CN1365607A - Method for breeding super hybridized rice - Google Patents
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Abstract
A method for breeding seed of super hybrid rice "Yueza 122" features that the high-quality photosensitive and thermosensitive nuclear sterile line long-grained nonglutinous rice GD-1S is chosen as female parent and the high-quality disease-resistant recovery line "Guanghui 122" as male parent. The resultant "Yueza 122" features short growth period (112-125 days), high disease-resistance, and relatively good transparency of rice grain.
Description
One. affiliated technical field
The present invention relates to the breeding method of a kind of rice breeding method, particularly a kind of super hybridized rice.Belong to the rice heterosis utilization field.
Two. background technology
Since the eighties, become the domestic and international research focus based on super high-yielding or super hybridization rice (super hybridized rice) breeding that improves output.The Japanese had formulated " contrary 7,5,3 " super high-yielding rice breeding plan in 1981, the super high-yielding kind than original kind volume increase 50% was bred in requirement in 15 years, promptly to nineteen ninety-five, brown rice output is brought up to 7.50-9.75 ton/hectare (amounting to paddy 9.38-12.19 ton/hectare) by original 5.00-6.50 ton/hectare.On technology path, main attack " big fringe is spent more " and " super large grain " both direction.Selected famous kinds such as bright star in northern land 129 (Xiang), northern land 130 (energetically) and plumage difficult to understand 326 by 1989 by indica-japonica hybrid.Land 129, north (Xiang) is big fringe kind, 140.6 of every fringes; 130 in land, north is big grain type kind, and thousand kernel weight is up to 48.1 grams.Although but these kinds reach the volume increase target substantially on small size, all because of fringe lacks greatly, adaptability is undesirable, and large tracts of land fails to realize increasing production 50% target.In addition, also, finally there is not large scale application because rice matter does not meet Japanese national conditions.
International Rice Research Institute (IRRI) proposition in 1989 renames as " novel strain type breeding " plan again after cultivating " super hybridization rice ", and to design the few tiller of big fringe be the novel strain type of main feature, and its specific targets are: tillering ability weak (tillering for 3-4), do not have that thick straight, the well developed root system of ineffective tillering, big fringe (200-250 grain/fringe), plant height 90-100 centimetre, stiff in straw, leaf dark green, anti-multiple damage by disease and insect, the 110-130 days time of infertility, harvest index reach 0.6, yield potentiality is 13-15 ton/hectare.The novel strain type product that IRRI seed selection in 1994 is come out tie up to the torrid zone dry season only cell production reach 12.5 tons/hectare.So far, IRRI selects the novel strain type strain of coming because ripening rate is lower, rice matter resistance is undesirable, and the stable and consistent of colony is poor, fails large-area applications on producing, and just does further improvement at the problems referred to above at present.
China scientist has also successively proposed super high-yielding rice breeding imagination, and has carried out multipath exploration.Yang Shouren etc. have proposed " ideotype " pattern to plant type of rice comparatively on the basis of system research in modern age, and to have put into practice with the big fringe of short bar be the super high-yielding breeding of principal character, have successively bred round-grained rice type short-stalked varietys such as Shen Nong 265 and Shen Nong 366.Huang Yaoxiang has proposed the super high-yielding breeding conception of " short living clump is long " and " it is fast long to grow thickly " according to the ecological condition of South China.And to have bred the higher kind of yield potentiality special blue or green and win etc. excellent No. 2.Nineteen ninety-five Zhou Kaida has proposed the conception of super high-yielding hybrid rice kind according to the breeding practice of ternary hybrid rice High yield combination II excellent 6078.Think and want seed selection than the hybrid rice of Shanyou 63 volume increase more than 15%, technically should be based on seed selection " heavy fringe type " inter-subspecies hybrid rice.
Yuan Longping academician in 1997 has in time proposed the breeding objective and the task of super hybridized rice according to the latest developments that China's two line method inter-subspecific heterosis utilization research obtains.Think that super high-yielding rice yield index is along with era development, ecological area is not different and different with plantation season.Because breeding time, length and unit are absolute yield height was closely related, thought comparatively reasonable to have a daily output of measurement index.Therefore, the yield index of the super hybridized rice breeding of 100 kilograms of the per hectare dailys output has been proposed, and the well-designed plant type pattern of super hybridized rice, emphasize effectively to increase " source ", improving photosynthetic efficiency is the key that realizes super high-yielding.
The Ministry of Agriculture held expert discussion in Shenyang in 1996, the formal startup of decision " Chinese super hybridization rice plan ", and the breeding objective of wishing to realize volume increase 15% in 2000 and realizing volume increase 30% by 2005:
Table 1 the Ministry of Agriculture super hybridization rice yield index (ton/hectare)
| Type | Conventional rice | Hybrid rice | Amount of increase in production | |||||
| Xian early | Morning, noon and afternoon dual-purpose Xian | South single cropping round-grained rice | North round-grained rice | Xian early | Single cropping Xian, round-grained rice | Late Xian | ||
| Existing high yield level | ???6.75 | ????7.50 | ???7.50 | ????8.25 | ??7.50 | ??8.25 | ???7.50 | ??0 |
| ??1996-2000 | ???9.00 | ????9.75 | ???9.75 | ???10.50 | ??9.75 | ??10.50 | ???9.75 | ??15％ |
| ??2000-2005 | ???10.50 | ????11.25 | ???11.25 | ???12.00 | ??11.25 | ??12.00 | ???11.25 | ??30％ |
National high-tech research development plan in 1998 (" 863 " plan) has been organized charrette in Zhaoqing, Guangdong Province, also formally starts " super hybridized rice research " project.Spanning plan in 1999 and premier's fund are all classified the primary study project to " super hybridized rice breeding " as.And obtained certain progress, having brought out some ripe two is the combination of the super hybridized rice symptom of a trend late, as the two line system (training short 64S/9311) of academy of agricultural sciences, Jiangsu with train training two excellent 1826 (the training short 64S/1826) etc. in short 64S/E32, Hubei.It is long that these combinations belong to slow ripe type combination, breeding time, not only not too be fit to the south China double rice cropping system early, the plantation of late two seasons uses, and yet do not reach 100 kilograms/hectare super hybridized rice index by the daily output, have in addition inferior quality and resistance bad.In addition, these two-line hybrid rices all are to utilize the short 64S of training to be the combination that female parent made, and all have the not high shakiness of hybrid seed yield, " 920 " usage amount is big and the breeding cost height, meet unfavorable factors such as the harm of adverse weather smut is comparatively serious.
As the south China double rice cropping system of one of China paddy rice main producing region, because its unique light temperature ecological condition and cropping system, with other rice district, the district differs greatly as Yangtze river basin rice.Therefore, cultivate the super hybridized rice combination that is fit to the south China rice workspace and have crucial meaning.
Three. technology contents
The problem that the present invention need solve, it is purpose of the present invention, just provide a kind of breeding method of super hybridized rice, with seed selection high-quality, disease-resistant, the hybrid rice breeding new material that is fit to South China's light temperature ecological condition, has novel strain type characteristic and super high-yielding potentiality, be fit to super hybridized rice combination that south China Dao Qu uses for producing upward large-area applications to cultivate.
In order to reach this present invention's purpose, can be by taking following measure: a kind of breeding method of super hybridized rice be characterized in: the seed selection plant type is good, coordinate force is strong, origin temp low (≤24 ℃), the stable sterile phase is long, outcrossing seed-setting rate is high, the quality indica photo-thermo-sensitive genic male sterile line that is fit to South China's light temperature ecological condition is female parent; The quality indica superior restorer line is a male parent, is mixed with the super hybridized rice combination, is specifically realized by following steps:
1) the photosensitive-sensitive sterile line W7415S that does to be bred with academy of agricultural sciences, Hubei grain is the sterile gene donor,, high yield good, the special blue or green hybridization of high light efficiency conventional rice kind with the Guangdong plant type, by the shuttle method of breeding of Guangzhou and strange land, Hainan, cultivate the practical photo-thermo-sensitive genic male sterile line GD-1S in Guangdong that forms through screening system;
2), rice matter strong superior restorer line bright extensive 63 and plant type is good, recover recovery system " 3550 " hybridization that spectrum is wide, restoring force is strong preferably with coordinate force, breed intermediate materials and reestablish diplomatic relations with high-quality, high blast resisting strain R836-1 again, through many generations directedly select to breed with test cross in pairs that plant type is good, high-quality, disease-resistant recovery system wide extensive 122;
3) be female parent with photo-thermo-sensitive genic male sterile line GD-1S, recovering system wide extensive 122 with high-quality is male parent preparation super hybridized rice combination " Guangdong assorted 122 ".
Described maternal GD-1S originates and has following feature:
1) GD-1S is to be the photoperiod-temperature sensitive genie male-sterile line genetic donor with W7415S, with the special blue or green hybridization of high yield, disease-resistant conventional variety, and the Guangdong practicality photo-thermo-sensitive genic male sterile line GD-1S that forms through the shuttle breeding method systematic breeding of making in 11 years 18.
2) early broadcast the beginning fate season in Guangzhou be 89 days to GD-1S, and broadcast 65 days phases beginning season, plant neat and consistent, the stem number of blade 12.5-13.5 leaf evening; Plant height is 68-81cm, sword-like leave directly, wide slightly, leaf look pale green, grain is grown up, the bran point is colourless, tip of the spike grain has short awns, the leaf sheath pale green; Spike length is big, and the total grain husk of every fringe spends number to reach about 150; Thousand kernel weight 25-26 gram, the long 6.80mm of the grain of rice, the wide 2.14mm of grain, length and width ratio reaches 3.18; The white rate 9% of chalk, transparency 0.5, amylose content is 25%, glue denseness 97mm, protein content are 10.1%.
3) critical temperature of male sterility of GD-1S is 23.5 ± 0.5 ℃, and fertility conversion critical light temperature combined index is: 13.5h/23 ± 0.5 ℃, 12.5h/23.5 ± 0.5 ℃ and 11.5h/26.5 ± 0.5 ℃; The flowering habit of GD-1S is all better, stigma exposing ratio higher (75.5%), be white in color, grow up, vigor is stronger, coordinate force is good; High blast resisting (the Quan Qunkang ratio is 90.7%), middle bacterial blight-resisting (to bacterial leaf-blight IV type bacterium resistance is 3.5 grades, is 7 grades to V-type bacterium resistance); GD-1S is all two-line sterile lines preferably of a plant type, resistance, quality, outcrossing rate.
Described male parent wide extensive 122 is originated and is had following feature:
With conventional rice variety blue or green four short and international paddy rice to be recovered be IR54 hybridization, by artificial orientation's systematic breeding bred good, the anti-fertilizer of plant type disease-resistant, recover spectrum extensively, recovery that restoring force is strong is " 3550 ".Series of combination by its preparation has reached 240,000 hm in the production large scale application at present
2But it is few to recover system's " 3550 " pollen amount, and hybrid seed yield is low, and breeding time is long, and the assembly combination has weakly sensitivity, and these shortcomings have limited further developing and utilizing of it to a certain extent.And bright extensive 63 restoring forces are strong, and pollen amount is big, and adaptability is good, but anti-fertility is poor, and rice matter is general.In order to learn from other's strong points to offset one's weaknesses, " 3550 " are improved, promptly select for use bright extensive 63 and " 3550 " hybridization, breed in the middle of breeding material, and then reestablish diplomatic relations with high-quality, high blast resisting strain R836-1, directedly select to breed the good high-quality of plant type, disease-resistant, new restorer line wide extensive 122 that kind property is stable through many generations.
GD-1S closes the feature in " Guangdong assorted 122 " with wide extensive 122 combos:
In the 125 days time of infertility of Guangdong assorted 122 seasons plantation early, season in evening is 112 days, and comparison is according to precocious 4-5 days of Shanyou 63, early stage early growth and quick tiller, the middle and later periods cauline leaf is straight and upright, and is collecting and distributing moderate, Ye Xiahe; Tillering ability is medium, percentage of earbearing tiller height, mu number of productive ear 18.15 ten thousand; 105.2 centimetres of plant heights, 27.3 centimetres of spike lengths, 192 of every total grain panicles, ripening rate 82.5%, thousand kernel weight 27.1 grams, the grain of rice is elongated, and transparency is better.The artificial infection qualification result, blast resisting (the Quan Qunkang ratio is 85%), middle sense bacterial leaf-blight (5 grades of resistance general comments).
Adopt the present invention to have following outstanding effect:
(1) application of good photo-thermo-sensitive genic male sterile line: this invention be with in ripe, a long grain high-quality, disease-resistant, outcrossing seed-setting rate is high, breeding cost is low, the interaction by light and temperature type paddy rice caryon sterile line GD-1S that is adapted at production of hybrid seeds application under the light temperature ecological condition of south China is a female parent, with high-quality, disease-resistant, recovery that plant type is good system wide extensive 122 is male parent, the hybridization combo produce have the super high-yielding potentiality in ripe two be super hybridized rice combination Guangdong assorted 122.
(2) the novel strain type characteristic of tool super high-yielding potentiality: maternal GD-1S and the male parent extensively extensive 122 super hybridized rice Guangdong that make assorted 122 have living fast length morning, develop soon in the early stage leaf area of fertility, and blade drapes over one's shoulders, and the leaf area that absorbs luminous energy is big, the utility rate of luminous energy height; Growing the middle and later periods, strain leaf straight elevator, plant type is loose at first but afterwards tight, keeps ventilation and penetrating light, and colony's absorbing luminous energy is highly advantageous to.For its super high-yielding potentiality have been laid solid material base.This is different from the training less earlier growth of leaf area that is combined in that short 64S prepared also is kept upright, and the absorption luminous energy area of colony is less, can not make full use of the luminous energy that shines the field.
(3) adapt to the south China rice workspace: the super hybridized rice Guangdong that this project technology is made is medium assorted 122 breeding times, being adapted at South China's season plantation sooner or later uses, domestic existing two is that the super high-yielding combination is longer most breeding times, is difficult in the South China two seasons plantation sooner or later and uses.
(4) hybrid seed yield is expensive low: this invention is to utilize the GD-1S preparation Super Hybrid rice that stigma exposing ratio height, outcrossing are good, responsive to " 920 ", do not infect smut, have seed production and high-yield stable yields, " 920 " usage amount few, need not prevent and treat advantages such as smut, breeding cost are low.
(5) output reaches the super hybridized rice index: the super hybridized rice Guangdong assorted 122 that utilizes this project technology to be made, harvest index is up to 0.58, and continuous 2 years the evening season hundred mu of contiguous plants realized 11.25 tons/hectare of absolute yields, the super high-yielding index that the daily output is 100 kilograms/hectare: season in evening in 2000, hundred mu of contiguous plants cut examination with reality, be 112 days the time of infertility, output is 11.41 tons/hectare, has a daily output of 101.87 kilograms/hectare; Season evening calendar year 2001, hundred mu of contiguous plants cut examination with real, and be 111 days the time of infertility, and output reaches 11.62 tons/hectare, has a daily output of 104.67 kilograms of hectares.No matter be that the gross output or the daily output have all reached the super hybridized rice index that country is proposed.Simultaneously, this combination rice matter reaches 2 grades, blast resisting.
Four. the drawing explanation
Fig. 1 is a concrete breeding line spectrogram of the present invention.
Five. preferred embodiment
Specific implementation method of the present invention is described in detail in detail below.
It is female parent that present embodiment adopts photo-thermo-sensitive genic male sterile line GD-1S, recover system wide extensive 122 with high-quality is that male parent preparation super hybridized rice makes up " Guangdong assorted 122 ".Not only restorability is good for maternal GD-1S, and coordinate force is strong, and thousand kernel weight is bigger, and particle shape is good, and anti-pest are stronger, and column cap grows up, exposing ratio higher (75.5%), and vitality of stigma is strong, and flowering habit is good, the outcrossing seed-setting rate height, responsive to " 920 ", the hybrid seed yield height, cost is low.Wide extensive 122 tillering abilities of male parent are strong, and effectively fringe is many, and pollen amount is big, and the production of hybrid seeds is easy, and this combination hybrid seed yield can reach 150 kilograms.Concrete seeding technique main points and step are:
1) select to isolate production of hybrid seeds base preferably: around the seed farm, can not have other paddy pollen to seal in, isolated area should be in 100 meters, and in 200 meters of the isolated areas, all can not there be other Rice Production fields rice cultivation simultaneously in air port in this way;
2) select safe sensitive periods and heading stage: the weather conditions and the characteristics that at first will take into full account production of hybrid seeds production base, the sensitive periods (ear differentiation 3-6 phase) of guaranteeing photo-thermo-sensitive genic male sterile line GD-1S is to pass through under the light temperature ecological condition of illumination more than 12 hours, more than daily mean temperature 23.5 degree, and is stable and thoroughly sterile to guarantee GD-1S.In the numerous production of hybrid seeds in Sanya, Hainan south, GD-1S should sow about day at 10-15 in February, heading at the beginning of 5 months by the end of April, and pass through the April that sensitive periods, (〉=12 hours) were grown in higher and illumination in temperature; In the North Guangdong, the south production of hybrid seeds in early autumn of Gui Zhongbei or Hunan, should be arranged in by the end of August heading stage or at the beginning of 9 months, sensitive periods was passed through in the August of the long illumination of high temperature;
3) rationally arrange to broadcast the poor phase: in the numerous production of hybrid seeds in south of Sanya, Hainan, GD-1S should be than wide extensive 122 late sowings of first phase male parent about 8 days, than sowing in the Zao 2-3 of second phase male parent days; In continent early autumn system, maternal GD-1S generally than sowing in the slow 6-7 of first phase male parent days, broadcast than the Zao 1-2 of second phase male parent days;
4) seedling is strengthened in cultivation, throwing is in good time inserted: owing to father and mother's response to temperature originally is stronger, the long fringe early that easily causes of the length of time rice seedlings grow influences hybrid seed yield, so must in good time throwing insert.The length of time rice seedlings grow of general rice transplanting was advisable for 20-25 days; The length of time rice seedlings grow of rice transplanting with about 12 days for well;
5) rational row ratio and transplanting density: father and mother's one's own profession is advisable than with 2: 14 to 2: 16; Male parent transplanting specification is 33.3cm * 16.7cm, and the 3-4 seedling is inserted in every cave, 1 phase and the transplanting of staggering triangular in shape of 2 phase male parents; Maternal transplanting specification is 13.2cm * 13.2cm, and the 2-3 seedling is inserted in every cave; Reserve the work row about 26.4cm between father and mother's basis;
6) timely and appropriate discovery sprays " 920 ": because GD-1S is comparatively responsive to " 920 ", generally begin spray " 920 ", sprayed in continuous 2 days at female parent heading 10-15%, and first day spray 6 gram/mu, second day spray 8-9 gram/mu, the total consumption 14-15 gram of mu gets final product;
7) artificial auxiliary being pollinated: female parent reaches and begins when blooming the top to catch up with powder once with bamboo pole or rope, catches up with once every about 20 minutes later on, catches up with powder 3 times in 1 day;
8) perform diseases and pests controlling: put prevention first, the medicine of having resprayed 3-4 time in lifelong health care, promptly sprays the medicine of tillering after the rice transplanting about 15 days; The Sheng of tillering phase spray Sheng phase medicine; Cut foliar spray " 920 " and spray the booting medicine the previous day.
Purification and the multiplication technique of long-grained nonglutinous rice photo-thermo-sensitive genic male sterile line GD-1S:
In order to prevent male sterile line GD-1S along with the increasing of reproductive order of generation, the origin temp of its fertility conversion constantly raises, and brings very big risk to production of hybrid seeds production.For this reason, male sterile line must be protected pure numerous with expansion in strict accordance with certain rules.At first, light temperature ecological condition according to the South China, determine the light that faces that meets the photo-thermo-sensitive genic male sterile line fertility conversion of using and grow (12 hours) and critical temperature (23.5 ℃) in South China's production of hybrid seeds, by handling under the critical day set at climatic cabinate and the critical temperature condition, filter out satisfactory thorough sterile strain and cut stubble regeneration, regeneration breeding under the cold water irrigation condition, acquisition core kind-→ by the plantation of strain system, further identify under field conditions (factors) fertility and agronomic and economic traits and coordinate force-→ the strain system selecting to meet target cuts stubble regeneration, with cold water irrigation regeneration breeding breeder's stock-→ breeder's stock sowing cultivation, the cold water irrigation breeding obtain original seed-→ expand numerous produce the production that meets production of hybrid seeds requirement with kind.
Analyze according to Ministry of Agriculture's rice and quality of item supervision and inspection center, the Guangdong 122 brown rice rates 83.8% of mixing, polished rice rate 76.6%, head rice rate are 61.3%, grain length 7.0mm, length/width is than 3.2, transparency is 2 grades, and the alkali value that disappears is 4.8 grades, and the glue denseness reaches 94mm, amylose content is 23.4%, and protein content is 10.3%.Its exterior quality was 2 grades through the detection of Guangdong Province's grain and oil quality supervision measuring station in 1999.Rice is softer, good palatability.
Claims (3)
1. the breeding method of a super hybridized rice is characterized in that: the seed selection plant type is good, coordinate force is strong, origin temp low (≤24 ℃), the stable sterile phase is long, outcrossing seed-setting rate is high, the quality indica photo-thermo-sensitive genic male sterile line that is fit to South China's light temperature ecological condition is female parent; The quality indica superior restorer line is a male parent, is mixed with the super hybridized rice combination, is specifically realized by following steps:
1) the photosensitive-sensitive sterile line W7415S that does to be bred with academy of agricultural sciences, Hubei grain is the sterile gene donor,, high yield good, the special blue or green hybridization of high light efficiency conventional rice kind with the Guangdong plant type, by the shuttle method of breeding of Guangzhou and strange land, Hainan, cultivate the practical photo-thermo-sensitive genic male sterile line GD-1S in Guangdong that forms through screening system;
2), rice matter strong superior restorer line bright extensive 63 and plant type is good, recover recovery system " 3550 " hybridization that spectrum is wide, restoring force is strong preferably with coordinate force, breed intermediate materials and reestablish diplomatic relations with high-quality, high blast resisting strain R836-1 again, through many generations directedly select to breed with test cross in pairs that plant type is good, high-quality, disease-resistant recovery system wide extensive 122;
3) be female parent with photo-thermo-sensitive genic male sterile line GD-1S, recovering system wide extensive 122 with high-quality is male parent preparation super hybridized rice combination " Guangdong assorted 122 ".
2. the breeding method of a kind of super hybridized rice as claimed in claim 1, it is characterized in that: adopting photo-thermo-sensitive genic male sterile line GD-1S is that male parent preparation super hybridized rice makes up " Guangdong mixes 122 " for female parent, with high-quality recovery system wide extensive 122, and concrete seeding technique main points and step are:
1) select to isolate production of hybrid seeds base preferably: around the seed farm, can not have other paddy pollen to seal in, isolated area should be in 100 meters, and in 200 meters of the isolated areas, all can not there be other Rice Production fields rice cultivation simultaneously in air port in this way;
2) select safe sensitive periods and heading stage: the weather conditions and the characteristics that at first will take into full account production of hybrid seeds production base, the sensitive periods (ear differentiation 3-6 phase) of guaranteeing photo-thermo-sensitive genic male sterile line GD-1S is to pass through under the light temperature ecological condition of illumination more than 12 hours, more than daily mean temperature 23.5 degree, and is stable and thoroughly sterile to guarantee GD-1S.In the numerous production of hybrid seeds in Sanya, Hainan south, GD-1S should sow about day at 10-15 in February, heading at the beginning of 5 months by the end of April, and pass through the April that sensitive periods, (〉=12 hours) were grown in higher and illumination in temperature; In the North Guangdong, the south production of hybrid seeds in early autumn of Gui Zhongbei or Hunan, should be arranged in by the end of August heading stage or at the beginning of 9 months, sensitive periods was passed through in the August of the long illumination of high temperature;
3) rationally arrange to broadcast the poor phase: in the numerous production of hybrid seeds in south of Sanya, Hainan, GD-1S should be than wide extensive 122 late sowings of first phase male parent about 8 days, than sowing in the Zao 2-3 of second phase male parent days; In continent early autumn system, maternal GD-1S generally than sowing in the slow 6-7 of first phase male parent days, broadcast than the Zao 1-2 of second phase male parent days;
4) seedling is strengthened in cultivation, throwing is in good time inserted: owing to father and mother's response to temperature originally is stronger, the long fringe early that easily causes of the length of time rice seedlings grow influences hybrid seed yield, so must in good time throwing insert.The length of time rice seedlings grow of general rice transplanting was advisable for 20-25 days; The length of time rice seedlings grow of rice transplanting with about 12 days for well;
5) rational row ratio and transplanting density: father and mother's one's own profession is advisable than with 2: 14 to 2: 16; Male parent transplanting specification is 33.3cm * 16.7cm, and the 3-4 seedling is inserted in every cave, 1 phase and the transplanting of staggering triangular in shape of 2 phase male parents; Maternal transplanting specification is 13.2cm * 13.2cm, and the 2-3 seedling is inserted in every cave; Reserve the work row about 26.4cm between father and mother's basis;
6) timely and appropriate discovery sprays " 920 ": because GD-1S is comparatively responsive to " 920 ", generally begin spray " 920 ", sprayed in continuous 2 days at female parent heading 10-15%, and first day spray 6 gram/mu, second day spray 8-9 gram/mu, the total consumption 14-15 gram of mu gets final product;
7) artificial auxiliary being pollinated: female parent reaches and begins when blooming the top to catch up with powder once with bamboo pole or rope, catches up with once every about 20 minutes later on, catches up with powder 3 times in 1 day;
8) perform diseases and pests controlling: put prevention first, the medicine of having resprayed 3-4 time in lifelong health care, promptly sprays the medicine of tillering after the rice transplanting about 15 days; The Sheng of tillering phase spray Sheng phase medicine; Cut foliar spray " 920 " and spray the booting medicine the previous day.
3. the breeding method of a kind of super hybridized rice as claimed in claim 1, it is characterized in that: it is numerous with expansion that long-grained nonglutinous rice photo-thermo-sensitive genic male sterile line GD-1S adopts following steps to purify: according to the light temperature ecological condition of South China, determine the light that faces that meets the photo-thermo-sensitive genic male sterile line fertility conversion of using and grow (12 hours) and critical temperature (23.5 ℃) in South China's production of hybrid seeds, by handling under the critical day set at climatic cabinate and the critical temperature condition, filter out satisfactory thorough sterile strain and cut stubble regeneration, regeneration breeding under the cold water irrigation condition, acquisition core kind-→ by the plantation of strain system, further identify under field conditions (factors) fertility and agronomic and economic traits and coordinate force-→ the strain system selecting to meet target cuts stubble regeneration, with cold water irrigation regeneration breeding breeder's stock-→ breeder's stock sowing cultivation, the cold water irrigation breeding obtain original seed-→ expand numerous produce the production that meets production of hybrid seeds requirement with kind.
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| CN102771383A (en) * | 2012-08-13 | 2012-11-14 | 江苏丰源种业有限公司 | Method for breeding and seed production of superior multi-resistance high-yield two-line hybrid rice |
| CN104770291A (en) * | 2015-04-20 | 2015-07-15 | 中国水稻研究所 | Selective breeding method for two-line paddy sterility line with low sterile critical temperature |
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