JP6442411B2 - 新規な細胞株スクリーニング方法 - Google Patents

新規な細胞株スクリーニング方法 Download PDF

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Publication number
JP6442411B2
JP6442411B2 JP2015545470A JP2015545470A JP6442411B2 JP 6442411 B2 JP6442411 B2 JP 6442411B2 JP 2015545470 A JP2015545470 A JP 2015545470A JP 2015545470 A JP2015545470 A JP 2015545470A JP 6442411 B2 JP6442411 B2 JP 6442411B2
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cell
dna
sequence
cells
protein
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JP2015545470A
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Japanese (ja)
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JP2016506239A5 (https=
JP2016506239A (ja
Inventor
ユ，ボ
ラリック，ジェイムズ
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Larix Bioscience LLC
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Larix Bioscience LLC
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2887—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against CD20
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Food Science & Technology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2015545470A 2012-11-30 2013-11-27 新規な細胞株スクリーニング方法 Expired - Fee Related JP6442411B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261732156P 2012-11-30 2012-11-30
US61/732,156 2012-11-30
PCT/US2013/072412 WO2014085711A1 (en) 2012-11-30 2013-11-27 A novel cell line screening method

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2018167792A Division JP6785272B2 (ja) 2012-11-30 2018-09-07 新規な細胞株スクリーニング方法

Publications (3)

Publication Number Publication Date
JP2016506239A JP2016506239A (ja) 2016-03-03
JP2016506239A5 JP2016506239A5 (https=) 2016-12-28
JP6442411B2 true JP6442411B2 (ja) 2018-12-26

Family

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Family Applications (2)

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JP2015545470A Expired - Fee Related JP6442411B2 (ja) 2012-11-30 2013-11-27 新規な細胞株スクリーニング方法
JP2018167792A Expired - Fee Related JP6785272B2 (ja) 2012-11-30 2018-09-07 新規な細胞株スクリーニング方法

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JP2018167792A Expired - Fee Related JP6785272B2 (ja) 2012-11-30 2018-09-07 新規な細胞株スクリーニング方法

Country Status (5)

Country Link
US (2) US9910038B2 (https=)
EP (2) EP2925867B1 (https=)
JP (2) JP6442411B2 (https=)
CN (2) CN110257412B (https=)
WO (1) WO2014085711A1 (https=)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014085711A1 (en) * 2012-11-30 2014-06-05 Larix Biosciences Llc A novel cell line screening method
WO2016132122A1 (en) * 2015-02-17 2016-08-25 University Of Edinburgh Assay construct
CN119876218A (zh) * 2018-07-09 2025-04-25 杰诺维有限公司 用于在重组酶介导的核酸序列向工程化的接受细胞发生盒交换整合期间追踪和操纵细胞的细胞表面标签交换(cste)系统
CN112673109A (zh) * 2018-09-07 2021-04-16 免疫医疗有限责任公司 细胞选择方法
WO2020264253A1 (en) * 2019-06-26 2020-12-30 Genentech, Inc. Randomized configuration targeted integration of nucleic acids
WO2025176217A1 (en) * 2024-02-22 2025-08-28 The University Of Hong Kong Conditional splicing system for tight control of viral overlapping genes

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040038392A1 (en) * 2000-06-08 2004-02-26 Tatsurou Shibui Expression vector enabling screening of cells with high expression of recombinant protein, transformant with high expression of recombinant protein and utilization thereof
US6406863B1 (en) * 2000-06-23 2002-06-18 Genetastix Corporation High throughput generation and screening of fully human antibody repertoire in yeast
US20030059763A1 (en) * 2001-01-26 2003-03-27 Andrew Saxon Immunoglobulin class switch recombination
GB0118337D0 (en) 2001-07-27 2001-09-19 Lonza Biologics Plc Method for selecting antibody expressing cells
WO2003070931A2 (de) * 2002-02-21 2003-08-28 Vision 7 Gmbh Verfahren zur ortsspezifischen dna-rekombination
WO2004007686A2 (en) * 2002-07-12 2004-01-22 Emory University Methods and transgenic mouse model for identifying and modulating factors involved in the production of reactive oxygen intermediates
AU2003283995A1 (en) * 2002-09-30 2004-04-19 Protein Design Labs, Inc. Efficient generation of stable expression cell lines through the use of scorable homeostatic reporter genes
WO2005073375A1 (en) * 2004-01-30 2005-08-11 Maxygen Holdings Ltd. Regulated stop codon readthrough
KR20080073293A (ko) 2005-10-14 2008-08-08 메디뮨 엘엘씨 항체 라이브러리의 세포 디스플레이
WO2008070367A2 (en) 2006-11-01 2008-06-12 Facet Biotech Corporation Mammalian cell-based immunoglobulin display libraries
WO2008151219A1 (en) 2007-06-04 2008-12-11 Regeneron Pharmaceuticals, Inc. Enhanced expression and stability regions
US7947495B2 (en) 2007-11-01 2011-05-24 Abbott Biotherapeutics Corp. Immunoglobulin display vectors
DK2329020T3 (da) 2008-08-28 2013-06-10 Novartis Ag Celleoverfladepræsentation af polypeptidisoformer ved hjælp af stopcodon-overspringelse
CN101671653B (zh) * 2009-09-22 2012-05-23 浙江大学 一种hiv侵染细胞的筛选系统及其应用
WO2011061937A1 (en) * 2009-11-19 2011-05-26 Immuno Tec Laboratory Co., Ltd. Methods for producing antibody-producing cells that produce desired polypeptides
JP2011182645A (ja) * 2010-03-04 2011-09-22 Kyushu Univ 動物細胞ディスプレイ法およびそれを利用した完全ヒトモノクローナル抗体の体外成熟法
WO2014085711A1 (en) * 2012-11-30 2014-06-05 Larix Biosciences Llc A novel cell line screening method

Also Published As

Publication number Publication date
JP2018201520A (ja) 2018-12-27
CN104870646A (zh) 2015-08-26
US20180313834A1 (en) 2018-11-01
EP3382026A1 (en) 2018-10-03
WO2014085711A1 (en) 2014-06-05
CN104870646B (zh) 2019-05-28
CN110257412A (zh) 2019-09-20
JP2016506239A (ja) 2016-03-03
CN110257412B (zh) 2023-12-15
US20140155285A1 (en) 2014-06-05
EP2925867A1 (en) 2015-10-07
US10816548B2 (en) 2020-10-27
US9910038B2 (en) 2018-03-06
JP6785272B2 (ja) 2020-11-18
EP2925867B1 (en) 2018-06-27
EP3382026B1 (en) 2021-01-06

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