CN104870646B - 一种新颖的细胞系筛选方法 - Google Patents

一种新颖的细胞系筛选方法 Download PDF

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Publication number
CN104870646B
CN104870646B CN201380062665.9A CN201380062665A CN104870646B CN 104870646 B CN104870646 B CN 104870646B CN 201380062665 A CN201380062665 A CN 201380062665A CN 104870646 B CN104870646 B CN 104870646B
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cell
dna
antibody
sequence
expression
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Chinese (zh)
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CN104870646A (zh
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余波
詹姆斯·拉里克
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Shanghai Tongwen Biotechnology Co ltd
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LARIX BIOSCIENCES LLC
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2887—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against CD20
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Hematology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Food Science & Technology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201380062665.9A 2012-11-30 2013-11-27 一种新颖的细胞系筛选方法 Active CN104870646B (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201910335307.XA CN110257412B (zh) 2012-11-30 2013-11-27 一种新颖的细胞系筛选方法

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261732156P 2012-11-30 2012-11-30
US61/732,156 2012-11-30
PCT/US2013/072412 WO2014085711A1 (en) 2012-11-30 2013-11-27 A novel cell line screening method

Related Child Applications (1)

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CN201910335307.XA Division CN110257412B (zh) 2012-11-30 2013-11-27 一种新颖的细胞系筛选方法

Publications (2)

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CN104870646A CN104870646A (zh) 2015-08-26
CN104870646B true CN104870646B (zh) 2019-05-28

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CN201380062665.9A Active CN104870646B (zh) 2012-11-30 2013-11-27 一种新颖的细胞系筛选方法
CN201910335307.XA Expired - Fee Related CN110257412B (zh) 2012-11-30 2013-11-27 一种新颖的细胞系筛选方法

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Country Status (5)

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US (2) US9910038B2 (https=)
EP (2) EP2925867B1 (https=)
JP (2) JP6442411B2 (https=)
CN (2) CN104870646B (https=)
WO (1) WO2014085711A1 (https=)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2925867B1 (en) * 2012-11-30 2018-06-27 Larix Biosciences LLC A novel cell line screening method
WO2016132122A1 (en) * 2015-02-17 2016-08-25 University Of Edinburgh Assay construct
KR20210030414A (ko) * 2018-07-09 2021-03-17 제노비에 에이비 조작된 수용체 세포로의 핵산 서열의 재조합효소 매개 카세트 교환 통합 동안 세포의 추적 및 조작을 위한 세포 표면 태그 교환(cste) 시스템
CN112673109A (zh) * 2018-09-07 2021-04-16 免疫医疗有限责任公司 细胞选择方法
AU2020308002A1 (en) * 2019-06-26 2022-01-20 Genentech, Inc. Randomized configuration targeted integration of nucleic acids
WO2025176217A1 (en) * 2024-02-22 2025-08-28 The University Of Hong Kong Conditional splicing system for tight control of viral overlapping genes

Citations (2)

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WO2004007686A2 (en) * 2002-07-12 2004-01-22 Emory University Methods and transgenic mouse model for identifying and modulating factors involved in the production of reactive oxygen intermediates
CN102762728A (zh) * 2009-11-19 2012-10-31 株式会社免疫工学研究所 用于制备产生期望的多肽的抗体生产细胞的方法

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EP1293564A4 (en) * 2000-06-08 2005-08-17 Mitsubishi Pharma Corp EXPRESSION VECTOR FOR SCREENING CELLS HIGH EXPRESSION OF RECOMBINANT PROTEINS, TRANSFORMING HIGH EXPRESSION OF A RECOMBINANT PROTEIN AND USE THEREOF
US6406863B1 (en) 2000-06-23 2002-06-18 Genetastix Corporation High throughput generation and screening of fully human antibody repertoire in yeast
US20030059763A1 (en) * 2001-01-26 2003-03-27 Andrew Saxon Immunoglobulin class switch recombination
GB0118337D0 (en) 2001-07-27 2001-09-19 Lonza Biologics Plc Method for selecting antibody expressing cells
WO2003070931A2 (de) 2002-02-21 2003-08-28 Vision 7 Gmbh Verfahren zur ortsspezifischen dna-rekombination
US20040115814A1 (en) * 2002-09-30 2004-06-17 Protein Design Labs, Inc. Efficient generation of stable expression cell lines through the use of scorable homeostatic reporter genes
DK1716233T3 (da) * 2004-01-30 2009-10-26 Maxygen Holdings Ltd Reguleret stopkodongennemlæsning
KR20080073293A (ko) 2005-10-14 2008-08-08 메디뮨 엘엘씨 항체 라이브러리의 세포 디스플레이
WO2008070367A2 (en) 2006-11-01 2008-06-12 Facet Biotech Corporation Mammalian cell-based immunoglobulin display libraries
EP2150617B1 (en) 2007-06-04 2014-10-22 Regeneron Pharmaceuticals, Inc. Enhanced expression and stability regions
US7947495B2 (en) 2007-11-01 2011-05-24 Abbott Biotherapeutics Corp. Immunoglobulin display vectors
AU2009286956B2 (en) 2008-08-28 2012-12-06 Novartis Ag Cell surface display of polypeptide isoforms by stop codon readthrough
CN101671653B (zh) * 2009-09-22 2012-05-23 浙江大学 一种hiv侵染细胞的筛选系统及其应用
JP2011182645A (ja) * 2010-03-04 2011-09-22 Kyushu Univ 動物細胞ディスプレイ法およびそれを利用した完全ヒトモノクローナル抗体の体外成熟法
EP2925867B1 (en) * 2012-11-30 2018-06-27 Larix Biosciences LLC A novel cell line screening method

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004007686A2 (en) * 2002-07-12 2004-01-22 Emory University Methods and transgenic mouse model for identifying and modulating factors involved in the production of reactive oxygen intermediates
CN102762728A (zh) * 2009-11-19 2012-10-31 株式会社免疫工学研究所 用于制备产生期望的多肽的抗体生产细胞的方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Construction and development of a mammalian cell-based full-length antibody display library for targeting hepatocellular carcinoma;Feng Li et al;《Appl Microbiol Biotechnol》;20120707;第96卷(第5期);1233-1241 *

Also Published As

Publication number Publication date
CN104870646A (zh) 2015-08-26
JP2018201520A (ja) 2018-12-27
JP6785272B2 (ja) 2020-11-18
US20140155285A1 (en) 2014-06-05
US10816548B2 (en) 2020-10-27
US9910038B2 (en) 2018-03-06
EP2925867B1 (en) 2018-06-27
JP2016506239A (ja) 2016-03-03
CN110257412B (zh) 2023-12-15
JP6442411B2 (ja) 2018-12-26
EP3382026A1 (en) 2018-10-03
US20180313834A1 (en) 2018-11-01
EP2925867A1 (en) 2015-10-07
EP3382026B1 (en) 2021-01-06
CN110257412A (zh) 2019-09-20
WO2014085711A1 (en) 2014-06-05

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