CN110326503A - A kind of identification method of direct-sowing dry rice mesocotyl elongation ability - Google Patents
A kind of identification method of direct-sowing dry rice mesocotyl elongation ability Download PDFInfo
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- CN110326503A CN110326503A CN201910719624.1A CN201910719624A CN110326503A CN 110326503 A CN110326503 A CN 110326503A CN 201910719624 A CN201910719624 A CN 201910719624A CN 110326503 A CN110326503 A CN 110326503A
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- mesocotyl
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- nutrition soil
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- rice
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- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 42
- 235000009566 rice Nutrition 0.000 title claims abstract description 42
- 238000000034 method Methods 0.000 title claims abstract description 22
- 238000009331 sowing Methods 0.000 title claims abstract description 21
- 240000007594 Oryza sativa Species 0.000 title 1
- 241000209094 Oryza Species 0.000 claims abstract description 41
- 235000016709 nutrition Nutrition 0.000 claims abstract description 34
- 230000035764 nutrition Effects 0.000 claims abstract description 34
- 238000005070 sampling Methods 0.000 claims abstract description 3
- 238000012163 sequencing technique Methods 0.000 claims abstract description 3
- 239000002689 soil Substances 0.000 claims description 32
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 17
- 239000000463 material Substances 0.000 claims description 7
- 239000007921 spray Substances 0.000 claims description 6
- 239000008399 tap water Substances 0.000 claims description 3
- 235000020679 tap water Nutrition 0.000 claims description 3
- 238000005303 weighing Methods 0.000 claims description 2
- 238000012136 culture method Methods 0.000 abstract description 10
- 238000012360 testing method Methods 0.000 abstract description 10
- 238000011156 evaluation Methods 0.000 abstract description 3
- 238000012797 qualification Methods 0.000 abstract description 2
- 238000012549 training Methods 0.000 description 8
- 239000004576 sand Substances 0.000 description 6
- 238000004458 analytical method Methods 0.000 description 3
- 230000003020 moisturizing effect Effects 0.000 description 3
- 230000007226 seed germination Effects 0.000 description 3
- 230000007812 deficiency Effects 0.000 description 2
- 230000029553 photosynthesis Effects 0.000 description 2
- 238000010672 photosynthesis Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 235000019994 cava Nutrition 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000009313 farming Methods 0.000 description 1
- 239000011888 foil Substances 0.000 description 1
- 238000012252 genetic analysis Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000000873 masking effect Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000003147 molecular marker Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000012422 test repetition Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/20—Cereals
- A01G22/22—Rice
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Pretreatment Of Seeds And Plants (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a kind of identification methods of direct-sowing dry rice mesocotyl elongation ability, it uses (35 DEG C of high temperature, RH=50%-60%) mesocotyl of the nutrition earth culture method identification rice under low humidity conditions extends ability, observation emergence daily, the sequencing for recording different cultivars emergence, the 3rd day sampling and measuring mesocotyl length after first kind is emerged；Rice shoot is taken out from hole tray in order, is rinsed well, is successively lain in on graduated plate, and take pictures；After the completion of all kinds are successively taken pictures, with Image J software, the mesocotyl length of each kind is measured according to the scale setting ratio ruler on plate.Application in the elongation ability identification of direct-sowing dry rice mesocotyl through the invention, can significantly shorten the test period, improve mesocotyl phenotypic evaluation efficiency, reduce test error caused by human factor.The repeatability and stability of direct-sowing dry rice mesocotyl elongation ability qualification result are substantially increased simultaneously.
Description
Technical field
The present invention relates to field of biotechnology, and in particular to a kind of identification side of direct-sowing dry rice mesocotyl elongation ability
Method.,
Background technique
Direct-sowing dry rice has the advantages that water-saving, saving of labor, to be suitable for mechanized farming etc. a series of but raw in direct-sowing dry rice
In production, due to working depth deficiency, causes late growth stage of rice lodging phenomenon to happen occasionally, to solve this problem, producing
On generally require increase depth of planting.And seed deeply and be conducive to prick under rice root, root system is improved to the absorption energy of moisture and nutrient
Power, while improving the lodging tolerance of rice, but seed deeplying Rice under Condition emergence difficult, reduces rice seedling rate, and by
This leads to rice field Basic Seedling deficiency and weeds control problem.And under the conditions of seed deeplying, seed sprouts that the duration is long, and during which seedling is not
It can be carried out photosynthesis, easily cause growth of seedling weak, spire yellow.In a dark environment after Seed Germination of Rice, mesocotyl passes through
Newborn plumule can be ejected soil by elongation, carried out photosynthesis, improved rice seedling survival rate.In general, long mesocotyl
Kind top soil ability is strong, emergence rate fast speed.Therefore, the physiological regulating control mechanism of mesocotyl elongation is specified, embryo in regulation is excavated
The gene of elongate axis develops corresponding molecular labeling, screens and formulate long mesocotyl germplasm for direct-sowing dry rice molecular
Marker-assisted breeding (MAS) is of great significance.However the control mesocotyl elongation up to the present navigated in different researchs
QTL it is existing very much, but since mesocotyl extends significant with environmental condition interaction, cause experimental result repeatable poor.
Summary of the invention
To solve the above problems, the present invention provides a kind of identification method of direct-sowing dry rice mesocotyl elongation ability, it is real
The stability and repeatability for having showed mesocotyl phenotypic evaluation, to extend the genetic research of ability for direct-sowing dry rice mesocotyl
Stable phenotype is provided.
To achieve the above object, the technical scheme adopted by the invention is as follows:
A kind of identification method of direct-sowing dry rice mesocotyl elongation ability, includes the following steps:
S1, Nutrition Soil was smashed to 5 meshes, stirred evenly spare；
S2, it is packed into the Nutrition Soil that 500g is smashed in pallet, paves, is compacted, hole tray is placed in pallet, in the every of hole tray
The a certain amount of Nutrition Soil smashed and compacting are inserted in a cave, make the difference in height 6cm of native face and pocket；
S3, the full uniform and intact glume rice paddy seed of selection, are sowed, one, every cave kind, Mei Gepin in sequence
Kind broadcasts 15, and 15 seeds are evenly distributed on cave bottom；
The Nutrition Soil smashed uniformly is covered after the completion of S4, all material sowing, until native face is concordant with hole tray mouth, weighs support
The quality summation of disk, hole tray and Nutrition Soil therein and seed, is denoted as W1；
S5, the Nutrition Soil in hole tray is irrigated with the mode of spray, being subject in lower opening has water leakage, then by hole tray from support
It takes out, is poured the Nutrition Soil in pallet to saturation with tap water, and hole tray is put back in pallet, by the overall control of watering in disk
In 2000g, hereafter, the Nutrition Soil height in hole tray is mended to concordant with pocket with the Nutrition Soil smashed, weighing gross weight is W2；
S6, above-mentioned pallet and hole tray are placed in together room temperature be 35 DEG C, relative humidity be 50%-60% dark training
Interior is supported, every 24 hours, spray was primary, controlled the water of spray, and the gross weight after making water drenching is maintained at W2 level；
S7, emergence is observed daily, the sequencing of record different cultivars emergence, the after first kind is emerged
3 days sampling and measuring mesocotyl lengths；Rice shoot is taken out from hole tray in order, is rinsed well, growing way in every part of material is uniform
Seedling successively lie in on graduated plate, and take pictures, should be noted when taking pictures camera position in the surface of plate, it is high
Degree is subject to entire plate is put into view-finder, and with tripod fixed camera position；After the completion of all kinds are successively taken pictures,
With ImageJ software, the mesocotyl length of each kind is measured according to the scale setting ratio ruler on plate.
Application in the elongation ability identification of direct-sowing dry rice mesocotyl through the invention, can significantly shorten test week
Phase improves mesocotyl phenotypic evaluation efficiency, reduces test error caused by human factor.Meanwhile compared with other methods, this hair
The bright repeatability and stability for substantially increasing direct-sowing dry rice mesocotyl elongation ability qualification result.Three assessed at the same time
In the methods of the other identification direct-sowing dry rice mesocotyls elongation abilities of kind, related coefficient has been up to 0.88 between different batches
(having used the loam from field), and the present invention can be such that the related coefficient between different batches test for identification result reaches
0.95, and commercialized Nutrition Soil formula is clear, different researchers can replicate completely method.
Detailed description of the invention
Fig. 1 is the phenotypic variation situation of four kinds of lower 208 rice varieties of different training methods.
Fig. 2 is phase of the mesocotyl length of 208 parts of rice materials obtained under different training methods between repeating twice
Guan Xing.
Fig. 3 is situation of the mesocotyl length range of variation situation of MAGIC group in test twice.
Fig. 4 is correlation of the mesocotyl length in test twice repeats.
Specific embodiment
The present invention is described in detail combined with specific embodiments below.Following embodiment will be helpful to the technology of this field
Personnel further understand the present invention, but the invention is not limited in any way.It should be pointed out that the ordinary skill of this field
For personnel, without departing from the inventive concept of the premise, various modifications and improvements can be made.These belong to the present invention
Protection scope.
Embodiment 1
With water culture, sand culture method, earth culture method (field loam) and Nutrition Soil (German K board-TS1 type) training method are identified together respectively
The mesocotyl of one Rice Population (208 parts of rice germplasms that International Rice is introduced) extends ability.Every kind of cultural method setting 2
A independent repetition.The use of the specification of hole tray is 54cm (length) × 28cm (width) × 11cm (height), 50 caves.
A. in water culture, it is laid in the bottom in every cave with gauze, chooses 15 full seeds and is laid on gauze,
Until hole tray is placed in pallet and is filled to the position that the water surface did not had seed 1cm in pallet, then with masking foil by hole tray
With pallet sealing, shading, finally the hole tray being sealed is placed in 30 DEG C of phjytotron, culture is measured after 7 days with ruler
Mesocotyl length.
B. sand culture method: before sowing, each cave in hole tray is filled at 6cm at the top of hole tray with sand, and hole tray is placed in
Equipped in husky pallet, sand depth is 3cm.Full dry seeds 15 are equally chosen, cave bottom is laid in, then with husky covering
Seed in hole tray is extremely concordant at the top of hole tray, and depth of planting is made to reach 6cm, is then watered by hole tray, until bottom tray water
Divide saturation and without bright water.The weight and label that each pallet and hole tray are recorded after watering, every moisturizing in 24 hours once to label
Weight.Finally hole tray is placed in the phjytotron that temperature is 30 DEG C, samples after Seed germination and emergence, measured with ruler
Mesocotyl length.
C. earth culture method before sowing, each cave in hole tray is filled at 6cm at the top of hole tray with soil, and hole tray is placed in
In pallet equipped with soil, native depth is 3cm.Full dry seeds 15 are equally chosen, cave bottom is laid in, then with soil covering
Seed in hole tray is extremely concordant at the top of hole tray, and depth of planting is made to reach 6cm, is then watered by hole tray, until bottom tray water
Divide saturation and without bright water.The weight and label that each pallet and hole tray are recorded after watering, every moisturizing in 24 hours once to label
Weight.Finally, hole tray is placed in the phjytotron that temperature is 30 DEG C, samples after Seed germination and emergence, surveyed with ruler
Determine mesocotyl length.
D. nutrition earth culture method before sowing, each cave in hole tray is filled at 6cm at the top of hole tray with Nutrition Soil, and will
Hole tray is placed in the pallet equipped with Nutrition Soil, and native depth is 3cm (or 500g).Full dry seeds 15 are equally chosen, are put down
It is laid on cave bottom, it is then extremely concordant at the top of hole tray with the seed in Nutrition Soil covering hole tray, so that depth of planting is reached 6cm, then
It is watered by hole tray, being subject in lower opening has water leakage, then hole tray is taken out from pallet, with tap water by the nutrition in pallet
Soil pours to saturation, and hole tray is put back in pallet, is 2000g by the overall control of watering, hereafter, with the Nutrition Soil smashed by cave
Nutrition Soil height in disk is mended to concordant with pocket.The weight and label of each pallet and hole tray are recorded after watering, it is small every 24
When moisturizing once to label weight.Finally, it is 35 DEG C that hole tray, which is placed on temperature, relative humidity is the dark culture of 50%-60%
In room.Observation rice seedling situation daily, and emergence sequence is recorded, the 3rd day after first kind is emerged samples, takes pictures,
The mesocotyl length of different rice varieties in the cultivating system is quickly measured using the fixed method of image.
Test result analysis:
Rice mesocotyl phenotypic variation under different training methods.
Fig. 1 is the phenotypic variation situation of four kinds of lower 208 rice varieties of different training methods, and abscissa represents each culture
Independent experiment twice under mode repeats.As shown in Figure 1, the phenotypic variation significant difference under different training methods, this 208 parts of water
The range of variation of rice material mesocotyl is 0-4.8cm (Figure 1A) in earth culture, is 0-2.1cm in sand culture, except extremely different value
(Figure 1B) is 0-4.0cm (Fig. 1 C) in water planting, and in nutrition earth culture, this range of variation is maximum, is 0-5.1cm (figure
1D).Biggish phenotypic variation is the basis of genetic analysis, therefore from the angle analysis of mesocotyl length variation range, this patent
Proposed in nutrition earth culture method be better than other methods.
Fig. 2 reflects the mesocotyl length of 208 parts of rice materials obtained under different training methods between repeating twice
Correlation.As seen from the figure, correlation of the mesocotyl length between two repetitions is poor under the conditions of sand culture and water planting, R2Respectively
For 0.5150 (Fig. 2 B) and 0.5966 (Fig. 2 C), in earth culture preferably, R2Reach 0.7858 (Fig. 2A), and in Nutrition Soil culture
Under the conditions of R20.9133 (Fig. 2 D) can be reached, and slope k value, closest to 1, this shows in four kinds of mesocotyl length identification methods
The repeatability for the nutrition earth culture method that this patent is proposed is best with stability.
Embodiment 2
Utilize variation of the nutrition earth culture method detection mesocotyl length in 395 parts of MAGIC Rice Populations, concrete operations step
Suddenly identical as above-mentioned nutrition earth culture method.
Test result analysis:
The mesocotyl length range of variation of MAGIC group is 0-4.8cm, and is evenly distributed, continuously, this result is twice
In test repetition almost the same (Fig. 3).At the same time, correlation higher (R of the mesocotyl length in test twice repeats2=
0.8459) correlation results in (Fig. 4), with embodiment 11 are almost the same.
Specific embodiments of the present invention are described above.It is to be appreciated that the invention is not limited to above-mentioned
Particular implementation, those skilled in the art can make a variety of changes or modify within the scope of the claims, this not shadow
Ring substantive content of the invention.In the absence of conflict, the feature in embodiments herein and embodiment can any phase
Mutually combination.
Claims (1)
1. a kind of identification method of direct-sowing dry rice mesocotyl elongation ability, characterized by the following steps:
S1, Nutrition Soil was smashed to 5 meshes, stirred evenly spare；
S2, it is packed into the Nutrition Soil that 500g is smashed in pallet, paves, is compacted, hole tray is placed in pallet, in each cave of hole tray
It is middle to insert a certain amount of Nutrition Soil smashed and compacting, make the difference in height 6cm of native face and pocket；
S3, the full uniform and intact glume rice paddy seed of selection, are sowed, one, every cave kind, each kind is broadcast in sequence
15, and 15 seeds are evenly distributed on cave bottom；
The Nutrition Soil smashed uniformly is covered after the completion of S4, all material sowing, until native face is concordant with hole tray mouth, weighs pallet, cave
The quality summation of disk and Nutrition Soil therein and seed, is denoted as W1；
S5, the Nutrition Soil in hole tray is irrigated with the mode of spray, being subject in lower opening has water leakage, then by hole tray from pallet
It takes out, the Nutrition Soil in pallet is poured to saturation with tap water, and hole tray is put back in pallet, the overall control of watering is existed
Hereafter 2000g is mended the Nutrition Soil height in hole tray to concordant with pocket with the Nutrition Soil smashed, weighing gross weight is W2；
S6, above-mentioned pallet and hole tray are placed in together temperature be 35 DEG C, relative humidity be 50%-60% dark culture room in,
Every 24 hours, spray was primary, controlled the water of spray, and the gross weight after making water drenching is maintained at W2 level；
S7, emergence is observed daily, record the sequencing of different cultivars emergence, the 3rd day after first kind is emerged
Mesocotyl length is measured by sampling；Rice shoot is taken out from hole tray in order, is rinsed well, growing way in every part of material is uniformly young
Seedling is successively lain in on graduated plate, and is taken pictures, should be noted when taking pictures camera position in the surface of plate, highly with
Subject to entire plate is put into view-finder, and with tripod fixed camera position；After the completion of all kinds are successively taken pictures, use
Image J software measures the mesocotyl length of each kind according to the scale setting ratio ruler on plate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910719624.1A CN110326503B (en) | 2019-08-06 | 2019-08-06 | Identification method for elongation capability of hypocotyl in dry direct-seeded rice |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910719624.1A CN110326503B (en) | 2019-08-06 | 2019-08-06 | Identification method for elongation capability of hypocotyl in dry direct-seeded rice |
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| CN110326503A true CN110326503A (en) | 2019-10-15 |
| CN110326503B CN110326503B (en) | 2021-04-13 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111802203A (en) * | 2020-06-02 | 2020-10-23 | 佛山科学技术学院 | Method for evaluating pea growth condition |
| CN116574829A (en) * | 2023-04-03 | 2023-08-11 | 中国农业科学院作物科学研究所 | Molecular marker linked with rice mesocotyl elongation gene qML3 and application thereof |
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| CN101103699A (en) * | 2006-07-10 | 2008-01-16 | 曹立勇 | Method for selecting and breeding new variety of direct-seeded rice with mesocotyl length as index |
| CN106212136A (en) * | 2016-07-28 | 2016-12-14 | 四川省农业科学院水稻高粱研究所 | A kind of rice varieties top soil ability authentication method |
| CN107549010A (en) * | 2017-09-28 | 2018-01-09 | 吉林省农业科学院 | A kind of method of the high sorghum variety of simple and quick screening emergence rate |
| CN208657298U (en) * | 2018-08-29 | 2019-03-29 | 甘肃农业大学 | A kind of seed top soil capability determining instrument |
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2019
- 2019-08-06 CN CN201910719624.1A patent/CN110326503B/en active Active
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|---|---|---|---|---|
| CN101103699A (en) * | 2006-07-10 | 2008-01-16 | 曹立勇 | Method for selecting and breeding new variety of direct-seeded rice with mesocotyl length as index |
| CN106212136A (en) * | 2016-07-28 | 2016-12-14 | 四川省农业科学院水稻高粱研究所 | A kind of rice varieties top soil ability authentication method |
| CN107549010A (en) * | 2017-09-28 | 2018-01-09 | 吉林省农业科学院 | A kind of method of the high sorghum variety of simple and quick screening emergence rate |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111802203A (en) * | 2020-06-02 | 2020-10-23 | 佛山科学技术学院 | Method for evaluating pea growth condition |
| CN116574829A (en) * | 2023-04-03 | 2023-08-11 | 中国农业科学院作物科学研究所 | Molecular marker linked with rice mesocotyl elongation gene qML3 and application thereof |
| CN116574829B (en) * | 2023-04-03 | 2024-03-12 | 中国农业科学院作物科学研究所 | Molecular marker linked with rice mesocotyl elongation gene qML3 and application thereof |
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| CN110326503B (en) | 2021-04-13 |
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