CN109880803B - 嵌合蛋白、表达嵌合蛋白的免疫效应细胞及其应用 - Google Patents

嵌合蛋白、表达嵌合蛋白的免疫效应细胞及其应用 Download PDF

Info

Publication number
CN109880803B
CN109880803B CN201811488310.7A CN201811488310A CN109880803B CN 109880803 B CN109880803 B CN 109880803B CN 201811488310 A CN201811488310 A CN 201811488310A CN 109880803 B CN109880803 B CN 109880803B
Authority
CN
China
Prior art keywords
ser
leu
gly
pro
domain
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201811488310.7A
Other languages
English (en)
Chinese (zh)
Other versions
CN109880803A (zh
Inventor
李宗海
王益
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kaixing Life Technology Shanghai Co ltd
Original Assignee
Kaixing Life Technology Shanghai Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kaixing Life Technology Shanghai Co ltd filed Critical Kaixing Life Technology Shanghai Co ltd
Publication of CN109880803A publication Critical patent/CN109880803A/zh
Application granted granted Critical
Publication of CN109880803B publication Critical patent/CN109880803B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00Medicinal preparations containing antigens or antibodies
    • A61K39/46Cellular immunotherapy
    • A61K39/464Cellular immunotherapy characterised by the antigen targeted or presented
    • A61K39/4643Vertebrate antigens
    • A61K39/4644Cancer antigens
    • A61K39/464474Proteoglycans, e.g. glypican, brevican or CSPG4
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00Medicinal preparations containing antigens or antibodies
    • A61K39/46Cellular immunotherapy
    • A61K39/461Cellular immunotherapy characterised by the cell type used
    • A61K39/4611T-cells, e.g. tumor infiltrating lymphocytes [TIL], lymphokine-activated killer cells [LAK] or regulatory T cells [Treg]
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00Medicinal preparations containing antigens or antibodies
    • A61K39/46Cellular immunotherapy
    • A61K39/462Cellular immunotherapy characterized by the effect or the function of the cells
    • A61K39/4621Cellular immunotherapy characterized by the effect or the function of the cells immunosuppressive or immunotolerising
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00Medicinal preparations containing antigens or antibodies
    • A61K39/46Cellular immunotherapy
    • A61K39/463Cellular immunotherapy characterised by recombinant expression
    • A61K39/4631Chimeric Antigen Receptors [CAR]
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00Antineoplastic agents
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K19/00Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10Cells modified by introduction of foreign genetic material
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2239/00Indexing codes associated with cellular immunotherapy of group A61K39/46
    • A61K2239/27Indexing codes associated with cellular immunotherapy of group A61K39/46 characterized by targeting or presenting multiple antigens
    • A61K2239/28Expressing multiple CARs, TCRs or antigens
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2239/00Indexing codes associated with cellular immunotherapy of group A61K39/46
    • A61K2239/46Indexing codes associated with cellular immunotherapy of group A61K39/46 characterised by the cancer treated
    • A61K2239/53Liver
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503Immunoglobulin superfamily
    • C07K14/7051T-cell receptor (TcR)-CD3 complex
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/03Fusion polypeptide containing a localisation/targetting motif containing a transmembrane segment

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Organic Chemistry (AREA)
  • Cell Biology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Microbiology (AREA)
  • Epidemiology (AREA)
  • Immunology (AREA)
  • Mycology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Oncology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Communicable Diseases (AREA)
  • Peptides Or Proteins (AREA)
CN201811488310.7A 2017-12-06 2018-12-06 嵌合蛋白、表达嵌合蛋白的免疫效应细胞及其应用 Active CN109880803B (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN2017112797051 2017-12-06
CN201711279705 2017-12-06

Publications (2)

Publication Number Publication Date
CN109880803A CN109880803A (zh) 2019-06-14
CN109880803B true CN109880803B (zh) 2024-06-07

Family

ID=66750790

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201811488310.7A Active CN109880803B (zh) 2017-12-06 2018-12-06 嵌合蛋白、表达嵌合蛋白的免疫效应细胞及其应用

Country Status (2)

Country Link
CN (1) CN109880803B (fr)
WO (1) WO2019109980A1 (fr)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20210025525A (ko) 2018-06-22 2021-03-09 카이트 파마 인코포레이티드 키메라 막횡단 단백질 및 그의 용도
BR112021025735A2 (pt) * 2019-06-21 2022-03-08 Kite Pharma Inc Receptores de tgf-ss e métodos de uso
CN114929863A (zh) * 2019-08-09 2022-08-19 克莱格医学有限公司 共表达趋化因子受体的免疫效应细胞
CN112390892B (zh) * 2019-08-14 2024-01-12 博源润生医药(杭州)有限公司 一种嵌合抗原受体以及表达该嵌抗原受体的免疫效应细胞
CN110564695A (zh) * 2019-09-23 2019-12-13 华东师范大学 靶向前列腺癌的增强型car-t细胞及其制备方法和药物
JP7488337B2 (ja) * 2019-11-27 2024-05-21 ジーアイ・セル・インコーポレイテッド Il-2タンパク質及びcd80タンパク質を含む融合タンパク質及びnk細胞を含む抗癌治療用組成物
CN111171160B (zh) * 2020-02-14 2022-05-17 四川大学华西医院 基于TGF-β改造的嵌合抗原受体及其修饰的免疫细胞
CN111218426A (zh) * 2020-02-26 2020-06-02 华东师范大学 一种克服肿瘤微环境car-t细胞的制备及其用途
CN111269326A (zh) * 2020-02-28 2020-06-12 南京北恒生物科技有限公司 新型嵌合抗原受体及其用途
CN111690070A (zh) * 2020-05-13 2020-09-22 深圳市众循精准医学研究院 一种sPD-1-Fc-sTGFβRII融合蛋白及其应用
CN115698038A (zh) * 2020-06-01 2023-02-03 上海君赛生物科技有限公司 信号转换受体及其用途
CN112210543B (zh) * 2020-07-30 2023-01-03 山东省成体细胞产业技术研究院有限公司 一种针对实体瘤克服TGF-β免疫抑制的CAR-T细胞
CN116194125A (zh) 2020-08-07 2023-05-30 克莱格医学有限公司 工程化改造的细胞以及工程化改造细胞的方法
CN111978412B (zh) * 2020-08-13 2021-04-30 南京凯地生物科技有限公司 武装靶向TGF-β的特异性嵌合抗原受体细胞及其制备方法和应用
CN116390940A (zh) * 2020-09-23 2023-07-04 海正生物制药有限公司 Tgfbr2-ecd突变体及包含其的融合蛋白与应用
CN117545771A (zh) 2021-04-08 2024-02-09 克莱格医学有限公司 细胞免疫治疗的应用
CN117460741A (zh) 2021-06-29 2024-01-26 科济生物医药(上海)有限公司 调控细胞生理活动的嵌合多肽
TW202317602A (zh) * 2021-07-15 2023-05-01 福瑞德哈金森腫瘤中心 嵌合多肽
CN115873802A (zh) * 2021-09-29 2023-03-31 亘喜生物科技(上海)有限公司 嵌合抗原受体免疫细胞及其制法和应用
WO2024099990A1 (fr) * 2022-11-07 2024-05-16 Leibniz-Institut Für Immuntherapie (Lit) Cellules car t de récepteur de commutateur tgf-ss

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103582699A (zh) * 2011-04-08 2014-02-12 贝勒医学院 使用嵌合细胞因子受体逆转肿瘤微环境的影响
WO2016061574A1 (fr) * 2014-10-17 2016-04-21 Baylor College Of Medicine Cellules immunitaires bipartites et tripartites de signalisation
WO2016122738A1 (fr) * 2015-01-31 2016-08-04 The Trustees Of The University Of Pennsylvania Compositions et procédés d'administration à des cellules t de molécules thérapeutiques

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3939572B1 (fr) * 2012-04-12 2024-03-27 Yale University Véhicules pour l'administration contrôlée d'agents pharmaceutiques différents
JP6578271B2 (ja) * 2013-04-17 2019-09-18 ベイラー カレッジ オブ メディスンBaylor College Of Medicine 免疫抑制TGF−βシグナルコンバーター

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103582699A (zh) * 2011-04-08 2014-02-12 贝勒医学院 使用嵌合细胞因子受体逆转肿瘤微环境的影响
WO2016061574A1 (fr) * 2014-10-17 2016-04-21 Baylor College Of Medicine Cellules immunitaires bipartites et tripartites de signalisation
WO2016122738A1 (fr) * 2015-01-31 2016-08-04 The Trustees Of The University Of Pennsylvania Compositions et procédés d'administration à des cellules t de molécules thérapeutiques

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Benjamin Boyerinas Boyerinas et al..A novel TGF-β/IL-12R signal conversion platform that protects CAR T cells from TGF-β-mediated immune suppression and concurrently amplifies effector function.Cancer Res.2017,第77卷(第13_supplement期),1. *

Also Published As

Publication number Publication date
CN109880803A (zh) 2019-06-14
WO2019109980A1 (fr) 2019-06-13

Similar Documents

Publication Publication Date Title
CN109880803B (zh) 嵌合蛋白、表达嵌合蛋白的免疫效应细胞及其应用
JP7008350B2 (ja) Car発現ベクター及びcar発現t細胞
EP3586852B1 (fr) Expansion de cellules modifiées et leurs utilisations
Ahmed et al. HER2-specific T cells target primary glioblastoma stem cells and induce regression of autologous experimental tumors
JP6285553B2 (ja) 抗cd30キメラ抗原受容体およびその使用
WO2017219936A1 (fr) Lymphocyte t exprimant le récepteur car capable d'exprimer avec efficacité et stabilité un anticorps activé, et ses utilisations
JP6761113B2 (ja) キメラ抗原受容体
WO2019052562A1 (fr) Protéine de fusion d'une il-4r et son utilisation
TWI811278B (zh) 表現特異性辨識人類間皮素之細胞表面分子、il-7、及ccl19之免疫活性細胞
CN113677353A (zh) 修饰细胞的扩增及其用途
CN113692441A (zh) 一种包含肿瘤抗原识别受体的免疫细胞及其应用
CN114867490A (zh) 细胞因子调控表达的免疫效应细胞
WO2020034081A1 (fr) Récepteur d'antigène chimère visant bcma et ses utilisations
CN116514998B (zh) 一种嵌合抗原受体、嵌合抗原受体-自然杀伤细胞及其在制备抗肿瘤药物中的应用
WO2024055339A1 (fr) Procédé de préparation et d'amplification d'une cellule car-nk anti-cd19 humanisée universelle et son utilisation
US20220380433A1 (en) Tmem59 protein dimer or chimeric expression receptor improving t cell function
WO2023286841A1 (fr) Récepteur antigénique chimérique, cellule exprimant ledit récepteur, composition pharmaceutique contenant ladite cellule, procédé de fabrication de ladite cellule, et polynucléotide ou vecteur contenant une séquence de bases codant ledit récepteur antigénique chimérique
CN111548999A (zh) 调节淋巴细胞cd44途径的修饰细胞及其用途
CN115704039A (zh) 包含编码抗原结合分子的多核苷酸和靶向ecm试剂的多核苷酸的多核苷酸及修饰细胞
CN111607596A (zh) 修饰的pd-1及其在细胞治疗中的用途
NZ757552B2 (en) Chimeric antigen receptor

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
TA01 Transfer of patent application right

Effective date of registration: 20210914

Address after: Ai Erlandubailin

Applicant after: Fufa Pharmaceutical Co.,Ltd.

Address before: Building B, 388 Yindu Road, Xuhui District, Shanghai, 200231

Applicant before: Keji biomedical (Shanghai) Co.,Ltd.

Applicant before: SHANGHAI CANCER INSTITUTE

TA01 Transfer of patent application right
TA01 Transfer of patent application right

Effective date of registration: 20220128

Address after: Room 12, 20th floor, Haojing commercial center, 2-16 Huayuan street, Mongkok, Kowloon, China

Applicant after: Clegg Medical Co.,Ltd.

Address before: Irish Dublin

Applicant before: Fufa Pharmaceutical Co.,Ltd.

TA01 Transfer of patent application right
TA01 Transfer of patent application right

Effective date of registration: 20240509

Address after: Room 303, 3rd Floor, No. 203 Fute North Road, China (Shanghai) Pilot Free Trade Zone, Pudong New Area, Shanghai

Applicant after: Kaixing Life Technology (Shanghai) Co.,Ltd.

Country or region after: China

Address before: Room 12, 20th Floor, Haojing Commercial Centre, 2-16 Garden Street, Mong Kok, Kowloon, Hong Kong, China

Applicant before: Clegg Medical Co.,Ltd.

Country or region before: Hong-Kong

TA01 Transfer of patent application right
GR01 Patent grant