CN110476728B - Cordyceps sinensis strain collection device and method - Google Patents
Cordyceps sinensis strain collection device and method Download PDFInfo
- Publication number
- CN110476728B CN110476728B CN201910738541.7A CN201910738541A CN110476728B CN 110476728 B CN110476728 B CN 110476728B CN 201910738541 A CN201910738541 A CN 201910738541A CN 110476728 B CN110476728 B CN 110476728B
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- collecting box
- isolator
- pipeline
- cordyceps sinensis
- soil
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- 241001248610 Ophiocordyceps sinensis Species 0.000 title claims abstract description 45
- 238000000034 method Methods 0.000 title claims abstract description 15
- 239000011159 matrix material Substances 0.000 claims abstract description 26
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 22
- 239000007921 spray Substances 0.000 claims abstract description 12
- 230000000149 penetrating effect Effects 0.000 claims abstract description 4
- 239000002689 soil Substances 0.000 claims description 57
- 239000000758 substrate Substances 0.000 claims description 25
- 241000330899 Hepialus Species 0.000 claims description 16
- 241000190633 Cordyceps Species 0.000 claims description 12
- 244000060011 Cocos nucifera Species 0.000 claims description 10
- 235000013162 Cocos nucifera Nutrition 0.000 claims description 10
- 230000001954 sterilising effect Effects 0.000 claims description 5
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 239000000741 silica gel Substances 0.000 claims description 3
- 229910002027 silica gel Inorganic materials 0.000 claims description 3
- 241000233866 Fungi Species 0.000 abstract 1
- 241000894006 Bacteria Species 0.000 description 7
- 241000238631 Hexapoda Species 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 3
- 238000005553 drilling Methods 0.000 description 2
- 230000002349 favourable effect Effects 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 239000012212 insulator Substances 0.000 description 2
- 244000025254 Cannabis sativa Species 0.000 description 1
- 230000023753 dehiscence Effects 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 239000005413 snowmelt Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/70—Harvesting
Abstract
The invention discloses a cordyceps sinensis strain collection device and a cordyceps sinensis strain collection method, wherein the device comprises a water collection tank, a pipeline, a water seepage pipe, a collection box, an isolator, an growth hole and a spray header; the water collecting tank is positioned above the collecting box and connected with a pipeline, and the pipeline is respectively led to the upper part and the side surface of the collecting box; the collecting box is of a cuboid structure with a normally open top; the isolator is positioned in the middle part in the collecting box, a plurality of growing holes penetrating through the isolator are formed in the isolator, and the bottom of the collecting box is communicated with the upper space of the isolator through the growing holes; one end of the pipeline, which is led to the upper part of the collecting box, is connected with a spray header; one end of the pipeline, which is led to the side surface of the collecting box, is connected with 2 seepage pipes, and the 2 seepage pipes extend to the lower part of the isolator in the collecting box; the device provides that cordyceps sinensis infects bat moth larva and creates the advantage and cultivates more cordyceps sinensis, sets up the isolator and keeps apart cordyceps sinensis and other miscellaneous fungus, has improved the success rate that cordyceps sinensis infects bat moth larva, has realized the humidity automatic control of matrix.
Description
Technical Field
The invention relates to the technical field of cordyceps sinensis strain collection, in particular to a cordyceps sinensis strain collection device and a collection method using the device.
Background
Hepialus is the main host insect of Cordyceps sinensis, and the life habit of adults mainly breeds the offspring through mating, spawning and other reproductive activities. The hepialus life cycle is as long as 4 years or more than 4 years, the larva period is as long as more than 3 years, the adult period is shorter, about 25-28 days, and the individual survival period is generally 3-4 days. Cordyceps sinensis invades host hepialus larva in 7 and 8 months each year, and the nutrition of the larva is fully utilized to reproduce hyphae so as to fill the whole body cavity. The infected larvae are drilled into the soil for overwintering for about 10 months, and the larvae die to become stiff insects when the ground temperature is 2-9 ℃, and the stiffness degree is higher as the ground temperature is continuously reduced. At the dehiscence line of the stiff larva head, a sub-seat starts to grow. The growth of the sub-base is very slow after the earth temperature is in the range of 1.8-2.2 ℃ from 11 months to 2 months next year. When the temperature rises to 5.5 ℃ in the late 4 months, the stroma grows rapidly. At the beginning of 5 months, the ice and snow melt, the soil layer is thawed, and the sub-base drills out surface soil. The seed bed which is unearthed grows fastest a few days before, and the seed bed is brown in color for 10-20 days, and the top begins to expand to produce ascus fruits. After 25 days the stroma stopped growing. After 48 days, the linear spores are ripe, the underground insects decay, and the stroma begins to wilt. The upper part of the cordyceps fungus sporophore expands into a cylindrical sub-seat, the lower part of the cordyceps fungus sporophore is connected with the head of the cordyceps fungus sporophore through a sub-seat handle, a plurality of ascus shells are arranged in the sub-seat, ascus are arranged in the ascus shells, each ascus contains 2-8 ascospores, and the sub-seat which is uneaten of creates favorable conditions for the propagation and invasion of ascospores to insects;
in the artificial cultivation process of the Cordyceps sinensis, in order to create favorable conditions for the infection of the hepialus larva by the Cordyceps sinensis, a large amount of Cordyceps sinensis is required to be collected, but other miscellaneous bacteria are easy to collect in the traditional collection process, so that the hepialus larva is infected by the miscellaneous bacteria, even mildews occur, and the success rate of cultivating the Cordyceps sinensis is reduced.
Disclosure of Invention
The invention provides a cordyceps sinensis strain collection device and a collection method using the device, which solve the problems that other miscellaneous bacteria are easy to collect in the collection process, so that hepialus larvae are infected by the miscellaneous bacteria, even mildewing occurs, and the success rate of cultivating cordyceps sinensis is low.
In order to achieve the purpose of the invention, the invention adopts the following technical scheme:
the Cordyceps strain collecting device comprises a water collecting tank, a pipeline, a water seepage pipe, a collecting box, an isolator, an growth hole and a spray header; the water collecting tank is positioned above the collecting box and connected with a pipeline, and the pipeline is respectively led to the upper part and the side surface of the collecting box; the collecting box is of a cuboid structure with a normally open top; the separator is positioned in the middle part in the collecting box, and a plurality of growing holes penetrating through the separator are formed in the separator, and the bottom of the collecting box is communicated with the upper space of the separator through the growing holes; one end of the pipeline, which is led to the upper part of the collecting box, is connected with a spray header; one end of the pipeline, which is led to the side surface of the collecting box, is connected with 2 seepage pipes, and the 2 seepage pipes extend into the lower part of the isolator in the collecting box.
Preferably, the cordyceps sinensis strain collecting device further comprises a control circuit, an electromagnetic valve A, an electromagnetic valve B, a soil humidity sensor A and a soil humidity sensor B; the electromagnetic valve A is arranged at the part of the pipeline which is led to the upper part of the collecting box; the electromagnetic valve B is arranged at the part of the pipeline which is communicated with the side surface of the collecting box; the soil humidity sensor A extends below the isolator in the collecting box; the soil humidity sensor B is arranged above the isolator in the collecting box; the control circuit is connected with the electromagnetic valve A, the electromagnetic valve B, the soil humidity sensor A and the soil humidity sensor B.
Preferably, the separator is a sheet structure made of silica gel material; the growth holes are funnel-shaped.
Preferably, the control circuit comprises a singlechip and a switching power supply; the switching power supply is connected with the AV220V, and outputs 5V and 24V for supplying power to the control circuit; the soil humidity sensor A and the soil humidity sensor B are connected with the P30 end and the P31 end of the singlechip through MAX485 chips; the electromagnetic valve A and the electromagnetic valve B are respectively connected with the P10 end and the P11 end of the singlechip through the relay KA.
A method for collecting Cordyceps strain by Cordyceps strain collecting device comprises:
s1: uniformly mixing coconut coir, turfy soil, plateau soil and cordyceps fungus soil to prepare a matrix A, and paving the matrix A at the bottom of the collecting box; putting hepialus larva into a substrate A, and covering an isolator above the substrate A;
s2: uniformly mixing coconut coir, turfy soil and plateau soil, sterilizing at 120deg.C for 30min to obtain matrix B, and laying above the isolator;
s3: the humidity of the matrix A and the humidity of the matrix B are controlled to be 40% -70% by a control circuit;
s4: after the sub-mount grows out of the growth well, it is collected together with the substrate B.
Compared with the prior art, the invention has the following beneficial effects:
the device provides beneficial conditions for the cordyceps sinensis to infect the hepialus larva to culture more cordyceps sinensis, and the isolator is arranged to isolate the cordyceps sinensis from other miscellaneous bacteria, so that the success rate of the cordyceps sinensis to infect the hepialus larva is improved, the grass forming rate of the cordyceps sinensis is higher, and the deterioration of the hepialus larva after being infected by other miscellaneous bacteria in the cordyceps sinensis cultivation process is avoided; through setting up control circuit, realized the humidity automatic control of matrix, reduce the manpower, relative artificial humidification, its control accuracy is higher.
Drawings
FIG. 1 is a schematic diagram of a Cordyceps sinensis strain collection device;
FIG. 2 is a schematic diagram of the structure of a collection box and an isolator in a Cordyceps sinensis strain collection device;
FIG. 3 is a schematic diagram of the structure of an isolator in a Cordyceps sinensis strain collection device;
FIG. 4 is a schematic diagram showing the related structure of a water seepage pipe and a spray header in the cordyceps sinensis strain collection device
FIG. 5 is a control circuit of a Cordyceps sinensis seed collection device;
in the figure: the water collection tank 1, the pipeline 2, the water seepage pipe 21, the collection box 3, the isolator 4, the growth hole 41, the spray header 5, the control circuit 6, the singlechip 61, the switching power supply 62, the electromagnetic valve A7, the electromagnetic valve B8, the soil humidity sensor A9 and the soil humidity sensor B10.
Detailed Description
The drawings in the embodiments of the present invention will be combined; the technical scheme in the embodiment of the invention is clearly and completely described:
as shown in fig. 1-4, a cordyceps sinensis strain collecting device comprises a water collecting tank 1, a pipeline 2, a water seepage pipe 21, a collecting box 3, an isolator 4, a growing hole 41 and a spray header 5; the water collection tank 1 is positioned above the collection box 3, the water collection tank 1 is connected with the pipeline 2, and the pipeline 2 is respectively led to the upper part and the side surface of the collection box 3; the collecting box 3 is of a cuboid structure with a normally open top; the separator 4 is positioned in the middle of the inside of the collecting box 3, a plurality of growing holes 41 penetrating through the separator 4 are formed in the separator 4, and the bottom of the collecting box 3 is communicated with the upper space of the separator 4 through the growing holes; one end of the pipeline 2, which is led to the upper part of the collecting box 3, is connected with a spray header 5, and can spray water to the collecting box 3; one end of the pipeline 2, which is led to the side surface of the collecting box 3, is connected with 2 seepage pipes 21, and the 2 seepage pipes 21 extend to the lower part of the isolator 4 in the collecting box 3; in specific implementation, the substrate easy to grow Cordyceps is arranged above and below the isolator 4 in the collecting box 3, and water is supplied above and below the isolator 4 in the collecting box 3 through the spray header 5 and the water seepage pipe 21, so that the substrate is ensured to be in a certain humidity. And placing hepialus larva and strain soil with cordyceps sinensis into a mechanism below, growing a sub-seat after the hepialus larva is infected by the cordyceps sinensis, drilling the sub-seat into a matrix above the isolator 4 through an growth hole 41 of the isolator 4, and finally collecting the matrix above the isolator 4 together with the grown sub-seat to obtain strain soil for the next infection of cordyceps sinensis.
The cordyceps sinensis strain collection device also comprises a control circuit 6, an electromagnetic valve A7, an electromagnetic valve B8, a soil humidity sensor A9 and a soil humidity sensor B10; the electromagnetic valve A7 is arranged at the part of the pipeline 2 which is led to the upper part of the collecting box 3, and controls the water supply of the substrate above the isolator 4 in the collecting box 3; the electromagnetic valve B8 is arranged at the part of the pipeline 2 leading to the side surface of the collecting box 3 and controls the substrate water supply below the isolator 4 in the collecting box 3; the soil humidity sensor A9 extends into the collecting box 3 below the isolator 4; for collecting the humidity of the matrix under the insulator 4 inside the collection box 3; the soil humidity sensor B10 is arranged above the isolator 4 in the collection box 3; for collecting the humidity of the matrix above the insulator 4 inside the collection box 3; the control circuit 6 is connected with the electromagnetic valve A7, the electromagnetic valve B8, the soil humidity sensor A9 and the soil humidity sensor B10; the control circuit 6 controls the solenoid valve B8 and the solenoid valve A7 to switch the water supply pipeline 2 through collecting the humidity of the soil humidity sensor A9 and the soil humidity sensor B10, and controls the humidity of the matrix in the collecting box 3 within a reasonable range.
Preferably, the isolator 4 is in a sheet structure made of silica gel material, has certain elasticity, and is beneficial to drilling of the sub-base; the growth holes 4 are funnel-shaped, the lower openings are small, and the upper openings are large, so that the mixed bacteria are effectively isolated.
As shown in fig. 5, the control circuit 6 includes a single chip microcomputer 61 (STC 89C52 RC) and a switching power supply 62; the switching power supply 62 is connected with AV220V, and outputs 5V and 24V for supplying power to the control circuit; the soil humidity sensor A9 (CSF 11) and the soil humidity sensor B10 (CSF 11) are connected with the P30 and P31 ends of the singlechip 61 through MAX485 chips, and the specific soil humidity sensor A9 and the soil humidity sensor B10 are communicated with the UART interface of the singlechip 61 through 485 buses; the electromagnetic valve A7 and the electromagnetic valve B8 are respectively connected with the P10 end and the P11 end of the singlechip 61 through the relay KA; specifically, the singlechip 61 controls the opening and closing of the solenoid valve A7 and the solenoid valve B8 through the relay KA.
A first embodiment of a method for collecting cordyceps sinensis strains using a cordyceps sinensis strain collecting device as described above:
s1: uniformly mixing coconut coir, turfy soil, plateau soil and cordyceps fungus soil to prepare a matrix A, and paving the matrix A at the bottom of the collecting box 3; putting hepialus larva into a substrate A, and covering an isolator 4 above the substrate A;
s2: uniformly mixing coconut coir, peatmoss and plateau soil, sterilizing at 120deg.C for 30min to obtain matrix B, and laying above the isolator 4;
s3: the humidity of the substrate A and the substrate B is controlled to be 40% by the control circuit 6;
s4: after the sub-mount grows out of the growth well 41, it is collected together with the substrate B.
A first embodiment of a method for collecting cordyceps sinensis strains using a cordyceps sinensis strain collecting device as described above:
s1: uniformly mixing coconut coir, turfy soil, plateau soil and cordyceps fungus soil to prepare a matrix A, and paving the matrix A at the bottom of the collecting box 3; putting hepialus larva into a substrate A, and covering an isolator 4 above the substrate A;
s2: uniformly mixing coconut coir, peatmoss and plateau soil, sterilizing at 120deg.C for 30min to obtain matrix B, and laying above the isolator 4;
s3: the humidity of the substrate A and the substrate B is controlled to be 60% by the control circuit 6;
s4: after the sub-mount grows out of the growth well 41, it is collected together with the substrate B.
A second embodiment of the method for collecting Cordyceps sinensis strains using the above Cordyceps sinensis strain collecting device:
s1: uniformly mixing coconut coir, turfy soil, plateau soil and cordyceps fungus soil to prepare a matrix A, and paving the matrix A at the bottom of the collecting box 3; putting hepialus larva into a substrate A, and covering an isolator 4 above the substrate A;
s2: uniformly mixing coconut coir, peatmoss and plateau soil, sterilizing at 120deg.C for 30min to obtain matrix B, and laying above the isolator 4;
s3: the humidity of the substrate A and the substrate B is controlled to 70% by the control circuit 6;
s4: after the sub-mount grows out of the growth well 41, it is collected together with the substrate B.
Claims (1)
1. A cordyceps sinensis strain collection device, comprising: the device comprises a water collection tank, a pipeline, a water seepage pipe, a collection box, an isolator, an growth hole and a spray header; the method is characterized in that: the water collecting tank is positioned above the collecting box and connected with a pipeline, and the pipeline is respectively led to the upper part and the side surface of the collecting box; the collecting box is of a cuboid structure with a normally open top; the separator is positioned in the middle part in the collecting box, and a plurality of growing holes penetrating through the separator are formed in the separator, and the bottom of the collecting box is communicated with the upper space of the separator through the growing holes; one end of the pipeline, which is led to the upper part of the collecting box, is connected with a spray header; one end of the pipeline, which is led to the side surface of the collecting box, is connected with 2 seepage pipes, and the 2 seepage pipes extend to the lower part of the isolator in the collecting box; the isolator adopts a sheet structure made of silica gel material; the growth holes are funnel-shaped; the cordyceps sinensis strain collection device also comprises a control circuit, an electromagnetic valve A, an electromagnetic valve B, a soil humidity sensor A and a soil humidity sensor B; the electromagnetic valve A is arranged at the part of the pipeline which is led to the upper part of the collecting box; the electromagnetic valve B is arranged at the part of the pipeline which is communicated with the side surface of the collecting box; the soil humidity sensor A extends below the isolator in the collecting box; the soil humidity sensor B is arranged above the isolator in the collecting box; the control circuit is connected with the electromagnetic valve A, the electromagnetic valve B, the soil humidity sensor A and the soil humidity sensor B; the control circuit comprises a singlechip and a switching power supply; the switching power supply is connected with the AV220V, and outputs 5V and 24V for supplying power to the control circuit; the soil humidity sensor A and the soil humidity sensor B are connected with the P30 end and the P31 end of the singlechip through MAX485 chips; the electromagnetic valve A and the electromagnetic valve B are respectively connected with the P10 end and the P11 end of the singlechip through the relay KA;
the method for collecting cordyceps sinensis strains by using the cordyceps sinensis strain collecting device comprises the following steps of S1: uniformly mixing coconut coir, turfy soil, plateau soil and cordyceps fungus soil to prepare a matrix A, and paving the matrix A at the bottom of the collecting box; putting hepialus larva into a substrate A, and covering an isolator above the substrate A; s2: uniformly mixing coconut coir, turfy soil and plateau soil, sterilizing at 120deg.C for 30min to obtain matrix B, and laying above the isolator; s3: the humidity of the matrix A and the humidity of the matrix B are controlled to be 40% -70% by a control circuit; s4: after the sub-mount grows out of the growth well, it is collected together with the substrate B.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910738541.7A CN110476728B (en) | 2019-08-12 | 2019-08-12 | Cordyceps sinensis strain collection device and method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910738541.7A CN110476728B (en) | 2019-08-12 | 2019-08-12 | Cordyceps sinensis strain collection device and method |
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| Publication Number | Publication Date |
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| CN110476728A CN110476728A (en) | 2019-11-22 |
| CN110476728B true CN110476728B (en) | 2023-12-05 |
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| CN113978780B (en) * | 2021-09-22 | 2023-05-02 | 力源新资源开发(广东)有限公司 | Automatic worm egg sorting machine |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103477863A (en) * | 2012-06-07 | 2014-01-01 | 金勇� | Chinese caterpillar fungus-growing device |
| WO2015003548A1 (en) * | 2013-07-12 | 2015-01-15 | 重庆市中药研究院 | Method for cultivating cordyceps indoors |
| CN105766379A (en) * | 2016-03-22 | 2016-07-20 | 广东东阳光药业有限公司 | System and method for cultivating caterpillar fungus |
| CN211558273U (en) * | 2019-08-12 | 2020-09-25 | 北京同仁堂健康药业（青海）有限公司 | Cordyceps species collection system |
-
2019
- 2019-08-12 CN CN201910738541.7A patent/CN110476728B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103477863A (en) * | 2012-06-07 | 2014-01-01 | 金勇� | Chinese caterpillar fungus-growing device |
| WO2015003548A1 (en) * | 2013-07-12 | 2015-01-15 | 重庆市中药研究院 | Method for cultivating cordyceps indoors |
| CN105766379A (en) * | 2016-03-22 | 2016-07-20 | 广东东阳光药业有限公司 | System and method for cultivating caterpillar fungus |
| CN211558273U (en) * | 2019-08-12 | 2020-09-25 | 北京同仁堂健康药业（青海）有限公司 | Cordyceps species collection system |
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