CN110585102A

CN110585102A - Blackhead-derived plant composition, blackhead-derived liquid and preparation method

Info

Publication number: CN110585102A
Application number: CN201911047144.1A
Authority: CN
Inventors: 龚德明; 范玉菡; 温伟红
Original assignee: Guangzhou Liujin Scientific Research Co Ltd
Current assignee: Guangzhou Liujin Scientific Research Co Ltd
Priority date: 2019-10-30
Filing date: 2019-10-30
Publication date: 2019-12-20
Anticipated expiration: 2039-10-30
Also published as: CN110585102B

Abstract

The invention provides a blackhead-derived plant composition, a blackhead-derived liquid and a preparation method thereof. The blackhead-derived botanical composition comprises an aloe vera leaf extract, a pomegranate fruit extract, a tea leaf extract and an agave stem extract. The blackhead eduction liquid comprises the following components in percentage by weight: the blackhead derived plant composition comprises 0.5-10% of the blackhead derived plant composition, 10-40% of a humectant, 0.01-1% of a solubilizer and the balance of water. The blackhead drainage liquid can soften and loosen the keratotic plug by efficiently permeating pores, so that blackheads and whiteheads naturally float out, and simultaneously, the problems of melanogenesis inhibition and grease secretion inhibition are fundamentally solved by deeply dredging the pores.

Description

Blackhead-derived plant composition, blackhead-derived liquid and preparation method

Technical Field

The invention belongs to the technical field of cosmetics, and particularly relates to a blackhead-derived plant composition, a blackhead-derived liquid and a preparation method thereof.

Background

Acne is one of the most common diseases in the skin-care department, easily occurs on the face, is easy to generate scars after improper treatment, and brings trouble to people. Blackhead comedones, also known as blackheads, are open comedones, which are good to the face and back and are characterized by dark spots in the enlarged pores, which appear as small insects after extrusion, with blackened tops. The etiology is as follows: the pilosebaceous canal generates hyperkeratosis hair follicle wall hypertrophy under the action of androgen, the excretion of the sebaceous gland is blocked, and the increase of epithelial cells exfoliated from the hair follicle wall and the excretion of the sebaceous gland are blocked. The mixture of the epithelial cells on the hair follicle wall and the sebum blocks the hair follicle opening to form acne, and the acne is exposed at the top end outside the hair follicle opening. Through the oxidation of air, the deposition of melanin, the pollution of dust and the radiation of ultraviolet rays, blackheads are formed.

The existing solutions in the market for such situations have poor effects, lack basic research on blackhead causes, and are compounded by blindly using some plant ingredients, so that although the solutions have a good moistening effect on skin, the current situation of blackheads cannot be improved, and even more blackheads are used, the more the situation is obvious. The reasons are that they cannot deeply dredge pores, cannot inhibit secretion of sebaceous glands, the root cause of hair follicle obstruction is not solved, comedones can quickly recur, and the phenomenon of proneness occurs. The blackhead removing cosmetics capable of controlling oil are mostly chemical synthetic products, blackheads are removed through violence of a chemical adsorbent, harm to skin is great after the blackhead removing cosmetics are used, although the blackhead removing cosmetics can temporarily solve the blackhead problem, the blackhead removing cosmetics cause great damage and harm to pores, elastic fibers are prone to losing energy, the skin is loose, and the pores are large.

CN108567634A discloses a pure natural blackhead export repair composition and a preparation method thereof, and the composition is prepared from 0.1 to 10 parts by weight of red melon fruit, 0.1 to 10 parts by weight of rambutan and 0.1 to 10 parts by weight of Uncaria tomentosa. Although the blackhead export repair composition can temporarily solve the blackhead problem, the root cause of hair follicle blockage is not solved, and the current situation of blackheads cannot be fundamentally improved.

CN105662914A discloses a blackhead discharge liquid and a blackhead discharge film for reducing stimulation, wherein the blackhead discharge liquid comprises organic acid and chitosan; the blackhead removing film consists of a blackhead removing solution and a chitosan film, the chitosan film is completely soaked in the blackhead removing solution, and the blackhead removing solution contains organic acid as a main effective component. The main effective component in the blackhead removing solution is organic acid, and the blackhead removing solution can cause great damage and harm to pores after long-time use, so that elastic fibers of the skin lose vitality, the skin is loose, and the pores are large.

CN105078796A discloses a blackhead-derived essence film, which adopts traditional Chinese medicinal materials such as radix angelicae, rhizoma typhonii, cortex phellodendri, radix scutellariae and chamomile, and although the blackhead-derived essence film can temporarily solve the blackhead problem, the blackhead-derived essence film has poor capability of inhibiting grease generation, and cannot fundamentally solve the blackhead generation.

Therefore, the development of a care product capable of effectively and gently leading out the blackhead to inhibit the blackhead regeneration is the focus of current research in the field.

Disclosure of Invention

Aiming at the defects of the prior art, the invention aims to provide the blackhead-derived plant composition, the blackhead-derived liquid and the preparation method.

In order to achieve the purpose, the invention adopts the following technical scheme:

in a first aspect, the present invention provides a blackhead-derived botanical composition comprising aloe vera leaf extract, pomegranate fruit extract, tea leaf extract, and agave stem extract.

In the aloe vera leaf extract, the aloe vera leaf extract is mild in nature, is often used as an anti-inflammatory and fire-reducing drug, and has the effects of removing acnes, replenishing water and whitening, and the anthrone compounds contained in the aloe leaves have the effects of sterilizing, inhibiting bacteria, diminishing inflammation, inhibiting bacteria and promoting wound healing, are beneficial to permeating pores, and have the effects of repairing skin and resisting aging by softening and loosening keratotic plugs.

In the invention, the pomegranate fruit extract has the effects of oxidation resistance, inflammation diminishing, bacteria resisting and the like, the effective components are anthocyanin, pomegranate polyphenol and ellagic acid, the pomegranate polyphenol, anthocyanin and ellagic acid are extremely strong oxidation resisting substances, the automatic oxidation of grease is a free radical chain reaction, a plurality of oxygen radicals can be generated in the reaction process, the oxygen radicals have high reaction activity, are combined with macromolecular substances such as lipid, protein and DNA in a human body to damage the structure and the function of the oxygen radicals, and the deposition of hyperkeratosis hair follicle wall and melanin is relieved by relieving the automatic oxidation of the grease, so that the regeneration of blackheads is fundamentally relieved.

In the invention, the tea extract contains superoxide dismutase with higher concentration, can well inhibit the melanin deposition process, avoid the spot precipitation, achieve the whitening effect and fundamentally relieve the blackhead generation. Meanwhile, the tea extract also contains tea polyphenol which can perform chemical reaction with protein, efficiently permeate pores, soften and loosen keratotic plugs, help the convergence of the pores after the blackheads are led out, and prevent the oil from being deposited again after the pores are enlarged. In addition, the tea polyphenol has a moisturizing effect, so that the tea polyphenol can effectively lock the water in human cells, and the excessive dissipation of the water is well avoided.

In the present invention, the agave stem extract has an inhibitory effect on hyaluronidase, which is effective in inhibiting the decomposition of hyaluronic acid during use, thus having a skin moisturizing effect, and in addition, their application can increase the water content of the horny layer, which helps to soften the clogged keratome and open the pores, and in addition, has a superior antiallergic effect, an inhibitory effect on elastase and collagenase activities, and an anti-wrinkle effect.

The four plant extracts are selected to be matched with each other, have synergistic interaction, can permeate pores efficiently, soften and loosen keratotic plugs, enable black heads and white heads to float naturally, and simultaneously dredge the pores deeply, inhibit secretion of sebaceous glands and inhibit generation of melanin so as to achieve the purposes of rapidly converging the pores, fundamentally contacting the problem of hair follicle blockage and have good moistening effect on skin.

Preferably, the blackhead-derived plant composition comprises the following components in parts by weight: 0.01-5 parts of aloe vera leaf extract, 0.01-2 parts of pomegranate fruit extract, 0.01-2 parts of tea leaf extract and 0.01-2 parts of agave stem extract.

In the present invention, the aloe vera leaf extract may be present in an amount of 0.01 to 5 parts by weight, for example, 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5 parts.

In the present invention, the weight part of the pomegranate fruit extract is 0.01 to 2 parts, and may be, for example, 0.01 part, 0.02 part, 0.03 part, 0.04 part, 0.05 part, 0.06 part, 0.07 part, 0.08 part, 0.09 part, 0.1 part, 0.2 part, 0.3 part, 0.4 part, 0.5 part, 0.6 part, 0.7 part, 0.8 part, 0.9 part, 1 part, 1.1 part, 1.2 part, 1.3 part, 1.4 part, 1.5 part, 1.6 part, 1.7 part, 1.8 part, 1.9 part, 2 parts.

In the present invention, the tea extract may be present in an amount of 0.01 to 2 parts by weight, for example, 0.01 part, 0.02 part, 0.03 part, 0.04 part, 0.05 part, 0.06 part, 0.07 part, 0.08 part, 0.09 part, 0.1 part, 0.2 part, 0.3 part, 0.4 part, 0.5 part, 0.6 part, 0.7 part, 0.8 part, 0.9 part, 1 part, 1.1 part, 1.2 part, 1.3 part, 1.4 part, 1.5 part, 1.6 part, 1.7 part, 1.8 part, 1.9 part, 2 part.

In the present invention, the weight parts of the agave stem extract may be 0.01 to 2 parts, for example, 0.01 part, 0.02 part, 0.03 part, 0.04 part, 0.05 part, 0.06 part, 0.07 part, 0.08 part, 0.09 part, 0.1 part, 0.2 part, 0.3 part, 0.4 part, 0.5 part, 0.6 part, 0.7 part, 0.8 part, 0.9 part, 1 part, 1.1 part, 1.2 part, 1.3 part, 1.4 part, 1.5 part, 1.6 part, 1.7 part, 1.8 part, 1.9 part, 2 parts.

In a second aspect, the invention provides a blackhead drainage liquid, which comprises the following components in percentage by weight: the blackhead-derived plant composition according to the first aspect is 0.5-10%, the humectant is 10-40%, the solubilizer is 0.01-1%, and the balance is water.

The blackhead-derived plant composition is reasonably matched with the humectant, the solubilizer and the water to prepare the blackhead-derived liquid, and the blackhead-derived liquid can effectively soften and block keratotic plugs, open pores, promote the emergence of blackheads and whiteheads, also has the effects of shrinking the pores, diminishing inflammation and diminishing swelling, weakening skin injury after the blackheads are removed, enhancing the skin regulation capacity and effectively inhibiting grease secretion and melanin generation, so that the blackhead problem is not easy to relapse, and also has the advantages of moisturizing the skin and enabling the skin to be white and fine. In addition, the sodium lactate with a higher safety index, namely the skin conditioner, is selected from a product of plant fermentation, is attached to the surface of the horny layer, has high-efficiency hygroscopicity, increases the hydration of the horny layer, has high-efficiency moisturizing performance, is antibacterial, can reduce the irritation of a system to the skin, and eliminates the skin injury after blackheads are removed.

In the present invention, the blackhead-derived plant composition according to the first aspect may be present in an amount of 0.5 to 10%, for example, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5%, 5.5%, 6%, 6.5%, 7%, 7.5%, 8%, 8.5%, 9%, 9.5%, 10%.

In the present invention, the content of the humectant is 10 to 30%, and may be, for example, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%.

In the present invention, the content is 0.01 to 1%, and may be, for example, 0.01%, 0.02%, 0.04%, 0.06%, 0.08%, 0.1%, 0.15%, 0.2%, 0.25%, 0.3%, 0.35%, 0.4%, 0.45%, 0.5%, 0.55%, 0.6%, 0.65%, 0.7%, 0.75%, 0.8%, 0.85%, 0.9%, 0.95%, 1%.

Preferably, the humectant is selected from any one or a combination of at least two of propylene glycol, butylene glycol, glycerin, erythritol, lactic acid, or sodium lactate.

Preferably, the humectant is a combination of propylene glycol, butylene glycol, glycerin, erythritol, lactic acid, and sodium lactate.

In the invention, the alcohol humectant is any one or a combination of at least two of propylene glycol, butylene glycol, glycerol or erythritol.

Preferably, the solubilizer is selected from any one or combination of at least two of PEG-20 hydrogenated castor oil, PEG-40 hydrogenated castor oil or PRG-60 hydrogenated castor oil, preferably PEG-40 hydrogenated castor oil.

Preferably, the blackhead eduction liquid also comprises a preservative.

Preferably, the preservative is present in the blackhead eduction liquid in an amount of 0.01 to 1% by weight, for example 0.01%, 0.02%, 0.03%, 0.04%, 0.05%, 0.06%, 0.07%, 0.08%, 0.09%, 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1%.

Preferably, the preservative is selected from any one or combination of at least two of phenoxyethanol, ethylhexyl glycerol, p-hydroxyacetophenone, methyl hydroxybenzoate, propyl hydroxybenzoate or sodium benzoate, preferably phenoxyethanol and/or ethylhexyl glycerol.

Preferably, the blackhead eduction liquid also comprises essence.

Preferably, the weight percentage of the essence in the blackhead eduction liquid is 0.01-0.5%, for example, 0.01%, 0.02%, 0.03%, 0.04%, 0.05%, 0.06%, 0.07%, 0.08%, 0.09%, 0.1%, 0.2%, 0.3%, 0.4%, 0.5%.

Preferably, the essence is selected from any one of or a combination of at least two of rose essence, jasmine essence, cinnamon essence, tangerine essence, ylang-ylang essence, sandalwood essence and sage essence.

Preferably, the blackhead eduction liquid comprises the following components in percentage by weight: the blackhead-derived plant composition according to the first aspect, wherein the blackhead-derived plant composition comprises 0.5-10% of humectant 10-40%, solubilizer 0.01-1%, preservative 0.01-1% and essence 0.01-0.5%, and the balance of water.

Preferably, the blackhead eduction liquid comprises the following components in percentage by weight: the blackhead-derived plant composition according to the first aspect, wherein the blackhead-derived plant composition comprises 0.5-10% of propylene glycol, 1-10% of butylene glycol, 3-10% of glycerol, 1-5% of lactic acid, 0.5-3% of sodium lactate, 0.01-1% of a solubilizer, 0.01-1% of a preservative, 0.01-0.5% of a perfume, and the balance of water.

In a third aspect, the present invention provides a method for preparing blackhead lead-out liquid according to the second aspect, wherein the method comprises the following steps:

(1) mixing the humectant with water, heating and stirring to obtain a water phase mixed solution;

(2) adding the blackhead-derived plant composition and the solubilizer in the first aspect into the aqueous phase mixed solution obtained in the step (1), stirring and mixing, cooling, and filtering to obtain the blackhead-derived liquid.

Preferably, the heating temperature in step (1) is 80-85 deg.C, such as 80 deg.C, 81 deg.C, 82 deg.C, 83 deg.C, 84 deg.C, 85 deg.C.

Preferably, the stirring time in step (1) is 5-10min, such as 5min, 6min, 7min, 8min, 9min, 10 min.

Preferably, the stirring container in the step (1) is a vacuum emulsifying pot.

Preferably, the stirring temperature in step (2) is 45-50 deg.C, such as 45 deg.C, 46 deg.C, 47 deg.C, 48 deg.C, 49 deg.C, 50 deg.C.

Preferably, the cooling temperature in step (2) is 20-38 deg.C, such as 20 deg.C, 22 deg.C, 24 deg.C, 26 deg.C, 28 deg.C, 30 deg.C, 32 deg.C, 34 deg.C, 36 deg.C, 38 deg.C.

Preferably, the filtration in the step (2) is performed by using a filter cloth.

Preferably, the mesh number of the filter cloth is 100-300 meshes, for example, 100 meshes, 120 meshes, 140 meshes, 160 meshes, 180 meshes, 200 meshes, 220 meshes, 240 meshes, 260 meshes, 280 meshes, 300 meshes, preferably 200 meshes.

Preferably, the preparation method comprises the following steps:

(1) mixing humectant with water, heating to 80-85 deg.C, and stirring for 5-10min to obtain water phase mixed solution;

(2) adding the blackhead derived plant composition and the solubilizer in the first aspect into the aqueous phase mixed solution obtained in the step (1), stirring and mixing at 45-50 ℃, cooling to 20-38 ℃, and filtering by using 100-300-mesh filter cloth to obtain the blackhead derived liquid;

preferably, the preparation method comprises the following steps:

(1) mixing alcohol humectant with water, heating to 80-85 deg.C, stirring for 5-10min, adding lactic acid, and stirring at 55-60 deg.C to obtain water phase mixed solution;

(2) adding the blackhead-derived plant composition, sodium lactate, solubilizer, preservative and essence as described in claim 1 or 2 into the water-phase mixed solution obtained in the step (1), stirring and mixing at 45-50 ℃, cooling to 20-38 ℃, and filtering with 100-300-mesh filter cloth to obtain the blackhead-derived liquid.

Compared with the prior art, the invention has the following beneficial effects:

1. the blackhead-derived plant composition provided by the invention selects four plant extracts, namely the aloe barbadensis leaf extract, the pomegranate fruit extract, the tea leaf extract and the agave stem extract, and the four plant extracts are matched with each other to realize synergistic interaction, so that the blackhead can be efficiently derived, pores are deeply dredged, the secretion of sebaceous glands is inhibited, the pores are quickly converged, the problem of hair follicle blockage is fundamentally solved, and the blackhead-derived plant composition has a good moisturizing effect on skin.

2. By adding the moisturizing agent, the solubilizer and the water which are the plant composition for guiding the blackheads into the blackheads, the blackheads guiding liquid can effectively soften and block keratotic plugs, open pores, promote the blackheads and whiteheads to emerge, weaken skin injury after the blackheads are removed, enhance the skin regulating capacity, prevent the blackheads from relapsing, moisten the skin and make the skin white and fine.

Detailed Description

The technical solution of the present invention is further explained by the following embodiments. It should be understood by those skilled in the art that the examples are only for the understanding of the present invention and should not be construed as the specific limitations of the present invention.

Example 1

The embodiment provides a blackhead drainage liquid which comprises the following components in percentage by weight:

the preparation method of the blackhead drainage liquid in this embodiment is as follows:

(1) mixing alcohol humectant with water, heating to 80 deg.C, stirring for 5min, adding lactic acid, and stirring at 55 deg.C to obtain water phase mixed solution;

(2) adding the blackhead-derived plant composition, sodium lactate, solubilizer, preservative and essence into the water-phase mixed solution obtained in the step (1), stirring and mixing at 50 ℃, cooling to 8 ℃, and filtering with 200-mesh filter cloth to obtain the blackhead-derived liquid.

Example 2

(1) mixing alcohol humectant with water, heating to 85 deg.C, stirring for 10min, adding lactic acid, and stirring at 60 deg.C to obtain water phase mixed solution;

(2) adding the blackhead-derived plant composition, sodium lactate, solubilizer, preservative and essence into the water-phase mixed solution obtained in the step (1), stirring and mixing at 45 ℃, cooling to 30 ℃, and filtering with 200-mesh filter cloth to obtain the blackhead-derived liquid.

Example 3

Example 4

Example 5

Example 6

Example 7

Example 8

Example 9

Example 10

Comparative example 1

This comparative example provides a blackhead-derived liquid, which is different from example 1 only in that the blackhead-derived plant composition is not contained, and other components and preparation methods are the same as those of example 1.

Comparative example 2

The present comparative example provides a blackhead-derived liquid, which is different from example 1 only in that the blackhead-derived plant composition does not contain aloe vera leaf extract, the weight percentage of pomegranate fruit extract is 0.31%, the weight percentage of tea leaf extract is 0.3%, the weight percentage of agave stem extract is 0.29%, and other components and preparation methods are the same as example 1.

Comparative example 3

The present comparative example provides a blackhead-derived liquid, which is different from example 1 only in that the blackhead-derived plant composition does not contain pomegranate fruit extract, the weight percentage of aloe vera leaf extract is 0.5%, the weight percentage of tea leaf extract is 0.2%, the weight percentage of agave stem extract is 0.2%, and other components and preparation methods are the same as example 1.

Comparative example 4

The present comparative example provides a blackhead-derived liquid, which is different from example 1 only in that the blackhead-derived plant composition does not contain a tea leaf extract, the weight percentage of the aloe vera leaf extract is 0.5%, the weight percentage of the pomegranate fruit extract is 0.21%, the weight percentage of the agave stem extract is 0.19%, and other components and preparation methods are the same as example 1.

Comparative example 5

The present comparative example provides a blackhead-derived liquid, which is different from example 1 only in that the blackhead-derived plant composition does not contain agave stem extract, the weight percentage of the aloe vera leaf extract is 0.5%, the weight percentage of the pomegranate fruit extract is 0.2%, the weight percentage of the agave stem extract is 0.2%, and other components and preparation methods are the same as example 1.

Test example 1

Safety irritation test

(1) Haemolysis test of erythrocytes

Preparation of erythrocyte suspension: selecting healthy rabbits, taking 9mL of blood from heart, adding 1mL of 2% potassium oxalate solution, centrifuging, discarding supernatant, diluting the precipitate to 20mL with 20mmol/L PBS solution, and storing at 4 ℃ for later use.

The blackhead eduction liquids prepared in examples 1 to 10 and those prepared in comparative examples 1 to 5 were selected and diluted to different concentrations with PBS solution, and 5 concentration gradients were set for each sample. Adding 200 μ L of the above erythrocyte suspension (final concentration of the sample is controlled to be 5, 10, 20, 50, 100mg/mL respectively) into 10mL of diluent of the sample to be tested, taking distilled water as total blood-dissolving control, taking PBS solution as negative control, mixing gently, incubating at 37 deg.C for 30min, centrifuging at 2000r/min for 10min, collecting supernatant, and testing its absorbance at 560nm with spectrophotometer (A)₅₆₀) Calculating the hemolysis rate according to the following formula;

drawingHemolysis rate-sample concentration Standard Curve, the concentration of the sample at which 50% of the erythrocytes were Hemolyzed (HD) was calculated₅₀)。

(2) Protein denaturation experiments:

diluting the blackhead eduction liquid prepared in examples 1-10 and the blackhead eduction liquid prepared in comparative examples 1-5 to 10g/L by PBS solution, taking 10mL of the dilution liquid of a sample to be tested, adding 200 muL of the erythrocyte suspension, taking distilled water as a blank control, taking 1mg/mL of Sodium Dodecyl Sulfate (SDS) solution as a positive control, mixing the solutions gently, incubating the solutions for 30min at 37 ℃, centrifuging the solutions for 10min at the rotating speed of 2000r/min, taking the supernatant, and testing the absorbances A of the supernatant at 540nm and 575nm by a spectrophotometer respectively₅₄₀And A₅₇₅Calculating a protein Denaturation Index (DI) according to the following formula;

wherein R is₁Blank control group a₅₇₅Blank control group A₅₄₀，R₂Experimental group a₅₇₅Experimental group A₅₄₀，R₃Positive control group A₅₇₅Positive control group A₅₄₀。

Evaluating the irritation of the sample to be tested according to the L/D value, wherein the L/D value is HD₅₀DI, erythrocyte hemolysis assay irritation grading criteria are shown in Table 1 below:

TABLE 1

L/D	Grading
		﹥100	Has no irritation
10﹤L/D≤100	Micro-stimulation property
		1﹤L/D≤10	Mild irritation
0.1﹤L/D≤1	Moderate irritation
		L/D≤0.1	Severe irritation

The results of the above-described hemolysis test and protein denaturation test are shown in Table 2 below:

TABLE 2

According to the test results, the irritation of the blackhead drainage liquid prepared in the embodiments 1 to 10 of the invention is classified as no irritation, and the blackhead drainage liquid has the characteristics of being mild and no irritation; the sample concentration HD of 50 percent of red blood cells of the blackhead guide liquid prepared by the invention when hemolysis happens₅₀Above 40000mg/L, is far greater than HD of blackhead eduction liquid prepared in comparative examples 1-5₅₀Meanwhile, the protein denaturation index DI is below 1.00 percent and is also obviously smaller than that of the blackhead eduction liquid prepared in the comparative examples 1-5, which shows that the four plant extracts in the blackhead eduction plant composition are mutually matched, so that the toxic and side effects and the irritation of the blackhead eduction liquid can be obviously reduced after the synergistic effect is achieved, and the blackhead eduction liquid is safer and more reliable.

Test example 2

Blackhead removal effect test

The test method comprises the following steps: 300 volunteers with blackheads on the nose, aged 20-45 years, were randomly divided into 15 groups of 20 persons each. Nonwoven fabric sheets of 5cm × 5cm in the same size were cut out, and the test pieces were used by immersing them in the blackhead drainage liquid prepared in examples 1 to 10 and the blackhead drainage liquid prepared in comparative examples 1 to 5, respectively. The using method comprises the following steps: cleaning and wiping the nose of each group of volunteers, attaching the soaked membrane cloth to the nose, taking off after 10min, cleaning the nose with clear water, sticking the blackheads out with sticky adhesive tape, and observing the blackhead amount on the adhesive tape. The effective standard is that after the adhesive plaster is used, a large number of black heads or white heads are obviously stuck on the adhesive plaster; the standard for judging the effect is that after the adhesive plaster is used, obvious black heads or white heads are stuck on the adhesive plaster; the criterion for judging the ineffectiveness is that after the adhesive tape is used, the adhesive tape has almost no black or white heads, and the number of effective persons, the number of effective persons and the number of ineffective persons in each group are respectively counted, and the specific test results are shown in table 3.

TABLE 3

Test sample	Number of effective persons	The number of persons who take effect	Number of invalid persons
				Example 1	17	3	0
Example 2	17	3	0
				Example 3	19	1	0
Example 4	18	2	0
				Example 5	20	0	0
Example 6	17	3	0
				Example 7	17	3	0
Example 8	16	4	0
				Example 9	16	4	0
Example 10	15	5	0
				Comparative example 1	0	2	18
Comparative example 2	4	6	10
				Comparative example 3	6	3	11
Comparative example 4	3	5	12
				Comparative example 5	6	4	10

From the above test results, it can be seen that the number of people who have significant blackheads or white heads stuck to the adhesive tape using the blackhead drainage liquid prepared in examples 1 to 10 of the present invention was 15 or more, and there was no person who was ineffective after use. The blackhead-derived plant composition is prepared from four plant extracts, namely an aloe vera leaf extract, a pomegranate fruit extract, a tea leaf extract and an agave stem extract, and is matched with each other, synergistic, mild and nonirritating, and can naturally emerge blackheads and whiteheads by efficiently permeating pores, softening and loosening keratotic plugs.

Test example 3

Test for inhibiting melanin synthesis in melanocytes

B16 melanoma cells were cultured at 1X 10⁵one/mL of the cells were seeded in 96-well plates at 90. mu.L/well in CO₂After 24 hours of incubation in the incubator, blackhead catheterizing solution prepared in examples 1 to 10 and blackhead catheterizing solution sample solutions prepared in comparative examples 1 to 5 were added, respectively. A blank control group of medium and cells was set up simultaneously. After the plate was incubated in an incubator for 72 hours, the supernatant was discarded, washed twice with PBS (phosphate buffered saline), and then 0.5mL of trypsinized cells were added to each well for 3min, and 2mL of a maintenance solution was added to each well to terminate the digestion. After mixing, 0.5mL of each concentration was taken out and counted. Centrifuging the rest cell suspension at 2500r/min for 5min, discarding supernatant, adding NaOH solution into the precipitate, heating to dissolve melanin, and measuring absorbance at 490nm under enzyme-linked immunosorbent assay.

The formula for calculating the melanin synthesis inhibition (%) of the sample is shown as follows:

wherein A is₁Is the absorbance value of the drug well, P₁Cell density of drug well, A₂Absorbance values for control wells; p₂In order to control the cell density of the wells, I' is the melanin synthesis inhibition (%) of the corresponding samples, and the specific test results are shown in table 4.

TABLE 4

From the above test results, it can be seen that the blackhead-derived liquid prepared in examples 1 to 10 of the present invention can effectively inhibit melanin synthesis, and the inhibition rate of melanin synthesis is above 50%, while the blackhead-derived liquid prepared in comparative examples 1 to 5 has far lower inhibition effect on melanin synthesis than the blackhead-derived liquid prepared in the present application, which indicates that the blackhead-derived plant composition of the present invention selects four plant extracts, i.e., aloe barbadensis leaf extract, pomegranate fruit extract, tea leaf extract and agave stem extract, and makes them cooperate with each other to achieve synergistic effect, so that not only blackhead and whitehead can be removed, but also melanin can be effectively inhibited from being generated, thereby being helpful to fundamentally solve the blackhead problem and preventing the blackhead from being regenerated again.

Test example 4

Oil control Effect test

Test samples: blackhead drainage liquids prepared in examples 1 to 10 and blackhead drainage liquids prepared in comparative examples 1 to 5. As experimental subjects, 5 male SD rat livers were prepared. The specific test method comprises the following steps: the method comprises the steps of preparing 15 identical 5 alpha-reducing enzyme solutions by using liver homogenates of 5 male SD rats, dividing the 5 alpha-reducing enzyme solutions into 15 groups, adding one of the drainage fluid samples into each group, and detecting the inhibition rate of the oil control composition containing the oil control composition on the 5 alpha-reducing enzyme solutions through a series of reaction systems, wherein the higher the inhibition rate is, the better the oil control effect is. The test results are shown in table 5:

TABLE 5

Sample (I)	5 alpha-reductase inhibition rate
		Example 1	51.15％
Example 2	49.84％
		Example 3	58.06％
Example 4	57.67％
		Example 5	57.15％
Example 6	51.84％
		Example 7	50.84％
Example 8	49.84％
		Example 9	48.84％
Example 10	48.52％
		Comparative example 1	8.45％
Comparative example 2	20.63％
		Comparative example 3	22.35％
Comparative example 4	29.47％
		Comparative example 5	25.48％

From the above test results, it is understood that the blackhead eduction liquids prepared in examples 1 to 10 have higher inhibition ratio of 5 α -reductase liquid than the blackhead eduction liquids prepared in comparative examples 1 to 5, and the inhibition ratio of 5 α -reductase liquid is more than 48%, and the highest inhibition ratio can reach 58.06%. The blackhead-derived plant composition selects four plant extracts, namely the aloe vera leaf extract, the pomegranate fruit extract, the tea leaf extract and the agave stem extract, and the four plant extracts are matched with each other to realize synergistic interaction, so that the blackhead and the white head can be removed, and the generation of grease can be effectively controlled, thereby solving the problems of the obstructed sebaceous gland excretion, the increase of epithelial cells falling off from the hair follicle wall and the obstructed sebaceous gland excretion, solving the root problem generated by the blackhead and fundamentally avoiding the regeneration of the blackhead.

The applicant states that the blackhead-derived plant composition, blackhead-derived liquid and preparation method of the present invention are illustrated by the above examples, but the present invention is not limited to the above examples, i.e., it is not meant that the present invention is necessarily implemented by the above examples. It should be understood by those skilled in the art that any modification of the present invention, equivalent substitutions of the raw materials of the product of the present invention, addition of auxiliary components, selection of specific modes, etc., are within the scope and disclosure of the present invention.

Claims

1. A blackhead-derived botanical composition, comprising an aloe vera leaf extract, a pomegranate fruit extract, a tea leaf extract and an agave stem extract.

2. The blackhead-derived plant composition according to claim 1, comprising in parts by weight: 0.01-5 parts of aloe vera leaf extract, 0.01-2 parts of pomegranate fruit extract, 0.01-2 parts of tea leaf extract and 0.01-2 parts of agave stem extract.

3. The blackhead drainage liquid is characterized by comprising the following components in percentage by weight: the blackhead-derived plant composition according to claim 1 or 2, wherein the blackhead-derived plant composition comprises 0.5 to 10% of humectant, 10 to 40% of solubilizer, 0.01 to 1% of solubilizer, and the balance of water.

4. The blackhead drainage liquid according to claim 3, wherein the humectant is selected from any one or a combination of at least two of propylene glycol, butylene glycol, glycerin, erythritol, lactic acid or sodium lactate.

5. The blackhead drainage liquid according to claim 3 or 4, wherein the humectant is a combination of propylene glycol, butylene glycol, glycerin, erythritol, lactic acid and sodium lactate.

6. The blackhead drainage liquid according to any one of claims 3 to 5, wherein the solubilizer is selected from any one or a combination of at least two of PEG-20 hydrogenated castor oil, PEG-40 hydrogenated castor oil or PRG-60 hydrogenated castor oil, preferably PEG-40 hydrogenated castor oil.

7. The blackhead lead-out liquid according to any one of claims 3 to 6, wherein the blackhead lead-out liquid further comprises a preservative;

preferably, the weight percentage of the preservative in the blackhead eduction liquid is 0.01-1%;

preferably, the preservative is selected from any one or a combination of at least two of phenoxyethanol, ethylhexyl glycerol, p-hydroxyacetophenone, methyl hydroxybenzoate, propyl hydroxybenzoate or sodium benzoate, preferably phenoxyethanol and/or ethylhexyl glycerol;

preferably, the blackhead eduction liquid also comprises essence;

preferably, the weight percentage of the essence in the blackhead eduction liquid is 0.01-0.5%;

preferably, the essence is selected from any one of or a combination of at least two of rose essence, jasmine essence, cinnamon essence, tangerine essence, ylang-ylang essence, sandalwood essence or sage essence;

preferably, the blackhead eduction liquid comprises the following components in percentage by weight: the blackhead-derived plant composition according to claim 1 or 2, wherein the blackhead-derived plant composition comprises 0.5 to 10% of humectant, 10 to 40% of solubilizer, 0.01 to 1% of preservative, 0.01 to 0.5% of essence, and the balance of water.

8. The method for preparing blackhead drainage liquid according to any one of claims 3 to 7, wherein the method comprises the following steps:

(2) adding the blackhead-derived plant composition and the solubilizer as defined in claim 1 or 2 into the aqueous phase mixture obtained in step (1), stirring and mixing, cooling, and filtering to obtain the blackhead-derived liquid.

9. The method according to claim 8, wherein the heating temperature in step (1) is 80-85 ℃;

preferably, the stirring time of the step (1) is 5-10 min;

preferably, the stirred vessel in the step (1) is a vacuum emulsifying pot;

preferably, the stirring temperature of the step (2) is 45-50 ℃;

preferably, the temperature of the cooling in the step (2) is 20-38 ℃;

preferably, the filtration in the step (2) is carried out by using a filter cloth;

preferably, the mesh number of the filter cloth is 100-300 meshes, and preferably 200 meshes.

10. The method according to any one of claims 8 or 9, characterized by comprising the steps of:

(2) adding the blackhead derived plant composition and the solubilizer as defined in claim 1 or 2 into the aqueous phase mixed solution obtained in the step (1), stirring and mixing at 45-50 ℃, cooling to 20-38 ℃, and filtering with 100-300-mesh filter cloth to obtain the blackhead derived solution;

preferably, the preparation method comprises the following steps: