CN109844538A - 用于增强荧光的组合物和方法 - Google Patents

用于增强荧光的组合物和方法 Download PDF

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Publication number
CN109844538A
CN109844538A CN201780062491.4A CN201780062491A CN109844538A CN 109844538 A CN109844538 A CN 109844538A CN 201780062491 A CN201780062491 A CN 201780062491A CN 109844538 A CN109844538 A CN 109844538A
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China
Prior art keywords
alexa
dylight
antibody
fluorescent
spacer molecule
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Pending
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CN201780062491.4A
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English (en)
Chinese (zh)
Inventor
S·德塞
M·恩兰德
K·吉
M·贝克
R·阿吉勒
S·斯维尼
A·卢卡维什尼科夫
S-J·黄
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Pierce Biotechnology Inc
Life Technologies Corp
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Pierce Biotechnology Inc
Life Technologies Corp
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Publication of CN109844538A publication Critical patent/CN109844538A/zh
Pending legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/001—Preparation for luminescence or biological staining
    • A61K49/0013—Luminescence
    • A61K49/0015—Phosphorescence
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
    • A61K47/08—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing oxygen, e.g. ethers, acetals, ketones, quinones, aldehydes, peroxides
    • A61K47/10—Alcohols; Phenols; Salts thereof, e.g. glycerol; Polyethylene glycols [PEG]; Poloxamers; PEG/POE alkyl ethers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
    • A61K47/16—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing nitrogen, e.g. nitro-, nitroso-, azo-compounds, nitriles, cyanates
    • A61K47/18—Amines; Amides; Ureas; Quaternary ammonium compounds; Amino acids; Oligopeptides having up to five amino acids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/001—Preparation for luminescence or biological staining
    • A61K49/0013—Luminescence
    • A61K49/0017—Fluorescence in vivo
    • A61K49/005—Fluorescence in vivo characterised by the carrier molecule carrying the fluorescent agent
    • A61K49/0058—Antibodies
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Veterinary Medicine (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Inks, Pencil-Leads, Or Crayons (AREA)
  • Electroluminescent Light Sources (AREA)
CN201780062491.4A 2016-09-01 2017-09-01 用于增强荧光的组合物和方法 Pending CN109844538A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662382594P 2016-09-01 2016-09-01
US62/382,594 2016-09-01
PCT/US2017/049838 WO2018045278A1 (en) 2016-09-01 2017-09-01 Compositions and methods for enhanced fluorescence

Publications (1)

Publication Number Publication Date
CN109844538A true CN109844538A (zh) 2019-06-04

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CN201780062491.4A Pending CN109844538A (zh) 2016-09-01 2017-09-01 用于增强荧光的组合物和方法

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US (4) US20180092993A1 (enExample)
EP (2) EP3507603B1 (enExample)
JP (3) JP7216638B2 (enExample)
CN (1) CN109844538A (enExample)
ES (1) ES2979129T3 (enExample)
WO (1) WO2018045278A1 (enExample)

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CN113913404A (zh) * 2020-07-10 2022-01-11 上海米然生物科技有限公司 一种荧光交联RNase H突变体缀合物及其应用
WO2023088402A1 (zh) * 2021-11-22 2023-05-25 菲鹏生物股份有限公司 一种捕获法检测抗体的方法和应用

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US20220229047A1 (en) * 2019-06-20 2022-07-21 Konica Minolta, Inc. Linked body for fluorescent labeling agents
KR102718543B1 (ko) 2019-09-26 2024-10-18 소니그룹주식회사 링커 그룹을 갖는 중합체성 탠덤 염료
WO2021193682A1 (ja) * 2020-03-25 2021-09-30 積水メディカル株式会社 免疫学的分析方法及び免疫学的分析試薬キット
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WO2023032994A1 (ja) * 2021-08-31 2023-03-09 富士フイルム株式会社 化合物及びこれを用いた標識生体物質
CN115466960B (zh) * 2022-09-15 2023-05-26 河北工业大学 一种含苯并噻唑基团改性聚天冬氨酸荧光缓蚀剂的制备方法和应用
WO2025182762A1 (ja) * 2024-02-29 2025-09-04 富士レビオ株式会社 検出方法、並びに、これに用いる標識体及びキット
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CN113913404A (zh) * 2020-07-10 2022-01-11 上海米然生物科技有限公司 一种荧光交联RNase H突变体缀合物及其应用
WO2023088402A1 (zh) * 2021-11-22 2023-05-25 菲鹏生物股份有限公司 一种捕获法检测抗体的方法和应用

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EP3507603B1 (en) 2024-03-06
US11857643B2 (en) 2024-01-02
JP7216638B2 (ja) 2023-02-01
JP2025028883A (ja) 2025-03-05
ES2979129T3 (es) 2024-09-24
US20200330610A1 (en) 2020-10-22
US11865191B2 (en) 2024-01-09
EP3507603A1 (en) 2019-07-10
US20240042062A1 (en) 2024-02-08
EP4425179A3 (en) 2024-12-11
EP4425179A2 (en) 2024-09-04
US20200261602A1 (en) 2020-08-20
WO2018045278A1 (en) 2018-03-08
JP2023025018A (ja) 2023-02-21
JP2019531474A (ja) 2019-10-31
US20180092993A1 (en) 2018-04-05
EP3507603C0 (en) 2024-03-06

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