CN104303966B - A kind of cultural method of wild gold lotus - Google Patents
A kind of cultural method of wild gold lotus Download PDFInfo
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- CN104303966B CN104303966B CN201410480707.7A CN201410480707A CN104303966B CN 104303966 B CN104303966 B CN 104303966B CN 201410480707 A CN201410480707 A CN 201410480707A CN 104303966 B CN104303966 B CN 104303966B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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Abstract
The invention discloses a kind of cultural method of wild gold lotus; including steps such as addressing, construction of base, seedling culture, seedling process and field management; by greenhouse automatic management, plantlet in vitro nurturing staff, before transplanting seedling Cotton seeds prevention and elimination of disease and pests harm and later stage rationally, timely field management; form standard set wild gold lotus cropping pattern; the wild gold lotus survival rate cultivated using the cropping pattern is high; being not easily susceptible to field adverse circumstances and pest and disease damage affects, and provides important evidence for scale, industrialization plantation wild gold lotus.
Description
Technical field
The present invention relates to a kind of plant cultivation technique, particularly a kind of cultural method of wild gold lotus.
Background technology
Roxburgh anoectochilus terminal bud, Classification system：Anoectochilus roxburghii(Wall.) Lindl,
English name：Shorthairy Antenoron, call Anoectochilus Roxburghii, orchid family, Anoectochilus Blume, herbaceous plant.Rich in kind more than 20
Vitamin and amino acid, have very strong inhibitory action to cancer cell, are exclusive a kind of very rare and famous and precious medicinal of China
Level ornamental plant.Roxburgh anoectochilus terminal bud happiness is shady and cool, moist, especially likes being grown in the limes marginis of subtropical evergreen broad-leaved, cliff, soil property pine
Scattered sough, it is desirable to 20 DEG C～32 DEG C of temperature, illumination are about the 1/3 of normal sunshine, most avoid direct sunlight.Due to its opposite
State environmental requirement is strict, and natural environment subject to severe risks of damage in recent years in addition, wild gold lotus are fewer and feweri, the market price drastically hurricane
Rise, up to 1000 yuan/kg, dried food and nuts is up to 10000 yuan/kg and out of stock in spite of rising price for current raw products procurement price.Roxburgh anoectochilus terminal bud is gone back in addition to medicinal
It is a kind of indoor sight leaf treasure of great ornamental value.Therefore, in order to protect and develop wild gold lotus this rare resources, closely
Over year, people are attempting planting wild gold lotus always, and the research with regard to roxburgh anoectochilus terminal bud artificial growth is also more, make a concrete analysis of as follows：
1st, 103766113 A of number of patent application CN disclose a kind of method that roxburgh anoectochilus terminal bud is planted in northern area, and it is main
Comprise the following steps：（1）Forest land selects；（2）Seedling is selected；（3）Seedbed prepares：Planting site selects northern temperature in spring, summer, autumn
Area between 15-30 DEG C of season, the soil under Chinese chestnut tree, it is desirable to which it is ventilative that the soil is porous, and moisture retention is good, furrow face matrix 1/2-2/
3 fertilizers mix mixed with 1/3-1/2 sylvan life humic fine earths；Before plantation, first carry out squirting sterilization with carbendazim or thiophanate,
Covered rearing with plastic film 2-3d is used again, then takes off film standby；（4）Transplant plantation；（5）Manage after plantation；（6）The steps such as results.
2nd, 102823408 B of patent authorization number CN discloses a kind of greenhouse soil planting method of anoectochilus formosanus, and concrete grammar is such as
Under：Bamboo greenhouse is built first and equipment of sprinkler irrigation is installed；Next selects peat soil for planting matrix, selection seedlings of anoectochilus preparation kind
Plant；Temperature, humidity during planting roxburgh anoectochilus terminal bud again in attention regulation bamboo greenhouse, luminosity, ventilation condition, beneficial to the life of roxburgh anoectochilus terminal bud
It is long.
3rd, 102792838 A of number of patent application CN disclose a kind of roxburgh anoectochilus terminal bud greenhouse cultivation method, including anoectochilus blume seedling is tamed and dociled
Change, cultivation matrix is sterilized, plant, temperature and illumination control this four steps；The culture cup for having planted anoectochilus blume seedling is put into into plastics
In bag, several apertures are opened up higher than the position of culture cup in polybag；Pour water into the bottom of polybag, then the bag to polybag
Mouth carries out encapsulation process, and polybag is suspended on below the seedbed in greenhouse, and after such scheme, moisture is steamed inside sack
Send out, not easily run off, the moist environment that a suitable roxburgh anoectochilus terminal bud is grown up is formed in polybag, such anoectochilus blume seedling would not be by sky
The pollution of the various bacteriums in gas or water and cause pest and disease damage, without using agricultural chemicals still ensure that anoectochilus blume seedling have it is higher into
Motility rate.
4th, number of patent application CN 102823412A disclose a kind of roxburgh anoectochilus terminal bud non-polluted planting method, including seedling tray
Selection, the screening of cultivation matrix, scientific management technique and pollution-free management method, can using present invention plantation roxburgh anoectochilus terminal bud
To effectively improve the quality and yield of roxburgh anoectochilus terminal bud.
The method that roxburgh anoectochilus terminal bud is planted under controllable environment is employed in four inventions of the above, but there is problems with：
1st, seedling is easily caused harm by field pest and disease damage in the process transplanted after tissue culture or alternate manner culture to field, with
Upper technology does not carry out long-term, effective Cotton seeds to seedlings of anoectochilus, and the later stage needs substantial amounts of energy controlling disease；2、
Although above technology cultivates roxburgh anoectochilus terminal bud in greenhouse or more closed environment, it is extraneous that easy " booth " facility is difficult to opposing
The weather such as force majeure, such as strong wind, heavy rain, heavy snow, causes to complete immediately roxburgh anoectochilus terminal bud from seedling to the process transplanted, but
Still it is difficult in terms of final-period management and large-area applications；3rd, simple " canopy body " facility is difficult to ensure that effective control of humiture
System, keeps out direct sunlight and artificially applying fertilizer dispenser by artificial spray cooling, sunshade net not tight enough in management, to gold thread
The cultivation of lotus can cause larger difficulty.
In addition, number of patent application CN 103283572A are bright to disclose a kind of aerosol cultivation method for anoectochilus roxburghii, seedlings of anoectochilus
After cleaned and sterilization, it is fixed in an aerosol culture apparatus, and its root is exposed；The aerosol culture apparatus is provided with a sealing
Space, by the roxburgh anoectochilus terminal bud fixing holes that are interlinked to the outside in a plurality of are set on wall, by roxburgh anoectochilus terminal bud leaves and stemses outwardly, root is towards fixed
On fixing hole；It is provided with sealing space by the sprayer of nutrient solution aerosolization, by exposed root absorption after nutrient solution aerosolization.
There is a higher theoretical using value relative to earth culture using the technology, but in large-scale industrialization, large-scale planting field also
Have it is many need solve problem, such as model of the aerosol culture apparatus in large-scale production, specification, be difficult to grasp using technology
Make.
Number of patent application CN 103563635A disclose a kind of roxburgh anoectochilus terminal bud implantation methods, if beating at the side interval of container
Dry aperture, the soil on container content are implanted into roxburgh anoectochilus terminal bud seedling in aperture, after water spray slow seedling, will be implanted with the container of roxburgh anoectochilus terminal bud
It is suspended on the shelf on the branch under the woods or under the woods, technology roxburgh anoectochilus terminal bud growth is changed to be suspended on the woods by underground and is sowed
Plant, insect can be avoided to cause harm and rain drop erosion, improve the survival rate of roxburgh anoectochilus terminal bud, the inventive technique cultivates gold with suspension arrangement
Line lotus as test or research, but can be difficult to accomplish scale production in actual environment in a small range.
Number of patent application CN 103340068A disclose a kind of roxburgh anoectochilus terminal bud cultivation under woods matrix and cultural method, and it selects
As roxburgh anoectochilus terminal bud cultivation place under the forest environment of suitable nature degree of shading and intensity of illumination；By drawing shallow on cultivation matrix
Cave, pours root water after field planting；Adjustment air humidity 75-90%, temperature 15-30 DEG C；Spray when cultivation matrix surface bleaches after plantation
Mist waters, and spraying every time is watered to cultivation matrix and irrigating, not only contributed to roxburgh anoectochilus terminal bud growth, can significantly improve roxburgh anoectochilus terminal bud
Yield, survival rate, Drying rate, and easy to operate, low production cost.The patented technology must be chosen to be at shelters from heat or light under forest
Roxburgh anoectochilus terminal bud can just be planted, for large-scale planting has more limiting factor, while under natural environment control humiture have compared with
More can not Manipulative Factors, it is also difficult to realize the cultivating and growing technology of standard.
As can be seen here, although the cultivating and growing technology of existing more roxburgh anoectochilus terminal bud is constantly studying, but still suffers from various standards
, insoluble problem in controllable extensive wild gold lotus cultivation.
The content of the invention
The purpose of the present invention is for the problems referred to above existing for prior art, there is provided a kind of cultivation of wild gold lotus
Technology, it is possible to achieve the manual scale planting of wild gold lotus, provides thinking to standardize wild gold lotus plantation.
The technology of the present invention content is realized in：
The cultural method of wild gold lotus, including addressing, construction of base, seedling culture, seedling process and field management 5
Step, specially：
1. addressing：Selection has a good transport service, and hydroelectric facility is complete, while the sunshine-duration is shorter, intensity of illumination is weaker, cool place is wet
As planting base where profit；Due to roxburgh anoectochilus terminal bud it is relatively low in necessary for growth temperature, while the wet property of happiness, in selection
Stating the place of requirement, to grow as planting base be necessary, therefore mountain side, woodland, lowland or pile are cool behind etc.
Refreshing, partially cloudy place becomes optimal selection.
2. construction of base：
A, build it is sun-shading rain-proof shed, with concrete column support whole shed body, bamboo chip, reinforcing bar or iron pipe are curved semilune, are pressed
Certain intervals are fixed into frame side by side and assign on the ground, and transparent agricultural film and sunshade net are covered on canopy face, you can；It is sun-shading rain-proof shed
Similar to vegetable greenhouse booth, not only can keep out wind and rain in canopy tumor growth and resist extraneous force majeure, ensure roxburgh anoectochilus terminal bud
Growing environment is not influenced by the adverse circumstances, meanwhile, in the greenhouse can at any time, accurately, fixed point, adjust roxburgh anoectochilus terminal bud growth on demand
The operation such as required water, fertilizer, medicine.
B, build ground, play furrow and ditch, carrying out building ground according to the width of canopy, after determining that furrow, the quantity of ditch and width, furrow ditch determine
Furrow are carried out.
Preferably, it is in the base of canopy width 4m, wide according to 3 railway carriage or compartment 80cm wide furrow and 4 40cm wide ditch or 4 railway carriage or compartment 60cm
Furrow and 5 wide ditch two ways of 32cm play furrow；Then the thick cinders of one layer of 10cm or thick river sand are spread at furrow bottom, repaves last layer
10cm thickness fully mixes the matrix that retting is become thoroughly decomposed thoroughly by dry cattle and sheep excrement 10%, rotted leaf 45% and wooden chaff 45%, the process of three's composition,
Finally the weedtree chaff that thick layer 1cm was soaked through 5 ‰ thiophanate methyls is covered again.
C, installation automatic spraying device, automatic fertilization, device for administration of drugs.
Preferably, multilayer built pattern of farming can also be set up in construction of base, is advisable every 60-70cm per interlayer；Build
The purpose of vertical multilayer built pattern of farming is to make full use of space advantage, and in rational planning growing area, wasting space is not excellent
Gesture, improves plantation efficiency.
3. seedling culture, cultivates plantlet in vitro, takes stem section that wild gold lotus contain a stipes as explant, sterilization
Afterwards, under aseptic processing environment, first cultivate on MS culture mediums, be then forwarded to proliferated culture medium cultivation.
The proliferation culture medium formula component is：MS culture medium 0.2-0.8mg/L+ a- methyl α-naphthyl acetate 0.2-0.8mg/L+
Indolebutyric acid 0.1-0.3mg/L+ nano-sized carbon 1.5-3.5g/L+ sucrose 33～43g/L+, 5.5～6.5g/L+ of agar potatos 2.5
～5.5g/L+ 1.5～3.5g/L of glucose.
4. seedling process, roxburgh anoectochilus terminal bud plantlet in vitro is soaked in nutrient solution 12-24 hour Cotton seeds；Clustered plants will be divided
For individual plant, individual plant is long will to cut out short, and with every plant of 2-3 leaf, length 4-6cm is advisable；By pretreated kind transplantation of seedlings to canopy
In matrix.
The nutrient solution recipe ingredient is：Copper sulphate 0.01-0.1g/L+ zinc sulfate 0.01-0.1g/L+ ammonium molybdate 0.01-
0.1g/L+ ferrous sulfate 0.01-0.1g/L+ manganese sulfate 0.01-0.1g/L+boric acid 0.01-0.1g/L+amino-oligosaccharide
+ 6000 0.2-1g/L white sugar 2.5～3.5g/L+ grape juices of 0.01-0.1g/L+ thiophanate methyl 0.2-0.5g/L+ polyethylene glycol
1.5～3.5g/L.
5. it is colonized and manages：
A, implantation time：Can all plant throughout the year, but be preferred to April next year with annual September.
B, planting depth are 2-3cm, and seeding row spacing 7-10cm is advisable, and the 5 ‰ thiophanate methyl liquid medicine that drench are sprayed after plant.
C, plant after to spray 1-2 time daily, to monthly spray sterilization drugs 1-2 time, to keep soil to moisten, prevent disease
Occur；After taking root, can monthly spray 5 ‰ composite fertilizer 1 time, or according to growing state per 1-2 month organic fertilizers once.
The fertilizer is the composting fertilizers such as cattle and sheep argol or weedtree chaff through high temperature retting.
D, the height rolled according to Changes in weather situation, adjustment the moon canopy both sides agricultural film and screened postive, it is appropriate in canopy to ensure
Ventilation and humiture.
The step 2. in transparent agricultural film and sunshade net can automatic deploying and retracting.
4. the measure of d items can also be solved the step by automatic temperature and humidity control system is installed in canopy.
Technical solution of the present invention has following marked improvement and advantage relative to traditional technology：
First, the cultural method of wild gold lotus of the invention, Jing inventor facts have proved more：The cultivation technique is complete
Can solve the problems, such as that wild gold lotus artificial growth is difficult, according to the technology in inventive technique scheme, wild gold lotus are artificial
Grow fine under planting environment, yield and quality have raising in various degree, and the cultivation technique in standardization, procedure kind
A set of plantation standard is formed during planting wild gold lotus, can be got through from harvesting, check, be worked into all of sale by standard
Link, the resource regeneration for wild gold lotus provide important evidence.
2nd, the cultural method of wild gold lotus of the invention, is carried out in greenhouse, and the greenhouse base set up according to the rules makes
With reinforcing bar or steel pipe as bracing frame, service life is changed and traditional uses bamboo as skeleton short life up to 15-20
Problem；Meanwhile, the infringement brought to canopy body because of exceedingly odious weather such as heavy snow, hails can be reduced using steel skeleton,
Once and for all.
3rd, the cultural method of wild gold lotus of the invention, has used the technical scheme of " plantlet in vitro+nutrient solution ", tissue culture
Seedling can reduce various pest and disease damages of the roxburgh anoectochilus terminal bud in seedling stage and cause harm, and for nurturing staff, tame, take root etc. and provide good ring
Border；And use nutrient solution to tissue culture transplantation of seedlings advance row Cotton seeds, form protective layer in root, the lasting period up to 1-5 month,
The direct infringement not by pest and disease damage in soil or in fertilizer is ensure that after transplanting, is reduced and is survived risk, improve safety
Coefficient.
4th, the cultural method of wild gold lotus of the invention, automation mechanized operation, specification safety.It is automatic in warm indoor location
Spray equipment, automatically spray, fertilizer apparatus, while adjusting humiture change by temperature automatically controlled, the wet equipment of control, allow roxburgh anoectochilus terminal bud to exist
The risk during cultivation management can be reduced completely under the environment of intelligence, survival rate is improved, reduce it is artificial put into, improve work
Efficiency.
5th, the cultural method of wild gold lotus of the invention, the plantlet in vitro proliferated culture medium for using, seedling process culture
In liquid, canopy, rationally, Jing experimental verifications repeatedly are raw after the seedling culture, transplanting for wild gold lotus for matrix and organic fertilizer formula
Length is optimum formula ratio, under orderly management pattern, is easier to the growth of management and control wild gold lotus, development, for open country
Raw roxburgh anoectochilus terminal bud realizes that the realization of extensive artificial growth provides significant data.
6th, the cultural method of wild gold lotus of the invention, Chinese southern and northern it is achieved that because passing through greenhouse bar
The Modulatory character of part, thus it is possible to vary the reality that wild gold lotus can only be grown up in southern area of China is southern and northern while introducing
Wild gold lotus provide help.
Specific embodiment：
Embodiment 1
The cultivation that inventor has carried out wild gold lotus in Qinzhou periphery in -2013 years 2011 is attempted, the ground
Block area is about 400m2, canopy width 4m, periphery traffic be flourishing, belongs to lowland landform, and the surrounding hilltop has Eucalyptus Stand to plant, all the year round
, at 22-28 DEG C, humidity is larger for temperature range, is wasteland in the past, belongs to laterite soil, phosphorous abundant.First, the plot is carried out
The construction of warmhouse booth：
A, with concrete column support whole shed body, cement column length be tri- kinds of specifications of 1m, 1.5m and 2m, respectively in canopy body outermost
Cement support is built in side-centre, and then semilune iron pipe supports canopy face as support, is fixed into canopy side by side by 2m spacing distances
Frame, support are built after finishing, and cover transparent agricultural film and fix, then sunshade net is placed on agricultural film on canopy face, fixed, i.e.,
Can.
B, furrow are played according to 3 railway carriage or compartment 80cm wide furrow and 4 40cm wide ditches, or can also be according to 4 40cm wide ditch or 4
Railway carriage or compartment 60cm wide furrow and 5 wide ditches of 32cm play furrow, and site preparation is tied, and is spread the thick cinders of one layer of 10cm at furrow bottom, is repaved last layer
10cm thickness fully mixes the matrix that retting is become thoroughly decomposed thoroughly by dry cattle and sheep excrement 10%, rotted leaf 45% and wooden chaff 45%, the process of three's composition,
Finally the weedtree chaff that thick layer 1cm was soaked through 5 ‰ thiophanate methyls is covered again.
Multiple automatic spraying devices are installed above every furrow, 5m is spaced, while medicine-chest and fertilizer are installed in spray equipment starting point
Material adds passage, installs agricultural film and sunshade net automatic control system in canopy body entrance, middle and innermost end.
C, multilayer built pattern of farming is set up, per interlayer every 60-70cm；The purpose for setting up multilayer built pattern of farming is
Space advantage is made full use of, in rational planning growing area, wasting space advantage, does not improve plantation efficiency.
3. seedling culture, cultivates plantlet in vitro, takes stem section that wild gold lotus contain a stipes as explant, sterilization
Afterwards, under aseptic processing environment, first cultivate on MS culture mediums, be then forwarded to proliferated culture medium cultivation, Multiplying culture basigamy
Fang Zufen is：MS culture medium 0.2-0.8mg/L+ a- methyl α-naphthyl acetate 0.2-0.8mg/L+ indolebutyric acid 0.1-0.3mg/L+ nanometers
Carbon 1.5-3.5g/L+ sucrose 33～43g/L+ agar 5.5～6.5g/L+ potato 2.5～5.5g/L+, 1.5～3.5g/ of glucose
L。
Roxburgh anoectochilus terminal bud plantlet in vitro, is soaked 12-24 hour Cotton seeds in nutrient solution at a by 4. seedling process, treat after process into
Root of having gone forms one layer of medicine film into next step process；B, clustered plants will be divided into individual plant, and individual plant is long will to cut out short, with per plant
2-3 leaf, length 4-6cm are advisable；C, by pretreated kind transplantation of seedlings to matrix in canopy；
Nutrient solution recipe ingredient is：Copper sulphate 0.01-0.1g/L+ zinc sulfate 0.01-0.1g/L+ ammonium molybdate 0.01-0.1g/
L+ ferrous sulfate 0.01-0.1g/L+ manganese sulfate 0.01-0.1g/L+boric acid 0.01-0.1g/L+amino-oligosaccharide 0.01-
0.1g/L+ thiophanate methyl 0.2-0.5g/L+ polyethylene glycol（6000）0.2-1g/L+ 2.5～3.5g/L+ of white sugar grape juices 1.5
～3.5g/L；
5. it is colonized and manages：
A, implantation time：Select to transplant plantation within 31st in September in 2011.
B, planting depth are 2-3cm, and seeding row spacing 7-10cm is advisable, and spray 5 ‰ first by automatic sprinkler fire-extinguishing system after plant
Base thiophanate liquid medicine.
C, plant after to spray daily 1 time, when spray time is morning 9-11, to monthly spray sterilization drugs 1-2 time, with guarantor
Soil moistening is held, prevents disease from occurring；After taking root, 5 ‰ composite fertilizer 1 time can be monthly sprayed, or according to growing state per applying the 1-2 months
The composting fertilizers such as the cattle and sheep argol or weedtree chaff through high temperature retting are once.
D, the height rolled according to Changes in weather situation, Reasonable adjustment the moon canopy both sides agricultural film and screened postive, to ensure to fit in canopy
When ventilation and humiture, concrete grammar is：
When intensity of sunshine is larger and during continuous sunny, puts down sunshade net and sprays 1 water.
When running into continuous drying rainfall and being few, spray 2 times daily.
When temperature is more than 30 DEG C, opens agricultural film and suitably divulge information and 1-2 times/day and spray 1 water.
When temperature is less than 18 DEG C, temperature regulator is opened, regulating and controlling temperature is more than 22 DEG C.
When running into continuous cloudy day humidity and being larger, humidity regulation device is opened, moisture in greenhouse is discharged, humidity is kept in 70-
85%。
6. pest management：
Wild gold lotus can run into following pest and disease damage in planting process：
Seedling stage（Cutworm, grub, wireworm；Samping off, damping-off）
Growth period（Red spider, aphid, trialeurodes vaporariorum；Virus）
Can rationally be prevented and treated using following medicament：
Commercially available agricultural chemicals is using medicament.
Embodiment 2：Survival rate and quality are effectively increased with the method for above-mentioned domestication wild roxburgh anoectochilus terminal bud：
Compared with conventional method domestication wild roxburgh anoectochilus terminal bud survival rate using above-mentioned cultural method
As can be seen that the planting type of the present invention can ensure that the survival rate up to more than 95% of roxburgh anoectochilus terminal bud, and traditional cultivation
Training pattern is unstable and survival rate is relatively low, and plantation for years experience have shown that：The cultural method of the wild gold lotus completely may be used
With the needs of roxburgh anoectochilus terminal bud of accomplishing scale production, detect through relevant department, the roxburgh anoectochilus terminal bud medicinal ingredient that Jing cultivations are bred reaches
To criterion of acceptability.
Claims (6)
1. a kind of cultural method of wild gold lotus, it is characterised in that：Including addressing, construction of base, seedling culture, seedling process
With 5 steps of field management, specially：
1. addressing：Selection has a good transport service, and hydroelectric facility is complete, while the sunshine-duration is shorter, intensity of illumination is weaker, shady and cool moistening
Place is used as planting base；
2. construction of base：
A, build it is sun-shading rain-proof shed, with concrete column support whole shed body, bamboo chip, reinforcing bar or iron pipe are curved semilune, by certain
Interval is fixed into frame side by side and assigns on the ground, and transparent agricultural film and sunshade net are covered on canopy face, you can；
B, build ground, play furrow and ditch, carrying out building ground according to the width of canopy, determine that furrow, the quantity of ditch and width, furrow ditch are carried out after determining
Play furrow；
C, installation automatic spraying device, automatic fertilization, device for administration of drugs；
3. seedling culture：Plantlet in vitro is cultivated, and stem section that wild gold lotus contain a stipes is taken as explant, after sterilization,
Under aseptic processing environment, first cultivate on MS culture mediums, be then forwarded to proliferated culture medium cultivation；
4. seedling process：Roxburgh anoectochilus terminal bud plantlet in vitro is soaked in nutrient solution 12-24 hour Cotton seeds；Clustered plants will be divided into list
Strain, individual plant is long will to cut out short, every plant of 2-3 leaf, length 4-6cm；By in pretreated kind transplantation of seedlings to matrix in canopy；
5. field management：
A, implantation time：Can all plant throughout the year；
B, planting depth are 2-3cm, seeding row spacing 7-10cm, and the 5 ‰ thiophanate methyl liquid medicine that drench are sprayed after plant；
C, plant after to spray 1-2 time daily, to monthly spray sterilization drugs 1-2 time, to keep soil to moisten, prevent disease
It is raw；After taking root, monthly 5 ‰ composite fertilizer of spray 1 time, or according to the every 1-2 month organic fertilizers of growing state once；
D, the height rolled according to Changes in weather situation, the sun-shading rain-proof shed both sides agricultural film of adjustment and sunshade net, to ensure to fit in canopy
When ventilation and humiture；
Step 3. described in proliferation culture medium formula component be：MS culture medium 0.2-0.8mg/L+a- methyl α-naphthyl acetate 0.2-0.8mg/L+
Indolebutyric acid 0.1-0.3mg/L+ nano-sized carbon 1.5-3.5g/L+ sucrose 33～43g/L+, 5.5～6.5g/L+ of agar potatos 2.5
～5.5g/L+ 1.5～3.5g/L of glucose；
Step 4. described in nutrient solution recipe ingredient be：Copper sulphate 0.01-0.1g/L+ zinc sulfate 0.01-0.1g/L+ ammonium molybdates
0.01-0.1g/L+ ferrous sulfate 0.01-0.1g/L+ manganese sulfate 0.01-0.1g/L+ boric acid 0.01-0.1g/L+ amino-oligosaccharides
0.01-0.1g/L+ thiophanate methyl 0.2-0.5g/L+ Macrogol 6000 0.2-1g/L+ white sugar 2.5～3.5g/L+ grape juices
1.5～3.5g/L.
2. the cultural method of wild gold lotus according to claim 1, it is characterised in that：Step 2. described in build ground, rise
The method of furrow and ditch is：It is in the base of canopy width 4m, wide according to 3 railway carriage or compartment 80cm wide furrow and 4 40cm wide ditch or 4 railway carriage or compartment 60cm
Furrow and 5 wide ditch two ways of 32cm play furrow；Then the thick cinders of one layer of 10cm or thick river sand are spread at furrow bottom, repaves last layer
10cm is thick by dry cattle and sheep excrement 10%, leaf mould 45% and wooden chaff 45%, and the process of three's composition fully mixes the matrix that retting is become thoroughly decomposed thoroughly,
Finally the weedtree chaff that thick layer 1cm was soaked through 5 ‰ thiophanate methyls is covered again.
3. the cultural method of wild gold lotus according to claim 1, it is characterised in that：Step 2. described in construction of base
Also include setting up multilayer built pattern of farming, per interlayer every 60-70cm.
4. the cultural method of wild gold lotus according to claim 1, it is characterised in that：Step 2. described in transparent agricultural film
Can automatic deploying and retracting with sunshade net.
5. the cultural method of wild gold lotus according to claim 1, it is characterised in that：Step 5. described in humiture
Control, is realized by automatic temperature and humidity control system is installed in canopy.
6. the cultural method of wild gold lotus according to claim 1, it is characterised in that：Step 5. described in fertilizer be
Through the cattle and sheep argol or weedtree chaff composting fertilizer of high temperature retting.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410480707.7A CN104303966B (en) | 2014-09-19 | 2014-09-19 | A kind of cultural method of wild gold lotus |
Applications Claiming Priority (1)
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108739324A (en) * | 2018-06-04 | 2018-11-06 | 安徽俊祺生物科技有限公司 | A kind of cultural method of roxburgh anoectochilus terminal bud |
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| CN106258444A (en) * | 2016-09-05 | 2017-01-04 | 深圳市双赢伟业科技股份有限公司 | Standardization sand base breeding method and Chinese crude drug standardization sand base breeding method |
| CN107711321A (en) * | 2017-10-11 | 2018-02-23 | 贺州市潇贺古道特色产业发展有限公司 | The method of the wild cultivation of roxburgh anoectochilus terminal bud |
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| CN102823408B (en) * | 2012-09-11 | 2014-01-08 | 泉州市金胜生态农业有限公司 | Greenhouse soil planting method of anoectochilus formosanus |
| CN102823412B (en) * | 2012-09-17 | 2015-08-05 | 地缘（厦门）生物科技有限公司 | Roxburgh anoectochilus terminal bud non-polluted planting method |
| CN102907327B (en) * | 2012-11-07 | 2013-09-04 | 厦门加晟生物科技有限公司 | Tissue culture propagation method for anoectochilus roxburghii |
| CN103340068B (en) * | 2013-06-06 | 2015-05-13 | 福建省农业科学院农业生物资源研究所 | Undergrowth cultivation medium and cultivation method for anoectochilus formosanus |
| CN103766113B (en) * | 2013-12-06 | 2015-06-24 | 福建省农业科学院甘蔗研究所 | Method for planting anoectochilus formosanus in northern area |
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| CN108739324A (en) * | 2018-06-04 | 2018-11-06 | 安徽俊祺生物科技有限公司 | A kind of cultural method of roxburgh anoectochilus terminal bud |
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