生物科技有限公司 一种宝藿苷i葡聚糖包合物的用途
CN117089465A (zh) * 2023-08-22 2023-11-21 陕西省微生物研究所 一种疣梗曲霉及应用
CN117089465B (zh) * 2023-08-22 2024-05-03 陕西省微生物研究所 一种疣梗曲霉及应用

Also Published As

Publication number Publication date
WO2019205026A1 (zh) 2019-10-31

Similar Documents

Publication Publication Date Title
CN110770351A (zh) β-葡萄糖苷酶的用途及用其制备宝藿苷Ⅰ的方法
CN110770350B (zh) 一种利用β-葡萄糖苷酶制备宝藿苷I的方法
CN112210548A (zh) 一种表达α-L-鼠李糖苷酶的毕赤酵母及其制备方法和应用
CN103589702B (zh) 一种耐热性β-葡萄糖苷酶及其突变体的应用
CN105238826B (zh) β-葡萄糖苷酶的用途及用其制备黄芩素的方法
CN106148256B (zh) 产α-熊果苷的基因工程菌及其构建方法和应用
CN104784254A (zh) 一种生物酶法生产黄芩苷的提取方法
CN116042751A (zh) 利用木质纤维素衍生物生物合成高附加值化合物的方法
CN102351926B (zh) 一种牛蒡子苷的制备方法
KR20080033705A (ko) 캄페롤-3-ｏ-루티노사이드의 제조방법
CN101760483A (zh) 一种从新鲜虎杖中制备高纯度白藜芦醇的方法
CN102174620A (zh) 一种酶催化直接糖基化合成红景天苷的方法
CN101760487B (zh) 一种淫羊藿苷元的制备方法
CN103145670A (zh) 一种半合成制备木犀草素的新工艺
CN103180334A (zh) 制备芍药内酯苷和芍药苷的方法
CN106432387B (zh) 苦荞籽中芦丁和多糖的联合提取方法
CN104818262B (zh) 一种利用耐热β-葡萄糖苷酶及其突变体进行虎杖苷生物转化的方法
CN111848709B (zh) 一种新型异戊烯基黄酮类化合物及其制备方法和应用
CN106148449B (zh) 一种淫羊藿次苷i的制备方法
CN105949098A (zh) 一种从西兰花中提取萝卜硫素的制备方法
CN102643285A (zh) 一种从辛夷中制备木兰脂素的方法
CN112553264A (zh) 酶转化高效制备淫羊藿苷元的方法
CN107058408B (zh) 一种利用细菌转化和提取白藜芦醇的方法
CN102477055A (zh) 一种从大蓟中提取纯化柳穿鱼叶苷的方法
CN111875716A (zh) 一种高抗氧化活性山药多糖的提取及提纯方法

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
TA01 Transfer of patent application right
TA01 Transfer of patent application right

Effective date of registration: 20200716

Address after: 518102 Guangdong Shenzhen Baoan District Xixiang street the Peach Garden Gang the Peach Garden science and Technology Innovation Park third points Park 1 buildings 1 floors A-2

Applicant after: BONTAC BIO-ENGINEERING (SHENZHEN) Co.,Ltd.

Address before: 518102 Guangdong Shenzhen Baoan District Xixiang street the Peach Garden Gang the Peach Garden science and Technology Innovation Park third points Park 1 buildings 1 floors A-2

Applicant before: BONTAC BIO-ENGINEERING (SHENZHEN) Co.,Ltd.

Applicant before: JIANGXI BONTAC GREEN BIOCATALYSIS ECOINDUSTRIAL PARK Co.,Ltd.

RJ01 Rejection of invention patent application after publication
RJ01 Rejection of invention patent application after publication

Application publication date: 20200207