CN107549010A - A kind of method of the high sorghum variety of simple and quick screening emergence rate - Google Patents
A kind of method of the high sorghum variety of simple and quick screening emergence rate Download PDFInfo
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- CN107549010A CN107549010A CN201710899205.1A CN201710899205A CN107549010A CN 107549010 A CN107549010 A CN 107549010A CN 201710899205 A CN201710899205 A CN 201710899205A CN 107549010 A CN107549010 A CN 107549010A
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Abstract
The invention provides a kind of method of the high sorghum variety of simple and quick screening emergence rate, specially a kind of method of simple, quickly one Sorghum Germplasm of measure, parent or cenospecies mesocotyl extended length, this method utilizes the seed that vernalization is cultivated in the culture box water planting with filter paper and dark, the sorghum mesocotyl obtained, it is erectility, facilitate follow-up measurement work, or directly it can be compared by visual observations, completely left out measurement work, greatly improve the operating efficiency of heavy screening measurement sorghum mesocotyl actual at present；In addition, cultivating the method for sorghum mesocotyl in water planting and dark, the cycle is short, simple to operate, so as to efficiently judge or compare the relative bud ratio situation of the sorghum variety of different genetic backgrounds.
Description
Technical field
The invention belongs to Crops ' Genetic Breeding field, and in particular to a kind of side for screening the high sorghum variety of emergence rate
Method.
Background technology
Low sorghum seeds emergence rate is frequent produced problem in production, and larger loss is often caused to agricultural production.
The reason for causing sorghum seeds emergence rate low has a lot, such as climatic factor (chilling injury), tillage control measure, depth of planting, disease
Insect pest etc., but the maximum potential of sorghum seeds Emerging ability is that gene determine, in rhizome between sorghum different genotype
There is pole significant difference in length, emergence number of days and emergence rate, the high sorghum variety of screening emergence rate, sorghum is produced and plants industry and height
Fine strain of millet genetic breeding research is very important work, therefore, invents a kind of high sorghum of screening emergence rate that can be simple and quick
The method of kind, it can greatly improve sorghum production and plant industry and the operating efficiency of sorghum genetic breeding research.
In gramineous crop, the mesocotyl research to rice, corn shows, mesocotyl and its seed top soil Emerging ability
It is relevant.The material of mesocotyl length, wherein Embryo stem extension are obvious, and speed of emergence is fast, and emergence rate is high；And the material that mesocotyl is short, go out
Seedling speed is slow, and emergence rate is low.The plumular axis of sorghum can divide into hypocotyl, middle embryo as the plumular axis of other gramineous crops
Axle and epicotyl.Sprouted in sorghum seeds to during as seedling, plumule is delivered to soil by mesocotyl elongation together with plumule
Table, therefore, mesocotyl are to contact primary root and the passage of seedling.Research shows that the elongation ability of sorghum mesocotyl is different sowings
An important factor for advanced treating emergence rate changes.Therefore, there is positive correlation, institute with emergence rate for the elongation ability of sorghum mesocotyl
With the sorghum relatively long by screening sorghum mesocotyl, you can to filter out the high sorghum variety of emergence rate.
The content of the invention
It is an object of the present invention to provide a kind of method for screening the high sorghum variety of emergence rate, methods described includes：
Using culture apparatus culture sorghum seeds, the bottom of the culture apparatus is covered with filter paper.
Specifically, the filter paper includes more than 1 layer.
Again specifically, the filter paper is 2 layers.
Specifically, the culture is included in light culture in water.
The temperature of the light culture includes 25 DEG C.
The culture apparatus includes culture box with cover；The culture box includes the culture box diameter 9cm, high 15cm.
The light culture includes the culture apparatus being placed in incubator carrying out light culture.
The light culture is included in the culture apparatus box plus water.
The light culture includes culture 5 days.
Specifically, before the light culture, in addition to：Seed disinfection；And/or seed is sprouted.
The seed disinfection includes：Seed is soaked with mercuric chloride solution, then rinsed seed with water.
Specifically, the mercuric chloride solution includes the mercuric chloride solution that concentration is 1g/L；The immersion includes immersion
10min；Described rinse includes using deionized water rinsing again after being rinsed with running water；The flushing includes rinsing 3 times.
The seed, which is sprouted, to be included：Seed is placed in water 30 DEG C of light culture 24h.
Also include specifically, the seed is sprouted, seed is placed in the 9cm culture dishes with cover for being covered with 2 layers of filter paper, adds water
10ml, it is put into incubator, 30 DEG C of temperature, light culture 24h.
It is a further object to provide a kind of culture apparatus for screening the high sorghum variety of emergence rate, the culture
The bottom of device is covered with filter paper.
Specifically, the filter paper includes more than 1 layer.
Again specifically, the filter paper is 2 layers.
The culture apparatus includes culture box with cover；The culture box includes the culture box diameter 9cm, high 15cm.
A further object is for the present invention provides a kind of method for cultivating sorghum mesocotyl, and methods described includes：Using training
Device culture sorghum seeds are supported, the bottom of the culture apparatus is covered with filter paper.
Specifically, the filter paper includes more than 1 layer.
Again specifically, the filter paper is 2 layers.
Specifically, the culture is included in light culture in water.
The temperature of the light culture includes 25 DEG C.
The culture apparatus includes culture box with cover.The culture box includes the culture box diameter 9cm, high 15cm.
The light culture includes the culture apparatus being placed in incubator carrying out light culture.
The light culture is included in the culture apparatus box plus water.
The light culture includes culture 5 days.
Specifically, before the light culture, in addition to：Seed disinfection；And/or seed is sprouted.
The seed disinfection includes：Seed is soaked with mercuric chloride solution, then rinsed seed with water.
Specifically, the mercuric chloride solution includes the mercuric chloride solution that concentration is 1g/L；The immersion includes immersion
10min；Described rinse includes using deionized water rinsing again after being rinsed with running water；The flushing includes rinsing 3 times.
The seed, which is sprouted, to be included：Seed is placed in water 30 DEG C of light culture 24h.
Also include specifically, the seed is sprouted, seed is placed in the 9cm culture dishes with cover for being covered with 2 layers of filter paper, adds water
10ml, it is put into incubator, 30 DEG C of temperature, light culture 24h.
It is also another object of the present invention to provide the application of any methods described of the present invention, any described device of the present invention.
Specifically, the application is included in following 1) -6) it is at least one in application：
1) application in sorghum is cultivated；
2) application in the Related product for preparing the high sorghum variety of screening emergence rate；
3) application in the Related product for preparing culture sorghum；
4) application in sorghum mesocotyl is cultivated；
5) application in the Related product for preparing culture sorghum mesocotyl；
6) in the application of the high sorghum variety of screening emergence rate.
The present invention cultivates mesocotyl in the dark by water planting, can simply, quickly determine Sorghum Germplasm, a parent
Or the extended length of cenospecies mesocotyl, according to the extended length of mesocotyl and the positively related principle of bud ratio, so as to efficient
Judge or compare different genetic backgrounds sorghum variety relative bud ratio situation.
The main beneficial effect of the method for the invention includes：
1st, the method that water planting provided by the invention cultivates sorghum mesocotyl in the dark, the cycle is short, simple to operate, is a kind of
Simple, fast and efficient method.
2nd, the sorghum mesocotyl turned out using the culture box provided by the invention with filter paper, it is erectility, convenient
Follow-up measurement work, or directly can be compared by visual observations, completely left out measurement work, greatly improve current reality
The operating efficiency of heavy screening measurement sorghum mesocotyl.
Embodiment
Experiment or breeding method used in following embodiments are conventional method unless otherwise specified.
Material used, reagent etc., unless otherwise specified, are commercially obtained in following embodiments.
Embodiment 1, the method for cultivating sorghum mesocotyl
1. vernalization
Every part of material selection is in the same size, 100, the seed of full seed, 1g/L mercuric chloride solution sterilization 10min, so
Running water rinses 3 times afterwards, deionized water rinsing 3 times.Uniformly fitly it is emitted in the 9cm culture dishes with cover for being covered with 2 layers of filter paper,
50 seeds of each culture dish, add water 10ml, 2 culture dishes of every part of material.It is put into incubator, 30 DEG C of temperature, light culture
24h。
2. cultivate mesocotyl
Light culture 24h culture dish is taken out, every part of material selection has just revealed seed sharp, of the same size and has been put into culture with cover
In box, culture box diameter 9cm, high 15cm, 2 layers of filter paper are spread in culture cassette bottom portion, the seed of 15-20 grains vernalization is placed on filter
On paper, each box of cultivating adds water 10ml.3 repetitions of every part of material.It is put into incubator, 25 DEG C of temperature, light culture 5d.
3. measure mesocotyl length
Culture box light culture 5d after taking-up will be placed in incubator, and each box of cultivating takes out 10 seedling at random, and it is
Mesocotyl is erectility, cuts mesocotyl, measures the length of mesocotyl；Or it can directly pass through the more different sorghums of visual observations
The length of the mesocotyl of kind.
The relative bud ratio situation of different sorghum varieties, the longer sorghum product of mesocotyl are judged according to the length of mesocotyl
Kind, its bud ratio is higher.
Claims (10)
- A kind of 1. method for screening the high sorghum variety of emergence rate, it is characterised in that methods described includes：Trained using culture apparatus Sorghum seeds are supported, the bottom of the culture apparatus is covered with filter paper.
- 2. according to the method for claim 1, it is characterised in that：The filter paper includes more than 1 layer；The culture apparatus includes Culture box with cover.
- 3. according to the method described in claim 1 and/or 2, it is characterised in that：The culture is included in light culture in water.
- 4. according to any described method of claim 1/2 and/or 3, it is characterised in that：Before the light culture, in addition to：Kind Son sterilization；And/or seed is sprouted；The seed disinfection includes：Seed is soaked with mercuric chloride solution, then rinsed seed with water；The seed, which is sprouted, to be included：Seed is placed in water 30 DEG C of light culture 24h.
- 5. a kind of culture apparatus for screening the high sorghum variety of emergence rate, it is characterised in that the bottom of the culture apparatus is covered with Filter paper.
- 6. culture apparatus according to claim 5, it is characterised in that：The filter paper includes more than 1 layer；The culture apparatus Including culture box with cover.
- A kind of 7. method for cultivating sorghum mesocotyl, it is characterised in that methods described includes：Using culture apparatus culture sorghum kind Son, the bottom of the culture apparatus are covered with filter paper.
- 8. according to the method for claim 7, it is characterised in that：The filter paper includes more than 1 layer；The culture apparatus includes Culture box with cover.
- 9. according to the method described in claim 7 and/or 8, it is characterised in that：The culture is included in light culture in water.
- 10. any methods describeds of claim 1-4, any described devices of claim 5-6, any sides of claim 7-9 The application of method, it is characterised in that the application is included in following 1) -6) it is at least one in application：1) application in sorghum is cultivated；2) application in the Related product for preparing the high sorghum variety of screening emergence rate；3) application in the Related product for preparing culture sorghum；4) application in sorghum mesocotyl is cultivated；5) application in the Related product for preparing culture sorghum mesocotyl；6) in the application of the high sorghum variety of screening emergence rate.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108575183A (en) * | 2018-05-12 | 2018-09-28 | 山东省农业科学院作物研究所 | A kind of sorghum seedling stage root-planting method |
| CN110326503A (en) * | 2019-08-06 | 2019-10-15 | 中国农业科学院深圳农业基因组研究所 | A kind of identification method of direct-sowing dry rice mesocotyl elongation ability |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105993878A (en) * | 2016-05-19 | 2016-10-12 | 浙江省农业科学院 | High-throughput method for screening sorghum varieties suitable for deep sowing |
| CN106576966A (en) * | 2016-12-13 | 2017-04-26 | 山西省农业科学院高粱研究所 | Method of identifying salt tolerance of grain sorghum |
| CN106879445A (en) * | 2017-04-06 | 2017-06-23 | 山东省农业科学院作物研究所 | A kind of method of Rapid identification and screening sorghum germination period salt tolerance material |
-
2017
- 2017-09-28 CN CN201710899205.1A patent/CN107549010A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105993878A (en) * | 2016-05-19 | 2016-10-12 | 浙江省农业科学院 | High-throughput method for screening sorghum varieties suitable for deep sowing |
| CN106576966A (en) * | 2016-12-13 | 2017-04-26 | 山西省农业科学院高粱研究所 | Method of identifying salt tolerance of grain sorghum |
| CN106879445A (en) * | 2017-04-06 | 2017-06-23 | 山东省农业科学院作物研究所 | A kind of method of Rapid identification and screening sorghum germination period salt tolerance material |
Non-Patent Citations (1)
| Title |
|---|
| 陈冰嬬等: "不同类型高粱中胚轴伸长特性的研究", 《作物杂志》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108575183A (en) * | 2018-05-12 | 2018-09-28 | 山东省农业科学院作物研究所 | A kind of sorghum seedling stage root-planting method |
| CN108575183B (en) * | 2018-05-12 | 2021-04-13 | 山东省农业科学院作物研究所 | Sorghum seedling stage rooting method |
| CN110326503A (en) * | 2019-08-06 | 2019-10-15 | 中国农业科学院深圳农业基因组研究所 | A kind of identification method of direct-sowing dry rice mesocotyl elongation ability |
| CN110326503B (en) * | 2019-08-06 | 2021-04-13 | 中国农业科学院深圳农业基因组研究所 | Identification method for elongation capability of hypocotyl in dry direct-seeded rice |
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