CN110583860A - Fermentation method of feed for tender stem leaves of broussonetia papyrifera - Google Patents
Fermentation method of feed for tender stem leaves of broussonetia papyrifera Download PDFInfo
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- 238000000855 fermentation Methods 0.000 title claims abstract description 78
- 230000004151 fermentation Effects 0.000 title claims abstract description 78
- 241000705930 Broussonetia papyrifera Species 0.000 title claims abstract description 45
- 238000000034 method Methods 0.000 title claims abstract description 23
- 239000000463 material Substances 0.000 claims abstract description 44
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 40
- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 17
- 235000013312 flour Nutrition 0.000 claims abstract description 17
- 235000009566 rice Nutrition 0.000 claims abstract description 17
- 241000933832 Broussonetia Species 0.000 claims abstract description 14
- 240000008042 Zea mays Species 0.000 claims abstract description 13
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 13
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 13
- 235000005822 corn Nutrition 0.000 claims abstract description 13
- 239000012530 fluid Substances 0.000 claims abstract description 9
- 238000007789 sealing Methods 0.000 claims abstract description 9
- 230000004913 activation Effects 0.000 claims abstract description 8
- 238000000227 grinding Methods 0.000 claims abstract description 8
- 230000003213 activating effect Effects 0.000 claims abstract description 7
- 240000007594 Oryza sativa Species 0.000 claims abstract 4
- 239000000203 mixture Substances 0.000 claims description 30
- 239000003795 chemical substances by application Substances 0.000 claims description 11
- 230000015556 catabolic process Effects 0.000 claims description 10
- 238000006731 degradation reaction Methods 0.000 claims description 10
- 238000002156 mixing Methods 0.000 claims description 9
- 239000003755 preservative agent Substances 0.000 claims description 8
- 230000002335 preservative effect Effects 0.000 claims description 8
- 238000005507 spraying Methods 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 239000002390 adhesive tape Substances 0.000 claims description 4
- 238000004140 cleaning Methods 0.000 claims description 4
- 239000000428 dust Substances 0.000 claims description 4
- 239000006260 foam Substances 0.000 claims description 4
- 238000010438 heat treatment Methods 0.000 claims description 3
- 238000005516 engineering process Methods 0.000 claims description 2
- 238000010008 shearing Methods 0.000 claims description 2
- 235000013325 dietary fiber Nutrition 0.000 claims 2
- 230000002906 microbiologic effect Effects 0.000 claims 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 27
- 239000011358 absorbing material Substances 0.000 abstract description 3
- 241000235342 Saccharomycetes Species 0.000 abstract description 2
- 238000002360 preparation method Methods 0.000 abstract 1
- 241000209094 Oryza Species 0.000 description 13
- 239000004033 plastic Substances 0.000 description 4
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 235000019750 Crude protein Nutrition 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
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- 239000011521 glass Substances 0.000 description 2
- 244000144972 livestock Species 0.000 description 2
- 101710130006 Beta-glucanase Proteins 0.000 description 1
- 108010059892 Cellulase Proteins 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 229910021536 Zeolite Inorganic materials 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 239000003674 animal food additive Substances 0.000 description 1
- 229940106157 cellulase Drugs 0.000 description 1
- 235000019784 crude fat Nutrition 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 235000012041 food component Nutrition 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
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- 150000008442 polyphenolic compounds Chemical class 0.000 description 1
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- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 108010083879 xyloglucan endo(1-4)-beta-D-glucanase Proteins 0.000 description 1
- 239000010457 zeolite Substances 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Polymers & Plastics (AREA)
- Biotechnology (AREA)
- Animal Husbandry (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Food Science & Technology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Botany (AREA)
- Mycology (AREA)
- Sustainable Development (AREA)
- Fodder In General (AREA)
Abstract
The invention discloses a fermentation method of a feed for tender stems and leaves of a hybrid paper mulberry, which comprises the steps of selection and treatment of tender stems and leaves, activation of dry yeast, preparation of a fermentation material, laying and fermentation of the fermentation material, sealing treatment and the like. Aiming at the problem that the fermentation material is rotten and goes bad because excessive water is difficult to treat in the fermentation process of the broussonetia papyrifera tender stem leaf feed, the invention provides the broussonetia papyrifera tender stem leaf feed fermentation method which comprises the steps of laying water absorbing materials at intervals, properly increasing the proportion of rice bran and corn flour and activating dry yeast by using the broussonetia papyrifera tender stem leaf grinding fluid. The problem that fermentation materials are rotten and deteriorated due to the fact that the water content is too high in the fermentation process of the broussonetia papyrifera tender stem leaf feeds is solved, the contact intimate degree of the saccharomycetes and the broussonetia papyrifera tender stem leaf tissues is improved, and therefore the success rate of fermentation of the broussonetia papyrifera tender stem leaf feeds is remarkably improved and the quality of the feeds is improved on the one hand of controlling water and preventing the feeds from being rotten and deteriorated.
Description
Technical Field
The invention belongs to the technical field of plant feed fermentation, and particularly relates to a fermentation method of a feed for tender stems and leaves of a hybrid paper mulberry.
Background
The broussonetia papyrifera hybrid is a broussonetia papyrifera hybrid variety cultured by means of space carrying breeding, hybrid breeding and the like by adopting a modern agricultural breeding technology, and has obvious advantages in the aspects of nutrient components, propagation speed, stress resistance and the like compared with the common broussonetia papyrifera. The content of the crude protein of the hybrid broussonetia papyrifera is up to 26.1 percent, and the hybrid broussonetia papyrifera is rich in nutrient components such as amino acid, vitamin, polyphenol substances and the like. In addition, the hybrid paper mulberry has strong adaptability to the environment. Has the characteristics of strong drought resistance, water conservation, cold resistance, barren resistance, saline-alkali resistance, disease and insect pest resistance and the like, and has low planting cost and fast growth property. Therefore, the fermented hybrid paper mulberry tender stem leaves are excellent animal feed and can be produced in large quantities. At present, many attempts are made in the aspect of making the hybrid paper mulberry feed in China, but the problem of rottenness and deterioration caused by overhigh water content in the feed fermentation process is inevitable. The water content of the tender stem leaves of the broussonetia papyrifera is up to more than 75%, water in the tender stem leaves seeps out in the fermentation process, meanwhile, active dry yeast added in the fermentation needs to be activated in advance, and water of the activated yeast is also added into the feed, so that the water in the fermented feed is possibly overproof. In the existing fermentation process of the broussonetia papyrifera, the method of uniformly mixing and stirring corn flour, rice bran and tender stems and leaves of the broussonetia papyrifera is generally adopted to absorb excessive water, so that the water content of a fermentation material can be controlled to be about 60-70%; however, in experiments we have found that the problems are still not solved by using the traditional fermentation material mixing mode. Therefore, the new material mixing mode and the activation method of the activated dry yeast are the key points for solving the problem of water control in the fermentation process.
Disclosure of Invention
The problems to be solved by the invention are as follows: overcomes the problem that the fermented material is rotten and deteriorated due to overhigh water content in the fermentation process of the feed of the tender stem leaves of the broussonetia papyrifera.
In order to solve the technical problem, the invention discloses a fermentation method of a feed for tender stems and leaves of a hybrid broussonetia papyrifera, which comprises the following steps:
(1) selecting and treating tender stems and leaves: taking young leaves and young stems of the hybrid paper mulberry plants, cleaning dust, removing diseased leaves and old leaves, placing in a ventilated and dry sunshine-free place for airing, and then shearing or grinding into small pieces by a machine for later use;
(2) activating dry yeast: grinding or mechanically squeezing a part of the tender stems and leaves treated in the step (1) to obtain tissue fluid for activating yeast, and putting the residual residues back for fermentation; slowly heating the obtained tissue fluid to 25-35 ℃, adding aroma-producing dry yeast, stirring for 5-10 minutes, covering a preservative film, and standing until foam is formed, namely, completing activation;
(3) preparing a fermentation material: mixing rice bran and corn flour, and dividing into a mixture A and a mixture B; fully and uniformly mixing the tender stems and leaves treated in the step (1), the coarse feed degradation agent and the mixture B to obtain a fermented material;
(4) laying and fermenting a fermentation material: laying a layer of mixture A at the bottom of a fermentation barrel, adding a proper amount of fermentation material into the barrel, compacting, and uniformly spraying the activated dry yeast solution obtained in the step (2); then laying a layer of mixture A, adding a proper amount of fermentation material into the barrel, compacting, and uniformly spraying the activated dry yeast solution obtained in the step (2); and repeating the steps until the top layer of the fermented material is laid, and scattering all the residual dry yeast solution in the top layer of the fermented material;
(5) sealing treatment: covering a layer of preservative film on the surface of the fermentation barrel, compacting by using a heavy object, covering the barrel cover, sealing the edge of the barrel cover by using an adhesive tape, and fermenting for 20-35 days at the temperature of 30-40 ℃ to obtain the feed for the tender stem leaves of the hybrid broussonetia papyrifera.
Preferably, in the step (2), the adding amount of the aroma-producing dry yeast is 18-25 g/40-50ml of tissue fluid; the aroma-producing yeast is aroma-producing active dry yeast produced by Angel Yeast Co.
Specifically, in the step (3), the dosage ratio of the treated tender stem leaves, the coarse feed degradation agent, the rice bran and the corn flour is (250-350 g), (80-120 g) and (10-15 g); preferably 300g:100g:100g:12 g.
Preferably, after being mixed, the rice bran and the corn flour are divided into a mixture A and a mixture B according to the mass ratio of 3: 2; the mixture A has the function of absorbing moisture, and the mixture B mainly has the function of providing energy for fermentation and also has the function of absorbing moisture.
Preferably, in the step (4), the thickness of each layer of the mixture A is 0.4-0.6 cm; the laying thickness of each layer of fermentation material is 3-4 cm.
Preferably, in step (1), the hybrid broussonetia papyrifera plant is at least 2 years old.
Preferably, in the step (1), the size of the crushed small pieces is (2-3) cm x (2-3) cm.
Preferably, the coarse feed degradation agent is a reinforced coarse feed degradation agent produced by Nanning micro-Ray biotechnology limited, the main raw materials are cellulase, beta-glucanase, xyloglucanase and the like, and the auxiliary materials are zeolite powder and the like.
Aiming at the problem that the fermentation material is rotten and goes bad because excessive water is difficult to treat in the fermentation process of the broussonetia papyrifera tender stem leaf feed, the invention provides the broussonetia papyrifera tender stem leaf feed fermentation method which comprises the steps of laying water absorbing materials at intervals, properly increasing the proportion of rice bran and corn flour and activating dry yeast by using the broussonetia papyrifera tender stem leaf grinding fluid. The problem that fermentation materials are rotten and deteriorated due to the fact that the water content is too high in the fermentation process of the broussonetia papyrifera tender stem leaf feeds is solved, the contact intimate degree of the saccharomycetes and the broussonetia papyrifera tender stem leaf tissues is improved, and therefore the success rate of fermentation of the broussonetia papyrifera tender stem leaf feeds is remarkably improved and the quality of the feeds is improved on the one hand of controlling water and preventing the feeds from being rotten and deteriorated.
Has the advantages that:
the hybrid broussonetia papyrifera is rich in various nutrient elements and crude protein, is an ideal animal feed, has high survival rate and strong stress resistance, and is suitable for large-area planting. However, in the fermentation process of the feed of the tender stem leaves of the broussonetia papyrifera, the problem of fermentation failure caused by excessive water is easy to occur. The invention can obviously control the content of water in the fermentation process, and improve the fermentation success rate and the quality of the feed. The water content in the feed of the tender stem leaves of the broussonetia papyrifera fermented by the method is reduced to 46.8 percent, which is superior to the water content of more than 60 percent in the feed of the tender stem leaves of the broussonetia papyrifera common in the market, so that the successful fermentation probability of the feed of the tender stem leaves of the broussonetia papyrifera is greatly improved, and an idea is provided for the large-scale production of the fermented feed of the tender stem leaves of the broussonetia papyrifera.
Detailed Description
The invention will be better understood from the following examples.
Examples
Selecting and treating tender stem leaves
In the sunny morning, young leaves and young stems of strong 2-year-old hybrid paper mulberry plants are taken, cleaned with dust, and the diseased and old leaves are removed, and then the hybrid paper mulberry plants are placed in a ventilated and dry sunshine-free place for airing, and then are cut into pieces or ground into pieces of 2 cm multiplied by 2 cm by a machine. 300g was weighed out and fermented. Grinding or mechanically squeezing a part of the weighed tender stem leaves to obtain 40-50ml of tissue fluid for activating yeast, and returning the residual residues for fermentation.
② the selection and dosage of the fermentation materials, see table 1
TABLE 1
| Serial number | Composition (I) | Dosage (g) | Serial number | Composition (I) | Dosage (g) |
| 1 | Stem and leaf of hybrid paper mulberry | 300 | 4 | Coarse fodder degradation agent | 12 |
| 2 | Corn flour | 100 | 5 | Aroma-producing yeast | 20 |
| 3 | Rice bran | 100 |
Note: wherein the aroma-producing yeast is aroma-producing active dry yeast produced by Angel Yeast Co
Activation of Dry Yeast
Slowly heating the grinding liquid of the tender stem leaves of the broussonetia papyrifera to 30 ℃ in a beaker, pouring the aroma-producing dry yeast into the beaker, stirring for 5-10 minutes by using a glass rod, covering a preservative film, standing for about 5 minutes, and observing that foam is formed, namely the activation is finished.
Selection of fermentation vessels
Selecting a plastic barrel with the diameter of about 20 cm and no peculiar smell, and cleaning and wiping the plastic barrel in advance.
Fifthly, preparing the fermentation material
Firstly, the weighed rice bran and corn flour are mixed and divided into two parts according to the mass ratio of 3:2, namely rice bran-corn flour mixtures A and B, wherein the mixture A has the function of absorbing water, and the mixture B has the main function of providing energy for fermentation and has the function of absorbing water. And fully and uniformly mixing the treated tender stems and leaves, the coarse feed additive and the rice bran-corn flour mixture B to obtain the fermented material.
Sixthly, paving water absorbing material and adding microzyme
Laying a layer of rice bran-corn flour mixture A with the thickness of 0.5 cm on the bottom of a fermentation barrel, adding a proper amount of fermentation material into the barrel, compacting, and keeping the height of the compacted mixture to be about 4 cm; uniformly spraying a proper amount of activated yeast on the activated yeast, paving a layer of rice bran-corn flour mixture A with the height of about 0.5 cm, adding a proper amount of fermentation material into a barrel, compacting, and keeping the height of the compacted mixture to be about 4 cm; and repeating the steps until the hybrid fermentation material is used up, wherein the uppermost layer is a tender stem and leaf fermentation material layer, and redundant microzyme is added into the tender stem and leaf fermentation material layer on the uppermost layer.
Seventh, sealing treatment
Compacting the fermented material in the barrel, covering a layer of preservative film on the surface, compacting by using a weight, covering the barrel cover, sealing the edge of the barrel cover by using an adhesive tape, and placing the barrel cover in an environment at 35 ℃ for fermentation.
Comparative example
Selecting and treating tender stem leaves
In the sunny morning, young leaves and young stems of strong 2-year-old hybrid paper mulberry plants are taken, cleaned with dust, and the diseased and old leaves are removed, and then the hybrid paper mulberry plants are placed in a ventilated and dry sunshine-free place for airing, and then are cut into pieces or ground into pieces of 2 cm multiplied by 2 cm by a machine. 300g was weighed out and fermented.
② the selection and dosage of the fermentation materials, see Table 2
TABLE 2
| Serial number | Composition (I) | Dosage (g) | Serial number | Composition (I) | Dosage (g) |
| 1 | Stem and leaf of hybrid paper mulberry | 300 | 4 | Coarse fodder degradation agent | 12 |
| 2 | Corn flour | 100 | 5 | Aroma-producing yeast | 20 |
| 3 | Rice bran | 100 |
Note: wherein the aroma-producing yeast is aroma-producing active dry yeast produced by Angel Yeast Co
Activation of Dry Yeast
Taking 40-50ml of warm water at about 30 ℃, pouring the aroma-producing dry yeast into a beaker, stirring for 5-10 minutes by using a glass rod, covering a preservative film, standing for about 5 minutes, and observing that foam is formed, namely the activation is finished.
Selection of fermentation vessels
Selecting a plastic barrel with the diameter of about 20 cm and no peculiar smell, and cleaning and wiping the plastic barrel in advance.
Fifthly, preparing the fermentation material
Mixing the cut or ground stem and leaf of Broussonetia papyrifera with the rice bran, corn flour and coarse fodder degrading agent, and preparing into fermented material.
Filling barrel of fermented material and adding yeast
Pouring the prepared fermented material into a barrel, slowly pouring the fermented yeast into the barrel, and compacting the fermented material after the yeast is completely infiltrated.
Seventh, sealing treatment
Compacting the fermented material in the barrel, covering a layer of preservative film on the surface, compacting by using a weight, covering the barrel cover, sealing the edge of the barrel cover by using an adhesive tape, and placing the barrel cover in an environment at 35 ℃ for fermentation.
The fermentation was conducted according to the methods of examples and comparative examples, and the nutritional components and various indexes of the fermented feed were tested after 25 days, and the results are shown in Table 3.
TABLE 3
The nutrition detection shows that the water content in the feed of the tender stem leaves of the broussonetia papyrifera fermented by the fermentation method is remarkably reduced by 24.5 percent, and the fermented material is effectively prevented from being rotten and deteriorated. In addition, the content of crude fiber and crude fat in the fermented feed is reduced, and the fermented feed can promote the digestion of livestock on the feed and promote the healthy growth of meat livestock.
The invention provides a thought and a method for a fermentation method of a hybrid paper mulberry tender stem leaf feed, and a method and a way for realizing the technical scheme are many, the above description is only a preferred embodiment of the invention, and it should be noted that, for a person skilled in the art, a plurality of improvements and decorations can be made without departing from the principle of the invention, and the improvements and decorations should be regarded as the protection scope of the invention. All the components not specified in the present embodiment can be realized by the prior art.
Claims (8)
1. A fermentation method of a feed for tender stems and leaves of a broussonetia papyrifera is characterized by comprising the following steps:
(1) selecting and treating tender stems and leaves: taking young leaves and young stems of the hybrid paper mulberry plants, cleaning dust, removing diseased leaves and old leaves, placing in a ventilated and dry sunshine-free place for airing, and then shearing or grinding into small pieces by a machine for later use;
(2) activating dry yeast: grinding or mechanically squeezing a part of the tender stems and leaves treated in the step (1) to obtain tissue fluid for activating yeast, and putting the residual residues back for fermentation; slowly heating the obtained tissue fluid to 25-35 ℃, adding aroma-producing dry yeast, stirring for 5-10 minutes, covering a preservative film, and standing until foam is formed, namely, completing activation;
(3) preparing a fermentation material: mixing rice bran and corn flour, and dividing into a mixture A and a mixture B; fully and uniformly mixing the tender stems and leaves treated in the step (1), the coarse feed degradation agent and the mixture B to obtain a fermented material;
(4) laying and fermenting a fermentation material: laying a layer of mixture A at the bottom of a fermentation barrel, adding a proper amount of fermentation material into the barrel, compacting, and uniformly spraying the activated dry yeast solution obtained in the step (2); then laying a layer of mixture A, adding a proper amount of fermentation material into the barrel, compacting, and uniformly spraying the activated dry yeast solution obtained in the step (2); and repeating the steps until the top layer of the fermented material is laid, and scattering all the residual dry yeast solution in the top layer of the fermented material;
(5) sealing treatment: covering a layer of preservative film on the surface of the fermentation barrel, compacting by using a heavy object, covering the barrel cover, sealing the edge of the barrel cover by using an adhesive tape, and fermenting for 20-35 days at the temperature of 30-40 ℃ to obtain the feed for the tender stem leaves of the hybrid broussonetia papyrifera.
2. The fermentation method of the Broussonetia papyrifera tender stem leaf feed as claimed in claim 1, wherein in the step (2), the addition amount of the aroma-producing dry yeast is 18-25 g/40-50ml of tissue fluid.
3. The fermentation method of the young stem and leaf feed of the broussonetia papyrifera as claimed in claim 1, wherein in the step (3), the amount ratio of the processed young stem and leaf, the coarse feed degradation agent, the rice bran and the corn flour is (250-350 g), (80-120 g) and (10-15 g).
4. The fermentation method of the feed for the tender stems and leaves of the broussonetia papyrifera as claimed in claim 3, wherein the rice bran and the corn flour are mixed and then divided into a mixture A and a mixture B according to a mass ratio of 3: 2.
5. The fermentation method of the broussonetia papyrifera tender stem leaf feed as claimed in claim 1, wherein in the step (4), the thickness of each layer of the mixture A is 0.4-0.6 cm; the laying thickness of each layer of fermentation material is 3-4 cm.
6. The fermentation method of the Broussonetia papyrifera tender stem leaf feed as claimed in claim 1, wherein in the step (1), the Broussonetia papyrifera plants are at least 2 years old.
7. The fermentation method of the young stem and leaf feed of the broussonetia papyrifera as claimed in claim 1, wherein in the step (1), the size of the crushed pieces is (2-3) cm x (2-3) cm.
8. The fermentation method of the feed of the tender stem leaves of the broussonetia papyrifera as claimed in claim 3, wherein the roughage degradation agent is a reinforced roughage degradation agent produced by Nanning microbiological science and technology Limited.
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|---|---|---|---|---|
| CN103947827A (en) * | 2014-05-16 | 2014-07-30 | 丁友昉 | Fermentation agent and preparation method of probiotics and straw fermented feed |
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Application publication date: 20191220 |