CN1098066C - Body cosmetic pigment composition and its producing method - Google Patents

Abstract

The present invention is related to a body cosmetic pigment composition used for enhancing elasticity of the skin and helping losing a weight and its method, in which the body cosmetic pigment composition includes various ingredients, such as calcium, potassium, iodine, selenium, alginic acid, and the like, and viscous solutions, such as inorganic silica, aluminum and magnesium, obtained from the loess.

Description

Body cosmetic pigment composition and preparation method thereof

The present invention relates to a kind of cosmetic pigment composition and preparation method thereof, relate in particular to a kind of skin elasticity and slimming body cosmetic pigment composition that is used to improve health.

Disclose the compositions that a kind of physical beauty of being used for perhaps treats among the French Patent (FRP) FR2489689, it comprises clay, plant, cryodesiccated sea water and cryodesiccated Sargassum.Said composition preferably includes 1-3 (particularly 2-3) kilogram clay, 30-80 (particularly 80) gram plant, 10-50 (particularly 35) the cryodesiccated sea water of gram and 10-40 (particularly 30) and restrains cryodesiccated Sargassum.Said composition can directly be added in the body lotion, or plant added in the body lotion (preferably adds with the part clay), and the compositions of other component is coated on the health before bathing.Described compositions can be used as the bathing additive, plays to regulate circulate, loosen, slender and graceful in stature, prevent eczema or analgesic effect.

Along with civilization progress, people absorb meat more than vegetable because the living condition takes a turn for the better, and usually do not get enough athletic exercise owing to traffic convenience again, and therefore fat number heightens.But obesity is the main root of various diseases.Particularly the skin of abdominal part and waist is healthy index, and stature is seriously influenced.For above-mentioned reasons, needing a kind of cosmetic composition to improve skin elasticity and help loses weight.

The objective of the invention is a kind of body cosmetic pigment composition that satisfies above-mentioned requirements and preparation method thereof.

An object of the present invention is to provide a kind of body cosmetic pigment composition, it contains the various compositions that extract, the sodium chloride solution of purifying and the various inorganic constituents that obtains from loess from sea water from sea-plant.

Another object of the present invention provides a kind of body cosmetic pigment composition, wherein sea-plant is brown seaweed and Thallus Laminariae (Thallus Eckloniae), the component of extracting from sea-plant comprises calcium, potassium, iodine, selenium and alginic acid, and loess is one of Kaolin and montorillonite clay, and inorganic component is the various viscosity solutions that contain silicon dioxide, aluminum and magnesium.

Another object of the present invention provides a kind of method for preparing body cosmetic pigment composition, and it comprises the step of extracting various components and carrying out a series of processing from sea-plant; The step of from loess, extracting various inorganic components and carrying out various processing; With the step of mixing and stir the pure solution that from sea-plant composition extraction step and inorganic constituents extraction step, extracts.

Other purpose of the present invention provides a kind of method that is used to produce body cosmetic pigment composition, wherein the extraction step of sea-plant component is to carry out continuously: the 1st step, operation below being used to repeat for several times, promptly in refrigerator with brown seaweed and the freezing preset time of Thallus Laminariae (Thallus Eckloniae), in thawing apparatus, melt preset time again; The 2nd step is used for from the sea water sodium chloride solution of purifying; The 3rd step, the sodium solution that is used for purifying joins the brown seaweed and the Thallus Laminariae (Thallus Eckloniae) of the 1st step, adopts disintegrating apparatus that they are pulverized again; The 4th step is used for by adopting filter to tell the solution that solid particle extracts brown seaweed and Thallus Laminariae (Thallus Eckloniae) from the comminuted material of the 3rd step; The 5th step, be used for adding organic acid this solution of purifying by solution toward the brown seaweed of the 4th step and Thallus Laminariae (Thallus Eckloniae), wherein the 1st step repeats following operation 1～5 time, i.e. freezing brown seaweed and Thallus Laminariae (Thallus Eckloniae) 48 hours in-7 ℃～+ 3 ℃ refrigerator, in 1 ℃～5 ℃ thawing equipment, melted 6 hours again, wherein the inorganic constituents extraction step carries out continuously by the 6th step, to extract stickum from loess, as silicon dioxide, aluminum and magnesium; The 7th step is used for from the sea water sodium chloride solution of purifying; The 8th step is used for various compositions and sodium chloride solution mixing and stirring with the 6th and the 7th step; The 9th step is used for depositing precipitum from the mixture of the 8th step, then tells the precipitum that is deposited in the bottom; With the 10th step, be used for extracting pure solution from the mixture of the 9th step.

Fig. 1 represents to be used to produce the device of the extraction sodium chloride of cosmetic pigment of the present invention;

Fig. 2 is the enlarged perspective of the circular defecator of rotation of the extraction sodium chloride device of presentation graphs 1; With

Fig. 3 is the technological process of the method for expression preparation body cosmetic pigment composition of the present invention.

Body cosmetic pigment composition of the present invention be by with various from sea-plant, the predetermined process preparation after mixing such as the composition and the sodium chloride solution that extract in sea-tangle and the brown seaweed, silica, aluminium, magnesium etc. The feature of brown seaweed and sea-tangle is at first described and the method for its solution that is used for purifying.

Brown seaweed and sea-tangle contain abundant calcium and potassium, and especially, potassium is the twice of sodium. When taking in a large amount of potassium, potassium has so just prevented that water from entering body interior in a large number basically along with the sodium excretion in the urine.

In addition, brown seaweed and sea-tangle contain abundant iodine. Iodine and thyroid gland have close relationship, so iodine deficiency may cause thyroid disease. Particularly well-known hormone secretion causes weightening finish unusually, and thyroid function is destroyed and can be prevented by brown seaweed and the sea-tangle of taking in the marine alga.

Brown seaweed and sea-tangle also contain selenium. Selenium is present in seawater and the soil with considerably less amount, and is the necessary Rare Earth Mine material of human body metabolism. And selenium is the component of the sweet phthalein peroxidase of paddy Guang that prevents the oxidized or division of cell. Therefore, selenium is considered to the necessary mineral matter of human body cell function.

Particularly brown seaweed and sea-tangle contain a large amount of alginic acid. Contain various antibiotic, physiological agents and various mineral matter in the known alginic acid by testing, and when with alginic acid injection testing mouse, natural lipid and cholesterol reduce.

The component analysis table of [table 1] brown seaweed and Thallus Laminariae (Thallus Eckloniae)

			Brown seaweed (live body)	Thallus Laminariae (Thallus Eckloniae) (live body)
					Water (%)			88.8	91.0
Protein (%)			2.1	1.1
					Lipoidis (%)			0.2	0.2
Carbohydrate (gram)		Saccharide	4.4	3.6
							Cellulose	0.6	0.6
Pulverized limestone (gram)			3.9	3.5
					Inorganic matter (milligram)		Calcium	153	103
		Phosphorus	40	23
							Ferrum	1.0	2.4
		Receive	-	554
							Potassium	-	1242
Vitamin	A	Vitamin A (R, E)	08	129
							Xanthoplane (microgram)	0	0
		Beta-carotene (microgram)	1854	774

	B	Thiamine (milligram)	0.06	0.03
							Riboflavin (milligram)	0.16	0.13
	Niacin usp nicotinic acid (milligram)		1.0	1.1
						Ascorbic acid		18	14

When eating brown seaweed and Thallus Laminariae (Thallus Eckloniae), they are ground by tooth, are dissolved by digestive enzyme, and are absorbed in the internal organs with ionic condition.Therefore, the various compositions of brown seaweed and Thallus Laminariae (Thallus Eckloniae) extract with ionic condition and not need the plain mode of many steps be important by skin absorbs in capillary tube with aforesaid.

Therefore, the ion that makes various compositions enters from brown seaweed and Thallus Laminariae (Thallus Eckloniae) that to extract solution be primary and foremost purpose of the present invention.But, be heated to about 100 ℃ and extract meeting its physiologically active of destruction and some mineral.

In the present invention, the solution of brown seaweed and Thallus Laminariae (Thallus Eckloniae) extracts by they are freezing repeatedly and thawing, and this is owing to destroying the somatic tissue of leaf like this, so that extract various components at an easy rate.

When repeat-7 ℃ freezing then again when melting for+5 ℃, leaf is the easiest to be destroyed.On the contrary, when repeat-3 ℃ freezing and when+1 ℃ melts, the destructiveness of cell tissue is reduced to unacceptable degree.Can think that the leaf histiocyte can destroy by reaching cold point.So, leaf is to adopt grater, and for example ' Polytron pt2000 ' grinds with the speed that per minute 6000 changes, to destroy the leaf greater than 90%.When grinding, only be that leaf grinds be difficult.Therefore, in order to grind leaf effectively, sodium chloride solution is mixed with leaf until suitable concentration is arranged in leaf, the concentration of sodium chloride solution is about 2.0～2.2% like this.On the other hand, carry out live body brown seaweed and Thallus Laminariae (Thallus Eckloniae) freezing, to store or handle all be effective.

Secondly, the effect of explained later sodium chloride.

As everyone knows, human body mainly is to be made of water.Water dissolution is multiple to be physiological reaction intermediate product in the nutrient of biological active substances and mineral and the cell.

The total amount of water is about 60～70% of body wt in the human body, and the man generally has higher percentage ratio than the woman, and fat people is more than the thin body's.Water in the human body is meant body fluid, and it can be divided into two classes, intracellular fluid and extracellular body fluid.

Intracellular fluid is the water that is present in the cell, accounts for 2/3 of body fluid.Remaining 1/3 body fluid is to be dispersed in the extracellular body fluid.Therefore, for the adult of 70 kg body weight, the total amount of body fluid is about 40 liters, and wherein about 25 liters is intracellular fluid, and about 15 liters is extracellular body fluid.On the other hand, extracellular body fluid is divided into blood plasma and intracellular fluid, and its ratio is 1: 3.

The water that people take in every day mainly enters in the body by the oral cavity by drinking water or eating the food that contains water.In addition, it also can produce as metabolic by-product in health, although amount seldom.Usually, there was 2500 milliliters water to enter human body approximately in one day, accounts for its 90% about 2200 milliliters simultaneously and take in by the oral cavity.On the other hand, even the water yield that loses from human body changes according to ambient temperature and movement degree, under general condition, the aqueous dispersion of about 900 milliliters of absorptions is drained in health and by the evaporation of respiratory apparatus and skin, and wherein people sensuously can not feel to dry out.

The principal element of the distribution of definite water in human body is an osmotic pressure.In general, water is to move to hypertonic solution from hypotonic solution, osmosis that Here it is.On one side be that sugar juice and another side are between the water time when semipermeable membrane is placed on for example, sugar juice can not pass this film, moves to sugar juice but water can pass this film.As mentioned above, when adopting semipermeable membrane to separate solution and solvent, just produce osmosis.At this moment, this solution has special pressure.This pressure is called osmotic pressure.When temperature maintenance one timing, osmotic pressure is directly proportional with the concentration of solution.Solution with phase isosmoticity is called as isosmotic solution, and the solution that has than Hyposmolality is called as hypisotonic solution.Osmotic pressure is in physiological phenomenon, as playing an important role owing to osmotic pressure produces in edema and the erythrocytic haemolysis.

Osmotic pressure is meant the numbers of particles that is dissolved in the unit volume solution.When controlling the osmotic pressure of body fluid by the change numbers of particles or the water yield, human body is controlled the osmotic pressure of body fluid by the water yield in the change health.This control action is controlled by thirsty or anti-urine hormone.

Because water moves by freely spreading between cell, extracellular body fluid and intracellular fluid have equal osmotic pressure.If the osmotic pressure of any body fluid changes, the osmotic pressure that water can spread until two kinds of body fluid again equates.

Determine the concentration that principal element is a sodium chloride of osmotic pressure,effective, 90% of whole extracellulars body fluid of its formation generation osmotic pressure,effective.Therefore, the concentration of raising and reduction sodium chloride is accompanied by the change of osmotic pressure and cell volume.

The water of taking in as the people is during more than the water that drains, and health is in the excessive state of water.Follow excessive water and become extracellular body fluid to increase cell volume and dilution solute.Therefore, hydrone continues to move to from extracellular body fluid that the osmotic pressure until two kinds of body fluid is equal to each other the intracellular fluid.As a result, more aqueous dispersion is come out in cell rather than from cell.The water redispersion phenomenon of the total body fluid volume that Here it is by changing health.

In addition, when the people took in the sodium chloride solution of high concentration, the concentration of sodium chloride in the body fluid of extracellular raise.Simultaneously, although more sodium chloride enters cell, the drainage rate of sodium chloride also improves, and the raising major limitation that makes salt is in extracellular body fluid part.Therefore, removing water when the osmotic pressure of two kinds of body fluid is equal to each other, promoted water from the diffusion of intracellular fluid to extracellular body fluid.

Between blood plasma and intracellular fluid, the power and water in the blood plasma is separated matter and is discharged the such blood vessel of blood capillary by the colloid osmotic pressure of plasma proteins.The import of body fluid and outlet are to come balance by the pressure that is used for oppositely extracting out by the colloid osmotic pressure of plasma proteins the intracellular fluid of blood vessel.Osmotic pressure principle that Here it is.Adopt above-mentioned principle to stop water excessive mobile by the sodium chloride that in blood plasma, keeps normal amount.When the solution that will contain sodium chloride was used for sagging skin, the concentration of intracellular fluid entered blood capillary by sodium chloride and raises immediately.Then, the concentration of rising makes the water in the cell be shifted out from transit cell, and the result gets rid of external by the water that kidney will be collected in the cell.

The 3rd, the following describes the ion exchange that is used to guarantee stickum.Stickum only is meant the stickum that extracts from loess.Stickum has the beat all effect of the skin of making rubber-like.The stickum of this loess is owing to they obtain in conjunction with being difficult to ionic bond.But when adopting sea water, they are easy to obtain by ion exchange.Stickum is the second kind of material of mineral that contains such as silicon dioxide, aluminum, magnesium etc.

Loess generally is meant a part that is xanchromatic soil.But the color of soil changes with composition contained in the soil.Soil is attached in the production method usually again, and changes its form according to environmental condition.This soil generally is divided into five classes, for example Kaolin, montorillonite clay, Illite, chlorite, Vermiculitum.The wherein ratio of silicon dioxide and aluminum is depended in its classification.

The experiment of soil stickiness feature shows that Kaolin and montorillonite clay have outstanding quasi-viscous effect.Particularly it show when stickum is on being used in skin produce very strong tight.Tight degree is montorillonite clay＞Kaolin＞chlorite＞Illite＞Vermiculitum.Therefore, according to experimental result, can adopt Kaolin and montorillonite clay effectively as stickum.

For the stickum that obtains being suitable for, that natural loess is levigate.Their twice or three times, then are dissolved in them in the pure water to select little part fully by a sieve.Simultaneously, after telling not the dissolving part, the loess of stirring and dissolving and and sea water mixing, keep this mixture to precipitate again, from wherein separating stickum until loess.Then separate loess only to obtain stickum.The stickum that obtains is mixed with the composition that extracts from brown seaweed and Thallus Laminariae (Thallus Eckloniae).Then, this stickum that will be obtained by the ionic replacement thing combines with this composition electricity with the sea-plant of ionic condition in sea water, thereby obtains being combined with the cosmetic pigment of stickum.

The 1st embodiment

The extraction component of several brown seaweeds and Thallus Laminariae (Thallus Eckloniae)

I) raw material

Adopt the coarse raw materials of 1 kilogram of brown seaweed and Thallus Laminariae (Thallus Eckloniae).

Ii) deodorization

Rotate raw material and in 60 minutes, come deodorization with inherent abnormal smells from the patient in removal brown seaweed and the Thallus Laminariae (Thallus Eckloniae) by the vibrating device that adopts 500～1000RPM with the current washing.

Iii) leaf disorganization

Undertaken wherein will treating that by triplicate deodorization material freezing 48 hours and step of melting 6 hours continuously in-5 ℃ refrigerator produce freezing point and destroy about 50% leaf histiocyte in 3 ℃ melter, so that several component can be extracted.

Iv) add sodium chloride solution and grind the mixture of leaf tissue and sodium chloride solution

Toward the sodium chloride solution (2.0～2.2%) that wherein adds 800 milliliters, adopt grinder (Polytron pt2000) they to be ground at 6000RPM, pulverize about 90% leaf tissue, to extract several components.

V) filter

By separating with two-layer filter screen and filtering ground material and extract required solution. The 2nd embodiment

The purification of sodium chloride solution (2.0～2.2%)

I) introduce sea water

Sea water is extracted in the storage tank on ground, makes its concentration adopt salinity to measure instrumentation and be decided to be 2.0～2.2%.

The sea water of ii) purifying

The aforesaid purifier of preferred employing prepares pure sodium chloride solution by the impurity that separates in the sea water, as depicted in figs. 1 and 2.

Purifier be by the circular defecator 3 of rotation that is used for storing the storage tank 1 that will use sea water, link to each other with storage tank 1 by pipeline 2, the sea water that is used for joining the circular defecator 3 of rotation with impurity isolating and store it storage tank 4, be used for from the pump 5 of last storage tank 5 delivered solution and the steaming and decocting filter 6 that is used for the solution that last filtration squeezes into by the pump pressure of pump 5 constitutes.In addition, the pipeline 2 that links to each other with the circular defecator 3 of rotation that is provided with blowing motor 7 prevents that to be used to carry air sea water is by germ contamination.Particularly, be arranged on the filter paper 8 that rotates in the circular filter 3 at rotating shaft 9 places, so that filtering impurity spatially separates with sea water.

According to said method, pure sodium chloride solution is being collected in the steaming and decocting filter 6 through after the series of steps.The pure sodium chloride solution that is collected in the steaming and decocting filter 6 can be used for producing body cosmetic pigment composition. The 3rd embodiment

From silicon dioxide, aluminum and magnesium solution, extract stickum

Levigate loess with twice or three times of sieve screening,, is dissolved in them in the water of purification as the little part of sand with selection, discards undissolved part, selects dissolved part, then in the dark dry 1 hour.Separate the water of purifying, make loess be deposited in the bottom, they are mixed with sodium chloride solution (1000 milliliters), stirred 3～4 hours, in the dark with their dry one day, then separate and subside is at the loess of bottom, add NaOH solution in the mixture of step process in front and be about 7.0 until mixture pH, produce coagulation, they are purified, obtain the stickum of ionic condition suddenly. The 4th embodiment

Makeup water

By uniform mixing and stir the listed component preparation makeup water of table 2 below all.

[table 2]

Component	Embodiment 4	The comparative example 1	The comparative example 2	The comparative example 3
					1. sodium chloride solution	Weight (reaching 100)	100	100	-
2. the cosmetic pigment of the 1st embodiment	30	-	30	30
					3. the cosmetic pigment of the 3rd embodiment	20	20	-	20
4. propyl parabene	0.1	0.1	0.1	0.1
					5. coloring agent	In right amount	In right amount	In right amount	In right amount
6. blended spice	0.1	0.1	0.1	0.1

Therefore, being used for improving skin elasticity and slimming body cosmetic pigment composition contains from the wherein a kind of of brown seaweed and Thallus Laminariae (Thallus Eckloniae) or various components that the two extracts, for example calcium, potassium, iodine, selenium, alginic acid etc., with the viscosity solution that from loess, obtains, for example inorganic silicon dioxide, aluminum and magnesium.

As shown in Figure 3, the method that is used to produce body cosmetic pigment composition comprises: the 11st step 12, be used for mixing pure solution from sea-plant component extraction step 10 and 11 extractions of inorganic constituents extraction step, wherein step 10 refers to that extracting various compositions from sea-plant carries out series of processing steps then, step 11 is extracted inorganic component for being used for from loess, for example silicon dioxide, aluminum and magnesium, and pass through series of processing steps; The 12nd step 13, mixture by the 11st step 12 is used to purify; The 13rd step 14 is used for the product solution by the 12nd step is joined each bottle; And the 14th step 15, being used for will be by the bottled case of the 13rd step 14.Sea-plant composition extraction step 10 comprises: the 1st step 16 is used for extracting various components from brown seaweed and Thallus Laminariae (Thallus Eckloniae); The 2nd step 17 is used for from the sea water sodium chloride solution of purifying; The 3rd step 18 is used to mix and grinds the 1st and the 2nd step 16,17 various compositions and sodium chloride solution; The 4th step 19 is used for adopting the mixture separate solid particles of two-layer filter from the 3rd step 18; With the 5th step 20, be used for extracting pure solution by the mixture that organic acid is joined the 4th step 19.Inorganic component extraction step 11 comprises: the 6th step 21 is used for extracting stickum from loess, for example silicon dioxide, aluminum and magnesium; The 7th step 22 is used for from the sea water sodium chloride solution of purifying; The 8th step 22 is used to mix and stirs the 6th and the 7th step 21,22 various components and sodium chloride solution; The 9th step 24 is used for the mixture deposition sedimentation thing from the 8th step 23; With the 10th step 25, be used for extracting pure solution from the mixture of the 9th step 24.

Specifically, the 1st step 16 is carried out the operation below to five time: freezing brown seaweed and Thallus Laminariae (Thallus Eckloniae) 48 hours in the refrigerator in-7 ℃ to+3 ℃ scope, and in 1 ℃～5 ℃ scope, in thawing apparatus, melt again and extracted various components in 6 hours. The susceptiveness of using and the contrast experiment of safety

Product according to the 4th embodiment and comparative example's 1～3 generation experimentizes to 20 people.Each fixed point in them is 1 to 5, and these points are average.Point 5 is meant very good, and point 4 is meant good, and point 3 is meant normal, and it is poor that point 2 is meant, point 1 is meant non-constant.Experimental result is described below.

It is 4.5 points that the 4th embodiment represents to use susceptiveness, and persistency is 4.2 points, and safety is 4.6 points;

It is 4.0 points that the 1st comparative example represents to use susceptiveness, and persistency is 3.9 points, and safety is 4.5 points;

It is 4.1 points that the 2nd comparative example represents to use susceptiveness, and persistency is 4.0 points, and safety is 4.0 points;

It is 3.9 points that the 3rd comparative example represents to use susceptiveness, and persistency is 3.9 points, and safety is 4.0 points;

By the research The above results, cosmetic composition of the present invention has good stability in use and safety.

As mentioned above, cosmetic pigment composition of the present invention makes the skin of excessive sag have unisexuality, and has slimming effect, so that health has pliability.In addition, this cosmetics provide inorganic matter and mineral etc. to help to prevent skin aging by the blood capillary that links to each other with skin to Skin Cell, and further produce the effect that keeps physical aptitude.

Claims (5)

1. body cosmetic pigment composition, comprise the component of extracting from sea-plant, sodium chloride solution of from sea water, purifying and the inorganic constituents that obtains from loess, wherein sea-plant is brown seaweed and Thallus Laminariae (Thallus Eckloniae), the component of extracting from sea-plant is calcium, potassium, iodine, selenium and alginic acid, loess is one of Kaolin and montorillonite clay, and inorganic component is the viscosity solution that contains silicon dioxide, aluminum and magnesium.

2. method for preparing body cosmetic pigment composition comprises: extract various compositions and carry out the step of a series of treatment process from sea-plant; Extract various inorganic components and carry out the step of a series of treatment process from loess; With the step of mixing and stir the pure solution that extracts from sea-plant component extraction step and inorganic component extraction step.

3. the preparation method of the body cosmetic pigment composition of claim 4, wherein the extracting method of sea-plant component carries out through the following steps continuously: the 1st step is used for repeating carrying out several times in the freezing brown seaweed of refrigerator and Thallus Laminariae (Thallus Eckloniae) preset time and the operation of melting preset time in thawing apparatus; The 2nd step is used for from the sea water sodium chloride solution of purifying; The 3rd step is used for adding the sodium chloride solution of purifying toward the brown seaweed and the Thallus Laminariae (Thallus Eckloniae) of the 1st step, adopts reducing mechanism that they are pulverized again; The 4th step is used for by adopting filter to extract the solution of brown seaweed and Thallus Laminariae (Thallus Eckloniae) from the comminuted material separate solid particles of the 3rd step; With the 5th step, be used for adding organic acid and extract pure solution by solution toward the 4th brown seaweed and Thallus Laminariae (Thallus Eckloniae).

4. the preparation method of the body cosmetic pigment composition of claim 5, wherein the 1st step is the operation that repeats freezing brown seaweed and Thallus Laminariae (Thallus Eckloniae) 48 hours in 1～5 refrigerator in-7 ℃～+ 3 ℃ scopes and melt 6 hours in 1 ℃～5 ℃ thawing apparatus.

5. the preparation method of the body cosmetic pigment composition of claim 4, wherein the extraction step of inorganic component carries out through the following steps continuously: the 6th step is used for extracting stickum from loess, as silicon dioxide, aluminum and magnesium; The 7th step is used for from the sea water sodium chloride solution of purifying; The 8th step is used for various compositions and sodium chloride solution mixing and stirring with the 6th and the 7th step; The 9th step is used for depositing precipitum from the mixture of the 8th step, then discards the precipitum that is deposited in the bottom; With the 10th step, be used for from the mixture of the 9th step solution of purifying.