CN110431953A - A kind of polygonatum cyrtonema seedling stem germination accelerating method - Google Patents

A kind of polygonatum cyrtonema seedling stem germination accelerating method Download PDF

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Publication number
CN110431953A
CN110431953A CN201910816403.6A CN201910816403A CN110431953A CN 110431953 A CN110431953 A CN 110431953A CN 201910816403 A CN201910816403 A CN 201910816403A CN 110431953 A CN110431953 A CN 110431953A
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China
Prior art keywords
seedling stem
stem
seedling
auxin
budding
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Pending
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CN201910816403.6A
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Chinese (zh)
Inventor
王华磊
龙建吕
陈松树
李金玲
黄明进
罗夫来
罗春丽
刘红昌
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Guizhou University
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Guizhou University
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Priority to CN201910816403.6A priority Critical patent/CN110431953A/en
Publication of CN110431953A publication Critical patent/CN110431953A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C1/00Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting

Abstract

The invention discloses a kind of polygonatum cyrtonema seedling stem germination accelerating methods, this method are as follows: select annual polygonatum cyrtonema seedling stem, seedling stem wound is smeared with the vegetation for being mixed with carbendazim, configured 20-60mg/L auxin or 20-60mg/L 6-BA are impregnated into seedling stem, so that soak is flooded seedling stem completely, impregnates 36 hours, pull out, it places the reduction of fractions to a common denominator to go out to air-dry, smears plant ash again.The present invention passes through the growth regulator immersion treatment rhizoma polygonati seedling stem of auxin and 6-BA, shorten the time of seedling stem budding emergence, shortening the time can reach 30 days, improve seedling stem budding and emergence rate, the stem budding of auxin and emergence rate improve 47%, the stem budding of 6-BA and emergence rate improve 34%, it is effective to shorten the seedling stem growth and development period, increase photosynthesis area, freight volume of the increase source to library substance, improve seedling stem yield, conducive to the sustainable development of rhizoma polygonati large-scale planting, test proves that the present invention can effectively improve the budding number of seedling stem, auxin and 6-BA promote the growth of newborn kind of stem eye and root accordingly.

Description

A kind of polygonatum cyrtonema seedling stem germination accelerating method
Technical field
The present invention relates to a kind of polygonatum cyrtonema seedling stem germination accelerating methods, belong to polygonatum cyrtonema seedling stem Stratification technique field.
Background technique
Polygonatum cyrtonema Polygonatum cyrtonema Hua. is Liliaceae Polygonatum Polygonatum Mill. more years Raw herbaceous plant is that 2015 editions " Chinese Pharmacopoeias " record one of 3 rhizoma polygonati Original plants.With boosting qi and nourishing yin, invigorating the spleen, moistening lung, The effect of kidney-nourishing.For deficiency of spleen-QI and stomach-QI, fatigue and asthenia, deficiency of stomach-Yin, dry deficiency of food, deficiency syndrome of the lung cough caused by dryness, overstrain cough hemoptysis, waist and knee acid It is soft, poliosis, Heat Diabetes.
Polygonatum cyrtonema not only can be used as medicinal, but also can be used as that tonic is edible, and rhizoma polygonati plant is alternatively arranged as indoor appreciation plant Object.Domestic market demand amount increases year by year, or even also to export Japan, South Korea, and the market price is higher and in rising trend.In order to Meet market demand, people expand polygonatum cyrtonema artificial growth in recent years, but planting scale is small, and reason is polygonatum cyrtonema Seedling is insufficient, and rhizoma polygonati growth cycle is too long, and input cost is high, and benefit is low.Based on artificial growth is mainly bred with stem tuber, The material used of polygonatum cyrtonema scale plantation at present mainly excavates wild rhizoma polygonati resource to purchase peasant, although propagation of seed stock compared with Sapling multiplication has certain advantage, but the scarcity of wild resource and the growth cycle of seedling stem nursery are still that limitation rhizoma polygonati is extensive One of the reason of plantation, the growth cycle of seedling stem nursery, seedling stem germination are few.
Summary of the invention
The technical problem to be solved by the present invention is a kind of polygonatum cyrtonema seedling stem germination accelerating method is provided, it is above-mentioned existing to solve The problem of technology.
The technical scheme adopted by the invention is as follows: a kind of polygonatum cyrtonema seedling stem germination accelerating method, this method are as follows: select annual more Flower rhizoma polygonati seedling stem, seedling stem wound is smeared with the vegetation for being mixed with carbendazim, by configured 40-60mg/L auxin or 40-60mg/ L 6-BA impregnates seedling stem, and soak is made to flood seedling stem completely, impregnates 36 hours, pulls out, places the reduction of fractions to a common denominator and goes out to air-dry, smears grass again Wood ash.
Beneficial effects of the present invention: compared with prior art, the present invention passes through the leaching of the growth regulator of auxin and 6-BA Processing rhizoma polygonati seedling stem is steeped, the time of seedling stem budding emergence is shortened, the shortening time can reach 30 days, improve seedling stem budding and emergence Rate, the stem budding of auxin and emergence rate improve the stem budding of 47%, 6-BA and emergence rate improves 34%, effectively shorten seedling stem The growth and development period increases photosynthesis area, increases freight volume of the source to library substance, improves seedling stem yield, is conducive to rhizoma polygonati and advises The sustainable development of modelling plantation, test prove that the present invention can effectively improve the budding number of seedling stem, auxin and 6-BA phase The growth of the promotion answered newborn kind of stem eye and root.
Specific embodiment
The present invention is described further below with reference to specific embodiment.
Embodiment 1: a kind of polygonatum cyrtonema seedling stem germination accelerating method, this method are as follows: select annual polygonatum cyrtonema seedling stem, seedling stem Wound is smeared with the vegetation for being mixed with carbendazim, and configured 20mg/L auxin or 20mg/L 6-BA are impregnated seedling stem, make to impregnate Liquid floods seedling stem completely, impregnates 36 hours, pulls out, places the reduction of fractions to a common denominator and goes out to air-dry, smears plant ash again.
Embodiment 2: a kind of polygonatum cyrtonema seedling stem germination accelerating method, this method are as follows: select annual polygonatum cyrtonema seedling stem, seedling stem Wound is smeared with the vegetation for being mixed with carbendazim, and configured 40mg/L auxin or 40mg/L 6-BA are impregnated seedling stem, make to impregnate Liquid floods seedling stem completely, impregnates 36 hours, pulls out, places the reduction of fractions to a common denominator and goes out to air-dry, smears plant ash again.
Embodiment 3: a kind of polygonatum cyrtonema seedling stem germination accelerating method, this method are as follows: select annual polygonatum cyrtonema seedling stem, seedling stem Wound is smeared with the vegetation for being mixed with carbendazim, and configured 60mg/L auxin or 60mg/L 6-BA are impregnated seedling stem, make to impregnate Liquid floods seedling stem completely, impregnates 36 hours, pulls out, places the reduction of fractions to a common denominator and goes out to air-dry, smears plant ash again.
In order to illustrate beneficial effects of the present invention, tested as follows:
(1) it selection of land site preparation: experimental field selects in Guiyang City, Guizhou Province Huaxi District Guizhou University's teaching test base, removes field Topsoil with rotary cultivator or is manually renovated and is flattened by weeds and branch.Divide district's groups and cell, it is district's groups spacing 40cm, small Section is away from being respectively 20cm and 1m with area2.The long side of experimental plot is parallel with change of soil fertility direction, district's groups long side with Change of soil fertility direction is vertical.
(2) preparation of seedling stem material: the rhizoma polygonati seedling stem adopted back is separated by different age, selects growing way consistent annual It does experiment material with the biennial seedling stem without bud head.Seedling stem wound is smeared with the vegetation for being mixed with carbendazim, so as not to it is mouldy.
(3) seedling stem is handled: will get seedling stem material ready by 90 stem tuber separation of each processing.It is soaked with growth regulating agent solution Bubble processing, the concentration of auxin, 6-BA and Gibberellins solution respectively set 3 levels, and each level concentration is respectively as follows: 20mg/L, 40mg/ L, 60mg/L, 3 repetitions of each level repeat 30 seedling stems every time.The growth regulating agent solution of configuration is soaked respectively bubble 1 year Raw and biennial seedling stem makes soak flood seedling stem completely, pulls out after impregnating 36 hours, places the reduction of fractions to a common denominator and goes out to air-dry, smears again Plant ash.
(4) sow: the sowing time is in November, 2018, and using the seeding method of drilling, each cell opens 6 bar ditch, ditch Deep 8~10cm;Every bar ditch organic fertilizer 300g sows 5 stem tubers;Putting for seedling stem is placed for conventional flat pendulum, and bud head upward, is planted In the ranks distance is 25cm, spacing in the rows 20cm to stem.The pine needle heat preservation and moisturizing of 3~5cm of covering after having sowed.
(5) phenological period is observed: observation seedling stem budding situation, later period are primary every 15d observation at the beginning of 3 months next year of sowing.
(6) economical character index determining: after seedling stem budding, each cell randomly selects 10 plants of seedling stems measurement bud head numbers, bud Length, bud diameter, newborn seedling stem the length with item number, the diameter of long, the new seedling stem of main root and newborn seedling stem.
(7) interpretation of result: as shown in Table 1 gibberellin and auxin to the influence degrees of each growth indexes of annual seedling stem compared with Greatly, 6-BA is smaller.Gibberellin has significant inhibiting effect, concentration to the growth of kind of a stem eye length, bud diameter and newborn seedling stem root Higher inhibiting effect enhancing;Auxin has facilitation to the growth of each index of seedling stem, significant to seedling stem budding number effect, right Bud length, bud diameter and newborn seedling stem root item number effect be not significant;6-BA has certain facilitation to each growth indexes of seedling stem, But facilitation is not significant.Gibberellin, auxin and 6-BA also have difference to the growth of biennial seedling stem, are shown in Table 2, gibberellin Inhibit each index growth of seedling stem, the growth of seedling stem is influenced big compared with auxin by gibberellin and 6-BA.Gibberellin sprouts to seedling stem The inhibiting effect of number, bud diameter and newborn seedling stem root item number is significant;6-BA promotes the formation effect of seedling stem sprouting significant, to bud The growth-promoting effect of long, bud diameter and newborn seedling stem root is not significant;The formation of auxin kind stem eye, bud be long, bud diameter and new The growth of non-hibernating eggs stem root item has corresponding facilitation, but its facilitation is not significant.
The influence of growth indexes after 1 gibberellin of table, auxin and 6-BA sprout to annual seedling stem
The influence of growth indexes after 2 gibberellin of table, auxin and 6-BA sprout to biennial seedling stem
The above description is merely a specific embodiment, but scope of protection of the present invention is not limited thereto, any Those familiar with the art in the technical scope disclosed by the present invention, can easily think of the change or the replacement, and should all contain Within protection scope of the present invention, therefore, protection scope of the present invention should be based on the protection scope of the described claims lid.

Claims (1)

1. a kind of polygonatum cyrtonema seedling stem germination accelerating method, it is characterised in that: this method are as follows: select annual polygonatum cyrtonema seedling stem, plant Stem wound is smeared with the vegetation for being mixed with carbendazim, and configured 40-60mg/L auxin or 40-60mg/L 6-BA are impregnated kind Stem makes soak flood seedling stem completely, impregnates 36 hours, pulls out, places the reduction of fractions to a common denominator and goes out to air-dry, smears plant ash again.
CN201910816403.6A 2019-08-30 2019-08-30 A kind of polygonatum cyrtonema seedling stem germination accelerating method Pending CN110431953A (en)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115413545A (en) * 2022-09-05 2022-12-02 钟子龙 Breeding method of Polygonum multiflorum Thunb with one stem and multiple seedlings

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CN105917877A (en) * 2016-04-13 2016-09-07 大理为民中草药种植有限公司 Polygonatum kingianum root stem height segment breeding method
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Application publication date: 20191112