RU2489934C2 - Method for production of biologically active products of brown algae and fruits - Google Patents

Abstract

FIELD: food industry.

SUBSTANCE: method envisages raw materials handling whereat one performs brown algae blades taking, rhizoids cutting-off, washing and drying. Dried algae are washed in fresh water and demineralised. The solution wherein demineralisation is performed is filtered, desalinated and separated. The algae are washed to remove acid in fresh water, soaked in fresh water; then water is drained. One mills the washed algae and adds strained fruits of the following group: cranberries, dried plums, dried apricots, red bilberries, sea-buckthorn berries, rosehips and holy thistle and places the mixture into the cooking vessel. Into the said vessel one adds at least a part of the filtered and desalinated solution wherein demineralisation was performed, drinking water and at least one food alkali. One performs cooking with continuous stirring, then - the mixture cooling and homogenisation. Then one introduces solutions of citric acid and calcium food salts, thoroughly stirs, proceeds with heating and conversion into a form suitable for storage.

EFFECT: invention ensures production of a product in the form of a stable complex with high content of biologically active digestible substances.

2 tbl, 4 ex

Description

The invention relates to the production of the food product “Brown homogenized algae (kelp and fucus) for dietary and therapeutic) nutrition” produced from varieties of kelp (seaweed) and / or fucus with the addition of various fruits containing biologically active substances.

Laminaria is one of the most common representatives of brown algae. It lives in the Far Eastern and Northern seas. The algae thallus attaches to the substrate with the help of rhizoids. A cylindrical stem (up to 50 cm long) departs from the rhizoids, it passes into a solid or dissected plate 4-5 m long. Fucus is another representative of brown algae. It is a branched bush attached to a substrate by a disk (rhizoid). The branches are belt-shaped, on some there are air-filled cavities filled with air and serving to maintain the plant in an upright position.

Brown algae contain substances necessary for the human body and are used in the preparation of various biologically active preparations, however, the composition of substances contained in brown algae is not wide enough, due to the comparative narrowness of the activity ranges, and their organoleptic properties are unusual for most consumers.

In addition, the products prepared according to the proposed method contain one or more fruits, which allows you to expand the composition of the substances contained in the product, expand the range of activity and make the organoleptic properties acceptable to most consumers.

The composition of the drug contains the fruits listed below.

Cranberries (Oxycoccus), a genus of plants of the lingonberry family, often included in the genus Vaccinium. Cranberries contain 4-5% sugars (mainly glucose and fructose). From organic acids, malic, citric and benzoic. Benzoic acid is a natural preservative. Pectin and tannins are also contained, as well as many macro- and microelements. Cranberries increase the secretion of the glands of the gastrointestinal tract. The proanthociapidn contained in cranberries does not allow pathogenic bacteria to linger in the body. Fresh cranberries and its juice can be used to prevent oncological diseases and diseases of the heart and blood vessels, with urinary tract infections, sore throat, colds. Ursolic acid, which has a hormone-like (corticoid) effect, is obtained from cranberries and helps to expand the coronary vessels of the heart.

Prunes contain 9-17% sugars - fructose, glucose, sucrose, organic acids - malic, citric, oxalic and slightly salicylic, pectin; tannins and nitrogenous substances; vitamins A, C, B1, B2, P; minerals - a large amount of potassium and phosphorus, less sodium, calcium, magnesium and iron. It is useful for vitamin deficiency and anemia (especially iron deficiency), due to the abundance of ballast substances, prunes are necessary for the gastrointestinal tract. Prunes have good antibacterial properties and are not inferior to medicines in terms of effectiveness: they slow down growth and even have harmful effects on bacteria that cause diseases of the oral cavity. Prunes are much more effective than any other natural remedies in the early stages of cardiovascular disease. Prunes contribute to the normalization of pressure, normalize the metabolism in the body and the gastrointestinal tract. Prunes are one and a half times more potassium, which is necessary for "setting" the kidneys, than in bananas, which are considered the best "healers" of urolithiasis. Antioxidants contained in prunes increase immunity and body resistance in environmentally unfavorable conditions. Prunes absorb free radicals that destroy the body: an excellent tool for the prevention of cancer and youth.

Dried apricots contain mineral salts, in particular potassium and calcium. From medical research it is known that a lack of potassium leads to a violation of cardiac activity and conduction of the nerve pathways. In addition, potassium increases the tone of the heart muscle and helps to eliminate excess sodium and fluid from the body. It is believed that the daily human need for potassium is about three grams, that is, it is enough to eat 150 grams of dried apricots. It has a lot of iron, as well as vitamins - C, B1, B2, PP, carotene (provitamin A). Thanks to the chemical composition of dried apricots, it also has a beneficial effect on the hematopoiesis process, helps strengthen immunity, and removes bad cholesterol. The dietary properties of dried apricots are mainly due to the significant predominance of potassium salts over sodium salts. The decongestant, diuretic effect of thick infusions and decoctions of dried apricots without sugar has been known for a long time. They are widely used in the treatment of many diseases of the cardiovascular system and kidneys. The relatively coarse fiber of dried apricots after soaking and, moreover, boiling, not lingering for a long time in the stomach, moderately excites intestinal motility.

Lingonberries are rich in dietary fiber and anthocyanins. Lingonberry has a relatively large amount of vitamin C (15 mg); it has provitamin A, potassium, calcium, magnesium, phosphorus and iron. Due to the presence of benzoic acid in the fruits of lingonberry, this berry is well preserved in air and in water. Lingonberry contains carbohydrates, useful organic acids (citric, salicylic, malic, etc.), pectin, carotene, tannins, vitamins A, C, E. Lingonberry berries contain up to 10-15% sugars (glucose, sucrose, fructose ) In lingonberry berries there are many minerals necessary for our body: potassium, calcium, magnesium, manganese, iron and phosphorus. Due to the large amount of benzoic acid, lingonberry berries are well preserved and have preservative properties. With rheumatism, lingonberry hypertension has an anti-sclerotic effect, strengthens the walls of blood vessels. Lingonberry berries are used for pulmonary tuberculosis, gastric catarrh, kidney stones, as a vitamin and antiseptic agent. Lingonberry juice sharpens vision, and is very useful for neurosis and anemia.

Sea buckthorn contains 2.8-7.8% fatty oil, ascorbic acid (vitamin C), carotene (provitamin A), vitamins B1, B3, carotenoids; riboflavin, tocopherol, lycopene, folic acid, phylloquinone, sugars, tannins, oleic, stearic, lyoleic and palmetic acids. Sea buckthorn fruits are the best natural vitamin carriers. The sea buckthorn bark contains serotonin (alkaloid hippofaine), which is tested in numerous clinics as an antitumor agent. Sea buckthorn is prescribed inside for peptic ulcer of the esophagus and stomach, various vitamin deficiencies. For skin diseases, long non-healing ulcers and wounds, in gynecological practice, for radiation damage to the skin and mucous membranes, externally aqueous paste and sea buckthorn oil are used. Compresses from sea buckthorn leaves are used for rheumatism. Sea buckthorn is an excellent multivitamin remedy for the prevention of strokes and heart attacks. Sea buckthorn oil is prescribed in the treatment of burns, frostbite, poorly healing external ulcers, eczema, peptic ulcer and duodenal ulcer, as a prophylactic to reduce degenerative processes of changes in the mucous membrane of the esophagus, used to treat diseases of the cardiovascular system, used in cases of intoxication, help with some blood diseases.

Rose hips are a "valuable multivitamin concentrate." They contain B vitamins, carotene, ascorbic acid, vitamins P, K, E. Rosehips have 10 times more vitamin C than black currants, 50 times more than lemon, 100 times more than apples. By the amount of carotene, rosehip successfully competes with sea buckthorn, apricot, persimmon, carrots, and orange. For therapeutic and prophylactic purposes, a decoction of fruits is widely used: it increases the body's resistance to colds and infectious diseases, helps to restore working capacity with mental and physical overwork. A decoction of fruits strengthens the walls of blood vessels, helps with a number of diseases of the liver, kidneys, bladder, with cholelithiasis, headache. The infusion slows the development of atherosclerosis, stimulates the function of the gonads, weakens and stops bleeding, reduces the permeability and fragility of blood vessels, helps with burns and frostbite. Rosehip oil stimulates the regeneration of damaged skin tissues and mucous membranes.

The main constituent of the medicinal plant of milk thistle is a rare biologically active substance - silymarin, in addition, microelements such as zinc, copper, selenium, the entire group of fat-soluble vitamins, quercetin, polyunsaturated fatty acids, some amino acids, and flavono-signals are found - about 200 biochemical components in total due to the multilateral use of milk thistle. Milk thistle is a plant for the treatment of, above all, the liver, stomach, intestines, although it has proven itself in diseases of the skin, cardiovascular disease, ear, throat, nose. With the help of milk thistle, a number of gynecological problems can be solved. It is also used in the treatment of hemorrhoids. The active ingredient of milk thistle is silymarin (a complex of flavonolignans - silibinin, etc.), which has a pronounced protective (protective) and restoring liver cells effect, has an antioxidant, detoxifying (neutralizing toxins and poisons) effect. Silymarin prevents the penetration of toxic substances into the cells of the liver and decomposes other poisons before they begin to exert their detrimental effect. This is the only currently known natural compound that protects liver cells and restores its function. Milk thistle extract is recommended by the RAMP Nutrition Institute as a prophylactic for ecologically disturbed regions. Milk thistle enhances the formation and secretion of bile, secretory and motor functions of the gastrointestinal tract, increases the protective properties of the liver in case of infections and various types of poisoning (including bleach, alcohol, radiation, medications, pale grebe and fly agaric), milk thistle is also highly effective in case of fatty degeneration of the liver.

A known method for the production of homogenized brown algae by preliminary destruction of the brown algae kelp with its subsequent extraction and the addition of components provided for by the recipe. (one)

The disadvantages of this method is the loss of most oligosaccharides and monosaccharides contained in the raw materials, the instability of the resulting drug, poor biological activity, the presence of important contraindications and low attractiveness for organoleptic indicators.

A known method for the production of homogenized brown algae, in which the starting components: kelp or its concentrate, garlic, sodium alginate and beckon, are thoroughly mixed in the claimed proportions and subjected to granulation by the generally accepted method, garnut is dried at t not higher than 100 ° C, the target product is obtained in the form granules with a size of 2 mm, granules are packaged in capsules of 0.3 g. (2)

The disadvantages of this method are the loss of most polysaccharides, oligosaccharides and monosaccharides contained in the raw materials, poor storage stability of the resulting preparation, i.e. short (no more than 30 days) shelf life of the product, weak biological activity and the narrow range of functional therapeutic and preventive possibilities, due to the non-optimal technological process of preparation, in which a sufficient number of organ iodine complexes and calcium alginates are not formed, the composition and ratio of ingredients, short duration of action, low attractiveness by organoleptic indicators (unpleasant aftertaste). An important disadvantage of this drug is the low digestibility of its iodine. For example, in the Far Eastern regions of Russia, the population who consume seafood in abundance, including seaweed (kelp), is susceptible to diseases characteristic of iodine deficiency almost to the same extent as in the European part. This is due to the fact that the intake of products containing iodine in its natural form is not sufficiently effective. Such a drug, which contains mainly iodine in its natural form, is contraindicated in nephritis, hemorrhagic diathesis, and other conditions in which iodine preparations are undesirable. At the same time, the most easily digestible and valuable part (polysaccharides) not only is lost and reduces the value of the biogel, but also pollutes the technological effluents, increasing the load on the treatment plant.

A known method for the production of biologically active products from brown algae, involving the cutting of raw materials, in which thalli are taken from fresh brown algae, cut rhizoids, washed with sea water from salt and solids and dried, and then washed in fresh water at a temperature of 25-35 ° C for 20-50 minutes with periodic stirring for uniform swelling by at least 30%, then algae are demineralized, for which they are poured with a 0.1-2% solution of at least one food acid from the group: limo natural, oxalic, phosphoric, acetic, dairy, succinic, hydrochloric or sulfuric, having an acidity of pH 2.0, with a mass ratio of algae and solution equal to 1: 1.5 and incubated for 0.5-10 hours at a temperature of 10- 50 ° C, after which the food-grade acid solution in which demineralization was performed is filtered, desalted and separated, and the algae are washed from the acid in fresh water for 10-12 minutes and soaked in drinking water at a temperature of 20-35 ° C for 30 -50 min, allow water to drain for 10-20 min, washed algae is crushed and placed in varo hydrochloric container with a stirrer and a homogenizer, added with at least a portion of said filtered and desalted solution of an edible acid, wherein demineralization is performed, drinking water with a temperature of 75-85 ° C and at least one edible alkali of the group. calcium carbonate, sodium bicarbonate, caustic soda, potassium hydroxide, magnesium carbonate, magnesium hydroxide to achieve a pH value of 7 to 12, which is controlled during cooking, carried out for 0.5-18 hours at a temperature of 40-100 ° C continuous stirring with a stirrer with a rotation speed of 15-25 rpm, after which it is cooled to 35-45 ° C and homogenized for 30 minutes, then neutralized, for which 0.1% citric acid solutions and edible calcium salt are introduced into the amount of 0.2% by weight of algae, mix thoroughly for of 10 min, heated to 75 ° C and converted into a form suitable for storage, containing 50-99% of water and at least 30% polysaccharides, based on dry substance. This method is used to minimize the loss of biologically active substances and to obtain the drug in the form of a stable complex with a high content of biologically active easily digestible substances (3).

The disadvantages of this method are the narrow spectrum of action (functionality) of the obtained product, low antioxidant properties, poor organoleptic characteristics (ridiculous combination of sweetness and minerality, aftertaste: weak unpleasant bitterness), narrowing the volume of consumption and commercial attractiveness of the product, the need for long-term use for a significant restoration of performance with mental and physical overwork, insufficiently high inhibitory activity in relation to pathogenic microflora, relatively low efficiency in the case of exogenous intoxication with various poisons and significant violations of microbiocenosis, i.e. with a number of poisonings and diseases of the gastrointestinal tract, accompanied by impaired normal liver function, the possibility of a negative effect of iodine on kidney function, due to which the product, although to a lesser extent than the prototype, is also contraindicated in nephritis, hemorrhagic diathesis and other conditions, which iodine preparations are undesirable.

An object of the invention is the creation of an effective and inexpensive method for the production of homogenized brown algae with the addition of fruits in the form of a biologically active drug with a wide spectrum of action, which ensures the full recovery of the body through the rapid removal of toxins from all sections of the gastrointestinal tract (GIT), as well as expanding the arsenal of methods production of homogenized brown algae in the form of biologically active preparations

The technical result that provides a solution to the problem lies in expanding the spectrum of action (functionality) of the obtained product in terms of increasing the inhibitory activity against pathogenic microflora, enhancing the secretion and motor function of the gastrointestinal tract, increase the protective properties of the liver in relation to infections and various poisonings, prevention of cancer and heart and blood vessel diseases, treatment of urinary tract infections, counteraction to the development of atherosclerosis, p prevention and prevention of cellulite, combating intoxication in general and food intoxication, in particular, the formation of antioxidant properties, improving organoleptic characteristics due to the presence of sugars and food acids that are most biocompatible with the body, reducing the duration of intake for a significant restoration of working capacity during mental and physical overfatigue, and reducing negative effects on kidney function. The product obtained according to the present invention has additional unique properties that allow it to enhance the known effects of the ingredients included in the composition to normalize the microflora of the gastrointestinal tract and increase immunity, significantly increase the body's resistance to bacterial, viral, fungal (including yeast forms) infections, and also reduce the risk of cancer and degeneration processes in the mucous membrane of the gastrointestinal tract.

The essence of the invention lies in the fact that the method of producing biologically active products from brown algae involves the cutting of raw materials, in which thalli are taken from fresh brown algae, cut off rhizoids, washed with sea water from salt and solids and dried, and then washed in fresh water at a temperature 25-35 ° C for 20-50 minutes with periodic stirring for uniform swelling, not less than 30%, then algae are demineralized by pouring 0.1-2 %% solution of at least one food acid from the group: citric, oxalic, phosphoric, acetic, lactic, succinic, hydrochloric or sulfuric, having an acidity of pH = 2.0, with a mass ratio of algae and solution equal to 1: 1.5 and incubated for 0.5-10 hours at a temperature of 10-50 ° C, after which the food acid solution in which demineralization was carried out is filtered, desalted and separated, and the algae are washed from the acid in fresh water for 10-12 minutes and soaked in drinking water at a temperature of 20-35 ° C for 30-50 minutes, allow water to drain for 10-20 minutes, washed in sprouts are chopped and rubbed fruits from the group are added: cranberries, prunes, dried apricots, lingonberries, sea buckthorn, rosehip, milk thistle, in an amount up to 20% of the mass of algae, and placed in a cooking container with a mixing device and homogenizer, at least a part is added of the mentioned filtered and desalted food acid solution in which demineralization was performed, drinking water with a temperature of 75-85 ° C and at least one food alkali from the group: calcium carbonate, sodium bicarbonate, sodium hydroxide, potassium hydroxide, carbonate t of magnesium, magnesium hydroxide to achieve a pH value of 7 to 12, which is controlled during cooking, carried out for 0.5-18 hours at a temperature of 40-100 ° C with continuous stirring with a stirrer with a rotation speed of 15-25 rpm and then cooled to 35-45 ° C and homogenized by the action of centrifugal forces of the rotor with the blades together with hydroacoustic and hydrodynamic vibrations for 30 minutes, then they are neutralized, for which solutions of citric acid in the amount of 0.1% and edible calcium salt are introduced in the amount of 0 , 2% by weight of algae, mix thoroughly for 10 minutes, heat to 75 ° C and convert to a form suitable for storage with a content of 50-99% water and at least 30% polysaccharides in terms of dry matter.

Preferably, the edible acid solution that was demineralized is filtered and desalted by dialysis or on semi-permeable or diffusion or osmotic membranes with a selectivity of 50-1500 Da, the filtered and desalted edible acid solution in which the demineralization is carried out is separated for use as a biologically active product or component of a biologically active product, a solution of food acid, which was used to demineralize algae, is reused for demineral algae up to 15 times, as algae take algae from the group: kelp and fucus from the basin of the Okhotsk, Bering or Sea of Japan.

In this case, the rhizoids are cut manually or on circular knives, and the thalli are washed with sea water in a cascade or vortex washing or by showering; if spirorbis is present on the surface of the thalli, they are brushed, dry algae are washed in fresh water by showering in the bath, washing from sand and salts, for washing use natural drinking water with a hardness of up to 1 mol / m ³ or softened (corrected) drinking water with a pH of not more than 7.8 and hardness of up to 0.2 mol / m ³ , washed algae are ground in a meat grinder or washing machine, for cooking washed algae is crushed and filled with hot water in a cooking vessel with a ratio of input heating power and usable volume equal to 0.20-0.25 kW / l, for example, in a reactor with a thermostatically controlled jacket and stirrer having a cutting edge for heating dry steam, or in an electric boiler with a stirrer, and homogenized in the built-in or remote homogenizer for 30 minutes, when neutralized, flavor additives are added to the mass, and immediately before cooking, add steviazide in an amount of up to 1 mg per 1% of the mass of algae, before converting into a form suitable for storage, the gel is enriched with polyvalent metal salts of the group: magnesium chloride, magnesium sulfate, calcium chloride, calcium citrate, calcium lactate in the calculation of a final concentration of 0.001- 1% in the gel, which is controlled for the iodine content, which should be at least 15 mg / kg, of carbohydrate fractions, which should be: mannitol - 245.4 ± 12.1 mg / g, alginic acid - 186.1 ± 10, 7 mg / g, check the hygienic characteristics in terms of toxic elemental content ents: lead - not more than 0.5 mg / kg, arsenic - not more than 0.5 mg / kg, cadmium - not more than 1.0 mg / kg, mercury - not more than 0.1 mg / kg, DDT - not more than 2.0 mg / kg, radionuclides - not more than 6 kg / kg, for transformation into a form suitable for storage, the gel is Packed in hot form or pre-drying, and drying is carried out using a freeze dryer or vacuum drying unit or installation spray drying or convection drying installation, while the prepared pasteurized mass is poured on a stretch or in cuvettes with a layer not Lee 10 mm which are placed on shelves in a drying apparatus, wherein the mass is dried to the desired final moisture content.

The method is implemented as follows.

When implementing the method, the following ingredients are used:

- kelp according to GOST 20438 (or sea kale - raw according to TU 15-01-360),

- food fucuses according to OST 64-026-87;

- fruits from the group: cranberries, prunes, dried apricots, lingonberries, sea buckthorn, dogrose, milk thistle, according to the current regulatory and technical documentation;

- acetic acid according to GOST 61-75;

- food citric acid according to GOST 908-2004;

- hydrochloric acid according to GOST 3118 or according to GOST 3857;

- oxalic acid according to TU 2431-001-55980238-02;

- phosphoric acid according to GOST 6552-80;

- lactic, succinic, sulfuric acid according to current regulatory and technical documentation or obtained by import;

- calcium carbonate, sodium bicarbonate, sodium hydroxide, potassium hydroxide, magnesium carbonate, magnesium hydroxide according to TU 113-08-667-98, GOST 4201, TU 6-09-01-263-85 and TU 6-09-3839;

- steviazide according to current regulatory and technical documentation or obtained by import;

- drinking water according to GOST R 51232 with hardness up to 1 mol / m or softened (corrected) drinking water with a pH of not more than 7.8 and hardness up to 0.2 mol / m ³ .

Calcium carbonate is a means of improving brain activity, and lowering blood sugar, lowering the acidity of gastric contents, and eliminating heartburn.

Sodium bicarbonate (sodium bicarbonate) is an antacid. When taken orally, it interacts with HCl of gastric juice, neutralizing it. Increases excretion of Na + and Cl-, osmotic diuresis; shifting the reaction of bronchial mucus to the alkaline side, increases bronchial secretion, reduces the viscosity of sputum and improves its expectoration. Eliminates pain in peptic ulcer and 12 duodenal ulcer. Alkalization of the urine prevents the precipitation of uric acid in the urinary tract. In its pure form, as a result of the neutralization reaction, CO ₂ can cause discomfort in the stomach and burping.

Calcium citrate (calcium citrate) (E331), chemical formula [ОСС (ОН) (СН2СОО) 2] 2Са3 * 4H2O - four-water calcium citrate - calcium preparation, approved and recommended for use in the food industry. The products are accompanied by the Sanitary and Epidemiological Report of the Ministry of Health of the Russian Federation.

It has been established that calcium non participate in the excitation and contraction of muscle cells, ensuring cell permeability, intercellular interactions, blood coagulation, secretion of hormones, mediators, and enzymes. Calcium ions act as a transformer of information that enters the cell, regulate intracellular metabolism, including energy. From the extracellular space they penetrate into the protoplasm through special calcium channels, affecting various physiological processes, the functions of the cells of all organs, vascular tone, systole intensity, diastole. Calcium ions play an important role in the formation and preservation of bone tissue. Calcium citrate replenishes the lack of calcium ions in the body with functional (physiological increased need as a result of growth, normal blood loss in women, leaching of bone calcium in elderly people, etc.) and pathological (trauma, bleeding, malabsorption in the gastrointestinal tract , inability to reabsorb calcium in the kidneys, etc.) insufficiency of replenishment of the body with calcium ions. When using the drug in its pure form, loss of appetite and abdominal pain may occur.

Caustic soda, caustic potassium are widespread preparations of the food industry.

Magnesium carbonate, magnesium hydroxide - antacid drugs, acting mainly in the field of sensitive (afferent) nerve endings. Form colloidal solutions with water and neutralize hydrochloric acid of gastric juice and other irritating substances. Magnesium hydroxide interacts with hydrochloric acid of gastric juice with the formation of magnesium chloride, the latter acts in the intestines as a salt laxative.

Calcium lactate is a calcium preparation approved and recommended for use in the food industry. The products are accompanied by the Sanitary and Epidemiological Report of the Ministry of Health of the Russian Federation.

Calcium lactate regulates calcium-phosphorus metabolism, has anti-inflammatory, anti-rheumatic, vitamin and hemostatic effects. It accelerates all phases of blood coagulation, increases platelet adhesion, and promotes the rapid entry of tissue thromboplastin into the blood. When using the drug, heartburn can occur.

Stevioside - a natural sweetener - Stevia honey herb extract (Paraguay). Stevioside - approximately 200-300 times sweeter than sugar, calorie-free, does not cause caries. Stevioside improves the functioning of the cardiovascular, immune and other systems; thyroid gland, liver, kidneys, spleen. It normalizes blood pressure, has anti-oxidant, adaptogenic, anti-inflammatory, anti-allergenic and moderate choleretic effect. It inhibits the development of many microorganisms and is therefore recommended for diseases of the oral cavity: it protects teeth from caries, and gums from periodontosis, which is a common cause of tooth loss, including diabetes mellitus, helps to prevent bad breath.

Cranberries, frozen, IQF, peeled, according to GOST 19215-73

Prunes according to GOST 28501-90 or according to TU 9164-005-57889401-2004

Dried apricots according to TU 9164-002-47944079-98

Fresh lingonberry according to GOST 20450-75

Sea buckthorn mature Altai Territory with a certificate of conformity

Rose hips according to GOST 1994-36

Ripe sunflower seeds of milk thistle. Kirov region with certificate of conformity

In the manufacturing process, the following processing steps are performed.

1. The preparation of algae.

As brown algae, algae are taken from the group: kelp and fucus from the basin of the Sea of Okhotsk, Japan or the Bering Sea, outside the zones of industrial and household waste dumping. The production of algae is carried out in accordance with Instruction No. 6, approved by order of "DALRYBA" No. 466 of July 10, 1985.

Use algae - raw or dried, approved for use in the food industry GSEN RF.

2. Cutting.

Fresh brown algae take thalli (sea stalk), cut rhizoids (growths) manually or on circular knives. Produce unfit plants.

3. Washing.

Thalli are washed with sea water in a cascade or vortex washing or by showering. If there are sprorbis thalli (shells) on the surface, they are cleaned manually with brushes, followed by washing.

After washing the thallus, a paired tray is placed to swell the water and dry it. Dry algae are washed in fresh water, in the bath by washing by washing from sand and salt. After that, at a temperature of 25-35 ° C give exposure for 20-50 minutes with periodic stirring for uniform swelling, at least 30%.

4. Demineralization.

Algae is poured into a 0.1-2% solution of at least one food acid from the group: citric, oxalic, phosphoric, acetic, lactic, succinic, hydrochloric or sulfuric, having an acidity of pH = 2.0, with a mass ratio of algae to solution equal to 1: 1.5 and incubated for 0.5-10 hours at a temperature of 10-50 ° C. A solution of food acid, which was used to demineralize algae, is usually reused for demineralization of algae from one to 15 times. During demineralization, part of the biologically active substances inevitably passes into the solution: polysaccharides, mainly mannitol, alginic acid, which is an intercellular substance and one of the components of the cell walls of algae

After completion of the use of this solution for demineralization, it is filtered and desalted by dialysis on either semipermeable or diffusion or osmotic membranes with a selectivity of 50-1500 Da. The filtered and desalted food acid solution in which demiperalization was performed, i.e. acid extract, contains polysaccharides extracted from demineralization from algae, mainly mannitol, alginic acid. This solution is separated (collected) in special containers as an independent biologically active product.

In contrast to conventional practice, the food acid solution that demineralized algae does not merge. As a result, the biologically active substances present in it are not lost and the load on the sewage treatment systems is reduced.

The filtered and desalted food acid solution in which demineralization was carried out can be fully or partially used independently as a biologically active product, as well as as a component for enriching a variety of biologically active products.

As a component for enrichment of a biologically active product, such a filtered and desalted solution of food acid is necessarily used in the present inventive method.

5. Flushing.

After the specified time, algae are washed from acid in fresh water by strangling for 10 minutes and soaked in a hopper in drinking water at a temperature of 20-35 ° C for 30-50 minutes with 1-2-fold change of water during the soaking process.

After the last washing, the container with algae is lifted from the hopper and allowed to drain water for 10-20 minutes.

6. Shredding.

The washed algae is crushed in a meat grinder M-250, MIM-300, MIM-600, TJI2H (made in China) or MPR-350.01 rubbing machine. Rubbed fruits from the group are added: cranberries, prunes, dried apricots, lingonberries, sea buckthorn, dogrose, milk thistle, in an amount up to 20% of the fruit by weight of the algae, and loaded into a cooking container.

7. Cooking.

The crushed algae and fruits are placed in a cooking vessel with a ratio of supplied heating power and usable volume equal to 0.040-0.045 kW / l, for example, a 1050 l reactor having electric heaters with a capacity of 45 kW and made with a cutting room for heating with dry steam, or in an electric boiler with a stirrer (with a similar ratio of power of electric heaters and volume) and with a thermostatically controlled jacket At least a portion of said filtered and desalted food acid solution is poured into the reactor in otor was demineralized (acidic extract), and hot drinking water at a temperature of 75-85 ° C in relation to the algae mass 1: 1, at least one food alkali from the group was added, calcium carbonate, sodium bicarbonate, sodium hydroxide, potassium hydroxide, magnesium carbonate, magnesium hydroxide to achieve a pH value of 7-12, which is controlled during the cooking process, and stevioside up to 1 mg for each percent of the mass. Cooking is carried out for 0.5-18 hours at a temperature of 40-100 ° C with continuous stirring with a stirrer with a rotation speed of 15-25 rpm.

8. Homogenization.

от подведенной мощности N' затрачивается на создание полезного напора Н₂ с полезной подачей Q (объемным расходом) продукта через нее и, следовательно, части N₂~QH₂ полезно используемой, передаваемой продукту секундной механической энергии. Здесь и далее символ ~ означает пропорциональность. Напор Н₂ пропорционален разности параметра циркуляции (средних моментов абсолютной скорости) Г_k2 перед и Г_k1 на выходе лопаток ротора. Таким образом, уравнение баланса подведенной мощности и полезной секундной механической энергии передаваемой продукту имеет следующий общий вид:After cooking, the resulting mass is cooled to 35-45 ° C and homogenized for 30 minutes, for example, with an RPL brand homogenizer having a rotor with blades. Rotary pulsation apparatuses (RPA) combine the principles of operation of a hydrodynamic oscillation generator and a centrifugal pump. The supplied power N '= 30 kW from the rotor shaft, driven into rotation by an electric motor with a torque M' and angular velocity ω, is transmitted to the rotor blades. The blades with their inter-blade channels form together with the stator channels the flow part of the pump of the apparatus. Due to the hydrodynamic interaction of the blades with the processed product (working fluid) part $$N_2^{\text{'}\text{'}}$$

of the supplied power N 'is spent on creating a useful pressure Н ₂ with a useful supply Q (volumetric flow rate) of the product through it and, therefore, part N ₂ ~ QH _{2 of the} useful mechanical energy transferred to the product. Hereinafter, the symbol ~ means proportionality. The pressure Н _{2 is} proportional to the difference in the circulation parameter (average moments of the absolute speed) Г _k2 before and Г _k1 at the exit of the rotor blades. Thus, the equation of balance of supplied power and useful second mechanical energy transmitted to the product has the following general form:

$$N\text{'}\text{'}={\displaystyle \sum _{k=\mathrm{one}}^n{N}_k^{\text{'}\text{'}},n=2;\left(\mathrm{one}\right)}$$

$$N\text{'}\text{'}=M\text{'}\text{'}\omega ,N_k^{\text{'}\text{'}}=m_k\frac{\rho gQ}{{\eta }_m{\eta }_{ok}^{*}{\eta }_{gk}^{*}}\frac{H_k}{{\eta }_{ok}{\eta }_{gk}},m_k=\mathrm{one,}$$

Where:

$${\eta }_{ok}^{*}=\frac{Q}{Q+\Delta Q_k^{*}},\left(2\right)$$

$$H_k=\frac{\omega }{2\pi g}\left(G_{k2}-G_{k\mathrm{one}}\right).\left(3\right)$$

Equation (3) is equivalent to the expression

$$H_k=\frac{p_{k3}-p_{k\mathrm{one}}}{\rho g}+\frac{{\upsilon }_{u3}^2-u_{u\mathrm{one}}^2}{2g},\left(\mathrm{four}\right)$$

or using the Bernoulli equation for the relative motion of the product flow at the location of the rotor blades and the equation of absolute motion, as well as taking into account the relatively small differences in the potential of external mass forces (gravity) in these areas

$$H_k=\frac{{\upsilon }_{k2}^2+u_{k2}^2-w_{k2}^2}{2g}-\frac{{\upsilon }_{k\mathrm{one}}^2+u_{k\mathrm{one}}^2-w_{k\mathrm{one}}^2}{2g}-{\xi }_{k\mathrm{,one}-3},\left(5\right)$$

$${\xi }_{k\mathrm{,one}-3}=\frac{H_k}{{\eta }_{gk}}\left(\mathrm{one}-{\eta }_{gk}\right).$$

Reactive moments are determined from the equation

$$M_{pk}=\frac{\rho Q}{2\pi {\eta }_0}\left(G_{k3}-G_{k2}\right).\left(6\right)$$

In equations (3), (6), the circulations Г _{ki are} determined through the moments of absolute velocity by the relation

$$G_{ki}=2\pi {\left(r{\upsilon }_u\right)}_{ki},i=\mathrm{1,2,3.}\left(7\right)$$

- гидравлический коэффициент полезного использования участков проточных частей корпуса 1 вне рядов ступеней лопаток, η_м - механический кпд аппарата, η_0k, η_rk - объемный и гидравлический кпд насоса, а $$\Delta Q_k^{*}$$

- рзсход перетечек вокруг лопаток ротора, $$\Delta Q_k^{*}=\Delta Q$$

, где ΔQ - расход перетечек вокруг поверхностей статоров 13, 14; (rυ_u)_ki, p_ki, υ_ki, u_ki, w_ki, - осредненные по площадям проточной части корпуса 1, соответственно, момент скорости, давление, модули векторов абсолютной $$\overrightarrow{\upsilon }$$

, переносной $$\overrightarrow{u}$$

(u=ωr, r - радиус точки), относительной $$\overrightarrow{w}$$

скоростей перед (индекс i=1) и за лопатками ротора, т.е. перед лопатками статора (i=2), а также за лопатками статора (i=3), υ_u - окружная компонента абсолютной скорости $$\overrightarrow{\upsilon }=\overrightarrow{u}+\overrightarrow{w}$$

; ξ_k,1-3 - гидравлические потери.In the expressions (1) - (7), the following notation is used: n - number of blades 29; m _k is the number of steps; k is the index of the number of steps; ρ is the density of the working fluid, g is the acceleration of gravity, $${\eta }_{gk}^{*}$$

- hydraulic efficiency of sections of flowing parts of the housing 1 outside the rows of stages of the blades, η _m - mechanical efficiency of the apparatus, η _0k , η _rk - volumetric and hydraulic efficiency of the pump, and $$\Delta Q_k^{*}$$

- rzshod flow over the blades of the rotor, $$\Delta Q_k^{*}=\Delta Q$$

where ΔQ is the flow rate of the overflows around the surfaces of the stators 13, 14; (rυ _u ) _ki , p _ki , υ _ki , u _ki , w _ki , are the moment of speed, pressure, absolute vectors absolute $$\overrightarrow{\upsilon }$$

portable $$\overrightarrow{u}$$

(u = ωr, r is the radius of the point), relative $$\overrightarrow{w}$$

speeds in front (index i = 1) and behind the rotor blades, i.e. in front of the stator blades (i = 2), as well as behind the stator blades (i = 3), υ _u is the peripheral component of the absolute speed $$\overrightarrow{\upsilon }=\overrightarrow{u}+\overrightarrow{w}$$

; ξ _{k, 1-3} - hydraulic losses.

The diverted product has second mechanical energy acquired as a result of the implementation of the stated working process

$$N=\rho gQ{\displaystyle \sum _{k=\mathrm{one}}^n{m}_k{H}_k,n=2m_k=\mathrm{one.}\left(8\right)}$$

This energy, due to hydrodynamic effects and hydroacoustic effects associated with the phenomena of water hammer and cavitation, accompanying the rotation of the rotor 11, is completely converted into deformation of the product and its heating,

By means of pulsating, shock and hydrodynamic effects, by the action of centrifugal forces, together with the hydroacoustic and hydrodynamic vibrations occurring in RPA, the physicomechanical properties of the product are changed, which is converted into the most effective and convenient form for the body to absorb. In the process of homogenization, the particles are crushed to about one micron, uniformly distributed in the mass of the product. Under the influence of pulsating, shock and hydrodynamic effects, together with hydroacoustic and hydrodynamic vibrations, processes of dispersion, emulsification, dissolution, extraction, oxidation, polymerization are carried out. At the same time, intense acoustic exposure leads to the destruction of bacteria and microorganisms at this stage. Released substances of peptide nature are able to regulate the various stages of proliferation and differentiation of T-lymphocytes and lymphocytes, that is, act on the links of the immune response. The homogenized product does not exfoliate during prolonged storage, suitable for transportation, while maintaining its physiological qualities.

9. Neutralization

To neutralize, 0.1% citric acid solutions and 0.2% edible calcium salt are added to the homogenized mass, mixed thoroughly for 10 minutes, and citric acid solutions are added in an amount of 0 to 75 ° C; the homogenized mass is added. , 1% and edible calcium salt in an amount of 0.2% by weight of algae. As a solution of edible calcium salt, a solution of calcium lactate or calcium citrate is introduced. At this stage, flavoring additives can be introduced (according to a specific formulation, e.g. lemon or cumin liss in the amount of 0.05% by weight of algae). The mass is thoroughly mixed for 10 minutes. During processing, polysaccharides, mainly mannitol, alginic acid, which is an intercellular substance and one of the components of the cell walls of algae, are released. The introduced substances form complex compounds with algae homogenization products, mainly mannitol and sodium-calcium algae. Most of the iodine is in the form of organic iodine compounds.

After that, the mass is heated to 75 ° C (pasteurized) and converted into a form suitable for storage with a content of 50-99% water and at least 30% polysaccharides in terms of dry matter, and then, for example, packaged in hot form or served on drying.

10. Drying.

For drying, use the freeze-drying unit P1-ISS-101, a unit of type TG-15 or TG-50 (Germany) or vacuum drying SPT-200, spray drying "Niro atomizer" or convection drying IP-120 (Hungary).

The prepared pasteurized mass is poured on a protevia or in cuvettes with a layer of not more than 10 mm, which are placed on shelves in a drying unit, where the mass is dried. The final moisture content of the product should not exceed 10%.

11. Packing.

11.1. Packing in a dry form.

The finished powder of the drug is packaged in heat-sealed film bags according to TU 15-1106-90 with a maximum weight of 100 g or in other containers made of materials allowed for contact with foodstuffs of the RF State Sanitary and Epidemiological Supervision and ensuring the safety of products.

11.2. Packing hot.

The mass is Packed in hot form with a dispenser UD-2 or UFM in sealed containers and cooled.

As a result of processing by the claimed method, the product is a combination of large formations - supramolecules, consisting of a large, but certainly finite number of molecular oligomers. At the same time, supramolecular ensembles that belong to clathrates are polymolecular systems that arise as a result of spontaneous association of components and have a certain spatial organization, which is associated with unique physico-chemical properties The formation of supramolecules is based on complement ntarnosti (complementarity applied algae and fruits by treatment) Upon binding its constituent molecules in clathrates there are certain interactions that mediate the molecular ordering in the space blocks and formed supramolecular "architecture". In supramolecules, the retention of individual fragments occurs due to non-valent intermolecular interactions, which include hydrogen bonds, electrostatic forces, lyophilic and lyophobic interactions.

Due to this, the obtained gel retains practically all biologically active substances of the raw material in a bioavailable form and contains 92-94% of water, 6-8% of dry substances, which include fructose, glucose, sucrose, pectin, tannins and nitrogenous substances, minerals - a large amount of potassium, sodium, calcium, magnesium phosphorus, organic acids from the group: malic, citric, oxalic, salicylic, benzoic, ursolic and also alginic acid 5-6% in the form of sodium-calcium alganate, fiber - 1-1.5 %, protein - 1%, mineral micro- and macronutrients: molybdenum (12 × 10 - 3%), manganese (4.4 × 10 - 3%), iron (13 × 10 - 3%), vitamins A, C, B1, B2, P, a complex of flavonoids and flavonoligians, fucoidan, stevioside and others. Nitrogenous substances are represented by 17 amino acids, among which 7 are essential. The resulting product has a complex effect that exceeds the total effect of the separately taken algae and fruits, which is due to the interaction of the substances of the fruits with iodine and other components of the algae activating each other. The resulting product is a flexible sliding texture, the taste is full and voluminous, with tones of fruits (fruits), good balance acid, tonic aroma with the nuances of contained fruits (fruits). Aftertaste: pleasant, fruity, moderately fresh. Preservation (stability) of properties was controlled during 12 months of storage at a temperature of 8-12 ° C in appearance - preservation of taste, color, texture and smell, the absence of delamination, swelling, gas formation. No changes in these indicators during storage were found.

The following are examples of the practical use of the gel obtained by the claimed method.

Example 1

The study of the effects of the gel obtained by the claimed method in diseases of the gastrointestinal tract was carried out on the following groups of patients: patients of the experimental group - 36 people (18 men, 18 women) with an average age of 52 ± 10 years. The control group was 25 people aged 54 ± 7 years.

Patients complained about; a feeling of heaviness in the right hypochondrium, nausea, belching, heartburn, epigastric pain, indigestion in the form of diarrhea or constipation. In the study of laboratory parameters in a number of patients, an increase in the level of ALT, LDH, ACT, and bilirubin was detected due to the indirect fraction. The coprogram has an increased amount of fatty acids.

The indicators of the coprogram before and after the use of the gel prepared according to the present invention (in% of the total number of cases)

No. Indicators no does not mean. moderately much before after before after before after before after one. Muscle fibers 0 0 57 55 37 40 7 four 2. Neutral fat 66 74 24 17 9 8 0 0 3. Fatty acid 22 eleven 40 39 22 21 16 25 four. Soaps fifty 52 28 22 19 twenty 3 0 5. Indigestible fiber 31 9 36 34 24 51 10 7 6. Digestible fiber 44 32 31 43 fifteen 10 9 12 7. Starch 17 24 52 51 21 eleven 12 fifteen

The experimental group within 21 days, along with drugs generally accepted in similar cases, received 50 ± 5 g of gel produced by the claimed method in 30 minutes. before meals in the morning and evening. Of these, 5 people received a product for the preparation of which kelp, cranberries, magnesium chloride and calcium lactate were used, the other 5 people received a product for the preparation of which kelp and fucus were used in a mass ratio of 1: 1, lingonberry, and as edible calcium salt - calcium citrate, in this batch, stevioside was introduced into the composition of the product. Another 4 people received a product for the preparation of which kelp, dried apricots, magnesium chloride and calcium lactate were used, the other 4 people received a product for the preparation of which kelp and fucus were used in a mass ratio of 1: 1, dried apricots, and citrate as a dietary salt calcium, in this batch, stevioside was introduced into the product. In addition, 5 people received a product for the preparation of which kelp, prunes, magnesium chloride and calcium lactate were used, the other 4 people received a product for the preparation of which kelp and fucus were used in a weight ratio of 1: 1, sea buckthorn, and calcium as a salt - calcium citrate, in this batch, stevioside was introduced into the composition of the product. The shelf life of these products was 6-8 months. The control group received only standard drug treatment and the well-known drug containing kelp, Lamisan-L.

Patients of the experimental group, starting from the 5-6th day of the course, noted a steady improvement in overall health: normalization of appetite, decreased heartburn, belching, nausea, epigastric pain and heaviness in the right hypochondrium, and normalization of stool. Subjective improvement was confirmed by the positive dynamics of objective indicators in the experimental group. In 85% of patients, palpation sharply decreased or completely no pain in the epigastrium and in the projection area of the duodenal bulb, as well as along the intestine and in the right hypochondrium in the zone of the liver and gall bladder. In the control group, positive dynamics were weakly expressed, patients' well-being improved, as a rule, in a longer time frame (later by 9-16 days). Such signs as a feeling of heaviness in the right hypochondrium, skin itching, leg pain, dyspeptic symptoms, in the experimental group were stopped in a shorter time period than in patients of the control group.

In the biochemical analysis of patients of the experimental group receiving all product variants, the total protein and bilirubin content had a clear tendency to normalize. In the control group, positive changes were less pronounced and occurred at a longer time. Girdle pains, dyspeptic symptoms in patients of the experimental group, were stopped earlier than in patients of the experimental group. None of the patients noted unpleasant sensations in the stomach and belching, unpleasant aftertaste or other unpleasant phenomena.

In patients of the experimental group who received any of the mentioned product options, by the 15-18 day of the course, an improvement in the ultrasound picture of the liver was noted. In the control group, the ultrasound data at this moment remained without significant changes. Over the next 6 months, most patients did not have complaints characteristic of the moment the course of taking the gel obtained by the claimed method began.

Example 2

The study of the antibacterial properties of the gel obtained by the claimed method was carried out on 56 patients who were divided into two main groups - 36 people and the control group - 20 people. The average age of patients (in both groups) was 52 ± 3 years. These groups were identical in age and disease standard in accordance with ICD-10.

At the beginning of the course, an endoscopic examination for chronic constipation revealed bacterial-induced structural changes in the colon mucosa, which were expressed in focal inflammatory (55%) changes, as well as erosion on the colon mucosa (24%). Morphological changes in the colon mucosa were found in all patients. There were violations of the ratio of opportunistic and saprophytic intestinal microflora, opportunistic microflora were sown in 81% of patients. In patients of the control and experimental groups in the analysis of feces for dysbiosis, the presence of Staphylococcus aureus and an increased titer of E. coli was revealed. Three from the experimental group suffered from nephritis, four from urolithiasis and had kidney stones confirmed by ultrasound.

The experimental group for 30 days, along with standard in similar cases, medicines received 70 ± 5 g of gel 30 minutes before meals in the morning and evening. The control group received only standard drug treatment. In the experimental group, 6 people received a product whose shelf life was 6 months, for the preparation of which kelp, cranberries, magnesium sulfate and calcium citrate were used, in this batch, stevioside was introduced into the composition of the product, 6 people received a product whose shelf life was 7 months and 15 days, for the preparation of which kelp and fucus were used in a mass ratio of 1: 2, prunes, and calcium lactate as calcium edible salt, 6 people received a product whose shelf life was 8 months to prepare Laminaria, dried apricots, magnesium sulfate and calcium citrate were used, in this batch, stevioside was introduced into the product, 6 people - a product whose shelf life was 7 months, for the preparation of which kelp and fucus were used in a mass ratio of 1: 2, lingonberry, and as a dietary salt of calcium - calcium lactate, 6 people received a product whose shelf life was 8 months, for the preparation of which kelp, sea buckthorn, magnesium sulfate and calcium citrate were used, in this batch the product was introduced I am stevioside, another 6 people - a product whose snoring period was 8 months, for the preparation of which kelp and fucus were used in a mass ratio of 1: 2, rosehip and milk thistle in a mass ratio of 1: 1, and calcium lactate as calcium salt.

Laboratory and instrumental methods of treatment and examination were carried out in accordance with the standards.

According to endoscopic and morphological studies during the course, the patients of the experimental group showed a positive dynamics of changes in the colon mucosa, which was expressed in a decrease in inflammatory (86%) and elimination of erosive changes in the colon mucosa (90%), reduction in cell infiltration (78% ) patients. Under the influence of a brown algae gel, chemical changes in feces normalized. PH normalized in 95% of patients, organic acids and ammonia normalized in 96%, mucin remained in feces only in 3% of patients, tissue protein was not noted. In all patients of the experimental group, a scarring of the ulcer defect occurred in a shorter period than in the control, and cicatricial deformity was less pronounced. The positive dynamics of the controlled parameters of patients suffering from nephritis and urolithic diathesis practically did not differ from the dynamics of other patients, no deterioration of other indicators was found. There was practically no protein content in the urine after 20 days of gel administration, by the end of the ultrasound course a sharp decrease in the number and size of stones to , allowing to count on their independent departure. Patients noted a pleasant taste and smell of the product, the absence of an unpleasant aftertaste. Over the next 12 months, most patients did not show signs of dysbiosis and exacerbation of urological and other diseases characteristic of the start of the course of taking the gel obtained by the claimed method.

Example 3

The effectiveness of treatment of iodine deficiency complicated by urolithiasis and gall bladder stones was evaluated in two groups of pregnant women. 1 group experienced of 48 people, 2 group control of 40 people.

The dynamics of the content of blood iodides (μmol / l) in pregnant women after taking the gel prepared according to the present invention

Examination Period (weeks) 8-12 (initial) 16-24 28-32 34-38 11.7 ± 3.6 18.2 ± 4.5 20.2 ± 3.0 22.5 ± 2.0 Experienced group Control group 11.8 ± 2.2 13.1 ± 3.5 12.4 ± 2.5 14.0 ± 2.5

Significant differences were found in the content of blood iodides before and after treatment of iodine deficiency in women. The experimental group received complex treatment corresponding to the form of the anemic condition, and an additional iodine-containing therapeutic and prophylactic product - a gel from brown seaweed, 50-1: 5 g of gel per 30 minutes. before meals in the morning and evening for 12 days before each control study. Of these, 16 women received a product with a shelf life of 4 months, for the preparation of which kelp, rosehip and milk thistle were used in a mass ratio of 1: 1, magnesium sulfate and calcium citrate; in this batch, stevioside was introduced into the composition of the product, and another 17 women received the product, term the storage of which was 6 months, for the preparation of which was used fucus, cranberries and dried apricots in a mass ratio of 1: 1, magnesium sulfate and calcium lactate, in this batch stevioside was introduced into the composition of the product, the remaining 15 women - a product whose shelf life was was 6 months, which were used for the preparation of Fucus and Laminaria in a weight ratio of 2: 1, lingonberry, seabuckthorn, and prunes in a weight ratio of 1: 1: 1, magnesium chloride and calcium citrate. Patients receiving the product noted a pleasant taste, lack of discomfort in the stomach, loss of appetite, and abdominal pain. The protein content in the urine normalized after 20 days of taking the gel (in accordance with the gestational age), by the end of the ultrasound course, a sharp decrease in the number and size of stones was recorded to a value that allows them to calculate their independent discharge. The improvement in the experimental group for all women who received any of the product options was stable and remained between the courses of taking the product.

The control group also received complex treatment depending on anemia and one of the preparations containing kelp was Laminarid or Lamisan-L (water kelp concentrate), which were practically equivalent to the prototype. The control was carried out by determining the activity of iodides in whole blood by direct potentiometry using ion-selective electrodes of the company "SguSh" (Czech Republic). Normally, these indicators are 20-50 μmol / L. Initially, a comprehensive clinical and laboratory study was carried out when registering in a antenatal clinic in the early stages. Women who had up to 12 weeks of gestation with a blood iodide content of about 12 μmol / L were selected in the experimental and control groups. During the course, comprehensive clinical and laboratory studies were carried out at 16-24, 28-32, 34-38 weeks of pregnancy. In the first group, upon receipt of the product - gel from brown seaweed, an increase in the content of blood iodides to values near the lower limit of the norm is noted. In women who received a well-known product, only a slight improvement was recorded, which at the end of the course remained significantly below normal, i.e. iodine deficiency was not compensated slightly, the phenomena of urolithiasis remained practically unchanged.

Example 4

Evaluation of the effectiveness of treatment in patients with cardiovascular diseases was carried out in two groups. 1 group of 56 people (men and women) - experimental, 2 group of 29 people (men and women) - control.

The first (control) group received complex treatment and a prophylactic product within 45 days — a gel prepared according to the present invention, 50 ± 5 g of gel 30 minutes before meals in the morning and evening. Of these, 19 people received a product whose shelf life was 7 months, for the preparation of which kelp, sea buckthorn, magnesium sulfate and calcium chloride were used, stevioside was introduced into the composition of the product, 20 people - a product whose storage period was 8 months, the preparation of which used kelp and fucus in a mass ratio of 2: 1, cranberries, magnesium sulfate and calcium lactate, 17 people - a product whose snoring period was 8 months, for the preparation of which kelp and fucus were used in a mass ratio of 2: 1, rosehip and milk thistle in a mass ratio of 1: 1, magnesium sulfate and calcium lactate.

The second (experimental) group received complex treatment and one of the well-known drugs containing brown algae, Lamisan-L or Laminarid.

As a result of the course administration of the gel prepared according to the present invention, against the background of a low-calorie diet (group 1), body weight decreased on average from 90.1 ± 0.4 to 80.3 ± 0.4 kg, p <0.05, whereas in the control group, weight loss was unreliable.

The increased fasting blood glucose level decreased from 6.5 ± 0.5 to 4.9 ± 0.1 mmol / L, p <0.05; in the control group, from 6.2 ± 0.15 to 5.9 ± 0.1 mmol / L, p> 0.1.

The prothrombin index in the 1st group decreased from 96.6 ± 2.4 to 85.21 ± 1.6%, p <0.01; in the control group, only from 102.9 ± 0.7 to 101.4 ± 0.5%.

An improvement in the blood lipid spectrum was noted, which was manifested in a decrease in total cholesterol from 6.25 ± 0.2 to 5.12 ± 0.31, p <0.05, in the control - from 7.14 ± 0.30 to 6.92 ± 0.3 mmol / l, p> 0.1; triglycerides - from 1.4 ± 0.22 to 1.2 ± 0.14, p <0.05, in the control - from 1.8 ± 0.45 to 1.59 ± 0.7 mmol / l, p> 0.1; / 3-lipoproteins - from 6.75 ± 0.2 to 5.9 ± 0.16, p <0.1, in the control - from 7.3 ± 1.45 to 7.18 ± 1.55 g / l p> 0.1; low density lipoproteins decreased in the 1st group from 3.46 ± 0.26 to 2.55 ± 0.1, p <0.02, in the control group from 5.4 ± 0.25 to 5.1 ± 0, 3 mmol / L, p> 0.1.

A single systolic pressure, measured in the morning, decreased from 155 ± 3 to 125 ± 2 mm Hg, p 0.01; in the control group, only from 155 ± 2 to 150 ± 2.0 mm Hg, p> 0.1; diastolic - from 92.0 ± 2, to 78 ± 2, p <0.0 1; in the control group from 97.0 ± 3 to 95.3 ± 2.0, p> 0.1.

The results above demonstrate a more effective normalizing effect of the drug obtained by the claimed method on the pathogenetic links of cardiovascular diseases that has occurred in patients receiving any of the product options. Control tests taken 3 and 6 months after the completion of the course showed that these indicators did not change compared to the moment the course was completed. Patients noted a pleasant taste and smell of the product, the absence of an unpleasant aftertaste or dyspeptic symptoms.

From the above examples and other studies, the therapeutic and prophylactic properties of various components of the gel prepared according to the present invention, as well as its potential in regenerative medicine, as a means of general healing of the body and maintaining a health reserve, as well as a product used in the complex treatment of diseases, are visible. The negative consequences of taking the drug have not been identified, i.e. side effects (which are the basis for establishing contraindications) were not found The result of taking the drug is stored for a period of at least four times the duration of the course of administration. Confirmed by tests at a temperature of 6-12 ° C, the shelf life of the properties is 8 months.

The action of the drug was tested by workers of 10 construction crews working at a construction site located in the area of coal-fired CHP emissions. For five weeks, employees of six experimental teams orally received the gel obtained according to the present invention, 50 ± 10 g of gel for 30-40 minutes. before meals in the morning and evening. In a team of 8 people received a product with a shelf life of 6 months, for the preparation of which kelp, cranberries, magnesium sulfate and calcium citrate were used, stevioside was introduced into the composition of the product, in a team of 7 people a product with a shelf life of 7 months, for the preparation of which kelp and fucus were used in a mass ratio of 1: 2, prunes, and calcium lactate as calcium salt, in a team of 7 people received a product whose shelf life was 8 months, for the preparation of which was used kelp, dried apricots, magnesium sulfate and calcium citrate, stevioside was introduced into the composition of the product, in a team of 6 people - a product whose shelf life was 7 months, for the preparation of which kelp and fucus were used in a mass ratio of 1: 2 lingonberry, and calcium lactate as a dietary salt of calcium, in a team of 6 people received a product whose shelf life was 8 months, for the preparation of which kelp, sea buckthorn, magnesium sulfate and calcium citrate were used, of this batch, stevioside was introduced into the product, in a team of 8 people - a product whose shelf life was 8 months, for the preparation of which kelp and fucus were used in a mass ratio of 1: 2, rosehip and milk thistle in a mass ratio of 1: 1, and as edible salt of calcium - calcium lactate.

Two control teams of 10 and 12 employees did not receive any therapy, another two control teams of 10 and 14 employees received the well-known drugs Laminarid and Lactofiltrum (dietary supplement tablets containing the composition of lactulose (110 mg) and hydrolysis lignin (350 mg) ) in the same dosage regimen as in the experimental teams 1-6.

In all teams, the incidence of gastrointestinal tract and acute respiratory infections before the start of taking the composition was 7.1-7.4%. After the indicated course of taking the composition in the experimental teams, the incidence of gastrointestinal tract and acute respiratory infections gradually decreased and by the end of the course amounted to 2.2-3.0% of the composition of the teams. Blood tests improved in 85% of cases. All patients noted a significantly improved taste and smell of the drug compared to any previously used drugs.

In the control teams that did not receive any therapy, the incidence of gastrointestinal tract and acute respiratory infections and blood tests remained at the same level as before the test, the control teams that did not receive gel, the incidence of gastrointestinal tract and acute respiratory infections remained at the initial level of 7.0-7.1% , blood tests improved in 10.5% of cases, which can be attributed to seasonal or other fluctuations.

The gel, prepared in accordance with the homogenization and composition of the present invention, is one of the most modern and high-quality biologically active products intended for independent, complex and auxiliary therapy, maintaining human health and the complex treatment of a wide range of diseases of the gastrointestinal tract, kidneys, liver, cardiovascular and endocrine systems, has enhanced antioxidant and immunomodulatory effects, as well as improved organoleptic characteristics mi and the minimum probability of side effects. A wide range of oligosaccharides, monosaccharides and ehnterosorbentov (plant fibers) that make up the seaweed used and fruits perform functions replacement, support, protective substances involved in immune reactions, provide adhesion of cells in the tissues of plants and animals. The therapeutic and prophylactic properties of oligosaccharides and, in particular, mannitol are determined by the fact that they are not digested in the upper parts of the gastrointestinal tract (they are not split by enzymes of the small intestine), but pass through into the large intestine, in which they are actively utilized by the normal intestinal microflora, in particular , lactobacilli and bifidobacteria. At the same time, enterosorbents and oligosaccharides absorb and neutralize toxic substances (lead, mercury, radionuclides, etc.) that enter the body from the external environment or are formed as a result of impaired metabolism, activate the process of cleansing the liver, blood vessels and heart walls, drain the intestines and restore its microflora.

In the large intestine, oligosaccharides are utilized under the influence of bacteria with the formation of low molecular weight organic compounds, mainly organic acids: lactic, acetic, butyric and propioic, which lead to a decrease in the pH of the intestinal contents. As a result of acidification of the intestinal contents, the osmotic pressure rises and the contents of the colon become thinner. . Organic acids produced by bifidobacteria inhibit the growth of proteolytic bacteria, respectively, reducing the quantitative indicators of toxic products of protein metabolism of scatol, p-cresol, indole and phenol, which improves the working conditions of the liver and heart. During the fermentation of polysaccharides, a more pronounced stimulating effect of the composition on the state of intestinal microbiocenosis is achieved. At the same time, acetic, formic, lactic and fatty acids are released in the large intestine to a large extent, which suppress the development of a wide range of conditionally pathogenic and pathogenic types of microorganisms (salmonella, shigella, fungi). These products, inhibiting the growth of proteolytic bacteria, dramatically reduce ammonia levels, reducing the load of toxic products on the liver and protecting the brain. Polysaccharide fermentation products additionally stimulate peristalsis, promote liquefaction of the chyme, eliminating constipation, and also reduce the burden on the kidneys, antioxidants and fruit vitamins stimulate immune reactions that destroy mutated cancer cells. In the presence of oligosaccharides, more intensive synthesis and assimilation of vitamins A and E by representatives of the normal intestinal microflora is observed.

The combination of a gel with the indicated content of prebiotic components (in particular, fructooligosaccharides or fructooligosaccharides together with lactulose) and the enterosorbent of the fruits, as substances with a predominant affinity for hydrophilic and amphiphilic toxic molecules and bacteria, gives additional dissolution and absorption properties of hydrophobic fatty acids in the entire digestive tract, starting with the oral cavity in an effective thin-emulsion form, as well as with hepatoprotective properties (strengthening the structure of the membranes of liver cells and a decrease in the content of endogenous metabolic toxins in the blood and cells, the property of inhibiting the growth and development of pathogenic microorganisms, which has the property of enhancing the body’s immunity and promoting the absorption of calcium in the intestine and reducing the phenomena of osteoporosis, made it possible to obtain a drug with fundamentally new properties that have the ability to bind throughout the digestive tract and remove pathogenic microorganisms, bacteria, yeast, fungi and viruses, and other harmful organisms without causing harm bacteria-friendly, help strengthen immunity, increase the effectiveness of the treatment of infectious intestinal diseases, reduce the risk of developing cardiovascular diseases (including by linking excess cholesterol) while improving the consumer’s characteristics of the drug, in form and composition suitable for use by children, starting from the age of 3-5 years.

Thus, an effective and inexpensive method for the production of homogenized brown algae in the form of a biologically active preparation of a wide spectrum of activity has been created, as well as an expanded arsenal of methods for the production of homogenized brown algae in the form of biologically active preparations.

The drug is intended for dietary, therapeutic and preventive nutrition, a source of polysaccharides and biogenic micronutrients. Unlike many known drugs, the proposed drug contains an increased amount of biologically active easily digestible substances with a molecular weight of 70 Da to 100 kDa: oligo - and monosaccharides (mainly mannitol) , amino acids and peptides, nucleotides and trace elements in the form of biogenic compounds, mainly with proteins and peptides, as it contains 50-99% water and at least 30% polysaccharide in on dry substance. Thus, the most easily digestible and valuable part is not lost, increases the value of the biogel, and does not pollute the technological effluents, i.e. reduces the load on the treatment plant.

In this case, the drug is obtained in the form of a stable complex with an increased shelf life, increased bioavailability and digestibility of iodine, prolonged action and with an expanded range of therapeutic and functional (functional) capabilities.

Thus, as a result of the invention, an efficient and inexpensive method for the production of homogenized brown algae in the form of a biologically active preparation of a wide spectrum of activity was created, as well as an expanded arsenal of methods for the production of homogenized brown algae in the form of biologically active preparations

At the same time, minimization of the loss of biologically active substances and the preparation of the drug in the form of a stable complex with a high content of biologically active easily digestible substances with a molecular weight of 70 Da to 100 kDa: oligo- and monosaccharides (mainly mannitol), amino acids and peptides, nucleotides and trace elements in the form of biogenic compounds, mainly with proteins and peptides, increased shelf life, increased bioavailability and digestibility of iodine, prolonged action and with an extended range Sohn therapeutic and prophylactic (functional) features including increasing the body's resistance in diseases of the gastrointestinal tract, cardiovascular and endocrine systems, as well as antnoksndantnoe and immunomodulatory effects, with improved organoleptncheskimi characteristics and minimal probability of side effects. The active ingredients of the gel have an anti-sclerotic, hepatoprotective effect, improve metabolic processes in the liver, increasing its resistance to adverse conditions; increase the activity of the enzymatic systems of the liver; accelerate the regeneration of liver cells after damage, toxic effects and infectious diseases. They interact with free radicals in the liver and translate them into less toxic compounds, interrupting the process of lipid peroxidation, and prevent further destruction of cellular structures. In damaged hepatocytes, the synthesis of structural and functional proteins and phospholipids is stimulated, and cell membranes are stabilized.

Information sources

1. RU №2000126420, 2002

2. RU No. 2108108, 2000

3. RU No. 2343724, 2009 (prototype).

Claims (12)

1. A method for the production of biologically active products from brown algae, involving the cutting of raw materials, in which thalli are taken from fresh brown algae, rhizoids are cut, washed with sea water from salt and solids and dried, and then washed in fresh water at a temperature of 25-35 ° C for 20-50 minutes with periodic stirring for uniform swelling, not less than 30%, then algae are demineralized, for which they are poured with a 0.1-2% solution of at least one food acid from the group of citric acid , u velvety, phosphoric, acetic, dairy, succinic, hydrochloric or sulfuric, having an acidity of pH 2.0, with a mass ratio of algae and solution equal to 1: 1.5, and incubated for 0.5-10 hours at a temperature of 10-50 ° C, after which the food acid solution in which demineralization was carried out is filtered, desalted and separated, and the algae are washed from the acid in fresh water for 10-12 minutes and soaked in drinking water at a temperature of 20-35 ° C for 30- 50 min, allow water to drain for 10-20 min, washed algae is crushed and rubbed with odes from the group: cranberries, prunes, dried apricots, lingonberries, sea buckthorn, rosehips, milk thistle, in an amount up to 20% by weight of algae, and placed in a cooking container with a mixing device and a homogenizer, add at least a portion of the filtered and desalted solution food acid, in which demineralization was carried out, drinking water with a temperature of 75-85 ° C and at least one food alkali from the group: calcium carbonate, sodium bicarbonate, sodium hydroxide, potassium hydroxide, magnesium carbonate, magnesium hydroxide until reaching acidity pH from 7 to 12, which is controlled during the cooking process, carried out for 0.5-18 hours at a temperature of 40-100 ° C with continuous stirring with a stirrer at a speed of 15-25 rpm, and then cooled to 35- 45 ° C and homogenized by the action of centrifugal forces of the rotor with the blades together with hydroacoustic and hydrodynamic vibrations for 30 minutes, then they are neutralized, for which solutions of citric acid are introduced in an amount of 0.1% and edible calcium salt in an amount of 0.2% by weight seaweed, mix thoroughly in t for 10 minutes, heated to 75 ° C and converted into a form suitable for storage with a content of 50-99% water and at least 30% polysaccharides in terms of dry matter. 2. The method according to claim 1, characterized in that the solution of food acid, which was demineralized, is filtered and desalted by dialysis on either semipermeable or diffusion or osmotic membranes with a selectivity of 50-1500 Da. 3. The method according to any one of claims 1 and 2, characterized in that as brown algae take algae from the group: kelp and fucus from the basin of the Sea of Okhotsk, the Bering Sea or the Sea of Japan. 4. The method according to any one of claims 1 and 2, characterized in that the rhizoids are cut manually or on circular knives, and the thalli are washed with sea water in a cascade or vortex washing or by showering. 5. The method according to any one of claims 1 and 2, characterized in that in the case of spirorbis on the surface of the thalli, they are cleaned with brushes. 6. The method according to any one of claims 1 and 2, characterized in that the dry algae is washed in fresh water by showering in a bath, washing off sand and salt. 7. The method according to any one of claims 1 and 2, characterized in that for washing use natural drinking water with a hardness of up to 1 mol / m ³ or softened (corrected) drinking water with a pH of not more than 7.8 and hardness of up to 0, 2 mol / m, washed algae is crushed in a meat grinder or pulper. 8. The method according to any one of claims 1 and 2, characterized in that for cooking the washed algae is crushed and filled with hot water in a cooking vessel with a ratio of supplied heating power and net volume equal to 0.20-0.25 kW / l, for example , in a reactor with a thermostatic jacket and a stirrer, with a pit-hole for heating with dry steam, or in an electric boiler with a stirrer, and homogenized in a built-in or remote homogenizer for 30 min by the action of hydroacoustic and hydrodynamic vibrations. 9. The method according to any one of claims 1 and 2, characterized in that during neutralization flavor additives are added to the mass, and immediately before cooking, stevioside is added in an amount of up to 1 mg per 1% of the mass of algae. 10. The method according to any one of claims 1 and 2, characterized in that before converting to a suitable storage form, the gel is enriched with polyvalent metal salts of the group: magnesium chloride, magnesium sulfate, calcium chloride, calcium citrate, calcium lactate based obtaining a final concentration of 0.001-1% in a gel, which is controlled for iodine content, which should be at least 15 mg / kg, carbohydrate fractions, which should be: mannitol - (245.4 ± 12.1) mg / g, alginic acid - (186.1 ± 10.7) mg / g. 11. The method according to any one of claims 1 and 2, characterized in that before converting into a form suitable for storage, check the hygienic characteristics in terms of the content of toxic elements: lead - not more than 0.5 mg / kg, arsenic - not more than 0 5 mg / kg, cadmium - not more than 1.0 mg / kg, mercury - not more than 0.1 mg / kg, DDT - not more than 2.0 mg / kg, radionuclides - not more than 6 Bq / kg. 12. The method according to claim 10, characterized in that the gel is Packed in hot form or pre-dried to convert to a form suitable for storage, and drying is carried out using a freeze-drying dryer or a vacuum drying unit or a spray drying unit convection drying, while the prepared pasteurized mass is poured on baking sheets or in ditches with a layer of not more than 10 mm, which are placed on shelves in a drying unit, where the mass is dried to a given final moisture content.