CN110367023A - A method of promoting plant lignifying of sowing grass seeds by duster - Google Patents

A method of promoting plant lignifying of sowing grass seeds by duster Download PDF

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Publication number
CN110367023A
CN110367023A CN201910761956.6A CN201910761956A CN110367023A CN 110367023 A CN110367023 A CN 110367023A CN 201910761956 A CN201910761956 A CN 201910761956A CN 110367023 A CN110367023 A CN 110367023A
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plant
shell
duster
lignifying
grass seeds
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CN110367023B (en
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孙雅楠
王酉宁
刘立勋
陈敏
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Dalian Earth Environment Technology Co Ltd
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Dalian Earth Environment Technology Co Ltd
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C21/00—Methods of fertilising, sowing or planting
    • A01C21/005—Following a specific plan, e.g. pattern
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
    • A01G17/005—Cultivation methods
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
    • A01G24/25—Dry fruit hulls or husks, e.g. chaff or coir
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • A01G24/28—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/10—Animals; Substances produced thereby or obtained therefrom
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05C—NITROGENOUS FERTILISERS
    • C05C3/00—Fertilisers containing other salts of ammonia or ammonia itself, e.g. gas liquor
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F3/00—Fertilisers from human or animal excrements, e.g. manure
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00—Fertilisers characterised by their form
    • C05G5/20—Liquid fertilisers

Abstract

It sows grass seeds by duster the method for plant lignifying the present invention provides a kind of promotion, including preparation is sowed grass seeds by duster hypothallus and seed layer；When seed sprouting, the activation shell class bacterium solution after dilution is uniformly sprayed to emergence area；After seedling grows true leaf and lateral root, compound fertilizer A is applied in soil surface；When nursery stock enters the rapid growth phase, compound fertilizer B is applied in soil surface；After seedling growth speed slows down, brown alga extracting solution is applied to the surface in plant and soil；Before frozen soil, antifreeze covering is carried out to nursery stock.The present invention takes different fertilizing methods, with when concentration in the different phase of plant growth, homemade base fertilizer, bacterium solution, extracting solution are used simultaneously, plant growth each stage is not only met to the different demands of nutrition, it provides simultaneously and has adjusted plant in the need fertilizer proportion of different phase, increase the bacterium solution for promoting plant lignifying, so that plant is accelerated lignifying, enhance cold-resistant resistance, guarantees the smooth overwintering and next year survival rate of plant.

Description

A method of promoting plant lignifying of sowing grass seeds by duster
Technical field
The present invention relates to plant growth and fertilizer field, specifically a kind of promotion is sowed grass seeds by duster the method for plant lignifying.
Background technique
With the fast development of economic society, Chinese capital construction speed is accelerated, because implementing traffic, water conservancy, mine, electricity The construction projects such as power and form a large amount of bare side sloping.These bare side slopings not only affect Landscape Ecological, some are also There are geological disaster hidden danger, the safety and stability of main body afforestation project is influenced, therefore many places start Efforts To Develop side slope and sow grass seeds by duster Greening.Because simple draft greening meeting is so that structure of plant community is highly unstable, promotion uses this plant of vegetation at present The common spraying and seeding afforesting of object, but due to herbaceous plant Overgrowth, xylophyta will soon occur nutrition, sunlight, moisture and its Phenomena such as his inadequate resource, when herbaceous plant the nutrient consumption in soil to a certain extent, side slope will will appear large area Decline, or even come back to the state of bare area.On the other hand, due to the weather in project under construction area, edaphic condition, grade of side slope etc. The uncertainty of factor, germination, growth and the lignifying process of the xylophyta in matrix that influences to sow grass seeds by duster, so as to cause woody It is serious to affect nursery stock cold-resistant antifreeze ability before plant nursery stock arrives in winter because without sufficient time lignifying Difference, this nursery stock is often because occurred very low survival rate in the second year beginning of spring by freeze injury.It is supported for this purpose, to formulate the rational application of fertilizer Management measure is protected, to guarantee the growth and lignifying demand of xylophyta, to promote phytobiocoenose success to convert to objective community.
Summary of the invention
It sows grass seeds by duster the method for plant lignifying the purpose of the present invention is to provide a kind of promotion, to solve in above-mentioned background technique There are the problem of.
The technical scheme of the present invention is realized as follows:
A method of promoting plant lignifying of sowing grass seeds by duster, comprising the following steps:
(1) by 5%~10% base fertilizer, 5%~8% laminarin, 2%~4% plant fiber, 10%~15% husk, 10%~20% rotted leaf Soil, 15%~20% pine soil be mixed, 2%~5% microbial flocculant, 15%~18% domestic sludge, 8%~10% haydite, 5%~13% grass Wood ash is uniformly mixed, and preparation is sowed grass seeds by duster hypothallus, is sowed grass seeds by duster seed layer in substrate layer surface, seed layer timely sprinkling maintenance after sowing grass seeds by duster, Daily morning and evening twice；
(2) when seed sprouting, by activation shell class bacterium solution according to 1:(150~250) it is diluted in water, by the activation shell after dilution Class bacterium solution is uniformly sprayed to emergence area；
(3) 7~14 days after true leaf occurs in seedling aerial part, underground part bears lateral root, compound fertilizer A is applied in soil table Face, water seasoning after 1~2 day；
(4) when nursery stock enters the rapid growth phase, compound fertilizer B is applied in soil surface, water seasoning after 1~2 day, excessively 7~ After 14 days foliar fertilizer can be sprayed on plant surface according to plant growth situation；
(5) after seedling growth speed obviously slows down, stop frequently watering, brown alga extracting solution is applied in plant and soil Surface, water seasoning after 3~5 days；
(6) before frozen soil, antifreeze covering is carried out using straw, wheat straw to nursery stock, guarantees that soil continues to certain temperature, Make nursery stock being capable of abundant lignifying before entering the winter.
Preferably, step (1) base fertilizer is made of the raw material of following mass fraction: 15%~25% discarded shell powder, 10%~ 15% brown alga powder, 15%~20% guano, 10%~15% green manure, 5%~15% cow dung, 25%~30% agriculture and forestry organic waste material.
Preferably, the preparation method of step (2) the activation shell class bacterium solution, comprising the following steps:
A. the shell of the discarded marine organisms such as Carapax Eriocheir sinensis, shrimp shell, shellfish shell, conch shell is collected, is sent into vaporium and carries out high temperature height Disinfection and sterilization is pressed, it is 350 DEG C~400 DEG C that control, which is passed through steam temperature, and control pressure value is 0.25 Mpa~0.35Mpa；
B. the shell substance after step A autoclave sterilization is sent into room temperature in washing roller machine and cleans 30min~40min, and Collect liquid after cleaning；
C. shell matter after step B cleaning is sent into cylinder crusher to be crushed, guarantees that broken rear substance diameter is 1cm~2cm;
D. dryer will be sent into after the broken shell substance washing of step C and carries out water removal drying, be re-fed into flour mill and carry out powder Mill, obtains filtered fluid and discarded shell powder, and the discarded shell powder is used as the raw material of base fertilizer preparation；
E. liquid and step D filtered fluid after step B cleaning are mixed evenly, be added the filtrate of discarded fruits and vegetables, polyvinyl alcohol, Glucose, vegetation buck, neopelex obtain mixed liquor；
F. step E mixed liquor is taken out quantitatively addition fermentation medium, shaking table culture for 24 hours~36h, adjust mixed liquor mushroom concentration It is 99Cfu/ml~119cfu/ml；
G. the mixed liquor after step F culture is sent into stirring fermenter and is fermented, keeping temperature within the barrel is 55 DEG C~65 DEG C, Fermentation time is for 24 hours~48h, and mixing speed is 180r/min~220r/min, and ventilatory capacity is 2.5L/min~3.5L/min；
H. the mixed liquor after step G fermentation is subjected to ultraviolet light and dithyl sulfate complex mutation, obtains activation shell class bacterium solution.
Preferably, step (3) compound fertilizer's A nitrogen phosphoris and potassium fertilizer ratio is 1:2:2, and fertilising concentration is 1%~3%, and the amount of applying is 10 g/m2~15g/m2, applying frequency is 10~15 days/time.
Preferably, it is 1:1:1 that the step, which answers (4) compound fertilizer B nitrogen phosphoris and potassium fertilizer ratio, and fertilising concentration is 1.5%~4%, is applied Amount is 15 g/m2~20g/m2, applying frequency is 14~21 days/time.
Preferably, step (4) foliar fertilizer can determine to apply dosage and type according to the growing state of plant, to plant Object face of blade and reverse side spray that even, spray is thin, available foliar fertilizer are as follows: urea that concentration is 0.5%~2%, concentration are Manganese chloride that borax that 0.2%~1.0% ferrous sulfate, concentration are 0.3%~0.5%, concentration are 0.05%~0.10%, Zinc sulfate that copper sulphate that concentration is 0.02%~0.04%, concentration are 0.1%~0.5%, concentration are 0.02%~0.05% Ammonium molybdate etc..
Preferably, the preparation method of step (5) the brown alga extracting solution, comprising the following steps:
A. it is sent into pulper after mixing thallus laminariae, sargassum according to 1:1 to blend, obtains mixture；
B. step a mixture is sent into grinder and carries out fine gtinding, obtain paste；
C. step b paste is filtered, obtains sieving paste and sieves lower filtrate；
D. paste on step c sieve is dried and is exposed to the sun, obtain brown alga powder, the brown alga powder is used as the raw material of base fertilizer preparation；
E. step c is sieved into lower filtrate and is sent into supercentrifuge progress centrifugation, obtain centrifugate；
F. step e centrifugate is reinforced into water-bath concentration, obtains concentrate；
G. step f concentrate is sent into electro-heating standing-temperature cultivator, heating culture obtains culture；
H. it is down to after room temperature after step g culture being taken out and alcohol is added, then low temperature clarification, obtain clarified solution；
I. it will be added after ether heating water bath to be concentrated in step h clarified solution and extract, it is stored refrigerated, obtain brown alga extracting solution.
Preferably, the revolving speed of the step e supercentrifuge is 1900 turns per minute~2100 turns, centrifugation time 10 Min~20min.
Preferably, the temperature of the step f electro-heating standing-temperature cultivator is set as 80 DEG C~100 DEG C, keeps the temperature 20 The min of min~40.
Preferably, the temperature of the step h low temperature clarification is set as 0 DEG C~6 DEG C, keeps temperature 18h~24 h.
The invention has the benefit that
1, the present invention takes different fertilizing methods, with when concentration in the different phase of plant growth, while using self-control Base fertilizer, bacterium solution, extracting solution, not only meet plant growth each stage to the different demands of nutrition, while providing and having adjusted plant Object is matched in the need fertilizer of different phase, increases the bacterium solution for promoting plant lignifying, and plant is made to accelerate lignifying, and enhancing cold-resistant is anti- Inverse property guarantees the smooth overwintering and next year survival rate of plant.
2, the present invention prepares base fertilizer raw material using the shell for discarding marine organisms in life, provides abundance for soil of sowing grass seeds by duster The elements such as calcium, carbon, oxygen, hydrogen, strontium, magnesium also provide the organic matters such as glycoprotein and polysaccharide, help to improve plant and bear in frost It by the ability of desiccation stress, reduces cell and freezes dehydration, while utilizing the cleaning solution in treatment process and filtered fluid preparation activation Shell class bacterium solution forms profitable strain after spray on plants in the soil, and activating soil greatly enhances the resistance of plant, in addition Discarded marine organisms are combined into microbial fermentation engineering, ocean shell class biological synthesis is developed and used, reduces waste to ring The pollution in border, turns waste into wealth, and economizes on resources.
3, the present invention extracts brown alga powder and brown alga extracting solution using brown alga, is respectively used to base fertilizer and plant before sowing grass seeds by duster in base The preparation for the Liquid Fertilizer that object Later growth sprays, the brown alga powder prepared by this method, which has, is largely conducive to plant growth hair The natural bioactivity substance educated and seaweed absorb from ocean and are enriched in intracorporal mineral nutrient element, while preparing Containing the plant growth regulating substance of high concentration in brown alga extracting solution, plant can be being effectively promoted rapidly in the plant growth later period The rapid lignifying of object root growth, and contain a large amount of disease-resistant factors and special composition, significantly increase the degeneration-resistant disease resistance of plant.
Specific embodiment
Technical solution of the present invention is clearly and completely described below in conjunction with embodiment, it is clear that implement describedly Example is only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, this field is common Technical staff's every other embodiment obtained without making creative work belongs to the model that the present invention protects It encloses.
Embodiment 1
A method of promoting plant lignifying of sowing grass seeds by duster, comprising the following steps:
(1) shell of the discarded marine organisms such as Carapax Eriocheir sinensis, shrimp shell, shellfish shell, conch shell is collected, is sent into vaporium and carries out high temperature height Disinfection and sterilization is pressed, it is 350 DEG C that control, which is passed through steam temperature, and control pressure value is 0.25 Mpa, is re-fed into washing roller machine Room temperature cleans 40min, and collects liquid after cleaning；
(2) shell matter after step B cleaning cylinder crusher is sent into be crushed, guarantee it is broken after substance diameter be 1cm~ 2cm carries out water removal drying for dryer is sent into after the washing of broken shell substance, is re-fed into flour mill and carries out grinding, obtain Filtered fluid and discarded shell powder；
(3) liquid after step (1) cleaning and step (2) filtered fluid are mixed evenly, the filtrate for discarding fruits and vegetables, poly- second is added Enol, glucose, vegetation buck, neopelex obtain mixed liquor, are taken out quantitatively mixed liquor and fermentation medium is added, For 24 hours, adjustment mixed liquor mushroom concentration is 9 to shaking table culture9Cfu/ml~119Cfu/ml is re-fed into stirring fermenter and is sent out Ferment, keeping temperature within the barrel is 55 DEG C, fermentation time 48h, mixing speed 180r/min, ventilatory capacity 2.5L/min, finally will Mixed liquor after fermentation carries out ultraviolet light and dithyl sulfate complex mutation, obtains activation shell class bacterium solution；
(4) after thallus laminariae, sargassum being mixed according to 1:1 be sent into pulper blend, by obtained mixture be sent into grinder into Row fine gtinding will obtain paste and be filtered, and obtains sieving paste and sieves lower filtrate, will sieve upper paste drying and sudden and violent It shines, obtains brown alga powder；
(5) lower filtrate will be sieved and be sent into supercentrifuge progress centrifugation, control revolving speed is 1900 turns per minute, and centrifugation time is 20min, then centrifugate will be obtained and reinforce concentration in water-bath, then concentrate will be obtained and be sent into electro-heating standing-temperature cultivator heating training It supports, temperature is set as 80 DEG C, 40 min of the temperature is kept, then will obtain that alcohol is added after being down to room temperature after culture takes out, then In 0 DEG C of 24 h of low temperature clarification, will obtain that extraction is concentrated after ether heating water bath is added in clarified solution, it is stored refrigerated, obtain brown alga Extracting solution；
(6) by the discarded shell powder of step (2) 15%, (4) 10% brown alga powder of step, 15% guano, 15% green manure, 15% cow dung, 30% agriculture Woods waste is uniformly mixed and prepares base fertilizer；
(7) mix (6) 10% base fertilizer of step, 8% laminarin, 2% plant fiber, 15% husk, 20% leaf mould, 15% pine soil, 2% microbial flocculant, 15% domestic sludge, 8% haydite, 5% plant ash are uniformly mixed, and hypothallus of sowing grass seeds by duster are prepared, in hypothallus table Face is sowed grass seeds by duster seed layer, seed layer timely sprinkling maintenance after sowing grass seeds by duster, daily morning and evening twice；
(8) when seed sprouting, step (3) activation shell class bacterium solution is diluted in water according to 1:150, by the activation shell after dilution Class bacterium solution is uniformly sprayed to emergence area；
(9) 7 days after true leaf occurs in seedling aerial part, underground part bears lateral root, compound fertilizer A is applied in soil surface, 1~ Water seasoning after 2 days, compound fertilizer's A nitrogen phosphoris and potassium fertilizer ratio are 1:2:2, and fertilising concentration is 1%, and the amount of applying is 15g/m2, applying frequency is 10 days/time；
(10) when nursery stock enters the rapid growth phase, compound fertilizer B is applied in soil surface, water seasoning after 1 day, after spending 7 days Foliar fertilizer can be sprayed on plant surface, compound fertilizer's B nitrogen phosphoris and potassium fertilizer ratio is 1:1:1, and fertilising concentration is according to plant growth situation 1.5%, the amount of applying is 20g/m2, applying frequency is 14 days/time；
(11) after seedling growth speed obviously slows down, stop frequently watering, step (5) brown alga extracting solution is applied in plant With the surface of soil, water seasoning after 3 days；
(12) before frozen soil, antifreeze covering is carried out using straw, wheat straw to nursery stock, guarantees that soil continues to certain temperature Degree, makes nursery stock being capable of abundant lignifying before entering the winter.
Embodiment 2
A method of promoting plant lignifying of sowing grass seeds by duster, comprising the following steps:
(1) shell of the discarded marine organisms such as Carapax Eriocheir sinensis, shrimp shell, shellfish shell, conch shell is collected, is sent into vaporium and carries out high temperature height Disinfection and sterilization is pressed, it is 370 DEG C that control, which is passed through steam temperature, and control pressure value is 0.30Mpa, is re-fed into washing roller machine often Temperature cleaning 35min, and collect liquid after cleaning；
(2) shell matter after step B cleaning cylinder crusher is sent into be crushed, guarantee it is broken after substance diameter be 1cm~ 2cm carries out water removal drying for dryer is sent into after the washing of broken shell substance, is re-fed into flour mill and carries out grinding, obtain Filtered fluid and discarded shell powder；
(3) liquid after step (1) cleaning and step (2) filtered fluid are mixed evenly, the filtrate for discarding fruits and vegetables, poly- second is added Enol, glucose, vegetation buck, neopelex obtain mixed liquor, are taken out quantitatively mixed liquor and fermentation medium is added, Shaking table culture 30h, adjustment mixed liquor mushroom concentration are 99Cfu/ml~119Cfu/ml is re-fed into stirring fermenter and is sent out Ferment, keeping temperature within the barrel is 60 DEG C, fermentation time 36h, mixing speed 200r/min, ventilatory capacity 3L/min, finally will hair Mixed liquor after ferment carries out ultraviolet light and dithyl sulfate complex mutation, obtains activation shell class bacterium solution；
(4) after thallus laminariae, sargassum being mixed according to 1:1 be sent into pulper blend, by obtained mixture be sent into grinder into Row fine gtinding will obtain paste and be filtered, and obtains sieving paste and sieves lower filtrate, will sieve upper paste drying and sudden and violent It shines, obtains brown alga powder；
(5) lower filtrate will be sieved and be sent into supercentrifuge progress centrifugation, control revolving speed is 2000 turns per minute, and centrifugation time is 15min, then centrifugate will be obtained and reinforce concentration in water-bath, then concentrate will be obtained and be sent into electro-heating standing-temperature cultivator heating training It supports, temperature is set as 90 DEG C, temperature 30min is kept, then will obtain being down to after room temperature after culture takes out and alcohol is added, then 3 DEG C of 21 h of low temperature clarification will obtain that extraction is concentrated after ether heating water bath is added in clarified solution, stored refrigerated, obtains brown alga and mentions Take liquid；
(6) by the discarded shell powder of step (2) 20%, (4) 13% brown alga powder of step, 17% guano, 12% green manure, 10% cow dung, 28% agriculture Woods waste is uniformly mixed and prepares base fertilizer；
(7) by (6) 8% base fertilizer of step, 7% laminarin, 3% plant fiber, 12% husk, 15% leaf mould, 18% pine soil it is mixed, 4% Microbial flocculant, 16% domestic sludge, 9% haydite, 7% plant ash are uniformly mixed, and hypothallus of sowing grass seeds by duster are prepared, in substrate layer surface It sows grass seeds by duster seed layer, seed layer timely sprinkling maintenance after sowing grass seeds by duster, daily morning and evening twice；
(8) when seed sprouting, step (3) activation shell class bacterium solution is diluted in water according to 1:200, by the activation shell after dilution Class bacterium solution is uniformly sprayed to emergence area；
(9) 10 days after true leaf occurs in seedling aerial part, underground part bears lateral root, compound fertilizer A is applied in soil surface, 1 Water seasoning after it, compound fertilizer's A nitrogen phosphoris and potassium fertilizer ratio are 1:2:2, and fertilising concentration is 2%, and the amount of applying is 12g/m2, applying frequency is 13 days/time；
(10) when nursery stock enters the rapid growth phase, compound fertilizer B is applied in soil surface, water seasoning after 2 days, after spending 10 days Foliar fertilizer can be sprayed on plant surface, compound fertilizer's B nitrogen phosphoris and potassium fertilizer ratio is 1:1:1, and fertilising concentration is according to plant growth situation 2.7%, the amount of applying is 17g/m2, applying frequency is 18 days/time；
(11) after seedling growth speed obviously slows down, stop frequently watering, step (5) brown alga extracting solution is applied in plant With the surface of soil, water seasoning after 4 days；
(12) before frozen soil, antifreeze covering is carried out using straw, wheat straw to nursery stock, guarantees that soil continues to certain temperature Degree, makes nursery stock being capable of abundant lignifying before entering the winter.
Embodiment 3
A method of promoting plant lignifying of sowing grass seeds by duster, comprising the following steps:
(1) shell of the discarded marine organisms such as Carapax Eriocheir sinensis, shrimp shell, shellfish shell, conch shell is collected, is sent into vaporium and carries out high temperature height Disinfection and sterilization is pressed, it is 400 DEG C that control, which is passed through steam temperature, and control pressure value is 0.35Mpa, is re-fed into washing roller machine often Temperature cleaning 30min, and collect liquid after cleaning；
(2) shell matter after step B cleaning cylinder crusher is sent into be crushed, guarantee it is broken after substance diameter be 1cm~ 2cm carries out water removal drying for dryer is sent into after the washing of broken shell substance, is re-fed into flour mill and carries out grinding, obtain Filtered fluid and discarded shell powder；
(3) liquid after step (1) cleaning and step (2) filtered fluid are mixed evenly, the filtrate for discarding fruits and vegetables, poly- second is added Enol, glucose, vegetation buck, neopelex obtain mixed liquor, are taken out quantitatively mixed liquor and fermentation medium is added, Shaking table culture 36h, adjustment mixed liquor mushroom concentration are 99Cfu/ml~119Cfu/ml is re-fed into stirring fermenter and is sent out Ferment, keeping temperature within the barrel is 65 DEG C, and fermentation time is mixing speed 220r/min, ventilatory capacity 3.5L/min for 24 hours, finally will Mixed liquor after fermentation carries out ultraviolet light and dithyl sulfate complex mutation, obtains activation shell class bacterium solution；
(4) after thallus laminariae, sargassum being mixed according to 1:1 be sent into pulper blend, by obtained mixture be sent into grinder into Row fine gtinding will obtain paste and be filtered, and obtains sieving paste and sieves lower filtrate, will sieve upper paste drying and sudden and violent It shines, obtains brown alga powder；
(5) lower filtrate will be sieved and be sent into supercentrifuge progress centrifugation, control revolving speed is 2100 turns per minute, and centrifugation time is 10 min, then centrifugate will be obtained and reinforce concentration in water-bath, then concentrate will be obtained and be sent into electro-heating standing-temperature cultivator heating training It supports, temperature is set as 100 DEG C, 20 min of the temperature is kept, then will obtain that alcohol is added after being down to room temperature after culture takes out, then In 6 DEG C of low temperature clarification 18h, will obtain that extraction is concentrated after ether heating water bath is added in clarified solution, it is stored refrigerated, it obtains brown alga and mentions Take liquid；
(6) by the discarded shell powder of step (2) 25%, (4) 10% brown alga powder of step, 20% guano, 10% green manure, 5% cow dung, 30% agricultural Waste is uniformly mixed and prepares base fertilizer；
(7) by (6) 5% base fertilizer of step, 5% laminarin, 4% plant fiber, 10% husk, 10% leaf mould, 20% pine soil it is mixed, 5% Microbial flocculant, 18% domestic sludge, 10% haydite, 13% plant ash are uniformly mixed, and hypothallus of sowing grass seeds by duster are prepared, in hypothallus table Face is sowed grass seeds by duster seed layer, seed layer timely sprinkling maintenance after sowing grass seeds by duster, daily morning and evening twice；
(8) when seed sprouting, step (3) activation shell class bacterium solution is diluted in water according to 1:250, by the activation after dilution Shell class bacterium solution is uniformly sprayed to emergence area；
(9) 14 days after true leaf occurs in seedling aerial part, underground part bears lateral root, compound fertilizer A is applied in soil surface, 2 Water seasoning after it, compound fertilizer's A nitrogen phosphoris and potassium fertilizer ratio are 1:2:2, and fertilising concentration is 3%, and the amount of applying is 10g/m2, applying frequency is 15 days/time；
(10) when nursery stock enters the rapid growth phase, compound fertilizer B is applied in soil surface, water seasoning after 2 days, spends 14 days After can spray foliar fertilizer on plant surface, compound fertilizer's B nitrogen phosphoris and potassium fertilizer ratio is 1:1:1, concentration of applying fertilizer according to plant growth situation It is 4%, the amount of applying is 15g/m2, applying frequency is 21 days/time；
(11) after seedling growth speed obviously slows down, stop frequently watering, step (5) brown alga extracting solution is applied in plant With the surface of soil, water seasoning after 5 days；
(12) before frozen soil, antifreeze covering is carried out using straw, wheat straw to nursery stock, guarantees that soil continues to certain temperature Degree, makes nursery stock being capable of abundant lignifying before entering the winter.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention Within mind and principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.

Claims (10)

  1. A kind of method of plant lignifying 1. promotion is sowed grass seeds by duster, comprising the following steps:
    (1) by 5%~10% base fertilizer, 5%~8% laminarin, 2%~4% plant fiber, 10%~15% husk, 10%~20% rotted leaf Soil, 15%~20% pine soil be mixed, 2%~5% microbial flocculant, 15%~18% domestic sludge, 8%~10% haydite, 5%~13% grass Wood ash is uniformly mixed, and preparation is sowed grass seeds by duster hypothallus, is sowed grass seeds by duster seed layer in substrate layer surface, seed layer timely sprinkling maintenance after sowing grass seeds by duster, Daily morning and evening twice；
    (2) when seed sprouting, by activation shell class bacterium solution according to 1:(150~250) it is diluted in water, by the activation shell after dilution Class bacterium solution is uniformly sprayed to emergence area；
    (3) 7~14 days after true leaf occurs in seedling aerial part, underground part bears lateral root, compound fertilizer A is applied in soil table Face, water seasoning after 1~2 day；
    (4) when nursery stock enters the rapid growth phase, compound fertilizer B is applied in soil surface, water seasoning after 1~2 day, excessively 7~ After 14 days foliar fertilizer can be sprayed on plant surface according to plant growth situation；
    (5) after seedling growth speed obviously slows down, stop frequently watering, brown alga extracting solution is applied in plant and soil Surface, water seasoning after 3~5 days；
    (6) before frozen soil, antifreeze covering is carried out using straw, wheat straw to nursery stock, guarantees that soil continues to certain temperature, Make nursery stock being capable of abundant lignifying before entering the winter.
  2. The method of plant lignifying 2. a kind of promotion according to claim 1 is sowed grass seeds by duster, which is characterized in that the step (1) Base fertilizer is made of the raw material of following mass fraction: 15%~25% discarded shell powder, 10%~15% brown alga powder, 15%~20% guano, 10%~15% green manure, 5%~15% cow dung, 25%~30% agriculture and forestry organic waste material.
  3. The method of plant lignifying 3. a kind of promotion according to claim 1 is sowed grass seeds by duster, which is characterized in that the step (2) Activate the preparation method of shell class bacterium solution, comprising the following steps:
    A. the shell of the discarded marine organisms such as Carapax Eriocheir sinensis, shrimp shell, shellfish shell, conch shell is collected, is sent into vaporium and carries out high temperature height Disinfection and sterilization is pressed, it is 350 DEG C~400 DEG C that control, which is passed through steam temperature, and control pressure value is 0.25 Mpa~0.35Mpa；
    B. the shell substance after step A autoclave sterilization is sent into room temperature in washing roller machine and cleans 30min~40min, and Collect liquid after cleaning；
    C. shell matter after step B cleaning is sent into cylinder crusher to be crushed, guarantees that broken rear substance diameter is 1cm~2cm;
    D. dryer will be sent into after the broken shell substance washing of step C and carries out water removal drying, be re-fed into flour mill and carry out powder Mill obtains filtered fluid and discarded shell powder, the raw material that the discarded shell is prepared as the base fertilizer of claim 2；
    E. liquid and step D filtered fluid after step B cleaning are mixed evenly, be added the filtrate of discarded fruits and vegetables, polyvinyl alcohol, Glucose, vegetation buck, neopelex obtain mixed liquor；
    F. step E mixed liquor is taken out quantitatively addition fermentation medium, shaking table culture for 24 hours~36h, adjust mixed liquor mushroom concentration For the cfu/ml of 99cfu/ml~119；
    G. the mixed liquor after step F culture is sent into stirring fermenter and is fermented, keeping temperature within the barrel is 55 DEG C~65 DEG C, Fermentation time is for 24 hours~48h, and mixing speed is 180r/min~220r/min, and ventilatory capacity is 2.5L/min~3.5L/min；
    H. the mixed liquor after step G fermentation is subjected to ultraviolet light and dithyl sulfate complex mutation, obtains activation shell class bacterium solution.
  4. The method of plant lignifying 4. a kind of promotion according to claim 1 is sowed grass seeds by duster, which is characterized in that the step (3) Compound fertilizer's A nitrogen phosphoris and potassium fertilizer ratio is 1:2:2, and fertilising concentration is 1%~3%, and the amount of applying is 10~15g/m2, and applying frequency is 10~15 Day/time.
  5. The method of plant lignifying 5. a kind of promotion according to claim 1 is sowed grass seeds by duster, which is characterized in that the step is multiple (4) compound fertilizer B nitrogen phosphoris and potassium fertilizer ratio is 1:1:1, and fertilising concentration is 1.5%~4%, and the amount of applying is 15~20g/m2, and applying frequency is 14~21 days/time.
  6. The method of plant lignifying 6. a kind of promotion according to claim 1 is sowed grass seeds by duster, which is characterized in that the step (4) Foliar fertilizer can determine to apply dosage and type according to the growing state of plant, to plant leaf blade obverse and reverse spray it is even, Spray is thin, available foliar fertilizer are as follows: ferrous sulfate that urea that concentration is 0.5%~2%, concentration are 0.2%~1.0%, concentration For 0.3%~0.5% borax, concentration be 0.05%~0.10% manganese chloride, concentration be 0.02%~0.04% sulfuric acid The ammonium molybdate etc. that zinc sulfate that copper, concentration are 0.1%~0.5%, concentration are 0.02%~0.05%.
  7. The method of plant lignifying 7. a kind of promotion according to claim 1 is sowed grass seeds by duster, which is characterized in that the step (5) The preparation method of brown alga extracting solution, comprising the following steps:
    A. it is sent into pulper after mixing thallus laminariae, sargassum according to 1:1 to blend, obtains mixture；
    B. step a mixture is sent into grinder and carries out fine gtinding, obtain paste；
    C. step b paste is filtered, obtains sieving paste and sieves lower filtrate；
    D. paste on step c sieve is dried and is exposed to the sun, obtain brown alga powder, base fertilizer system of the brown alga powder as claim 2 Standby raw material；
    E. step c is sieved into lower filtrate and is sent into supercentrifuge progress centrifugation, obtain centrifugate；
    F. step e centrifugate is reinforced into water-bath concentration, obtains concentrate；
    G. step f concentrate is sent into electro-heating standing-temperature cultivator, heating culture obtains culture；
    H. it is down to after room temperature after step g culture being taken out and alcohol is added, then low temperature clarification, obtain clarified solution；
    I. it will be added after ether heating water bath to be concentrated in step h clarified solution and extract, it is stored refrigerated, obtain brown alga extracting solution.
  8. 8. the preparation method of brown alga extracting solution according to claim 7, which is characterized in that the step e supercentrifuge Revolving speed be 1900 turns per minute~2100 turns, centrifugation time be 10 min~20min.
  9. 9. the preparation method of brown alga extracting solution according to claim 7, which is characterized in that the step f electric heating constant temperature training The temperature for supporting case is set as 80 DEG C~100 DEG C, keeps the temperature 20 min~40 min.
  10. 10. the preparation method of brown alga extracting solution according to claim 7, which is characterized in that the step h low temperature clarification Temperature is set as 0 DEG C~6 DEG C, keeps temperature 18h~24 h.
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