WO2020248739A1

WO2020248739A1 - Apparatus and method for detecting instinctive fear behavior of animal

Info

Publication number: WO2020248739A1
Application number: PCT/CN2020/087753
Authority: WO
Inventors: 刘清晴; 钟金玲; 杨星; 王立平
Original assignee: 中国科学院深圳先进技术研究院
Priority date: 2019-06-14
Filing date: 2020-04-29
Publication date: 2020-12-17
Also published as: CN110278884A; CN110278884B

Abstract

Provided are an apparatus and method for detecting an instinctive fear behavior of an animal. The apparatus for detecting an instinctive fear behavior of an animal comprises: a box body, wherein the box body comprises a behavior test box for testing an instinctive fear behavior of an animal and a hiding box in which the animal hides after the animal makes an instinctive fear response, and an opening for communicating the behavior test box and the hiding box is provided between the behavior test box and the hiding box; a visual stimulation apparatus for sending a stimulus signal to the animal so as to stimulate the animal to make the instinctive fear response; and a camera arranged on an upper portion of the box body and used for recording a behavior response of the animal. The apparatus and method for detecting an instinctive fear behavior of an animal provided in the present application can accurately obtain a movement path and the movement speed of an animal, thereby effectively improving the reliability and stability of experiment results.

Description

Animal instinctive fear behavior detection device and detection method

Technical field

The invention belongs to the technical field of animal behavior, and more specifically, relates to an animal instinctive fear behavior detection device and detection method.

Background technique

Fear, as one of the six basic emotional behaviors, is induced by the subject's perception of danger. The defensive behavior generated by fear is an important guarantee for animal survival and reproduction, and is the most important and conservative part of the evolution of species.

Disorders of fear regulation may lead to a series of related diseases, such as panic disorder and specific phobias. The research on the instinctive fear loop will help to find the mechanism of this kind of mental illness and develop new diagnosis and treatment strategies. Animal models used in loop research, especially mouse models, are widely used to study mental illness. For mice, avoiding aerial predators, such as eagles, is an important survivability. It may be mediated by specific neural circuits.

At present, devices used in the laboratory to study the instinctive fear behavior of mice usually place a cover in the box, which makes it difficult for the mouse to take pictures of its behavior after escaping into the cover. In the prior art, in order to capture the environment in the cover, a camera usually needs Shooting from the side resulted in serious picture distortion, and it was impossible to know the exact path of the mouse's movement, nor to analyze the speed of the mouse's movement.

technical problem

The purpose of the present invention is to provide an animal instinctive fear behavior detection device to solve the technical problem in the prior art that the movement path of the animal cannot be accurately obtained.

Technical solutions

In order to achieve the above objective, the technical solution adopted by the present invention is to provide an animal instinctive fear behavior detection device, including:

A box, the box comprising a behavior test box for animals to perform an instinctive fear behavior test and an avoiding box for animals to avoid after making an instinctive fear response, between the behavior test box and the avoiding box Is provided with an opening for connecting the behavior test box and the avoiding box;

Visual stimulation device, used to send stimulus signals to animals to stimulate the animals to make instinctive fear responses; and,

The camera is arranged above the box and is used to record the behavioral response of the animal.

Further, the cross-sectional shape of the side wall of the behavior test box is circular or elliptical.

Further, the visual stimulation device is arranged above or on the peripheral side of the behavior test box.

Further, the visual stimulation device is a display screen.

Further, the camera is arranged above the opening.

Further, the animal instinctive fear behavior detection device further includes a partition, and a plurality of clamping positions for fixing the partition are spaced in the avoiding box along a direction away from the behavior test box, and the partition is detachable Install in any first card slot.

Further, the side edge of the opening is provided with a second locking position for fixing the partition.

Further, the animal instinctive fear behavior detection device further includes a bracket for supporting the visual stimulation device and the camera, and the bracket is arranged outside the box.

Further, the outer periphery of the behavior test box is provided with an outer wall for shading and sound insulation.

The present invention also provides a method for detecting animal instinctive fear behavior, which includes the following steps:

Placing the animal in the box of the device for detecting animal instinctive fear behavior as described in any one of the above;

The visual stimulation device sends out a stimulation signal to the animal, and the animal makes an instinctive fear response after receiving the stimulation signal;

The camera records the animal's behavioral response.

Beneficial effect

The beneficial effects of the air-conditioned animal instinctive fear behavior detection device and detection method provided by the present invention are: Compared with the prior art, the animal instinctive fear behavior detection device of the present invention is provided with a behavior test box and an avoidance box, and a behavior test box and avoidance There is an opening between the boxes for connecting the behavior test box and the avoiding box, so that the camera can be set above the box, so that the camera can shoot from above, so that the shooting picture becomes smaller, and the animal's movement path can be accurately obtained And the movement speed, effectively improve the reliability and stability of the experimental results, and avoid the prior art that the camera can only shoot from the side due to the setting of the shield in the box body, which causes the shooting picture to be deformed and thus cannot obtain the exact movement path of the animal.

Description of the drawings

In order to explain the technical solutions in the embodiments of the present invention more clearly, the following will briefly introduce the drawings that need to be used in the description of the embodiments or the prior art. Obviously, the drawings in the following description are only of the present invention. For some embodiments, for those of ordinary skill in the art, other drawings can be obtained from these drawings without creative labor.

1 is a schematic diagram of a three-dimensional structure of an animal instinctive fear behavior detection device provided by an embodiment of the present invention;

2 is a schematic diagram of a three-dimensional structure of a box provided by an embodiment of the present invention;

3 is a schematic diagram of a three-dimensional structure of an animal instinctive fear behavior detection device provided by another embodiment of the present invention.

Among them, the reference signs in the figure:

10-box; 11-behavior test box; 12-evasion box; 13-opening; 20-camera; 30-partition; 40-bracket; 50-exterior wall.

Embodiments of the invention

In order to make the technical problems, technical solutions and beneficial effects to be solved by the present invention clearer, the following further describes the present invention in detail with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, but not to limit the present invention.

It should be noted that when an element is referred to as being "fixed to" or "disposed on" another element, it can be directly on the other element or indirectly on the other element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or indirectly connected to the other element.

It should be understood that the terms "length", "width", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top" , "Bottom", "Inner", "Outer" and other indications of the orientation or positional relationship are based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying the pointed device Or the element must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation of the present invention.

In addition, the terms "first" and "second" are only used for descriptive purposes, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of indicated technical features. Thus, the features defined with "first" and "second" may explicitly or implicitly include one or more of these features. In the description of the present invention, "plurality" means two or more than two, unless specifically defined otherwise.

Please refer to FIG. 1 and FIG. 2 together, the device for detecting animal instinctive fear behavior provided by the present invention will now be described. The present invention provides an animal instinctive fear behavior detection device, which includes a box 10, a visual stimulation device (not shown in the figure) and a camera 20. Visual stimulation devices are used to send stimulus signals to animals to stimulate the animals to make instinctive fear responses. Among them, mice are selected for the experiment in this embodiment. The camera 20 is arranged above the box 10 and is used to record the behavior response of the animal. The box body 10 includes a behavior test box 11 and an escape box 12, and the behavior test box 11 is used for animals to perform an instinctive fear behavior test. The avoiding box 12 is used for animals to avoid after making an instinctive fear response. An opening 13 is provided between the behavior test box 11 and the avoidance box 12 for connecting the behavior test box 11 and the avoidance box 12. When the animal is stimulated by the visual stimulation device, it will react instinctively and enter the avoidance box through the opening 13 Avoid within 12. The present invention separates the behavior test box 11 from the avoiding box 12, so that the camera 20 can shoot from above, thereby making the shooting picture clear, and avoiding the prior art that the camera 20 can only Shooting from the side causes the shooting picture to be deformed, which leads to the situation where the exact path of the animal cannot be obtained.

Compared with the prior art, the animal instinctive fear behavior detection device provided by the present invention is provided with a behavior test box 11 and a hiding box 12, and a behavior test box 11 and a hiding box are provided between the behavior test box 11 and the hiding box 12 The opening 13 of 12 allows the camera 20 to be arranged above the box 10, so that the camera 20 can shoot from above, so that the shooting picture becomes smaller, and the animal's movement path and speed can be accurately obtained, which effectively improves the experimental results The reliability and stability of this avoids the fact that the camera 20 can only shoot from the side due to the cover provided in the box 10 in the prior art, which causes the shooting picture to be deformed and thus cannot obtain the exact movement path of the animal.

Further, referring to FIGS. 1 and 2, as a specific embodiment of the animal instinctive fear behavior detection device provided by the present invention, the cross-sectional shape of the side wall of the behavior test box 11 is circular. Because traditional behavior testing devices usually use a rectangular parallelepiped or cube box with four corners, when the mouse faces the shadow of a simulated natural enemy, the corners may also be regarded as safe areas, thus introducing the output of instinctive fear behavior Uncertain factors lead to inaccurate test results. In the present invention, by setting the cross-sectional shape of the side wall of the behavior test box 11 to be circular, there is no corner problem, which improves the stability and reliability of the experiment, and solves the problems caused by the use of a square box in the prior art. There are corners between the side walls, which makes it easy for animals to regard the corners between the side walls as a safe area, which leads to inaccurate detection results. It should be noted that the cross-sectional shape of the side wall of the behavior test box 11 is not limited to this. For example, in other preferred implementations of the present invention, the cross-sectional shape of the side wall of the behavior test box 11 may also be elliptical. .

Further, as a specific implementation of the animal instinctive fear behavior detection device provided by the present invention, the visual stimulation device is arranged above the behavior test box 11, so that the response of the mouse to the visual hazard stimulus of the upper visual field can be detected. The stimulation signal sent by the visual stimulation device can be a black disc. It should be noted that the cross-sectional shape of the side wall of the behavior test box 11 is not limited to this. For example, in other preferred implementations of the present invention, the visual stimulation device may also be arranged on the peripheral side of the behavior test box 11. So as to detect the behavioral response of mice to visual stimuli from all around. Preferably, the visual stimulation device is a display screen. By using the display to show the black disc.

Further, referring to FIG. 1 and FIG. 2, as a specific implementation of the animal instinctive fear behavior detection device provided by the present invention, the camera 20 is disposed above the opening 13. By installing the camera 20 above the opening 13, it is convenient for the camera 20 to capture all of the behavior test box 11 and the avoiding box 12, and there is no dead spot for shooting. Preferably, the camera 20 is a wide-angle camera. In order to prevent the visual stimulation device from blocking the shooting of the camera 20, the height of the camera 20 should be lower than the height of the visual stimulation device.

Further, referring to Figures 1 and 2, as a specific implementation of the animal instinctive fear behavior detection device provided by the present invention, the animal instinctive fear behavior detection device further includes a partition 30, and the avoidance box 12 is far away from the behavior test box 11 A plurality of first detents (not shown in the figure) are arranged at intervals in the direction, where the escape box 12 is in a direction away from the behavior test box 11 in this embodiment refers to the length of the escape box 12, and the first detent can be used to fix the compartment. The plate 30 can be detachably installed in any of the first clamping positions, so that the escape box 12 can be divided into different lengths, and the length of the escape box 12 can be changed according to actual needs. Specifically, the first card position may be a recess provided on the side wall of the shelter, and only the partition board needs to be placed in the recess, and the operation is very simple and convenient.

Further, referring to FIG. 1 and FIG. 2, as a specific implementation of the animal instinctive fear behavior detection device provided by the present invention, the side edge of the opening 13 is provided with a second locking position (not shown), and the second locking position Used to fix the partition 30. When the partition 30 is clamped on the second card position, the partition 30 separates the escape box 12 from the behavior test box at this time. The mice can only move in the behavior test box 11 and cannot enter the escape box 12. It can be compared with the case where there is a shelter 12.

Further, as a specific implementation of the animal instinctive fear behavior detection device provided by the present invention, the animal instinctive fear behavior detection device further includes a bracket 40 for supporting the visual stimulation device and the camera 20, and the bracket 40 is arranged in the box 10 In addition, the visual stimulation device and the camera 20 can be fixed on the top of the box 10 through the setting of the bracket 40.

Further, referring to FIG. 3, as a specific embodiment of the animal instinctive fear behavior detection device provided by the present invention, an outer wall 50 is provided on the outer periphery of the behavior test box 11, and the outer wall 50 is used for shading and sound insulation, thereby making the experiment The conditions are stable.

Further, referring to FIG. 1, as a specific embodiment of the animal instinctive fear behavior detection device provided by the present invention, the height of the box 10 is 25cm-35cm, preferably 30cm, so as not only to effectively prevent mice from escaping, but also It can ensure that the mouse has a sufficiently wide upper field of view. The diameter of the behavior test box 11 is 45 cm-55 cm, preferably 50 cm. The avoiding box 12 has a rectangular parallelepiped structure. The length of the avoiding box 12 is 45 cm-55 cm and the width is 5 cm-15 cm. For example, in one of the embodiments of the present invention, the length of the avoiding box 12 is 50 cm and the width is 10 cm.

Further, as a specific implementation of the animal instinctive fear behavior detection device provided by the present invention, the box body 10 is made of acrylic sheet, which is lightweight, inexpensive, non-toxic, easy to form, and has good acid and alkali resistance. Etc. Of course, the material of the box body 10 is not limited to this. For example, in other preferred embodiments of the present invention, the box body 10 may also be a thin metal plate. Specifically, the color of the box 10 can be changed according to the fur color of the mouse under study. For example, when the fur color of the mouse is black, the color of the bottom plate of the box 10 can be white, for example, it can be a white matte bottom plate. When the mouse's coat color is white, the color of the bottom plate of the box body 10 can be black, so that the best shooting effect can be achieved. Preferably, when it is necessary to shoot and record the behavior of the mouse from the side at the same time, a side plate made of transparent acrylic can also be used, so that the camera can conveniently record the movement of the mouse from all around.

S10. Place the animal in the box 10 of the above-mentioned animal instinctive fear behavior detection device.

S20. The visual stimulation device sends out a stimulation signal to the animal, and the animal makes an instinctive fear response after receiving the stimulation signal.

S30. The camera 20 records the animal's behavioral response.

The present invention provides a behavior test box 11 and a dodging box 12, and an opening 13 for connecting the behavior test box 11 and the dodging box 12 is provided between the behavior test box 11 and the dodging box 12, so that the camera 20 can be arranged above the box body 10. , So that the camera 20 can be photographed from above, so that the deformation of the photographed picture is reduced, and then the movement path and movement speed of the animal can be accurately obtained, which effectively improves the reliability and stability of the experimental results, and avoids the prior art due to the box body The setting of a shielding object in 10 causes the camera 20 to only shoot from the side, which causes the shooting picture to be deformed, which makes it impossible to obtain the exact movement path of the animal.

The above descriptions are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modification, equivalent replacement and improvement made within the spirit and principle of the present invention shall be included in the protection of the present invention. Within range.

Claims

An animal instinctive fear behavior detection device is characterized in that it includes:

A box, the box comprising a behavior test box for animals to perform an instinctive fear behavior test and an avoiding box for animals to avoid after making an instinctive fear response, between the behavior test box and the avoiding box Is provided with an opening for connecting the behavior test box and the avoiding box;

Visual stimulation device, used to send stimulus signals to animals to stimulate the animals to make instinctive fear responses; and,

The camera is arranged above the box and is used to record the behavioral response of the animal.
4. The animal instinctive fear behavior detection device according to claim 1, wherein the cross-sectional shape of the side wall of the behavior test box is circular or elliptical.
The animal instinctive fear behavior detection device according to claim 1, wherein the visual stimulation device is arranged above or on the side of the behavior test box.
The device for detecting animal instinctive fear behavior according to claim 3, wherein the visual stimulation device is a display screen.
The device for detecting animal instinctive fear behavior according to claim 1, wherein the camera is arranged above the opening.
7. The animal instinctive fear behavior detection device according to any one of claims 1 to 5, wherein the animal instinctive fear behavior detection device further comprises a partition, and the avoidance box is spaced apart in a direction away from the behavior test box A plurality of clamping positions for fixing the partition are provided, and the partition may be detachably installed in any one of the first clamping positions.
7. The animal instinctive fear behavior detection device according to claim 6, wherein the side edge of the opening is provided with a second locking position for fixing the partition.
The animal instinctive fear behavior detection device according to any one of claims 1-6, wherein the animal instinct fear behavior detection device further comprises a bracket for supporting the visual stimulation device and the camera, and The bracket is arranged outside the box.
The air conditioning system according to any one of claims 1-6, wherein the outer periphery of the behavior test box is provided with an outer wall for shading and sound insulation.
The method for detecting instinctive fear behavior of animals is characterized in that it includes the following steps:

Placing the animal in the box of the device for detecting animal instinctive fear behavior according to any one of claims 1-9;

The visual stimulation device sends out a stimulation signal to the animal, and the animal makes an instinctive fear response after receiving the stimulation signal;

The camera records the animal's behavioral response.