CN111480621A - Method for collecting and feeding cadaveric beetles in laboratory - Google Patents
Method for collecting and feeding cadaveric beetles in laboratory Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M1/00—Stationary means for catching or killing insects
- A01M1/02—Stationary means for catching or killing insects with devices or substances, e.g. food, pheronones attracting the insects
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M1/00—Stationary means for catching or killing insects
- A01M1/10—Catching insects by using Traps
- A01M1/103—Catching insects by using Traps for crawling insects
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Abstract
The invention provides a collection and laboratory feeding method of autophagous beetles, which comprises the following steps: step 1, collecting adult or larva of cadaveric beetles in the field; step 2, preparing a culture medium and a feed for a cadaveric beetle laboratory; step 3, feeding cadaveric beetles in a laboratory; step 4, hatching, molting, pupating, eclosion and passage; step 5, collecting development data of each life stage of the autophagous beetles; and 6, measuring the body length of each larva stage of the autophagous beetle. The method has the advantages of simple and convenient operation, strong practicability, wide and easily obtained raw materials, low cost and convenient popularization and use, and comprises a series of operations such as beetle trapping, feeding, passage, data collection and the like, so that the method can meet the requirements of laboratories of colleges and universities or scientific research institutes on the research on the cadaveric beetles and also provides practical requirements for criminal case investigation related to the inference of death time of the cadaveric beetles.
Description
Technical Field
The invention relates to medical and insect science, in particular to a method for collecting cadaveric beetles and feeding the same in a laboratory.
Background
Time of death (PMI) inference is one of the key links for criminal case investigation, but PMI inference on putrefactive carcasses has always been a difficult problem in forensic practice. Forensic entomology is a novel cross-type discipline that uses the growth and development laws of insects on spoiled bodies to make PMI inferences, which can effectively infer PMI of spoiled bodies.
In the field of law medicine entomology, the coleopteran cadaveric beetle has important significance for deducing putrefactive late death time, and for scientific research and criminal case investigation, people need to collect in the field, carry out subculture and observation on various cadaveric beetles in a laboratory, and record the growth and development rules, body length change and other data of the beetles in various life stages.
However, a standard collection and laboratory subculture method of the cadaveric beetles does not exist so far, which seriously limits the research and application of the cadaveric beetles in the field of forensic entomology, and therefore, the collection and laboratory subculture method of the cadaveric beetles is very important for forensic entomology and scientific research work.
Disclosure of Invention
The invention provides a collection and laboratory feeding method of cadaveric beetles, and aims to provide a standard collection and laboratory passaging feeding method of the cadaveric beetles so as to meet the research and application of the cadaveric beetles in the field of legal medical sciences and insecticity,
in order to achieve the above object, an embodiment of the present invention provides a method for collecting and laboratory-feeding cadaveric beetles, comprising the steps of:
step 1, collecting adult or larva of cadaveric beetles in the field:
the rotten meat is used as a bait, and the simple and easy cadaveric beetle trapping device is used for trapping the adults or larvae of the cadaveric beetles;
fine sand, humus, sawdust and weeds are used as laboratory culture substrates to manufacture culture boxes, and fly larvae or pupae and putrefactive later-stage corpses are used as feeds to manufacture feed boxes;
step 3, feeding cadaveric beetles in a laboratory:
putting the culture box and the feed box manufactured in the step 2 into a shading or low-light environment with the temperature of 25 ℃ and the humidity of 75% for feeding the autophagous beetles;
and 6, measuring the body length of the larvae of the autophagous beetles.
Preferably, in the step 1, the specific method for collecting the cadaveric beetle adults or larvae in the field is as follows: the simple and easy trapping device for the cadaveric beetles is placed on a wild grassland or a forest where the cadaveric beetles usually appear, rotten meat is placed in the simple and easy trapping device and serves as food for trapping the beetles, after 5-7 days, the cadaveric beetles collected in the device are observed and collected, and then the cadaveric beetles are brought back to a laboratory for further breeding.
Preferably, the cadaveric beetle trapping device is placed in a place that is protected from direct sunlight, while avoiding rainwater accumulation.
Preferably, simple and easy trapping device of addict beetle constitute by iron cage, black plastic bag and bait basin, iron cage includes frame, six wire netting, and four angles in bottom surface are equipped with outstanding nail, can advance earth with the nail when placing to prevent that the cage from being blown down or the beast from pounding. The frame adopts the heavy bar in order to increase cage weight, the wire netting clearance can hold the beetle and get into and can prevent that the food of cage the inside from being eaten by the animal that the bigger beetle of volume, iron cage top iron wire netting can be opened, black plastic bag is upwards uncovered and the upper end is fixed in on four angles at cage top, the bait basin is arranged in inside the black plastic bag, bait pelvic floor portion is spread there is fine sand.
Preferably, in the step 2, the specific method for preparing the culture medium and the feed for the cadaveric beetle laboratory is as follows:
step 21, preparation of culture medium: laying a layer of sterilized fine sand in a culture box, placing a layer of humus or leaf rotting soil on the fine sand, then placing a layer of wet fermented wood chips, placing a layer of weeds on the wood chips to form a culture medium or a hiding place of the cadaveric beetle, and regularly spraying water by using a kettle to keep a wet living environment of the beetle;
Preferably, in the step 3, the laboratory breeding method of the cadaveric beetles comprises the following specific steps: firstly, the collected cadaveric beetles in the step 1 are placed into a simple cadaveric beetle laboratory feeding device with the culture box and the feed box arranged inside, then the simple cadaveric beetle laboratory feeding device is placed into an insect artificial climate box with the temperature of 25 ℃, the humidity of 75%, shading or weak light for feeding, the feed prepared in the step 2 is observed and fed regularly, water is sprayed regularly to meet the growth requirement of the cadaveric beetles, and the culture medium of the cadaveric beetle laboratory is replaced every 3-5 intervals to prevent the growth of mites and fungi.
Preferably, in step 3, the simple and easy feeding device in autophagous beetle laboratory include detachable cage, six faces of cage are connected with the nylon wire net, and one of them side is equipped with the zip fastener, put the culture box and the feed box of step 2 preparation in the cage, the feed box is located the one corner in the culture box, the feed box is culture dish or plastics dish.
Preferably, in the step 4, the specific methods of hatching, molting, pupating, eclosion and passage are as follows: when the male and female adults reach sexual maturity, namely the male and female adults are observed to have mating behaviors, putting an egg collector containing rotten meat into the culture box, taking out the born cadaveric beetle eggs and placing the cadaveric beetle eggs into a plastic culture dish of which the bottom comprises wet white paper towels within 4 hours after the eggs are observed for the first time, then placing the plastic culture dish containing the cadaveric beetle eggs into the bottom of an insect incubator covered by wet paper towels, and finally placing the insect incubator into an insect student artificial climate box with the constant temperature of 25 ℃, the humidity of 75% and shading or weak light for incubation; and after the eggs are hatched into larvae, immediately transferring the larvae from the plastic culture dish into another simple breeding device of a cadaveric beetle laboratory in the same climatic chamber for breeding, and enabling the larvae to undergo molting and develop to the next stage. After entering the larva terminal period, the larva does not eat any more, the body surface color gradually turns yellow, and then the larva penetrates into the culture medium to prepare pupation; after the larvae are pupated, screening the cadaveric beetle pupae out of the culture medium by using a screen, sterilizing the screened culture medium and then reusing the screened culture medium, placing the screened beetle pupae into an open plastic box, then placing the box at the bottom of an insect box covered by a wet paper towel, and finally placing the insect box filled with the cadaveric beetle pupae into an insect-learning artificial climate box with the constant temperature of 25 ℃ and the humidity of 75 percent and light shading or weak light for continuous development; and (3) after the pupae of the autophagous beetle emerge into adults, transferring the adults into another simple breeding device in an autophagous beetle laboratory for breeding, adding sufficient feed manufactured in the step 2, inducing the adults to lay eggs after the adults are sexually mature, completing passage for one time, and then repeatedly breeding by passage.
Preferably, in the step 5, the specific method for collecting development data of each life stage of the cadaveric beetles comprises the following steps: the time that the eggs of the same batch of cadaveric beetles are put into an insect incubator is the starting time of the egg phase, when more than 50% of the eggs of the cadaveric beetles are observed to be incubated into larvae, the ending time of the egg phase of the beetles is recorded, and the starting time of the larva phase is also recorded, when more than 50% of the larvae are observed to be pupated, the starting time of the pupal phase is recorded, when more than 50% of pupae of the cadaveric beetles are eclosion into adults, the ending time of the pupal phase is recorded, and the ending time of the incubation period is also marked.
Preferably, in the step 6, the specific method for measuring the body length of each larva stage of the cadaveric beetle is as follows: randomly selecting 5-10 larvae every 24 hours from the beginning time of a larva stage, putting the larvae into a thin and long transparent glass tube, measuring the body length of the larvae by using an original point type digital display caliper, measuring the average value of the body length of the larvae for three times to serve as the body length value of the larvae at the moment, and putting the measured larvae back into a breeding device for continuous breeding; when more than 50% of the larvae have completed pupation, the larval stage is marked to the end, at which time sampling and measurement of the larvae is stopped.
The scheme of the invention has the following beneficial effects: the method has the advantages of simple and convenient operation, strong practicability, wide and easily obtained raw materials, low cost and convenient popularization and use, and comprises a series of operations such as beetle trapping, feeding, passage, data collection and the like, so that the method can meet the requirements of laboratories of colleges and universities or scientific research institutes on the research on the cadaveric beetles and also provides practical requirements for criminal case investigation related to the inference of death time of the cadaveric beetles.
Drawings
FIG. 1 is a schematic diagram of a simple cadaveric beetle trapping device in an embodiment of the present invention.
FIG. 2 is a schematic view of a simple feeding device for a cadaveric beetle laboratory according to an embodiment of the present invention.
FIG. 3 shows the body length measurements of the stages of the beetles cadaveric in the examples of the present invention.
[ description of reference ]
1-iron cage; 11-a frame; 12-wire netting; 13-nailing; 2-black plastic bag; 3-a bait basin; 4-cage; 41-nylon mesh; 42-a zipper; 5-culture box; 6-a feed box.
Detailed Description
In order to make the technical problems, technical solutions and advantages of the present invention more apparent, the following detailed description is given with reference to the accompanying drawings and specific embodiments.
The field collection and laboratory rearing of this example was performed at the Zhongnan university forensic insect field base and laboratory. In a sunny day in 10 months, the temperature is 20-30 ℃, the simple cadaveric beetle trapping device is placed on a grassland where the beetles often appear in the field base of medical and insect science of southern China, and the place where the cadaveric beetle trapping device is placed avoids the direct sunlight and simultaneously avoids rainwater accumulation. One rabbit carcass was placed in the device as food for trapping beetles, and after 7 days, the cadaveric adults and larvae of beetles were observed and collected in the device, and then taken back to the laboratory.
A simple and easy cadaveric beetle trapping device is shown in figure 1: the square iron cage 1 of a 1m size, the connection frame 11 of cage 1 adopts heavier reinforcing bar to increase cage weight, and four angles have outstanding nail 13 below the iron cage simultaneously, can advance earth with the nail when placing to prevent to be blown down or the beast pounces on. Six faces of cage are wire netting 12, and the wire netting interval size is that animals such as dog cat mouse are difficult to stretch into to eat the food inside, but can go into to the beetle that the volume is less. The upper side of the iron cage is designed to be openable. Inside being an open black plastic bag 2 of iron cage, the ascending uncovered is tied up and is prevented to drop on four angles above the iron cage, and open plastic bag is inside places a bottom and has spread the plastic basin of fine sand as bait basin 3, and bait basin 3 is used for placing baits such as rabbit corpse.
The cadaveric beetles attracted by the cadaveric bodies in the bait basin 3 can climb to the opening of the black plastic bag 2 along the iron cage and then fall into the bait basin 3 inside to eat the cadaveric bodies, because the inner wall of the black plastic bag 2 is smooth and black in shading, fine sand and other hiding parts in the inner part of the bait basin 3 are arranged, the generally fallen cadaveric beetles are difficult to separate, after 5 to 7 days, the black plastic bag 2 and the bait basin 3 inside are brought back to a laboratory, and the fine sand with the embedded beetles in the bait basin 3 is screened by a screen mesh, so that adults or larvae of the cadaveric beetles can be obtained. This device. The efficiency of collecting the autophagous beetles in the field is greatly improved, and the device avoids the trouble of collecting and preventing the beetles from escaping every day and saves time.
The simple breeding device for the addicting beetle laboratory is shown in figure 2: the detachable cage 4 of square of a 1m size, six faces of cage 4 are connected with nylon wire 41, and one of them side can be opened open with zip fastener 42, and the culture box 5 of making is put to 4 insides of cage, then regard as feed box 6 with a great culture dish or plastics dish etc. feed box 6 places the one corner in culture box 5, and the corpse that holds the rabbit inside feed box 6 is used for the growth and development needs of the cephalosporium leucotrichum autophagous beetle. The nylon net 41 mainly functions to prevent adults or larvae from crawling out.
Preparing a culture medium and a feed for cadaveric beetles in a laboratory: a rectangular plastic box is adopted as a culture box 5, a layer of sterilized fine sand is firstly paved in the culture box 5, a layer of humus or leaf rotting soil is placed on the fine sand, a layer of wet fermented wood chips is placed on the wood chips, a layer of weeds is placed on the wood chips to form a culture medium of the autophilous beetles or a hiding place, and water is regularly sprayed by a kettle to keep a wet living environment of the beetles; the laboratory feed for the cadaveric beetles is mainly divided into 2 types, wherein the first type takes fly larvae or pupae as feed and is mainly used for feeding the cadaveric beetles in the families of buried beetles or cryptopteridae; the second kind is to use the putrefactive later stage corpse as the feed, including the corpse of rat or rabbit, mainly used for raising the pissodidae or Guogongjia corpse sex beetle, the corpse can be air-dried wholly and put into the feed box 6, or air-dried and put into the feed box 6 after smashing with the mixer. Since the beetles collected in this example were adults and larvae of the bark beetle, the carcass of the rabbit was used as feed.
Screening adult and larva of the cadaveric beetle in a collecting device out of sand to obtain about 30 adult and larva of the cadaveric beetle, identifying morphologic and molecular species of the adult and the larva of the cadaveric beetle to confirm that 11 of the cadaveric beetles of the albopic beetle are available, wherein 8 adults and 3 larvae of the adult and the larva of the albopic beetle are placed into a simple feeding device of the cadaveric beetle laboratory, then placing the simple feeding device of the cadaveric beetle laboratory into a shading artificial climate box for feeding insects, observing the corpses once every 24 hours and feeding the corpses of rabbits in the feeding box to meet the growth requirement of the cadaveric beetle of the albopic beetle, and replacing the culture medium of the cadaveric beetle laboratory every 3-5 days to prevent the growth of mites and fungi. It was found that adult and larval beetles and beetle larvae grew well in the culture medium and the rearing environment provided in this example. In order to collect growth and development data and body length data of the bark beetle under the condition of constant temperature of 25 ℃, the following experimental operations are carried out.
After the adult albopictus beetle has lived approximately 30 days in the simple raising device of addicting to the dead body beetle laboratory, the male and female adult copulation phenomenon appears, at this moment, regard as the ovum collection ware with a circular plastic disc that the rabbit corpse was held to the inside, put into the culture box 5 of raising the addictive to the dead body beetle of albopictus beetle with the ovum collection ware, in observing the 4 hours after laying eggs for the first time, collect 300 ~ 400 eggs, take out the egg of laying and place in a bottom includes the plastic culture dish of moist white paper handkerchief. The plastic dishes containing the eggs of the bark beetle were placed on the bottom of an insect incubator covered with a wet paper towel, and the insect incubator containing the eggs of the bark beetle was incubated in a light-shielded or low-light artificial climatic chamber at a constant temperature of 25 ℃ and a humidity of 75%. This time was considered the start time of the egg phase and was noted as 0 h. And observing the situation of eggs once every 24 hours, after 6 days, immediately transferring 200-300 eggs into a simple feeding device of a cadaveric beetle laboratory in the same constant-temperature 25 ℃ artificial climate box for feeding after observing that the eggs are hatched into larvae, and feeding the larvae into a feed box to feed the bodies of the rabbits so as to meet the growth requirement of the albopictus. The hatching rate of eggs is about 60 percent approximately, the hatching rate is recorded as the end time of the egg phase of the bark beetle and is also the starting time of the larva phase, the development condition of the bark beetle larva is observed every 24 hours from the starting time of the larva phase of the bark beetle, the corpse of a rabbit is fed in a feed box, simultaneously, 8 larvae are randomly selected from a raised larva raising device, the larvae are placed in a thin and long transparent glass tube, then, the body length of the larvae is measured by using an origin type digital display caliper, the average value is taken for measuring three times each time and is taken as the body length value of the larvae at the moment, and the measured larvae are placed back to the raising device to be continuously raised. Along with the development of the larvae, the living space in the same breeding device can be reduced, and at the moment, the developing larvae can be separately packaged into a simple breeding device of a cadaveric beetle laboratory in another constant-temperature 25 ℃ insect artificial climate box for breeding. After 30 days, 180-250 larvae are observed to pupate, the end of the larval stage is marked, the larvae enter the pupal stage, the sampling and the measurement of the larvae are stopped, and meanwhile, the feeding of food is stopped. The pupation rate is about 90% due to the large feeding space. After the pupa stage begins, the pupa of the albopictus beetle is screened out from the culture medium by a screen, the screened pupa is placed in the same constant-temperature 25 ℃ artificial climate box for further development, and the development condition of the albopictus beetle pupa is observed every 24 hours. After 10 days, the end of pupal stage, which is the time of emergence, was recorded and marked the end of the developmental history until about 150-200 bark beetles were observed to complete the development of pupal stage and emerged as adults from the pupal shell. The eclosion rate was about 80%.
In the embodiment, the egg stage, the larva stage, the pupal stage and the total development stage of the bark beetle are respectively 6 +/-2 days, 30 +/-5 days, 10 +/-3 days and 46 +/-6 days under the condition of constant temperature of 25 ℃. The length of the larvae is 5-18mm, and the length gradually increases to the longest from the beginning of larval stage and then gradually decreases until pupation is completed, and the result is shown in FIG. 3.
While the foregoing is directed to the preferred embodiment of the present invention, it will be understood by those skilled in the art that various changes and modifications may be made without departing from the spirit and scope of the invention as defined in the appended claims.
Claims (10)
1. A collection and laboratory feeding method of cadaveric beetles is characterized by comprising the following steps:
step 1, collecting adult or larva of cadaveric beetles in the field:
the rotten meat is used as a bait, and the simple and easy cadaveric beetle trapping device is used for trapping the adults or larvae of the cadaveric beetles;
step 2, preparing a culture medium and a feed for cadaveric beetles in a laboratory:
fine sand, humus, sawdust and weeds are used as laboratory culture substrates to manufacture culture boxes, and fly larvae or pupae and putrefactive later-stage corpses are used as feeds to manufacture feed boxes;
step 3, feeding cadaveric beetles in a laboratory:
putting the culture box and the feed box manufactured in the step 2 into a shading or low-light environment with the temperature of 25 ℃ and the humidity of 75% for feeding the autophagous beetles;
step 4, hatching, molting, pupating, eclosion and passage of the autophagous beetles;
step 5, collecting development data of each life stage of the autophagous beetles;
and 6, measuring the body length of the larvae of the autophagous beetles.
2. The method for collecting and laboratory feeding of cadaveric beetles according to claim 1, wherein in the step 1, the specific method for collecting the adults or larvae of the cadaveric beetles in the field is as follows: the simple and easy trapping device for the cadaveric beetles is placed on a wild grassland or a forest where the cadaveric beetles usually appear, rotten meat is placed in the simple and easy trapping device and serves as food for trapping the beetles, after 5-7 days, the cadaveric beetles collected in the device are observed and collected, and then the cadaveric beetles are brought back to a laboratory for further breeding.
3. The method for collecting and laboratory feeding of cadaveric beetles according to claim 2, wherein the cadaveric beetle trapping devices are placed in a place protected from direct sunlight while avoiding rainwater accumulation.
4. The method for collecting and feeding cadaveric beetles in a laboratory according to claim 3, wherein the device for simply trapping cadaveric beetles comprises an iron cage, a black plastic bag and a bait basin, the iron cage comprises a frame and six-sided wire netting, four corners of the bottom surface of the iron cage are provided with protruding nails, the nails can be nailed into soil during placement so as to prevent the cage from being blown down by wind or being knocked down by wild animals, the frame adopts heavy steel bars to increase the weight of the cage, wire netting gaps can allow the beetles to enter and can prevent food inside the cage from being eaten by animals larger than the beetles in volume, the iron net on the top of the iron cage can be opened, the black plastic bag is opened upwards, the upper end of the black plastic bag is fixed on the four corners of the top of the cage, the bait is placed inside the black plastic bag, and fine sand is spread at the bottom of the bait basin.
5. The method for collecting and feeding cadaveric beetles in a laboratory according to claim 4, wherein in the step 2, the specific method for preparing the culture medium and the feed for the cadaveric beetles in the laboratory is as follows:
step 21, preparation of culture medium: laying a layer of sterilized fine sand in a culture box, placing a layer of humus or leaf rotting soil on the fine sand, then placing a layer of wet fermented wood chips, placing a layer of weeds on the wood chips to form a culture medium or a hiding place of the cadaveric beetle, and regularly spraying water by using a kettle to keep a wet living environment of the beetle;
step 22, preparing the feed: the laboratory feed for the cadaveric beetles is mainly divided into 2 types, wherein the first type takes fly larvae or pupae as feed and is mainly used for feeding the cadaveric beetles in the families of buried beetles or cryptopteridae; the second kind is with putrefactive later stage corpse as the fodder, including the corpse of rat or rabbit, mainly used raises the pissodidae or Guogonga beetle of addict of cadaveric sex, but the corpse can wholly air-dry and put into the feed box, perhaps air-dries after smashing with the mixer and puts into the feed box.
6. The method for collecting and laboratory feeding of cadaveric beetles according to claim 5, wherein in the step 3, the specific method for laboratory feeding of cadaveric beetles is as follows: firstly, the collected cadaveric beetles in the step 1 are placed into a simple cadaveric beetle laboratory feeding device with the culture box and the feed box arranged inside, then the simple cadaveric beetle laboratory feeding device is placed into an insect artificial climate box with the temperature of 25 ℃, the humidity of 75%, shading or weak light for feeding, the feed prepared in the step 2 is observed and fed regularly, water is sprayed regularly to meet the growth requirement of the cadaveric beetles, and the culture medium of the cadaveric beetle laboratory is replaced every 3-5 intervals to prevent the growth of mites and fungi.
7. The method for collecting and feeding cadaveric beetles in the laboratory according to claim 6, wherein in the step 3, the simple feeding device for the cadaveric beetles in the laboratory comprises a detachable cage, six faces of the cage are connected by nylon nets, one side face of the cage is provided with a zipper, the cage is internally provided with the culture box and the feed box manufactured in the step 2, the feed box is positioned at one corner in the culture box, and the feed box is a culture dish or a plastic dish.
8. The method for collecting and laboratory feeding of cadaveric beetles according to claim 7, wherein the specific methods of hatching, molting, pupating, eclosion and passaging in step 4 are as follows: when the male and female adults reach sexual maturity, namely the male and female adults are observed to have mating behaviors, putting an egg collector containing rotten meat into the culture box, taking out the born cadaveric beetle eggs and placing the cadaveric beetle eggs into a plastic culture dish of which the bottom comprises wet white paper towels within 4 hours after the eggs are observed for the first time, then placing the plastic culture dish containing the cadaveric beetle eggs into the bottom of an insect incubator covered by wet paper towels, and finally placing the insect incubator into an insect student artificial climate box with the constant temperature of 25 ℃, the humidity of 75% and shading or weak light for incubation; after eggs are hatched into larvae, immediately transferring the larvae from a plastic culture dish into another simple breeding device of a cadaveric beetle laboratory in the same climatic chamber for breeding, after the larvae are drilled into a culture medium to be pupated, screening the cadaveric beetle pupae out of the culture medium by using a screen, sterilizing the screened culture medium and then repeatedly using the culture medium, placing the screened beetle pupae into an open plastic box, then placing the box at the bottom of an insect box covered by a wet tissue, and finally placing the insect box filled with the cadaveric beetle pupae into an insect study artificial climatic chamber with constant temperature of 25 ℃, humidity of 75 percent and shading or weak light for continuous development; after the pupae of the autophagous beetle eclosion into adults, the adults are transferred into another simple breeding device in an autophagous beetle laboratory for breeding, after the adults are mature sexually, spawning is induced, one passage is completed, and then the breeding is repeated.
9. The method for collecting and feeding cadaveric beetles in a laboratory according to claim 8, wherein the step 5 is implemented by collecting development data of each life stage of the cadaveric beetles by the following specific method: recording the time when the same batch of cadaveric beetle eggs are placed in an insect incubator as the start time of an egg phase, recording the end time of the egg phase of the beetle when more than 50% of the cadaveric beetle eggs are observed to be incubated into larvae, and simultaneously recording the start time of the larva phase, recording the start time of the pupal phase when more than 50% of the larvae are observed to be pupated, and recording the end of the pupal phase when more than 50% of the cadaveric beetle pupae are adult, wherein the end of the development history phase is marked.
10. The method for collecting and laboratory feeding of cadaveric beetles according to claim 9, wherein the specific method for measuring the body length of each larval stage of the cadaveric beetles in step 6 is as follows: randomly selecting 5-10 larvae every 24 hours from the beginning time of a larva stage, putting the larvae into a thin and long transparent glass tube, measuring the body length of the larvae by using an original point type digital display caliper, measuring the average value of the body length of the larvae for three times to serve as the body length value of the larvae at the moment, and putting the measured larvae back into a breeding device for continuous breeding; when more than 50% of the larvae have completed pupation, the larval stage is marked to the end, at which time sampling and measurement of the larvae is stopped.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113875705A (en) * | 2021-11-01 | 2022-01-04 | 中南大学 | Collection and culture method of autophagic drosophila |
| CN114868708A (en) * | 2022-04-15 | 2022-08-09 | 中南大学 | Breeding and domesticating method for Boettcherisca peregrina |
| CN116694781A (en) * | 2023-07-04 | 2023-09-05 | 苏州大学 | Method for identifying cadaveric beetle species by combining mitochondrial gene COI and nuclear gene 28SrDNA |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN206314455U (en) * | 2016-11-14 | 2017-07-11 | 李学博 | A kind of forensic entomology sample trap |
| CN207201775U (en) * | 2017-09-06 | 2018-04-10 | 中南大学 | A kind of thermophilic corpse fly larvae rearging cage of counting type |
| DE202018102935U1 (en) * | 2018-04-26 | 2018-06-13 | Witasek Pflanzenschutz Gmbh | Collection container for slot trap |
| CN109122597A (en) * | 2018-10-23 | 2019-01-04 | 广东省生物资源应用研究所 | A kind of method for breeding of the small beetle of beehive |
| CN109287574A (en) * | 2018-11-19 | 2019-02-01 | 华南农业大学 | Dendroctonus armandi hand mating method for breeding |
-
2020
- 2020-06-10 CN CN202010525262.5A patent/CN111480621B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN206314455U (en) * | 2016-11-14 | 2017-07-11 | 李学博 | A kind of forensic entomology sample trap |
| CN207201775U (en) * | 2017-09-06 | 2018-04-10 | 中南大学 | A kind of thermophilic corpse fly larvae rearging cage of counting type |
| DE202018102935U1 (en) * | 2018-04-26 | 2018-06-13 | Witasek Pflanzenschutz Gmbh | Collection container for slot trap |
| CN109122597A (en) * | 2018-10-23 | 2019-01-04 | 广东省生物资源应用研究所 | A kind of method for breeding of the small beetle of beehive |
| CN109287574A (en) * | 2018-11-19 | 2019-02-01 | 华南农业大学 | Dendroctonus armandi hand mating method for breeding |
Non-Patent Citations (2)
| Title |
|---|
| SHUBECK P P: "an alternative to pitfall traps in carrion beetle sutdies(coleoptera)", 《ENTOMOLOGICAL NEWS》 * |
| 罗佑珍等: "白腹皮蠹的生物学特性及温湿度对其生长的影响", 《云南农业大学学报》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113875705A (en) * | 2021-11-01 | 2022-01-04 | 中南大学 | Collection and culture method of autophagic drosophila |
| CN114868708A (en) * | 2022-04-15 | 2022-08-09 | 中南大学 | Breeding and domesticating method for Boettcherisca peregrina |
| CN116694781A (en) * | 2023-07-04 | 2023-09-05 | 苏州大学 | Method for identifying cadaveric beetle species by combining mitochondrial gene COI and nuclear gene 28SrDNA |
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