CN110089366A - A kind of canopy room gets over summer method for cultivating tomato - Google Patents
A kind of canopy room gets over summer method for cultivating tomato Download PDFInfo
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- CN110089366A CN110089366A CN201910516568.1A CN201910516568A CN110089366A CN 110089366 A CN110089366 A CN 110089366A CN 201910516568 A CN201910516568 A CN 201910516568A CN 110089366 A CN110089366 A CN 110089366A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/30—Grafting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/05—Fruit crops, e.g. strawberries, tomatoes or cucumbers
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/14—Greenhouses
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/24—Devices or systems for heating, ventilating, regulating temperature, illuminating, or watering, in greenhouses, forcing-frames, or the like
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/24—Devices or systems for heating, ventilating, regulating temperature, illuminating, or watering, in greenhouses, forcing-frames, or the like
- A01G9/246—Air-conditioning systems
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/25—Greenhouse technology, e.g. cooling systems therefor
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Abstract
It the invention belongs to technique for cultivating tomatoes field, discloses a kind of canopy room and gets over summer method for cultivating tomato, carry out the healthy and strong stock of variety selection, culture, scion, grafting, managing after grafting;Young plant after connecting closed shading be placed in plastic greenhouse, it is watered with water, keeps 95% or more air humidity, 23-28 DEG C of temperature daytime, 15-22 DEG C of night, second day opening plastic foil, air humidity is reduced in 76%-80% after 3 days, and the 4th day 0.5-1 hours light-exposed, 5th day 1-2 hours light-exposed, 6th day 4-5 hours light-exposed, and beginning whole day is light-exposed within the 7th day, and unlace transplanting in time after wound healing in 7-8 days.The present invention carries out disinfection processing prior to seeding, can kill germ entrained by seed, the generation of pre- anti-virus improves the survival rate of seed;It is grafted below stock cotyledon, and will be set as trapezoidal at grafting, contact area is big, high survival rate.
Description
Technical field
The invention belongs to technique for cultivating tomatoes fields more particularly to a kind of canopy room to get over summer method for cultivating tomato.
Background technique
Tomato, for scientific name tomato, it had not only contained rich and varied nutrition, but also had the shape of beautiful charming tomato;
It is both good taste and fruit Sino-U.S. product in dish, and there are many functions, the referred to as fruit in magical dish.Tomato is used to solution of relieving summer heat
It can match in excellence or beauty yearningly with watermelon.However, ascorbic content is but about as much as 10 times of watermelon in tomato.And due to having
The protection of machine acid, in storage and gastronomical process, the vitamin C contained by it is not subject to destroy again, and human body utilization rate is very high.Cause
This, often eats tomato, for treatment scurvy, anaphylactoid purpura, flu and promotes wound healing, there is important role.West
Red persimmon sugar content is also very high, about 1.5%-4.5%, and wherein most be the glucose that is easy to be directly absorbed by the body and
Fructose.Tomato carrotene also rich in, vitamin B complex have including to protection vascular health, prevention and treatment hypertension
The rutin of certain effect.The content of various vitamins is 2-4 times higher than apple, pears, banana, grape, it, which contains, accounts for its total weight
0.6% various minerals, wherein more with calcium, phosphorus, zinc, iron take second place, and in addition there are manganese, copper, iodine etc. to want microelement.These
Minerals, it is particularly advantageous to baby and upgrowth and development of children.However, during existing cultivating tomato, after grafting, planting percent is not
It is too high, and the quality of seedling is also bad, is easy virus infection disease, has aggravated the burden of peasant, seriously constrained the big rule of tomato
Mould production;And booth cultivation defence disease pest effect is poor;Meanwhile to tomato by manual grading skill, low efficiency is at high cost, long
The classification process of time is unfavorable to the circulation of fresh fruit of vegetables;Low precision can only lean on personal experience to be classified target, cannot be to target
Quantitative description, causes classification results to vary with each individual;Large labor intensity, manual grading skill are a kind of monotonous works, are easy
Visual fatigue is generated, effectiveness of classification is influenced.
In conclusion problem of the existing technology is:
During existing cultivating tomato, planting percent is less high after grafting, and the quality of seedling is also bad, is easy infection disease
Viral disease, has aggravated the burden of peasant, seriously constrains tomato large-scale production;And booth cultivation defence disease pest effect is poor.
Summary of the invention
In view of the problems of the existing technology, the present invention provides a kind of canopy rooms to get over summer method for cultivating tomato.
The invention is realized in this way a kind of canopy room gets over summer method for cultivating tomato includes:
(1) variety selection
The wild breeding tomato kind of the high resistance to bacterial wilt of Root-stock selection and root knot nematode disease, by 40 DEG C of temperature of seed before sowing
Water impregnates 3.5-4 hours, places into 1% liquor potassic permanganate and impregnates 15-20 minutes, takes out cleaning vernalization;
(2) the healthy and strong stock of culture, scion
Stock shifts to an earlier date 6-7 days and sows, and in sterile soil, scion uses flat furrow light seeding for stock program request, will connect within 1-2 days before grafting
Fringe temporary planting is into the nutritive cube equipped with plant production nutrient solution;
(3) it grafts
When stock grows to 9-l0cm high, stem thickness 5-6mm grafting is crosscutting with blade at 1.1-1.5cm below the stock cotyledon
Stock stem is removed top, then is cut off by oblique among stem, and 45° angle is presented in tangent line and axis, it is desirable that section is smooth, then will
Scion retains 2 to 3 true leaves, cuts away lower part with blade, top cutting section is whittled into trapezoidal, trapezoidal size, gradient is answered
It is suitable with stock notch, with scion is inserted into stock, with the tight bondage of belt, it is intimately associated stock, scion, outside is used
Plastic foil, which packs tightly, ensures interface humidity and healing condition;
(4) managing after grafting
Young plant after connecting closed shading be placed in plastic greenhouse, be watered with water, keep 95% or more air humidity, temperature
On 23-28 DEG C of daytime, 15-22 DEG C of night, second day opening plastic foil, air humidity is reduced in 76%-80% after 3 days, the
Four days 0.5-1 hours light-exposed, and the 5th day 1-2 hours light-exposed, and the 6th day 4-5 hours light-exposed, and beginning whole day is light-exposed within the 7th day, 7-8
Unlace transplanting in time after its wound healing.
Further, it further comprises disease pest prevention that the canopy room, which gets over summer method for cultivating tomato, is specifically included:
Summer high-temperature season digs a plurality of ditch in canopy room, is trained between ditch and ditch into ridge, the planting site of entire canopy room with soil
The difference in height of the arrangement at face formation furrow interval, ridge and ditch is 25-40 centimetres;
In the ditch, it is put into the water with the encapsulation of not thermal barrier, water is more than 50% of total water capacity in ditch;
The original film of canopy room is tight-closed, it cleans up, transparent heat insulating material is covered in canopy.
Further, in managing after grafting, by adjusting sunshade net sunshading and temperature reducing, kept for 25-28 DEG C of temperature daytime, night temperature
15-18 DEG C of degree.
Further, it before cultivating healthy and strong stock, scion, also needs to carry out: acquiring the tender stem of healthy and strong no disease and pests harm tomato
Section, as the explant of tissue cultures, collected explant is cleaned up with washing powder water, then be placed under flowing water and rinse
30min with 75% alcohol disinfecting 10-60s on superclean bench, then with aseptic water washing 3-5 times, uses mass percent later
For 0.1% mercuric chloride sterilize 3-10min, aseptic water washing 3-5 times;
Explant after disinfection is inoculated into induced medium, culturing room is sent into and carries out sterile culture, culture medium MS+
6-BA0.6mg/L+NAA0.3mg/L+ sucrose 30000mg/L+ agar 10000-15000mg/L;After induction differentiation, divided
Change seedling, differentiation seedling is transferred in proliferated culture medium and carries out Multiplying culture, obtains largely breaking up seedling seedling, proliferated culture medium MS
+ 6-BA1.0mg/L+NAA0.3mg/L+ sucrose 30000mg/L+ agar 10000-15000mg/L;
Differentiation seedling seedling inoculation is subjected to culture of rootage, root media 1/2MS+ in root media
IBA0.5mg/L+NAA0.1mg/L+ sucrose 30000mg/L+ agar 10000-15000mg/L, dark culture 8-14d, then illumination training
It supports 3 weeks, the tissue-cultured seedling taken root;
Training tissue culture seedling 3-5d after taking root is transplanted in the dish for cultivating that vermiculite is matrix and takes exercise in arched shed, works as group
Training transplantation of seedlings survives rear 40-60d, is transplanted to big Tanaka.
Further, the working solution of MS culture medium includes:
A great number of elements, including ammonium nitrate NH2NO3, potassium nitrate KNO3;
Microelement, including potassium iodide KI, boric acid H3BO3, manganese sulfate MnSO4·4H2O, zinc sulfate ZnSO4·7H2O;
Organic solution, including niacin, puridoxine hydrochloride;
Molysite, including FeSO4·7H2O。
Further, managing after grafting further comprises: soil management: plough deeply, apply fertilizer and do furrow, ploughed deeply before field planting 30cm~
40cm, apply per acre 4 ton/mu~5 ton of decomposed manure/mu, 50 kgs/acre~70 kgs/acre of general calcium;Furrow face be made into 60cm~
80cm wide, high 20cm~30cm, furrow width 80cm~100cm between furrow and furrow.
Further, in grafting, further comprise:
Field planting: before rudiment, ditch depth 25cm~30cm, the decomposed thin fertilizer of ditch bottom application, and puddles with soil, 1 plant/ditch~2 plant/
Ditch, average every plant of dose are not less than 2 kilograms, with line-spacing 100cm~150cm, spacing in the rows 50cm~80cm, and 600 plants/acre~700
Plant/acre field planting;
Trimming: according to the thickness of branch, sturdy branch stays length, slightly stays shearing in the middle part of long, medium branch compared with vigorous branches.
Further, the prevention and control of plant diseases, pest control prevention and control of plant diseases, pest control: is carried out using cultural control, physical control and spray liquor.
Further, the method for cultural control are as follows: select resistance strong, adapt to the kind of local production, by dense planting and repair
The ventilation and penetrating light for improving field plant is cut, the generation of pest and disease damage is reduced, is enhanced field management, weeds is removed, destroys plant diseases and insect pests
Habitat;
The method of physical control are as follows: using the taxis of pest, carry out light trap, colour table trapping or anisotropic trapping, use
Black light lamp traps and kills chafer and lepidoptera imago, installs yellow card's trap winged aphids;
Spray liquor method are as follows: prepare Bordeaux's liquor of l:1:200,50,000 grams/acre~60 kilograms of spray liquor dosage/
Mu.
Further, seedling 1000 times of liquid~1200 times liquid of thiophanate methyl+1200 times of gamma cyhalothrin before being colonized
Liquid impregnates 10 minutes~15 minutes, dries wait be colonized;
Mu 1 kilogram~1.5 kilograms of 50% phoxim emulsifiable concentrate or 1 kilogram~1.5 kilograms of 5% chlopyrifos granule when field planting
It mixes 10 kilograms~20 kilograms sawmilling to spread fertilizer over the fields in seedling root, and backfills soil, kill or prevent subterranean pest-insect;
Field planting pour within preceding 3 days~7 days it is once permeable, poured after field planting 1 time~2 times it is permeable, and cover black film.
Advantages of the present invention and good effect are as follows: the present invention carries out disinfection processing prior to seeding, can kill seed and be taken
The germ of band, the generation of pre- anti-virus improve the survival rate of seed;It grafts, and will be set as at grafting below stock cotyledon
Trapezoidal, contact area is big, high survival rate, and cutting face is smooth, smooth, and the interface of scion and stock is closer, does not stitch theoretically
Gap improves survival rate so that wound be made to be easier to heal;Made by disease pest prevention module without biology in arable layer, meanwhile, water
Slowly seep it is lower can wash away part fertilizer and pesticide residue, mitigate the soil salinization, for produce Safe Agricultural Product lay it is good
Basis;Solar energy can more be converted into thermal energy after entering canopy room transformation, and more be deposited in canopy room in soil, thus greatly
Amplitude improves canopy indoor air temperature and ground temperature, the more effectively germ and pest of elimination air, canopy body surface face, deep soil.
The method of the present invention improves the breeding coefficient of tomato, compensates for some shortcomings of traditional reproduction technique, such as reduces
The pollution rate of explant, the breeding coefficient for improving induction differentiation seedling and shoot proliferation, transplanting survival rate also greatly improve, increase
Add tomato reproductive number, accelerates reproduction speed.Explant acquisition, Disinfection Effect, superclean bench switching effect, sterile
Culturing room's culture effect, rooting induction effect, transplantation of seedlings effect of taking root, seedling expanding propagation speed etc. are superior to other tomatoes
Reproduction technique, the scale established, batch production, standardization nursery stable system, it is with short production cycle, accelerate the numerous of tomato
Speed is grown, the limitation in the season, kind property of breeding is broken.Tissue culture formula of the invention is best group of a large amount of expanding propagations of tomato
Formula is closed, tomato plants can be bred with rapid, high volume, meet the market demand.
Detailed description of the invention
Fig. 1 is that canopy room provided in an embodiment of the present invention gets over summer method for cultivating tomato flow chart.
Specific embodiment
In order to further understand the content, features and effects of the present invention, the following examples are hereby given, and cooperate attached drawing
Detailed description are as follows.
Structure of the invention is explained in detail with reference to the accompanying drawing.
As shown in Figure 1, canopy room provided in an embodiment of the present invention get over summer method for cultivating tomato the following steps are included:
S101: variety selection: the wild breeding tomato kind of the high resistance to bacterial wilt of Root-stock selection and root knot nematode disease, before sowing
40 DEG C of warm water of seed are impregnated 3.5-4 hours, places into 1% liquor potassic permanganate and impregnates 15-20 minutes, take out cleaning
Vernalization.
S102: cultivate healthy and strong stock, scion: stock shifts to an earlier date 6-7 days and sows, and in sterile soil, scion is adopted for stock program request
With flat furrow light seeding, scion was planted into the nutritive cube equipped with plant production nutrient solution in 1-2 days before grafting.
S103: grafting: when stock grows to 9-l0cm high, stem thickness 5-6mm is grafted, below the stock cotyledon at 1.1-1.5cm
With the crosscutting stock stem of blade, remove top, then cut off by oblique among stem, 45° angle is presented in tangent line and axis, it is desirable that section is flat
It is sliding, scion is then retained into upper piece 2 to 3 true leaves, cuts away lower part with blade, top cutting section is whittled into trapezoidal, trapezoidal is big
Small, gradient should be suitable with stock notch, with scion is inserted into stock, with the tight bondage of belt, ties stock, scion closely
It closes, outside is packed tightly with plastic foil ensures interface humidity and healing condition.
S104: managing after grafting: young plant after connecting closed shading be placed in plastic greenhouse, be watered with water, keep 95% with
On air humidity, on 23-28 DEG C of temperature daytime, 15-22 DEG C of night, second day opening plastic foil, air humidity reduces after 3 days
To in 76%-80%, the 4th day is 0.5-1 hours light-exposed, and the 5th day is 1-2 hours light-exposed, and the 6th day is 4-5 hours light-exposed, and the 7th day
Beginning whole day is light-exposed, and unlace transplanting in time after wound healing in 7-8 days.
As the preferred embodiment of the present invention, it further comprises disease pest prevention that canopy room, which gets over summer method for cultivating tomato, specific to wrap
It includes:
Summer high-temperature season digs a plurality of ditch in canopy room, is trained between ditch and ditch into ridge, the planting site of entire canopy room with soil
The difference in height of the arrangement at face formation furrow interval, ridge and ditch is 25-40 centimetres.
In the ditch, it is put into the water with the encapsulation of not thermal barrier, water is more than 50% of total water capacity in ditch.
The original film of canopy room is tight-closed, it cleans up, transparent heat insulating material is covered in canopy.
As the preferred embodiment of the present invention, in managing after grafting, passes through and adjust sunshade net sunshading and temperature reducing, keep temperature daytime
25-28 DEG C, 15-18 DEG C of nocturnal temperature.
Further, it before cultivating healthy and strong stock, scion, also needs to carry out: acquiring the tender stem of healthy and strong no disease and pests harm tomato
Section, as the explant of tissue cultures, collected explant is cleaned up with washing powder water, then be placed under flowing water and rinse
30min with 75% alcohol disinfecting 10-60s on superclean bench, then with aseptic water washing 3-5 times, uses mass percent later
For 0.1% mercuric chloride sterilize 3-10min, aseptic water washing 3-5 times;
Explant after disinfection is inoculated into induced medium, culturing room is sent into and carries out sterile culture, culture medium MS+
6-BA0.6mg/L+NAA0.3mg/L+ sucrose 30000mg/L+ agar 10000-15000mg/L;After induction differentiation, divided
Change seedling, differentiation seedling is transferred in proliferated culture medium and carries out Multiplying culture, obtains largely breaking up seedling seedling, proliferated culture medium MS
+ 6-BA1.0mg/L+NAA0.3mg/L+ sucrose 30000mg/L+ agar 10000-15000mg/L.
Differentiation seedling seedling inoculation is subjected to culture of rootage, root media 1/2MS+ in root media
IBA0.5mg/L+NAA0.1mg/L+ sucrose 30000mg/L+ agar 10000-15000mg/L, dark culture 8-14d, then illumination training
It supports 3 weeks, the tissue-cultured seedling taken root.
Training tissue culture seedling 3-5d after taking root is transplanted in the dish for cultivating that vermiculite is matrix and takes exercise in arched shed, works as group
Training transplantation of seedlings survives rear 40-60d, is transplanted to big Tanaka.
As the preferred embodiment of the present invention, the working solution of MS culture medium includes:
A great number of elements, including ammonium nitrate NH2NO3, potassium nitrate KNO3。
Microelement, including potassium iodide KI, boric acid H3BO3, manganese sulfate MnSO4·4H2O, zinc sulfate ZnSO4·7H2O。
Organic solution, including niacin, puridoxine hydrochloride.
Molysite, including FeSO4·7H2O。
As the preferred embodiment of the present invention, managing after grafting further comprises: soil management: ploughing deeply, apply fertilizer and do furrow, fixed
30cm~40cm is ploughed deeply before plant, apply per acre 4 ton/mu~5 ton of decomposed manure/mu, 50 kgs/acre~70 kgs/acre of general calcium;Furrow
Face is made into 60cm~80cm wide, high 20cm~30cm, furrow width 80cm~100cm between furrow and furrow.
As the preferred embodiment of the present invention, in grafting, further comprise:
Field planting: before rudiment, ditch depth 25cm~30cm, the decomposed thin fertilizer of ditch bottom application, and puddles with soil, 1 plant/ditch~2 plant/
Ditch, average every plant of dose are not less than 2 kilograms, with line-spacing 100cm~150cm, spacing in the rows 50cm~80cm, and 600 plants/acre~700
Plant/acre field planting.
Trimming: according to the thickness of branch, sturdy branch stays length, slightly stays shearing in the middle part of long, medium branch compared with vigorous branches.
As the preferred embodiment of the present invention, disease the prevention and control of plant diseases, pest control: is carried out using cultural control, physical control and spray liquor
Pest control.
As the preferred embodiment of the present invention, the method for cultural control are as follows: select resistance strong, adapt to the product of local production
Kind, improve the ventilation and penetrating light of field plant by dense planting and trimming, reduce the generation of pest and disease damage, enhance field management, removes miscellaneous
Grass, the habitat to destroy plant diseases and insect pests;
The method of physical control are as follows: using the taxis of pest, carry out light trap, colour table trapping or anisotropic trapping, use
Black light lamp traps and kills chafer and lepidoptera imago, installs yellow card's trap winged aphids;
Spray liquor method are as follows: prepare Bordeaux's liquor of l:1:200,50,000 grams/acre~60 kilograms of spray liquor dosage/
Mu.
As the preferred embodiment of the present invention, seedling 1000 times of liquid~1200 times liquid of thiophanate methyl+efficient chlorine before being colonized
1200 times of liquid of flucythrinate impregnate 10 minutes~15 minutes, dry wait be colonized;
Mu 1 kilogram~1.5 kilograms of 50% phoxim emulsifiable concentrate or 1 kilogram~1.5 kilograms of 5% chlopyrifos granule when field planting
It mixes 10 kilograms~20 kilograms sawmilling to spread fertilizer over the fields in seedling root, and backfills soil, kill or prevent subterranean pest-insect;
Field planting pour within preceding 3 days~7 days it is once permeable, poured after field planting 1 time~2 times it is permeable, and cover black film.
Below with reference to effect, the invention will be further described.
The present invention carries out disinfection processing prior to seeding, can kill germ entrained by seed, the generation of pre- anti-virus mentions
The survival rate of high seed;It is grafted below stock cotyledon, and will be set as trapezoidal at grafting, contact area is big, and high survival rate is cut
Bevel is smooth, smooth, and the interface of scion and stock is closer, theoretically without gap, so that wound be made to be easier to heal, improves
Survival rate;Made by disease pest prevention module without biology in arable layer, meanwhile, water can wash away part fertilizer and agriculture under slowly seeping
Medicine residual mitigates the soil salinization, lays a good foundation for production Safe Agricultural Product;Solar energy can be more after entering canopy room transformation
It mostly is converted into thermal energy, and is more deposited in canopy room in soil, to increase substantially canopy indoor air temperature and ground temperature, is more had
The germ and pest for eliminating air, canopy body surface face, deep soil of effect.
The method of the present invention improves the breeding coefficient of tomato, compensates for some shortcomings of traditional reproduction technique, such as reduces
The pollution rate of explant, the breeding coefficient for improving induction differentiation seedling and shoot proliferation, transplanting survival rate also greatly improve, increase
Add tomato reproductive number, accelerates reproduction speed.Explant acquisition, Disinfection Effect, superclean bench switching effect, sterile
Culturing room's culture effect, rooting induction effect, transplantation of seedlings effect of taking root, seedling expanding propagation speed etc. are superior to other tomatoes
Reproduction technique, the scale established, batch production, standardization nursery stable system, it is with short production cycle, accelerate the numerous of tomato
Speed is grown, the limitation in the season, kind property of breeding is broken.Tissue culture formula of the invention is best group of a large amount of expanding propagations of tomato
Formula is closed, tomato plants can be bred with rapid, high volume, meet the market demand.
The above is only the preferred embodiments of the present invention, and is not intended to limit the present invention in any form,
Any simple modification made to the above embodiment according to the technical essence of the invention, equivalent variations and modification, belong to
In the range of technical solution of the present invention.
Claims (10)
1. a kind of canopy room gets over summer method for cultivating tomato, which is characterized in that the canopy room gets over summer method for cultivating tomato and includes:
(1) variety selection
The wild breeding tomato kind of the high resistance to bacterial wilt of Root-stock selection and root knot nematode disease soaks 40 DEG C of warm water of seed before sowing
Bubble 3.5-4 hours, places into 1% liquor potassic permanganate and impregnates 15-20 minutes, takes out cleaning vernalization;
(2) the healthy and strong stock of culture, scion
Stock shifts to an earlier date 6-7 days and sows, and in sterile soil, scion uses flat furrow light seeding for stock program request, and grafting first 1-2 days false by scion
It plants in the nutritive cube equipped with plant production nutrient solution;
(3) it grafts
When stock grows to 9-l0cm high, stem thickness 5-6mm grafting, with the crosscutting stock of blade at 1.1-1.5cm below the stock cotyledon
Stem removes top, then is cut off by oblique among stem, and 45° angle is presented in tangent line and axis, it is desirable that section is smooth, then by scion
Retain 2 to 3 true leaves, cut away lower part with blade, top cutting section is whittled into trapezoidal, trapezoidal size, gradient should be with anvil
The wooden notch is suitable, with scion is inserted into stock, with the tight bondage of belt, is intimately associated stock, scion, outside plastics
Film, which packs tightly, ensures interface humidity and healing condition;
(4) managing after grafting
Young plant after connecting closed shading be placed in plastic greenhouse, be watered with water, keep 95% or more air humidity, temperature daytime
23-28 DEG C, 15-22 DEG C of night, second day opening plastic foil, air humidity is reduced in 76%-80% after 3 days, and the 4th day
0.5-1 hours light-exposed, the 5th day 1-2 hours light-exposed, and the 6th day 4-5 hours light-exposed, and beginning whole day is light-exposed within the 7th day, hurts within 7-8 days
Unlace transplanting in time after mouth healing.
2. canopy room as described in claim 1 gets over summer method for cultivating tomato, which is characterized in that the canopy room gets over summer cultivating tomato
Method further comprises disease pest prevention, is specifically included:
Summer high-temperature season digs a plurality of ditch in canopy room, is trained between ditch and ditch with soil into ridge, the plantation ground shape of entire canopy room
At the arrangement at furrow interval, the difference in height of ridge and ditch is 25-40 centimetres;
In the ditch, it is put into the water with the encapsulation of not thermal barrier, water is more than 50% of total water capacity in ditch;
The original film of canopy room is tight-closed, it cleans up, transparent heat insulating material is covered in canopy.
3. canopy room as described in claim 1 gets over summer method for cultivating tomato, which is characterized in that in managing after grafting, pass through adjusting
Sunshade net sunshading and temperature reducing, daytime are kept for 25-28 DEG C of temperature, 15-18 DEG C of nocturnal temperature.
4. canopy room as described in claim 1 gets over summer method for cultivating tomato, which is characterized in that
It before cultivating healthy and strong stock, scion, also needs to carry out: the stem segments of healthy and strong no disease and pests harm tomato is acquired, as tissue
Collected explant is cleaned up with washing powder water, then is placed under flowing water and rinses 30min by the explant of culture, ultra-clean
With 75% alcohol disinfecting 10-60s on workbench, then with aseptic water washing 3-5 times, the liter for being later 0.1% with mass percent
Mercury sterilize 3-10min, aseptic water washing 3-5 times;
Explant after disinfection is inoculated into induced medium, culturing room is sent into and carries out sterile culture, culture medium MS+6-
BA0.6mg/L+NAA0.3mg/L+ sucrose 30000mg/L+ agar 10000-15000mg/L;After induction differentiation, broken up
Differentiation seedling is transferred in proliferated culture medium and carries out Multiplying culture, obtains largely breaking up seedling seedling, proliferated culture medium MS+ by seedling
6-BA1.0mg/L+NAA0.3mg/L+ sucrose 30000mg/L+ agar 10000-15000mg/L;
Differentiation seedling seedling inoculation is subjected to culture of rootage, root media 1/2MS+IBA0.5mg/L+ in root media
NAA0.1mg/L+ sucrose 30000mg/L+ agar 10000-15000mg/L, dark culture 8-14d, then illumination cultivation 3 weeks, it is pricked
The tissue-cultured seedling of root;
Training tissue culture seedling 3-5d after taking root is transplanted in the dish for cultivating that vermiculite is matrix and takes exercise in arched shed, works as tissue-cultured seedling
40-60d after transplant survival is transplanted to big Tanaka.
5. canopy room as claimed in claim 4 gets over summer method for cultivating tomato, which is characterized in that
The working solution of MS culture medium includes:
A great number of elements, including ammonium nitrate NH2NO3, potassium nitrate KNO3;
Microelement, including potassium iodide KI, boric acid H3BO3, manganese sulfate MnSO4·4H2O, zinc sulfate ZnSO4·7H2O;
Organic solution, including niacin, puridoxine hydrochloride;
Molysite, including FeSO4·7H2O。
6. canopy room as described in claim 1 gets over summer method for cultivating tomato, which is characterized in that managing after grafting further comprises:
Soil management: ploughing deeply, apply fertilizer and do furrow, 30cm~40cm is ploughed deeply before field planting, apply per acre 4 ton/mu~5 ton of decomposed manure/mu,
50 kgs/acre~70 kgs/acre of general calcium;Furrow face is made into 60cm~80cm wide, high 20cm~30cm, the furrow width 80cm between furrow and furrow
~100cm.
7. canopy room as described in claim 1 gets over summer method for cultivating tomato, which is characterized in that in grafting, further comprise:
Field planting: before rudiment, ditch depth 25cm~30cm, the decomposed thin fertilizer of ditch bottom application, and puddles with soil, 1 plant/ditch~2 plant/ditch,
Average every plant of dose is not less than 2 kilograms, with line-spacing 100cm~150cm, spacing in the rows 50cm~80cm, 600 plants/acre~700 plants/
Mu field planting;
Trimming: according to the thickness of branch, sturdy branch stays length, slightly stays shearing in the middle part of long, medium branch compared with vigorous branches.
8. canopy room as claimed in claim 2 gets over summer method for cultivating tomato, which is characterized in that the prevention and control of plant diseases, pest control: anti-using agricultural
It controls, physical control and spray liquor carry out the prevention and control of plant diseases, pest control.
9. canopy room as claimed in claim 8 gets over summer method for cultivating tomato, which is characterized in that the method for cultural control are as follows: select
Resistance is strong, adapts to the kind of local production, improves the ventilation and penetrating light of field plant by dense planting and trimming, reduces pest and disease damage
Occur, enhance field management, removes weeds, the habitat to destroy plant diseases and insect pests;
The method of physical control are as follows: using the taxis of pest, light trap, colour table trapping or anisotropic trapping are carried out, using black light
Lamp traps and kills chafer and lepidoptera imago, installs yellow card's trap winged aphids;
Spray liquor method are as follows: prepare Bordeaux's liquor of l:1:200,50,000 grams/acre~60,000 grams/acre of spray liquor dosage.
10. canopy room as claimed in claim 7 gets over summer method for cultivating tomato, which is characterized in that
Seedling is impregnated 10 minutes with 1000 times of liquid~1200 times liquid of thiophanate methyl+1200 times of gamma cyhalothrin liquid before being colonized
It~15 minutes, dries wait be colonized;
Mu mixes 10 with 1 kilogram~1.5 kilograms of 50% phoxim emulsifiable concentrate or 1 kilogram~1.5 kilograms of 5% chlopyrifos granule when field planting
Kilogram~20 kilograms of sawmilling spread fertilizer over the fields in seedling root, and backfill soil, it kills or prevention subterranean pest-insect;
Field planting pour within preceding 3 days~7 days it is once permeable, poured after field planting 1 time~2 times it is permeable, and cover black film.
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CN114946434A (en) * | 2022-07-05 | 2022-08-30 | 龙岩市农业科学研究所 | Grafting seedling method for tomato seedlings without cotyledon on rootstocks |
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