CN110229351A - 一种抗菌纤维素水凝胶的制备方法与应用 - Google Patents
一种抗菌纤维素水凝胶的制备方法与应用 Download PDFInfo
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Abstract
本发明公开了一种抗菌纤维素水凝胶的制备方法。首先配制抗菌剂溶液,将混合纤维素原料充分混合均匀,溶解于抗菌剂溶液中,制成纤维素溶液;在上述溶液中加入胶态二氧化硅CSPs稀释液,得到混合溶液;搅拌,于25~45℃下加热3~10min,经物理交联作用制成抗菌纤维素水凝胶。本发明抗菌纤维素水凝胶的制备工艺简单,纤维素衍生物通过动态和多价的选择性吸附作用连接至二氧化硅纳米颗粒上,同时加载抗菌剂药物,经物理交联作用即可成胶,适合于大规模工业生产。本发明制备的水凝胶能够应用于伤口辅料医疗卫生领域,对革兰氏阴性菌大肠杆菌和革兰氏阳性菌枯草芽孢杆菌具有抑菌作用。
Description
技术领域
本发明属于生物材料技术领域,具体涉及一种抗菌纤维素水凝胶的制备方法与应用。
背景技术
近年来,细菌等有害微生物在医疗领域中给人类带来极大的威胁。大量的抗生素被应用于治疗细菌感染性疾病,这导致了细菌对大部分抗生素药物产生了耐药性。目前延缓细菌耐药性主要有两种方法:一种是最大限度减少抗生素的使用,另一种是使用可以替代抗生素的新型抗菌剂。因此,开发新的抗菌材料已成为当前的研究热点。
根据制备水凝胶原料的来源不同,可以将其分为合成高分子水凝胶和天然高分子水凝胶。合成高分子水凝胶易于工业化生产合成、但存在难以降解、生物安全性低等缺点。天然高分子水凝胶具有可降解、生物安全性高、亲水性等特点,广泛应用于组织工程、生物材料和药物传递等领域。因此,选用天然高分子原料也成为一种趋势。
天然高分子纤维素水凝胶的生物安全性很高,对人体无毒副作用,适合用作与人体有亲密接触的医用材料,其为抗菌材料的开发提供了一个新的有用的起点。本发明将抗菌剂负载到凝胶网络中,它们一旦被水凝胶负载,可以在伤口附近局部使用,并且是减少用药剂量和抵抗耐药性的好方法。本发明制备的材料可广泛应用于生物医药领域,具有广阔的应用市场和极其高的产业附加值。
发明内容
发明目的:本发明目的在于提供一种抗菌纤维素水凝胶的制备方法。
技术方案:本发明的目的通过下述技术方案实现:
一种抗菌纤维素水凝胶的制备方法,包括以下步骤:
(1)配制浓度为200~1000μg/ml的抗菌剂溶液;
(2)将混合纤维素原料室温充分混合均匀后,溶解于所述抗菌剂溶液中,制成浓度2~4wt%的纤维素溶液;
(3)在步骤(2)的纤维素溶液中加入浓度为10~20wt%的胶态二氧化硅CSPs稀释液,得到混合溶液;此时抗菌剂在混合溶液中的浓度为100~500μg/ml;
(4)混合搅拌,于25~45℃下加热3~10min,经物理交联作用制成抗菌纤维素水凝胶。
本发明中,步骤(3)中的胶态二氧化硅CSPs稀释液的制备方法是:向浓度为50wt%的胶态二氧化硅CSPs(购自sigma公司)中加入蒸馏水,配制成浓度为10~20wt%胶态二氧化硅CSPs稀释液。
本发明的抗菌纤维素水凝胶的制备采取的是物理交联的方法,混合纤维素通过动态和多价的选择性吸附作用连接至二氧化硅纳米颗粒上,用这种方法制备的凝胶材料有很多优点,如不需添加交联剂,因此该水凝胶具有生物相容性更佳,低毒甚至无毒,同时易降解等优点。这类凝胶材料更适合用作与人体有亲密接触的医用材料或食品包装材料。本发明利用上述水凝胶作为载体,加载抗菌剂药物,制得的复合型水凝胶可以用于细菌性感染的局部治疗。
本发明中所述混合纤维素选自羟乙基纤维素HEC和甲基纤维素MC。
所述羟乙基纤维素和甲基纤维素的用量比为3:1~5:1。
所述抗菌剂选自环丙沙星。
本发明的抗菌纤维素水凝胶包含两种廉价而丰富的基本原料,一种是取自木屑、农作物秸秆等天然材料的羟乙基纤维素(HEC)和甲基纤维素(MC),另一种是取自沙子的胶态二氧化硅纳米粒子(CSPs)。这种简单廉价的工艺有望克服目前造价较高的限制,使以工业规模生产水凝胶成为可能。加上伸缩性强、无毒环保等特点,本发明的水凝胶材料有广泛应用前景。
本发明的另一个目的在于提供上述制备方法制备的抗菌纤维素水凝胶的应用,所述抗菌纤维素水凝胶应用于伤口辅料医疗卫生领域。
所述抗菌纤维素水凝胶在制备细菌性感染的局部治疗药物中的应用。
所述抗菌纤维素水凝胶对革兰氏阴性菌大肠杆菌和革兰氏阳性菌枯草芽孢杆菌的抑菌作用。
有益效果:
(1)本发明抗菌纤维素水凝胶的制备工艺简单,该水凝胶的制备采取的是物理交联的方法,纤维素衍生物通过动态和多价的选择性吸附作用连接至二氧化硅纳米颗粒上,同时加载抗菌剂药物,经物理交联作用即可成胶,适合于大规模工业生产。本发明将抗菌剂非共价地负载到凝胶网络中,用于其局部控制释放到组织,它们一旦被水凝胶负载,可以在伤口附近局部使用,是减少用药剂量和抵抗耐药性的好方法。
(2)本发明制备的水凝胶包含两种基本原料,一种是取自木屑、农作物秸秆等天然材料的羟乙基纤维素(HEC)和甲基纤维素(MC),另一种是取自沙子的胶态二氧化硅纳米粒子(CSPs)。这两种原料廉价而丰富,有望克服目前造价较高的限制,使以工业规模生产水凝胶成为可能。
(3)本发明中选取的原料对人体无毒副作用,适合用作与人体有亲密接触的医用材料,其为抗菌材料的开发提供了一个新的有用的起点。
(4)本发明中选取的抗菌剂环丙沙星作为一种新型广谱抗菌药,优点是毒性低、抗菌谱广,抑菌能力强而迅速。将环丙沙星负载在纤维素水凝胶中,能够针对性地应用于局部感染部位,有助于减少用药剂量,并延缓耐药性的出现,因此负载环丙沙星的纤维素水凝胶具有很大的临床应用潜力。
(5)本发明中将环丙沙星负载到纤维素水凝胶后,环丙沙星得到了缓释,从而延长了药物作用时间,也可以减少这类抗生素的使用。
(6)本发明中的水凝胶对革兰氏阴性菌大肠杆菌和革兰氏阳性菌枯草芽孢杆菌都具有良好的抑菌效果。从实际应用角度出发,该水凝胶可以应用于伤口辅料等医疗卫生领域。
附图说明
图1为抗菌纤维素水凝胶对大肠杆菌的抑制生长曲线;
图2为抗菌纤维素水凝胶对枯草芽孢杆菌的抑制生长曲线。
具体实施方式
下面通过具体实施例和附图对本发明技术方案进行详细说明,但是本发明的保护范围不局限于所述实施例。下列实施例中未注明具体条件的实验方法,通常按照常规条件,或按照制造厂商所建议的条件。所用原料及试剂均为市售。
实施例1:
(1)称取0.2g环丙沙星,加蒸馏水至10ml,得到浓度为0.02g/ml的环丙沙星溶液,将其稀释100倍,最终得到浓度为200μg/ml的环丙沙星溶液。环丙沙星浓度为200μg/ml,浓度极低,接近水的密度,可以看做1ml环丙沙星溶液质量为1g。
(2)分别称取0.025g羟乙基纤维素HEC和0.005g甲基纤维素MC,羟乙基纤维素和甲基纤维素的用量比5:1。室温在EP管中充分混合均匀后,将其溶解于1ml浓度为200μg/ml的环丙沙星溶液中,制成浓度3wt%的纤维素溶液;
(3)取400mg浓度为50wt%的胶态二氧化硅CSPs(密度1.4g/ml,sigma公司),滴加蒸馏水至溶液总质量为2g,得到浓度为10wt%胶态二氧化硅CSPs稀释液;
(4)在步骤(2)的纤维素溶液中,滴加步骤(3)的胶态二氧化硅CSPs稀释液,得到混合溶液2ml;此时抗菌剂在混合溶液中的浓度为100μg/ml;
(5)混合搅拌,于37℃下加热8min,经物理交联作用制成抗菌纤维素水凝胶。
本实施例中,环丙沙星在抗菌纤维素水凝胶中的浓度为100μg/ml。
本发明经过上述制备过程形成抗菌纤维素水凝胶后,取样进行抗菌效果测试对比:
A对大肠杆菌的抗菌效果测试
将2%的大肠杆菌接种到装有40mlLB液体培养基的锥形瓶中。将0.1g抗菌纤维素水凝胶加入接种后的LB培养基中,对照例为自然状态下生长的大肠杆菌。每个锥形瓶中取1ml菌液,用分光光度计测菌液的初始OD600。将培养基置于摇床中37℃、160rpm培养8h。每隔1小时取1ml菌液,测定菌液的OD600,并绘制生长抑制曲线,如图1所示。对照例中大肠杆菌处于自然状态,其逐渐生长,OD600值随着时间增加而升高。本发明实施例1的纤维素水凝胶在4h内OD600值逐渐增加,表明大肠杆菌处于增殖状态,但生长速率比对照例缓慢;4h后OD600值保持稳定,表明大肠杆菌停止生长,本发明纤维素水凝胶实施例抗菌效果实现。
B对枯草芽孢杆菌的抗菌效果测试
将2%的枯草芽孢杆菌接种到装有40mlLB液体培养基的锥形瓶中。将0.1g抗菌纤维素水凝胶加入接种后的LB培养基中,对照例为自然状态下生长的枯草芽孢杆菌。每个锥形瓶中取1ml菌液,用分光光度计测菌液的初始OD600。将对照例和不同处理组的培养基置于摇床中37℃、160rpm培养8h。每隔1小时取1ml菌液,测定菌液的OD600,并绘制生长抑制曲线,如图2所示。对照例中枯草芽孢杆菌处于自然状态,其逐渐生长,OD600值随着时间增加而升高。本发明实施例1中纤维素水凝胶5h内OD600值逐渐增加,表明枯草芽孢杆菌处于增殖状态,但生长速率比对照例缓慢;5h后OD600值保持稳定,表明枯草芽孢杆菌停止生长,本发明纤维素水凝胶实施例抗菌效果实现。
实施例2:
(1)称取0.4g环丙沙星,加蒸馏水至10ml,得到浓度为0.04g/ml的环丙沙星溶液,将其稀释100倍,最终得到浓度为400μg/ml的环丙沙星溶液。环丙沙星浓度为400μg/ml,浓度极低,接近水的密度,可以看做1ml环丙沙星溶液质量为1g。
(2)分别称取0.015g羟乙基纤维素HEC和0.005g甲基纤维素MC,羟乙基纤维素和甲基纤维素的用量比3:1。室温在EP管中充分混合均匀后,将其溶解于1ml浓度为400μg/ml的环丙沙星溶液中,制成浓度2wt%的纤维素溶液;
(3)取480mg浓度为50wt%的胶态二氧化硅CSPs(密度1.4g/ml,sigma公司),滴加蒸馏水至溶液总质量为2g,得到浓度为12wt%胶态二氧化硅CSPs稀释液;
(4)在步骤(2)的纤维素溶液中,滴加步骤(3)的胶态二氧化硅稀释液,得到混合溶液2ml;此时抗菌剂在混合溶液中的浓度为200μg/ml;
(5)混合搅拌,于25℃下加热10min,经物理交联作用制成抗菌纤维素水凝胶。
本实施例中环丙沙星在抗菌纤维素水凝胶中的浓度为200μg/ml。
本发明经过上述制备过程形成抗菌纤维素水凝胶后,取样进行抗菌效果测试对比:
A对大肠杆菌的抗菌效果测试
将2%的大肠杆菌接种到装有40mlLB液体培养基的锥形瓶中。将0.1g水凝胶加入接种后的LB培养基中,对照例为自然状态下生长的大肠杆菌。每个锥形瓶中取1ml菌液,用分光光度计测菌液的初始OD600。将对照例和不同处理组的培养基置于摇床中37℃、160rpm培养8h。每隔1小时取1ml菌液,测定菌液的OD600,并绘制生长抑制曲线,如图1所示。对照例中大肠杆菌处于自然状态,其逐渐生长,OD600值随着时间增加而升高。本发明实施例2纤维素水凝胶在4h内OD600值逐渐增加,表明大肠杆菌处于增殖状态,但生长速率比对照例缓慢;4h后OD600值保持稳定,表明大肠杆菌停止生长,本发明纤维素水凝胶实施例抗菌效果实现。
B对枯草芽孢杆菌的抗菌效果测试
将2%的枯草芽孢杆菌接种到装有40mlLB液体培养基的锥形瓶中。将0.1g水凝胶加入接种后的LB培养基中,对照例为自然状态下生长的枯草芽孢杆菌。每个锥形瓶中取1ml菌液,用分光光度计测菌液的初始OD600。将对照例和不同处理组的培养基置于摇床中37℃、160rpm培养8h。每隔1小时取1ml菌液,测定菌液的OD600,并绘制生长抑制曲线,如图2所示。对照例中枯草芽孢杆菌处于自然状态,其逐渐生长,OD600值随着时间增加而升高。本发明实施例2中纤维素水凝胶5h内OD600值逐渐增加,表明枯草芽孢杆菌处于增殖状态,但生长速率比对照例缓慢;5h后OD600值保持稳定,表明枯草芽孢杆菌停止生长,本发明纤维素水凝胶实施例抗菌效果实现。
实施例3:
(1)称取0.5g环丙沙星,加蒸馏水至10ml,得到浓度为0.05g/ml的环丙沙星溶液,将其稀释100倍,最终得到浓度为500μg/ml的环丙沙星溶液。环丙沙星浓度为500μg/ml,浓度极低,接近水的密度,可以看做1ml环丙沙星溶液质量为1g。
(2)分别称取0.02g羟乙基纤维素HEC和0.005g甲基纤维素MC,羟乙基纤维素和甲基纤维素的用量比4:1。室温在EP管中充分混合均匀后,将其溶解于1ml浓度为500μg/ml的环丙沙星溶液中,制成浓度2.5wt%的纤维素溶液;
(3)取800mg浓度为50wt%的胶态二氧化硅CSPs(密度1.4g/ml,sigma公司),滴加蒸馏水至溶液总质量为2g,得到浓度为20wt%胶态二氧化硅CSPs稀释液;
(4)在步骤(2)的纤维素溶液中,滴加步骤(3)的胶态二氧化硅稀释液,得到混合溶液2ml;此时抗菌剂在混合溶液中的浓度为250μg/ml;
(5)混合搅拌,于30℃下加热6min,经物理交联作用制成抗菌纤维素水凝胶。
本实施例中环丙沙星在抗菌纤维素水凝胶中的浓度为250μg/ml;
本发明经过上述制备过程形成抗菌纤维素水凝胶后,取样进行抗菌效果测试对比:
A对大肠杆菌的抗菌效果测试
将2%的大肠杆菌接种到装有40mlLB液体培养基的锥形瓶中。将0.1g水凝胶加入接种后的LB培养基中,对照例为自然状态下生长的大肠杆菌。每个锥形瓶中取1ml菌液,用分光光度计测菌液的初始OD600。将对照例和不同处理组的培养基置于摇床中37℃、160rpm培养8h。每隔1小时取1ml菌液,测定菌液的OD600,并绘制生长抑制曲线,如图1所示。对照例中大肠杆菌处于自然状态,其逐渐生长,OD600值随着时间增加而升高。本发明实施例3纤维素水凝胶在4h内OD600值逐渐增加,表明大肠杆菌处于增殖状态,但生长速率比对照例缓慢;4h后OD600值保持稳定,表明大肠杆菌停止生长,本发明纤维素水凝胶实施例抗菌效果实现。
B对枯草芽孢杆菌的抗菌效果测试
将2%的枯草芽孢杆菌接种到装有40mlLB液体培养基的锥形瓶中。将0.1g水凝胶加入接种后的LB培养基中,对照例为自然状态下生长的枯草芽孢杆菌。每个锥形瓶中取1ml菌液,用分光光度计测菌液的初始OD600。将对照例和不同处理组的培养基置于摇床中37℃、160rpm培养8h。每隔1小时取1ml菌液,测定菌液的OD600,并绘制生长抑制曲线,如图2所示。对照例中枯草芽孢杆菌处于自然状态,其逐渐生长,OD600值随着时间增加而升高。本发明实施例3中纤维素水凝胶5h内OD600值逐渐增加,表明枯草芽孢杆菌处于增殖状态,但生长速率比对照例缓慢;5h后OD600值保持稳定,表明枯草芽孢杆菌停止生长,本发明纤维素水凝胶实施例抗菌效果实现。
实施例4:
(1)称取1g环丙沙星,加蒸馏水至10ml,得到浓度为0.1g/ml的环丙沙星溶液,将其稀释100倍,最终得到浓度为1000μg/ml的环丙沙星溶液。环丙沙星浓度为1000μg/ml,浓度极低,接近水的密度,可以看做1ml环丙沙星溶液质量为1g。
(2)分别称取0.03g羟乙基纤维素HEC和0.01g甲基纤维素MC,羟乙基纤维素和甲基纤维素的用量比3:1。室温在EP管中充分混合均匀后,将其溶解于1ml浓度为1000μg/ml的环丙沙星溶液中,制成浓度4wt%的纤维素溶液;
(3)取600mg浓度为50wt%的胶态二氧化硅CSPs(密度1.4g/ml,sigma公司),滴加蒸馏水至溶液总质量为2g,得到浓度为15wt%胶态二氧化硅CSPs稀释液;
(4)在步骤(2)的纤维素溶液中,滴加步骤(3)的胶态二氧化硅稀释液,得到混合溶液2ml;此时抗菌剂在混合溶液中的浓度为500μg/ml;
(5)混合搅拌,于42℃下加热4min,经物理交联作用制成抗菌纤维素水凝胶。
本实施例中环丙沙星在抗菌纤维素水凝胶中的浓度为500μg/ml。
本发明经过上述制备过程形成抗菌纤维素水凝胶后,取样进行抗菌效果测试对比:
A对大肠杆菌的抗菌效果测试
将2%的大肠杆菌接种到装有40mlLB液体培养基的锥形瓶中。将0.1g水凝胶加入接种后的LB培养基中,对照例为自然状态下生长的大肠杆菌。每个锥形瓶中取1ml菌液,用分光光度计测菌液的初始OD600。将对照例和不同处理组的培养基置于摇床中37℃、160rpm培养8h。每隔1小时取1ml菌液,测定菌液的OD600,并绘制生长抑制曲线,如图1所示。对照例中大肠杆菌处于自然状态,其逐渐生长,OD600值随着时间增加而升高。本发明实施例4纤维素水凝胶在4h内OD600值逐渐增加,表明大肠杆菌处于增殖状态,但生长速率比对照例缓慢;4h后OD600值保持稳定,表明大肠杆菌停止生长,本发明纤维素水凝胶实施例抗菌效果实现。
B对枯草芽孢杆菌的抗菌效果测试
将2%的枯草芽孢杆菌接种到装有40mlLB液体培养基的锥形瓶中。将0.1g水凝胶加入接种后的LB培养基中,对照例为自然状态下生长的枯草芽孢杆菌。每个锥形瓶中取1ml菌液,用分光光度计测菌液的初始OD600。将对照例和不同处理组的培养基置于摇床中37℃、160rpm培养8h。每隔1小时取1ml菌液,测定菌液的OD600,并绘制生长抑制曲线,如图2所示。对照例中枯草芽孢杆菌处于自然状态,其逐渐生长,OD600值随着时间增加而升高。本发明实施例4中纤维素水凝胶5h内OD600值逐渐增加,表明枯草芽孢杆菌处于增殖状态,但生长速率比对照例缓慢;5h后OD600值保持稳定,表明枯草芽孢杆菌停止生长,本发明纤维素水凝胶实施例抗菌效果实现。
实施例5:
(1)称取0.8g环丙沙星,加蒸馏水至10ml,得到浓度为0.08g/ml的环丙沙星溶液,将其稀释100倍,最终得到浓度为800μg/ml的环丙沙星溶液。环丙沙星浓度为800μg/ml,浓度极低,接近水的密度,可以看做1ml环丙沙星溶液质量为1g。
(2)分别称取0.032g羟乙基纤维素HEC和0.008g甲基纤维素MC,羟乙基纤维素和甲基纤维素的用量比4:1。室温在EP管中充分混合均匀后,将其溶解于1ml浓度为800μg/ml的环丙沙星溶液中,制成浓度4wt%的纤维素溶液;
(3)取680mg浓度为50wt%的胶态二氧化硅CSPs(密度1.4g/ml,sigma公司),滴加蒸馏水至溶液总质量为2g,得到浓度为17wt%胶态二氧化硅CSPs稀释液;
(4)在步骤(2)的纤维素溶液中,滴加步骤(3)的胶态二氧化硅稀释液,得到混合溶液2ml;此时抗菌剂在混合溶液中的浓度为400μg/ml;
(5)混合搅拌,于45℃下加热3min,经物理交联作用制成抗菌纤维素水凝胶。
本实施例中环丙沙星在抗菌纤维素水凝胶中的浓度为400μg/ml。
本发明经过上述制备过程形成抗菌纤维素水凝胶后,取样进行抗菌效果测试对比:
A对大肠杆菌的抗菌效果测试
将2%的大肠杆菌接种到装有40mlLB液体培养基的锥形瓶中。将0.1g水凝胶加入接种后的LB培养基中,对照例为自然状态下生长的大肠杆菌。每个锥形瓶中取1ml菌液,用分光光度计测菌液的初始OD600。将对照例和不同处理组的培养基置于摇床中37℃、160rpm培养8h。每隔1小时取1ml菌液,测定菌液的OD600,并绘制生长抑制曲线,如图1所示。对照例中大肠杆菌处于自然状态,其逐渐生长,OD600值随着时间增加而升高。本发明实施例5纤维素水凝胶在3h内OD600值逐渐增加,表明大肠杆菌处于增殖状态,但生长速率比对照例缓慢;3h后OD600值保持稳定,表明大肠杆菌停止生长,本发明纤维素水凝胶实施例抗菌效果实现。
B对枯草芽孢杆菌的抗菌效果测试
将2%的枯草芽孢杆菌接种到装有40mlLB液体培养基的锥形瓶中。将0.1g水凝胶加入接种后的LB培养基中,对照例为自然状态下生长的枯草芽孢杆菌。每个锥形瓶中取1ml菌液,用分光光度计测菌液的初始OD600。将对照例和不同处理组的培养基置于摇床中37℃、160rpm培养8h。每隔1小时取1ml菌液,测定菌液的OD600,并绘制生长抑制曲线,如图2所示。对照例中枯草芽孢杆菌处于自然状态,其逐渐生长,OD600值随着时间增加而升高。本发明实施例5中纤维素水凝胶4h内OD600值逐渐增加,表明枯草芽孢杆菌处于增殖状态,但生长速率比对照例缓慢;4h后OD600值保持稳定,表明枯草芽孢杆菌停止生长,本发明纤维素水凝胶实施例抗菌效果实现。
由此可见,本发明的抗菌纤维素水凝胶对革兰氏阴性菌大肠杆菌和革兰氏阳性菌枯草芽孢杆菌有良好的抑菌效果,所述抗菌纤维素水凝胶能够应用在制备细菌性感染的局部治疗药物方面,可以应用于伤口辅料等医疗卫生领域。
如上所述,尽管参照特定的优选实施例已经表示和表述了本发明,但其不得解释为对本发明自身的限制。在不脱离所附权利要求定义的本发明的精神和范围前提下,可对其在形式上和细节上作出各种变化。
Claims (7)
1.一种抗菌纤维素水凝胶的制备方法,其特征在于,包括以下步骤:
(1)配制浓度为200~1000μg/ml的抗菌剂溶液;
(2)将混合纤维素原料室温充分混合均匀后,溶解于所述抗菌剂溶液中,制成浓度2~4wt%的纤维素溶液;
(3)在步骤(2)的纤维素溶液中加入浓度为10~20wt%的胶态二氧化硅CSPs稀释液,得到混合溶液;此时抗菌剂在混合溶液中的浓度为100~500μg/ml;
(4)混合搅拌,于25~45℃下加热3~10min,经物理交联作用制成抗菌纤维素水凝胶。
2.根据权利要求1所述的制备方法,其特征在于,所述混合纤维素选自羟乙基纤维素HEC和甲基纤维素MC。
3.根据权利要求2所述的制备方法,其特征在于,所述羟乙基纤维素和甲基纤维素的用量比为3:1~5:1。
4.根据权利要求1所述的制备方法,其特征在于,所述抗菌剂选自环丙沙星。
5.权利要求1~4任一项所述的制备方法制备的抗菌纤维素水凝胶的应用,其特征在于,所述抗菌纤维素水凝胶应用于伤口辅料医疗卫生领域。
6.根据权利要求5所述的应用,其特征在于,所述抗菌纤维素水凝胶在制备细菌性感染的局部治疗药物中的应用。
7.根据权利要求6所述的应用,其特征在于,所述抗菌纤维素水凝胶对革兰氏阴性菌大肠杆菌和革兰氏阳性菌枯草芽孢杆菌的抑菌作用。
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