CN110235858A - A method of Storage tobacco cacac moth is killed so that cacac moth is host's feeding transport microbracon hebetor bee pupa - Google Patents

A method of Storage tobacco cacac moth is killed so that cacac moth is host's feeding transport microbracon hebetor bee pupa Download PDF

Info

Publication number
CN110235858A
CN110235858A CN201910489263.6A CN201910489263A CN110235858A CN 110235858 A CN110235858 A CN 110235858A CN 201910489263 A CN201910489263 A CN 201910489263A CN 110235858 A CN110235858 A CN 110235858A
Authority
CN
China
Prior art keywords
box
cacac moth
bee
moth
cacac
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201910489263.6A
Other languages
Chinese (zh)
Inventor
金鑫
杨茂发
刁朝强
廖勇
欧后丁
杨洪
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Guizhou University
Guiyang Office of Guizhou Tobacco Corp
Original Assignee
Guizhou University
Guiyang Office of Guizhou Tobacco Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Guizhou University, Guiyang Office of Guizhou Tobacco Corp filed Critical Guizhou University
Priority to CN201910489263.6A priority Critical patent/CN110235858A/en
Publication of CN110235858A publication Critical patent/CN110235858A/en
Pending legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
    • A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/20—Animal feeding-stuffs from material of animal origin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/105—Aliphatic or alicyclic compounds
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/111—Aromatic compounds
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/163—Sugars; Polysaccharides
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/195—Antibiotics
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B1/00—Preparation of tobacco on the plantation
    • A24B1/02—Arrangements in barns for preparatory treatment of the tobacco, e.g. with devices for drying

Abstract

It is that host raises the method for transporting microbracon hebetor bee pupa to kill Storage tobacco cacac moth that the invention discloses a kind of with cacac moth, belongs to snout moth class pest storage Prevention Technique field；It the following steps are included: S1, configure cacac moth feed；S2, growing environment is provided for cacac moth and periodically cacac moth ovum is collected；The feed that S1 is deployed is added into the first raising box in S3, transfer tobacco powder snout moth's larva ovum thereto；S4, will hatch later cacac moth larva be transferred in the second raising box and to second raising box in be put into 1 pairs of generation microbracon hebetor into bee；S5, it is collected after 2 generation microbracon hebetors become bee pupa from ovum, larva from the second raising box, and transfers them in transport case progress entrucking processing；S6, box to be transported carry out putting bee after being transferred to tobacco warehouse, carry out parasitism to the cacac moth of Storage tobacco and kill；The present invention is implemented simply, conducive to the insect pest for solving cacac moth on Storage tobacco.

Description

One kind killing storage cigarette so that cacac moth is host's feeding transport microbracon hebetor bee pupa The method of leaf cacac moth
Technical field
The present invention relates to snout moth class pest storage Prevention Technique fields, and in particular to one kind is host's feeding with cacac moth The method for transporting microbracon hebetor bee pupa to kill Storage tobacco cacac moth.
Background technique
Cacac moth Ephestia elutella (H ü bner), is subordinate to Lepidoptera Lepidoptera, Pyralidae Pyralidae also known as tobacco powder sp, tobacco snout moth etc. are distributed widely in countries in the world and China except Heilungkiang, Jilin, new Various regions (Luo Meihao and Li Zhengyue, 2010) other than boundary, Tibet (not in detail), Yunnan and Guizhou occur it is heavier (high family closes etc., 1999), each tabacco barn in Guizhou is distributed and occurs (Chen Xun and Gao Nianzhao, 1996).Larva feeding habits are wide, and not only cause harm Storage period Tobacco leaf, can also cause harm cocoa, wheat etc., it is broken incomplete to make stored tobacco leaf, or even only stay vein, influence cigarette chopping technique and want It asks, the frass that generates during causing harm, worm corpse (Proteinaceous foreign matter) can lead to quality of tobacco and decline 1~2 grade (Gong Xinwen Deng 1995)；According to research reports, pine tobacco factory, Guizhou Province in 1986 is up to 57% (Du Yan because of the cacac moth tobacco leaf loss late that causes harm It is beautiful etc., 2000), the tobacco leaf for causing China to lose about 31,150,000 kg every year of causing harm jointly with other storage pests.Existing research table The parasitism that bright microbracon hebetor can effectively carry out cacac moth is killed, to play the effect of biological control；Compared to physics Or chemical means handle cacac moth, biological control have pollute it is small, it is rapid-action, and degree is killed to cacac moth in warehouse High advantage, but it is directed to how big batch kills cacac moth using microbracon hebetor at present, domestic research Still in space state, also lack corresponding device or means handle from feeding host put bee to warehouse during occur one Series of problems.
Summary of the invention
The technical problems to be solved by the present invention are: providing a kind of raise with cacac moth for host transports microbracon hebetor bee Pupa is lacked for batch using microbracon hebetor with solving the current country in warehouse come the method for killing Storage tobacco cacac moth The problem of effective ways that cacac moth is killed.
To solve the above problems, the present invention provides the following technical scheme that
A method of Storage tobacco cacac moth is killed so that cacac moth is host's feeding transport microbracon hebetor bee pupa； The following steps are included:
S1, preparation raise the man-made feeds of cacac moth larva: the man-made feeds include the component of following parts by weight: Dry corn sand 27-33%, dry crushing wheat 36.3-73.3%, yeast powder 5.5-8.5%, full fat soybean powder 1.2-2.8%, oatmeal 0-7%, Buddhist nun's platinum methyl esters 0.15-0.35%, sorbic acid 0.10-0.4%, Moroxydine Hydrochloride piece 0.1-0.3%, glycerol 6-8%, Honey 5-9% and water 2.5-3.5%；It is prepared by being stirred after being weighed above-mentioned material；
S2, the cacac moth adult group for the 1-3d that sprouts wings is transferred in oviposition box, the environment temperature for box of laying eggs is 27-29 DEG C, relative humidity 70-80% and photoperiod 16L；And the bee syrup that addition concentration is 20-30% into oviposition box daily, and with Collection of the period of 1-3d to ovum is carried out in oviposition box；
S3, the man-made feeds prepared in S1 are placed in the first raising box, then the ovum collected in S2 is uniformly sprinkling upon first It raises in box, and the first raising box is placed in environmental condition environment identical with oviposition box；
S4, after cacac moth egg hatching is at larva by the cacac moth larva of certain amount from first raising box be transferred to Second raising box, and 1 pairs of generation microbracon hebetor is introduced into the second raising box at bee；It is added daily into the second raising box dense Degree is the bee syrup of 10-70%；And the second raising box is placed in environmental condition environment identical with oviposition box；Deng 1 generation wheat Moth cocoon bee carries out cacac moth larva 2 generation microbracon hebetors can be obtained after parasitic oviposition；
S5, after 2 generation microbracon hebetor ovum in S4 via a length of larva of oviparity and after tying pupa by bee pupa from the second raising box It is transferred in transport case and carries out entrucking, and guarantee that the temperature in transportation environment locating for transport case is 12-18 DEG C；
S6, microbracon hebetor in transport case bee pupa sprout wings before transport case is transported in the warehouse for having tobacco leaf to storage, And the bee pupa of the microbracon hebetor in transport case is transferred in bee-escape；It can be by microbracon hebetor at bee after microbracon hebetor sprouts wings Parasitism is carried out to cacac moth on Storage tobacco to kill.
Preferably, in the step S2 number of cacac moth larva and pairs of microbracon hebetor logarithm ratio 10:1.
Preferably, it is 10cm that the size of the second raising box, which is basal diameter, the cylinder box body structure of a height of 8cm.
Preferably, the second raising box used in the step S4 includes tank body；Tank body is both ends open inner hollow Column structure；And it is removably installed top cover and bottom cover respectively at the both ends of tank body；Be provided on top cover ventilation through-hole and Location hole, and separation net is covered on ventilation through-hole；Tank inner chamber is inserted by location hole in one end of nutrition liquid pipe；In nutrition The lower bottom part of liquid pipe is fixed with cotton mass；Bottom cover includes the mounting disc being connected with tank body；In mounting disc with the inner cavity phase of tank body Pair end face be provided with connector；Catch tray is installed on the other end of the connector；The size of catch tray and the inner wall of tank body Size matches.
Further, external thread section is had at the both ends of tank body, is provided with and the external screw thread on top cover and bottom cover The internal thread segment that section matches；Mounting disc on bottom cover is the integral structure with ring wall and disk；Internal thread segment is arranged in institute It states on ring wall；The rod-like structure at the middle part of the disk is arranged in connector position；Mounting groove is provided on the side wall of catch tray；And Sealing ring is installed on the mounting groove；Once mild-natured gasket is removably installed in the upper surface of catch tray.
Preferably, the transport case used in the step S5；External packing box including inner hollow, in external packing box It has been removably installed active box, and has had the first air inlet and first on two opposite side end faces of external packing box Venthole；First air inlet is connected by pipeline with the outlet side that external cold air pumps；Be provided on the side end face of active box with Opposite the second air inlet and the second venthole of first air inlet and the first outlet hole site is in the intracorporal medium position of active box Partition is installed；And active box is partitioned into several cells by the partition；And the inner wall of each cell is both provided with Locating piece；Storage bee tank is detachably disposed in active box by the locating piece.
Further, the first air inlet is arranged at the adjacent position in two side end faces of external packing box；On active box The second air inlet be the different aperture of multiple sizes for being distributed on its side end face；And from close to far from the first air inlet Position, the size of the aperture are in the trend being gradually increased；First venthole and the second venthole be all disposed within far from first into The side of stomata.
Further, locating slot is provided on the inner bottom surface of external packing box；The bottom surface of active box be provided with The inserted block that the locating slot matches；It is soft that buffering is also equipped between bottom in external packing box and active box outer bottom Pad；Storage bee tank is cylindrical structure；The column block structure that locating piece is placed in cell in four cruciform symmetries；And locating piece The side to connect with storage bee tank is arcuate structure.
Preferably, the oviposition box used in the step S2 includes bottle body and the bottle cap that is arranged on bottle body, The upper surface of the bottle cap offers air hole, the first gauze for sealing is equipped in the air hole, in the bottle body Be provided at below the bottleneck into insect population, it is described enter insect population at sealed with tampon, offer circular hole in the bottom of the bottle body, It is equipped with the second gauze for sealing in the circular hole, collection ovum disk is provided with below the bottle body.
The invention has the advantages that:
The beneficial effects of the present invention are embodied in the following aspects: 1, by configuring the feed of cacac moth and providing its life Environmental condition needed for long oviposition is efficiently solved to be difficult to find during cultivating microbracon hebetor suitably to lay eggs and be posted It is main, thus the problem of can not cultivating in batches；2, reliable ring is provided for growth courses such as the 1 normal mate and oviposits of generation microbracon hebetor Border condition improves the cacac moth larva as host by parasitic rate, to increase the number for cultivating 2 generation microbracon hebetors；3, it provides The method of the transfer of microbracon hebetor bee pupa batch, packaging and transport, can be improved the life cycle of microbracon hebetor, to make difference The client in region can obtain using.On the other hand, the present invention has also separately designed the production laid eggs convenient for cacac moth Ovum box raises the raising box and transport case of microbracon hebetor, inconvenient to solve the collection faced in the specific implementation process, deposits at bee The lower problem of motility rate.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the second raising box in the present invention；
Fig. 2 is the structural schematic diagram of transport case in the present invention；
Fig. 3 is the A direction view of Fig. 2；
Fig. 4 is the structural schematic diagram of oviposition box in the present invention；
Description of symbols: 1, bottle body, 2, bottle cap, 3, air hole, the 4, first gauze, 5, enter insect population, 6, tampon；7, Two gauzes, 8, collection ovum disk；
1A, tank body, 2A, top cover, 3A, bottom cover, 4A, external thread section, 5A, internal thread segment, 6A, separation net, 7A, nutrition liquid pipe A, 8A, cotton mass, 11A, auxiliary ventilation through-hole, 21A, ventilation through-hole, 22A, location hole, 31A, mounting disc, 32A, connector, 33A, catch tray, 34A, sealing ring, 331A, mounting groove, 332A, disposable flat shim；
1B, external packing box, 2B, active box, 3B, cold air pump, 4B, partition, 5B, locating piece, 6B, storage bee tank, 7B, buffering Cushion, 11B, the first air inlet, 12B, the first venthole, 13B, locating slot, 21B, the second air inlet, 22B, the second outlet Hole, 23B, cell, 24B, inserted block.
Specific embodiment
With reference to the accompanying drawing and the present invention is described further in specific embodiment:
Embodiment:
Referring to Fig.1, the present embodiment provides one kind kills storehouse so that cacac moth is host's feeding transport microbracon hebetor bee pupa The method for storing up tobacco leaf cacac moth, comprising the following steps:
S1, preparation raise the man-made feeds of cacac moth larva: the man-made feeds include the component of following parts by weight: Dry corn sand 28%, dry crushing wheat 42.3%, yeast powder 7%, full fat soybean powder 2.5%, oatmeal 3%, Buddhist nun's platinum methyl esters 0.25%, sorbic acid 0.25%, Moroxydine Hydrochloride piece 0.2%, glycerol 7%, honey 6.5% and water 3%；Its prepare pass through by Above-mentioned material is stirred after being weighed；
S2, the cacac moth adult group for the 1-3d that sprouts wings being transferred in oviposition box, the environment temperature for box of laying eggs is 28 DEG C, Relative humidity 75% and photoperiod 16L；And the bee syrup that addition concentration is 25% into oviposition box daily, and with the period of 1-3d Collection to ovum is carried out in oviposition box；
S3, the man-made feeds prepared in S1 are placed in the first raising box, then the ovum collected in S2 is uniformly sprinkling upon first It raises in box, and the first raising box is placed in environmental condition environment identical with oviposition box；
S4, after cacac moth egg hatching is at larva by the cacac moth larva of certain amount from first raising box be transferred to Second raising box, and 1 pairs of generation microbracon hebetor is introduced into the second raising box at bee；It is added daily into the second raising box dense Degree is the bee syrup of 10-70%；And the second raising box is placed in environmental condition environment identical with oviposition box；Deng 1 generation wheat Moth cocoon bee carries out cacac moth larva 2 generation microbracon hebetors can be obtained after parasitic oviposition；
S5, after 2 generation microbracon hebetor ovum in S4 via a length of larva of oviparity and after tying pupa by bee pupa from the second raising box It is transferred in transport case and carries out entrucking, and guarantee that the temperature in transportation environment locating for transport case is 12-18 DEG C；
S6, microbracon hebetor in transport case bee pupa sprout wings before transport case is transported in the warehouse for having tobacco leaf to storage, And the bee pupa of the microbracon hebetor in transport case is transferred in bee-escape；It can be by microbracon hebetor at bee after microbracon hebetor sprouts wings Parasitism is carried out to cacac moth on Storage tobacco to kill.
The logarithm ratio 10:1 of the number of cacac moth larva and pairs of microbracon hebetor in the step S2.
It is 10cm that the size of the second raising box, which is basal diameter, the cylinder box body structure of a height of 8cm.
The second raising box used in the step S4 includes tank body 1A；Tank body 1A is the column of both ends open inner hollow Shape structure；And it is removably installed top cover 2A and bottom cover 3A respectively at the both ends of tank body 1A；It is logical that ventilation is provided on top cover 2A Hole 21A and location hole 22A, and separation net 6A is covered on ventilation through-hole 21A；One end of nutrition liquid pipe 7A passes through location hole 22A is inserted into the inner cavity tank body 1A；Cotton mass 8A is fixed in the lower bottom part of nutrition liquid pipe 7A；Bottom cover 3A includes being connected with tank body 1A Mounting disc 31A；The end face opposite with the inner cavity of tank body 1A is provided with connector 32A on mounting disc 31A；In connector 32A The other end on catch tray 33A is installed；The size of catch tray 33A and the inner wall size of tank body 1A match.
External thread section 4A is had at the both ends of tank body 1A, is provided with and the external thread section on top cover 2A and bottom cover 3A The internal thread segment 5A to match；Mounting disc 31A on bottom cover 3A is the integral structure with ring wall and disk；Internal thread segment 5A is set It sets on the ring wall；Connector 32A is the rod-like structure that the middle part of the disk is arranged in；It is opened on the side wall of catch tray 33A There is mounting groove 331A；And sealing ring 34A is installed on the mounting groove 331A；The upper surface of catch tray 33A removably Disposable flat shim 332A is installed.Cleaning arrangement is carried out after dismantling for convenience to device, and to microbracon hebetor bee pupa It collects, so devising disposable flat shim 332A, in the present embodiment, disposable flat shim 332A is thin using rigid plastics Piece, and being mounted on catch tray 33A by clamping, when cleaning can throw away after disposable flat shim 332A to catch tray 33A into Row cleaning, while being recycled convenient for device.
During device raises microbracon hebetor and collects bee pupa in using the present embodiment, first disposable Flat shim 332A is placed on catch tray 33A, will be placed with nutrition liquid pipe 7A along location hole 22A, and in nutrition liquid pipe 7A The installation of the side end inside tank body 1A cotton mass is protruded into, and by bottom cover 3A and tank body 1A by being threadably mounted at together, by cigarette Grass meal snout moth's larva larva is put into tank body 1A, is introducing pairs of 1A thereto for microbracon hebetor adult, is having nutrition liquid pipe for above-mentioned The bottom cover 2A of 7A is installed at the top of tank body 1A and is fixed；Device, which is placed in proper environment condition, makes it be normally carried out growth Activity, by being put into 20% bee syrup inside nutrition liquid pipe 7A timing phase tank body 1A to supplement the nutrients, in 1 generation microbracon hebetor by 2 It is produced in cacac moth larva for microbracon hebetor ovum parasitism, and 2 generation microbracon hebetors by ovum, larva and dismantle bottom cover after cocooing 3A takes out disposable flat shim 332A, using bee pupa under brush brush to complete to collect.
The transport case used in the step S5；External packing box 1B including inner hollow, can in external packing box 1B Active box 2B is releasably installed, and has the first air inlet 11B on two opposite side end faces of external packing box 1B With the first venthole 12B；First air inlet 12B is connected by pipeline with the outlet side that external cold air pumps 3B；Active box 2B Side end face on be provided with the second air inlet 21B and second outlet opposite with the first air inlet 11B and the first position venthole 12B Hole 22B；Medium position in active box 2B is equipped with partition 4B；And active box 2B be partitioned by the partition 4B it is several A cell 23B；And the inner wall of each cell 23B is both provided with locating piece 5B；It stores up bee tank 6B and passes through the locating piece 5B It is detachably disposed in active box 2B.
First air inlet 11B is arranged at the adjacent position in two side end faces of external packing box 1B；On active box 2B Second air inlet 21B is the different aperture of multiple sizes for being distributed on its side end face；And from close to far from the first air inlet The position of 12B, the size of the aperture are in the trend being gradually increased；First venthole 12B and the second venthole 22B are all disposed within Side far from the first air inlet 11B.
Locating slot 13B is provided on the inner bottom surface of external packing box 1B；It is provided with and institute in the bottom surface of active box 2B State the inserted block 24B that locating slot 12B is matched；It is also installed between bottom in external packing box 1B and active box 2B outer bottom There is cushion pad 7B；Storage bee tank 6B is cylindrical structure；The column that locating piece 5B is placed in cell 23B in four cruciform symmetries Block structure；And locating piece 5B is arcuate structure with the side to connect storage bee tank 6B.
When invention device transports microbracon hebetor in using the present embodiment, first active box 2B is inserted along positioning Slot 13B is installed in external packing box, and will be inserted into active box along locating piece 5B at the storage bee tank 6B of bee or pupa equipped with microbracon hebetor It is fixed in body 2B, then the upper top opening of external packing box is sealed；Entire external packing box is placed in transport vehicle, band dress Cold air pump 3B is gone out shortness of breath after the completion of case to connect by tracheae with the first air inlet 11B；It only needs to open cold air in transportational process The switch of pump 3B enables cold air to take a breath by the hole of device to lasting cooling is carried out inside active box 2B, to improve Microbracon hebetor survival rate during transportation.
Oviposition box includes bottle body 1 and the bottle cap being arranged on bottle body 12 used in the step S2, described The upper surface of bottle cap 2 offers air hole 3, the first gauze 4 for sealing is equipped in the air hole 3, in the bottle body 1 Be provided at below the bottleneck into insect population 5, it is described enter insect population 5 at sealed with tampon 6, offered in the bottom of the bottle body 1 Circular hole, is equipped with the second gauze 7 for sealing in the circular hole, and the lower section of the bottle body 1 is provided with collection ovum disk 8.
Using tampon 6 after cacac moth adult is transferred in oviposition box by entering insect population 5 first when using oviposition box It is sealed；The hydromel that concentration is 20% is dipped on the first gauze 4 for cacac moth imago feeding, and daily to first Gauze 4 is replaced, and is taken the ovum period depending on specifically growth duration, is generally acquired in 1-3d, uses hair when collecting ovum Pen gently brush the ovum being sticked on the second gauze 7, brush to collect ovum disk 8 on, then will collect ovum disk 8 remove be collected after again will collect Ovum disk reinstalls.
Embodiment 2:
The present invention also proposes a kind of feed of artificial feeding cacac moth larva simultaneously；
Corn sand and half wheat shorts are obtained in (60 DEG C) baking 1-3h of baking oven before formula feed, determine the gross weight of formula feed Amount, production tri- groups of experiments of A, B, C are compareed, and the weight (being shown in Table 1) of each component is weighed by corresponding proportion, and are first weighed solid Body, then liquid is weighed, after weighing all material, it is stirred for uniformly；
1 cacac moth man-made feeds formula (g) of table
According to raising in step S3 as a result, as follows to man-made feeds evaluation analysis of the invention:
(1) development duration:
The cacac moth of feeding Three feed formula, ovum phase, larval phase and pupa time development difference is not significant, especially ovum Phase difference is minimum.But ovum to the survival rate significant difference (P < 0.05) of adult, the survival rate of feed A is up to 75.91%, feed C Survival rate minimum 69.42% (table 2), but the cacac moth of Three feed raising, survival rate is all larger than high family and waits People studies the optimum formula C obtained.
Influence of the 2 Three feed formula of table to cacac moth immature phase and ovum to adult survival rate
Note: numerical value is average ± standard error.In each column different lowercases indicate there are significant difference (P < 0.05, Duncan)
(2) Life of Adult, single female egg laying amount and property ratio:
From table 3 it can be seen that Three feed formula is to cacac moth male and female Life of Adult, single female egg laying amount and female and male ratio Influence difference it is not significant.The average life span that feeding is formulated the female adult and male worm of C is respectively 10.00d and 9.00d, is above it His two kinds of feed formulas, and single female egg laying amount also highest (67.40).The cacac moth of Three feed formula raising, male and female Property ratio is close to 1:1.
Influence of the 3 Three feed formula of table to cacac moth Life of Adult, single female egg laying amount and property ratio
By result above it is known that can obtain a large amount of populations with Three feed formula raising cacac moth, but it is comprehensive Consider indexs and the economic costs such as larval phase, pupa time, survival rate, it is considered herein that feed B is the optimum formula of cacac moth, and Moroxydine Hydrochloride piece is added in the present invention can reduce the probability of larva virus infection.Therefore, the formula economic cost is low, raising The development of worm state is consistent and can obtain a large amount of adults, this is of great significance to the procreation of population.Therefore, feed B is to cacac moth Raising with good development and application prospects.

Claims (9)

1. a kind of method for killing Storage tobacco cacac moth so that cacac moth is host's feeding transport microbracon hebetor bee pupa, It is characterized in that: the following steps are included:
S1, preparation raise the man-made feeds of cacac moth larva: the man-made feeds include the component of following parts by weight: dry jade Rice sand 27-33%, dry crushing wheat 36.3-73.3%, yeast powder 5.5-8.5%, full fat soybean powder 1.2-2.8%, oatmeal 0-7%, Buddhist nun's platinum Tortoise beetle ester 0.15-0.35%, sorbic acid 0.10-0.4%, Moroxydine Hydrochloride piece 0.1-0.3%, glycerol 6-8%, honey 5-9% and water 2.5-3.5%；It is prepared by being stirred after being weighed above-mentioned material；
S2, the cacac moth adult group for the 1-3d that sprouts wings is transferred in oviposition box, the environment temperature for box of laying eggs is 27-29 DEG C, phase To humidity 70-80% and photoperiod 16L；And the bee syrup that addition concentration is 20-30% into oviposition box daily, and with the week of 1-3d Collection of the phase to ovum is carried out in oviposition box；
S3, the man-made feeds prepared in S1 are placed in the first raising box, then the ovum collected in S2 is uniformly sprinkling upon the first raising In box, and the first raising box is placed in environmental condition environment identical with oviposition box；
S4, after cacac moth egg hatching is at larva by the cacac moth larva of certain amount from first raising box be transferred to second Box is raised, and 1 pairs of generation microbracon hebetor is introduced into the second raising box at bee；Into the second raising box, addition concentration is daily The bee syrup of 10-70%；And the second raising box is placed in environmental condition environment identical with oviposition box；Deng 1 generation microbracon hebetor Cacac moth larva is carried out 2 generation microbracon hebetors can be obtained after parasitic oviposition；
S5, bee pupa is shifted from the second raising box via a length of larva of oviparity and after tying pupa after 2 generation microbracon hebetor ovum in S4 Entrucking is carried out into transport case, and guarantees that the temperature in transportation environment locating for transport case is 12-18 DEG C；
S6, microbracon hebetor in transport case bee pupa sprout wings before transport case is transported in the warehouse for having tobacco leaf to storage, and will The bee pupa of microbracon hebetor in transport case is transferred in bee-escape；Microbracon hebetor sprout wings after can by microbracon hebetor at bee to storehouse Cacac moth progress parasitism is killed on storage tobacco leaf.
2. a kind of raised with cacac moth for host according to claim 1 transports microbracon hebetor bee pupa to kill storage cigarette The method of leaf cacac moth, it is characterised in that: the number of cacac moth larva and pairs of microbracon hebetor in the step S4 Logarithm ratio 10:1.
3. a kind of raised with cacac moth for host according to claim 1 or 2 transports microbracon hebetor bee pupa to kill storehouse The method for storing up tobacco leaf cacac moth, it is characterised in that: it is 10cm that the size of the second raising box, which is basal diameter, a height of 8cm Cylinder box body structure.
4. a kind of raised with cacac moth for host according to claim 1 transports microbracon hebetor bee pupa to kill storage cigarette The method of leaf cacac moth, it is characterised in that: the second raising box used in the step S4 includes tank body (1A)；Tank body (1A) is the column structure of both ends open inner hollow；And it is removably installed top cover (2A) respectively at the both ends of tank body (1A) With bottom cover (3A)；It is provided with ventilation through-hole (21A) and location hole (22A) on top cover (2A), and is covered in ventilation through-hole (21A) There is separation net (6A)；The inner cavity tank body (1A) is inserted by location hole (22A) in one end of nutrition liquid pipe (7A)；At nutrition liquid pipe (7A) Lower bottom part be fixed with cotton mass (8A)；Bottom cover (3A) includes the mounting disc (31A) being connected with tank body (1A)；In mounting disc The end face opposite with the inner cavity of tank body (1A) is provided with connector (32A) on (31A)；Pacify on the other end of connector (32A) Equipped with catch tray (33A)；The size of catch tray (33A) and the inner wall size of tank body (1A) match.
5. a kind of raised with cacac moth for host according to claim 4 transports microbracon hebetor bee pupa to kill storage cigarette The method of leaf cacac moth, it is characterised in that: have external thread section (4A) at the both ends of tank body (1A), top cover (2A) with The internal thread segment (5A) to match with the external thread section is provided on bottom cover (3A)；Mounting disc (31A) on bottom cover (3A) is band There is the integral structure of ring wall and disk；Internal thread segment (5A) is arranged on the ring wall；The connector position (32A) is arranged in the circle The rod-like structure at the middle part of disk；Mounting groove (331A) is provided on the side wall of catch tray (33A)；And on the mounting groove (331A) Sealing ring (34A) is installed；Once mild-natured gasket (332A) is removably installed in the upper surface of catch tray (33A).
6. a kind of raised with cacac moth for host according to claim 1 transports microbracon hebetor bee pupa to kill storage cigarette The method of leaf cacac moth, it is characterised in that: the transport case used in the step S5；External packing box including inner hollow (1B) has been removably installed active box (2B) in external packing box (1B), and in two opposite sides of external packing box (1B) The first air inlet (11B) and the first venthole (12B) are had on end face；First air inlet (12B) by pipeline with it is external The outlet side of cold air pump (3B) is connected；It is provided on the side end face of active box (2B) and goes out with the first air inlet (11B) and first The position stomata (12B) opposite the second air inlet (21B) and the second venthole (22B)；Middle position in active box (2B) It sets and partition (4B) is installed；And active box (2B) is partitioned into several cells (23B) by the partition (4B)；And each list The inner wall of first lattice (23B) is both provided with locating piece (5B)；Storage bee tank (6B) is detachably disposed to by the locating piece (5B) In active box (2B).
7. a kind of raised with cacac moth for host according to claim 6 transports microbracon hebetor bee pupa to kill storage cigarette The method of leaf cacac moth, it is characterised in that: the first air inlet (11B) setting is adjacent in two side end faces of external packing box (1B) Position at；The second air inlet (21B) on active box (2B) is different small of multiple sizes for being distributed on its side end face Hole；And from close to the position far from the first air inlet (12B), the size of the aperture is in the trend being gradually increased；First goes out Stomata (12B) and the second venthole (22B) are all disposed within the side far from the first air inlet (11B).
8. a kind of raised with cacac moth for host according to claim 6 transports microbracon hebetor bee pupa to kill storage cigarette The method of leaf cacac moth, it is characterised in that: locating slot (13B) is provided on the inner bottom surface of external packing box (1B)；In activity The bottom surface of cabinet (2B) is provided with the inserted block (24B) matched with the locating slot (12B)；At the interior bottom external packing box (1B) Cushion pad (7B) is also equipped between portion and active box (2B) outer bottom；Storing up bee tank (6B) is cylindrical structure；Locating piece The column block structure that (5B) is placed in cell (23B) in four cruciform symmetries；And locating piece (5B) and storage bee tank (6B) phase The side connect is arcuate structure.
9. a kind of raised with cacac moth for host according to claim 1 transports microbracon hebetor bee pupa to kill storage cigarette The method of leaf cacac moth, it is characterised in that: the oviposition box used in the step S2 includes that bottle body (1) and setting exist Bottle cap (2) on bottle body (1) offers air hole (3) in the upper surface of the bottle cap (2), is equipped in the air hole (3) For the first gauze (4) of sealing, be located at bottleneck lower section in the bottle body (1) and be provided with into insect population (5), it is described enter insect population (5) it is sealed at tampon (6), offers circular hole in the bottom of the bottle body (1), for sealing is equipped in the circular hole Two gauzes (7) are provided with collection ovum disk (8) below the bottle body (1).
CN201910489263.6A 2019-06-06 2019-06-06 A method of Storage tobacco cacac moth is killed so that cacac moth is host's feeding transport microbracon hebetor bee pupa Pending CN110235858A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201910489263.6A CN110235858A (en) 2019-06-06 2019-06-06 A method of Storage tobacco cacac moth is killed so that cacac moth is host's feeding transport microbracon hebetor bee pupa

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201910489263.6A CN110235858A (en) 2019-06-06 2019-06-06 A method of Storage tobacco cacac moth is killed so that cacac moth is host's feeding transport microbracon hebetor bee pupa

Publications (1)

Publication Number Publication Date
CN110235858A true CN110235858A (en) 2019-09-17

Family

ID=67886333

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201910489263.6A Pending CN110235858A (en) 2019-06-06 2019-06-06 A method of Storage tobacco cacac moth is killed so that cacac moth is host's feeding transport microbracon hebetor bee pupa

Country Status (1)

Country Link
CN (1) CN110235858A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110506711A (en) * 2019-09-29 2019-11-29 福建农林大学 A kind of parasitic wasp breeding apparatus and its application method

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1832165A1 (en) * 2004-11-26 2007-09-12 Kyoto University Feeding method and feeding device for raising natural enemy insect and method of raising natural enemy insect
CN207461232U (en) * 2017-09-25 2018-06-08 广西壮族自治区农业科学院甘蔗研究所 A kind of multi-functional rice moth breeding apparatus
CN208217309U (en) * 2018-04-16 2018-12-11 长江大学 A kind of roundheaded borer long-distance transport damping storage apparatus
CN208434568U (en) * 2017-05-17 2019-01-29 贵州省烟草公司毕节市公司 A kind of Novel tobacco grass meal snout moth's larva oviposition collection device
CN208639370U (en) * 2018-08-22 2019-03-26 河南省农业科学院烟草研究所 A kind of aphid parasite deadlock aphid release box

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1832165A1 (en) * 2004-11-26 2007-09-12 Kyoto University Feeding method and feeding device for raising natural enemy insect and method of raising natural enemy insect
CN208434568U (en) * 2017-05-17 2019-01-29 贵州省烟草公司毕节市公司 A kind of Novel tobacco grass meal snout moth's larva oviposition collection device
CN207461232U (en) * 2017-09-25 2018-06-08 广西壮族自治区农业科学院甘蔗研究所 A kind of multi-functional rice moth breeding apparatus
CN208217309U (en) * 2018-04-16 2018-12-11 长江大学 A kind of roundheaded borer long-distance transport damping storage apparatus
CN208639370U (en) * 2018-08-22 2019-03-26 河南省农业科学院烟草研究所 A kind of aphid parasite deadlock aphid release box

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
石巍: "《无公害蜂产品生产技术》", 28 February 2004, 金盾出版社 *
胡涌等: "贵州烟仓害虫天敌种类及优势天敌初步研究 ", 《植物保护》 *
袁敏等: "烟草粉螟对5种杀虫剂的敏感性测定", 《山地农业生物学报》 *
陈浩梁: ""麦蛾柔茧蜂规模化人工饲养的关键技术及其冷胁迫的反应机制"", 《中国博士学位论文全文数据库农业科技辑》 *
陕西省林业科学研究所: "《林虫寄生蜂图志》", 28 February 1990, 天泽出版社 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110506711A (en) * 2019-09-29 2019-11-29 福建农林大学 A kind of parasitic wasp breeding apparatus and its application method
CN110506711B (en) * 2019-09-29 2023-12-29 福建农林大学 Parasitic wasp feeding device and using method thereof

Similar Documents

Publication Publication Date Title
CN103155906A (en) Aphidoletes aphidimyza mass reproduction technology and application technology
CN112868606B (en) Method for indoor raising sugarcane striped rice borers or dibbler
CN105746435B (en) A kind of method using rice moth egg breeding chrysopa septempunctata larva
CN108077191A (en) A kind of Spodoptera litura larvae group breeding method
CN106900656B (en) A kind of cigarette fleahopper industrial production method
CN102499188B (en) Agasicles hygrophila experimental population raising method
CN105379683B (en) A kind of gelechiid rearing method
CN106879557A (en) A kind of method for raising black striped plant bug
CN110150233B (en) Method for killing stored tobacco meal moth by raising adult braconid flies of wheat moth braconid flies in batch
CN108041353A (en) A kind of dichocrocis punctiferalis man-made feeds and its preparation method and application
CN102550490B (en) Method of raising ectropis oblique warren through sambucus chinensis and Ectropis grisescens nucleopolyhedrovirus (EcobNPV) propagation method utilizing same
CN110235858A (en) A method of Storage tobacco cacac moth is killed so that cacac moth is host&#39;s feeding transport microbracon hebetor bee pupa
CN103704178B (en) A kind of regulate and control full skirt chase after the method for posting fly diapause
CN109907007A (en) A kind of device and method of large-scale breeding cacac moth
CN202232599U (en) Device for feeding lepidoptera insect larvae indoors
CN110235857A (en) A method of transport microbracon hebetor is raised for host with cacac moth and kills Storage tobacco cacac moth at bee
CN110150232A (en) A kind of method that batch raising microbracon hebetor bee pupa kills storage cacac moth
CN111066732B (en) Method for breeding orius sauteri
CN110235859A (en) A kind of method of artificial batch feeding packaging microbracon hebetor living body bee pupa
CN106172251A (en) A kind of method for breeding of Mythimna Lorgi
Sterling et al. An artificial diet for laboratory cultures of boll weevil larvae and adults
CN109769757A (en) The kind winged type insect breeding apparatus of one kind and method for breeding
CN110367203B (en) Method for artificially breeding packaged live adult wheat moth cocoon bees in batch
CN109247306B (en) Tobacco beetle feeding and separating device and male and female adult separation method using same
CN109548755A (en) A kind of method for breeding of striped rice borer

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication
RJ01 Rejection of invention patent application after publication

Application publication date: 20190917