CN110235762A - Improve the hardening off method of uncaria tissue culture survival rate - Google Patents
Improve the hardening off method of uncaria tissue culture survival rate Download PDFInfo
- Publication number
- CN110235762A CN110235762A CN201910719635.XA CN201910719635A CN110235762A CN 110235762 A CN110235762 A CN 110235762A CN 201910719635 A CN201910719635 A CN 201910719635A CN 110235762 A CN110235762 A CN 110235762A
- Authority
- CN
- China
- Prior art keywords
- days
- hardening
- field planting
- uncaria
- management
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
- A01G24/25—Dry fruit hulls or husks, e.g. chaff or coir
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
- A01G24/27—Pulp, e.g. bagasse
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Soil Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Cultivation Of Plants (AREA)
Abstract
The present invention relates to a kind of method for training tissue culture seedling of Chinese medicine, belong to Plant tissue culture seedling hardening and Chinese medicine field of planting, in particular to the method for training tissue culture seedling of uncaria, this method is implemented in greenhouse, matrix selects coco bran, peat soil, perlite to be laid in seedbed after evenly mixing with certain proportion, and this method is related to soil humidity management, air humidity management, miniclimate temperature management and Nutrition Management etc..The matrix that hardening off method of the invention uses is at low cost, and hardening survival rate is up to 89.1% or more.
Description
Technical field
The present invention relates to a kind of method for training tissue culture seedling of Chinese medicine, belong to Plant tissue culture seedling hardening and Chinese medicine plantation neck
Domain, the in particular to method for training tissue culture seedling of uncaria.
Background technique
Uncaria is that Rubiaceae wild gambier belongs to perennial bejuco, is used as medicine with buckle stem branch, there is the arresting convulsion that relieves dizziness, high fever, infantile convulsions, epilepsy, etc., heat-clearing
The effect of calming the liver, is mainly used for liver wind agitation, twitch, hyperpyretic convulsion, children's crying with fear, pre-eclampsia, the diseases such as headache dizziness.
Modern medicine studies it and includes rhynchophyllin, there is the effect of blood pressure lowering, anti-platelet aggregation and antithrombotic, is used for treatment part heart and brain
Vascular diseases.At present using uncaria as the Chinese materia medica preparation of bulk pharmaceutical chemicals about more than 170, medicinal material annual requirement is big.It is wild to be distributed mainly on
The mountain valley of 1300 ~ 1900m of height above sea level is born in the ground such as Yunnan, Guangdong, Guangxi, Guizhou, Fujian, Hunan, Hubei, Jiangxi, the Yunnan place of production
In the sparse woods or bushes on small stream side.Guizhou, Guangxi have taken on a certain scale plantation at present.
In recent years, biotechnology carries out Chinese medicine in particular with tissue culture technology in the field of Chinese medicines using more and more extensive
The production of material seedling, it is advantageous that one side tissue culture technology can overcome part kind Traditional breeding processes to be only capable of breeding on a small quantity very
Extremely irreproducible bottleneck, such as bletilla, dendrobium nobile, hymsleya amabilis can be most on the other hand since tissue culture technology belongs to vegetative propagation field
The holding female parent merit of big degree can provide in a short time a large amount of seedlings, while tissue culture for the fine quality breeding of Chinese medicine
Technology, is a kind of sterile production technology, that is, the seedling obtained hardly band pathogen can also be obtained completely not by detoxification treatment
Seedling with any pathogen.But for most of Chinese medicine, the research of tissue culture technology is a group technology of skin seedling production
Difficult point, and the research of hardening technology is another technological difficulties that tissue-cultured seedling promotes and applies, and the current tissue culture technology of uncaria is more
Maturation, but the hardening survival rate of tissue-cultured seedling is lower.
Summary of the invention
The present invention disadvantage not high for above-described tissue-cultured seedling advantage and training tissue culture seedling survival rate, has invented one
Kind simple, low in cost, high survival rate the uncaria training tissue culture seedling technology of method.
The hardening off method of raising uncaria tissue culture survival rate of the invention, it is characterised in that following steps and method:
1) hardening canopy prepares: building the greenhouse of tool skylight and side window, the sunshade net of built-in 75 ~ 85% shading rate configures simultaneously
Seedbed and rainer.
2) matrix: coco bran, peat soil, perlite are laid in seedbed after evenly mixing with certain proportion.
3) hardening management:
A. soil humidity management: hardening started in 40 days, and soil humidity controls the soil humidity after 60% ~ 70%, hardening 40 days
It is maintained at 70% ~ 80%.
B. air humidity management: initial stage air humidity remains to 90 ~ 100% after field planting, and mid-term remains to 70 ~ 90%, the later period
Remain to 60 ~ 70%, it is finally synchronous with external environment.
C. miniclimate temperature management: after field planting in 40 days, miniclimate temperature is controlled at 5 ~ 28 DEG C, cannot be lower than 5 DEG C for a long time
Or it is higher than 28 DEG C.
D. Nutrition Management: after field planting, different times carry out pouring root with the water soluble composite fertilizer of various concentration.
Specifically, substrate composition ratio described in step 2 is coco bran: peat soil: perlite is 7 ~ 10:1 ~ 2:1 ~ 2.
Specifically, air humidity management method described in step 3) is that should carry out once spraying to guarantee air after being colonized
Humidity, after Small plastic shed is built on seedbed, after field planting in 10 days, it is spraying primary to take off Small plastic shed film every three days；10 ~ 20 after field planting
It, takes off 20 cm height of film daytime and breathes freely, close film at night；20 ~ 30 days after field planting, it is ventilative to take off film half daytime, while clear daily
Morning is spraying primary, closes film at night；30 ~ 40 days after field planting, takes off film daytime completely, close film at night；After field planting 40 days, film is taken off completely.
Specifically, miniclimate management method described in step 3) are as follows: when the temperature is excessively high, greenhouse roof window, side window can be carried out open
And the short time takes off Small plastic shed film, carries out closing roof window, side window when temperature is too low.
Specifically, Nutrition Management method described in step 3) is field planting 10 days ~ 40 days, every 7 ~ 10 days with 0.1% concentration water
Dissolubility compound fertilizer (20:20:20) pouring root is primary；After field planting 40 days, every 7 ~ 10 days water soluble composite fertilizers with 0.3% concentration
(20:20:20) pouring root is primary；After field planting 120 days, every 10 ~ 15 days water soluble composite fertilizers (20:20:20) with 0.5% concentration
Pouring root is primary.
Specifically, step 3) further includes illumination management, specifically: after field planting 90 days, at 18 points in 8 points ~ afternoon every morning,
Sunshade net is covered, remaining period closes sunshade net and uncaria seedling is exposed under sunlight；After field planting 120 days, 10 points of every morning ~ under
16 points of noon, sunshade net is covered, remaining period closes sunshade net and uncaria seedling is exposed under sunlight, after field planting 180 days, completely closes
Sunshade net.
Specifically, step 3) further includes disease crop smothering management, specifically: during hardening, greenhouse it is outer every 2 months with glyphosate
The outer control of weeds of canopy is carried out, while weeds between in removing seedbed and seedbed at any time；After field planting in 45 days, every 15 days with more than 50%
Bacterium 1500 times of liquid of spirit spray prevention and treatment；After field planting 45 days, prevention and treatment was sprayed with 50% carbendazim, 1000 times of liquid every 60 days.
It is coco bran, peat and perlite the beneficial effects of the present invention are: the matrix that hardening off method of the invention uses, and
Coco bran accounting is larger, and use cost is the half that traditional hardening mainly uses peat soil cost, and using the hardening of the matrix
Survival rate is 2 ~ 5 times using other formulation media hardenings such as peat soils up to 89.1% or more；Using hardening off method of the invention
After hardening 250 days, uncaria seedling can grow to 1.5 meters or more height, and the wooden rate of trunk is up to 40%, be equivalent to and cultivate 2 years
Seed seedling.
On the other hand, soil humidity management is an important factor for uncaria tissue-cultured seedling survives during hardening, traditional hardening base
Matter humidity is routinely 70% ~ 80%, and uncaria tissue-cultured seedling hardening early period process is lower using above-mentioned humidity survival rate, therefore this method
It controls using hardening soil humidity early period 60% ~ 70%, hardening survival rate improves about 30%.
Specific embodiment
Below with reference to example, the present invention is described further.Embodiments of the present invention are including but not limited to following
Embodiment.
Embodiment 1: in Nujiang Autonomous Prefecture of Yunnan Province Lushui city upper Changjiang region town, the implementation time is in October, 2018 for the present embodiment addressing.
1. hardening canopy prepares: the multispan greenhouse of tool skylight and side window is built, canopy is 2.5 meters high, and built-in mobilizable 85%
The sunshade net of shading rate, while configuring seedbed and rainer.
2. matrix prepares: being laid in seedbed, be laid with after coco bran, peat soil, perlite are mixed with the ratio uniform of 7:1:1
15 centimetres of thickness, Yu Dingzhi was sprinkled profoundly water before 3 days.
3. tissue-cultured seedling is colonized: will have the uncaria tissue-cultured seedling of 2 or more 1 centimetre or so length root systems, by 10*5 centimetres
Density is colonized, and is opened aperture with finger when field planting, uncaria tissue-cultured seedling root system is placed in hole, earthing to basal part of stem, and be compacted,
Seedling is set to stand on Medium Culture.It should be noted that when field planting and root system kept to unfold.
4. hardening management:
(1) soil humidity management: hardening started in 40 days, and soil humidity controls after 60% ~ 70%, hardening 40 days, and matrix is wet
Degree is maintained at 70% ~ 80%, should extend spray time when soil humidity is too small or increase spraying frequency, soil humidity is spent the time of tide, answered
Short time multifrequency time takes off film and reduces spraying frequency and time control water.
(2) air humidity management: after field planting, should carry out once it is spraying to guarantee air humidity, after arch is built on seedbed
High 40 centimetres of Small plastic shed；In field planting 10 days, take off that Small plastic shed film is spraying primary, and spray time should be according to base in seedbed every three days
The determination of matter humidity, when matrix is more dry, spray time can extend, and it is wet periodically to take off film control compared with the time of tide for matrix, while being often sprayed one every other day
It is secondary；It 10 ~ 20 days after field planting, takes off 20 cm height of film daytime and breathes freely, close film at night, when soil humidity is more dry, can carry out in due course
By spraying to supplement water supply in media；20 ~ 30 days after field planting, it is ventilative to take off film 1/3 daytime, while early morning is spraying primary daily, closes at night
Film；It 30 ~ 40 days after field planting, takes off film daytime completely, closes film at night, when soil humidity is more dry, can carry out in due course spraying with supplement
Water supply in media；After field planting 40 days, film is taken off completely.
(3) miniclimate temperature management: hardening initial stage, whole day open side window, only open skylight daytime, and 8 points to next day 8 at dusk
Point closes skylight, and miniclimate temperature is controlled at 15 ~ 28 DEG C, happened without being higher than 28 DEG C.In December, 2018 to 2 months 2019,
Only fine day opens skylight and side window, remaining non-fine day is turned off skylight and side window to keep the temperature, after in March, 2019, whole day opening side
Window and skylight.
(4) Nutrition Management: after field planting 10 days, every 7 days with 0.1% concentration water soluble composite fertilizer (20:20:20) pouring root one
It is secondary, mu pouring root amount double centner；After field planting 40 days, every 7 days water soluble composite fertilizer (20:20:20) pouring roots one with 0.3% concentration
It is secondary, mu pouring root amount double centner；After field planting 120 days, every 15 days water soluble composite fertilizer (20:20:20) pouring roots with 0.5% concentration
Once, 200 kilograms of mu pouring root amount.
(5) illumination management: after field planting 90 days, sunshade net is covered at 18 points in 8 points ~ afternoon every morning, remaining period, which closes, to be hidden
Uncaria seedling is exposed under sunlight by screened postive；After field planting 120 days, cover sunshade net at 16 points in 10 points ~ afternoon every morning, remaining when
Section closes sunshade net and uncaria seedling is exposed under sunlight, after field planting 180 days, completely closes sunshade net.
(6) sick crop smothering management: during hardening, greenhouse is outer to carry out control of weeds outside canopy with glyphosate every 2 months, while with
When remove seedbed in and seedbed between weeds；After field planting in 45 days, prevention and treatment was sprayed with 50% carbendazim, 1500 times of liquid every 15 days；It is fixed
After planting 45 days, prevention and treatment was sprayed with 50% carbendazim, 1000 times of liquid every 60 days.
5. transplanting: hardening 250 days, hardening survival rate was 91.6% at this time, 1.5 meters of seedling mean height, 1.8 meters of highest, minimum
0.9 meter, uncaria tissue-cultured seedling trunk whole lignifying, the wooden rate 50.6% of single plant trunk strikes off most of branches and leaves at this time, only stays
Trunk and 2 ~ 3 branch buds are transplanted, transplanting survival rate 98.2%.
As control group of the invention, in Nujiang Autonomous Prefecture of Yunnan Province Lushui city upper Changjiang region town, implementing the time is 2018 11 for addressing
Month, only change the matrix of above-described embodiment, after selecting peat soil (Pincee, 0 ~ 10), perlite to mix with the ratio uniform of 2:1
It being laid in seedbed, other experiment conditions are constant, and after hardening 250 days, hardening survival rate is only 67.9%, and 1.6 meters of seedling mean height, most
It is 1.9 meters high, minimum 1.1 meters, uncaria tissue-cultured seedling trunk whole lignifying, the wooden rate 50.5% of single plant trunk.
Embodiment 2: in the Nujiang Autonomous Prefecture of Yunnan Province Fugong County township Ma Ji, the implementation time is in April, 2018 for the present embodiment addressing.
1. hardening canopy prepares: building the multispan greenhouse of tool skylight and side window, canopy height is built-in movable higher than 2.5 meters
85% shading rate sunshade net, while configuring seedbed and rainer.
2. matrix prepares: being laid in seedbed, spread after coco bran, peat soil, perlite are mixed with the ratio uniform of 10:2:1
If 20 centimetres of thickness, Yu Dingzhi was sprinkled profoundly water before 2 days.
3. tissue-cultured seedling is colonized: will have the uncaria tissue-cultured seedling of 2 or more 1 centimetre or so length root systems, by 10*5 centimetres
Density is colonized, and is opened aperture with finger when field planting, uncaria tissue-cultured seedling root system is placed in hole, earthing to basal part of stem, and be compacted,
Seedling is set to stand on Medium Culture.It should be noted that when field planting and root system kept to unfold.
4. hardening management:
(1) soil humidity management: hardening started in 40 days, and soil humidity controls after 60% ~ 70%, hardening 40 days, and matrix is wet
Degree is maintained at 70% ~ 80%, should extend spray time when soil humidity is too small or increase spraying frequency, soil humidity is spent the time of tide, answered
Short time multifrequency time takes off film and reduces spraying frequency and time control water.
(2) air humidity management: after field planting, should carry out once it is spraying to guarantee air humidity, after arch is built on seedbed
High 30 ~ 50 centimetres of Small plastic shed；In field planting 10 days, take off that Small plastic shed film is spraying primary, and spray time should be according to seedbed every three days
Interior soil humidity determines that when matrix is more dry, spray time can extend, matrix compared with the time of tide, spray time be no more than 15s even not into
Row is spraying；10 ~ 20 days after field planting, takes off 20 cm height of film daytime and breathe freely, close film at night, when soil humidity is more dry, can carry out
It is in due course spraying to supplement water supply in media；20 ~ 30 days after field planting, it is ventilative to take off film 1/3 daytime, while early morning is spraying primary daily, evening
On close film；30 ~ 40 days after field planting, take off film daytime completely, close film at night, when soil humidity is more dry, can carry out it is in due course spraying with
Supplement water supply in media；After field planting 40 days, film is taken off completely.
(3) miniclimate temperature management: hardening initial stage, whole day open side window, only open skylight daytime, and 8 points to next day 8 at dusk
Point closes skylight, and miniclimate temperature is controlled substantially at 15 ~ 28 DEG C, and only the time is higher than 28 DEG C once in a while, and few time is higher than 30 DEG C.
After in late June, 2018, whole day opens side window and skylight.
(4) Nutrition Management: after field planting 10 days, every 10 days with 0.1% concentration water soluble composite fertilizer (20:20:20) pouring root one
It is secondary, 200 kilograms of mu pouring root amount；After field planting 40 days, every 10 days water soluble composite fertilizer (20:20:20) pouring roots with 0.3% concentration
Once, 200 kilograms of mu pouring root amount；After field planting 120 days, filled every 15 days water soluble composite fertilizers (20:20:20) with 0.5% concentration
Root is primary, 300 kilograms of mu pouring root amount.
(5) illumination management: after field planting 90 days, at 18 points in 8 points ~ afternoon every morning, covering sunshade net, remaining period is by uncaria
Seedling is exposed under sunlight；After field planting 120 days, at 16 points in 10 points ~ afternoon every morning, sunshade net is covered, remaining period is by uncaria seedling
It is exposed under sunlight, after field planting 180 days, removes sunshade net completely.
(6) sick crop smothering management: during hardening, greenhouse is outer to carry out control of weeds outside canopy with glyphosate every 1 month, answered simultaneously
Weeds between in removing seedbed and seedbed at any time；After field planting in 45 days, prevention and treatment was sprayed with 50% carbendazim, 1500 times of liquid every 10 days；
After field planting 45 days, prevention and treatment was sprayed with 50% carbendazim, 1000 times of liquid every 30 days.
5. transplanting: hardening 250 days, hardening survival rate was 89.1% at this time, 1.6 meters of seedling mean height, 2.1 meters of highest, minimum
1.1 meters, uncaria tissue-cultured seedling trunk whole lignifying, the wooden rate 59.5% of single plant trunk strikes off most of branches and leaves at this time, only stays
Trunk and 2 ~ 3 branch buds, transplanting survival rate 96.7%.
As control group of the invention, in the Nujiang Autonomous Prefecture of Yunnan Province Fugong County township Ma Ji, implementing the time is 2018 4 for addressing
Month, only change the matrix of above-described embodiment, selects rural area soil, sawdust to be laid in seedbed after the ratio uniform mixing of 5:1, other
Experiment condition is constant, and after hardening 250 days, hardening survival rate is only 32.6%, and 1.7 meters of seedling mean height, 2.3 meters of highest, minimum 1.4
Rice, uncaria tissue-cultured seedling trunk whole lignifying, the wooden rate 68.5% of single plant trunk strike off most of branches and leaves at this time, only stay trunk
And 2 ~ 3 branch buds, transplanting survival rate 88.6%.
Due to selecting rural area soil, there are a large amount of weed growths in seedbed during hardening, a large amount of manpower and material resources need to be spent to be removed
Grass, while in transplantation of seedlings out, since soil is just more hardened, emergence also needs to spend a large amount of manpowers and material resources.
Claims (7)
1. a kind of hardening off method for improving uncaria tissue culture survival rate, it is characterised in that following steps and method:
(1) hardening canopy prepares: building the greenhouse of tool skylight and side window, the sunshade net of built-in 75 ~ 85% shading rate is matched simultaneously
Set seedbed and rainer；
(2) matrix: coco bran, peat soil, perlite are laid in seedbed after evenly mixing with certain proportion；
(3) hardening management:
A. soil humidity management: hardening started in 40 days, and soil humidity controls the soil humidity after 60% ~ 70%, hardening 40 days
It is maintained at 70% ~ 80%；
B. air humidity management: initial stage air humidity remains to 90 ~ 100% after field planting, and mid-term remains to 70 ~ 90%, and the later period keeps
It is finally synchronous with external environment to 60 ~ 70%；
C. miniclimate temperature management: after field planting in 40 days, miniclimate temperature is controlled at 5 ~ 28 DEG C, cannot be for a long time lower than 5 DEG C or high
In 28 DEG C；
D. Nutrition Management: after field planting, different times carry out pouring root with the water soluble composite fertilizer of various concentration.
2. a kind of hardening off method for improving uncaria tissue culture survival rate according to claim 1, it is characterised in that step (2) institute
The substrate composition ratio stated is coco bran: peat soil: perlite is 7 ~ 10:1 ~ 2:1 ~ 2.
3. a kind of hardening off method for improving uncaria tissue culture survival rate according to claim 1, it is characterised in that step (3) institute
After the air humidity management method stated is field planting, should carry out once it is spraying to guarantee air humidity, after small arch is built on seedbed
After field planting in 10 days, it is spraying primary to take off Small plastic shed film every three days for canopy；10 ~ 20 days after field planting, it is saturating to take off 20 cm height of film daytime
Gas closes film at night；20 ~ 30 days after field planting, it is ventilative to take off film half daytime, while early morning is spraying primary daily, closes film at night；Field planting
It 30 ~ 40 days afterwards, takes off film daytime completely, closes film at night；After field planting 40 days, film is taken off completely.
4. a kind of hardening off method for improving uncaria tissue culture survival rate according to claim 1, it is characterised in that step (3) institute
The miniclimate management method stated are as follows: when the temperature is excessively high, can carry out open greenhouse roof window, side window and short time takes off Small plastic shed film, temperature
It carries out closing roof window, side window when spending low.
5. a kind of hardening off method for improving uncaria tissue culture survival rate according to claim 1, it is characterised in that step (3) institute
The Nutrition Management method stated is field planting 10 days ~ 40 days, is filled every 7 ~ 10 days with 0.1% concentration water soluble composite fertilizer (20:20:20)
Root is primary；It is primary every 7 ~ 10 days water soluble composite fertilizer (20:20:20) pouring roots with 0.3% concentration after field planting 40 days；Field planting
It is primary every 10 ~ 15 days water soluble composite fertilizer (20:20:20) pouring roots with 0.5% concentration after 120 days.
6. a kind of hardening off method for improving uncaria tissue culture survival rate according to claim 1, it is characterised in that step 3) is also
Including illumination management, specifically: after field planting 90 days, at 18 points in 8 points ~ afternoon every morning, sunshade net is covered, remaining period closes
Uncaria seedling is exposed under sunlight by sunshade net；After field planting 120 days, sunshade net is covered at 16 points in 10 points ~ afternoon every morning, remaining
Period closes sunshade net and uncaria seedling is exposed under sunlight, after field planting 180 days, completely closes sunshade net.
7. a kind of hardening off method for improving uncaria tissue culture survival rate according to claim 1, it is characterised in that step 3) is also
Including sick crop smothering management, specifically: during hardening, greenhouse is outer to carry out control of weeds outside canopy with glyphosate every 2 months, while with
When remove seedbed in and seedbed between weeds；After field planting in 45 days, prevention and treatment was sprayed with 50% carbendazim, 1500 times of liquid every 15 days；It is fixed
After planting 45 days, prevention and treatment was sprayed with 50% carbendazim, 1000 times of liquid every 60 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910719635.XA CN110235762A (en) | 2019-08-06 | 2019-08-06 | Improve the hardening off method of uncaria tissue culture survival rate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910719635.XA CN110235762A (en) | 2019-08-06 | 2019-08-06 | Improve the hardening off method of uncaria tissue culture survival rate |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110235762A true CN110235762A (en) | 2019-09-17 |
Family
ID=67893940
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910719635.XA Pending CN110235762A (en) | 2019-08-06 | 2019-08-06 | Improve the hardening off method of uncaria tissue culture survival rate |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110235762A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113141888A (en) * | 2020-01-07 | 2021-07-23 | 云南罗瑞生物科技有限公司 | Quick cutting propagation and seedling raising technology for medicinal vine tripterygium wilfordii twigs |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100620799B1 (en) * | 2005-05-06 | 2006-09-06 | 화이젠 주식회사 | In vitro regeneration and acclimatization of oleaceae plant |
| CN101578956A (en) * | 2009-06-22 | 2009-11-18 | 贵州信邦中药发展有限公司 | Standardization planting method of uncaria |
| CN102204503A (en) * | 2011-04-11 | 2011-10-05 | 中国热带农业科学院椰子研究所 | Modified coconut tree branny culture substrate and preparing method thereof |
| CN102934585A (en) * | 2012-12-04 | 2013-02-20 | 中国热带农业科学院香料饮料研究所 | Coffee cutting seedling culture method |
| CN102986510A (en) * | 2012-10-10 | 2013-03-27 | 肥西县农业技术推广中心 | Economical high-yield greenhouse Dendrobium officinale cultivation technique |
| CN108522216A (en) * | 2018-03-12 | 2018-09-14 | 农法自然（上海）农业科技有限公司 | Coconut palm chaff adds turf tissue-cultured seedling to practice seedling matrix improved method |
-
2019
- 2019-08-06 CN CN201910719635.XA patent/CN110235762A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100620799B1 (en) * | 2005-05-06 | 2006-09-06 | 화이젠 주식회사 | In vitro regeneration and acclimatization of oleaceae plant |
| CN101578956A (en) * | 2009-06-22 | 2009-11-18 | 贵州信邦中药发展有限公司 | Standardization planting method of uncaria |
| CN102204503A (en) * | 2011-04-11 | 2011-10-05 | 中国热带农业科学院椰子研究所 | Modified coconut tree branny culture substrate and preparing method thereof |
| CN102986510A (en) * | 2012-10-10 | 2013-03-27 | 肥西县农业技术推广中心 | Economical high-yield greenhouse Dendrobium officinale cultivation technique |
| CN102934585A (en) * | 2012-12-04 | 2013-02-20 | 中国热带农业科学院香料饮料研究所 | Coffee cutting seedling culture method |
| CN108522216A (en) * | 2018-03-12 | 2018-09-14 | 农法自然（上海）农业科技有限公司 | Coconut palm chaff adds turf tissue-cultured seedling to practice seedling matrix improved method |
Non-Patent Citations (4)
| Title |
|---|
| 施力军等: "钩藤组培技术体系研究", 《安徽农业科学》 * |
| 李春龙等: "《植物组织培养》", 30 July 2016, 西安交通大学出版社 * |
| 毛堂芬等: "钩藤的组织培养与植株再生", 《植物生理学通讯》 * |
| 高文远: "《中药生物工程》", 31 January 2014, 上海科学技术出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113141888A (en) * | 2020-01-07 | 2021-07-23 | 云南罗瑞生物科技有限公司 | Quick cutting propagation and seedling raising technology for medicinal vine tripterygium wilfordii twigs |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104620836B (en) | A kind of dendrobium candidum pseudo-wild cultivating method | |
| CN103098691B (en) | Dendrobium huoshanense pollution-free imitating wild planting process | |
| CN104126397B (en) | Bletilla striata direct sowing and seedling method | |
| CN102550252B (en) | Cultivation method of Dendrobium officinale | |
| CN104012271B (en) | A kind of method for growing dendrobium | |
| CN105638025B (en) | A method of rolling into a ball flower nursery | |
| CN104322257A (en) | Method for normatively planting dendrobium officinale in simple greenhouse | |
| CN104303797B (en) | A kind of greenhouse cultivation method of nuisanceless collard | |
| CN103688742A (en) | Floating seedling breeding method of erigeron breviscapus | |
| CN105993775A (en) | Culture method of Emmenopterys henryi | |
| CN102523902A (en) | High yield cultivation method for interplanting strawberries and melons | |
| CN105612988A (en) | Greenhouse planting method of dendrobium huoshanense | |
| CN106717829A (en) | The implantation methods of Panax Japonicus Var. Major | |
| CN102283064A (en) | Pollution-free cultivation method for dwarfed cherry | |
| CN107996333A (en) | A kind of David's-harp tissue culture method for transplanting | |
| CN107750787A (en) | A kind of implantation methods of granulated sugar tangerine | |
| CN107211700A (en) | A kind of nuisanceless watermelon breeding method | |
| CN106332762A (en) | Dendrobium planting manner | |
| CN106508347A (en) | Method for cultivation of angelica sinensis | |
| CN109874613A (en) | A kind of tor implantation methods of imitative dendrobium officinale | |
| CN108934679A (en) | A kind of root cutting growth of Ji Jing Aralia wood and seedling pipe support method | |
| CN1899019A (en) | Artificial breeding method for mitoridine wood | |
| CN108157052A (en) | The cultural method of blueberry tree | |
| CN107624467A (en) | A kind of fruit of a cubeb litsea tree method for culturing seedlings | |
| CN104303966B (en) | A kind of cultural method of wild gold lotus |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190917 |