CN109846757B - Sebum biomimetic composition and cosmetic containing same - Google Patents
Sebum biomimetic composition and cosmetic containing same Download PDFInfo
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- CN109846757B CN109846757B CN201910117675.7A CN201910117675A CN109846757B CN 109846757 B CN109846757 B CN 109846757B CN 201910117675 A CN201910117675 A CN 201910117675A CN 109846757 B CN109846757 B CN 109846757B
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Abstract
The invention provides a sebum bionic composition and a cosmetic containing the sebum bionic composition, wherein the sebum bionic composition comprises jojoba oil, argania spinosa kernel oil, seabuckthorn fruit oil, plant squalane and chinquapin oil, and the cosmetic comprises the sebum bionic composition provided by the invention.
Description
Technical Field
The invention relates to the technical field of cosmetics, in particular to a sebum biomimetic composition and a cosmetic containing the same.
Background
The skin of a human body is damaged from outside at any moment, and due to dryness, aging, improper skin care products or excessive cleaning, the sebum on the surface of the skin is produced too little or is deprived in a large amount, so that the barrier function of the skin is reduced, the moisture retaining capacity of the skin is reduced, and dryness, sensitivity, pruritus and the like are easily caused. Meanwhile, harmful bacteria can also increase and propagate to cause infection, the cell differentiation capacity is affected, the cutin is not regularly arranged, subcutaneous collagen is lost, and various skin problems are caused. Research shows that most skin problems are caused by the damage of the barrier function of the skin, the barrier function of the horny layer is damaged, the tolerance or the defense capability of the skin is reduced, and the skin problems of dry skin, fragile and sensitive skin, easy external infection and the like are caused.
The skin epidermis barrier is formed by skin surface sebum membrane, intercellular lipid forming brick wall structure, dermis mucopolysaccharide, mucopolysaccharide and other components, and resists the entry of harmful and irritants outside, wherein the sebum is used as the outermost layer component of the skin, and the main components of the sebum comprise triglyceride, free fatty acid, wax ester, squalene, cholesterol ester, cholesterol and other components, and the skin epidermis barrier is vital to the function of the skin barrier, and the skin epidermis barrier mainly has the following functions: 1. the water loss of the skin is prevented, and a lipid film is formed on the surface of the skin by the lipid layer, so that the horny layer is soft and moist, excessive evaporation of water in the skin can be prevented, and the moisture-keeping effect is achieved; 2. the damage of ultraviolet rays to the skin is reduced, and as the outermost layer of the skin, the integrity of the sebum layer plays a vital role in reducing the damage of the ultraviolet rays to the skin; 3. protecting against biological attack, triglyceride secreted from sebum layer is decomposed under the action of esterase to generate free fatty acid (such as undecylenic acid) which can inhibit the growth and reproduction of Staphylococcus aureus, hemolytic streptococcus, Candida albicans, etc. The sebaceous layer therefore plays a crucial role in the skin barrier function.
At present, a variety of skin barrier function repairing cosmetics are provided, inflammation of damaged skin is reduced by enhancing the moisture retention of the skin surface layer or adding a soothing component, and the effects of barrier function damage and sebum loss cannot be fundamentally solved.
CN105748315B discloses a composition for repairing skin barrier, which is prepared from the following raw materials by weight: 0.1-1% of sodium hyaluronate, 0.5-3% of trehalose, 5-25% of humectant and 71-94.4% of water. Experiments prove that the composition for repairing the skin barrier can effectively relieve the symptoms of red swelling, hot pain, skin fading, pruritus and the like caused by skin after the beauty means such as laser beauty, tartaric acid skin changing and the like are treated, has good absorption effect, simple preparation method, short production period, simple prescription and low cost, but can only play the roles of relieving and moisturizing, does not fundamentally repair the skin barrier and reduce the loss of sebum.
CN108685741A discloses a composition for promoting skin barrier repair and an application thereof, wherein the composition for promoting skin barrier repair comprises the following components in parts by weight: 10-20 wt% of trehalose, 10-30 wt% of sodium hyaluronate, 5-15 wt% of centella asiatica extract, 5-15 wt% of spirulina polysaccharide, 5-15 wt% of collagen, 5-20 wt% of aloe extract, 1-5 wt% of ceramide, 1-5 wt% of sodium polyglutamate, 1-2 wt% of ascorbyl glucoside and 0.5-2 wt% of acetyl chitosamine. The composition prepared by the invention can be used for promoting the repair of the damaged skin barrier, and is particularly suitable for promoting the repair of the damaged skin barrier after laser beauty treatment, but the composition only can play the roles of moisturizing, relieving and diminishing inflammation, and the problem of damaged skin barrier is not fundamentally solved.
CN108498371A discloses a composition with skin barrier repairing effect and its preparation method, wherein the raw materials in the composition include mannitol and glycosphingolipids, and the composition is further researched to be: mannitol, glycosphingolipids, glycerophosphoinositide choline salt, tetrahydromethylpyrimidine carboxylic acid, oligopeptide-1; researches show that the composition has good effect of repairing skin barriers, but the composition only can temporarily keep moisture and relieve the skin, and does not fundamentally repair the skin barriers and slow the loss of sebum.
Therefore, in view of the fundamental problem of repairing skin barrier, those skilled in the art should develop a composition and cosmetic, which not only has better effects of relieving inflammation, but also has better effects of repairing skin barrier and promoting cell regeneration.
Disclosure of Invention
The invention aims to provide a sebum bionic composition which comprises jojoba oil, acarpus spinosus kernel oil, seabuckthorn fruit oil, plant squalane and chinaru chinensis seed oil.
Jojoba oil, known as desert liquid gold, contains various natural unsaturated fatty acids, vitamin E and other components, is very close to human sebum, has excellent permeability to skin, and naturally nourishes the skin.
Argan kernel oil, also known as argan, is extracted from nuts grown on argan tree in morocco, and contains a large amount of fatty acids, diglycerides, triglycerides, sterols, vitamin E, and phenolic compounds. It contains a large amount of essential fatty acid, unsaturated fatty acid and linoleic acid. The fatty acid maintains the hydration degree of human health, linoleic acid has the effects of resisting inflammation, reducing acne and the like, the unsaturated fatty acid slows down the premature aging and wrinkles of the skin, and also contains rare phytosterol, and the phytosterol can help to soften the skin, stimulate pores to expel toxin and restore the natural lipid barrier.
The seabuckthorn fruit oil contains linolenic acid, linoleic acid and other unsaturated fatty acids with the content as high as 70 percent, contains diglyceride, triglyceride, carotenoid, vitamin E and various trace elements which are indispensable to human bodies, such as vitamin A, selenium, magnesium, zinc, iron and manganese, and in addition, a small amount of phytosterol, catechins, lecithin and flavonoid compounds in the seabuckthorn fruit oil, and the seabuckthorn fruit oil has multiple effects of resisting free radicals, removing freckles, repairing cuticles and the like in cosmetics.
The plant squalane is obtained by hydrogenation of squalene from natural olive oil, the molecular structure of the plant squalane is similar to that of squalene in a skin lipid layer, and the plant squalane has few advantages of high chemical stability and good affinity to skin, can be integrated with a skin lipid film of human, and forms a layer of natural barrier on the surface of the skin. Can also inhibit peroxidation of skin lipid, effectively permeate into skin, promote proliferation of basal cells of skin, delay skin aging, and help repair damaged stratum corneum.
The meadowrue root oil is called as a natural antioxidant, contains a large amount of vitamin E, can adjust the balance of skin, water, oil and NMF moisturizing factors, improves the self-resistance of the skin, and keeps the skin soft for a long time.
The main components of the sebum layer comprise: 41% of diglyceride, 2.2% of triglyceride, 16.4% of fatty acid, 12.0% of squalene, 20.5% of phospholipid, 1.4% of cholesterol and 2.1% of cholesterol ester, and the four components of diglyceride, triglyceride, fatty acid and squalene are the most main components playing a role in skin barrier effect.
The invention provides a composition containing five natural vegetable oils, namely jojoba oil, argania spinosa kernel oil, sea buckthorn fruit oil, plant squalane and chinaroot greenbrier seed oil, and the composition provided by the invention contains the four main components playing a role in skin barrier, and contains nutrient substances such as vitamin E and sterol at the same time, and the components are matched with each other, so that the composition can simulate the composition structure of human body sebum, has the functions of relieving and resisting inflammation, can form a layer of rawhide lipid layer on the surface of skin, effectively resists external damage, plays a good role in repairing the skin barrier and promoting cell regeneration, fundamentally solves the problem of damaged skin barrier, and finally achieves the effects of moisturizing, relieving, resisting aging, restoring skin elasticity and the like.
Preferably, the sebum bionic composition comprises the following components in parts by weight:
furthermore, the skin care product selects five natural vegetable oils to be combined according to a specific proportion, more accurately simulates the sebum composition structure of a human body, can further improve the capabilities of repairing skin barriers and promoting cell regeneration, and achieves better effects of moisturizing, soothing, resisting aging, restoring skin elasticity and the like.
The sebum biomimetic composition comprises 1-40 parts by weight of jojoba oil, such as 2 parts by weight, 3 parts by weight, 4 parts by weight, 5 parts by weight, 6 parts by weight, 7 parts by weight, 8 parts by weight, 9 parts by weight, 10 parts by weight, 11 parts by weight, 12 parts by weight, 13 parts by weight, 14 parts by weight, 15 parts by weight, 16 parts by weight, 17 parts by weight, 18 parts by weight, 19 parts by weight, 20 parts by weight, 21 parts by weight, 22 parts by weight, 23 parts by weight, 24 parts by weight, 25 parts by weight, 26 parts by weight, 27 parts by weight, 28 parts by weight, 29 parts by weight, 30 parts by weight, 31 parts by weight, 32 parts by weight, 33 parts by weight, 34 parts by weight, 35 parts by weight, 36 parts by weight, 37 parts by weight, 38 parts by weight, 39 parts by weight, and the like.
The sebum-simulating composition comprises 1-50 parts by weight of argania spinosa kernel oil, such as 2 parts by weight, 3 parts by weight, 4 parts by weight, 5 parts by weight, 6 parts by weight, 7 parts by weight, 8 parts by weight, 9 parts by weight, 10 parts by weight, 11 parts by weight, 12 parts by weight, 13 parts by weight, 14 parts by weight, 15 parts by weight, 16 parts by weight, 17 parts by weight, 18 parts by weight, 19 parts by weight, 20 parts by weight, 21 parts by weight, 22 parts by weight, 23 parts by weight, 24 parts by weight, 25 parts by weight, 26 parts by weight, 27 parts by weight, 28 parts by weight, 29 parts by weight, 30 parts by weight, 31 parts by weight, 32 parts by weight, 33 parts by weight, 34 parts by weight, 35 parts by weight, 36 parts by weight, 37 parts by weight, 38 parts by weight, 39 parts by weight, 40 parts by weight, 41 parts by weight, 42 parts by weight, 43 parts by weight, 44 parts by weight, 45 parts by weight, 46 parts by weight, 47 parts by weight, 48 parts by weight and 49 parts by weight, and the like.
The sebum biomimetic composition comprises 1-25 parts by weight of seabuckthorn fruit oil, such as 2 parts by weight, 3 parts by weight, 4 parts by weight, 5 parts by weight, 6 parts by weight, 7 parts by weight, 8 parts by weight, 9 parts by weight, 10 parts by weight, 11 parts by weight, 12 parts by weight, 13 parts by weight, 14 parts by weight, 15 parts by weight, 16 parts by weight, 17 parts by weight, 18 parts by weight, 19 parts by weight, 20 parts by weight, 21 parts by weight, 22 parts by weight, 23 parts by weight, 24 parts by weight, and the like.
The sebum biomimetic composition comprises 1-40 parts by weight of plant squalane, such as 2 parts by weight, 3 parts by weight, 4 parts by weight, 5 parts by weight, 6 parts by weight, 7 parts by weight, 8 parts by weight, 9 parts by weight, 10 parts by weight, 11 parts by weight, 12 parts by weight, 13 parts by weight, 14 parts by weight, 15 parts by weight, 16 parts by weight, 17 parts by weight, 18 parts by weight, 19 parts by weight, 20 parts by weight, 21 parts by weight, 22 parts by weight, 23 parts by weight, 24 parts by weight, 25 parts by weight, 26 parts by weight, 27 parts by weight, 28 parts by weight, 29 parts by weight, 30 parts by weight, 31 parts by weight, 32 parts by weight, 33 parts by weight, 34 parts by weight, 35 parts by weight, 36 parts by weight, 37 parts by weight, 38 parts by weight, 39 parts by weight, and the like.
The sebum biomimetic composition comprises 1-40 parts by weight of meadowfoam seed oil, such as 2 parts by weight, 3 parts by weight, 4 parts by weight, 5 parts by weight, 6 parts by weight, 7 parts by weight, 8 parts by weight, 9 parts by weight, 10 parts by weight, 11 parts by weight, 12 parts by weight, 13 parts by weight, 14 parts by weight, 15 parts by weight, 16 parts by weight, 17 parts by weight, 18 parts by weight, 19 parts by weight, 20 parts by weight, 21 parts by weight, 22 parts by weight, 23 parts by weight, 24 parts by weight, 25 parts by weight, 26 parts by weight, 27 parts by weight, 28 parts by weight, 29 parts by weight, 30 parts by weight, 31 parts by weight, 32 parts by weight, 33 parts by weight, 34 parts by weight, 35 parts by weight, 36 parts by weight, 37 parts by weight, 38 parts by weight, 39 parts by weight, and the like.
Preferably, the sebum bionic composition comprises the following components in parts by weight:
five kinds of natural vegetable oil are further selected to be combined according to the proportion, so that the sebum composition structure of the human body is simulated more accurately, the capabilities of repairing skin barriers and promoting cell regeneration can be further improved, and the effects of better moisturizing, soothing, anti-aging, skin elasticity recovery and the like are achieved.
Preferably, the sebum bionic composition comprises the following components in parts by weight:
five kinds of natural vegetable oil are further selected to be combined according to the proportion, so that the sebum composition structure of the human body is simulated more accurately, the capabilities of repairing skin barriers and promoting cell regeneration can be further improved, and the effects of better moisturizing, soothing, anti-aging, skin elasticity recovery and the like are achieved.
Preferably, the sebum bionic composition comprises the following components in parts by weight:
five kinds of natural vegetable oil are further selected to be combined according to the proportion, so that the sebum composition structure of the human body is simulated more accurately, the capabilities of repairing skin barriers and promoting cell regeneration can be further improved, and the effects of better moisturizing, soothing, anti-aging, skin elasticity recovery and the like are achieved.
The second object of the present invention is to provide a cosmetic comprising the sebum-mimicking composition of the first object.
Preferably, the cosmetic includes any one of a moisturizing cream, a lotion, an essence oil, and a mask patch.
Preferably, the mass percentage of the sebum-mimicking composition in the cosmetic is 0.01-30%, such as 0.02%, 0.05%, 0.1%, 0.5%, 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5%, 5.5%, 6%, 6.5%, 7%, 7.5%, 8%, 8.5%, 9%, 9.5%, 10%, 10.5%, 11%, 11.5%, 12%, 12.5%, 13%, 13.5%, 14%, 14.5%, 15%, 15.5%, 16%, 16.5%, 17%, 17.5%, 18%, 18.5%, 19%, 19.5%, 20%, 20.5%, 21%, 21.5%, 22%, 22.5%, 23%, 23.5%, 24%, 24.5%, 25%, 25.5%, 26%, 26.5%, 27%, 27.5%, 28%, 28.5%, 29.5%, 29.8%, etc.
According to the invention, 0.01-30% of the sebum bionic composition is added into the cosmetic, so that the cosmetic has the optimal capabilities of repairing skin barriers and promoting cell regeneration within the range, and the optimal effects of moisturizing, relieving, resisting aging, recovering skin elasticity and the like are achieved.
When the content of the sebum bionic composition is too low (less than 0.01 percent), the corresponding effect cannot be achieved; too high a content (more than 30%) results in too greasy cosmetic, poor feeling in use, difficulty in absorption of nutrients, and may block pores, affecting the effect of the cosmetic.
Preferably, the cosmetic is a moisturizer in which the mass percentage of the sebum biomimetic composition is 0.01-30%, such as 0.02%, 0.05%, 0.1%, 0.5%, 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5%, 5.5%, 6%, 6.5%, 7%, 7.5%, 8%, 8.5%, 9%, 9.5%, 10%, 10.5%, 11%, 11.5%, 12%, 12.5%, 13%, 13.5%, 14%, 14.5%, 15%, 15.5%, 16%, 16.5%, 17%, 17.5%, 18%, 18.5%, 19%, 19.5%, 20%, 20.5%, 21%, 21.5%, 22%, 22.5%, 23%, 23.5%, 24%, 24.5%, 25.5%, 26%, 26.5%, 27%, 27.5%, 28%, 28.5%, 29.5%, and the like.
Compared with the prior art, the invention has the following beneficial effects:
the invention provides a composition containing five natural vegetable oils, namely jojoba oil, argania spinosa kernel oil, seabuckthorn fruit oil, plant squalane and chinaroot greenbrier seed oil, wherein the composition simultaneously contains four main components playing a role in skin barrier in a skin lipid layer, and simultaneously contains nutrient substances such as vitamin E, sterol and the like, and the components are matched with each other to simulate a human body sebum composition structure, so that the composition has the functions of relieving and resisting inflammation, can form a layer of imitation rawhide lipid layer on the surface of skin, effectively resists external damage, plays a good role in repairing the skin barrier and promoting cell regeneration, fundamentally solves the problem of skin barrier damage, and finally achieves the effects of moisturizing, soothing, anti-aging, restoring skin elasticity and the like.
Drawings
FIG. 1 is a bar graph showing the improvement effect of the samples of example 11 and comparative example 11 of the present invention on the symptoms of skin itching caused by antihistamine.
FIG. 2 is a graph showing the change in skin hydration rate before and after the use of the samples of example 11 of the present invention and comparative example 11.
Detailed Description
The technical solution of the present invention is further explained by the following embodiments. It should be understood by those skilled in the art that the examples are only for the understanding of the present invention and should not be construed as the specific limitations of the present invention.
Example 1
The sebum bionic composition comprises the following components in parts by weight:
the components are mixed according to the formula amount to prepare the sebum bionic composition.
Example 2
The sebum bionic composition comprises the following components in parts by weight:
the components are mixed according to the formula amount to prepare the sebum bionic composition.
Example 3
The sebum bionic composition comprises the following components in parts by weight:
the components are mixed according to the formula amount to prepare the sebum bionic composition.
Example 4
The sebum bionic composition comprises the following components in parts by weight:
the components are mixed according to the formula amount to prepare the sebum bionic composition.
Example 5
The sebum bionic composition comprises the following components in parts by weight:
the components are mixed according to the formula amount to prepare the sebum bionic composition.
Example 6
The sebum bionic composition comprises the following components in parts by weight:
the components are mixed according to the formula amount to prepare the sebum bionic composition.
Example 7
The sebum bionic composition comprises the following components in parts by weight:
the components are mixed according to the formula amount to prepare the sebum bionic composition.
Example 8
The sebum bionic composition comprises the following components in parts by weight:
the components are mixed according to the formula amount to prepare the sebum bionic composition.
Example 9
The sebum bionic composition comprises the following components in parts by weight:
the components are mixed according to the formula amount to prepare the sebum bionic composition.
Example 10
The sebum bionic composition comprises the following components in parts by weight:
the components are mixed according to the formula amount to prepare the sebum bionic composition.
Comparative examples 1 to 5
Comparative examples 1-5 each contained only one natural vegetable oil, as shown in Table 1.
TABLE 1
| Comparative example 1 | Comparative example 2 | Comparative example 3 | Comparative example 4 | Comparative example 5 |
| Jojoba oil | Aralia elata seem kernel oil | Sea buckthorn fruit oil | Plant squalane | Potentilla chinensis seed oil |
Comparative example 6
The sebum bionic composition comprises the following components in parts by weight:
the components are mixed according to the formula amount to prepare the sebum bionic composition.
Comparative example 7
The components are mixed according to the formula amount to prepare the sebum bionic composition.
Comparative example 8
The components are mixed according to the formula amount to prepare the sebum bionic composition.
Comparative example 9
The components are mixed according to the formula amount to prepare the sebum bionic composition.
Comparative example 10
The sebum bionic composition comprises the following components in parts by weight:
the components are mixed according to the formula amount to prepare the sebum bionic composition.
Example 11
A moisturizing cream comprising the sebum biomimetic composition provided by the invention, and the formula of the moisturizing cream is shown in the table 2-1.
TABLE 2-1
Representing no addition of the corresponding substance.
The preparation method comprises the following steps:
(1) and (3) putting the component A into an oil pan, and uniformly stirring and dispersing at 70 ℃ to obtain an oil phase.
(2) And (3) putting the raw material of the component B into an emulsifying pot, and dissolving and dispersing uniformly at 70 ℃.
(3) The oil phase was added to the water phase at constant speed with stirring, taking 2.0 minutes. After the addition was complete, the mixture was emulsified for a further 3.0 minutes at a homogenization speed of 4000 rpm. After the emulsification is finished, stirring is started at 400 rpm.
(4) Cooling to 40 deg.C, adding the component C into emulsifying pot, and stirring.
(5) Stirring, vacuumizing, cooling to 35 deg.C, and discharging.
Example 12
The difference from the example 11 is that the mass percent of the sebum bionic composition in the example 1 is 0.01 percent, the mass percent of the deionized water is increased correspondingly, but the mass percent is still increased to 100 percent, and the percentage content of the rest components is unchanged.
Example 13
The difference from the example 11 is that the mass percent of the sebum bionic composition in the example 1 is 30 percent, the mass percent of the deionized water is correspondingly reduced, but the mass percent is still increased to 100 percent, and the percentage content of the rest components is unchanged.
Example 14
The difference from the example 11 is that the mass percent of the sebum bionic composition in the example 1 is 0.008 percent, the mass percent of the deionized water is increased correspondingly, but the mass percent is still increased to 100 percent, and the percentage content of the rest components is unchanged.
Example 15
The difference from the example 11 is that the mass percent of the sebum bionic composition in the example 1 is 35%, the mass percent of the deionized water is increased correspondingly, but the mass percent is still increased to 100%, and the percentage content of the rest components is unchanged.
Example 16
An essence containing the sebum biomimetic composition disclosed by the invention has a formula shown in a table 2-2.
Tables 2 to 2
The preparation method comprises the following steps:
(1) and uniformly stirring and dispersing the component A at normal temperature to obtain an oil phase.
(2) And (3) putting the component B into an emulsifying pot, and dissolving and dispersing uniformly at 80 ℃.
(3) The oil phase was added to the water phase at constant speed with stirring, taking 2.0 minutes. After the addition was complete, the mixture was emulsified for a further 3.0 minutes at a homogenization speed of 4000 rpm. After the emulsification is finished, stirring is started at 400 rpm.
(4) Cooling to 40 deg.C, adding the component C into emulsifying pot, and stirring.
(5) Stirring, vacuumizing, cooling to 35 deg.C, and discharging.
Comparative example 11
A moisturizing cream not comprising the sebum biomimetic composition as described in the present invention, and the formula thereof is shown in table 3.
TABLE 3
The preparation method is the same as in example 11.
Comparative example 12
The difference from example 11 is that the sebum biomimetic composition of example 1 is replaced by equal mass percentage of jojoba oil of comparative example 1.
Comparative example 13
The difference from the example 11 is that the sebum bionic composition of the example 1 is replaced by the sebum bionic composition of the comparative example 6 with equal mass percentage.
Test example 1
Determination of radical scavenging ability of hyposuperoxide anion
A sample to be tested: sebum biomimetic compositions and one-component natural vegetable oils of examples 1-10 and comparative examples 1-10.
The specific determination method is as follows:
0.05mol/L of Tris-HCl buffer (4.5 mL) with pH 8.2 was preheated in a water bath at 25 ℃ for 20 min. Then adding 1mL of sample to be tested (namely the sebum bionic composition and the single-component natural vegetable oil provided in the examples and the comparative examples) and 0.4mL of 25mmol/L pyrogallol solution, mixing uniformly, reacting for 5min in a water bath at 25 ℃, and adding 1.0mL of 8mol/L hydrochloric acid to terminate the reaction. Absorbance values were measured at 299nm with Tris-HCl buffer as a reference.
Blank control experiment: only 1mL of ethanol was used in place of the test sample, and the other steps were not changed.
The clearance (D) was calculated according to equation (1).
D(％)＝[1-(A2/A1)]×100％ (1)
Wherein A1 is the absorbance value of the blank control; a2 is the absorbance value of the sample to be tested.
Test example 2
Measurement of hydroxyl radical scavenging ability
A sample to be tested: sebum biomimetic compositions and one-component natural vegetable oils of examples 1-10 and comparative examples 1-10.
The specific test method is as follows:
3mL of FeSO with the concentration of 2mmol/L are sequentially added into a 25mL colorimetric tube43mL of H with a concentration of 1mmol/L2O2Shaking, adding 3mL of salicylic acid 6mmol/L, shaking, heating in 37 deg.C water bath for 15min, and measuring absorbance; respectively adding samples to be tested (namely the sebum bionic composition and the single-component natural vegetable oil provided in the examples and the comparative examples) with certain concentration, shaking up, continuing to heat in water bath for 15min, and taking out to test the absorbance.
Blank control experiment: only 1mL of ethanol was used in place of the test sample, and the other steps were not changed.
The formula (2) is a formula for calculating the clearance rate of the sample to be tested to the carboxyl free radical (OH).
OH(％)＝(A3-A2)/A1×100％ (2)
In the formula, A1 is the absorbance value of the reaction system before adding the sample to be tested; a2 is the absorbance value of the system after OH is removed from the sample to be tested; a3 is the absorbance value of the system after blank control is cleared of OH.
The test results of test examples 1 and 2 are shown in table 4.
TABLE 4
As can be seen from Table 4, the sebum-mimicking compositions of examples 1 to 10 all had better radical-scavenging ability, while the effects of the examples were not achieved using either single-component natural vegetable oils (comparative examples 1 to 5) or compositions lacking one component (comparative examples 6 to 10).
Comparing examples 1-10, it can be seen that optimizing the formulation and selecting five natural vegetable oils in a specific ratio combination further improves the free radical scavenging ability of the composition.
Test example 3
Skin prick test
A sample to be tested: the sebum-stimulating composition, the single-component natural vegetable oil and the cosmetic provided in examples 1 to 16 and comparative examples 1 to 13 were each blended.
The specific test mode is as follows:
100 volunteers (50 women 50 men) were selected to evaluate the effect of the composition on resisting itch and red swelling of skin caused by histamine, and histamine hydrochloride (10mg/mL) was dropped on the skin test site with a test blade on the inner side of forearm of the subject, and after 5 minutes, the skin developed red swelling and itching. The test samples to be tested are respectively smeared, and the relieving effects of different samples on skin itch and red swelling caused by histamine resistance are compared.
Blank control: the only difference from the above test method is that nothing is coated, waiting for the skin to repair itself.
Evaluation method of skin redness and swelling degree: visual assessment of skin redness on 5 point scale, 0: non-red and non-swollen, 1: slight red swelling, 2: marked redness, 3 severe redness, 4 very severe redness; the average of 9 volunteers was calculated.
The test results are shown in tables 5 and 6.
TABLE 5
As can be seen from Table 5, the samples of examples 1 to 10 were excellent in the effect of relieving the red swelling and itching caused by histamine, as compared with the blank control and the comparative examples, in which the effect of example 1 was most remarkable, and the red swelling and itching condition was substantially eliminated 2.5 hours after application, while the effects of examples 1 to 10 could not be achieved by using one component of the natural vegetable oils (comparative examples 1 to 5) or the compositions lacking one component (comparative examples 6 to 10).
Comparing examples 1-10, it can be seen that optimizing the formulation and selecting five natural vegetable oils in a specific ratio combination can further enhance the efficacy of the composition in combating the red swelling and itching associated with histamine.
TABLE 6
| Time | 0h | 0.5 |
1h | 1.5 |
2h | 2.5h |
| Blank control | 4.05 | 3.81 | 3.52 | 3.3 | 2.62 | 2.45 |
| Comparative example 11 | 4.05 | 3.32 | 2.81 | 2.4 | 2.12 | 1.95 |
| Comparative example 12 | 4.01 | 3.36 | 2.74 | 2.54 | 2.23 | 1.85 |
| Comparative example 13 | 4.08 | 3.28 | 2.56 | 2.33 | 2.10 | 1.80 |
| Example 11 | 4.06 | 2.55 | 2.07 | 1.62 | 1.58 | 0.65 |
| Example 12 | 4.07 | 2.61 | 2.13 | 1.71 | 1.60 | 1.01 |
| Example 13 | 4.10 | 2.70 | 2.21 | 1.75 | 1.67 | 0.95 |
| Example 14 | 4.08 | 2.81 | 2.33 | 1.81 | 1.75 | 1.33 |
| Example 15 | 4.03 | 2.82 | 2.40 | 1.98 | 1.80 | 1.40 |
| Example 16 | 4.01 | 2.60 | 2.11 | 1.70 | 1.61 | 1.10 |
As can be seen from Table 6, the samples provided in examples 11-16 have obvious effects of relieving and repairing skin irritation and redness caused by histamine, particularly, the samples of example 11 have obvious effects of relieving skin redness and swelling stimulated by histamine after being applied to the samples for half an hour, the skin redness and itching are relieved, the discomfort basically disappears after 2.5 hours, and the blank control and comparative examples 11-13 have obviously poorer test results than the samples of examples 11-16 and still have redness and swelling and itching to a certain extent after 2.5 hours, so that the sebum biomimetic composition provided by the invention can provide good relieving and repairing effects for cosmetics after being added to the cosmetics.
It is understood from comparative examples 11 to 15 that the effect is better when the sebum-mimicking composition is added to the cosmetic in an amount of 0.01 to 30%, and that the effect is deteriorated when the amount is too large (example 15) or too small (example 14).
Fig. 1 is a graph comparing the improvement effect of the samples of example 11 and comparative example 11 and the blank sample against the symptom of skin itch caused by histamine, and it is apparent that the moisturizing cream of example 11 has better soothing and repairing effects.
Test example 4
Skin hydration test
A sample to be tested: the cosmetics of examples 11 to 16 and comparative examples 11 to 13.
The specific test method is as follows:
the moisture content of the skin is an important performance index of whether the barrier function of the skin is complete, the barrier function of the skin is damaged, the percutaneous moisture loss rate is increased, and the moisture content of the skin is reduced.
The subjects were 20 in total, 15 women and 5 men aged 20-40 years, with an average age of 28 years. Cleaning forearm of the testee, sitting at a set humidity for 30min, and selecting 3 × 3cm of forearm of the testee2The water content of each experimental part is detected by a Corneometer CM825 in a square experimental area by taking a left arm as a test area of a sample to be detected and a symmetrical area of a right arm as a blank control (nothing is coated), and the average value is obtained and recorded after 5 times of repetition. Skin waterThe calculation formula of the total rate is as follows:
the test results are shown in table 7.
TABLE 7
| Time | 0h | 0.5 |
1h | 2h | 3h | 4h |
| Example 11 | 0.56 | 83.31 | 73.45 | 64.34 | 61.50 | 52.35 |
| Example 12 | 0.61 | 82.87 | 74.22 | 62.13 | 60.32 | 52.21 |
| Example 13 | 0.63 | 83.71 | 75.98 | 61.52 | 59.31 | 51.98 |
| Example 14 | 0.70 | 82.31 | 71.36 | 59.47 | 55.63 | 48.32 |
| Example 15 | 0.68 | 82.13 | 72.48 | 60.21 | 54.17 | 49.37 |
| Example 16 | 0.59 | 82.01 | 74.31 | 65.36 | 60.50 | 51.98 |
| Comparative example 11 | 0.81 | 76.69 | 64.19 | 57.33 | 45.64 | 31.12 |
| Comparative example 12 | 0.78 | 77.65 | 65.58 | 58.33 | 46.10 | 35.32 |
| Comparative example 13 | 0.76 | 78.30 | 66.02 | 59.07 | 47.39 | 36.56 |
As can be seen from Table 7, the skin hydration rates of the subjects were still at a very high level, up to 52.35% and as low as 48.32% after 4 hours using the samples prepared in examples 11 to 16, while the skin hydration rates of the subjects were only up to 36.56% after 4 hours using the samples prepared in comparative examples 11 to 13, with a very significant difference, indicating that the compositions of the present invention have very good effects in protecting the barrier function of the skin when used in cosmetics, and that the skin moisture content was still at a high level after 4 hours of use, the transdermal moisture loss rate was greatly reduced, and the skin exhibited a tender, elastic, and shiny state.
Comparative examples 11 to 15 show that the effect is more excellent when the skin lipid biomimetic composition is added in the cosmetic in the range of 0.01 to 30%, and that the effect is deteriorated by too much (example 15) or too little (example 14).
Fig. 2 is a graph showing the change of skin hydration rate of the samples of example 11 and comparative example 11, after 4 hours, the skin hydration rate of the sample of example 11 is still at a very high level, reaching 52.5%, while the skin hydration rate of the sample prepared by comparative example 11 is only 31.12% after 4 hours, which is a big difference, thus proving that the cosmetic provided by the invention can play a better role in repairing the skin barrier, thereby slowing down the loss of water.
Test example 5
Patch test
A sample to be tested: compositions of examples 1 to 16 and comparative examples 1 to 13, single-component natural vegetable oils, and cosmetics.
The test method comprises the following steps: taking 60 females and 40 males as subjects, taking 0.03mL of sample by using an injector, placing the sample in a medicine chamber of a spot test adhesive tape, immediately and longitudinally sticking the spot test adhesive tape with the sample on normal skin of a left forearm from the lower part, and slightly pressing the medicine chamber one by one to expel air and uniformly distribute the test substances. The test site is marked for easy observation. Every 24 hours, and half an hour after the patch was removed, the examination was performed according to the terms of the CTFA guidelines (1981). The patch test was performed 6 times in total, and on day 6 after completion of the skin application, additional examination was performed to observe delayed reactions. The data is compared using the last examination. The identification criteria are shown in table 8. The test results are shown in table 9.
TABLE 8 identification standards
TABLE 9 test results of Patch experiments
As can be seen from table 9, the patch test results of the compositions, the single-component natural vegetable oil and the cosmetics in the examples and the comparative examples provided by the present invention are all 0 grade, i.e. no irritation and no erythema, which indicates that the components in the compositions and the cosmetics provided by the present invention are safe, mild and non-irritant.
It can be seen from the results of the above test examples 1 to 5 that the sebum biomimetic composition and the cosmetic containing the sebum biomimetic composition provided by the present invention have the outstanding effects of repairing skin barriers, moisturizing, soothing, anti-aging, restoring skin elasticity, etc., because the five natural vegetable oils in the sebum biomimetic composition provided by the present invention have synergistic effects, the sebum compositional structure of a human body can be simulated, a rawhide-imitated lipid layer can be formed on the skin surface, external damage can be effectively resisted, good effects of repairing skin barriers and promoting cell regeneration can be provided, and the sebum biomimetic composition is mild and non-irritant.
With the further optimization of the formula, specific components are selected and combined in a specific proportion, the sebum composition structure of a human body is simulated more accurately, and the capabilities of repairing skin barriers and promoting cell regeneration are further improved.
In addition, the sebum bionic composition is added into the cosmetics in an amount of 0.01-30%, and the obtained cosmetics can have good effects of repairing skin barriers and promoting cell regeneration, otherwise, adverse effects can be brought.
The present invention is illustrated in detail by the examples described above, but the present invention is not limited to the details described above, i.e., it is not intended that the present invention be implemented by relying on the details described above. It should be understood by those skilled in the art that any modification of the present invention, equivalent substitutions of the raw materials of the product of the present invention, addition of auxiliary components, selection of specific modes, etc., are within the scope and disclosure of the present invention.
Claims (7)
1. The sebum bionic composition is characterized by comprising the following components in parts by weight:
2. the sebum biomimetic composition according to claim 1, wherein the sebum biomimetic composition comprises the following components in parts by weight:
3. the sebum biomimetic composition according to claim 2, wherein the sebum biomimetic composition comprises the following components in parts by weight:
4. the sebum biomimetic composition according to claim 3, wherein the sebum biomimetic composition comprises the following components in parts by weight:
5. a cosmetic comprising the sebum-mimicking composition according to any one of claims 1 to 4;
the mass percentage of the sebum bionic composition in the cosmetic is 0.01-30%.
6. The cosmetic according to claim 5, wherein the cosmetic comprises any one of a moisturizing cream, a lotion, an essence oil and a mask patch.
7. The cosmetic according to claim 5, wherein the cosmetic is a moisturizing cream, and the mass percentage of the sebum biomimetic composition in the moisturizing cream is 0.01-30%.
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| CN110801412B (en) * | 2019-12-04 | 2023-04-07 | 广州睿森生物科技有限公司 | Sebum bionic composition, application thereof and cosmetics |
| CN111110596A (en) * | 2020-02-18 | 2020-05-08 | 广州栋方生物科技股份有限公司 | Sebum membrane bionic composition and preparation method thereof |
| AU2021227936A1 (en) * | 2020-02-28 | 2022-09-01 | Joyce H. Ma | Formulations and uses thereof |
| CN111557874B (en) * | 2020-04-29 | 2022-01-04 | 上海清轩生物科技有限公司 | Bionic skin barrier repairing composition containing camellia extract and application |
| CN112315819B (en) * | 2020-11-20 | 2022-09-02 | 烟台新时代健康产业日化有限公司 | Bionic skin lipid membrane compound and application thereof in soothing cosmetics |
| CN113041172B (en) * | 2021-03-22 | 2023-05-09 | 广州无添加主义化妆品有限公司 | Sebum membrane bionic composition for skin barrier repair, application thereof and cosmetics |
| CN112972311B (en) * | 2021-04-12 | 2022-06-21 | 杭州易蓬科技有限公司 | Anti-allergy and relieving composition containing sea buckthorn fruit oil |
| CN113559020B (en) * | 2021-08-18 | 2022-07-19 | 宝萃生物科技有限公司 | Composition for protecting and repairing skin barrier and preparation method and application thereof |
| CN114129476A (en) * | 2021-12-14 | 2022-03-04 | 上海家化联合股份有限公司 | Composition comprising vegetable fruit oil and vegetable seed oil and use thereof |
| CN114404326A (en) * | 2022-01-25 | 2022-04-29 | 媞颂日化用品(广州)有限公司 | Moisturizing composition for pet local care and preparation method and application thereof |
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Address after: 510663 No.15, Kelin Road, Guangzhou Science City, Guangzhou high tech Industrial Development Zone, Guangdong Province Patentee after: Guangzhou Huanya cosmetics technology Co.,Ltd. Address before: 510663 No.15, Kelin Road, Guangzhou Science City, Guangzhou high tech Industrial Development Zone, Guangdong Province Patentee before: GUANGZHOU HUANYA COSMETICS TECHNOLOGY Co.,Ltd. |