CN109566428A - A kind of experimental provision and experimental method of the behavior of animal instinct fear - Google Patents
A kind of experimental provision and experimental method of the behavior of animal instinct fear Download PDFInfo
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- CN109566428A CN109566428A CN201811374996.7A CN201811374996A CN109566428A CN 109566428 A CN109566428 A CN 109566428A CN 201811374996 A CN201811374996 A CN 201811374996A CN 109566428 A CN109566428 A CN 109566428A
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- 238000002474 experimental method Methods 0.000 title claims abstract description 44
- 241001465754 Metazoa Species 0.000 title claims abstract description 41
- 244000062645 predators Species 0.000 claims abstract description 43
- 238000005192 partition Methods 0.000 claims abstract description 24
- 230000000694 effects Effects 0.000 claims description 22
- 239000000463 material Substances 0.000 claims description 21
- 239000012780 transparent material Substances 0.000 claims description 6
- 229910001220 stainless steel Inorganic materials 0.000 claims description 3
- 239000010935 stainless steel Substances 0.000 claims description 3
- 238000012360 testing method Methods 0.000 abstract description 15
- 244000062804 prey Species 0.000 abstract description 8
- 238000004891 communication Methods 0.000 abstract description 2
- 230000006399 behavior Effects 0.000 description 49
- 238000000034 method Methods 0.000 description 4
- 230000003466 anti-cipated effect Effects 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 241001269238 Data Species 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 101100067759 Mus musculus Gast gene Proteins 0.000 description 1
- 101100460719 Mus musculus Noto gene Proteins 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 230000003542 behavioural effect Effects 0.000 description 1
- 230000002490 cerebral effect Effects 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 210000000078 claw Anatomy 0.000 description 1
- 230000001143 conditioned effect Effects 0.000 description 1
- 230000008451 emotion Effects 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 210000005036 nerve Anatomy 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000013139 quantization Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000012549 training Methods 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K1/00—Housing animals; Equipment therefor
- A01K1/02—Pigsties; Dog-kennels; Rabbit-hutches or the like
- A01K1/03—Housing for domestic or laboratory animals
- A01K1/031—Cages for laboratory animals; Cages for measuring metabolism of animals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
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- Animal Behavior & Ethology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Health & Medical Sciences (AREA)
- Clinical Laboratory Science (AREA)
- General Health & Medical Sciences (AREA)
- Housing For Livestock And Birds (AREA)
Abstract
The present invention discloses the experimental provision and experimental method of a kind of animal instinct fear behavior, is related to technical field of bioengineering.Wherein experimental provision includes the camera system of top and the first experimental box for accommodating predator, is provided with multiple partitions in the experimental box of center, the space between the first experimental box and center experimental box is divided into multiple active box by multiple partitions；Prey can be accommodated in each second experimental box and is connected to by interface channel with corresponding active box；Each active box passes through an interface channel and is connected with one of them second experimental box, and opening in communication is all arranged in the junction of interface channel and active box and the second experimental box.The experimental method of animal instinct fear behavior disclosed by the invention uses above-mentioned experimental provision.Experimental provision disclosed by the invention can simultaneously test more preys, and use same predator, and experimental efficiency is high；Experimental method step is reasonable, and experimental efficiency is high, and experimental result is more accurate.
Description
Technical field
The present invention relates to technical field of bioengineering more particularly to a kind of experimental provisions and reality of the behavior of animal instinct fear
Proved recipe method.
Background technique
Frightened behavior has vital work as one of six kinds of basic emotion behaviors, for the existence of animal
With.Fear includes that conditioned fear and instinct are frightened, and wherein instinct fear, which refers to, does not need training study, for example, animal meeting
Environment, natural enemy and other risk factors locating for it are quickly judged and generate certain frightened behavior, so that
Animal can escape from danger obtain the possibility more survived.By the frightened cerebral nerve loop for forming behind of parsing, to above
The pathological study of illness, clinical research have very important significance.In the lab, since rat in the natural environment is small
The predator of mouse, mouse, which sees rat, can generate instinct fear, therefore often contrived experiment device based on this, with rat and
Mouse is as subjects, i.e., rat is as predator, and mouse is as prey.
The top view of the experimental provision of animal instinct fear behavior in the prior art is as shown in Figure 1, include for accommodating
The second experimental box 2' of mouse, for accommodating the first experimental box 1' of rat, and the experiment of the second experimental box 2' of connection and first
The junction of interface channel 3', the second experimental box 2' and the first experimental box 1' and interface channel 3' between case 1' are provided with use
In the opening 4' that mouse drills out.For mouse, the second experimental box 2' is safety zone, interface channel 3' and the first experimental box
1' is danger zone.It tests above the experimental provision of instinct fear behavior and is provided with camera system.
When test, mouse is recorded by camera system and distinguishes residence time, into secondary in danger zone and safety zone
Number, the number with rat close contact, quantify animal instinct fear reaction.
The experimental provision of test instinct fear behavior in the prior art has following problem:
(1) each experimental provision can only measure the instinct fear behavior of a mouse, need to prepare multiple experimental provisions with
More mouse are tested, experimental period is long, low efficiency；
(2) in the same experimental provision, more mouse is replaced and carry out many experiments, share same rat conduct every time
Predator, rat can be tired after many experiments, is unwilling to contact with mouse, influences the accuracy of experimental result；If replaced
Tired rat not only results in the waste of rat, increases experimental cost, and experimental variable also increases, same to influence experiment
As a result accuracy.
Summary of the invention
In view of the above-mentioned problems, a purpose of the invention is the experimental provision for proposing a kind of animal instinct fear behavior, it can
More preys are tested simultaneously, and use same predator, experimental efficiency is high.
Another object of the present invention is to propose a kind of experimental method of animal instinct fear behavior, and step is reasonable, experiment effect
Rate is high, and experimental result is more accurate.
The invention adopts the following technical scheme:
A kind of experimental provision of animal instinct fear behavior, including the first experimental box for accommodating predator, and setting
Camera system above the first experimental box, further includes:
Center experimental box in the centrally disposed experimental box of the first experimental box, is provided with multiple partitions in the experimental box of center, more
Space between first experimental box and center experimental box is divided into multiple active box by a partition；
Multiple second experimental boxs can accommodate prey in each second experimental box；
Multiple interface channels, each active box pass through an interface channel and are connected with one of them second experimental box,
And opening in communication is all arranged in the junction of interface channel and active box and the second experimental box.
As a preferred solution of the present invention, through-hole is offered on the side wall of the first experimental box, so that the first experimental box
Interior predator is contacted by through-hole with the prey in multiple active box.
As a preferred solution of the present invention, the side-wall material of center experimental box, the side-wall material of the second experimental box,
The material of the side-wall material of one experimental box, the material of interface channel and partition is non-transparent material.
As a preferred solution of the present invention, center experimental box is rectangular box, and the second experimental box, partition and connection are logical
There are four road is respectively provided with, the space between the first experimental box and center experimental box is divided into four active box by four partitions.
As a preferred solution of the present invention, center experimental box, the second experimental box, the first experimental box and interface channel
Top is non-close structure.
As a preferred solution of the present invention, center experimental box, the second experimental box, the first experimental box, partition and connection
The material in channel is stainless steel.
As a preferred solution of the present invention, the cube structure that center experimental box is 35 centimetres -45 centimetres of side length, the
The cube structure that two experimental boxs are 20 centimetres -30 centimetres of side length, the square that the first experimental box is 20 centimetres -30 centimetres of side length
Structure, interface channel are the cuboid of 50 centimetres -70 centimetres of length, 25 centimetres -35 centimetres of 10 centimetres -15 centimetres of width and height
Structure.
A kind of experimental method of animal instinct fear behavior, using above-mentioned experimental provision, comprising the following steps:
In the first experimental period, every above-mentioned prey is repeatedly correspondingly placed into second experimental box respectively
It is interior, enable every prey in corresponding second experimental box activity to adapt to the environment of corresponding second experimental box；
In the second experimental period, every above-mentioned prey is repeatedly correspondingly placed into second experimental box respectively
It is interior, enable every prey movable in the second experimental box, interface channel and active box of corresponding connection, to adapt to correspond to
The second experimental box, the environment in interface channel and active box；
In third experimental period, every above-mentioned prey is repeatedly correspondingly placed into second experimental box respectively
It is interior, a predator is put into the first experimental box, so that every prey is preyed in corresponding active box with same
Person forces contact, and records every prey in the behavior of safety zone and danger zone；
In the 4th experimental period, every above-mentioned prey is correspondingly placed into second experimental box respectively, is made
Obtaining every prey can be movable in the second experimental box, interface channel and active box of corresponding connection, and records every and be caught
Behavior of the trencherman in corresponding safety zone and danger zone.
As a preferred solution of the present invention, safety zone is in the second experimental box, and danger zone is active box and company
Connect in road, the behavior of record include prey be in safety zone and danger zone time, into or leave danger area
The number in domain and the number contacted with predator.
As a preferred solution of the present invention, the period of entire experimental method is 8 days；
First experimental period is the 1st day to the 3rd day, and every prey is correspondingly placed into one second experiment respectively daily
Activity 20 minutes in case enable the activity in corresponding second experimental box of every prey to test to adapt to corresponding second
The environment of case；
It is the 4th day to the 6th day in second experimental period, every above-mentioned prey is correspondingly placed into one respectively daily
Activity 20 minutes in second experimental box enable every prey in the second experimental box, interface channel and the work of corresponding connection
Activity in dynamic case, to adapt to the environment in corresponding second experimental box, interface channel and active box；
Third experimental period is the 7th day, and every above-mentioned prey is correspondingly placed into second experimental box respectively
It freely explores 10 minutes, a predator is put into the first experimental box, so that every prey is in corresponding active box
It forces contact 5 seconds with same predator, every prey is forced to be recorded in 10 minutes after contacting corresponding
The behavior of safety zone and danger zone；
4th experimental period is the 8th day, and every above-mentioned prey is correspondingly placed into second experimental box respectively,
Enable every prey movable in the second experimental box, interface channel and active box of corresponding connection, and records every quilt
Behavior of the predator in 10 minutes intrinsic safety regions and danger zone.
It as a preferred solution of the present invention, further include light stimulus experiment, light stimulus experimental setup is when third is tested
In, every prey is recorded in 10 minutes in corresponding safety zone and danger after being specifically located at pressure contact
It is carried out after the behavior in region.
The invention has the benefit that
A kind of experimental provision of animal instinct fear behavior proposed by the present invention, by the way that center experimental box is arranged, center is real
Multiple partitions are provided in tryoff, the space between the first experimental box and center experimental box is divided into multiple activities by multiple partitions
Case, each second experimental box pass through interface channel and are connected to corresponding active box, so that more in multiple second experimental boxs
Prey can be contacted with same predator simultaneously in multiple active box, so that more preys generate fear, because
This is with one prey of the prior art compared with the device that a predator contacts, hence it is evident that improves conventional efficient.
The experimental method of a kind of animal instinct fear behavior proposed by the present invention, using above-mentioned experimental provision, in four realities
It tests in the time, realizes the experiment to animal instinct fear behavior, step is reasonable, and experimental efficiency is high, and experimental result is more accurate.
Detailed description of the invention
Fig. 1 is the top view of the experimental provision of animal instinct fear behavior in the prior art；
Fig. 2 is the top view of the experimental provision of animal instinct fear behavior provided by the invention.
In figure:
1, the first experimental box；2, the second experimental box；3, interface channel；4, it is open；5, center experimental box；6, partition；7, living
Dynamic case；
1', the first experimental box；2', the second experimental box；3', interface channel；4', opening.
Specific embodiment
The present invention is described in further detail with reference to the accompanying drawings and examples.It is understood that this place is retouched
The specific embodiment stated is used only for explaining the present invention rather than limiting the invention.It also should be noted that in order to just
Only the parts related to the present invention are shown in description, attached drawing rather than entire infrastructure.
In the present invention unless specifically defined or limited otherwise, fisrt feature second feature "upper" or "lower"
It may include that the first and second features directly contact, also may include that the first and second features are not direct contacts but pass through it
Between other characterisation contact.Moreover, fisrt feature includes the first spy above the second feature " above ", " above " and " above "
Sign is right above second feature and oblique upper, or is merely representative of first feature horizontal height higher than second feature.Fisrt feature exists
Second feature " under ", " lower section " and " following " include that fisrt feature is directly below and diagonally below the second feature, or is merely representative of
First feature horizontal height is less than second feature.
Fig. 2 is the top view of the experimental provision of animal instinct fear behavior provided by the invention, as shown in Fig. 2, main packet
It includes: the camera system (not shown) above the first experimental box is set, multiple second experimental boxs 2, the first experimental box 1, in
Heart experimental box 5 and multiple interface channels 3.Wherein for the first experimental box 1 for accommodating predator, the first experimental box 1 is centrally disposed
In experimental box 5, be provided with multiple partitions 6 in center experimental box 5, multiple partitions 6 by the first experimental box 1 and center experimental box 5 it
Between space be divided into multiple active box 7, prey can be accommodated in each second experimental box 2, each active box 7 passes through one
A interface channel 3 is connected with one of them second experimental box 2, and the company of interface channel 3 and active box 7 and the second experimental box 2
The place of connecing all is arranged opening 4 and is connected.This set enables the predator in the first experimental box 1 shared, and each second is real
Prey in tryoff 2 can be drilled out from opening 4, by the interface channel 3 of corresponding connection, pierce active box 7 from opening 4
It is interior, the predator of the first experimental box 1 is encountered, after generating the behavior of instinct fear, it is real that prey can be back to corresponding second
In tryoff 2.Therefore, in the experimental provision of animal instinct fear behavior provided by the invention, same predator enables to more
Prey generate it is frightened, therefore with one prey of the prior art compared with the device that a predator contacts, hence it is evident that
Improve conventional efficient.
Specifically, as shown in Figure 2, preferred center experimental box 5 is rectangular box, the second experimental box 2, partition 6 and connection
There are four channel 3 is respectively provided with, the space between the first experimental box 1 and center experimental box 5 is divided into four activities by four partitions 6
Case 7, i.e. four active box 7 are correspondingly arranged at four side-walls of center experimental box 5.I.e. such as the experimental provision in Fig. 2, at most may be used
To test simultaneously to four preys, conventional efficient is improved.It is anticipated that ground is, the present invention does not limit center reality
The number of the shape of tryoff 5 and the second experimental box 2, partition 6 and interface channel 3, if can simultaneously to more preys into
Row experiment, within the scope of protection of this application.Preferred center experimental box 5 of the present invention be rectangular box, the second experimental box 2, every
There are four plate 6 and interface channel 3 are respectively provided with, this set makes be divided into four active box 7, is capable of providing sufficient space
For frightened behavior occurs for prey and turns to and returns to the second experimental box 2, while each cabinet is more preferably processed and is assembled.In this reality
It applies in example, opening 4, and the size of the opening 4 of the second experimental box 2 is provided on each second experimental box 2 and each active box 7
Prey is enabled to freely to pierce or drill out with the size for the opening 4 being arranged on active box 7.
Further, in order to which the predator realized in the first experimental box 1 can connect with the prey in multiple active box 7
Touching, offers through-hole on the side wall of the first experimental box 1.Predator's energy in contact not just the first experimental box 1 herein
See the prey in multiple active box 7, but predator is possible to actually touch prey.That is the first experimental box 1
Material be only transparent material, enable the predator in the first experimental box 1 to see that the prey in multiple active box 7 is
Not all right, only predator is possible to actually touch prey, and prey could be made really to generate instinct fear row
For.
Specifically, the material of the first experimental box 1, transparent material or non-transparent material are not limited, in the first experimental box 1
Side wall on offer through-hole, predator can directly contact from these through-holes with prey.It can also the preferably first reality
Tryoff 1 is the cage structure formed by grid or net, by taking prey is mouse and predator is rat as an example, the first experimental box
Through-hole is offered on 1 side wall or the first experimental box 1 is arranged to cage structure, so that the rat in the first experimental box 1 can not be from
It is drilled out in the gap of through-hole or cage structure, but the nose of rat, mouth or claw can be out of, through-hole or cage structure gaps
It stretches out, the mouse in active box 7 is directly contacted, the instinct fear behavior of mouse is caused.The specific structure of first experimental box 1 exists
This is not further limited, as long as the structure of the first experimental box 1 can enable predator and multiple work in the first experimental box 1
Prey contact in dynamic case 7 is within the scope of the invention.
Further, the side-wall material of center experimental box 5, the side-wall material of the second experimental box 2, the first experimental box 1 side
The material of wall material, the material of interface channel 3 and partition 6 is non-transparent material.This set makes in the second experimental box 2
Prey can not see other prey, avoid when movable in the second experimental box 2, interface channel 3 and active box 7
Behavior act between more preys generates mutually interference.Center experimental box 5, the second experimental box 2, the first experimental box 1, every
The material of plate 6 and interface channel 3 is preferably stainless steel.Further, in order to realize that the camera system above experimental provision can
The action situation of multiple preys and predator in experimental provision is accurately captured and records, center experimental box 5, second is real
The top of tryoff 2, the first experimental box 1 and interface channel 3 is non-close structure.I.e. each experimental box and channel are no top cover knot
Structure, the camera system above such experimental provision shoot downwards, just can accurately capture and record more in experimental provision
The action situation of a prey and predator
In the present invention, it is preferred to protect the following size of each cabinet.Center experimental box 5 is 35 centimetres -45 centimetres of side length
Cube structure, preferably 40 centimetres * 40 centimetres * 40 centimetres of cube structure；Second experimental box 2 is 20 centimetre -30 of side length
Centimetre cube structure, the first experimental box 1 is 20 centimetres -30 centimetres of side length of cube structure, preferably 25 centimetres * 25 lis
The cube structure of * 25 centimetres of rice；Interface channel 3 is 50 centimetres -70 centimetres of length, 10 centimetres -15 centimetres of width and height 25
Centimetres -35 centimetres of rectangular parallelepiped structure, preferably 60 centimetres * 12.5 centimetres * 30 centimetres of rectangular parallelepiped structure；The width of opening 4 is 1
It is centimetres -3 centimetres, preferably 2 centimetres wide.This structure size setting, according to predator and can be caught by those skilled in the art
What the type and body size of trencherman was configured, and the above-mentioned size that the present invention preferably protects, it is to be obtained by experiment, needle
It is rat to predator, prey is a kind of reasonable size of mouse.
The present invention also protects a kind of experimental method of animal instinct fear behavior, it is preferred to use above-mentioned experimental provision, it is main
Want the following steps are included:
In the first experimental period, every prey is repeatedly correspondingly placed into second experimental box respectively, so that
Every prey can in corresponding second experimental box activity to adapt to the environment of corresponding second experimental box；
In the second experimental period, every above-mentioned prey is repeatedly correspondingly placed into second experimental box respectively
It is interior, enable every prey movable in the second experimental box, interface channel and active box of corresponding connection, to adapt to correspond to
The second experimental box, the environment in interface channel and active box；
In third experimental period, every above-mentioned prey is repeatedly correspondingly placed into second experimental box respectively
It is interior, a predator is put into the first experimental box, so that every prey is preyed in corresponding active box with same
Person forces contact, and records every prey in the behavior of safety zone and danger zone；
In the 4th experimental period, every above-mentioned prey is correspondingly placed into second experimental box respectively, is made
Obtaining every prey can be movable in the second experimental box, interface channel and active box of corresponding connection, and records every and be caught
Behavior of the trencherman in corresponding safety zone and danger zone.
By above-mentioned steps it is found that the first experimental period and the second experimental period are time, enable prey
The environment of entire experimental provision is adapted to, influence of the additional variable to experiment accuracy is reduced；Third experimental period is to carry out instinct
The time of frightened behavioral experiment is analyzed, the data quantified by the behavior to prey in third experimental period,
It can be obtained by the data of prey instinct fear behavior；4th experimental period is the testing time after experiment, for analyzing
After prey generates the behavior of instinct fear, continue the influence to prey.
Further, the present invention protects specific experimental method below, specifically includes that
Firstly, using above-mentioned experimental provision, it can be deduced that, it is safety in the second experimental box for prey
It is danger zone in region, active box and interface channel.The behavior of record includes that prey is in safety zone and danger
The time in region, into or the number that leaves the number of danger zone and contacted with predator, the data of these quantizations are by testing
Camera system record above device.Specifically, camera system includes the control of at least one video camera and corresponding electrical connection
System and analysis software, by the video input of shooting into analysis software, analysis software can identify and quantify above-mentioned video camera
The data of behavior can study animal instinct fear behavior by arranging and analyzing these quantized datas.
Specifically, the period of the preferably entire experimental method of the present invention is 8 days, and by mouse of prey and predator is big
For mouse, comprising:
First experimental period is the 1st day to the 3rd day.On day 1 in the 3rd day, 4 mouse are correspondingly placed into respectively daily
Activity 20 minutes, enable the activity in corresponding second experimental box of every mouse corresponding to adapt in 4 the second experimental boxs
The environment of second experimental box.In this course, a block baffle can be placed to block in the opening of each second experimental box
Mouse realizes that mouse is only movable in the second experimental box.
It is the 4th day to the 6th day in second experimental period.On day 4 in the 6th day, daily respectively by 4 above-mentioned mouse
Activity 20 minutes in 4 the second experimental boxs are correspondingly placed into, the gear that the opening of the second experimental box each in previous step is placed
Plate is removed, and enables the activity in the second experimental box, interface channel and active box of corresponding connection of every mouse, i.e. mouse can be with
It pierces or drills out from opening, to adapt to the environment in corresponding second experimental box, interface channel and active box.In fact due to second
Tryoff, interface channel, partition and active box are nontransparent material, therefore, be can't see mutually between more mouse, will not be to reality
Test generation interference.
Third experimental period is the 7th day, this process specifically includes:
4 above-mentioned mouse are correspondingly placed into 4 the second experimental boxs, in each second experimental box by the first step respectively
The baffle that opening is placed allows mouse freely to explore in corresponding second experimental box 10 minutes to block mouse；Simultaneously
One rat is put into the first experimental box.
Second step removes the baffle that the opening of each second experimental box is placed, due on day 4 in the 6th day, mouse
It already is familiar with movable route, therefore mouse can enter interface channel across opening and enter back into active box, this mistake
Journey is the free action process of mouse.
Third step places baffle in the opening of 4 active box, so that 4 when four mouse enter in active box
Mouse is forced to contact 5 seconds in corresponding active box with same rat.It is internal at this time due to the structure of the first experimental box
Rat can generate with every mouse and directly contact, excite the instinct fear of 4 mouse.
After 5 seconds, the baffle that the opening of 4 active box is placed is removed for 4th step, for 4 for generating instinct fear
Mouse provides the channel escaped, at this point, being recorded in 4 mouse in 10 minutes in corresponding safety zone and danger zone
Behavior.That is, the rat inside the first experimental box is not taken out in the 4th step, therefore for every mouse, interface channel
With the presence of rat can be experienced in active box, therefore danger zone is belonged in interface channel and active box, and second is real
The presence that rat cannot be experienced in tryoff belongs to safety zone in the second experimental box for mouse.In 10 minutes, 4
Mouse may be all in the second experimental box, it is also possible to have some animals in interface channel, it is also possible to have some animals in activity
In case, therefore the instinct fear degree of corresponding every mouse is just different.If mouse is got in the time that danger zone is waited for
Long, the number into danger zone is more, more with the number of rat close contact, and it is lower to represent its instinct fear；It is on the contrary
?.
Further, in above-mentioned 7th day the 4th step, rat has stopped 10 minutes 5 altogether inside the first experimental box
Second, the behavior of instinct fear is generated to 4 mouse.If using experimental provision in the prior art as shown in Figure 1,1 mouse
It can only be tested with 1 rat, if it is desired to 4 mouse are realized, then rat will contact with 4 mouse respectively,
4 mouse are made to generate the behavior of instinct fear.Therefore, in the prior art, if it is desired to share a rat to accelerate conventional efficient,
Same rat is most short to need to test 20 seconds 40 minutes, and rat is easy to tired, is unwilling to contact with mouse, influences experimental result
Accuracy；If replacing tired rat, the waste of rat is not only resulted in, increases experimental cost, and experimental variable
Also increase, the same accuracy for influencing experimental result.Therefore, disclosed by the invention compared with experimental provision in the prior art
Experimental provision is able to use same predator, while testing to more preys, and experimental efficiency is high.
4th experimental period is the 8th day, 4 above-mentioned mouse is correspondingly placed into 4 the second experimental boxs respectively, so that often
Mouse can the activity in the second experimental box, interface channel and active box of corresponding connection, and record every mouse at 10 minutes
The behavior in intrinsic safety region and danger zone.During this, rat, therefore every are not placed inside the first experimental box
The behavior of mouse is that the behavior after generating instinct fear at the 7th day can be used as by recording the behavior of every mouse at this time
Evaluate the data of mouse instinct fear behavior.
Further, for above-mentioned each experimental procedure, it is also necessary to control the variable of other influences data accuracy, such as
Temperature, humidity, noise and illumination of environment etc..In order to guarantee each experimental procedure, mouse not will receive a mouse gas
The influence of taste, after each step, all it is preferable to use 25% alcohol carries out disinfection processing to experimental provision.
Further, the experimental provision of animal instinct fear behavior proposed by the present invention can also carry out light stimulus reality simultaneously
It tests, i.e. light stimulus experiment and the experiment of animal instinct fear can be used in conjunction with a set of experimental provision.It is real when needing to carry out light stimulus
When testing, light stimulus experimental procedure can be arranged and be carried out after the 4th step at the 7th day.It specifically includes and gives mouse 10 minutes light
Stimulation, re-records every mouse in the behavior in 10 minutes intrinsic safety regions and danger zone after light stimulus.
Since light stimulus experiment and the experiment of animal instinct fear are identical entire experimental period, it can be same at the 7th day
Shi Jinhang, which offers a saving the experimental periods of light stimulus experiment, improve conventional efficient.It is anticipated that ground is, the present invention is simultaneously
Light stimulus experiment itself is not limited, as long as it is identical to test entire experimental period with animal instinct fear, and can share a set of reality
Other experiments of experiment device, can be arranged in the experimental period of animal instinct fear experiment.
Note that the above is only a better embodiment of the present invention and the applied technical principle.It will be appreciated by those skilled in the art that
The present invention is not limited to specific embodiments here, be able to carry out for a person skilled in the art it is various it is apparent variation, again
Adjustment and substitution are without departing from protection scope of the present invention.Therefore, although by above embodiments to the present invention carried out compared with
For detailed description, but the present invention is not limited to the above embodiments only, without departing from the inventive concept, can be with
Including more other equivalent embodiments, and the scope of the invention is determined by the scope of the appended claims.
Claims (11)
1. a kind of experimental provision of animal instinct fear behavior, including the first experimental box (1) for accommodating predator, and setting
Camera system above first experimental box (1), which is characterized in that further include:
Center experimental box (5), the first experimental box (1) setting is in the center experimental box (5), the center experimental box
(5) it is provided with multiple partitions (6) in, multiple partitions (6) are by first experimental box (1) and the center experimental box (5)
Between space be divided into multiple active box (7)；
Multiple second experimental boxs (2), each second experimental box (2) is interior to accommodate prey；
Multiple interface channels (3), each active box (7) is by described in an interface channel (3) and one of them
Second experimental box (2) is connected, and the connection of the interface channel (3) and the active box (7) and second experimental box (2)
Place's all setting openings (4) are connected.
2. the experimental provision of animal instinct fear behavior according to claim 1, which is characterized in that first experimental box
(1) through-hole is offered on side wall, so that the predator in first experimental box (1) passes through the through-hole and multiple work
Prey contact in dynamic case (7).
3. the experimental provision of animal instinct fear behavior according to claim 1, which is characterized in that the center experimental box
(5) side-wall material, the side-wall material of second experimental box (2), the side-wall material of first experimental box (1), the company
The material of the material and the partition (6) of connecting road (3) is non-transparent material.
4. the experimental provision of animal instinct fear behavior according to claim 1 to 3, which is characterized in that described
Center experimental box (5) is rectangular box, and second experimental box (2), the partition (6) and the interface channel (3) are respectively provided with
There are four, the space between first experimental box (1) and the center experimental box (5) is divided by four partitions (6)
Four active box (7).
5. the experimental provision of animal instinct fear behavior according to claim 1 to 3, which is characterized in that described
It is above center experimental box (5), second experimental box (2), first experimental box (1) and the interface channel (3)
Non-close structure.
6. the experimental provision of animal instinct fear behavior according to claim 1 to 3, which is characterized in that described
Center experimental box (5), second experimental box (2), first experimental box (1), the partition (6) and the interface channel
(3) material is stainless steel.
7. the experimental provision of animal instinct fear behavior according to claim 4, which is characterized in that the center experimental box
(5) cube structure for being 35 centimetres -45 centimetres of side length, the pros that second experimental box (2) is 20 centimetres -30 centimetres of side length
Body structure, the cube structure that first experimental box (2) is 20 centimetres -30 centimetres of side length, the interface channel (3) are length
50 centimetres -70 centimetres, 10 centimetres -15 centimetres of width and highly 25 centimetres -35 centimetres of rectangular parallelepiped structure.
8. a kind of experimental method of animal instinct fear behavior, using experimental provision described in claim 1-7 any one,
It is characterized in that, comprising the following steps:
In the first experimental period, every prey is repeatedly correspondingly placed into second experimental box respectively, so that every
Prey can in corresponding second experimental box activity to adapt to the environment of corresponding second experimental box；
In the second experimental period, every above-mentioned prey is repeatedly correspondingly placed into second experimental box respectively, is made
Every prey can the activity in the second experimental box, interface channel and active box of corresponding connection, to adapt to corresponding the
Environment in two experimental boxs, interface channel and active box；
In third experimental period, every above-mentioned prey is repeatedly correspondingly placed into second experimental box respectively, it will
One predator is put into the first experimental box, so that every prey is forced in corresponding active box with same predator
Contact, and every prey is recorded in the behavior of safety zone and danger zone；
In the 4th experimental period, every above-mentioned prey is correspondingly placed into second experimental box respectively, so that often
Prey can the activity in the second experimental box, interface channel and active box of corresponding connection, and record every prey
Behavior in corresponding safety zone and danger zone.
9. the experimental method of animal instinct fear behavior according to claim 8, which is characterized in that the safety zone is
In second experimental box, the danger zone is in active box and interface channel；The behavior of record includes that prey is in safety
The time of region and danger zone, into or the number that leaves the number of danger zone and contacted with predator.
10. the experimental method of animal instinct fear behavior according to claim 9, which is characterized in that
The period of entire experimental method is 8 days；
First experimental period is the 1st day to the 3rd day, and every prey is correspondingly placed into second experimental box respectively daily
Activity 20 minutes, enable every prey in corresponding second experimental box activity to adapt to corresponding second experimental box
Environment；
It is the 4th day to the 6th day in second experimental period, it is above-mentioned respectively daily that every prey is correspondingly placed into one second
Activity 20 minutes in experimental box enable every prey in the second experimental box, interface channel and the active box of corresponding connection
Interior activity, to adapt to the environment in corresponding second experimental box, interface channel and active box；
Third experimental period is the 7th day, is respectively correspondingly placed into every above-mentioned prey in one the second experimental box freely
It explores 10 minutes, a predator is put into the first experimental box, so that every prey is in corresponding active box and together
One predator forces contact 5 seconds, forces to be recorded in after contact in 10 minutes every prey in corresponding safety
The behavior in region and danger zone；
4th experimental period is the 8th day, and every above-mentioned prey is correspondingly placed into second experimental box respectively, so that
Every prey can be movable in the second experimental box, interface channel and active box of corresponding connection, and records every prey
Behavior of the person in 10 minutes intrinsic safety regions and danger zone.
11. the experimental method of animal instinct fear behavior according to claim 10, which is characterized in that further include light stimulus
Experiment, the light stimulus experimental setup are recorded in 10 minutes often after being specifically located at pressure contact in third experimental period
Prey carries out after the behavior of corresponding safety zone and danger zone.
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