CN110235859A - A kind of method of artificial batch feeding packaging microbracon hebetor living body bee pupa - Google Patents
A kind of method of artificial batch feeding packaging microbracon hebetor living body bee pupa Download PDFInfo
- Publication number
- CN110235859A CN110235859A CN201910489390.6A CN201910489390A CN110235859A CN 110235859 A CN110235859 A CN 110235859A CN 201910489390 A CN201910489390 A CN 201910489390A CN 110235859 A CN110235859 A CN 110235859A
- Authority
- CN
- China
- Prior art keywords
- box
- bee
- microbracon
- microbracon hebetor
- hebetor
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000985295 Habrobracon hebetor Species 0.000 title claims abstract description 59
- 241000382353 Pupa Species 0.000 title claims abstract description 23
- 238000000034 method Methods 0.000 title claims abstract description 20
- 238000004806 packaging method and process Methods 0.000 title claims abstract description 16
- 238000012856 packing Methods 0.000 claims abstract description 37
- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 28
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 28
- 210000004681 ovum Anatomy 0.000 claims abstract description 28
- 241000238631 Hexapoda Species 0.000 claims abstract description 11
- 239000000843 powder Substances 0.000 claims abstract description 7
- 230000017448 oviposition Effects 0.000 claims description 30
- 239000007788 liquid Substances 0.000 claims description 12
- 235000016709 nutrition Nutrition 0.000 claims description 11
- 230000035764 nutrition Effects 0.000 claims description 11
- 210000004027 cell Anatomy 0.000 claims description 10
- 238000007789 sealing Methods 0.000 claims description 10
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 9
- 238000003860 storage Methods 0.000 claims description 9
- 235000013601 eggs Nutrition 0.000 claims description 8
- 238000009423 ventilation Methods 0.000 claims description 8
- 241000209140 Triticum Species 0.000 claims description 7
- 235000021307 Triticum Nutrition 0.000 claims description 7
- 230000007613 environmental effect Effects 0.000 claims description 7
- 238000005192 partition Methods 0.000 claims description 7
- 239000006188 syrup Substances 0.000 claims description 7
- 235000020357 syrup Nutrition 0.000 claims description 7
- 229920000742 Cotton Polymers 0.000 claims description 5
- 230000003071 parasitic effect Effects 0.000 claims description 5
- BITDLWAQPKSXTF-UHFFFAOYSA-M 1-[(3-nitrophenyl)methoxymethyl]pyridin-1-ium;chloride Chemical compound [Cl-].[O-][N+](=O)C1=CC=CC(COC[N+]=2C=CC=CC=2)=C1 BITDLWAQPKSXTF-UHFFFAOYSA-M 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 4
- 229960002581 moroxydine hydrochloride Drugs 0.000 claims description 4
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 claims description 4
- 239000004576 sand Substances 0.000 claims description 4
- 238000000926 separation method Methods 0.000 claims description 4
- 244000068988 Glycine max Species 0.000 claims description 3
- 235000010469 Glycine max Nutrition 0.000 claims description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 3
- BEFDCLMNVWHSGT-UHFFFAOYSA-N ethenylcyclopentane Chemical compound C=CC1CCCC1 BEFDCLMNVWHSGT-UHFFFAOYSA-N 0.000 claims description 3
- 230000012447 hatching Effects 0.000 claims description 3
- 235000012907 honey Nutrition 0.000 claims description 3
- 229910052697 platinum Inorganic materials 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- 229940075582 sorbic acid Drugs 0.000 claims description 3
- 235000010199 sorbic acid Nutrition 0.000 claims description 3
- 239000004334 sorbic acid Substances 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 241000162403 Deloyala guttata Species 0.000 claims 1
- 240000007594 Oryza sativa Species 0.000 claims 1
- 235000007164 Oryza sativa Nutrition 0.000 claims 1
- 150000002148 esters Chemical class 0.000 claims 1
- 239000010977 jade Substances 0.000 claims 1
- 235000009566 rice Nutrition 0.000 claims 1
- 238000005516 engineering process Methods 0.000 abstract description 3
- 238000009395 breeding Methods 0.000 abstract description 2
- 230000001488 breeding effect Effects 0.000 abstract description 2
- 241000208125 Nicotiana Species 0.000 abstract 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 abstract 1
- 210000004894 snout Anatomy 0.000 abstract 1
- 230000004083 survival effect Effects 0.000 description 8
- 238000011161 development Methods 0.000 description 4
- 230000018109 developmental process Effects 0.000 description 4
- 240000008042 Zea mays Species 0.000 description 3
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 3
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 3
- 235000005822 corn Nutrition 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 230000024241 parasitism Effects 0.000 description 3
- 241000607479 Yersinia pestis Species 0.000 description 2
- 230000003139 buffering effect Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000001418 larval effect Effects 0.000 description 2
- -1 platinum methyl esters Chemical class 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000032258 transport Effects 0.000 description 2
- 244000025254 Cannabis sativa Species 0.000 description 1
- 208000000059 Dyspnea Diseases 0.000 description 1
- 206010013975 Dyspnoeas Diseases 0.000 description 1
- 241000255777 Lepidoptera Species 0.000 description 1
- 241000256259 Noctuidae Species 0.000 description 1
- 241000255893 Pyralidae Species 0.000 description 1
- 241001340903 Pyralis farinalis Species 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000019504 cigarettes Nutrition 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 244000078703 ectoparasite Species 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000000384 rearing effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 208000013220 shortness of breath Diseases 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000009385 viral infection Effects 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/111—Aromatic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/116—Heterocyclic compounds
- A23K20/137—Heterocyclic compounds containing two hetero atoms, of which at least one is nitrogen
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D81/00—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents
- B65D81/18—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents providing specific environment for contents, e.g. temperature above or below ambient
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D85/00—Containers, packaging elements or packages, specially adapted for particular articles or materials
- B65D85/50—Containers, packaging elements or packages, specially adapted for particular articles or materials for living organisms, articles or materials sensitive to changes of environment or atmospheric conditions, e.g. land animals, birds, fish, water plants, non-aquatic plants, flower bulbs, cut flowers or foliage
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Food Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Environmental Sciences (AREA)
- Mechanical Engineering (AREA)
- Marine Sciences & Fisheries (AREA)
- General Health & Medical Sciences (AREA)
- Evolutionary Biology (AREA)
- Toxicology (AREA)
- Animal Behavior & Ethology (AREA)
- Botany (AREA)
- Mycology (AREA)
- Insects & Arthropods (AREA)
- Birds (AREA)
- Biodiversity & Conservation Biology (AREA)
- Biochemistry (AREA)
- Jellies, Jams, And Syrups (AREA)
- Catching Or Destruction (AREA)
Abstract
The invention discloses a kind of artificial methods of batch feeding packaging microbracon hebetor living body bee pupa, are related to insect breeding apparatus technical field, it is the following steps are included: S1, configure the feed of cacac moth；S2, growing environment is provided for cacac moth and periodically cacac moth ovum is collected；The feed that S1 is deployed is added into the first raising box in S3, transfer tobacco powder snout moth's larva ovum thereto；S4, will hatch later cacac moth larva be transferred in the second raising box and to second raising box in be put into 1 pairs of generation microbracon hebetor into bee；S5, it is collected after 2 generation microbracon hebetors become bee pupa from ovum, larva from the second raising box, and transfers them in packing box progress entrucking processing；The present invention efficiently solves currently still immature to the artificial feeding technology of microbracon hebetor, the problem of all lacking effective technological means in the feeding of the host of microbracon hebetor ovipositing hosts and the collecting packing of microbracon hebetor living body bee pupa to meet batch acquisition microbracon hebetor bee pupa.
Description
Technical field
The present invention relates to insect breeding apparatus technical field, and in particular to a kind of artificial batch feeding packaging microbracon hebetor
The method of living body bee pupa.
Background technique
Microbracon hebetor is a kind of ectoparasite bee, is a kind of important parasitics world of lepidoptera pest in field and warehouse,
Its host range is widely distributed, relates generally to field and warehouse Lepidoptera Noctuidae and Pyralidae；Due to the parasitism of microbracon hebetor
Ability is strong, and reproduction speed is fast, and control of insect is had a good application prospect, this pest of cacac moth is directed to by it and
Speech, it is existing research shows that the parasitism that microbracon hebetor can effectively carry out cacac moth is killed, to play the effect of biological control
Fruit；Be directed to microbracon hebetor at present even rests on the experimental stage using the country, and tracing it to its cause is to the artificial of microbracon hebetor
Rearing Technology is still immature, and technological difficulties therein concentrate on the feeding of the host to microbracon hebetor ovipositing hosts, and
On the collecting packing of microbracon hebetor living body bee pupa；So a kind of skill for being able to solve batch feeding packaging microbracon hebetor living body bee pupa
Art method is exactly urgently needed at present
Summary of the invention
The technical problems to be solved by the present invention are: providing a kind of manually raise in batches packs microbracon hebetor living body into bee
Method, to solve currently still immature to the artificial feeding technology of microbracon hebetor host especially in microbracon hebetor ovipositing hosts
Feeding and microbracon hebetor living body bee pupa collecting packing on all lack effective technological means meet batch raising gelechiid
The problem of cocoon bee.
To solve the above problems, the present invention provides the following technical scheme that
A kind of method of artificial batch feeding packaging microbracon hebetor living body bee pupa, comprising the following steps:
S1, preparation raise the man-made feeds of cacac moth larva: the man-made feeds include the component of following parts by weight:
Dry corn sand 27-33%, dry crushing wheat 36.3-73.3%, yeast powder 5.5-8.5%, full fat soybean powder 1.2-2.8%, oatmeal
0-7%, Buddhist nun's platinum methyl esters 0.15-0.35%, sorbic acid 0.10-0.4%, Moroxydine Hydrochloride piece 0.1-0.3%, glycerol 6-8%,
Honey 5-9% and water 2.5-3.5%；It is prepared by being stirred after being weighed above-mentioned material；
S2, the cacac moth adult group for the 1-3d that sprouts wings is transferred in oviposition box, the environment temperature for box of laying eggs is 27-29
DEG C, relative humidity 70-80% and photoperiod 16L；And the bee syrup that addition concentration is 20-30% into oviposition box daily, and with
Collection of the period of 1-3d to ovum is carried out in oviposition box；
S3, the man-made feeds prepared in S1 are placed in the first raising box, then the ovum collected in S2 is uniformly sprinkling upon first
It raises in box, and the first raising box is placed in environmental condition environment identical with oviposition box；
S4, after cacac moth egg hatching is at larva by the cacac moth larva of certain amount from first raising box be transferred to
Second raising box, and 1 pairs of generation microbracon hebetor is introduced into the second raising box at bee；It is added daily into the second raising box dense
Degree is the bee syrup of 10-70%；And the second raising box is placed in environmental condition environment identical with oviposition box；Deng 1 generation wheat
Moth cocoon bee carries out cacac moth larva 2 generation microbracon hebetors can be obtained after parasitic oviposition；
S5, after 2 generation microbracon hebetor ovum in S4 via a length of larva of oviparity and after tying pupa by bee pupa from the second raising box
It is transferred in packing box and carries out entrucking, and guarantee that the temperature in transportation environment locating for packing box is 12-18 DEG C.
Preferably, in the step S2 number of cacac moth larva and pairs of microbracon hebetor logarithm ratio 10:1.
Preferably, it is 10cm that the size of the second raising box, which is basal diameter, the cylinder box body structure of a height of 8cm.
Preferably, the second raising box used in the step S4 includes tank body；Tank body is both ends open inner hollow
Column structure；And it is removably installed top cover and bottom cover respectively at the both ends of tank body；Be provided on top cover ventilation through-hole and
Location hole, and separation net is covered on ventilation through-hole；Tank inner chamber is inserted by location hole in one end of nutrition liquid pipe；In nutrition
The lower bottom part of liquid pipe is fixed with cotton mass；Bottom cover includes the mounting disc being connected with tank body；In mounting disc with the inner cavity phase of tank body
Pair end face be provided with connector；Catch tray is installed on the other end of the connector；The size of catch tray and the inner wall of tank body
Size matches.
Further, external thread section is had at the both ends of tank body, is provided with and the external screw thread on top cover and bottom cover
The internal thread segment that section matches；Mounting disc on bottom cover is the integral structure with ring wall and disk；Internal thread segment is arranged in institute
It states on ring wall；The rod-like structure at the middle part of the disk is arranged in connector position；Mounting groove is provided on the side wall of catch tray；And
Sealing ring is installed on the mounting groove；Once mild-natured gasket is removably installed in the upper surface of catch tray.
Preferably, the packing box used in the step S5；External packing box including inner hollow, in external packing box
It has been removably installed active box, and has had the first air inlet and first on two opposite side end faces of external packing box
Venthole；First air inlet is connected by pipeline with the outlet side that external cold air pumps；Be provided on the side end face of active box with
Opposite the second air inlet and the second venthole of first air inlet and the first outlet hole site；In the intracorporal medium position of active box
Partition is installed；And active box is partitioned into several cells by the partition；And the inner wall of each cell is both provided with
Locating piece；Storage bee tank is detachably disposed in active box by the locating piece.
Further, the first air inlet is arranged at the adjacent position in two side end faces of external packing box；On active box
The second air inlet be the different aperture of multiple sizes for being distributed on its side end face；And from close to far from the first air inlet
Position, the size of the aperture are in the trend being gradually increased；First venthole and the second venthole be all disposed within far from first into
The side of stomata.
Further, locating slot is provided on the inner bottom surface of external packing box；The bottom surface of active box be provided with
The inserted block that the locating slot matches；It is soft that buffering is also equipped between bottom in external packing box and active box outer bottom
Pad；Storage bee tank is cylindrical structure；The column block structure that locating piece is placed in cell in four cruciform symmetries；And locating piece
The side to connect with storage bee tank is arcuate structure.
Preferably, the oviposition box used in the step S2 includes bottle body and the bottle cap that is arranged on bottle body,
The upper surface of the bottle cap offers air hole, the first gauze for sealing is equipped in the air hole, in the bottle body
Be provided at below the bottleneck into insect population, it is described enter insect population at sealed with tampon, offer circular hole in the bottom of the bottle body,
It is equipped with the second gauze for sealing in the circular hole, collection ovum disk is provided with below the bottle body.
The invention has the advantages that:
The beneficial effects of the present invention are embodied in the following aspects: 1, by configuring the feed of cacac moth and providing its life
Environmental condition needed for long oviposition is efficiently solved to be difficult to find during cultivating microbracon hebetor suitably to lay eggs and be posted
It is main, thus the problem of can not cultivating in batches；2, reliable ring is provided for growth courses such as the 1 normal mate and oviposits of generation microbracon hebetor
Border condition improves the cacac moth larva as host by parasitic rate, to increase the number for cultivating 2 generation microbracon hebetors；Another party
Face, the present invention also provides can be improved the life cycle for improving microbracon hebetor and the packaging for capableing of batch package microbracon hebetor
Box, thus make the client of different zones can obtain using；And the oviposition box laid eggs convenient for cacac moth, raise wheat
The raising box of moth cocoon bee, it is inconvenient to solve the collection faced in the specific implementation process, at the lower problem of bee survival rate.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the second raising box in the present invention；
Fig. 2 is the structural schematic diagram of packing box in the present invention；
Fig. 3 is the A direction view of Fig. 2；
Fig. 4 is the structural schematic diagram of oviposition box in the present invention；
Description of symbols: 1, bottle body, 2, bottle cap, 3, air hole, the 4, first gauze, 5, enter insect population, 6, tampon；7,
Two gauzes, 8, collection ovum disk；
1A, tank body, 2A, top cover, 3A, bottom cover, 4A, external thread section, 5A, internal thread segment, 6A, separation net, 7A, nutrition liquid pipe
A, 8A, cotton mass, 11A, auxiliary ventilation through-hole, 21A, ventilation through-hole, 22A, location hole, 31A, mounting disc, 32A, connector,
33A, catch tray, 34A, sealing ring, 331A, mounting groove, 332A, disposable flat shim；
1B, external packing box, 2B, active box, 3B, cold air pump, 4B, partition, 5B, locating piece, 6B, storage bee tank, 7B, buffering
Cushion, 11B, the first air inlet, 12B, the first venthole, 13B, locating slot, 21B, the second air inlet, 22B, the second outlet
Hole, 23B, cell, 24B, inserted block.
Specific embodiment
With reference to the accompanying drawing and the present invention is described further in specific embodiment:
Embodiment:
Referring to Fig.1, the present embodiment provides a kind of artificial batches to raise the method for packing microbracon hebetor living body bee pupa, including
Following steps:
S1, preparation raise the man-made feeds of cacac moth larva: the man-made feeds include the component of following parts by weight:
Dry corn sand 28%, dry crushing wheat 42.3%, yeast powder 7%, full fat soybean powder 2.5%, oatmeal 3%, Buddhist nun's platinum methyl esters
0.25%, sorbic acid 0.25%, Moroxydine Hydrochloride piece 0.2%, glycerol 7%, honey 6.5% and water 3%；Its prepare pass through by
Above-mentioned material is stirred after being weighed；
S2, the cacac moth adult group for the 1-3d that sprouts wings being transferred in oviposition box, the environment temperature for box of laying eggs is 28 DEG C,
Relative humidity 75% and photoperiod 16L；And the bee syrup that addition concentration is 25% into oviposition box daily, and with the period of 1-3d
Collection to ovum is carried out in oviposition box；
S3, the man-made feeds prepared in S1 are placed in the first raising box, then the ovum collected in S2 is uniformly sprinkling upon first
It raises in box, and the first raising box is placed in environmental condition environment identical with oviposition box；
S4, after cacac moth egg hatching is at larva by the cacac moth larva of certain amount from first raising box be transferred to
Second raising box, and 1 pairs of generation microbracon hebetor is introduced into the second raising box at bee；It is added daily into the second raising box dense
Degree is the bee syrup of 10-70%；And the second raising box is placed in environmental condition environment identical with oviposition box；Deng 1 generation wheat
Moth cocoon bee carries out cacac moth larva 2 generation microbracon hebetors can be obtained after parasitic oviposition；
S5, after 2 generation microbracon hebetor ovum in S4 via a length of larva of oviparity and after tying pupa by bee pupa from the second raising box
It is transferred in packing box and carries out entrucking, and guarantee that the temperature in transportation environment locating for packing box is 12-18 DEG C.
The logarithm ratio 10:1 of the number of cacac moth larva and pairs of microbracon hebetor in the step S2.
It is 10cm that the size of the second raising box, which is basal diameter, the cylinder box body structure of a height of 8cm.
The second raising box used in the step S4 includes tank body 1A；Tank body 1A is the column of both ends open inner hollow
Shape structure；And it is removably installed top cover 2A and bottom cover 3A respectively at the both ends of tank body 1A；It is logical that ventilation is provided on top cover 2A
Hole 21A and location hole 22A, and separation net 6A is covered on ventilation through-hole 21A；One end of nutrition liquid pipe 7A passes through location hole
22A is inserted into the inner cavity tank body 1A；Cotton mass 8A is fixed in the lower bottom part of nutrition liquid pipe 7A；Bottom cover 3A includes being connected with tank body 1A
Mounting disc 31A；The end face opposite with the inner cavity of tank body 1A is provided with connector 32A on mounting disc 31A；In connector 32A
The other end on catch tray 33A is installed；The size of catch tray 33A and the inner wall size of tank body 1A match.
External thread section 4A is had at the both ends of tank body 1A, is provided with and the external thread section on top cover 2A and bottom cover 3A
The internal thread segment 5A to match；Mounting disc 31A on bottom cover 3A is the integral structure with ring wall and disk；Internal thread segment 5A is set
It sets on the ring wall；The rod-like structure at the connector 32A middle parts that the disk is set；It is opened on the side wall of catch tray 33A
There is mounting groove 331A；And sealing ring 34A is installed on the mounting groove 331A；The upper surface of catch tray 33A removably
Disposable flat shim 332A is installed.
During device raises microbracon hebetor and collects bee pupa in using the present embodiment, first disposable
Flat shim 332A is placed on catch tray 33A, will be placed with nutrition liquid pipe 7A along location hole 22A, and in nutrition liquid pipe 7A
The installation of the side end inside tank body 1A cotton mass is protruded into, and by bottom cover 3A and tank body 1A by being threadably mounted at together, by cigarette
Grass meal snout moth's larva larva is put into tank body 1A, is introducing pairs of 1A thereto for microbracon hebetor adult, is having nutrition liquid pipe for above-mentioned
The bottom cover 2A of 7A is installed at the top of tank body 1A and is fixed；Device, which is placed in proper environment condition, makes it be normally carried out growth
Activity, by being put into 20% bee syrup inside nutrition liquid pipe 7A timing phase tank body 1A to supplement the nutrients, in 1 generation microbracon hebetor by 2
It is produced in cacac moth larva for microbracon hebetor ovum parasitism, and 2 generation microbracon hebetors by ovum, larva and dismantle bottom cover after cocooing
3A takes out disposable flat shim 332A, and collection is completed using bee pupa under brush brush.
The packing box used in the step S5；External packing box 1B including inner hollow, can in external packing box 1B
Active box 2B is releasably installed, and has the first air inlet 11B on two opposite side end faces of external packing box 1B
With the first venthole 12B；First air inlet 12B is connected by pipeline with the outlet side that external cold air pumps 3B；Active box 2B
Side end face on be provided with the second air inlet 21B and second outlet opposite with the first air inlet 11B and the first position venthole 12B
Hole 22B；Medium position in active box 2B is equipped with partition 4B；And active box 2B be partitioned by the partition 4B it is several
A cell 23B；And the inner wall of each cell 23B is both provided with locating piece 5B；It stores up bee tank 6B and passes through the locating piece 5B
It is detachably disposed in active box 2B.
First air inlet 11B is arranged at the adjacent position in two side end faces of external packing box 1B；On active box 2B
Second air inlet 21B is the different aperture of multiple sizes for being distributed on its side end face；And from close to far from the first air inlet
The position of 12B, the size of the aperture are in the trend being gradually increased；First venthole 12B and the second venthole 22B are all disposed within
Side far from the first air inlet 11B.
Locating slot 13B is provided on the inner bottom surface of external packing box 1B；It is provided with and institute in the bottom surface of active box 2B
State the inserted block 24B that locating slot 12B is matched；It is also installed between bottom in external packing box 1B and active box 2B outer bottom
There is cushion pad 7B；Storage bee tank 6B is cylindrical structure；The column that locating piece 5B is placed in cell 23B in four cruciform symmetries
Block structure；And locating piece 5B is arcuate structure with the side to connect storage bee tank 6B.
When invention device transports microbracon hebetor in using the present embodiment, first active box 2B is inserted along positioning
Slot 13B is installed in external packing box, and will be inserted into active box along locating piece 5B at the storage bee tank 6B of bee or pupa equipped with microbracon hebetor
It is fixed in body 2B, then the upper top opening of external packing box is sealed；Entire external packing box is placed in transport vehicle, band dress
Cold air pump 3B is gone out shortness of breath after the completion of case to connect by tracheae with the first air inlet 11B；It only needs to open cold air in transportational process
The switch of pump 3B enables cold air to take a breath by the hole of device to lasting cooling is carried out inside active box 2B, to improve
Microbracon hebetor survival rate during transportation.
Oviposition box includes bottle body 1 and the bottle cap being arranged on bottle body 12 used in the step S2, described
The upper surface of bottle cap 2 offers air hole 3, the first gauze 4 for sealing is equipped in the air hole 3, in the bottle body 1
Be provided at below the bottleneck into insect population 5, it is described enter insect population 5 at sealed with tampon 6, offered in the bottom of the bottle body 1
Circular hole, is equipped with the second gauze 7 for sealing in the circular hole, and the lower section of the bottle body 1 is provided with collection ovum disk 8.
Using tampon 6 after cacac moth adult is transferred in oviposition box by entering insect population 5 first when using oviposition box
It is sealed；The hydromel that concentration is 20% is dipped on the first gauze 4 for cacac moth imago feeding, and daily to first
Gauze 4 is replaced, and is taken the ovum period depending on specifically growth duration, is generally acquired in 1-3d, uses hair when collecting ovum
Pen gently brush the ovum being sticked on the second gauze 7, brush to collect ovum disk 8 on, then will collect ovum disk 8 remove be collected after again will collect
Ovum disk reinstalls.
Embodiment 2:
The present invention also proposes a kind of feed of artificial feeding cacac moth larva simultaneously；
Corn sand and half wheat shorts are obtained in (60 DEG C) baking 1-3h of baking oven before formula feed, determine the gross weight of formula feed
Amount, production tri- groups of experiments of A, B, C are compareed, and the weight (being shown in Table 1) of each component is weighed by corresponding proportion, and are first weighed solid
Body, then liquid is weighed, after weighing all material, it is stirred for uniformly；
1 cacac moth man-made feeds formula (g) of table
According to raising in step S3 as a result, as follows to man-made feeds evaluation analysis of the invention:
(1) development duration:
The cacac moth of feeding Three feed formula, ovum phase, larval phase and pupa time development difference is not significant, especially ovum
Phase difference is minimum.But ovum to the survival rate significant difference (P < 0.05) of adult, the survival rate of feed A is up to 75.91%, feed C
Survival rate minimum 69.42% (table 2), but the cacac moth of Three feed raising, survival rate is all larger than high family and waits
People studies the optimum formula C obtained.
Influence of the 2 Three feed formula of table to cacac moth immature phase and ovum to adult survival rate
Note: numerical value is average ± standard error.In each column different lowercases indicate there are significant difference (P < 0.05,
Duncan)
(2) Life of Adult, single female egg laying amount and property ratio:
From table 3 it can be seen that Three feed formula is to cacac moth male and female Life of Adult, single female egg laying amount and female and male ratio
Influence difference it is not significant.The average life span that feeding is formulated the female adult and male worm of C is respectively 10.00d and 9.00d, is above it
His two kinds of feed formulas, and single female egg laying amount also highest (67.40).The cacac moth of Three feed formula raising, male and female
Property ratio is close to 1:1.
Influence of the 3 Three feed formula of table to cacac moth Life of Adult, single female egg laying amount and property ratio
By result above it is known that can obtain a large amount of populations with Three feed formula raising cacac moth, but it is comprehensive
Consider indexs and the economic costs such as larval phase, pupa time, survival rate, it is considered herein that feed B is the optimum formula of cacac moth, and
Moroxydine Hydrochloride piece is added in the present invention can reduce the probability of larva virus infection.Therefore, the formula economic cost is low, raising
The development of worm state is consistent and can obtain a large amount of adults, this is of great significance to the procreation of population.Therefore, feed B is to cacac moth
Raising with good development and application prospects.
Claims (9)
1. a kind of artificial batch feeding packaging microbracon hebetor living body is at the method for bee, it is characterised in that: the following steps are included:
S1, preparation raise the man-made feeds of cacac moth larva: the man-made feeds include the component of following parts by weight: dry jade
Rice sand 27-33%, dry crushing wheat 36.3-73.3%, yeast powder 5.5-8.5%, full fat soybean powder 1.2-2.8%, oatmeal 0-7%, Buddhist nun's platinum
Tortoise beetle ester 0.15-0.35%, sorbic acid 0.10-0.4%, Moroxydine Hydrochloride piece 0.1-0.3%, glycerol 6-8%, honey 5-9% and water
2.5-3.5%；It is prepared by being stirred after being weighed above-mentioned material；
S2, the cacac moth adult group for the 1-3d that sprouts wings is transferred in oviposition box, the environment temperature for box of laying eggs is 27-29 DEG C, phase
To humidity 70-80% and photoperiod 16L；And the bee syrup that addition concentration is 20-30% into oviposition box daily, and with the week of 1-3d
Collection of the phase to ovum is carried out in oviposition box；
S3, the man-made feeds prepared in S1 are placed in the first raising box, then the ovum collected in S2 is uniformly sprinkling upon the first raising
In box, and the first raising box is placed in environmental condition environment identical with oviposition box；
S4, after cacac moth egg hatching is at larva by the cacac moth larva of certain amount from first raising box be transferred to second
Box is raised, and 1 pairs of generation microbracon hebetor is introduced into the second raising box at bee；Into the second raising box, addition concentration is daily
The bee syrup of 10-70%；And the second raising box is placed in environmental condition environment identical with oviposition box；Deng 1 generation microbracon hebetor
Cacac moth larva is carried out 2 generation microbracon hebetors can be obtained after parasitic oviposition；
S5, bee pupa is shifted from the second raising box via a length of larva of oviparity and after tying pupa after 2 generation microbracon hebetor ovum in S4
Entrucking is carried out into packing box, and guarantees that the temperature in transportation environment locating for packing box is 12-18 DEG C.
2. a kind of artificial batch feeding packaging microbracon hebetor living body according to claim 1 exists at the method for bee, feature
In: the logarithm ratio 10:1 of the number of cacac moth larva and pairs of microbracon hebetor in the step S4.
3. a kind of method of the artificial batch feeding packaging microbracon hebetor living body according to claim 1 or 2 at bee, feature
Be: it is 10cm that the size of the second raising box, which is basal diameter, the cylinder box body structure of a height of 8cm.
4. a kind of artificial batch feeding packaging microbracon hebetor living body according to claim 1 exists at the method for bee, feature
In: the second raising box used in the step S4 includes tank body (1A)；Tank body (1A) is the column of both ends open inner hollow
Shape structure；And it is removably installed top cover (2A) and bottom cover (3A) respectively at the both ends of tank body (1A)；It is opened on top cover (2A)
There are ventilation through-hole (21A) and location hole (22A), and is covered with separation net (6A) in ventilation through-hole (21A)；Nutrition liquid pipe (7A)
One end by location hole (22A) be inserted into the inner cavity tank body (1A)；Cotton mass (8A) is fixed in the lower bottom part of nutrition liquid pipe (7A)；
Bottom cover (3A) includes the mounting disc (31A) being connected with tank body (1A)；It is opposite with the inner cavity of tank body (1A) on mounting disc (31A)
End face is provided with connector (32A)；Catch tray (33A) is installed on the other end of connector (32A)；Catch tray (33A)
Size and the inner wall size of tank body (1A) match.
5. a kind of artificial batch feeding packaging microbracon hebetor living body according to claim 4 exists at the method for bee, feature
In: external thread section (4A) is had at the both ends of tank body (1A), is provided with and the outer spiral shell on top cover (2A) and bottom cover (3A)
The internal thread segment (5A) that line section matches；Mounting disc (31A) on bottom cover (3A) is the integral structure with ring wall and disk；It is interior
Thread segment (5A) is arranged on the ring wall；The rod-like structure at the middle part of the disk is arranged in the position connector (32A)；Catch tray
Mounting groove (331A) is provided on the side wall of (33A)；And sealing ring (34A) is installed on the mounting groove (331A)；It is collecting
The upper surface of disk (33A) is removably installed once mild-natured gasket (332A).
6. a kind of artificial batch feeding packaging microbracon hebetor living body according to claim 1 exists at the method for bee, feature
In: the packing box used in the step S5；External packing box (1B) including inner hollow is removable in external packing box (1B)
It is equipped with unloading active box (2B), and has the first air inlet on two opposite side end faces of external packing box (1B)
(11B) and the first venthole (12B)；The outlet side that first air inlet (12B) pumps (3B) by pipeline with external cold air is connected；
Be provided on the side end face of active box (2B) second opposite with the first air inlet (11B) and the first venthole position (12B) into
Stomata (21B) and the second venthole (22B)；Medium position in active box (2B) is equipped with partition (4B)；And active box
Body (2B) is partitioned into several cells (23B) by the partition (4B)；And the inner wall of each cell (23B) is both provided with and determines
Position block (5B)；Storage bee tank (6B) is detachably disposed in active box (2B) by the locating piece (5B).
7. a kind of artificial batch feeding packaging microbracon hebetor living body according to claim 6 exists at the method for bee, feature
In: the first air inlet (11B) is arranged at the adjacent position in two side end faces of external packing box (1B)；On active box (2B)
Second air inlet (21B) is the different aperture of multiple sizes for being distributed on its side end face；And from close to far from the first air inlet
The position in hole (12B), the size of the aperture are in the trend being gradually increased；First venthole (12B) and the second venthole (22B)
It is all disposed within the side far from the first air inlet (11B).
8. a kind of artificial batch feeding packaging microbracon hebetor living body according to claim 6 exists at the method for bee, feature
In: it is provided on the inner bottom surface of external packing box (1B) locating slot (13B)；It is provided with and institute in the bottom surface of active box (2B)
State the inserted block (24B) that locating slot (12B) matches；Between the interior bottom of external packing box (1B) and active box (2B) outer bottom
It is also equipped with cushion pad (7B)；Storing up bee tank (6B) is cylindrical structure；Locating piece (5B) is in four ten in cell (23B)
The symmetrically placed column block structure of word；And the side that locating piece (5B) connects with storage bee tank (6B) is arcuate structure.
9. a kind of artificial batch feeding packaging microbracon hebetor living body according to claim 1 exists at the method for bee, feature
In: the oviposition box used in the step S2 includes the bottle cap (2) of bottle body (1) and setting on bottle body (1), described
The upper surface of bottle cap (2) offers air hole (3), and the first gauze (4) for sealing is equipped in the air hole (3),
The bottle body (1) is provided at being located at below bottleneck into insect population (5), it is described enter insect population (5) at sealed with tampon (6), described
The bottom of bottle body (1) offers circular hole, the second gauze (7) for sealing is equipped in the circular hole, under the bottle body (1)
Side is provided with collection ovum disk (8).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910489390.6A CN110235859B (en) | 2019-06-06 | 2019-06-06 | Method for artificially breeding packaged live moth cocoon bee pupas in batches |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910489390.6A CN110235859B (en) | 2019-06-06 | 2019-06-06 | Method for artificially breeding packaged live moth cocoon bee pupas in batches |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110235859A true CN110235859A (en) | 2019-09-17 |
| CN110235859B CN110235859B (en) | 2022-02-18 |
Family
ID=67886334
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910489390.6A Active CN110235859B (en) | 2019-06-06 | 2019-06-06 | Method for artificially breeding packaged live moth cocoon bee pupas in batches |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110235859B (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5133289A (en) * | 1991-09-05 | 1992-07-28 | Cornell Research Foundation, Inc. | Artificial system and method for breeding fleas |
| JPH07255323A (en) * | 1994-03-18 | 1995-10-09 | Kubota Corp | Method for breeding natural enemy insect |
| CN202799955U (en) * | 2012-09-24 | 2013-03-20 | 云南省烟草公司玉溪市公司 | Braconid container |
| CN208217309U (en) * | 2018-04-16 | 2018-12-11 | 长江大学 | A kind of roundheaded borer long-distance transport damping storage apparatus |
| CN208434568U (en) * | 2017-05-17 | 2019-01-29 | 贵州省烟草公司毕节市公司 | A kind of Novel tobacco grass meal snout moth's larva oviposition collection device |
| CN208639370U (en) * | 2018-08-22 | 2019-03-26 | 河南省农业科学院烟草研究所 | A kind of aphid parasite deadlock aphid release box |
-
2019
- 2019-06-06 CN CN201910489390.6A patent/CN110235859B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5133289A (en) * | 1991-09-05 | 1992-07-28 | Cornell Research Foundation, Inc. | Artificial system and method for breeding fleas |
| USRE35348E (en) * | 1991-09-05 | 1996-10-08 | Cornell Research Foundation, Inc. | Artificial system and method for breeding hematophagous insects |
| JPH07255323A (en) * | 1994-03-18 | 1995-10-09 | Kubota Corp | Method for breeding natural enemy insect |
| CN202799955U (en) * | 2012-09-24 | 2013-03-20 | 云南省烟草公司玉溪市公司 | Braconid container |
| CN208434568U (en) * | 2017-05-17 | 2019-01-29 | 贵州省烟草公司毕节市公司 | A kind of Novel tobacco grass meal snout moth's larva oviposition collection device |
| CN208217309U (en) * | 2018-04-16 | 2018-12-11 | 长江大学 | A kind of roundheaded borer long-distance transport damping storage apparatus |
| CN208639370U (en) * | 2018-08-22 | 2019-03-26 | 河南省农业科学院烟草研究所 | A kind of aphid parasite deadlock aphid release box |
Non-Patent Citations (3)
| Title |
|---|
| 奚家勤，韦建玉等: "《贮烟害虫种类及防治》", 31 January 2015 * |
| 袁敏，欧后丁等: "烟草粉螟对5种杀虫剂的敏感性测定", 《山地农业生物学报》 * |
| 陈浩梁: "麦蛾柔茧蜂规模化人工饲养的关键技术及其冷胁迫的反应机制", 《中国博士学位论文全文数据库农业科技辑》 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110235859B (en) | 2022-02-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105379683B (en) | A kind of gelechiid rearing method | |
| CN110604102B (en) | Chilo suppressalis standardized feeding and management method | |
| CN103238569A (en) | Device and method for raising predacious mites | |
| CN108077191A (en) | A kind of Spodoptera litura larvae group breeding method | |
| CN110150233B (en) | Method for killing stored tobacco meal moth by raising adult braconid flies of wheat moth braconid flies in batch | |
| CN110235858A (en) | A method of Storage tobacco cacac moth is killed so that cacac moth is host's feeding transport microbracon hebetor bee pupa | |
| CN108041353A (en) | A kind of dichocrocis punctiferalis man-made feeds and its preparation method and application | |
| CN108813114B (en) | Artificial feed for corn borer larvae as well as preparation method and application thereof | |
| CN117063780B (en) | Cordyceps sinensis ascospore harvesting culture medium and harvesting method thereof | |
| Cohen | Food consumption, food utilization, and metabolic rates of Geocoris punctipes [Het.: Lygaeidae] fed Heliothis virescens [Lep.: Noctuidae] eggs | |
| CN207355280U (en) | A kind of breeding apparatus of indoor feeding planthopper | |
| CN110367203A (en) | A kind of artificial batch feeding packaging microbracon hebetor living body at bee method | |
| CN110235859A (en) | A kind of method of artificial batch feeding packaging microbracon hebetor living body bee pupa | |
| CN109907007A (en) | A kind of device and method of large-scale breeding cacac moth | |
| CN110235857B (en) | Method for killing tobacco leaf pink borers in storage by feeding and transporting adult meadowworms with tobacco pink borers as hosts | |
| CN209234704U (en) | One kind being suitable for aleyrodid class or parasitic wasp class insects hatch, feeding device | |
| CN103704178A (en) | Method for adjusting diapause of Exorista civilis Rond | |
| CN112715490B (en) | Culicoides culture method and application thereof | |
| CN110150232A (en) | A kind of method that batch raising microbracon hebetor bee pupa kills storage cacac moth | |
| CN107853253B (en) | Indoor circulating breeding device and method for macrobrachium giganteum | |
| CN109247305B (en) | Tobacco nail feeding device and feeding method using same | |
| CN204362772U (en) | Speck flat angle stratiomyiid worm's ovum collector | |
| CN109588377B (en) | Silkworm egg hatching method | |
| CN109769757A (en) | The kind winged type insect breeding apparatus of one kind and method for breeding | |
| CN109247306B (en) | Tobacco beetle feeding and separating device and male and female adult separation method using same |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB03 | Change of inventor or designer information |
Inventor after: Jin Xin Inventor after: Yang Maofa Inventor after: Huang Ning Inventor after: Diao Chaoqiang Inventor after: Liao Yong Inventor after: Ou Houding Inventor after: Yang Hong Inventor after: Sun Guangjun Inventor after: Yang Xianbin Inventor before: Jin Xin Inventor before: Yang Maofa Inventor before: Diao Chaoqiang Inventor before: Liao Yong Inventor before: Ou Houding Inventor before: Yang Hong |
|
| CB03 | Change of inventor or designer information | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |