CN110278884B - Detection device and detection method for animal instinct fear behavior - Google Patents
Detection device and detection method for animal instinct fear behavior Download PDFInfo
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- CN110278884B CN110278884B CN201910514494.8A CN201910514494A CN110278884B CN 110278884 B CN110278884 B CN 110278884B CN 201910514494 A CN201910514494 A CN 201910514494A CN 110278884 B CN110278884 B CN 110278884B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K1/00—Housing animals; Equipment therefor
- A01K1/02—Pigsties; Dog-kennels; Rabbit-hutches or the like
- A01K1/03—Housing for domestic or laboratory animals
- A01K1/031—Cages for laboratory animals; Cages for measuring metabolism of animals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K29/00—Other apparatus for animal husbandry
- A01K29/005—Monitoring or measuring activity, e.g. detecting heat or mating
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- H—ELECTRICITY
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- H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
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Abstract
The invention provides a detection device and a detection method for animal instinct fear behaviors, wherein the detection device for the animal instinct fear behaviors comprises the following steps: the animal shelter device comprises a box body, a shelter and a control system, wherein the box body comprises a behavior test box for an animal to perform instinct fear behavior test and a shelter box for the animal to shelter after instinct fear reaction, and an opening for communicating the behavior test box and the shelter box is arranged between the behavior test box and the shelter box; the visual stimulation device is used for sending a stimulation signal to the animal so as to stimulate the animal to make instinct fear response; and the camera is arranged above the box body and used for recording the behavioral response of the animal. The detection device and the detection method for the animal instinct fear behavior can accurately acquire the movement path and the movement speed of the animal, and effectively improve the reliability and the stability of the experimental result.
Description
Technical Field
The invention belongs to the technical field of animal behaviors, and particularly relates to a detection device and a detection method for animal instinct fear behaviors.
Background
Fear is one of six basic emotional behaviors, and is induced by the perception of danger by a subject, and the defense behavior generated by fear is an important guarantee for the survival and the multiplication of animals and is the most important and conserved part in the species evolution process.
Disorder of fear regulation may lead to a series of related diseases, such as panic disorder and specific phobia. The research on the instinct fear loop can help to find the occurrence mechanism of the mental diseases and develop a new diagnosis and treatment strategy. Animal models for loop studies, particularly mouse models, are widely used to study mental disease, and for mice, avoidance of air predators, such as eagle, is an important viability that is likely to be mediated by specific neural loops.
The device for researching the instinct fear behavior of the mouse in the laboratory at present is usually used for placing a shielding object in a box, so that the mouse is difficult to shoot the behavior of the mouse after escaping into the shielding object.
Disclosure of Invention
The invention aims to provide a detection device for animal instinct fear behaviors, which aims to solve the technical problem that the movement path of an animal cannot be accurately acquired in the prior art.
In order to achieve the purpose, the invention adopts the technical scheme that: provided is an animal instinct fear behavior detection device, including:
the animal shelter device comprises a box body, a shelter and a control system, wherein the box body comprises a behavior test box for an animal to perform instinct fear behavior test and a shelter box for the animal to shelter after instinct fear reaction, and an opening for communicating the behavior test box and the shelter box is arranged between the behavior test box and the shelter box;
the visual stimulation device is used for sending a stimulation signal to the animal so as to stimulate the animal to make instinct fear response; and the number of the first and second groups,
the camera is arranged above the box body and used for recording the behavioral response of the animal.
Further, the cross-sectional shape of the side wall of the behavior test box is circular or elliptical.
Further, the visual stimulation device is arranged above or on the periphery of the behavior test box.
Further, the visual stimulation device is a display screen.
Further, the camera is disposed above the opening.
Further, animal instinct fear action detection device still includes the baffle, it is kept away from along keeping away from the case sets up a plurality of screens that are used for fixing at the direction interval of action test box the baffle, baffle demountable installation is in arbitrary one first screens.
Further, the lateral margin of the opening is provided with a second clamping position for fixing the partition plate.
Further, the animal instinct fear behavior detection device further comprises a support used for supporting the visual stimulation device and the camera, and the support is arranged outside the box body.
Further, the periphery of the behavior test box is provided with an outer wall for shading light and insulating sound.
The invention also provides a detection method of the animal instinct fear behavior, which comprises the following steps:
placing an animal in a box of the animal instinct fear behavior detection device as described in any one of the above;
the visual stimulation device sends a stimulation signal to the animal, and the animal makes an instinct fear response after receiving the stimulation signal;
the camera records the behavioral response of the animal.
The detection device and the detection method for the instinct fear behavior of the air-conditioned animal have the beneficial effects that: compared with the prior art, the animal instinct fear behavior detection device is provided with the behavior test box and the avoiding box, the opening for communicating the behavior test box with the avoiding box is arranged between the behavior test box and the avoiding box, the camera can be arranged above the box body, and the camera can shoot from the upper side, so that the shot picture shape is reduced, the motion path and the motion speed of an animal can be accurately obtained, the reliability and the stability of an experimental result are effectively improved, and the problem that the shooting picture is deformed due to the fact that the camera can only shoot from the side surface because a shielding object is arranged in the box body in the prior art, and the exact motion path of the animal cannot be obtained is avoided.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the embodiments or the prior art descriptions will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without inventive exercise.
Fig. 1 is a schematic perspective view of an apparatus for detecting an animal instinct fear behavior according to an embodiment of the present invention;
fig. 2 is a schematic perspective view of a box according to an embodiment of the present invention;
fig. 3 is a schematic perspective view of an apparatus for detecting an animal instinct fear behavior according to another embodiment of the present invention.
Wherein, in the figures, the respective reference numerals:
10-a box body; 11-a behavioral testing box; 12-avoidance box; 13-opening; 20-a camera; 30-a separator; 40-a scaffold; 50-outer wall.
Detailed Description
In order to make the technical problems, technical solutions and advantageous effects to be solved by the present invention more clearly apparent, the present invention is further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
It will be understood that when an element is referred to as being "secured to" or "disposed on" another element, it can be directly on the other element or be indirectly on the other element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or be indirectly connected to the other element.
It will be understood that the terms "length," "width," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like, as used herein, refer to an orientation or positional relationship indicated in the drawings that is solely for the purpose of facilitating the description and simplifying the description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and is therefore not to be construed as limiting the invention.
Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless specifically defined otherwise.
Referring to fig. 1 and fig. 2 together, the device for detecting the animal instinct fear behavior according to the present invention will now be described. The invention provides a device for detecting the instinct fear behavior of animals, which comprises a box body 10, a visual stimulation device (not shown) and a camera 20. The visual stimulation device is used for sending a stimulation signal to the animal so as to stimulate the animal to make instinct fear response. In this example, mice were selected for the test. A camera 20 is provided above the housing 10 for recording behavioral responses of the animal. The box body 10 comprises a behavior test box 11 and an avoiding box 12, and the behavior test box 11 is used for the animal to carry out instinct fear behavior test. The avoidance box 12 is used for the animal to avoid after making instinct fear reaction. An opening 13 used for communicating the behavior test box 11 with the avoidance box 12 is arranged between the behavior test box 11 and the avoidance box 12, and when the animal is stimulated by the visual stimulation device, the animal can make instinct fear reaction, so that the animal enters the avoidance box 12 through the opening 13 to avoid. According to the invention, the behavior test box 11 and the avoidance box 12 are arranged separately, so that the camera 20 can shoot from the upper part, the shot picture is clear, and the situation that the shot picture is deformed due to the fact that the camera 20 can only shoot from the side surface because a shelter is arranged in the box body 10 in the prior art, and the exact motion path of an animal cannot be obtained is avoided.
Compared with the prior art, the animal instinct fear behavior detection device provided by the invention has the advantages that the behavior test box 11 and the avoidance box 12 are arranged, the opening 13 for communicating the behavior test box 11 with the avoidance box 12 is arranged between the behavior test box 11 and the avoidance box 12, the camera 20 can be arranged above the box body 10, the camera 20 can shoot from the upper side, the shot picture shape is reduced, the motion path and the motion speed of an animal can be accurately obtained, the reliability and the stability of an experimental result are effectively improved, and the problem that the camera 20 can only shoot from the side surface to cause the deformation of the shot picture due to the fact that a shielding object is arranged in the box body 10 in the prior art is avoided.
Further, referring to fig. 1 and 2, as an embodiment of the device for detecting the instinct fear behavior of an animal provided by the present invention, the side wall of the behavior test box 11 has a circular cross-sectional shape. Because the behavior testing device of the traditional technology usually adopts a cuboid or a cube box with four corners, the corners of the mouse can be considered as safe regions when the mouse faces the shadow of the simulated natural enemy, thereby introducing uncertain factors for the output of the instinctive fear behavior and leading to inaccurate detection results. The cross section of the side wall of the behavior test box 11 is round, so that the problem of corners is solved, the stability and reliability of the experiment are improved, and the problem that in the prior art, the corners of the side walls are easy to be regarded as safe areas by animals due to the fact that the corners exist among the side walls at the periphery because a square box body is adopted is solved, and the detection result is inaccurate. It should be noted that the cross-sectional shape of the side wall of the behavior test box 11 is not limited to this, for example, in other preferred embodiments of the present invention, the cross-sectional shape of the side wall of the behavior test box 11 may also be an ellipse.
Further, as a specific embodiment of the device for detecting the animal instinct fear behavior provided by the present invention, the visual stimulation device is disposed above the behavior test box 11, so that the response of the mouse to the visual danger stimulation of the upper visual field can be detected. The stimulation signal emitted by the visual stimulation device may be a black disc. It should be noted that the cross-sectional shape of the side wall of the behavior test box 11 is not limited thereto, for example, in other preferred embodiments of the present invention, the visual stimulation device may be disposed on the peripheral side of the behavior test box 11 so as to detect the behavior response of the mouse to the visual stimulation from the periphery. Preferably, the visual stimulation means is a display screen. The black disc is shown by using a display screen.
Further, referring to fig. 1 and 2, as an embodiment of the device for detecting the instinct fear behavior of an animal provided by the present invention, a camera 20 is disposed above the opening 13. By installing the camera 20 above the opening 13, the camera 20 can photograph the entire behavior test box 11 and the avoidance box 12, and there is no dead angle in photographing. Preferably, the camera 20 is a wide-angle camera. In order to avoid that the visual stimulation means blocks the shot to the camera 20, the height of the camera 20 should be lower than the height at which the visual stimulation means is located.
Further, referring to fig. 1 and fig. 2, as a specific embodiment of the device for detecting the instinct fear behavior of the animal provided by the present invention, the device for detecting the instinct fear behavior of the animal further includes a partition plate 30, the hiding box 12 is provided with a plurality of first blocking positions (not shown) at intervals along a direction away from the behavior testing box 11, wherein the direction away from the behavior testing box 11 of the hiding box 12 refers to a length direction of the hiding box 12 in this embodiment, the first blocking positions can be used for fixing the partition plate 30, and the partition plate 30 can be detachably mounted in any one of the first blocking positions, so that the hiding box 12 can be divided into different lengths, and the length of the hiding box 12 can be changed according to actual needs. Specifically, the first clamping position can be a concave position arranged on the side wall of the shelter box, and the partition plate is only required to be placed in the concave position, so that the operation is very simple and convenient.
Further, referring to fig. 1 and 2, as an embodiment of the device for detecting the instinct fear behavior of the animal provided by the present invention, a second detent (not shown) is provided at a side edge of the opening 13, and the second detent is used for fixing the partition 30. When the partition board 30 is clamped on the second clamping position, the hiding box 12 is separated from the behavior testing box by the partition board 30, and the mouse can only move in the behavior testing box 11 and can not enter the hiding box 12, so that the situation of the hiding box 12 can be compared.
Further, as a specific embodiment of the device for detecting the instinct fear behavior of the animal provided by the present invention, the device for detecting the instinct fear behavior of the animal further comprises a bracket 40 for supporting the visual stimulation device and the camera 20, wherein the bracket 40 is disposed outside the box body 10, and the visual stimulation device and the camera 20 can be fixed above the box body 10 by the arrangement of the bracket 40.
Further, referring to fig. 3, as a specific embodiment of the device for detecting the instinct fear behavior of an animal provided by the present invention, an outer wall 50 is disposed at the periphery of the behavior test box 11, and the outer wall 50 is used for shading light and insulating sound, so that the experimental conditions are stable.
Further, referring to fig. 1, as a specific embodiment of the device for detecting the instinct fear behavior of an animal provided by the present invention, the height of the box 10 is 25cm to 35cm, preferably 30cm, so that not only can the escape of the mouse be effectively prevented, but also the mouse can be ensured to have a sufficiently wide upper visual field. The behavioural test chamber 11 has a diameter of 45cm to 55cm, preferably 50 cm. The shelter box 12 is a rectangular parallelepiped structure, and the length of the shelter box 12 is 45cm-55cm, and the width is 5cm-15cm, for example, in one embodiment of the present invention, the length of the shelter box 12 is 50cm, and the width is 10 cm.
Further, as a specific implementation manner of the device for detecting the animal instinct fear behavior provided by the invention, the box body 10 is made of an acrylic plate, and acrylic has the advantages of light weight, low price, no toxicity, easiness in forming, good acid and alkali resistance and the like. Of course, the material of the box 10 is not limited to this, for example, in other preferred embodiments of the present invention, the box 10 may also be a thin metal plate. Specifically, the color of the box 10 may be changed according to the hair color of the mouse under study, for example, when the hair color of the mouse is black, the color of the bottom plate of the box 10 may be white, for example, the bottom plate may have a white frosted effect, and when the hair color of the mouse is white, the color of the bottom plate of the box 10 may be black, so as to achieve the best shooting effect. Preferably, when the behavior of the mouse needs to be recorded by shooting from the side, a side plate made of transparent acrylic can be adopted, so that the motion behavior of the mouse can be recorded from the periphery by a camera conveniently.
The invention also provides a detection method of the animal instinct fear behavior, which comprises the following steps:
and S10, placing the animal in the box body 10 of the animal instinct fear behavior detection device.
S20, the visual stimulation device sends out a stimulation signal to the animal, and the animal makes instinct fear response after receiving the stimulation signal.
S30, camera 20 records behavioral responses of the animal.
According to the invention, the behavior test box 11 and the avoiding box 12 are arranged, and the opening 13 for communicating the behavior test box 11 with the avoiding box 12 is arranged between the behavior test box 11 and the avoiding box 12, so that the camera 20 can be arranged above the box body 10, the camera 20 can shoot from the upper side, the shape of a shot picture is reduced, the motion path and the motion speed of an animal can be accurately obtained, the reliability and the stability of an experimental result are effectively improved, and the problem that the exact motion path of the animal cannot be obtained due to the fact that the shot picture is deformed because the camera 20 can only shoot from the side surface because a shielding object is arranged in the box body 10 in the prior art is avoided.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.
Claims (7)
1. Animal instinct fear action detection device, its characterized in that: the method comprises the following steps:
the animal shelter device comprises a box body, a shelter and a control system, wherein the box body comprises a behavior test box for an animal to perform instinct fear behavior test and a shelter box for the animal to shelter after instinct fear reaction, and an opening for communicating the behavior test box and the shelter box is arranged between the behavior test box and the shelter box;
the visual stimulation device is used for sending a stimulation signal to the animal so as to stimulate the animal to make instinct fear response; and the number of the first and second groups,
the camera is arranged above the box body and used for recording the behavioral response of the animal;
the cross section of the side wall of the behavior test box is circular or elliptical;
the animal instinct fear behavior detection device further comprises a partition plate, the avoidance box is provided with a plurality of first clamping positions for fixing the partition plate at intervals along the direction far away from the behavior test box, and the partition plate is detachably mounted in any one of the first clamping positions;
the side edge of the opening is provided with a second clamping position for fixing the partition plate; when the baffle joint was in the second screens, the baffle will dodge the case and keep apart with the action test box.
2. The apparatus for detecting the instinctive fear behavior of an animal according to claim 1, wherein: the visual stimulation device is arranged above or on the periphery of the behavior test box.
3. The apparatus for detecting the instinctive fear behavior of an animal according to claim 2, wherein: the visual stimulation device is a display screen.
4. The apparatus for detecting the instinctive fear behavior of an animal according to claim 1, wherein: the camera is disposed over the opening.
5. The apparatus for detecting instinctive fear behavior of an animal according to any one of claims 1 to 4, wherein: the animal instinct fear behavior detection device further comprises a support used for supporting the visual stimulation device and the camera, and the support is arranged outside the box body.
6. The apparatus for detecting instinctive fear behavior of an animal according to any one of claims 1 to 4, wherein: and an outer wall for shading and insulating sound is arranged at the periphery of the behavior test box.
7. The detection method of the instinct fear behavior of the animal is characterized by comprising the following steps: the method comprises the following steps:
placing an animal in a housing of an animal instinctive phobic behavior detecting device of any one of claims 1-6;
the visual stimulation device sends a stimulation signal to the animal, and the animal makes an instinct fear response after receiving the stimulation signal;
the camera records the behavioral response of the animal.
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| CN201910514494.8A CN110278884B (en) | 2019-06-14 | 2019-06-14 | Detection device and detection method for animal instinct fear behavior |
| PCT/CN2020/087753 WO2020248739A1 (en) | 2019-06-14 | 2020-04-29 | Apparatus and method for detecting instinctive fear behavior of animal |
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| CN201910514494.8A CN110278884B (en) | 2019-06-14 | 2019-06-14 | Detection device and detection method for animal instinct fear behavior |
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| CN110278884B (en) * | 2019-06-14 | 2021-09-03 | 中国科学院深圳先进技术研究院 | Detection device and detection method for animal instinct fear behavior |
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| WO2023108562A1 (en) * | 2021-12-16 | 2023-06-22 | 中国科学院深圳先进技术研究院 | Experimental apparatus for visual cue-type conditional fear experiment of experimental animal |
| WO2023108561A1 (en) * | 2021-12-16 | 2023-06-22 | 中国科学院深圳先进技术研究院 | Experimental apparatus |
| CN114451326A (en) * | 2022-02-10 | 2022-05-10 | 河南中医药大学 | Animal fear stress response detection system |
| CN115919254A (en) * | 2022-11-23 | 2023-04-07 | 中国科学院深圳先进技术研究院 | Method and system for integrating ability of vision and hearing of animal |
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