CN110558294B - Simple and efficient two-stage harmonia axyridis breeding method - Google Patents
Simple and efficient two-stage harmonia axyridis breeding method Download PDFInfo
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- CN110558294B CN110558294B CN201910935247.5A CN201910935247A CN110558294B CN 110558294 B CN110558294 B CN 110558294B CN 201910935247 A CN201910935247 A CN 201910935247A CN 110558294 B CN110558294 B CN 110558294B
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- 241000199694 Harmonia axyridis Species 0.000 title claims abstract description 104
- 238000009395 breeding Methods 0.000 title claims abstract description 75
- 230000001488 breeding effect Effects 0.000 claims abstract description 51
- 235000002637 Nicotiana tabacum Nutrition 0.000 claims abstract description 45
- 241001124076 Aphididae Species 0.000 claims abstract description 43
- 241000208125 Nicotiana Species 0.000 claims abstract description 40
- 241000255749 Coccinellidae Species 0.000 claims abstract description 31
- 238000000034 method Methods 0.000 claims abstract description 20
- 241000196324 Embryophyta Species 0.000 claims abstract description 17
- 241000721621 Myzus persicae Species 0.000 claims abstract description 16
- 230000008569 process Effects 0.000 claims abstract description 15
- 230000001502 supplementing effect Effects 0.000 claims abstract description 6
- 238000005286 illumination Methods 0.000 claims description 15
- 239000011324 bead Substances 0.000 claims description 9
- 244000061176 Nicotiana tabacum Species 0.000 claims description 5
- 238000011081 inoculation Methods 0.000 claims description 2
- 238000002955 isolation Methods 0.000 claims description 2
- 230000017448 oviposition Effects 0.000 abstract description 9
- 241000238631 Hexapoda Species 0.000 abstract description 8
- 240000006677 Vicia faba Species 0.000 description 15
- 235000010749 Vicia faba Nutrition 0.000 description 14
- 235000002098 Vicia faba var. major Nutrition 0.000 description 14
- 235000013601 eggs Nutrition 0.000 description 10
- 238000005516 engineering process Methods 0.000 description 7
- 230000000694 effects Effects 0.000 description 4
- 241000607479 Yersinia pestis Species 0.000 description 3
- 230000006399 behavior Effects 0.000 description 3
- 230000000903 blocking effect Effects 0.000 description 3
- 230000007613 environmental effect Effects 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 230000013011 mating Effects 0.000 description 3
- 230000029264 phototaxis Effects 0.000 description 3
- 230000037396 body weight Effects 0.000 description 2
- 230000009194 climbing Effects 0.000 description 2
- 230000009193 crawling Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000000442 meristematic effect Effects 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 244000045232 Canavalia ensiformis Species 0.000 description 1
- 235000010617 Phaseolus lunatus Nutrition 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000007937 eating Effects 0.000 description 1
- 230000035611 feeding Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 238000004804 winding Methods 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M1/00—Stationary means for catching or killing insects
- A01M1/02—Stationary means for catching or killing insects with devices or substances, e.g. food, pheronones attracting the insects
- A01M1/04—Attracting insects by using illumination or colours
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- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Environmental Sciences (AREA)
- Zoology (AREA)
- Animal Behavior & Ethology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Engineering & Computer Science (AREA)
- Insects & Arthropods (AREA)
- Wood Science & Technology (AREA)
- Catching Or Destruction (AREA)
Abstract
The invention relates to the field of insect breeding, and particularly discloses a simple and efficient two-stage breeding method for harmonia axyridis, which comprises the following steps: s1: inoculating aphids to tobacco leaves, and then putting the tobacco inoculated with the aphids into a net room for feeding; s2: expanding propagation of the myzus persicae, and inoculating ladybug egg masses when the population density of the aphids on the tobacco is more than 20000 heads/plant; s3: breeding the larvae of the harmonia axyridis, and periodically supplementing aphids to the harmonia axyridis before pupating; s4: and (3) breeding the adult harmonia axyridis, putting a new batch of harmonia axyridis egg blocks after all the harmonia axyridis pupate, trapping the adult harmonia axyridis at night by utilizing a one-way light trapping bottle after all the harmonia axyridis pupate, and transferring and breeding the trapped adult harmonia axyridis. The invention solves the problems of high breeding cost, small individual size and low egg laying amount of the harmonia axyridis caused by frequent replacement of host plants in the existing breeding process of the harmonia axyridis.
Description
Technical Field
The invention relates to the field of insect breeding, in particular to a simple and efficient two-stage breeding method for harmonia axyridis.
Background
Biological control is one of the main technologies for green prevention and control of agricultural and forestry pests, and natural enemy insects are the basis of biological control, so that the importance of the natural enemy insects is self-evident, and mass breeding of the natural enemy insects is the core technology of biological control. The harmonia axyridis is one of main predatory natural enemies of crop pest aphids, adults and larvae of the harmonia axyridis feed on the aphids, and the quantity of the aphids caught in one life is more than 6000, so that artificial large-scale breeding of the harmonia axyridis is the basis for utilizing the harmonia axyridis to control the aphids.
The traditional harmonia axyridis breeding technology mainly adopts a three-level breeding technology system of broad beans-aphids-ladybug, the growing period of broad beans is short, plants are short, the plants grow excessively in the later period and are easy to fall down, the meristematic capacity of the broad beans is weak, the leaves are fast aged, fresh broad beans (7-10 days 1 times on average) need to be replaced regularly in the breeding process, labor and time are consumed in the replacement process, the breeding cost of the harmonia axyridis is high due to the fact that the linking time of breeding and replacement of the broad beans is calculated, the technical difficulty is large, and the popularization and application effects are poor. In addition, the host plant is replaced every 7-10 days, so that the eating, mating, oviposition and other behaviors of the ladybug are interfered, and compared with the naturally-grown harmonia axyridis, the harmonia axyridis bred by adopting the traditional technology is small in size, low in egg laying amount, weak in predation capacity and high in death rate.
Disclosure of Invention
The invention aims to provide a simple and efficient two-stage breeding method for harmonia axyridis, which solves the problems of high breeding cost, small size of harmonia axyridis individuals and low egg laying amount of the harmonia axyridis caused by frequent replacement of host plants in the existing breeding process of the harmonia axyridis.
In order to achieve the purpose, the technical scheme of the invention is as follows: a simple and efficient two-stage breeding method for harmonia axyridis comprises the following steps:
s1: inoculating aphids to tobacco leaves, and then putting the tobacco inoculated with the aphids into a net room for feeding;
s2: expanding propagation of the myzus persicae, and inoculating ladybug egg masses when the population density of the aphids on the tobacco is more than 20000 heads/plant;
s3: breeding the larvae of the harmonia axyridis, and periodically supplementing aphids to the harmonia axyridis before pupating;
s4: and (3) breeding the adult harmonia axyridis, putting a new batch of harmonia axyridis egg blocks after all the harmonia axyridis pupate, trapping the adult harmonia axyridis at night by utilizing a one-way light trapping bottle after all the harmonia axyridis pupate, and transferring and breeding the trapped adult harmonia axyridis.
The principle and the beneficial effects of the technical scheme are as follows: in the technical scheme, a common three-level breeding technical system of harmonia axyridis broad bean-aphid-ladybug in the prior art is changed, the host plants bred by the harmonia axyridis are creatively changed, and the broad beans are changed into tobacco plants. The host plant broad beans commonly used in the prior art is short in growing period, short and small in plant, overgrowing in later period and easy to fall down, the broad beans are weak in meristematic capacity, leaves are fast aging, multiple factors are interwoven together to cause that fresh broad bean seedlings need to be replaced regularly (generally 1 time for replacement in 7-10 days) to meet the requirement of aphid feeding, broad beans seedlings of 4-5 times need to be replaced when a generation of ladybug is fed, time and labor are wasted, the production cost is increased, and the host plant replacement once in every 7 days also has great interference on behaviors of feeding, mating, oviposition and the like of the ladybug.
According to the technical scheme, after the host plant is replaced by the tobacco plant, the host plant does not need to be replaced in the harmonia axyridis larva-imago stage, and only a proper amount of myzus persicae needs to be supplemented according to the number of myzus persicae, so that the seedling replacement workload is reduced; and because the food is sufficient, the artificial interference is less, the adult ladybug is larger than other methods for feeding, the egg laying amount is improved, the predation amount of aphids is also improved, and the pest control capability of the harmonia axyridis is enhanced. In addition, in the growth process of the harmonia axyridis, the life habits of larvae and adults are different, but the traditional breeding technology of the harmonia axyridis ignores the difference; the sectional feeding mode of the technical scheme is carried out on the larvae and the imagoes of the harmonia axyridis, so that the feeding environment can be better adjusted to adapt to the life habits of the corresponding insect states, and the collection of the egg masses of the harmonia axyridis at the imagoes stage is facilitated.
In addition, the collection of the ladybug adults in the traditional harmonia axyridis breeding technology mainly depends on manual collection, the efficiency is low, the technical scheme utilizes the phototaxis of the ladybug adults and has the characteristic of certain flight capability, and adopts the suspended one-way illumination trapping bottle to trap the ladybug adults, so that the targeted automatic collection process of the ladybug adults is realized, the efficiency is high, and the accuracy is high.
Further, in S4, the one-way light trapping bottle is arranged in an inverted mode, the bottle opening of the one-way light trapping bottle is concave funnel-shaped, and a lamp strip is arranged in the one-way light trapping bottle.
Among this technical scheme, through set up the lamp area in the one-way light lures a set bottle, can make the ladybug adult be lured under the driving of phototaxis and collected in the one-way light lures a set bottle, because the bottleneck that the one-way light lured a set bottle sets up to the hourglass hopper-shaped of indent formula for the inboard illumination intensity of bottleneck is stronger than the illumination intensity in the bottleneck outside, can make the ladybug adult climb automatically in the one-way light lures a set bottle, avoids climbing out moreover, realizes effectual luring a set.
Further, in S4, the collected adults are transferred to a small breeding cage, and tubular sponge paper is set in the small breeding cage.
Among this technical scheme, through transferring the ladybug adult that lures the collection to little breed the cage in, can conveniently follow the adjustment of ladybug adult growth environment, do benefit to its growth and lay eggs, through set up the spongy paper of reel form in little breed cage, the collection of convenient ladybug egg mass.
Further, aphids are supplemented regularly in the breeding process of the ladybug larvae and the imagoes, and the tobacco is irrigated and fertilized.
In the technical scheme, the adult insect breeding process can ensure sufficient quantity of aphids by regularly supplementing aphids and regularly irrigating and fertilizing tobacco, thereby ensuring sufficient food sources of ladybug.
Further, in S1, inoculating aphids when the tobacco plants grow to the height of 30-40 cm.
In the technical scheme, when the tobacco grows to the plant height of 30-40 cm and then the aphids are inoculated, the aphids can be guaranteed to have sufficient tobacco leaves for feeding, so that the tobacco is prevented from dying after the aphids are inoculated, and the two-way survival rate of the aphids and the tobacco is guaranteed.
Further, in S2, the propagation conditions of myzus persicae are: the ambient temperature is 25-30 ℃, the relative humidity is 75%, the illumination L is 8:16, and the illumination intensity is more than or equal to 140 mu mol.m-2·s-1。
In the technical scheme, the temperature, the humidity and the illumination condition are suitable environmental conditions for aphid growth, and the myzus persicae can be propagated under the conditions, so that the effect of propagation of the myzus persicae can be ensured.
Further, in S4, the sponge paper was set in a cylindrical shape having a diameter of 1cm and a length of 15 cm.
In the technical scheme, the sponge paper is set into a cylinder shape with the diameter of 1cm and the length of 15cm, so that the environment of the tobacco leaf surface winding drum under natural conditions can be simulated, and the egg laying of the ladybug adult is accelerated.
Further, the method also comprises the tobacco seedling raising and tobacco cultivation processes before the tobacco aphid inoculation.
In the technical scheme, the tobacco is monitored in the whole flow from the seedling culture to the later cultivation process, so that the quality of tobacco plants can be ensured.
Further, the sponge paper is green or pale green.
In the technical scheme, the sponge paper is set to be green or light green, so that the color of the tobacco leaves under natural conditions can be further simulated, and the ladybug imagoes can be quickly adapted to the environment of the small breeding cage.
Further, lamp beads are fixed at the bottom of the one-way light trapping bottle, and the lamp strip and the lamp beads are yellow.
In the technical scheme, the lamp beads are arranged at the bottom of the one-way trapping bottle, after the one-way trapping bottle is hung, the brightness inside the trapping bottle can be further ensured, yellow is a sensitive light source color of the adult ladybug, the lamp strip and the lamp beads are both set to be yellow, and light emitted by the lamp strip and the lamp beads is also yellow, so that the trapping effect on the adult ladybug is improved.
Drawings
Fig. 1 is a schematic structural diagram of an attraction device according to an embodiment of the present invention.
Detailed Description
The following is further detailed by way of specific embodiments:
reference numerals in the drawings of the specification include: one-way light lures a bottle 1, link 2, lures a mouthful 3, lamp area 4, lamp pearl 5, shifts pipe 6, receiving flask 7, barrier paper 8, stop curtain 9.
The harmonia axyridis breeding test is divided into two treatment groups, namely a two-section breeding treatment group (adopting the breeding method of the invention) and a traditional breeding treatment group (adopting the traditional lima bean breeding method), each treatment group is provided with 3 repetitions, each repetition is 8 pairs of harmonia axyridis, the male-female ratio is 1: 1. the feeding environmental conditions for both treatment groups were: the ambient temperature is 28 ℃, the relative humidity is 75%, and the illumination L: D is 8: 16.
As shown in FIG. 1, the method for raising harmonia axyridis in the two-stage raising treatment group comprises the following steps:
s1: tobacco seedling raising is carried out according to conventional tobacco seedling raising measures, and the process is not repeated herein;
s2: tobacco cultivation, namely transplanting tobacco seedlings when the height of the seedlings is 8cm after the tobacco seedlings are in a seedling stage, taking flowerpots with the diameter of 35cm and the depth of 40cm, transplanting 1 tobacco seedling in each pot, and filling nutrient soil in each pot;
s3: inoculating aphids to the tobacco, and inoculating the aphids when the transplanted tobacco grows to the height of 40cm, wherein the process of inoculating the aphids is as follows: the tobacco leaves with the myzus persicae are placed on the tobacco to be connected with the aphids, the aphids are enabled to be freely transferred and diffused, the tobacco for raising the myzus persicae is placed in the net room, the aphids are prevented from flying out or external insects from entering the net room, and the trapping device is hung in the net room, the trapping device in the embodiment comprises a one-way light trapping bottle 1, the one-way light trapping bottle 1 comprises a bottle body which is hung upside down, the bottom of the bottle body is arranged in a concave manner and is integrally funnel-shaped, a funnel-shaped small-diameter end formed at the bottom of the bottle body is provided with a trapping port 3, the caliber of the trapping port 3 is 5.3mm (generally, the length of an adult of the heterochromatic ladybug is 5.4-8.0mm, and the body width is 3.8-5.2mm, so that the situation that the bore of the trapping port 3 is only slightly larger than the body width of the heterochromatic ladybug in the process that the adult ladybug crawls inwards along the trapping port 3 when being trapped, the adult ladybug is prevented from turning around the trapping port, and only can continuously crawl inwards, only the harmonia axyridis can enter and not exit), a hanging ring 2 is bonded at the bottom of the upper end of the bottle body and used for realizing the upside-down hanging of the one-way light trapping bottle 1; a plurality of groups of yellow lamp belts 4 are fixedly connected in the bottle body, the brightness of the lamp belts 4 is sequentially increased from bottom to top, and the bottle mouth is positioned between two adjacent lamp belts 4, so that when the harmonia axyridis climbs to the bottle mouth, the brightness above the trapping port 3 in the graph 1 is greater than the brightness at the bottle mouth, and the driving force for the harmonia axyridis to continuously move to the interior of the bottle body can be given; three yellow lamp beads 5 are fixedly connected to the top in the bottle body; the top (yellow lamp area 4 brightest department) intercommunication of bottle has the transfer pipe 6, it has yellow lamp area 4 also to bond in the pipe 6 to transfer, the lamp area 4 in the pipe 6 shifts is from the right side luminance crescent gradually, the diameter that shifts the pipe 6 is 5.3mm, it is provided with the ring channel of embedding lamp area 4 to shift 6 inner walls of pipe, and behind the lamp area 4 embedding ring channel, its inboard is located same water flat line with the inner wall that shifts the pipe 6, and then guarantee that the heterochromatic ladybug when passing through from shifting the pipe 6, crawl at the horizontal plane always. The left end of the transfer tube 6 is communicated with a collecting bottle 7, the upper end of the collecting bottle 7 is also fixedly connected with a hanging ring 2, isolation paper 8 is adhered to the outer part of the collecting bottle 7, a plurality of blocking curtains 9 capable of blocking the tube orifice of the transfer tube 6 are adhered to the inner top wall of the collecting bottle 7, and the blocking curtains 9 are green strip-shaped soft paper;
s4: expanding propagation of the myzus persicae, keeping the environmental temperature at 28 ℃, the relative humidity at 75%, the illumination L: D is 8:16, the illumination intensity is 140 mu mol.m-2. s-1, and when the population density of the aphids exceeds 20000 heads per plant, putting the egg mass of the harmonia axyridis into the breeding pond for breeding the harmonia axyridis;
s5: breeding the harmonia axyridis larvae, namely putting the eggs or larvae to be hatched of the harmonia axyridis into the tobacco breeding with aphids in the cover net, ensuring that the number of the aphids is at least 400 times of the number of the harmonia axyridis, freely feeding the harmonia axyridis larvae on the tobacco until the harmonia axyridis is pupated, and periodically inoculating and supplementing the aphids according to the number of the aphids on the tobacco during breeding, so as to ensure that the harmonia axyridis food is sufficient; reasonably irrigating and fertilizing according to the soil moisture content of the tobacco to ensure the growth of the tobacco; during the period, if the ladybug larvae need to be sold, the ladybug larvae can be manually collected and packaged for sale;
s6: breeding the adult harmonia axyridis, putting another batch of new harmonia axyridis egg blocks after all the harmonia axyridis pupate, and starting breeding the new harmonia axyridis; after all the harmonia axyridis pupae are eclosized, utilizing the one-way illumination trapping bottle to trap harmonia axyridis adults at night, and opening the one-way illumination trapping bottle 1 and the lamp strip 4 and the lamp bead 5 in the transfer pipe 6, wherein the harmonia axyridis adults fly towards the one-way illumination trapping bottle 1 because of phototaxis and flight capability, and when the harmonia axyridis adults fall to the bottleneck, the brightness above the bottleneck is brighter than the bottleneck, so that the harmonia axyridis adults have the power of continuously crawling towards the interior of the bottle body; after the harmonia axyridis climbs into the bottle body, because the brightness of the lamp strip 4 in the bottle body is sequentially enhanced from bottom to top, the harmonia axyridis climbs towards the top end of the bottle body and is transferred towards the inside of the collecting bottle 7 along the transfer tube 6, because the brightness of the lamp strip 4 in the transfer tube 6 is gradually enhanced from right to left, the harmonia axyridis has the driving force of crawling towards the collecting bottle 7, and because the size of the transfer tube 6 is set, the harmonia axyridis can only crawl in one direction but can not return; after the harmonia axyridis is transferred into the collecting bottle 7, the baffle curtain 9 in the collecting bottle 7 can block the left end opening of the transfer pipe 6 to block the path inlet moving to the unidirectional light trapping bottle 1, so that the harmonia axyridis is prevented from climbing back to the unidirectional light trapping bottle 1; releasing the lured harmonia axyridis adults into corresponding small breeding cages for breeding in the next day, and placing light green sponge paper which is in a cylindrical shape (the diameter of the cylinder is 1cm, the length of the cylinder is 15cm) in the small breeding cages for the eggs laying of the harmonia axyridis; during the period, enough myzus persicae is supplemented in due time according to the feeding condition of the ladybug;
s7: during the breeding period of the harmonia axyridis larvae-imagoes, if production needs, artificial collection of the larvae and the unidirectional illumination trapping bottles 1 can be adopted to trap the imagoes, and then the imagoes are packaged for delivery.
The traditional broad bean breeding method is that after the aphids are inoculated on the broad bean seedlings, the broad bean seedlings inoculated with the aphids are placed in a net room and inoculated with the egg masses of the harmonia axyridis for breeding, and the broad bean seedlings are replaced every 7 days.
After breeding a first generation of harmonia axyridis, the body length, body weight and single-female egg laying amount of harmonia axyridis bred in the two breeding treatment groups and the traditional breeding treatment group were measured, respectively, and the results are shown in table 1 (mean ± standard deviation).
TABLE 1 comparison of two-stage breeding method for harmonia axyridis and traditional breeding method
As can be seen from Table 1, the harmonia axyridis raised by the two-stage raising method is higher than the harmonia axyridis raised by the conventional broad bean raising method in terms of body length and body weight. In the aspect of egg laying amount, the harmonia axyridis bred by the two-section breeding method is obviously higher than the harmonia axyridis bred by the traditional broad bean breeding method. The two-stage breeding method does not need to replace host plants in the larvae-imago stage of the harmonia axyridis, only needs to supplement a proper amount of myzus persicae according to the number of the myzus persicae, has little artificial interference and does not influence the tendency, mating and other behaviors of the harmonia axyridis. Therefore, the conclusion can be drawn that the harmonia axyridis bred by the two-section breeding method has better quality than the conventional breeding method, and meanwhile, the host plant does not need to be replaced in the larva-adult stage of the harmonia axyridis, so that the workload is greatly reduced.
The foregoing is merely an example of the present invention and common general knowledge in the art of designing and/or characterizing particular aspects and/or features is not described in any greater detail herein. It should be noted that, for those skilled in the art, without departing from the technical solution of the present invention, several variations and modifications can be made, which should also be regarded as the protection scope of the present invention, and these will not affect the effect of the implementation of the present invention and the practicability of the patent. The scope of the claims of the present application shall be determined by the contents of the claims, and the description of the embodiments and the like in the specification shall be used to explain the contents of the claims.
Claims (8)
1. A simple and efficient two-stage breeding method for harmonia axyridis is characterized by comprising the following steps:
s1: inoculating aphids to tobacco leaves, and then putting the tobacco inoculated with the aphids into a net room for feeding;
s2: expanding propagation of the myzus persicae, and inoculating ladybug egg masses when the population density of the aphids on the tobacco is more than 20000 heads/plant;
s3: breeding the larvae of the harmonia axyridis, and periodically supplementing aphids to the harmonia axyridis before pupating;
s4: breeding the adult harmonia axyridis, putting a new batch of harmonia axyridis egg blocks after all the harmonia axyridis pupate, trapping the adult harmonia axyridis at night by utilizing a one-way light trapping bottle after all the harmonia axyridis pupate, and transferring and breeding the trapped adult harmonia axyridis; the unidirectional light trapping bottle comprises a bottle body which is reversely hung, the bottom of the bottle body is concave, the whole bottle body is funnel-shaped, a trapping port is formed in a funnel-shaped small-diameter end formed at the bottom of the bottle body, the caliber of the trapping port is 5.3mm, a plurality of groups of yellow lamp strips are fixedly connected in the bottle body, the brightness of the lamp strips is sequentially increased from bottom to top, and the bottle mouth is positioned between two adjacent lamp strips; the brightness above the trapping port is greater than that at the bottle mouth, the top of the bottle body (the brightest part of the yellow lamp strip) is communicated with a transfer tube, the yellow lamp strip is also bonded in the transfer tube, the brightness of the lamp strip in the transfer tube is gradually increased from right to left, the diameter of the transfer tube is 5.3mm, an annular groove embedded into the lamp strip is formed in the inner wall of the transfer tube, and after the lamp strip is embedded into the annular groove, the inner side of the annular groove and the inner wall of the transfer tube are positioned on the same horizontal line; the left end of the transfer pipe is communicated with a collecting bottle, the outside of the collecting bottle is bonded with isolation paper, and the top wall of the inside of the collecting bottle is bonded with a plurality of retaining curtains capable of shielding the pipe orifice of the transfer pipe; transferring the trapped imagoes into a small breeding cage, and arranging tubular sponge paper in the small breeding cage.
2. The simple and efficient two-stage breeding method of harmonia axyridis as claimed in claim 1, wherein: regularly supplementing aphids and irrigating and fertilizing tobacco in the breeding process of ladybug larvae and adults.
3. The simple and efficient two-stage breeding method of harmonia axyridis as claimed in claim 2, wherein: and S1, inoculating aphids when the tobacco plants grow to the height of 30-40 cm.
4. The simple and efficient two-stage breeding method of harmonia axyridis as claimed in claim 3, wherein: in S2, the propagation conditions of the myzus persicae are as follows: the ambient temperature is 25-30 ℃, the relative humidity is 75%, the illumination L is 8:16, and the illumination intensity is more than or equal to 140 mu mol.m-2·s-1。
5. The simple and efficient two-stage breeding method of harmonia axyridis as claimed in claim 4, wherein: in S4, the sponge paper was set in a cylindrical shape having a diameter of 1cm and a length of 15 cm.
6. The simple and efficient two-stage breeding method of harmonia axyridis as claimed in claim 5, wherein: before the aphid inoculation of the tobacco, the tobacco seedling raising and tobacco cultivation processes are also included.
7. The simple and efficient two-stage breeding method of harmonia axyridis as claimed in claim 6, wherein: the sponge paper is green or light green.
8. The simple and efficient two-stage breeding method of harmonia axyridis as claimed in claim 7, wherein: the lamp strip is characterized in that a lamp bead is further fixed at the bottom of the unidirectional light trapping bottle, and the lamp strip and the lamp bead are yellow.
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| CN208191925U (en) * | 2018-05-23 | 2018-12-07 | 重庆市农业科学院 | A kind of parasitic wasp artificial feeding collection device |
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