CN105993878A - High-throughput method for screening sorghum varieties suitable for deep sowing - Google Patents
High-throughput method for screening sorghum varieties suitable for deep sowing Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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Abstract
The invention discloses a high-throughput method for screening sorghum varieties suitable for deep sowing. The method comprises the following steps: (1) a hydroponic device is prepared, wherein the hydroponic device is composed of a container, a foamed plastic board and a mesh; round through holes with a same pore size are uniformly provided on the foamed plastic board; the mesh is fixed on the bottom of the round through holes; the foamed plastic plate is placed in the container filled with water; (2) sowing and seedling culture are carried out, wherein sorghum seeds to be tested are placed in the round through holes; dark culture is carried out for 6.5-7.5 days under a constant temperature of 28 +/-0.5 DEG C; and (3) mesocotyl lengths of the obtained sorghum seedlings are determined. Under a same culture period, the sorghum variety with longer mesocotyl length is suitable for deep sowing.
Description
Technical field
The invention belongs to Sorghum vulgare Pers. breeding of new variety field, be specifically related to a kind of high flux screening and be suitable for seed deeplying the technology of sorghum variety.
Background technology
China Sorghum vulgare Pers. main producing region is distributed in northeast, North China, the Huaibei, southwest and northwest etc. and relies on hilly ground or the Plain of natural precipitation
Nonirrigated farmland.If there is arid in sowing to seeding stage, use the conventional seed planting degree of depth (generally about 5cm), will be because of topsoil
Moisture deficit, seed is difficult to sprout and emerge, Uneven seedlings occurs, affects field Seedling number, causes the underproduction.Sowed deep (super
Cross 9cm), add seedling and pass the thickness of the layer and go out soil resistance, bud scale is unearthed difficulty, seedling in soil foliation yellow and
Dead.It addition, sowed deep, soil air is rare, and in the case of, moisture higher at the soil moisture is more, seed is breathed tired
Difficulty, vitality weakens, forfeiture germinating capacity (Yang Zanlin, 1963；Lu Shanqing, 1999).
Different cultivars is different to the reaction of depth of planting.Deep seeding-tolerant hybrid is newly discovered as one and has been applied to cultivate excellent
The germ plasm resource of kind, it is possible to effectively utilize deep subsoil moisture, it is to avoid the arid impact not enough with soil table moisture in the soil of endogenous cause of ill in sowing time,
Guarantee normal seed germination (Schillinger et al., 1998；Mahdi et al.,1998).Additionally, Deep seeding-tolerant hybrid can also pass through
Increase plant root density in deep soil, improve the Analysing Root Characters such as root/shoot ratio, strengthen its drought resistance and lodging tolerance,
Final improve resistance to broadcast kind deeply yield (Ehsanuliah et al., 1999；Dong Cunji etc., 1994；King is the most flourish, and 1994；Clock
Change honor etc., 1998).
Oneself has research to confirm at present, and the elongation of mesocotyl can significantly improve cereal crop emergence rate under the conditions of earthing is relatively deep
And seedling quality (Dungan 1950；Hoshikaw 1969；Biswas et al.,1997；Chung 2010；Wang Nan etc., 2007).
The sorghum variety that mesocotyl substantially extends has following numerous advantage: the elongation ability of mesocotyl is strong, emerges all right；At soil table
During moisture in the soil deficiency by seed deeply can allow neutron absorption deep layer moisten moisture relend help mesocotyl elongation promote emerge；Raw at middle embryo
Adventitious root intensive on axle can ensure that initial tens days in the absorption of growth of seedling desired nutritional material and transport；Of short duration on a small quantity
After rainfall, the moisture penetrating into topsoil is easy to be absorbed by raw adventitious root on mesocotyl；Before emerging after broadcasting heavy rain or
Flood irrigation can cause soil compaction, can buffer soil compaction making emergence difficult by seed deeplying.
Existing cereal crop mesocotyl elongation ability authentication method mainly uses earth culture identification method.The shortcoming of the method is needs
Manually fetch earth, sieve, mix, fill soil, water, the series of steps such as earthing, the error of each link can cause experiment
Result is inaccurate.In addition, also need before measuring mesocotyl to clean seedling.In a word, earth culture identification method is used to there is operation many,
Workload is big, longevity of service, repeatable poor, the shortcomings such as Large-scale Screening efficiency is low.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of method being applicable to Large-scale Screening Sorghum vulgare Pers. mesocotyl elongation ability, uses
Water planting qualification result replaces traditional earth culture qualification result.This invention simplifies screening step, eliminate because personal error is to qualification
The impact of result, improves the accuracy of qualification result, and the selection result is reproducible, decreases experimentation cost simultaneously, shortens
Qualification time, can repeatedly screen in 1 year.
In order to solve above-mentioned technical problem, the present invention provides a kind of method that high flux screening is suitable for seed deeplying sorghum variety, including with
Lower step:
1), hydroponic device is set:
This hydroponic device is made up of container, plastic foamboard and gauze；
It is uniformly arranged the manhole that aperture is consistent on plastic foamboard, is fixed with gauze in the bottom of manhole；
Plastic foamboard is placed in equipped with in the container of water；
2), sowing and seedling are cultivated:
The seed of Sorghum vulgare Pers. to be measured is loaded manhole, under the constant temperature of 28 ± 0.5 DEG C, carries out dark culturing, ambient humidity (phase
To humidity) it is 75% ± 2%, make sorghum seeds naturally sprout and emerge, incubation time is 6.5～7.5 days (preferably 7 days), obtains high
Fine strain of millet Seedling；
3), the mensuration of mesocotyl:
Step 2) gained Sorghum Seedlings measure mesocotyl length；
Under identical incubation time, the Sorghum vulgare Pers. to be measured of mesocotyl length is for relatively to be suitable for seed deeplying sorghum variety.
It is suitable for seed deeplying the improvement of the method for sorghum variety as the high flux screening of the present invention:
Described step 2) in, the Sorghum vulgare Pers. to be measured of each kind arranges 3 repetitions, 30 seeds of each repeat sowing；
Described step 3) in, from each repetition, randomly choose 10 strain Sorghum Seedlings carry out the mensuration of mesocotyl length；Take 30 plant height fine strains of millet
The meansigma methods of the mesocotyl of Seedling is as the mesocotyl length of this sorghum variety to be measured.
It is suitable for seed deeplying the further improvements in methods of sorghum variety as the high flux screening of the present invention:
A diameter of 3.5 of manhole on described plastic foamboard～4.5cm (preferably 4cm)；
The height of described manhole is 16～18mm (preferably 17mm).
High flux screening as the present invention is suitable for seed deeplying the further improvements in methods of sorghum variety: the specification of described gauze is 16
Mesh.
It is suitable for seed deeplying the further improvements in methods of sorghum variety as the high flux screening of the present invention:
Described step 3) in, as the mesocotyl length-depth of planting >=2cm of Sorghum vulgare Pers. to be measured, it is determined that this Sorghum vulgare Pers. to be measured belongs to suitable
Close the sorghum variety of this depth of planting.
High flux screening as the present invention is suitable for seed deeplying the further improvements in methods of sorghum variety: when the mesocotyl of Sorghum vulgare Pers. to be measured
During length >=12cm, it is determined that this Sorghum vulgare Pers. to be measured belongs to the sorghum variety that applicable depth of planting is 10cm；
As the mesocotyl length >=22cm of Sorghum vulgare Pers. to be measured, it is determined that this Sorghum vulgare Pers. to be measured belongs to the Sorghum vulgare Pers. that applicable depth of planting is 20cm
Kind.
In the present invention, the mensuration of mesocotyl length belongs to routine techniques, and mesocotyl length refers to that radicle attachment region is to plumule base portion
Between length.
Technical scheme specifically can be as follows:
(1) Sorghum vulgare Pers. hydroponic device:
It is made up of plastic box, plastic foamboard and gauze.The specification of plastic box is 630 × 413 × 150mm, foam
The specification of plate is 580 × 395 × 17mm, it is uniform-distribution with the circular hole of 77 diameter 4cm, gauze specification be 580 ×
395mm, 16 mesh.Gauze need to be fixed on the bottom of cystosepiment with glass cement.
(2) sowing and seedling are cultivated:
Each Fruit variety is full, 90, unabroken seed, is divided into three equal parts.By planting seed at the cystosepiment adhering to gauze
In circular hole, experiment sets 3 repetition, each repeats one circular hole of correspondence (that is, each repetition 30 strain).After each kind is broadcast, then
Cystosepiment is swum in the plastic box filling 28L tap water, be placed on the phjytotron of temperature controllable and humidity.People
The temperature of work climatic chamber is set as 28 DEG C, dark culturing, allows it naturally sprout and emerges.
(3) mensuration of mesocotyl
After 1 week, terminate dark culturing.The each repetition of each kind selects 10 strain seedling to measure mesocotyl length.
(4) qualification result reliability demonstration
According to the data of the mesocotyl length that water culture measures, select the sorghum variety of long mesocotyl and brachymedial plumular axis to carry out two kinds and cover
Soil depth experiments, investigates emergence rate and mesocotyl length, the effectiveness of checking water culture screening.
The invention provides a kind of high flux screening and be suitable for seed deeplying the technology of sorghum variety, use water culture to cultivate under dark condition
Sorghum seeds, with mesocotyl elongation characteristics as index so that filter out applicable seed deeply kind and be resistant to when nonirrigated farmland is live
More than 20cm depth of planting, and can normally emerge.
The practicality of the water planting authentication method of the present invention is: simple operating steps, only need to be by planting seed at the bubble adhering to gauze
In foam plate circular hole, then swim in the plastic box filling tap water, dark culturing, and without moisturizing in incubation,
Cultivation can directly take seedling and measure mesocotyl length after terminating；Experimental period significantly shortens, can be from being seeded into that character investigation terminates
Complete in 10 days；Screening efficiency is high, once can identify thousands of parts of sorghum varieties the artificial climate indoor of 40 square metres simultaneously；
Result is reproducible, it is provided that consistent growing environment, can effectively get rid of in earth culture identification method because of earthing and water etc. uneven and draw
The experimental error risen；Result reliability is high, and between kind, mesocotyl elongation difference of ability is obvious, with the knot of potted plant earthing experimental verification
Fruit is consistent.The method is seed deeplyed CHARACTERISTICS IDENTIFICATION sorghum variety resource and breeding intermediate materials resistance to, resistance to seed deeplyed in genetic development research
Application potential is bigger.
In sum, the present invention is a kind of water planting authentication method being applicable to Large-scale Screening Sorghum vulgare Pers. mesocotyl elongation ability, the party
Method have easy and simple to handle, the cycle is short, when saving of labor saves, reproducible, can repeatedly carry out in 1 year, the many such as sampling operation is convenient
Advantage.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is further detailed explanation, but they are not limitation of the invention.
In following example, the specification of plastic box is 630 × 413 × 150mm, the specification of cystosepiment is 580 × 395 ×
17mm, it is uniform-distribution with the circular hole of 77 diameter 4cm, and gauze specification is 580 × 395mm, 16 mesh.Gauze needs in advance
The bottom of cystosepiment it is fixed on glass cement.The cystosepiment that bottom is fixed with gauze swims in the plastics turnover filling 28L tap water
In case.It is placed on the phjytotron of temperature controllable and humidity.
Embodiment 1: the resistance to technology seed deeplying sorghum variety of a kind of high flux screening, for 152 parts from the most different local varieties
The resistance to screening seed deeplying material of resource.Planting seed is in the cystosepiment circular hole of attachment gauze, then is swum in by cystosepiment and fill 28L
In the plastic box of tap water.Experiment is carried out in the controlled environment chamber.Phjytotron is set according to Sorghum vulgare Pers. suitable growth temperature
It is 28 DEG C, owing to Turnover Box containing a lot of tap water, need not artificially humidify and dehumidify, and the constant humidity of 75% can be kept,
Dark culturing 1 week (7 days).
Utilize this hydroponic device and ciltivating process, carry out the mensuration of mesocotyl length.Result shows interracial mesocotyl luffing
Between 5.2～25.2cm, difference reaches pole significant level.Specifically, little Bai grain, three generations Sorghum vulgare Pers., LN8125,10032,
DURA EL MEK, MN-55, LTR108, Btx623 are brachymedial plumular axis kind (mesocotyl length ＜ 10cm), broom broom corn millet,
Big black cap, upper well Sorghum vulgare Pers., hammer of beating a gong, BATHURST SUMAC are long mesocotyl kind (mesocotyl length ＞ 20cm).
The mesocotyl elongation ability of part variety source when table 1, water planting
Embodiment 2: material to be tested is 654, LTR108 and their hybridization after by simple grain pass obtain recombinant inbred lines
(F10, 244 strains), study the resistance to genetic development seed deeplying Sorghum vulgare Pers..Repeat embodiment 1: have a following difference:
Determine the mesocotyl length of parent and recombinant inbred lines.The mesocotyl of parent 654 and LTR108 grows 11.2 respectively ±
0.5cm and 5.0 ± 0.1cm, in colony, the mesocotyl length luffing of strain is between 6.3～18.6cm, with strain in colony between parent
Between difference reach pole significant level.Mesocotyl length approximate normal distribution in colony, show Inheritance of Quantitative Characters feature, be suitable for into
The resistance to genetic development research seed deeplying Sorghum vulgare Pers. of row.Utilizing this colony, inventor detects on the 1st, 5 and 8 chromosome affects mesocotyl
The quantitative trait locus of elongation, and potentiation allele comes from long mesocotyl parent 654.Result of study contributes to we have appreciated that
The resistance to genetic development seed deeplyed of Sorghum vulgare Pers..
Specifically, RIL-33, RIL-43, RIL-61, RIL-151, RIL-186, RIL-188 be brachymedial plumular axis kind (in
Plumular axis length ＜ 10cm), RIL-41, RIL-70, RIL-84, RIL-101, RIL-102, RIL-131 are long mesocotyl kind
(mesocotyl length ＞ 15cm).
The mesocotyl elongation ability of RIL part strain when table 2, water planting
Experiment 1, below resistance to seed deeply sorghum variety water planting for use the present invention and identify that the dependency with earth culture qualification result in seedling stage divides
Analysis.
1 materials and methods
1.1 material to be tested
1.1.1 152 parts of sorghum variety resources are come from
According to the result of above-described embodiment screening, inventor have selected the sorghum variety of mesocotyl short (＜ 10cm) under the conditions of water planting
Little Bai grain, three generations Sorghum vulgare Pers., LN8125,10032, DURA EL MEK, MN-55, LTR108, Btx623 and mesocotyl
Long the sorghum variety broom broom corn millet of (＞ 20cm), big black cap, upper well Sorghum vulgare Pers., hammer of beating a gong, BATHURST SUMAC conduct
Material to be tested.
1.1.2 the strain in recombinant inbred lines is come from
According to the result of above-described embodiment screening, inventor have selected strain RIL-33 of mesocotyl (＜ 10cm) under the conditions of water planting,
RIL-43, RIL-61, RIL-151, RIL-186, RIL-188 and strain RIL-41 of mesocotyl length (＞ 15cm), RIL-70,
RIL-84, RIL-101, RIL-102, RIL-131 and parent 654 and LTR108 are as material to be tested.
1.2 greenhouse earth cultures are identified
1.2.1 flowerpot earthing is identified
The soil of the arable layer fetched from field is smashed and sieves, then by weight being mixed into Nutrition Soil for 1:1, stir, dress
Enter in the plastic flowerpot of long 47cm, wide 33cm, high 26cm, for convenience of one layer of black shield of pad in sampling and measuring, flowerpot by soil
Separating with flowerpot, the most equidistant sowing comes from the brachymedial plumular axis filtered out in 152 parts of sorghum variety resources and long mesocotyl Sorghum vulgare Pers.
Kind, each kind is broadcast 30, is repeated for 3 times, then earthing 10cm and 20cm is deep, irrigates bottom water, sprouts under the conditions of 28 DEG C, respectively
After 15d and 20d, measure emergence rate deep for earthing 10cm and 20cm and mesocotyl is long.
1.2.2 nutritive cube earthing is identified
The soil of the arable layer fetched from field is smashed and sieves, then by weight being mixed into Nutrition Soil for 1:1, stir, dress
Enter in the plastic nutritional alms bowl of diameter 7cm, high 17cm.Material to be tested is the brachymedial plumular axis strain screened in recombinant inbred lines
System and long mesocotyl strain and parent 654 and LTR108, each kind is broadcast 30, is repeated for 3 times, then earthing 10cm is deep, waters and reveals the exact details
Water.Sprout under the conditions of 28 DEG C, measure emergence rate after 15d and mesocotyl is long.
2. result and analysis
2.1 flowerpot earthing identify emergence rate and mesocotyl elongation situation
Table 3, emergence rate deep for flowerpot earthing 10cm and mesocotyl elongation ability
Note: NA represents and causes shortage of data because of can not normally emerge, as follows.
Table 4, emergence rate deep for flowerpot earthing 20cm and mesocotyl elongation ability
2.2 nutritive cube earthing are identified and are emerged and mesocotyl elongation situation
Table 5, emergence rate deep for nutritive cube earthing 10cm and mesocotyl elongation ability
Remarks illustrate: at least to reach the emergence rate of 50%, just can be judged to be suitable for this depth of planting.Therefore, parent 654
It is judged as being not suitable for the depth of planting of 10cm.
Mesocotyl can only extend under dark condition, and its elongation ability is both relevant with the inherited character of kind, has with depth of planting again
Close.For from the kind in 152 parts of sorghum variety resources, when flowerpot earthing 10cm and 20cm is deep, this method filtering out
The short sorghum variety little Bai grain of mesocotyl, three generations Sorghum vulgare Pers., LN8125,10032, DURA EL MEK, MN-55, LTR108,
Btx623 can not normally be emerged, and the sorghum variety broom broom corn millet of mesocotyl length, big black cap, upper well Sorghum vulgare Pers., hammer of beating a gong,
BATHURST SUMAC emergence rate when earthing 10cm is deep reaches more than 65%, even if emergence rate can also be tieed up when earthing 20cm is deep
Hold between 50～60%.For from the strain in recombinant inbred lines, when nutritive cube earthing 10cm is deep, water planting condition
Short strain RIL-33, RIL-43, RIL-61, RIL-151, RIL-186, RIL-188 and the parent LTR108 of lower mesocotyl is the most not
Can normally emerge.And strain RIL-41 of mesocotyl length, the going out of RIL-70, RIL-84, RIL-101, RIL-102, RIL-131
Seedling rate is higher, is maintained between 73.3%～86.7%.Parent 654 is only 11.2cm because of its mesocotyl length under the conditions of water planting,
Only longer about 1cm than thickness of earth-fill cover, so emergence rate is significantly lower than the strain of mesocotyl length, only 30.0%, and also longer
Seedling the most small and weak, it is difficult to strain.Three test result indicate that, when the length of mesocotyl under the conditions of thickness of earth-fill cover exceedes water planting,
Variety source and strain all can not normally be emerged；When the length of mesocotyl under the conditions of thickness of earth-fill cover is significantly less than water planting, kind provides
Source and strain all have higher emergence rate, show the resistance to characteristic seed deeplyed.As can be seen here, the elongation ability of mesocotyl and emergence rate
In notable positive correlation, replace earth culture method really can reach to screen the resistance to effect seed deeplying sorghum variety with ciltivating process, and use water
The reflection of mesocotyl length that culture method is identified out to be that the maximum of variety source or strain is resistance to seed deeply potentiality, the degree of depth of actual sowing
Should reduce as one sees fit, at least few than the mesocotyl length under the conditions of water planting 2cm under above-mentioned earthing experiment condition.
Comparative example 1-1, making the condition of culture in embodiment 1 into 25 DEG C, incubation time makes 8 days into, at this temperature, by
In Turnover Box, fill tap water, need not artificially humidify and dehumidify, the constant humidity of 70% can be kept；Remaining is equal to embodiment
1。
The testing result of the kinds such as little Bai grain is as shown in table 6.
Mesocotyl elongation ability when table 6, comparative example 1-1 water planting
Comparative example 1-2, making the condition of culture in embodiment 1 into 30 DEG C, incubation time makes 6 days into, at this temperature, by
In Turnover Box, fill tap water, need not artificially humidify and dehumidify, the constant humidity of 80% can be kept；Remaining is equal to embodiment
1。
The testing result of the kinds such as little Bai grain is as shown in table 7 below.
Mesocotyl elongation ability when table 7, comparative example 1-2 water planting
According to above-mentioned comparative example 1-1, it has been found that water planting condition to be made into 25 DEG C, humidity 70%, during incubation time 8 days,
The judged result obtained is that broom broom corn millet, upper well Sorghum vulgare Pers. do not tolerate seed deeplying of 20cm, with the result of potted plant earthing experimental verification not
Symbol；That is, under this condition of culture, judged result generation deviation can be caused.
According to above-mentioned comparative example 1-2, it has been found that water planting condition to be made into 30 DEG C, humidity 80%, during incubation time 6 days,
The judged result obtained is that DURA EL MEK and Btx623 is resistant to the deep earthing of 10cm, and upper well Sorghum vulgare Pers. is not resistant to
20cm seed deeplys；It is not inconsistent with the result of potted plant earthing experimental verification；That is, under this condition of culture, judged result can be caused to occur
Deviation.
Comparative example 2-1, made the height of plastic foamboard in embodiment 1 into 10mm by 17mm, i.e. the height of manhole by
17mm makes 10mm into；Remaining is equal to embodiment 1.
The testing result of the kinds such as little Bai grain is as shown in table 8 below.
Mesocotyl elongation ability when table 8, comparative example 2-1 water planting
Comparative example 2-2, made the height of plastic foamboard in embodiment 1 into 30mm by 17mm, i.e. the height of manhole by
17mm makes 30mm into；Remaining is equal to embodiment 1.
The testing result of the kinds such as little Bai grain is as shown in table 9 below.
Mesocotyl elongation ability when table 9, comparative example 2-1 water planting
According to above-mentioned comparative example 2-1, it has been found that when the height (height of manhole) of plastic foamboard is set to 10mm,
The judged result obtained is, broom broom corn millet and BATHURST SUMAC are not resistant to seed deeplying of 20cm；Real with potted plant earthing
The result of checking is not inconsistent；That is, under this condition of culture, judged result generation deviation can be caused.
According to above-mentioned comparative example 2-2, it has been found that when the height (height of manhole) of plastic foamboard is set to 30mm,
The judged result obtained is, big black cap and upper well Sorghum vulgare Pers. are not resistant to seed deeplying of 20cm；Result with potted plant earthing experimental verification
It is not inconsistent；That is, under this condition of culture, judged result generation deviation can be caused.Additionally, there is also following defect: due to foam
Plastic plate is thinning, because mesocotyl is relatively soft, and meeting and the interlaced winding of seedling that closes in elongation process, impact growth, with
Difficulty during Shi Zengjia sampling；Cystosepiment is thickening, and the water of the splendid attire in Turnover Box to significantly reduce, and the oxygen being dissolved in water becomes
Few, seed is the most mouldy, affects emergence rate.
Finally, in addition it is also necessary to be only several specific embodiments of the present invention it is noted that listed above.Obviously, the present invention is not
It is limited to above example, it is also possible to have many deformation.Those of ordinary skill in the art directly can lead from present disclosure
The all deformation gone out or associate, are all considered as protection scope of the present invention.
Claims (6)
1. high flux screening is suitable for seed deeplying the method for sorghum variety, it is characterized in that comprising the following steps:
1), hydroponic device is set:
This hydroponic device is made up of container, plastic foamboard and gauze；
It is uniformly arranged the manhole that aperture is consistent on plastic foamboard, is fixed with gauze in the bottom of manhole；
Plastic foamboard is placed in equipped with in the container of water；
2), sowing and seedling are cultivated:
The seed of Sorghum vulgare Pers. to be measured being loaded manhole, carries out dark culturing under the constant temperature of 28 ± 0.5 DEG C, ambient humidity is
75% ± 2%, make sorghum seeds naturally sprout and emerge, incubation time is 6.5～7.5 days, obtains Sorghum Seedlings；
3), the mensuration of mesocotyl:
Step 2) gained Sorghum Seedlings measure mesocotyl length；
Under identical incubation time, the Sorghum vulgare Pers. to be measured of mesocotyl length is for relatively to be suitable for seed deeplying sorghum variety.
High flux screening the most according to claim 1 is suitable for seed deeplying the method for sorghum variety, it is characterized in that:
Described step 2) in, the Sorghum vulgare Pers. to be measured of each kind arranges 3 repetitions, 30 seeds of each repeat sowing；
Described step 3) in, from each repetition, randomly choose 10 strain Sorghum Seedlings carry out the mensuration of mesocotyl length；Take 30 plant height fine strains of millet
The meansigma methods of the mesocotyl of Seedling is as the mesocotyl length of this sorghum variety to be measured.
High flux screening the most according to claim 1 and 2 is suitable for seed deeplying the method for sorghum variety, it is characterized in that:
A diameter of 3.5 of manhole on described plastic foamboard～4.5cm；
The height of described manhole is 16～18mm.
High flux screening the most according to claim 3 is suitable for seed deeplying the method for sorghum variety, it is characterized in that: described gauze
Specification is 16 mesh.
5. it is suitable for seed deeplying the method for sorghum variety according to the arbitrary described high flux screening of Claims 1 to 4, it is characterized in that:
Described step 3) in,
As the mesocotyl length-depth of planting >=2cm of Sorghum vulgare Pers. to be measured, it is determined that this Sorghum vulgare Pers. to be measured belongs to the height of this depth of planting applicable
Fine strain of millet kind.
High flux screening the most according to claim 5 is suitable for seed deeplying the method for sorghum variety, it is characterized in that:
As the mesocotyl length >=12cm of Sorghum vulgare Pers. to be measured, it is determined that this Sorghum vulgare Pers. to be measured belongs to the Sorghum vulgare Pers. that applicable depth of planting is 10cm
Kind；
As the mesocotyl length >=22cm of Sorghum vulgare Pers. to be measured, it is determined that this Sorghum vulgare Pers. to be measured belongs to the Sorghum vulgare Pers. that applicable depth of planting is 20cm
Kind.
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| CN107549010A (en) * | 2017-09-28 | 2018-01-09 | 吉林省农业科学院 | A kind of method of the high sorghum variety of simple and quick screening emergence rate |
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| CN107549010A (en) * | 2017-09-28 | 2018-01-09 | 吉林省农业科学院 | A kind of method of the high sorghum variety of simple and quick screening emergence rate |
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