CN113615587B - Experimental device and method for researching predation behavior in symbiotic mode - Google Patents
Experimental device and method for researching predation behavior in symbiotic mode Download PDFInfo
- Publication number
- CN113615587B CN113615587B CN202110977335.9A CN202110977335A CN113615587B CN 113615587 B CN113615587 B CN 113615587B CN 202110977335 A CN202110977335 A CN 202110977335A CN 113615587 B CN113615587 B CN 113615587B
- Authority
- CN
- China
- Prior art keywords
- experimental
- predators
- box
- experiment
- period
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000000034 method Methods 0.000 title abstract description 7
- 238000002474 experimental method Methods 0.000 claims abstract description 112
- 244000062645 predators Species 0.000 claims abstract description 100
- 238000012360 testing method Methods 0.000 claims abstract description 8
- 230000000694 effects Effects 0.000 claims abstract description 5
- 238000009395 breeding Methods 0.000 claims abstract description 4
- 230000001488 breeding effect Effects 0.000 claims abstract description 4
- 241000196324 Embryophyta Species 0.000 claims description 27
- 230000006399 behavior Effects 0.000 claims description 15
- 235000003642 hunger Nutrition 0.000 claims description 12
- 238000012258 culturing Methods 0.000 claims description 9
- 230000037351 starvation Effects 0.000 claims description 9
- 241000592344 Spermatophyta Species 0.000 claims description 8
- 241000238631 Hexapoda Species 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 230000004071 biological effect Effects 0.000 claims description 3
- 230000003203 everyday effect Effects 0.000 claims description 3
- 230000000384 rearing effect Effects 0.000 claims description 3
- 238000010998 test method Methods 0.000 claims 1
- 238000005259 measurement Methods 0.000 abstract description 3
- 238000005286 illumination Methods 0.000 description 9
- 241000209094 Oryza Species 0.000 description 7
- 235000007164 Oryza sativa Nutrition 0.000 description 7
- 235000009566 rice Nutrition 0.000 description 7
- 241000607479 Yersinia pestis Species 0.000 description 5
- 238000012986 modification Methods 0.000 description 5
- 230000004048 modification Effects 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- NIXOWILDQLNWCW-UHFFFAOYSA-N acrylic acid group Chemical group C(C=C)(=O)O NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 4
- 230000006870 function Effects 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 230000008014 freezing Effects 0.000 description 2
- 238000007710 freezing Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 230000029264 phototaxis Effects 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 229910001220 stainless steel Inorganic materials 0.000 description 2
- 239000010935 stainless steel Substances 0.000 description 2
- 241000238557 Decapoda Species 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 230000022542 predatory behavior Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 230000031068 symbiosis, encompassing mutualism through parasitism Effects 0.000 description 1
- 239000012780 transparent material Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K1/00—Housing animals; Equipment therefor
- A01K1/02—Pigsties; Dog-kennels; Rabbit-hutches or the like
- A01K1/03—Housing for domestic or laboratory animals
- A01K1/031—Cages for laboratory animals; Cages for measuring metabolism of animals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K1/00—Housing animals; Equipment therefor
- A01K1/02—Pigsties; Dog-kennels; Rabbit-hutches or the like
- A01K1/035—Devices for use in keeping domestic animals, e.g. fittings in housings or dog beds
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K31/00—Housing birds
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Animal Behavior & Ethology (AREA)
- Health & Medical Sciences (AREA)
- Clinical Laboratory Science (AREA)
- General Health & Medical Sciences (AREA)
- Birds (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses an experimental device and a method for researching predation behavior in a symbiotic mode, and relates to the technical field of biological control. By arranging the planting and breeding box, the phenomena of lodging, even breakage and the like of the plants caused by the activities of predators are avoided, and the experimental accuracy is improved; by arranging the lighting main board, all living predators are concentrated to one corner above the second experiment box, so that data measurement is facilitated; through the arrangement of the inlet and the outlet, predators can be quickly put in or taken out, and experimental data errors caused by taking out time difference are reduced; can be recycled for a plurality of times, and the test cost is reduced.
Description
Technical Field
The invention relates to the technical field of biological control, in particular to an experimental device and method for researching predation behaviors in a symbiotic mode.
Background
Animals will search for prey in their environment for their own lives and develop a certain predation, resulting in a reduced population of prey. The study of predation behavior in animals is crucial for the study of biological control.
The research on predation behavior of symbiotic cultures in rice fields in symbiotic mode can provide theoretical basis for biological control. In the paddy field, the field pests are used as predators, the symbiotic culture is used as predators, the predators predate the predators, the number of the field pests can be effectively reduced through predation behaviors and stress, and the influence of the field pests on the paddy field is reduced. The experimental device designed based on the method can simulate the paddy field environment and symbiotic mode and study the predation behavior of symbiotic cultures (predators).
The prior art experimental device for testing the predation function reaction of paddy fields, as shown in fig. 1, comprises a first experimental box 1 for containing predators and paddy rice, and a second experimental box 2 for containing predators; the first laboratory box 1 is a dangerous area. The existing experimental device for testing the rice field predation function reaction has the following defects: 1. the planting of rice in the experimental apparatus is influenced by predators, and the phenomenon of collapse easily occurs, so that the capability of the predators for searching for prey is enhanced, the predation effect is further enhanced, and experimental errors are caused: 2. after the experiment is finished, the number of living bodies of the predators remained in the experimental device is difficult to quantify, and experimental errors are caused: 3. the experimental device cannot be reused, and the experimental cost is high; 4. the experimental device cannot shoot the physiological activities generated when predators prey on prey objects (predators and field pests). A new experimental set-up or experimental means (method) is needed to avoid one or more of the above-mentioned disadvantages.
Disclosure of Invention
Therefore, the invention provides an experimental device and a method for researching predation behaviors in a symbiotic mode, so as to solve the technical problems.
In order to achieve the above object, the present invention provides the following technical solutions:
in a first aspect of the invention, an experimental set-up for studying predation behaviour in symbiotic mode, comprises:
a first laboratory box for housing predators;
the second experiment box is used for accommodating predators, is arranged at the top of the first experiment box and is communicated with the first experiment box, and a switchable inlet and a switchable outlet are arranged at the side part of the second experiment box;
the planting and breeding box is used for transplanting planting plants and is arranged in the first experiment box;
the illumination mainboard is used for attracting and gathering predators, the illumination mainboard is arranged at one corner of the top of the second experiment box, and light emitted by the illumination mainboard faces into the experiment box.
Further, the experimental device further comprises a camera system, a transparent plate is arranged at the center of the top of the second experimental box, the diameter of the transparent plate is 5cm, a lens of the camera system is arranged on the upper side of the transparent plate, and the view angle of the lens faces into the experimental box.
Further, the first experiment box, the second experiment box and the planting and raising box are of cube structures, the length, width and height of the first experiment box are 30 multiplied by 25cm, the length, width and height of the second experiment box are 30 multiplied by 45cm, the side walls of the first experiment box and the planting and raising box are opaque acrylic plates, and the side wall of the second experiment box is a 120-mesh spun silk net.
Further, the second experiment box comprises main body frame strips of stainless steel and the gauze, the main body frame strips are provided with a plurality of main body frame strips, the main body frame strips form a cube framework, the cube framework is fixed at the top opening of the first experiment box, and the gauze is coated outside the cube framework.
Further, the inlet and outlet are arranged on the gauze at the side part, a zipper is arranged at the inlet and outlet, and the length of the zipper is 30cm.
In a second aspect of the invention, an experimental method for studying predation behaviour in symbiotic mode, using an experimental device according to any of claims 1-5, performs the following steps in one experimental cycle:
temporarily culturing predators in a first experiment box for a plurality of times, and culturing the adaptability of the predators in the first experiment box;
transplanting the plants meeting the experimental standard into a planting and breeding box, and enabling the plants to grow in an experimental device until the experiment is finished;
placing the predators in the same environment as the second experiment box, and culturing the adaptability of the predators in the second experiment box;
starving predators adapted to the first laboratory box environment;
the predators adapting to the environment of the second experiment box are placed into the second experiment box according to the corresponding density, and meanwhile, predators after starvation treatment are placed into the first experiment box according to the corresponding density;
the number of predations was recorded, the number of predators in different areas of the laboratory box was recorded, and the number of living bodies of the predators was recorded at the end of the experiment.
Further, the experimental period is 10 days, including a first experimental period of 1-7 days, a second experimental period of 2-10 days, a third experimental period of 5-8 days, a fourth experimental period of 8 days, a fifth experimental period of 9 days, and a sixth experimental period of 10 days, wherein:
in the first experimental time period, each predator is correspondingly placed into one first experimental box for moving for 120 minutes every day, so that each predator can move in the corresponding first experimental box to adapt to the environment of the corresponding first experimental box, and the predator bodies are used for feeding to generate predation memory;
in the second experimental time period, correspondingly placing the cultivated plants meeting the experimental standard into the planting and raising boxes in the first experimental box and injecting water, so that the planting plants can grow for one week or more in the corresponding planting and raising boxes to adapt to the environment in the corresponding planting and raising boxes, and the phenomena of withering and the like of the planting plants during the experimental period are prevented, and experimental errors are caused;
in the third experimental period, placing the insect rearing cages of the predators into the same conditions as the experimental environment for three days and more, so that each predator can grow in the corresponding experimental environment to adapt to the environment in the corresponding second experimental box;
in the fourth experimental period, taking out each predator cultured in the first experimental period correspondingly for unified temporary culture, and carrying out starvation treatment for 24 hours;
in a fifth experimental time period, the predators cultured in the third experimental time period are uniformly placed in a second experimental box according to the corresponding density, and predators subjected to starvation treatment in the fourth experimental time period are uniformly placed in a first experimental box according to the corresponding density under the same time;
in the sixth experimental period, after the predator and the predator were co-located in the experimental box for 24 hours, the predator was taken out at the first time, the lighting facility was turned on, the number of living bodies of the predator was observed after one hour, and experimental data was recorded.
Further, seeds of the seed plants and experimental predators were first cultivated in an illumination incubator before the first experimental period to reach a plant height of 35-45 cm to meet experimental standards.
Further, in the fifth and sixth experimental time periods, the camera system records the biological activity condition in the experimental box.
Further, the different areas of the experiment box comprise two dangerous areas of the water surface of the first experiment box and the height range of 0-10 cm of the seed plants in the second experiment box, and three driving-away areas of the height range of 10-20 cm, the height range of 20-35 cm and the net box of the seed plants in the second experiment box.
The invention has the following advantages:
according to the experimental device provided by the invention, the planting and raising box is arranged in the first experimental box, so that the phenomena of lodging, even breakage and the like of the plants due to the activities of predators can be avoided when the plants are transplanted or planted in the first experimental box, and the experimental accuracy is improved; by arranging the lighting main board, all living predators are concentrated to one corner above the second experiment box by utilizing the phototaxis of insects, so that the measurement of experimental data is convenient, the experimental efficiency is improved, and the experimental accuracy is further improved; through setting the switchable access, predators can be quickly placed before an experiment, and the predators can be quickly taken out after the experiment is finished, so that the error of experimental data caused by taking out time difference is reduced; after the experiment is finished, the experimental device can be recycled for multiple times, so that the experiment cost is reduced; in addition, by arranging the camera system, image data can be provided for experimental results, so that the persuasion of experiments is improved. The experimental method provided by the invention adopts the experimental device, has the advantage of high accuracy brought by the experimental device, and has reasonable steps, high experimental efficiency, simplicity and easiness in operation.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below. It will be apparent to those skilled in the art from this disclosure that the drawings described below are merely exemplary and that other embodiments may be derived from the drawings provided without undue effort.
The structures, proportions, sizes, etc. shown in the present specification are shown only for the purposes of illustration and description, and are not intended to limit the scope of the invention, which is defined by the claims, so that any structural modifications, changes in proportions, or adjustments of sizes, which do not affect the efficacy or the achievement of the present invention, should fall within the scope of the invention.
Fig. 1 is a schematic structural diagram of an experimental device for testing a predation function reaction of a paddy field according to the background art of the invention;
FIG. 2 is a schematic structural diagram of an experimental device for studying predation behavior in symbiotic mode according to an embodiment of the present invention;
fig. 3 is a flow chart of an experimental method for studying predation behavior in symbiotic mode according to an embodiment of the present invention.
In the figure: 1-first experiment box, 2-second experiment box, 3-planting and raising box, 4-main body frame strip, 5-illumination mainboard, 6-transparent plate, 7-zip fastener, 8-camera system.
Detailed Description
Other advantages and advantages of the present invention will become apparent to those skilled in the art from the following detailed description, which, by way of illustration, is to be read in connection with certain specific embodiments, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
The terms such as "upper", "lower", "left", "right", "middle" and the like are also used in the present specification for convenience of description, but are not intended to limit the scope of the present invention, and the changes or modifications of the relative relationship thereof are considered to be within the scope of the present invention without substantial modification of the technical content.
As shown in fig. 2, one embodiment of the present invention provides an experimental apparatus for studying predation behavior in symbiotic mode, which includes a first experimental tank 1, a second experimental tank 2, a planting tank 3 (transplanting or planting rice), an illumination apparatus (including an illumination main board 5), and a camera system 8 (including a lens).
The first laboratory box 1 is intended to accommodate predators. In an alternative scheme of the embodiment, the first experiment box 1 is a cube box body with a wide opening at the top end, and the length, width and height of the first experiment box 1 are 30×30×25cm, and the first experiment box 1 is made of transparent acrylic plates.
The second laboratory box 2 is generally cube-shaped for receiving predators. In an alternative scheme of the implementation, the overall length and width of the second experiment box 2 are 30×30×45cm, the second experiment box 2 comprises a cube framework and 120 mesh gauze wrapped outside the cube framework, the cube framework mainly comprises a plurality of main frame strips 4 made of stainless steel, the cube framework is fixed at the top opening of the first experiment box 1, the main frame strips 4 are generally positioned at four corners of the first experiment box 1, the gauze wraps the front, the rear, the left and the right and the upper sides of the cube framework, the lower side edge of the gauze is bonded with the wall plate of the first experiment box 1, and the gauze is not arranged at the lower side of the cube framework, so that the communication between the first experiment box 1 and the second experiment box 2 is ensured, and a predator can capture a predator. The lateral part of the second experiment box 2 is provided with a switchable access opening, in this embodiment, the access opening is arranged on the gauze of the lateral part, the access opening is provided with a zipper 7, and the length of the zipper 7 is 30cm.
The planting and cultivating box 3 is used for transplanting plants, and can also be used for directly planting the plants, and is a cube container with an opening at the top, the side wall is an opaque acrylic plate, and the planting and cultivating box 3 is arranged in the first experiment box 1.
The lighting main board 5 of the lighting device is arranged at one corner of the top of the second experiment box 2, and the light emitted by the lighting main board 5 faces the inside of the experiment box and is used for attracting and gathering predators.
The top center of the second experiment box 2 is provided with a transparent plate 6, which is generally made of colorless transparent materials, acrylic materials or glass materials can be adopted, the diameter of the transparent plate 6 is 5cm, the lens of the camera system 8 is arranged on the upper side of the transparent plate 6, and the field angle of the lens faces into the experiment box.
By arranging the planting and raising box 3 in the first experiment box 1, when the plants are transplanted or planted in the first experiment box, the phenomena of lodging, even breakage and the like of the plants due to the activities of predators are avoided, and the experiment accuracy is improved; by arranging the illumination main board 5, all living bodies are concentrated to one corner above the second experiment box 2 by utilizing the phototaxis of insects, so that the measurement of experimental data is convenient, the experimental efficiency is improved, and the experimental accuracy is further improved; through setting the switchable access, predators can be quickly placed before an experiment, and the predators can be quickly taken out after the experiment is finished, so that the error of experimental data caused by taking out time difference is reduced; after the experiment is finished, the experimental device can be recycled for multiple times, so that the experiment cost is reduced; in addition, by arranging the camera system 8, image data can be provided for experimental results, so that the convincing power of experiments is improved.
As shown in fig. 3, another embodiment of the present invention provides an experimental method for researching predatory behavior in symbiotic mode, using the experimental apparatus described above, the following steps are performed in an experimental period of 10 days (taking rice-crab symbiosis as an example, rice is used as a seed plant, crabs are used as predators, and rice field pests are used as predators):
step S1, temporarily culturing predators in a first experiment box for a plurality of times, and culturing the adaptability of the predators in the first experiment box. Step S1 is carried out in a first experiment time period (1 st to 7 th days), each predator is correspondingly placed into one first experiment box for moving for 120 minutes every day, so that each predator can move in the corresponding first experiment box to adapt to the environment of the corresponding first experiment box, and the predator insects are used for feeding to generate predation memory
And S2, transplanting the plants meeting the experimental standards into a planting box, and enabling the plants to grow in an experimental device until the experiment is finished. Step S2 is carried out in a second experiment time period (day 2-10), the cultivated plants meeting the experiment standard are correspondingly placed in the planting and raising boxes in the first experiment box and injected with water, so that the planting plants can grow for one week or more in the corresponding planting and raising boxes to adapt to the environment in the corresponding planting and raising boxes, and the phenomena of withering and the like of the planting plants in the experiment period are prevented, and experimental errors are caused.
And S3, placing the predators into the same environment as the second experiment box, and culturing the adaptability of the predators in the second experiment box. And step S3, in a third experimental period (5 th to 8 th days), placing the insect rearing cages of the predators into the same conditions as the experimental environment for three days or more, so that each predator can grow in the corresponding experimental environment to adapt to the environment in the corresponding second experimental box.
And S4, starving predators adapting to the environment of the first experiment box. Step S4 is carried out in a fourth experiment time period (day 8), and each predator cultured in the first experiment time period is taken out correspondingly and is subjected to unified temporary culture, and starvation treatment is carried out for 24 hours or more.
And S5, placing predators adapting to the environment of the second experiment box into the second experiment box according to the corresponding density, and simultaneously placing predators subjected to starvation treatment into the first experiment box according to the corresponding density. Step S5 is carried out in a fifth time period (day 9), predators cultured in a third experiment time period are uniformly placed in a second experiment box according to the corresponding density, and predators subjected to starvation treatment in a fourth experiment time period are uniformly placed in a first experiment box according to the corresponding density under the same time.
S6, recording predation times, recording the number of predators in different areas of the experiment box, and recording the number of living bodies of the predators when the experiment is finished; the different areas of the experiment box comprise two dangerous areas of the water surface of the first experiment box and the height range of 0-10 cm of the plant planted in the second experiment box, and three driving-away areas of the height range of 10-20 cm, the height range of 20-35 cm and the net box of the plant planted in the second experiment box. Step S6, in a sixth time period (day 10), after the predators and the predators are co-located in the experiment box for 24 hours, taking out the predators at the first time, turning on the lighting facilities, observing the number of living bodies of the predators after one hour, and recording experimental data; sampling stomach and intestinal tract of predator taken out from the experimental device by using surgical scissors, stripping needle and forceps, rapidly freezing with liquid nitrogen, grinding in liquid nitrogen, extracting DNA, and freezing at-80deg.C; designing gene primers of predators by referring to NCBI gene library, amplifying the sampled DNA by using PCR amplification technology, carrying out electrophoresis treatment on the amplified product, observing the strip, sequencing after purifying the electrophoresis product, and comparing sequencing results to obtain a conclusion.
Before a first experiment time period, seeds of a seed plant and an experimental predator are cultivated in an illumination incubator, so that the height of the plant reaches 35-45 cm to meet experimental standards; in the fifth and sixth experimental time periods, the camera system records the biological activity condition in the experimental box.
The experimental method provided by the embodiment adopts the experimental device provided by the previous embodiment, has the advantage of high accuracy brought by the experimental device provided by the previous embodiment, and has reasonable steps, high experimental efficiency, simplicity and easiness in operation.
While the invention has been described in detail in the foregoing general description and specific examples, it will be apparent to those skilled in the art that modifications and improvements can be made thereto. Accordingly, such modifications or improvements may be made without departing from the spirit of the invention and are intended to be within the scope of the invention as claimed.
Claims (4)
1. An experimental method for researching predation behavior in symbiotic mode is characterized in that the following steps are executed in one experimental period by using an experimental device:
temporarily culturing predators in a first experiment box for a plurality of times, and culturing the adaptability of the predators in the first experiment box;
transplanting the plants meeting the experimental standard into a planting and breeding box, and enabling the plants to grow in an experimental device until the experiment is finished;
placing the predators in the same environment as the second experiment box, and culturing the adaptability of the predators in the second experiment box;
starving predators adapted to the first laboratory box environment;
the predators adapting to the environment of the second experiment box are placed into the second experiment box according to the corresponding density, and meanwhile, predators after starvation treatment are placed into the first experiment box according to the corresponding density;
recording predation times, recording the number of predators in different areas of an experiment box, and recording the number of living bodies of the predators when the experiment is finished;
the experimental period is 10 days, including a first experimental period of 1-7 days, a second experimental period of 2-10 days, a third experimental period of 5-8 days, a fourth experimental period of 8 days, a fifth experimental period of 9 days, and a sixth experimental period of 10 days, wherein:
in the first experimental time period, each predator is correspondingly placed into one first experimental box for moving for 120 minutes every day, so that each predator can move in the corresponding first experimental box to adapt to the environment of the corresponding first experimental box, and the predator bodies are used for feeding to generate predation memory;
in the second experimental time period, correspondingly placing the cultivated plants meeting the experimental standard into the planting and raising boxes in the first experimental box and injecting water, so that the planting plants can grow for one week or more in the corresponding planting and raising boxes to adapt to the environment in the corresponding planting and raising boxes, and the wilting phenomenon of the planting plants during the experimental period is prevented, and experimental errors are caused;
in the third experimental period, placing the insect rearing cages of the predators into the same conditions as the experimental environment for three days and more, so that each predator can grow in the corresponding experimental environment to adapt to the environment in the corresponding second experimental box;
in the fourth experimental period, taking out each predator cultured in the first experimental period correspondingly for unified temporary culture, and carrying out starvation treatment for 24 hours;
in a fifth experimental time period, the predators cultured in the third experimental time period are uniformly placed in a second experimental box according to the corresponding density, and predators subjected to starvation treatment in the fourth experimental time period are uniformly placed in a first experimental box according to the corresponding density under the same time;
in the sixth experimental period, after the predator and the predator were co-located in the experimental box for 24 hours, the predator was taken out at the first time, the lighting facility was turned on, the number of living bodies of the predator was observed after one hour, and experimental data was recorded.
2. An experimental method for studying predatory behaviour in symbiotic mode as claimed in claim 1 where the seeds of the seed plant and the experimental predators are cultivated in an illuminated incubator before the first experimental period to reach a plant height of 35-45 cm to meet the experimental criteria.
3. An experimental method for studying predatory behaviour in symbiotic mode as in claim 1 where the camera system records the biological activity in the experimental box during the fifth and sixth experimental time periods.
4. A method of testing predatory activity in symbiotic mode as claimed in any of claims 1 to 3 wherein the different areas of the test chamber comprise two dangerous areas of the water surface of the first test chamber and the 0-10 cm height range of the seed plant in the second test chamber, and three drive-off areas of the seed plant in the second test chamber, 10-20 cm height range, 20-35 cm height range and the net cage.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110977335.9A CN113615587B (en) | 2021-08-24 | 2021-08-24 | Experimental device and method for researching predation behavior in symbiotic mode |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110977335.9A CN113615587B (en) | 2021-08-24 | 2021-08-24 | Experimental device and method for researching predation behavior in symbiotic mode |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113615587A CN113615587A (en) | 2021-11-09 |
| CN113615587B true CN113615587B (en) | 2023-10-24 |
Family
ID=78387593
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110977335.9A Active CN113615587B (en) | 2021-08-24 | 2021-08-24 | Experimental device and method for researching predation behavior in symbiotic mode |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113615587B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115152665B (en) * | 2022-07-26 | 2023-10-20 | 郝雅 | Aquatic ecosystem predation pheromone ecological effect research device |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201733734U (en) * | 2010-07-09 | 2011-02-09 | 叶军 | Device for raising American frogs in ecological, environment-friendly and efficient way |
| CN103875608A (en) * | 2014-03-24 | 2014-06-25 | 华中农业大学 | Safety evaluation method of transgene insect-resistant rice on predative natural enemy cyrtorhinus lividipennis |
| CN206565116U (en) * | 2017-03-03 | 2017-10-20 | 中国农业科学院蜜蜂研究所 | A kind of easy device of indoor measurement honeybee acquisition capacity |
| CN109566428A (en) * | 2018-11-19 | 2019-04-05 | 中国科学院深圳先进技术研究院 | A kind of experimental provision and experimental method of the behavior of animal instinct fear |
| CN110074063A (en) * | 2019-04-30 | 2019-08-02 | 浙江大学 | Yellow beak Guo Luo remote reviewing and equipment for breeding and method |
| CN212087177U (en) * | 2020-04-30 | 2020-12-08 | 昭通学院 | Bionic cultivation experimental device for plant diseases and insect pests |
| CN112586446A (en) * | 2020-12-22 | 2021-04-02 | 湖南人文科技学院 | Energy distribution determination device and method for frog predation and avoidance behaviors under pressure of natural enemies |
| CN215736476U (en) * | 2021-08-24 | 2022-02-08 | 沈阳农业大学 | Experimental device for research predation action under symbiotic mode |
-
2021
- 2021-08-24 CN CN202110977335.9A patent/CN113615587B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201733734U (en) * | 2010-07-09 | 2011-02-09 | 叶军 | Device for raising American frogs in ecological, environment-friendly and efficient way |
| CN103875608A (en) * | 2014-03-24 | 2014-06-25 | 华中农业大学 | Safety evaluation method of transgene insect-resistant rice on predative natural enemy cyrtorhinus lividipennis |
| CN206565116U (en) * | 2017-03-03 | 2017-10-20 | 中国农业科学院蜜蜂研究所 | A kind of easy device of indoor measurement honeybee acquisition capacity |
| CN109566428A (en) * | 2018-11-19 | 2019-04-05 | 中国科学院深圳先进技术研究院 | A kind of experimental provision and experimental method of the behavior of animal instinct fear |
| CN110074063A (en) * | 2019-04-30 | 2019-08-02 | 浙江大学 | Yellow beak Guo Luo remote reviewing and equipment for breeding and method |
| CN212087177U (en) * | 2020-04-30 | 2020-12-08 | 昭通学院 | Bionic cultivation experimental device for plant diseases and insect pests |
| CN112586446A (en) * | 2020-12-22 | 2021-04-02 | 湖南人文科技学院 | Energy distribution determination device and method for frog predation and avoidance behaviors under pressure of natural enemies |
| CN215736476U (en) * | 2021-08-24 | 2022-02-08 | 沈阳农业大学 | Experimental device for research predation action under symbiotic mode |
Also Published As
| Publication number | Publication date |
|---|---|
| CN113615587A (en) | 2021-11-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106520541B (en) | A kind of kit of zebrafish embryo acute toxicity testing | |
| CN104521806B (en) | Culturing method of bee colony with 45-day pollination ability, and pollination bee colony culturing device | |
| CN113615587B (en) | Experimental device and method for researching predation behavior in symbiotic mode | |
| CN104396881A (en) | Propylaea japonica artificial breeding, breed conservation and propagation production method | |
| CN103141443A (en) | Method for regulating and controlling diapause of agryponflexorius | |
| CN215736476U (en) | Experimental device for research predation action under symbiotic mode | |
| CN107462699A (en) | A kind of detection method of aflatoxin B1 toxicity | |
| Beck et al. | Bean beetles, Callosobruchus maculatus, a model system for inquiry-based undergraduate laboratories | |
| US8776721B1 (en) | System and method for harvesting eggs from arthropods | |
| CN105532583B (en) | A kind of living body propagation method of entomopathogenic nematode | |
| CN109644947B (en) | Breeding method of lygus furcatus | |
| Green et al. | Interacting effects of environmental enrichment across multiple generations on early life phenotypes in zebrafish | |
| CN103931563B (en) | Transgenic pest-resistant rice is to predator Hylyphantes graminicolum method for evaluating safety | |
| CN110915764A (en) | Method for controlling Chinese bee queen isolation mating through gauze greenhouse | |
| CN108244061A (en) | A kind of Chinese blister beetle imago breeding device and Chinese blister beetle mating system | |
| Thompson et al. | Establishing an inexpensive, space efficient colony of Bemisia tabaci MEAM1 utilizing modelling and feedback control principles | |
| KR101010759B1 (en) | Development of cage for the cultivation of fruit flies | |
| Kam et al. | Maternal brood care of an arboreal breeder, Chirixalus eiffingeri (Anura: Rhacophoridae) from Taiwan | |
| CN113796357A (en) | Spider feeding system, spider feeding box and spider self-separation feeding method | |
| CN208286185U (en) | A kind of melittin administering transgenic breeding apparatus | |
| CN210671745U (en) | Free selection system for earthworm ecological factors | |
| CN108719210B (en) | Test method for detecting influence of pesticide on bee embryo development | |
| CN214229496U (en) | Large-scale clanis bilineata tsingtauica breeding device | |
| CN208657714U (en) | The quick living body separation of a kind of Large Arthropodss and can release device again | |
| CN218164037U (en) | Mosquito incubator |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |