CN110074063B - Device and method for remotely observing and breeding corophium acherusicum - Google Patents
Device and method for remotely observing and breeding corophium acherusicum Download PDFInfo
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- CN110074063B CN110074063B CN201910363566.3A CN201910363566A CN110074063B CN 110074063 B CN110074063 B CN 110074063B CN 201910363566 A CN201910363566 A CN 201910363566A CN 110074063 B CN110074063 B CN 110074063B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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Abstract
The invention discloses a device and a method for remotely observing and breeding corophium acherusicum. The device comprises an insect raising cage, wherein a zipper door with the height smaller than that of the insect raising cage is arranged on the right side surface of the insect raising cage, a channel for collecting corophium and communicated with the interior of the insect raising cage is arranged in the middle of the left side surface of the insect raising cage and close to the lower position, a horn mouth with a small outside and a large inside is arranged in the middle of the collecting channel, the diameter of the large mouth of the horn mouth is the same as that of the collecting channel, rope drawing holes are formed in the positions of the large mouth of the collecting channel and the outlet position of the collecting channel, and a drawing rope is threaded through the rope drawing holes; an artificial nest made of PVC pipes is arranged in the insect-raising cage and at a position close to the intersection of the upper side and the front side, and the interior of each PVC pipe is filled with reed pipes; the inside and be close to collection passageway apex angle position department of insect feeding cage installs the camera. The invention solves the problems of difficult observation, low trapping efficiency and the like, and reduces the risk of being bitten when the adult bee is utilized for testing.
Description
Technical Field
The invention relates to a remote observation and breeding device for bees, in particular to an indoor remote observation and breeding device and method for hunting solitary wasp corophium acherusicum.
Background
Corophium acherum (Rhynchium quinquecintum) belongs to Hymenoptera, wasp superfamily (Vespiodea), and corophium acherum family (Eumenidae), is a foodstuff specially used for hunting solitary bees and capturing lepidoptera larvae as offspring. Has the characteristics of large capture amount and wide hunting range. The active period of the bee is 7-9 months, which is consistent with the emergence period of a plurality of important pests of Lepidoptera. Secondly, the adult bees have the habit of visiting flowers and are an important one of pollinating bees. The dual characteristics of predation and flower pollination enable corophium acherusicum to become a carrier species with two important ecological service functions of pest biological control and pollination of insects.
In the field, corophium acherusicum was nested in a hollow bamboo tube. When carrying out the research to potter acherusicum of yellow beak at present, mainly lure the collection to observe with artifical honeycomb, not only work load is big, and is long consuming time moreover, lures a collection inefficiency moreover, and it is more to invest in manpower and materials. The adult bee has strong flying capability and certain aggressiveness, and is difficult to capture for laboratory research.
Disclosure of Invention
In order to solve the problems in the background art, the invention provides a device and a method for remotely observing and breeding corophium flavum, which solve the problems of difficulty in observation, low trapping efficiency and the like and reduce the risk of people being stunted during a test using adult bees.
Before, research and observation of hunting solitary bees with aggressiveness such as corophium rhynchopsicum need to be carried out in the field, or the solitary bees are brought back to a laboratory after being caught and trapped by a net, so that a certain risk of being bitten is brought to a laboratory; the bees have stronger flight capability and low net catching efficiency, which brings difficulty to further research and is an important reason for less published amount of relevant research papers of the bees. The device of the invention collects the wild scattered hunting solitary bee resources in the insect cage room, brings research convenience for scientific researchers, and greatly improves the research output efficiency.
The technical scheme adopted by the invention is as follows:
yellow corophium acherusicum remote observation and reproduction device
The potter comprises an insect cage, wherein the insect cage is a square box body, the right side surface of the insect cage is provided with a zipper door with the height smaller than that of the insect cage, a collecting channel communicated with the interior of the insect cage is arranged in the middle of the left side surface of the insect cage and close to the lower position, and is used for collecting potter acherusicum, the middle part of the collecting channel is provided with a horn mouth with a small outside and a large inside, the diameter of the large mouth of the horn mouth is the same as that of the collecting channel, rope drawing holes are formed in the positions of the large mouth of the collecting channel and the outlet position, and a drawing rope is threaded through the rope drawing holes; an artificial nest made of PVC (polyvinyl chloride) tubes is arranged in the insect-raising cage and at the position close to the intersection of the upper side and the front side, and the interior of each PVC tube is filled with reed tubes; the inside and be close to collection passageway apex angle position department of insect feeding cage installs the camera.
The insect-raising cage and the collecting channel are made of 80-mesh light-transmitting nylon yarn materials.
A unilateral light source for irradiating the collecting channel is arranged above one side of the insect-raising cage close to the collecting channel.
Two culture dishes with diameters different by 1 time and height are placed on the bottom surface of the insect cage, water is injected into the small culture dish, and wet mud is filled in the large culture dish. The combination of water and wet mud can meet the external conditions required by adult bees to nest and does not pollute the environment in the cage.
Seal through tightening up the drawstring and collect passageway and external communicating export, the size that supplies yellow corophium acherusicum to pass through is designed to the inside diameter of horn mouth aperture of corophium acherusicum collection passageway, and yellow corophium acherusicum can only be followed the horn mouth macrostoma and is flown out the osculum, is difficult to follow the horn mouth macrostoma and flies out the macrostoma.
The side surface of the PVC pipe is provided with a zipper which is convenient for storing and taking the reed pipe.
Second, remote observation and propagation method of corophium acherusicum
The method comprises the following steps:
step 1: placing the insect cage in an artificial climate chamber or an artificial incubator, placing one surface of a collecting channel of the insect cage on the side close to a light source, and placing one surface of a zipper door of the insect cage on the side of a far light source;
step 2: a plurality of reed pipes with at most one node are placed in the PVC pipe, and the PVC pipe is tightly packed by the reed pipes, so that the reed pipes are not easy to fall off; putting 10% of honey water into a small culture dish, adding a proper amount of absorbent cotton, and slightly touching the absorbent cotton until the honey water overflows; confirming that the drawstring at the horn mouth of the collecting channel is completely tightened, closing the zipper mouth at the PVC pipe, opening the zipper door and putting the zipper door into the channel through CO after the camera normally works2Narcotizing corophium acherusicum wasp;
and step 3: the corophium rhynchophyllum performs mating, food taking and nesting activities in the daytime, the activity peak period is 11-13 pm, the flying activity gradually disappears after 7 pm, most rakes rest at the near light source side, and a few individuals hide in empty reed pipes for rest. Therefore, after 7 pm every day, cage environment maintenance and food addition of the insect cage can be performed, and the insect cage is not easy to attack if the action needs to be noticed; adding honey water into the absorbent cotton by using an injection needle tube every day, and replacing a culture dish and the absorbent cotton every other day;
and 4, step 4: after about one week, when the cameras record that adult bees mate in the insect rearing cage, the hunting is started to be thrown in for 2-3 years, the throwing amount of the hunting is calculated according to the fact that 2-3 hunting needs to be eaten by each mated female bee, and the hunting consumption is observed in the stage and is supplemented a little day by day; the input amount depends on the number of female bees which have mated, and one female bee can eat 2-3 preys per se in the pre-laying period.
And 5: one week after the first female bee mating, increasing the input amount of prey, wherein the input amount is calculated according to the requirement of 20 prey of 2-3 years old for each mated female bee; generally, 1 female bee can complete the construction of 1 nest tube in one day, 2 nest chambers are arranged in a single nest tube, 10 prey meters with 2 ages and the last to 3 ages are stored in each nest chamber, and the input amount of the prey is calculated. In the stage, the male bees which finish mating die gradually, the male bee corpses in the insect cage are collected in time, and the remained prey bodies are cleaned; adding water into the small culture dish by using an injection needle tube every two days, and keeping the soil in the large culture dish moist; during the period of laying eggs and nesting of female bees, a proper amount of honey water needs to be taken for supplementing nutrition, so the honey water cannot be withdrawn.
Step 6: after the nest tube is sealed and the sealing mud is dried, taking out the nest tube through a zipper opening of the PVC tube, and supplementing a new nest tube in time; the nest tube is a reed tube.
And 7: collecting corophium wasp adult bees for testing: opening a horn mouth of the collecting channel to draw the rope at night before or in the morning of collection, keeping the drawing rope at the outlet position of the collecting channel to be tightened, leading adult bees to enter a cavity between the horn mouth and the collecting channel from the horn mouth under the induction of a light source, and tightening the drawing rope at the horn mouth after the number of the adult bees entering the cavity is enough; a container with a sealed space formed in the cavity on the collecting channel cover, and introducing CO into the container from the outside2Bee quilt CO in the cavity2After anaesthesia, the drawstring at the outlet position of the collecting channel is opened and the adult bees are taken out.
The temperature of the artificial climate chamber or the artificial incubator is regulated to be 25-30 ℃; regulating and controlling the relative humidity RH to 30-50%; the illumination sunshine period is regulated and controlled to be L: d is 16: 8-12: 12.
The number of corophium acherusicum adult bees placed in the insect rearing cage in the step 2 is 20, wherein the ratio of males to females is 2: 1.
The prey is artificially propagated or field-captured lepidoptera larvae such as prodenia litura, cotton bollworm, pink bollworm, rice stem borer or cabbage caterpillar.
The invention has the beneficial effects that:
1) the device provided by the invention can be used for indoor propagation and observation of hunting solitary bees, and can be used for self-subculture propagation after providing simple necessary living conditions for the bees, so that the trouble of collecting along with use is avoided, the supply efficiency of a research object is improved, the scientific research output efficiency is improved, and the labor and the sting risk of field collection of scientific research personnel are reduced.
2) The device provided by the invention is provided with the camera, can continuously record the activity condition of the bees in the cage all day long, retains the influence data, is visual and reliable, can be used for further analysis and research, and avoids the careless omission caused by manual observation. If the field conditions are appropriate, the device can be moved to the outside for real-time observation under open conditions.
3) The zipper arranged on the side surface of the PVC pipe is convenient for timely taking out the nested nest pipe and adding a new reed pipe, the zipper door of the cage does not need to be opened, the whole artificial nest is taken out, and the escape of adults in the cage and the risk of being bitten by technicians are avoided; the horn mouth which can be controlled to be opened and closed by the drawstring is arranged, the cage door does not need to be opened, the adult bees required by the experiment can be easily obtained, and the risk of being bitten when the insects are taken is reduced.
4) According to the potter acherusicum potter provided by the invention, by utilizing the phototaxis characteristic of potacherusicum potench at night, the unilateral light source is arranged, so that bee colonies can be induced to gather at one side of the cage room, the daily cage room maintenance is facilitated, for example, foodstuff is added, reed pipes are replaced, and the risk of being bitten or bees escaping is avoided. Utilize yellow corophium acherusicum characteristic of phototaxis gathering night equally, set up the horn-shaped opening of easily advancing difficult play in the light source side, can make the bee gather in a narrower and small space, then the researcher of being convenient for acquires into the bee easily and carries out research next step.
Drawings
FIG. 1 is a schematic diagram of the apparatus of the present invention.
In the figure: the insect breeding cage (1), the zipper door (2), the channel (3), the PVC pipe (4), the remote camera (5), the small culture dish (6) and the big culture dish (7)
Detailed Description
The invention is further illustrated by the following figures and examples.
As shown in fig. 1, the insect cage comprises an insect cage 1, the insect cage 1 is a square box body made of nylon yarn materials, a zipper door 2 with the height smaller than that of the insect cage 1 is arranged on the right side of the insect cage 1, a collecting channel 3 communicated with the interior of the insect cage 1 and used for collecting corophium is arranged on the left side of the insect cage 1 and in the middle and at the lower position, a horn mouth with a small outer part and a large inner part is arranged at a position 15cm away from the collecting channel 3, the diameter of the large mouth of the horn mouth is the same as that of the collecting channel 3, rope drawing holes are arranged at the large mouth position and the outlet position of the collecting channel 3, and a drawing rope is threaded through the rope drawing holes; an artificial nest made of PVC pipes 4 is arranged in the insect-raising cage 1 and at a position close to the intersection of the upper side and the front side, and the interior of each PVC pipe 4 is filled with reed pipes; inside and being close to 3 apex angle positions departments of collecting channel of insect feeding cage 1 and installing camera 5 and be used for remote observation.
In specific implementation, the length, the width and the height of the square box body are both 50 cm; the height of the zipper door is 30 cm; the diameter of the collecting channel 3 is 10cm, and the length is 35 cm; the length of the bell mouth is 9cm, the diameter of the small mouth is 2cm, and the diameter of the large mouth is 8 cm; the length of the PVC pipe is 20cm, the inner diameter is 11cm, and the suspension height is 2cm away from the upper side; the length of the reed pipe is 18-22 cm, and the inner diameter is 1 cm; the length of a zipper opening of the PVC pipe 4 is 22cm, and the vertical distance between the zipper opening and the upper top surface is 7 cm; the diameter of the large culture dish 7 is 10cm, the diameter of the small culture dish 6 is 5cm, and the heights of the large culture dish and the small culture dish are all about 2 cm.
Example (b):
1. regulating and controlling the temperature, humidity and illumination conditions of an artificial climate chamber or an artificial incubator: controlling the temperature to be 25-30 ℃; the illumination conditions are L: d is 16: 8-12: 12; RH is 30-50%. The insect breeding cage 1 is placed in an artificial climate room, one surface of the horn mouth is placed on the side close to the light source, and one surface of the zipper door of the insect breeding cage 1 is positioned on the side far from the light source.
2. At most one reed pipe with one knot is put into the PVC pipe 4, the packing is tight, and the reed pipe is not easy to fall. Putting 10% of honey water into the small culture dish 6, adding a proper amount of absorbent cotton, and slightly touching the absorbent cotton until the honey water overflows. Confirming that the drawstring at the bell mouth is completely tightened, closing the zipper mouth at the 4 position of the PVC pipe, and putting the PVC pipe into the zipper through CO from the zipper door after the camera normally works2Narcotizing corophium acherusicum adult wasps. 20 adult bees can be placed in a single rearing cage, and the ratio of the female insects to the male insects is 2: 1.
3. The corophium rhynchophyllum performs mating, food taking and nesting activities in the daytime, the activity peak period is 11-13 pm, the flying activity gradually disappears after 7 pm, most rakes rest at the near light source side, and a few individuals hide in empty reed pipes for rest. Therefore, after 7 pm, simple cage environment maintenance, food adding and the like can be performed, and the attack is not easy to occur if the action needs to be careful. Adding honey water to the absorbent cotton by using an injection needle tube every day, and replacing a culture dish and the absorbent cotton every other day.
4. After about one week, after the camera records that the adults in the insect cage 1 have mating behaviors, 2-3-year-old lepidoptera larvae (hereinafter, referred to as 'preys') such as prodenia litura, cotton bollworm, pink bollworm, rice stem borer or cabbage caterpillar, which are artificially propagated or captured in the field, can be thrown in, the throwing amount is determined according to the number of female bees which have mated, and the amount of corophium wasps which have mated can be recorded by the camera. Generally, a female bee can eat 2-3 preys per se in the early stage of spawning. This phase requires observation of prey consumption with small daily supplements.
5. And (3) continuously supplying wet mud and water to the first female bees about one week after mating, and slightly increasing the input amount of prodenia litura. Generally, 1 female bee can complete the construction of 1 nest tube in one day, a single tube is used for 2 nest chambers, 10 prey meters with 2 ages and the last to 3 ages are stored in each nest chamber, and the input amount of the prey is calculated. At this stage, the male bees which finish mating will die gradually, and the male bee bodies need to be collected and cleaned to remove the residual prey bodies in the female bees. And (4) adding water into the water storage box by using an injection needle tube every two days, and keeping the soil wet. During the period of laying eggs and nesting of female bees, a proper amount of honey water needs to be taken for supplementing nutrition, so the honey water cannot be withdrawn.
6. After the nest tube is sealed and the sealing mud is dried, the nest tube is taken out through the zipper opening of the PVC tube 4, the next observation is carried out, and a new nest tube is supplemented in time.
7. If corophium wasps need to be collected for testing, the pull rope 2 at the horn mouth is opened in the evening or the morning of the day, the pull rope 1 of the collecting channel 3 is kept to be tightened, the adult wasps enter a cavity between the horn mouth and the collecting channel 3 from the horn mouth under the attraction of a light source, and the pull rope 2 at the horn mouth is tightened after the number of the adult wasps entering the cavity is enough; a container for forming a closed space in the cavity on the outer cover of the collecting channel 3, and introducing CO into the container from the outside2Bee quilt CO in the cavity2After anaesthesia, the drawstring 1 of the collection channel 3 is opened and the adult bees are taken out.
The indoor embodiment of the invention is not limited to indoor, and the device can be installed outdoors under the condition of ensuring safety and is used for observing behaviors such as nesting behavior and eclosion rhythm of potter.
Claims (4)
1. The utility model provides a corophium acherusicum remote observation and reproduction method, it adopts corophium acherusicum remote observation and reproduction device, corophium acherusicum remote observation and reproduction device includes insect cage (1), insect cage (1) is square box, open on insect cage (1) right flank has height to be less than zipper (2) of insect cage (1) height, insect cage (1) left flank leans on the lower position to be provided with in the middle with insect cage (1) inside communicating be used for collecting corophium acherusicum collection passageway (3), the middle part of collection passageway (3) is provided with the horn mouth that is little inside big, the big mouth diameter of horn mouth is the same with collection passageway (3) diameter, exit position department and the big mouth position department of horn mouth of collection passageway (3) all are provided with the rope drawing hole, wear the rope drawing through the rope hole; an artificial nest made of PVC pipes (4) is arranged in the insect-raising cage (1) and at a position close to the intersection of the upper side and the front side, and the interior of each PVC pipe (4) is filled with reed pipes; a Wifi remote camera (5) is arranged at the vertex angle position inside the insect breeding cage (1) and close to one side of the collecting channel (3); the insect breeding cage (1) and the collecting channel (3) are made of 80-mesh light-transmitting nylon yarn materials; a single-side light source for irradiating the collecting channel (3) is arranged above one side of the insect breeding cage (1) close to the collecting channel (3); two culture dishes with diameters different by 1 time and height equal are placed on the bottom surface of the insect cage (1), water is injected into the small culture dish (6), and wet mud is filled in the large culture dish (7); an outlet of the collecting channel (3) communicated with the outside is closed by tightening the drawstring, and the inner diameter of the small opening of the horn mouth of the collecting channel (3) is designed to be the size for the yellow potter achy to pass through in a single direction; the side surface of the PVC pipe (4) is provided with a zipper opening to facilitate the reed pipe to be stored and taken;
the method is characterized by comprising the following steps:
step 1: the insect breeding cage (1) is placed in an artificial climate room or an artificial incubator, one surface of a collecting channel (3) of the insect breeding cage (1) is placed on the side close to a light source, and one surface of a zipper door (2) of the insect breeding cage (1) is positioned on the side of a far light source;
step 2: a plurality of reed pipes with at most one node are placed in the PVC pipe (4), and the PVC pipe (4) is tightly packed by the reed pipes, so that the reed pipes are not easy to fall off; putting 10% honey water into a small culture dish (6), and adding absorbent cotton; confirm that the flare opening department of collecting passageway (3) the drawstring is taut completely, PVC pipe (4) department zip fastener mouth is closed, and after Wifi remote camera normally worked, open zip fastener door (2) and put into through CO2Narcotizing corophium acherusicum wasp;
and step 3: after 7 pm every day, cage room environment maintenance and food addition of the insect rearing cage (1) are carried out; adding honey water into the absorbent cotton by using an injection needle tube every day, and replacing a culture dish and the absorbent cotton every other day;
and 4, step 4: when the Wifi remote camera (5) records that adult bees mate in the insect rearing cage (1), 2-3 instars of prey are put in, and the input amount of the prey is calculated according to the requirement that 2-3 preys are eaten by each mated female bee;
and 5: one week after the first female bee mating, increasing the input amount of prey, wherein the input amount is calculated according to the requirement of 20 prey of 2-3 years old for each mated female bee; in the stage, the male bees which finish mating die gradually, the male bee corpses in the insect rearing cage (1) are collected in time, and the remained prey bodies are cleaned; adding water into the small culture dish (6) by using an injection needle tube every two days, and keeping soil in the large culture dish (7) moist;
step 6: after the nest tube is sealed and the sealing mud is dried, taking out the nest tube through a zipper opening of the PVC tube (4), and supplementing a new nest tube in time; the nest pipe is a reed pipe;
and 7: collecting corophium wasp for test: opening the drawing rope at the bell mouth of the collecting channel (3) at night before or in the morning before collection, keeping the drawing rope at the outlet position of the collecting channel (3) tightened, leading the adult bees to enter a cavity between the bell mouth and the collecting channel (3) from the bell mouth under the attraction of a light source, and tightening the bell mouth after the number of the adult bees entering the cavity is enoughDrawing the rope; a container which forms a closed space in the cavity on the outer cover of the collecting channel (3), and CO is introduced into the container from the outside2Bee quilt CO in the cavity2After anaesthesia, the drawstring at the outlet position of the collecting channel (3) is opened and the adult bees are taken out.
2. The corophium acherusicum remote observation and propagation method according to claim 1, wherein the temperature of the artificial climate chamber or artificial incubator is controlled to be 25-30 ℃; regulating and controlling the relative humidity to 30-50%; the illumination period is regulated to be L: d =16: 8-12: 12.
3. The corophium acherusicum remote observation and propagation method according to claim 1, characterized in that the corophium acherusicum adult bees put in the insect rearing cages (1) in the step 2 are 20 heads, wherein the ratio of the female bees and the male bees in the corophium acherusicum adult bees is 2: 1.
4. The corophium acherusicum remote observation and propagation method according to claim 1, characterized in that the prey is prodenia litura, cotton bollworm, pink bollworm, rice stem borer or cabbage caterpillar which is artificially propagated or captured in the field.
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