CN110257412B - 一种新颖的细胞系筛选方法 - Google Patents

一种新颖的细胞系筛选方法 Download PDF

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Publication number
CN110257412B
CN110257412B CN201910335307.XA CN201910335307A CN110257412B CN 110257412 B CN110257412 B CN 110257412B CN 201910335307 A CN201910335307 A CN 201910335307A CN 110257412 B CN110257412 B CN 110257412B
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China
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reporter gene
dna
cell
protein
nucleic acid
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Expired - Fee Related
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CN201910335307.XA
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English (en)
Chinese (zh)
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CN110257412A (zh
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余波
詹姆斯·拉里克
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LARIX BIOSCIENCES LLC
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LARIX BIOSCIENCES LLC
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2887—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against CD20
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Food Science & Technology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201910335307.XA 2012-11-30 2013-11-27 一种新颖的细胞系筛选方法 Expired - Fee Related CN110257412B (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201910335307.XA CN110257412B (zh) 2012-11-30 2013-11-27 一种新颖的细胞系筛选方法

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201261732156P 2012-11-30 2012-11-30
US61/732,156 2012-11-30
CN201910335307.XA CN110257412B (zh) 2012-11-30 2013-11-27 一种新颖的细胞系筛选方法
CN201380062665.9A CN104870646B (zh) 2012-11-30 2013-11-27 一种新颖的细胞系筛选方法
PCT/US2013/072412 WO2014085711A1 (en) 2012-11-30 2013-11-27 A novel cell line screening method

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CN201380062665.9A Division CN104870646B (zh) 2012-11-30 2013-11-27 一种新颖的细胞系筛选方法

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CN110257412A CN110257412A (zh) 2019-09-20
CN110257412B true CN110257412B (zh) 2023-12-15

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US (2) US9910038B2 (https=)
EP (2) EP2925867B1 (https=)
JP (2) JP6442411B2 (https=)
CN (2) CN110257412B (https=)
WO (1) WO2014085711A1 (https=)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014085711A1 (en) * 2012-11-30 2014-06-05 Larix Biosciences Llc A novel cell line screening method
WO2016132122A1 (en) * 2015-02-17 2016-08-25 University Of Edinburgh Assay construct
CN119876218A (zh) * 2018-07-09 2025-04-25 杰诺维有限公司 用于在重组酶介导的核酸序列向工程化的接受细胞发生盒交换整合期间追踪和操纵细胞的细胞表面标签交换(cste)系统
CN112673109A (zh) * 2018-09-07 2021-04-16 免疫医疗有限责任公司 细胞选择方法
WO2020264253A1 (en) * 2019-06-26 2020-12-30 Genentech, Inc. Randomized configuration targeted integration of nucleic acids
WO2025176217A1 (en) * 2024-02-22 2025-08-28 The University Of Hong Kong Conditional splicing system for tight control of viral overlapping genes

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003070931A2 (de) * 2002-02-21 2003-08-28 Vision 7 Gmbh Verfahren zur ortsspezifischen dna-rekombination
WO2004007686A2 (en) * 2002-07-12 2004-01-22 Emory University Methods and transgenic mouse model for identifying and modulating factors involved in the production of reactive oxygen intermediates
CN101671653A (zh) * 2009-09-22 2010-03-17 浙江大学 一种hiv侵染细胞的筛选系统及其应用
CN102762728A (zh) * 2009-11-19 2012-10-31 株式会社免疫工学研究所 用于制备产生期望的多肽的抗体生产细胞的方法

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040038392A1 (en) * 2000-06-08 2004-02-26 Tatsurou Shibui Expression vector enabling screening of cells with high expression of recombinant protein, transformant with high expression of recombinant protein and utilization thereof
US6406863B1 (en) * 2000-06-23 2002-06-18 Genetastix Corporation High throughput generation and screening of fully human antibody repertoire in yeast
US20030059763A1 (en) * 2001-01-26 2003-03-27 Andrew Saxon Immunoglobulin class switch recombination
GB0118337D0 (en) 2001-07-27 2001-09-19 Lonza Biologics Plc Method for selecting antibody expressing cells
AU2003283995A1 (en) * 2002-09-30 2004-04-19 Protein Design Labs, Inc. Efficient generation of stable expression cell lines through the use of scorable homeostatic reporter genes
WO2005073375A1 (en) * 2004-01-30 2005-08-11 Maxygen Holdings Ltd. Regulated stop codon readthrough
KR20080073293A (ko) 2005-10-14 2008-08-08 메디뮨 엘엘씨 항체 라이브러리의 세포 디스플레이
WO2008070367A2 (en) 2006-11-01 2008-06-12 Facet Biotech Corporation Mammalian cell-based immunoglobulin display libraries
WO2008151219A1 (en) 2007-06-04 2008-12-11 Regeneron Pharmaceuticals, Inc. Enhanced expression and stability regions
US7947495B2 (en) 2007-11-01 2011-05-24 Abbott Biotherapeutics Corp. Immunoglobulin display vectors
DK2329020T3 (da) 2008-08-28 2013-06-10 Novartis Ag Celleoverfladepræsentation af polypeptidisoformer ved hjælp af stopcodon-overspringelse
JP2011182645A (ja) * 2010-03-04 2011-09-22 Kyushu Univ 動物細胞ディスプレイ法およびそれを利用した完全ヒトモノクローナル抗体の体外成熟法
WO2014085711A1 (en) * 2012-11-30 2014-06-05 Larix Biosciences Llc A novel cell line screening method

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003070931A2 (de) * 2002-02-21 2003-08-28 Vision 7 Gmbh Verfahren zur ortsspezifischen dna-rekombination
WO2004007686A2 (en) * 2002-07-12 2004-01-22 Emory University Methods and transgenic mouse model for identifying and modulating factors involved in the production of reactive oxygen intermediates
CN101671653A (zh) * 2009-09-22 2010-03-17 浙江大学 一种hiv侵染细胞的筛选系统及其应用
CN102762728A (zh) * 2009-11-19 2012-10-31 株式会社免疫工学研究所 用于制备产生期望的多肽的抗体生产细胞的方法

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
《Mouse model for lung tumorigenesis through Cre/lox controlled sporadic activation of the K-Ras oncogene》;Ralph Meuwissen等;《Oncogene》;20011019;第20卷;第6551-6558页 *
《phiC31 Integrase-Mediated Site-Specific Recombination in Barley》;Eszter Kapusi等;《PLOS ONE》;20120914;第7卷(第9期);第1-13页 *

Also Published As

Publication number Publication date
JP2018201520A (ja) 2018-12-27
CN104870646A (zh) 2015-08-26
US20180313834A1 (en) 2018-11-01
EP3382026A1 (en) 2018-10-03
WO2014085711A1 (en) 2014-06-05
CN104870646B (zh) 2019-05-28
CN110257412A (zh) 2019-09-20
JP2016506239A (ja) 2016-03-03
US20140155285A1 (en) 2014-06-05
EP2925867A1 (en) 2015-10-07
US10816548B2 (en) 2020-10-27
US9910038B2 (en) 2018-03-06
JP6785272B2 (ja) 2020-11-18
EP2925867B1 (en) 2018-06-27
EP3382026B1 (en) 2021-01-06
JP6442411B2 (ja) 2018-12-26

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Granted publication date: 20231215