CN110286215A - 用于进行多路测定法的改进方法 - Google Patents
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Families Citing this family (54)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2904020C (en) * | 2013-03-11 | 2022-06-14 | Meso Scale Technologies, Llc. | Improved methods for conducting multiplexed assays |
| US9618510B2 (en) | 2013-03-13 | 2017-04-11 | Meso Scale Technologies, Llc. | Assay methods |
| CA2919833A1 (en) | 2013-07-30 | 2015-02-05 | President And Fellows Of Harvard College | Quantitative dna-based imaging and super-resolution imaging |
| KR102497054B1 (ko) | 2014-05-15 | 2023-02-06 | 메소 스케일 테크놀러지즈, 엘엘시 | 개선된 분석 방법 |
| CN116832890A (zh) | 2015-04-06 | 2023-10-03 | 中尺度技术有限责任公司 | 操作测定系统的方法及ecl免疫测定系统 |
| US10723988B2 (en) | 2015-04-22 | 2020-07-28 | Berkeley Lights, Inc. | Microfluidic cell culture |
| WO2017011560A1 (en) | 2015-07-15 | 2017-01-19 | Hycor Biomedical, Llc. | Customizable instrument |
| JP6858744B2 (ja) | 2015-08-07 | 2021-04-14 | プレジデント アンド フェローズ オブ ハーバード カレッジ | タンパク質−タンパク質相互作用の超分解能イメージング |
| US10799865B2 (en) | 2015-10-27 | 2020-10-13 | Berkeley Lights, Inc. | Microfluidic apparatus having an optimized electrowetting surface and related systems and methods |
| USD882818S1 (en) * | 2016-03-18 | 2020-04-28 | Meso Scale Technologies, Llc. | Reagent rack |
| USD1006251S1 (en) * | 2016-03-18 | 2023-11-28 | Meso Scale Technologies, Llc. | Rack and label |
| USD889685S1 (en) | 2016-03-18 | 2020-07-07 | Meso Scale Technologies, Llc. | Reagent rack cover |
| SG11201809539RA (en) | 2016-05-26 | 2018-12-28 | Berkeley Lights Inc | Covalently modified surfaces, kits, and methods of preparation and use |
| WO2018017949A1 (en) | 2016-07-22 | 2018-01-25 | Verily Life Sciences Llc | Quantitative massively parallel proteomics |
| WO2018025046A2 (en) * | 2016-08-05 | 2018-02-08 | Nn Scientific Limited | Device |
| US10618717B2 (en) * | 2017-07-31 | 2020-04-14 | Argos Technology, Inc. | Cryogenic storage box |
| WO2019069372A1 (ja) * | 2017-10-03 | 2019-04-11 | 株式会社ニコン | 検出対象の測定方法、捕捉プローブ固定化担体、検出キット、及び流体デバイス |
| EP4372383A3 (en) * | 2017-10-23 | 2024-11-06 | Nautilus Subsidiary, Inc. | Methods and systems for protein identification |
| EA202091646A1 (ru) * | 2018-01-05 | 2020-11-17 | Квошент Сюисс Са | Платформа на основе самособирающегося диагностического массива |
| EP3756009B1 (en) | 2018-02-23 | 2023-09-06 | Meso Scale Technologies, LLC | Methods of screening antigen-binding molecules by normalizing for the concentration of antigen-binding molecule |
| US11039856B2 (en) | 2018-05-10 | 2021-06-22 | Verily Life Sciences Llc | Surgical laparoscopic ports for implanting medical systems |
| WO2019222708A2 (en) | 2018-05-17 | 2019-11-21 | Meso Scale Technologies, Llc. | Methods for isolating surface marker displaying agents |
| US10936163B2 (en) | 2018-07-17 | 2021-03-02 | Methodical Mind, Llc. | Graphical user interface system |
| US20210382043A1 (en) | 2018-10-23 | 2021-12-09 | Meso Scale Technologies, Llc. | Methods for isolating surface marker displaying agents |
| US20220011301A1 (en) * | 2018-11-16 | 2022-01-13 | Roche Diagnostics Operations, Inc. | Streptavidin-coated solid phases with a member of a binding pair |
| WO2020104397A1 (en) | 2018-11-19 | 2020-05-28 | Bioaster | Methods and reagents for multiplex binding experiments |
| GB2579810B (en) * | 2018-12-14 | 2023-04-26 | Aeirtec Ltd | Assay Analysis |
| CA3132154A1 (en) * | 2019-03-01 | 2020-09-10 | Meso Scale Technologies, Llc. | Electrochemiluminescent labeled probes for use in immunoassay methods, methods using such and kits comprising same |
| SG11202111871WA (en) | 2019-05-03 | 2021-11-29 | Meso Scale Technologies Llc | Kits for detecting one or more target nucleic acid analytes in a sample and methods of making and using the same |
| AU2020380282A1 (en) | 2019-11-05 | 2022-05-26 | Meso Scale Technologies, Llc. | Methods and kits for quantitating radiation exposure |
| WO2021108666A2 (en) | 2019-11-26 | 2021-06-03 | Meso Scale Technologies, Llc. | Methods and kits for detecting autoimmune diseases |
| EP4143576A1 (en) | 2020-05-01 | 2023-03-08 | Meso Scale Technologies, LLC | Viral serology assays |
| WO2022020234A2 (en) * | 2020-07-20 | 2022-01-27 | Bio-Rad Laboratories, Inc. | Immunoassay for sars-cov-2 neutralizing antibodies and materials therefor |
| US11434525B2 (en) | 2020-08-06 | 2022-09-06 | Singular Genomics Systems, Inc. | Spatial sequencing |
| US11492662B2 (en) * | 2020-08-06 | 2022-11-08 | Singular Genomics Systems, Inc. | Methods for in situ transcriptomics and proteomics |
| AU2021338303A1 (en) | 2020-09-02 | 2023-04-20 | Meso Scale Technologies, Llc | Kits for detecting one or more target analytes in a sample and methods of making and using the same |
| WO2022051481A2 (en) | 2020-09-04 | 2022-03-10 | Meso Scale Technologies, Llc. | Methods for isolating central nervous system surface marker displaying agents |
| KR20230130683A (ko) | 2021-01-11 | 2023-09-12 | 메소 스케일 테크놀러지즈, 엘엘시 | 검정 시스템 교정 시스템 및 방법 |
| EP4302089A1 (en) | 2021-03-01 | 2024-01-10 | Meso Scale Technologies, LLC | System and method for conducting a multiplexed assay |
| WO2022231900A1 (en) | 2021-04-26 | 2022-11-03 | Meso Scale Technologies, Llc. | Methods for isolating and analyzing a target analyte encapsulated by a surface marker displaying agent |
| EP4334706A4 (en) | 2021-05-05 | 2025-08-06 | Singular Genomics Systems Inc | MULTIOMICS ANALYSIS DEVICE AND METHODS OF USE THEREOF |
| WO2022246215A1 (en) | 2021-05-21 | 2022-11-24 | Meso Scale Technologies, Llc. | Viral strain serology assays |
| CA3225576A1 (en) | 2021-06-28 | 2023-01-05 | Meso Scale Technologies, Llc. | Methods, compositions, and kits for assay signal amplification |
| CN118265967A (zh) | 2021-07-21 | 2024-06-28 | 方法思维有限责任公司 | 图形用户界面系统引导模块 |
| US20250362296A1 (en) | 2022-06-17 | 2025-11-27 | Meso Scale Technologies, Llc. | Viral strain serology assays |
| EP4591062A1 (en) | 2022-09-23 | 2025-07-30 | Meso Scale Technologies, LLC | Orthopoxvirus serology assays |
| EP4638775A1 (en) | 2022-12-20 | 2025-10-29 | Meso Scale Technologies, LLC | Assay methods and kits |
| KR20250141160A (ko) | 2023-01-09 | 2025-09-26 | 메쏘디컬 마인드, 엘엘씨. | 품질 관리를 위한 그래픽 사용자 인터페이스 시스템 |
| KR20250154387A (ko) | 2023-01-25 | 2025-10-28 | 메쏘디컬 마인드, 엘엘씨. | 실험실 조정 장치, 방법, 및 시스템 |
| WO2024242845A1 (en) | 2023-05-24 | 2024-11-28 | Meso Scale Technologies, Llc. | Methods for assessing colorectal cancer using extracellular vesicles |
| WO2024249961A1 (en) | 2023-06-01 | 2024-12-05 | Singular Genomics Systems, Inc. | Methods and probes for detecting polynucleotide sequences in cells and tissues |
| WO2025049305A1 (en) | 2023-08-25 | 2025-03-06 | Meso Scale Technologies, Llc. | Viral strain serology assays |
| WO2025188949A1 (en) | 2024-03-08 | 2025-09-12 | Meso Scale Technologies, Llc. | Methods of quantifying analytes |
| WO2025260043A1 (en) | 2024-06-14 | 2025-12-18 | Meso Scale Technologies, Llc. | Methods and kits for measuring three or more tau epitopes |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101208437A (zh) * | 2003-12-12 | 2008-06-25 | 圣路易斯大学 | 检测大分子和其它分析物的生物传感器 |
| CN101952719A (zh) * | 2007-07-27 | 2011-01-19 | 通信改革公司 | 检测测定法及其用途 |
Family Cites Families (121)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4161515A (en) | 1973-10-02 | 1979-07-17 | Syva Company | Double receptor fluorescent immunoassay |
| US4604263A (en) * | 1976-06-17 | 1986-08-05 | Analytical Products, Inc. | Cell for gas sample equilibrator |
| US4139346A (en) | 1977-11-28 | 1979-02-13 | Enzo Bio Chem Incorporated | Nucleic acid and protein binding paper |
| FI56750C (fi) | 1978-02-27 | 1980-03-10 | Reijo Vihko | Rekationskaerl foer engaongsbruk vid immunologiskt bestaemning |
| IL55816A (en) | 1978-10-30 | 1982-04-30 | Ames Yissum Ltd | Method for simultaneous immunoassay of several different antibodies and a kit therefor |
| US4378344A (en) | 1979-09-28 | 1983-03-29 | Ventrex Laboratories, Inc. | Method and apparatus for performing multiple, simultaneous in vitro diagnostic tests using a solid phase system |
| US4514508A (en) | 1982-07-06 | 1985-04-30 | Biond Inc. | Assaying for a multiplicity of antigens or antibodies with a detection compound |
| US4587044A (en) | 1983-09-01 | 1986-05-06 | The Johns Hopkins University | Linkage of proteins to nucleic acids |
| AU3844485A (en) | 1984-02-09 | 1985-08-15 | Enzo Biochem Inc. | Heterologous detection of cabeled dna |
| US4748111A (en) | 1984-03-12 | 1988-05-31 | Molecular Diagnostics, Inc. | Nucleic acid-protein conjugate used in immunoassay |
| CA1260372A (en) | 1984-04-27 | 1989-09-26 | Elazar Rabbani | Hybridization method for the detection of genetic materials |
| US5288609A (en) | 1984-04-27 | 1994-02-22 | Enzo Diagnostics, Inc. | Capture sandwich hybridization method and composition |
| US5238808A (en) | 1984-10-31 | 1993-08-24 | Igen, Inc. | Luminescent metal chelate labels and means for detection |
| US5310687A (en) | 1984-10-31 | 1994-05-10 | Igen, Inc. | Luminescent metal chelate labels and means for detection |
| JPH0823558B2 (ja) | 1984-11-27 | 1996-03-06 | オ−ジエニクス リミテツド | 検定装置 |
| JPH076984B2 (ja) | 1985-05-31 | 1995-01-30 | 株式会社日立製作所 | 複数項目分析方法 |
| US6165729A (en) | 1986-04-30 | 2000-12-26 | Hyperion Catalysis International, Inc. | Electrochemiluminescent reaction utilizing amine-derived reductant |
| US5591581A (en) | 1986-04-30 | 1997-01-07 | Igen, Inc. | Electrochemiluminescent rhenium moieties and methods for their use |
| US5147806A (en) | 1988-04-29 | 1992-09-15 | Igen, Inc. | Method and apparatus for conducting electrochemiluminescence measurements |
| US5849478A (en) | 1986-08-14 | 1998-12-15 | Cashman; Daniel P. | Blocked-polymerase polynucleotide immunoassay method and kit |
| US5750338A (en) | 1986-10-23 | 1998-05-12 | Amoco Corporation | Target and background capture methods with amplification for affinity assays |
| US4870003A (en) | 1987-06-15 | 1989-09-26 | Coulter Corporation | Simultaneous enzyme immunoassay for detecting antigen and/or antibody in humans |
| US5124246A (en) | 1987-10-15 | 1992-06-23 | Chiron Corporation | Nucleic acid multimers and amplified nucleic acid hybridization assays using same |
| US5525465A (en) | 1987-10-28 | 1996-06-11 | Howard Florey Institute Of Experimental Physiology And Medicine | Oligonucleotide-polyamide conjugates and methods of production and applications of the same |
| US5308754A (en) | 1988-03-21 | 1994-05-03 | Kankare Jouko J | Electrogenerated luminescence in solution |
| US5354655A (en) | 1988-03-29 | 1994-10-11 | Biocontrol Systems, Inc. | Method for determining the presence or concentration of a bound enzyme |
| US5093268A (en) | 1988-04-28 | 1992-03-03 | Igen, Inc. | Apparatus for conducting a plurality of simultaneous measurements of electrochemiluminescent phenomena |
| US5705402A (en) | 1988-11-03 | 1998-01-06 | Igen International, Inc. | Method and apparatus for magnetic microparticulate based luminescence assay including plurality of magnets |
| US6448091B1 (en) | 1988-11-03 | 2002-09-10 | Igen International, Inc. | Method and apparatus for improved luminescence assays using particle concentration chemiluminescence detection |
| CA2049036A1 (en) | 1989-03-21 | 1990-09-22 | Izak Bahar | Simultaneous dual analyte assay |
| US5391723A (en) | 1989-05-31 | 1995-02-21 | Neorx Corporation | Oligonucleotide conjugates |
| US6406844B1 (en) | 1989-06-07 | 2002-06-18 | Affymetrix, Inc. | Very large scale immobilized polymer synthesis |
| US5143854A (en) | 1989-06-07 | 1992-09-01 | Affymax Technologies N.V. | Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof |
| US5527681A (en) | 1989-06-07 | 1996-06-18 | Affymax Technologies N.V. | Immobilized molecular synthesis of systematically substituted compounds |
| CA2022518A1 (en) | 1989-08-04 | 1991-02-05 | Richard P. Watts | Heterogeneous binding assays |
| US5719063A (en) | 1989-08-25 | 1998-02-17 | Boehringer Mannheim Corporation | Multiplex immunoassay system |
| US5324457A (en) | 1989-10-02 | 1994-06-28 | Board Of Regents, The University Of Tx System | Devices and methods for generating electrogenerated chemiluminescence |
| US5629153A (en) | 1990-01-10 | 1997-05-13 | Chiron Corporation | Use of DNA-dependent RNA polymerase transcripts as reporter molecules for signal amplification in nucleic acid hybridization assays |
| CA2076349A1 (en) | 1990-02-22 | 1991-08-23 | Ashraf A. Ismail | Solid-phase interferometric immunoassay system |
| US5143852A (en) | 1990-09-14 | 1992-09-01 | Biosite Diagnostics, Inc. | Antibodies to ligand analogues and their utility in ligand-receptor assays |
| US5776672A (en) | 1990-09-28 | 1998-07-07 | Kabushiki Kaisha Toshiba | Gene detection method |
| US5726064A (en) | 1990-11-22 | 1998-03-10 | Applied Research Systems Ars Holding Nv | Method of assay having calibration within the assay |
| ZA92804B (en) | 1991-02-06 | 1992-12-30 | Igen Inc | Methods and apparatus for improved luminescence assays |
| JPH0534345A (ja) | 1991-02-19 | 1993-02-09 | Tdk Corp | 化学発光を利用する抗原・抗体の測定方法 |
| US5877028A (en) | 1991-05-29 | 1999-03-02 | Smithkline Diagnostics, Inc. | Immunochromatographic assay device |
| US5665539A (en) | 1991-07-12 | 1997-09-09 | The Regents Of The University Of California | Immuno-polymerase chain reaction system for antigen detection |
| AU2348092A (en) | 1991-07-16 | 1993-02-23 | Transmed Biotech Incorporated | Methods and compositions for simultaneous analysis of multiple analytes |
| EP0773227A1 (en) | 1991-09-18 | 1997-05-14 | Affymax Technologies N.V. | Diverse collections of oligomers in use to prepare drugs, diagnostic reagents, pesticides or herbicides |
| JP3097866B2 (ja) | 1991-10-15 | 2000-10-10 | マルティライト リミティド | 標識試薬を使用した結合検定法 |
| ZA929351B (en) | 1991-12-11 | 1993-06-04 | Igen Inc | Electrochemiluminescent label for DNA assays. |
| US5464746A (en) | 1991-12-17 | 1995-11-07 | Abbott Laboratories | Haptens, tracers, immunogens and antibodies for carbazole and dibenzofuran derivatives |
| US5573905A (en) | 1992-03-30 | 1996-11-12 | The Scripps Research Institute | Encoded combinatorial chemical libraries |
| US5518887A (en) | 1992-03-30 | 1996-05-21 | Abbott Laboratories | Immunoassays empolying generic anti-hapten antibodies and materials for use therein |
| US5795714A (en) | 1992-11-06 | 1998-08-18 | Trustees Of Boston University | Method for replicating an array of nucleic acid probes |
| US6087491A (en) * | 1993-01-08 | 2000-07-11 | Hybridon, Inc. | Extremely high purity oligonucleotides and methods of synthesizing them using dimer blocks |
| IT1270951B (it) | 1993-06-07 | 1997-05-26 | Boehringer Mannheim Italia | Metodo e dispositivo per il rilevamento simultano di neisseria gonorrhoeae, chlamydia trachomatis e mycoplasma |
| US5635602A (en) | 1993-08-13 | 1997-06-03 | The Regents Of The University Of California | Design and synthesis of bispecific DNA-antibody conjugates |
| US5789167A (en) * | 1993-09-10 | 1998-08-04 | Genevue, Inc. | Optical detection of position of oligonucleotides on large DNA molecules |
| US5415839A (en) | 1993-10-21 | 1995-05-16 | Abbott Laboratories | Apparatus and method for amplifying and detecting target nucleic acids |
| US7569341B2 (en) | 1994-01-31 | 2009-08-04 | Trustees Of Boston University | Nucleic acid directed immobilization arrays and methods of assembly |
| US5561043A (en) | 1994-01-31 | 1996-10-01 | Trustees Of Boston University | Self-assembling multimeric nucleic acid constructs |
| US5786141A (en) | 1994-08-26 | 1998-07-28 | Bard; Allen J. | Electrogenerated chemiluminescence labels for analysis and/or referencing |
| US5648213A (en) | 1994-08-30 | 1997-07-15 | Beckman Instruments, Inc. | Compositions and methods for use in detection of analytes |
| US5620850A (en) | 1994-09-26 | 1997-04-15 | President And Fellows Of Harvard College | Molecular recognition at surfaces derivatized with self-assembled monolayers |
| US5866434A (en) | 1994-12-08 | 1999-02-02 | Meso Scale Technology | Graphitic nanotubes in luminescence assays |
| US5643713A (en) | 1995-06-07 | 1997-07-01 | Liang; Pam | Electrochemiluminescent monitoring of compounds |
| US5641623A (en) | 1995-01-04 | 1997-06-24 | Martin; Mark T. | Electrochemiluminescence assay |
| US6207369B1 (en) | 1995-03-10 | 2001-03-27 | Meso Scale Technologies, Llc | Multi-array, multi-specific electrochemiluminescence testing |
| JPH11502617A (ja) | 1995-03-10 | 1999-03-02 | メソ スケール テクノロジーズ,エルエルシー | 多重アレイの多重特異的な電気化学発光試験 |
| US5722553A (en) * | 1995-03-31 | 1998-03-03 | Hovatter; Kenneth P. | Integral assembly of microcentrifuge strip tubes having independently tethered angularly related seal caps |
| US6319670B1 (en) | 1995-05-09 | 2001-11-20 | Meso Scale Technology Llp | Methods and apparatus for improved luminescence assays using microparticles |
| US5679519A (en) | 1995-05-09 | 1997-10-21 | Oprandy; John J. | Multi-label complex for enhanced sensitivity in electrochemiluminescence assay |
| US5589136A (en) | 1995-06-20 | 1996-12-31 | Regents Of The University Of California | Silicon-based sleeve devices for chemical reactions |
| US5723320A (en) | 1995-08-29 | 1998-03-03 | Dehlinger; Peter J. | Position-addressable polynucleotide arrays |
| WO1997014028A2 (en) | 1995-10-11 | 1997-04-17 | Luminex Corporation | Multiplexed analysis of clinical specimens apparatus and method |
| GB9606850D0 (en) | 1996-04-01 | 1996-06-05 | Univ Liverpool | An assay system and novel labelled compounds for use therwith |
| US6001310A (en) * | 1996-10-11 | 1999-12-14 | Shaffer; John V. | Pliable centrifuge tube array |
| CA2274315A1 (en) | 1996-12-06 | 1998-06-11 | Niels Pallisgaard | Detection of chromosomal abnormalities |
| FR2764381A1 (fr) | 1997-06-09 | 1998-12-11 | Univ De Neuchatel | Detecteur electrochimioluminescent |
| US6413783B1 (en) | 1997-09-18 | 2002-07-02 | Meso Scale Technologies, Llc | Assay sonication apparatus and methodology |
| US6200531B1 (en) | 1998-05-11 | 2001-03-13 | Igen International, Inc. | Apparatus for carrying out electrochemiluminescence test measurements |
| EP0962773A1 (en) | 1998-06-03 | 1999-12-08 | Mark Howard Jones | Electrochemical based assay processes instrument and labels |
| GB9815042D0 (en) | 1998-07-10 | 1998-09-09 | Imperial College | Detector |
| US6214552B1 (en) | 1998-09-17 | 2001-04-10 | Igen International, Inc. | Assays for measuring nucleic acid damaging activities |
| WO2000031536A2 (en) * | 1998-11-23 | 2000-06-02 | President And Fellows Of Harvard College | Detecting structural or synthetic information about chemical compounds |
| US6136268A (en) | 1999-08-17 | 2000-10-24 | Orion Diagnostica | Method for luminescence measurements |
| US20050287592A1 (en) * | 2000-08-29 | 2005-12-29 | Yeda Research And Development Co. Ltd. | Template-dependent nucleic acid polymerization using oligonucleotide triphosphates building blocks |
| US7070740B1 (en) | 2000-09-28 | 2006-07-04 | Beckman Coulter, Inc. | Method and apparatus for processing biomolecule arrays |
| US6601725B2 (en) * | 2001-05-15 | 2003-08-05 | 3088081 Canada, Inc. | Integral assembly of reagent tubes and seal caps |
| DK2423673T3 (da) | 2001-06-29 | 2020-09-07 | Meso Scale Technologies Llc | Indretning til måling af luminescens fra en flerbrønds-assayplade med en flerhed af brønde, fremgangsmåde til måling af luminescens med anvendelse af indretningen og system omfattende indretningen |
| US6942972B2 (en) | 2001-10-24 | 2005-09-13 | Beckman Coulter, Inc. | Efficient synthesis of protein-oligonucleotide conjugates |
| JP4657919B2 (ja) * | 2002-08-19 | 2011-03-23 | プレジデント アンド フェローズ オブ ハーバード カレッジ | 進化する新しい分子機能 |
| US20040049351A1 (en) * | 2002-08-28 | 2004-03-11 | Matson Robert S. | Immunosorbent assay in microarray format |
| US6913890B2 (en) * | 2002-12-18 | 2005-07-05 | Palo Alto Research Center Incorporated | Process for preparing albumin protein conjugated oligonucleotide probes |
| US7229763B2 (en) | 2003-04-07 | 2007-06-12 | Beckman Coulter, Inc. | Assay system using labeled oligonucleotides |
| US7195875B2 (en) | 2003-04-18 | 2007-03-27 | Beckman Coulter, Inc. | Oligonucleotide pairs for multiplexed binding assays |
| US20040258563A1 (en) * | 2003-06-23 | 2004-12-23 | Applera Corporation | Caps for sample wells and microcards for biological materials |
| JP2005164509A (ja) * | 2003-12-05 | 2005-06-23 | Hitachi High-Technologies Corp | 試薬容器 |
| EP1774033A4 (en) * | 2004-06-10 | 2008-04-23 | Perkinelmer Las Inc | MULTIPLEX ASSAYS FOR DETECTION OF ANALYTES |
| US7704730B2 (en) | 2004-10-14 | 2010-04-27 | Meso Scale Technologies, Llc | Multiplexed assay methods |
| US20060292559A1 (en) | 2005-06-23 | 2006-12-28 | Beckman Coulter, Inc. | Cell-based microarrays, and methods for their preparation and use |
| US7494776B2 (en) | 2005-07-07 | 2009-02-24 | Beckman Coulter, Inc. | Labeled complementary oligonucleotides to detect oligonucleotide-linked ligands |
| KR100927886B1 (ko) * | 2007-06-18 | 2009-11-23 | 한국생명공학연구원 | 단백질 g-올리고 뉴클레오타이드 결합체 |
| WO2009012185A1 (en) * | 2007-07-13 | 2009-01-22 | Handylab, Inc. | Polynucleotide capture materials, and methods of using same |
| JP2010533869A (ja) * | 2007-07-16 | 2010-10-28 | カリフォルニア インスティチュート オブ テクノロジー | 標的分子を検出するためのマイクロ流体装置、方法及びシステム |
| EP2088204A1 (en) * | 2008-02-05 | 2009-08-12 | Adnagen AG | Method and kit for diagnosing or controlling the treatment of ovarian cancer |
| JP2011527903A (ja) * | 2008-07-14 | 2011-11-10 | コーニンクレッカ フィリップス エレクトロニクス エヌ ヴィ | 細胞マイクロキャリアのアセンブリを供給するためのプロセス |
| CA2647953A1 (en) | 2008-12-29 | 2010-06-29 | Sqi Diagnostics Systems Inc. | Multiplex analyte detection |
| US20100261292A1 (en) | 2009-04-10 | 2010-10-14 | Meso Scale Technologies, Llc | Methods for Conducting Assays |
| US8480953B2 (en) * | 2009-05-20 | 2013-07-09 | Protedyne Corporation | System and method for vessel alignment |
| US20130184184A1 (en) * | 2010-11-22 | 2013-07-18 | The University Of Chicago | Methods and/or Use of Oligonucleotide Conjugates Having Varied Degrees of Labeling for Assays and Detections |
| US9182334B2 (en) * | 2012-02-24 | 2015-11-10 | Agilent Technologies, Inc. | Method of determining thermodynamic and kinetic parameters from measured off rates |
| JP5926990B2 (ja) * | 2012-03-16 | 2016-05-25 | シスメックス株式会社 | 検体処理装置 |
| US9180461B2 (en) * | 2012-10-22 | 2015-11-10 | Qiagen Gaithersburg, Inc. | Condensation-reducing incubation cover |
| USD703345S1 (en) * | 2012-10-29 | 2014-04-22 | Universal Bio Research Co., Ltd. | Plurality of reaction vessels with lids |
| EP4480582A1 (en) * | 2013-01-18 | 2024-12-25 | Biomeme, Inc. | Analytic device |
| CA2904020C (en) | 2013-03-11 | 2022-06-14 | Meso Scale Technologies, Llc. | Improved methods for conducting multiplexed assays |
| US9802196B2 (en) * | 2013-03-13 | 2017-10-31 | Alphagem Bio Inc. | Ergonomic numbered connector to hold tubes with improved cap |
| JP6387387B2 (ja) * | 2013-03-15 | 2018-09-05 | ベクトン・ディキンソン・アンド・カンパニーBecton, Dickinson And Company | プロセスチューブおよび搬送用トレイ |
| US8808650B1 (en) * | 2013-03-15 | 2014-08-19 | Shazi Iqbal | Foldable specimen rack with configurable specimen receiver |
| CA2919833A1 (en) * | 2013-07-30 | 2015-02-05 | President And Fellows Of Harvard College | Quantitative dna-based imaging and super-resolution imaging |
-
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- 2014-03-11 CA CA2904020A patent/CA2904020C/en active Active
- 2014-03-11 ES ES14778374T patent/ES2942031T3/es active Active
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101208437A (zh) * | 2003-12-12 | 2008-06-25 | 圣路易斯大学 | 检测大分子和其它分析物的生物传感器 |
| CN101952719A (zh) * | 2007-07-27 | 2011-01-19 | 通信改革公司 | 检测测定法及其用途 |
Non-Patent Citations (1)
| Title |
|---|
| CHRISTINA BOOZER ET AL.,: "DNA-Directed Protein Immobilization for Simultaneous Detection of Multiple Analytes by Surface Plasmon Resonance Biosensor", 《ANALYTICAL CHEMISTRY》 * |
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