CN110199805A - A kind of rice paddy seed pure keeping propagation method - Google Patents
A kind of rice paddy seed pure keeping propagation method Download PDFInfo
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- CN110199805A CN110199805A CN201910626927.9A CN201910626927A CN110199805A CN 110199805 A CN110199805 A CN 110199805A CN 201910626927 A CN201910626927 A CN 201910626927A CN 110199805 A CN110199805 A CN 110199805A
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- Prior art keywords
- seed
- rice
- stub
- seedling
- rice stub
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/20—Cereals
- A01G22/22—Rice
Abstract
The invention discloses a kind of rice paddy seed pure keeping propagation methods, and the seed on target rice stub is first harvested to while being retained its correspondence rice stub of cultivation, and the characteristic of the seed sowing observation first generation seedling of harvest selects the standard compliant first generation with this；Harvest first generation seedling seed is sowed again, is observed the characteristic of second generation seedling with this final election and is gone out standard compliant first generation seed, and then goes out it with this final election and correspond to rice stub.Rice stub cultivation is corresponded to again, carry out regenerating tiller and divides the root and stem of certain plants, acquires sufficient amount of regrowth, and the seed harvested on regrowth repeatedly plants a generation in this, as original seed, and by original seed again, harvests its seed and is used as in production with kind.This method becomes the purification repeatedly of original seed to obtain the consistent original seed regrowth of genotype repeatedly using the infinite regeneration of rice, simplifying seed production with this.
Description
Technical field
The invention belongs to rice paddy seed purifications, reproduction technique field, and in particular to a kind of rice paddy seed pure keeping propagation method.
Background technique
The generic way of rice paddy seed breeding is next-generation with seed sowing plantation and harvest is new seed.It is used in production
Seed be all to guarantee seed amount by repeatedly breeding, due to during seminal propagation several factors can generate it is miscellaneous
Strain, the source for breeding hybrid strain in work is very extensive, and the pure keeping technical difficulty of seminal propagation is very high.Especially biology mixes and loses
Passing the hybrid strain that drift generates is more difficult to remove, as the fertility origin temp of two-line sterile line can become with the increase of breeding generation
Height, risk when causing two to be the production of hybrid seeds.Pure keeping technology in seed production is particularly significant.
It is seven garden methods that conventional pure keeping technology, which has three, and hereditary method of purification etc., basic principle is to generate in reproductive process
Variant carry out test repeatedly and verify and obtain after removing original seed, then expanding propagation into production kind.Due to the output of original seed
It is limited, it is necessary to be that this Purification is repeated just to can guarantee original seed quantity, Purification is very many and diverse, and technical requirements
It is high.
The present invention utilizes the unlimited shooting property of rice, obtains regrowth largely repeatedly to guarantee original seed quantity, harvest is again
The seed of raw seedling expands numerous primary acquisition production kind as original seed.The long-term seed for retaining regrowth and harvesting regrowth, can
Guarantee that the genotype of original seed is consistent, original seed, which only expands a numerous generation, reduces seminal propagation generation, reduces biology and mixes and lose
Pass the risk of drift.It is a kind of ideal seed pure keeping method.
Summary of the invention
The technical scheme adopted by the invention is that: a kind of rice paddy seed pure keeping propagation method is provided, which is characterized in that including
Following steps:
(1) rice stub of target variety is obtained and is cultivated: being selected elite seedling sowing when target variety maturation, is prescinded liftoff 5-
10 centimetres or more of stalk, rice stub is transplanted in the rice seedling bed for facilitating management, spreads fertilizer over the fields nitrogenous fertilizer by 10~15kg/ mus of dosage, together
When spray insecticide；In rice stub cultivating process, keep field wet；
(2) primary election of original seed rice stub: the seed harvested in rice strain is sowed, the characteristic of first generation seedling is observed, selects
Meet the rice stub of breed standard.And harvest the seed of first generation seedling；
(3) production kind rice stub final election: the seed harvested on the first generation seedling of primary election qualification is continued to sow, observation second
For the characteristic of seedling, final election goes out to meet the second generation seedling of breed standard, chases after comprehensive corresponding original seed rice stub.
(4) original seed produces: using the rice stub gone out by the above three-step process final election as original seed provenance, repeatedly by rice stub
Ratooning rice cultivation is carried out, harvests the solid seed of its regrowth institute as kind original seed；And rice stub is retained and cultivated for a long time
Regrowth obtains continual original seed；
(5) production kind seed production: original seed is sowed, and is carried out cultivation management by kind Cultivation measures for high yield, is received
Seed is as production kind.
Further, rice stub root system surface is coated with the old soil with a thickness of 3~5cm when rice stub is transplanted in step (1).The present invention
In rice stub in transplanting, rice stub root system is coated by old soil, containing growing the substances such as beneficial microorganism, rice to rice stub in old soil
Stake is transplanted together with old soil, can promote down the survival rate of stake.
Further, in step (1) insecticide used be 10% Buprofezin missible oil 1000 times of liquid.
Further, the seed manure used in step (1) is nitrogenous fertilizer, is urea or compound fertilizer.
Further, rice stub cultivation work simply continues to carry out qualified seedling at the end of implementation steps (2) in step (1)
The cultivation of corresponding rice stub.Remaining rice stub does not continue to cultivate.
Further, rice stub cultivates work in step (1), at the end of implementation steps (3), simply continues to carry out qualified kind again
The cultivation of the correspondence rice stub of seedling.Remaining rice stub does not continue to cultivate.
Further, the seedling characteristic observation method in step (3) and step (2) are as follows: every part of strain at least plants 1000 plants
Above and according to breed standard controlled observation one by one, with completely up to standard for final election foundation.
Further, the long-term reservation method of regrowth is the ground grown in Hainan or other suitable rice anniversaries in step (4)
Area or facility.
The beneficial effects of the present invention are:
1. the purification of original seed is the same with conventional original seed purification in the present invention, but after the corresponding rice stub of acquisition original seed just no longer
Continue original seed purification repeatedly, but rice stub is utilized to cultivate regrowth, and regrowth is cultivated repeatedly and obtains a large amount of genotypes
Consistent regrowth, because its solid seed genotype it is consistent, avoid biology and mix generation with genetic drift, from basic
On solve the purity of original seed, be a kind of ideal seed pure keeping method.
2. the present invention using the seed of regrowth as original seed, original seed is only expanded it is numerous once, can be effectively reduced more
Secondary breeding necessarily leads to biology and mixes and genetic drift, and can largely obtain production and plant and reduce cost.It can guarantee that production is used
The large supply of kind.
3. the present invention is being produced with kind for having been completed that breediness is observed before producing, the characteristic symbol of seed can be ensured
Close breed standard.Ensure the safety of seed production.
4. in mutation mostly generation, by the consistent rice stub conservation of previous generation's heredity, can make to contain from numerous using the method in the present invention
There is the part kind of the not pure and mild gene of minor effect to be able to apply in production.The fertility of especially controllable two-line sterile line plays point drift
Become, keep the work of two-line sterile line pure keeping more reliable and becomes easy.
Specific embodiment
Below with reference to embodiment, specific embodiments of the present invention will be described in detail.
Embodiment 1
A kind of rice paddy seed pure keeping propagation method, comprising the following steps:
(1) rice stub of target variety is obtained and is cultivated: being selected elite seedling sowing when target variety maturation, is prescinded liftoff 5-
10 centimetres or more of stalk, rice stub is transplanted in the rice seedling bed for facilitating management, spreads fertilizer over the fields nitrogenous fertilizer by 10~15kg/ mus of dosage, together
When spray insecticide；In rice stub cultivating process, keep field wet；
(2) primary election of original seed rice stub: the seed plant division reference numeral that will be harvested on rice strain and rice stub, in kind Production Zones Suitable
Suitable Sowing Period sowing, every part of seed plants 1000 plants by variety culture technical specification, and the timesharing phase observes first generation seedling
Characteristic, and comparing with breed standard, selects the number for meeting the first generation seedling of breed standard, find out corresponding rice stub after
Continuous to cultivate regrowth, remaining rice stub is eliminated.The seed of first generation seedling is harvested with spare；
(3) production kind rice stub final election: the seed harvested on the first generation seedling of primary election qualification is continued to sow, observation second
For the characteristic of seedling, final election goes out to meet the second generation seedling of breed standard.Sowing, observation, final election method are same as above (2).Chase after it is comprehensive go out
Corresponding original seed rice stub simultaneously continues to cultivate regrowth, remaining rice stub is eliminated.
(4) original seed produces: using the rice stub gone out by the above three-step process final election as original seed provenance, repeatedly by rice stub
Ratooning rice cultivation is carried out, harvests the solid seed of its regrowth institute as kind original seed；And by rice stub on Sanya, Hainan and other places
The head of district retains and cultivates regrowth the phase, obtains continual original seed；
(5) production kind seed production: original seed is sowed in kind Production Zones Suitable, is carried out by kind Cultivation measures for high yield
Cultivation management, institute's sowing is as production kind.
Embodiment 2
A kind of rice paddy seed pure keeping propagation method, comprising the following steps:
(1) rice stub of target variety is obtained and is cultivated: being selected elite seedling sowing when target variety maturation, is prescinded liftoff 5-
10 centimetres or more of stalk, rice stub is transplanted in the rice seedling bed for facilitating management, spreads fertilizer over the fields nitrogenous fertilizer by 10~15kg/ mus of dosage, together
When spray insecticide；In rice stub cultivating process, keep field wet；
(2) primary election of original seed rice stub: the seed plant division reference numeral that will be harvested on rice stub and rice strain, in kind Production Zones Suitable
Suitable Sowing Period sowing, every part of seed plants 1000 plants by variety culture technical specification, and the timesharing phase observes first generation seedling
Characteristic, and comparing with breed standard, selects the number for meeting the first generation seedling of breed standard, find out corresponding rice stub after
Continuous to cultivate regrowth, remaining rice stub is eliminated.The seed of first generation seedling is harvested with spare；
(3) production kind rice stub final election: the spare seed harvested on the first generation seedling of primary election qualification is continued to sow, and is observed
The characteristic of second generation seedling, final election go out to meet the second generation seedling of breed standard, and sowing, observation, final election method are same as above (2).It chases after
Comprehensive corresponding original seed rice stub out simultaneously continues to cultivate regrowth, remaining rice stub is eliminated.
(4) original seed produces: using the rice stub gone out by the above three-step process final election as original seed provenance, repeatedly by rice stub
Ratooning rice cultivation is carried out, harvests the solid seed of its regrowth institute as kind original seed；And it is rice stub is long in artificial greenhouse
Phase retains and cultivates regrowth in general big Tanaka, obtains continual original seed；
(5) production kind seed production: original seed is sowed in general big Tanaka, is planted by kind Cultivation measures for high yield
Training management, institute's sowing is as production kind.
Claims (8)
1. a kind of rice paddy seed pure keeping propagation method, which comprises the following steps:
(1) rice stub of target variety is obtained and is cultivated: being selected elite seedling sowing when target variety maturation, is prescinded liftoff 5-10 lis
Meter or more stalk, rice stub is transplanted in the rice seedling bed for facilitating management, seed manure is spread fertilizer over the fields by 10~15kg/ mus of dosage, sprays simultaneously
Apply insecticide；In rice stub cultivating process, keep field wet；
(2) primary election of original seed rice stub: the seed harvested in rice strain is sowed, the characteristic of first generation seedling is observed, selects and meet
The rice stub of breed standard；And harvest the seed of first generation seedling；
(3) production kind rice stub final election: the seed harvested on the first generation seedling of primary election qualification is continued to sow, and observes second generation kind
The characteristic of seedling, final election go out to meet the second generation seedling of breed standard, chase after comprehensive corresponding original seed rice stub；
(4) original seed produces: using the rice stub gone out by the above three-step process final election as original seed provenance, repeatedly carrying out rice stub
Regrowth is cultivated, and harvests the solid seed of its regrowth institute as kind original seed；And rice stub is retained for a long time and cultivates regeneration
Seedling obtains continual original seed；
(5) production kind seed production: original seed is sowed, and carries out cultivation management, institute's sowing by kind Cultivation measures for high yield
As production kind.
2. a kind of rice paddy seed pure keeping propagation method according to claim 1, it is characterised in that: rice stub moves in step (1)
The old soil that rice stub root system surface is coated with a thickness of 3~5cm when cultivation.
3. a kind of rice paddy seed pure keeping propagation method according to claim 1, it is characterised in that: used in step (1) to kill
Worm agent is 1000 times of liquid of 10% Buprofezin missible oil.
4. a kind of rice paddy seed pure keeping propagation method according to claim 1, it is characterised in that: used in step (1)
Seed manure is nitrogenous fertilizer, is urea or compound fertilizer.
5. a kind of rice paddy seed pure keeping propagation method according to claim 1, it is characterised in that: rice stub is trained in step (1)
Work is educated, at the end of implementation steps (2), simply continues to the cultivation for the correspondence rice stub for carrying out qualified seedling, remaining rice stub is no longer
Continue to cultivate.
6. a kind of rice paddy seed pure keeping propagation method according to claim 1, it is characterised in that: rice stub is trained in step (1)
Work is educated, at the end of implementation steps (3), simply continues to carry out the cultivation of the correspondence rice stub of qualified seedling again, remaining rice stub
It does not continue to cultivate.
7. a kind of rice paddy seed pure keeping propagation method according to claim 1, it is characterised in that: step (3) and step (2)
In seedling characteristic observation method are as follows: every part of strain at least plants 1000 plants or more and according to breed standard controlled observation one by one,
With completely up to standard for final election foundation.
8. a kind of rice paddy seed pure keeping propagation method according to claim 1, it is characterised in that: regrowth in step (4)
Long-term reservation method is in the area or facility of Hainan or other suitable rice anniversary growths.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910626927.9A CN110199805A (en) | 2019-07-12 | 2019-07-12 | A kind of rice paddy seed pure keeping propagation method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910626927.9A CN110199805A (en) | 2019-07-12 | 2019-07-12 | A kind of rice paddy seed pure keeping propagation method |
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| CN110199805A true CN110199805A (en) | 2019-09-06 |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1365607A (en) * | 2001-12-05 | 2002-08-28 | 广东省农业科学院水稻研究所 | Method for breeding super hybridized rice |
| CN1565154A (en) * | 2003-06-24 | 2005-01-19 | 华中农业大学 | Conservation and breeding method for rice photosensitive and temperature-sensitive sterile line |
| CN102119640A (en) * | 2010-11-19 | 2011-07-13 | 湖南农业大学 | Method for producing rice dual-purpose nucleic sterile line original seed |
| CN102498998A (en) * | 2011-11-24 | 2012-06-20 | 湖南杂交水稻研究中心 | Method for purification, retainment and propagation of seeds of rice photo-thermo-sensitive male sterile line |
| CN109588239A (en) * | 2018-12-29 | 2019-04-09 | 雅安雨禾农业科技有限责任公司 | A kind of multiple rice regeneration rice breeding method |
| CN109618920A (en) * | 2019-02-23 | 2019-04-16 | 重庆市农业科学院 | A kind of hybrid paddy rice dual purpose genic male sterile line clonal regeneration pure keeping method |
-
2019
- 2019-07-12 CN CN201910626927.9A patent/CN110199805A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1365607A (en) * | 2001-12-05 | 2002-08-28 | 广东省农业科学院水稻研究所 | Method for breeding super hybridized rice |
| CN1565154A (en) * | 2003-06-24 | 2005-01-19 | 华中农业大学 | Conservation and breeding method for rice photosensitive and temperature-sensitive sterile line |
| CN102119640A (en) * | 2010-11-19 | 2011-07-13 | 湖南农业大学 | Method for producing rice dual-purpose nucleic sterile line original seed |
| CN102498998A (en) * | 2011-11-24 | 2012-06-20 | 湖南杂交水稻研究中心 | Method for purification, retainment and propagation of seeds of rice photo-thermo-sensitive male sterile line |
| CN109588239A (en) * | 2018-12-29 | 2019-04-09 | 雅安雨禾农业科技有限责任公司 | A kind of multiple rice regeneration rice breeding method |
| CN109618920A (en) * | 2019-02-23 | 2019-04-16 | 重庆市农业科学院 | A kind of hybrid paddy rice dual purpose genic male sterile line clonal regeneration pure keeping method |
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Application publication date: 20190906 |
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