RU2323600C2 - Method of brown algae gel production for dietary and prophylactic feeding - Google Patents

Abstract

FIELD: food industry.

SUBSTANCE: method of brown algae gel production provides for cutting raw material and washing with sea water. Then it is washed in fresh water at the temperature 25-35°C for 20-50 minutes for even swelling not less than 30%. After that the algae is demineralized by 0.5% acid solution: acetic, citric or hydrochloric, рН=2.0, with the weight ratio of the algae and solution - 1:1.5 within 1-2 hours. The algae is washed in fresh water for 10 minutes and is soaked in fresh water at the temperature 20-35°C for 30-50 minutes and change the water 1-2 times. Let the water flow down for 10-20 minutes. The algae is cut and boiled in fresh water at the temperature 75-85°C with the algae and water ratio 1:1. Then add sodium bicarbonate till рН=7.0-8.0, which is controlled while boiling for 6-10 hours at the temperature 70-80°C and mix with agitator, rotation speed is 15-25 revolutions per minute. Cool till 35-45°C and homogenize within 30 minutes. Introduce citric acid solution - 0.1% and calcium salt solution- 0.2%. Mix for 10 minutes, heat till 75°C and transform into shape suitable for storage.

EFFECT: increases storage life, increased biological accessibility and iodine digestibility, prolonged action.

12 cl, 4 ex

Description

The invention relates to the production of food product "Brown homogenized algae (kelp and fucus) for dietary and preventive nutrition" produced from varieties of kelp (seaweed) and / or fucus.

Laminaria is one of the most common representatives of brown algae. It lives in the Far Eastern and Northern seas. The algae thallus attaches to the substrate with the help of rhizoids. A cylindrical stem (up to 50 cm long) departs from the rhizoids, it passes into a solid or dissected plate 4-5 m long. Fucus is another widespread representative of brown algae. It is a branchy bush attached to a substrate by a disk (rhizoid). The branches are belt-shaped, on some there are air-filled cavities filled with air and serving to maintain the plant in an upright position.

Brown algae contain substances necessary for the human body, and are used in the preparation of various biologically active preparations.

Known drug obtained by preliminary destruction of brown algae kelp with its subsequent extraction and the addition of components provided for by the formulation [1].

The disadvantage of this drug is its instability, poor biological activity, the presence of important contraindications and low attractiveness for organoleptic indicators.

Known drug containing kelp or its concentrate, garlic, sodium alginate and beckon. The starting components are thoroughly mixed in the claimed proportions and subjected to granulation by the conventional method, garnul is dried at t not higher than 100 ° C, the target product is obtained in the form of granules 2 mm in size, granules are packaged in 0.3 g capsules [2].

The disadvantages of this drug are poor storage stability, i.e. short (no more than 30 days) shelf life of the product, weak biological activity and the narrow range of functional preventive capabilities due to non-optimal cooking process, in which a sufficient amount of iodine-organic complexes and calcium alginates are not formed, the composition and ratio of ingredients, short duration of action, low attractiveness organoleptic indicators (unpleasant aftertaste). An important disadvantage of this drug is the low digestibility of its iodine. For example, in the Far Eastern regions of Russia, the population who consume seafood in abundance, including seaweed (kelp), is prone to diseases characteristic of iodine deficiency almost to the same extent as in the European part. This is due to the fact that the intake of products containing iodine in its natural form is not effective enough. Such a drug, containing mainly iodine in its natural form, is contraindicated in nephritis, hemorrhagic diathesis, and other conditions in which iodine preparations are undesirable.

An object of the invention is the creation of an effective and inexpensive biologically active drug with a wide spectrum of action, as well as the expansion of the arsenal of biologically active drugs.

The technical result, which provides a solution to the problem, is to obtain the drug in the form of a stable complex with an increased shelf life, increased bioavailability and digestibility of iodine, prolonged action and with an expanded range of preventive (functional) capabilities, including increasing the body's resistance to diseases of the gastrointestinal tract , cardiovascular and endocrine systems, as well as antioxidant and immunomodulatory effects with improved organoleptic characteristics and the minimum probability of side effects.

The essence of the invention lies in the fact that the method of producing a gel from brown algae involves cutting raw materials, in which thalli are taken from fresh brown algae, cut rhizoids, washed with sea water and dried, and then washed in fresh water at a temperature of 25-35 ° C for 20-50 minutes with periodic stirring for uniform swelling of at least 30%, then algae is demineralized, for which they are poured with a 0.5% solution of an acid from the group: acetic, citric or hydrochloric acid having an acidity of pH 2.0, at the mass ratio of algae and solution equal to 1: 1.5, and incubated for 1-2 hours, after which they are washed from acid in fresh water for 10-12 minutes and soaked in drinking water at a temperature of 20-35 ° C for 30-50 minutes with a change of water during the soaking process, and after the last washing, let the water drain for 10-20 minutes, the washed algae is crushed and boiled, for which it is poured with drinking water at a temperature of 75-85 ° C with a mass ratio of algae to water equal to 1: 1, sodium bicarbonate is added until acidic pH 7.0-8.0, which is controlled during the cooking process, carried out for 6-10 hours at a temperature of 70-80 ° C with continuous stirring with a stirrer with a rotation speed of 15-25 rpm, after which it is cooled to 35- 45 ° C and homogenized for 30 min by the influence of hydroacoustic and hydrodynamic vibrations, then they are neutralized, for which solutions of citric acid in an amount of 0.1% and edible calcium salt in an amount of 0.2% by weight of algae are introduced, mix thoroughly for 10 minutes, heated to 75 ° C and converted into a form suitable for storage.

At the same time, algae from the group: kelp and fucus from the basin of the Sea of Okhotsk, the Bering or Sea of Japan are taken as brown algae, a solution of calcium lactate or calcium citrate is taken as a solution of edible calcium. Preferably, the rhizoids are cut manually or on circular knives, the thalli are washed with sea water in a cascade or vortex washing or by scrubbing, if spirorbis is present on the surface of the thalli, they are brushed, the dried algae are washed in fresh water by scrubbing in the bath, washing off sand and salt , are used for washing natural drinking water with a hardness of up to 1 mol / m ³ or softened (amended) drinking water having a pH of not more than 7.8 and rigidity to 0.2 mol / m ^3, the washed seaweed is milled on myasor BKE or a cleaning machine. In addition, for cooking, washed algae is crushed and filled with hot water in a cooking vessel with a ratio of supplied heating power and usable volume equal to 0.20-0.25 kW / l, for example, in a reactor with a thermostatic jacket and a stirrer having a cutting edge for heating with dry steam, or in an electric boiler with a stirrer, flavorings are added to the mass during neutralization, and after reaching a pH value of 7.0-8.0, immediately before cooking, add steviazide in an amount of 1 mg per% of the mass of algae, By conversion to a form suitable for storage, the iodine content, which should be at least 15 mg / kg, carbohydrate fractions, which should be: mannate - 133.4 ± 12.1 mg / g, alginic acid - 147 is controlled in the resulting gel , 8 ± 10.7 mg / g, and also check the hygienic characteristics regarding the content of toxic elements: lead - not more than 0.5 mg / kg, arsenic - not more than 0.5 mg / kg, cadmium - not more than 1.0 mg / kg, mercury - not more than 0.1 mg / kg, DCT - not more than 2.0 mg / kg, radionuclides - not more than 6 kg / kg. In particular, to convert it into a form suitable for storage, the gel is packaged hot or pre-dried, moreover, the drying is carried out using a freeze dryer, or a vacuum dryer, or a spray dryer, or a convection dryer, the pasteurized mass is poured onto baking sheets or in ditches with a layer of not more than 10 mm, which are placed on shelves in a drying unit, where the mass is dried to a final moisture content of not more than 10%.

The method is implemented as follows.

When implementing the method, the following ingredients are used:

- kelp according to GOST 20438 (or raw sea cabbage according to TU 15-01-360);

- food fucuses according to OST 64-026-87;

- acetic acid according to GOST 61-75;

- food citric acid according to GOST 908-2004;

- hydrochloric acid according to GOST 3118 or according to GOST 3857;

- calcium citrate according to TU 6-09-01-263-85;

- calcium lactate according to TU 6-09-3839;

- steviazide according to current regulatory and technical documentation or obtained by import;

- sodium bicarbonate (sodium bicarbonate) according to GOST 4201;

- drinking water according to GOST R 51232 with hardness up to 1 mol / m ³ or softened (corrected) drinking water with a pH of not more than 7.8 and hardness up to 0.2 mol / m ³ .

At the same time, sodium bicarbonate (sodium bicarbonate) is an alkalizing agent, odorless, white crystalline powder. The chemical formula is NaHCO ₃ .

Physico-chemical characteristics of sodium bicarbonate Mass fraction of sodium bicarbonate,%, not less than 99.5 Mass fraction of sodium carbonate,%, no more 0.4 Mass fraction of chlorides in terms of NaCl,%, no more 0.02 Mass fraction of iron (Fe),%, no more 0.001 Mass fraction of calcium (Ca),%, no more 0.04 Mass fraction of sulfate in terms of SO ^2- _4%, no more 0.02 Mass fraction of moisture,%, no more 0.1

Sodium bicarbonate is an antacid. When taken orally, it interacts with HCl of gastric juice, neutralizing it. Increases excretion of Na + and Cl-, osmotic diuresis; shifting the reaction of bronchial mucus to the alkaline side, increases bronchial secretion, reduces the viscosity of sputum and improves its expectoration. Eliminates pain in peptic ulcer and 12 duodenal ulcer. Alkalization of the urine prevents the precipitation of uric acid in the urinary tract. In its pure form, as a result of the neutralization reaction, CO ₂ can cause discomfort in the stomach and burping.

Calcium citrate (calcium citrate) (E331), chemical formula [OOCS (OH) (CH ₂ COO) ₂ ] ₂ Ca ₃ * 4H ₂ O - calcium tetrahydrate citrate - calcium preparation, approved and recommended for use in the food industry. The products are accompanied by the Sanitary and Epidemiological Report of the Ministry of Health of the Russian Federation.

It has been established that calcium ions are involved in the excitation and contraction of muscle cells, ensuring cell permeability, intercellular interactions, blood coagulation, secretion of hormones, mediators, and enzymes. Calcium ions act as a transformer of information that enters the cell, regulate intracellular metabolism, including energy. From the extracellular space they penetrate into the protoplasm through special calcium channels, affecting various physiological processes, the functions of the cells of all organs, vascular tone, systole intensity, diastole. Calcium ions play an important role in the formation and preservation of bone tissue. Calcium citrate replenishes the lack of calcium ions in the body with functional (physiological increased need as a result of growth, normal blood loss in women, leaching of bone calcium in elderly people, etc.) and pathological (trauma, bleeding, malabsorption in the gastrointestinal tract , inability to reabsorb calcium in the kidneys, etc.) insufficiency of replenishment of the body with calcium ions. When using the drug in its pure form, loss of appetite and abdominal pain may occur.

Calcium lactate is a calcium preparation approved and recommended for use in the food industry. The products are accompanied by the Sanitary and Epidemiological Report of the Ministry of Health of the Russian Federation.

Calcium lactate regulates calcium-phosphorus metabolism, has anti-inflammatory, anti-rheumatic, vitamin and hemostatic effects. It accelerates all phases of blood coagulation, increases platelet adhesion, and promotes the rapid entry of tissue thromboplastin into the blood. When using the drug, heartburn can occur.

Stevioside - a natural sweetener - Stevia honey herb extract (Paraguay). Stevioside - approximately 200-300 times sweeter than sugar, calorie-free, does not cause caries. Stevioside improves the functioning of the cardiovascular, immune and other systems; thyroid gland, liver, kidneys, spleen. It normalizes blood pressure, has antioxidant, adult, anti-inflammatory, anti-allergenic and moderate choleretic effect. It inhibits the development of many microorganisms and is therefore recommended for diseases of the oral cavity: it protects teeth from caries, and gums from periodontosis, which is a common cause of tooth loss, including diabetes mellitus, helps to prevent bad breath.

In the manufacturing process, the following processing steps are performed.

1. The preparation of algae.

As brown algae take algae from the group: kelp and fucus from the basin of the Sea of Okhotsk, Japan or the Bering Sea outside the zones of industrial and household waste. The production of algae is carried out in accordance with Instruction No. 6, approved by order of "DALRYBA" No. 466 of July 10, 1985. Algae are used - raw or dried, which are allowed for use in the food industry of the SSES of the RF:

2. Cutting

Fresh brown algae take thalli (sea stalk), cut rhizoids (growths) manually or on circular knives. Produce unfit plants.

3. Washing

Thalli are washed with sea water in a cascade or vortex washing or by showering. If there are spiroorbis thalli (shells) on the surface, they are cleaned by hand with brushes, followed by washing.

After washing, the thalli are placed on a mesh tray for draining water and drying. Dry algae are washed in fresh water in the bath by the method of overflowing, washing off sand and salt. After that, at a temperature of 25-35 ° C give exposure for 20-50 minutes with periodic stirring for uniform swelling, at least 30%.

4 Demineralization

Algae is poured with a 0.5% solution of acetic, or citric, or hydrochloric acid (pH 2.0) at a mass ratio of algae: solution equal to 1: 1.5, and incubated for 1-2 hours.

5. Flushing

After the specified time, algae are washed from acid in fresh water by strangulation for 10 minutes and soaked in a hopper in drinking water at a temperature of 20-35 ° C for 30-50 minutes with 1-2 times the change of water during soaking.

After the last washing, the container with algae is lifted from the hopper and allowed to drain water for 10-20 minutes.

6. Shredding

The washed algae is ground in a meat grinder M-250, MIM-300, MIM-600, TL2N (made in China) or MPR-350.01 wiping machine and loaded into a cooking container.

7. Cooking

The crushed algae is loaded into a cooking vessel with a ratio of supplied heating power and usable volume equal to 0.040-0.045 kW / l, for example, a reactor with a volume of 1050 l, having electric heaters with a power of 45 kW and made with a cutting room for heating with dry steam, or in an electric boiler with a stirrer (with a similar ratio of power of electric heaters and volume) and with a thermostatic jacket. Hot potable water with a temperature of 75-85 ° C is poured into the reactor in proportion to the algae mass of 1: 1, sodium bicarbonate is added until a pH of 7.0-8.0 is obtained, which is controlled during the cooking process, and stevioside up to 1 mg per percent masses. Cooking is carried out for 6-10 hours at a temperature of 70-80 ° C with continuous stirring with a stirrer with a rotation speed of 15-25 rpm.

8. Homogenization

After cooking, the resulting mass is cooled to 35-45 ° C and homogenized for 30 minutes, for example, with a RPA homogenizer. Rotary pulsation apparatuses (RPA) combine the principles of operation of a hydrodynamic oscillation generator and a centrifugal pump. By means of pulsating, shock and hydrodynamic effects, by the action of centrifugal forces, together with the hydroacoustic and hydrodynamic vibrations occurring in RPA, the physicomechanical properties of the product are changed, which is converted into the most effective and convenient form for the body to absorb. In the process of homogenization, the particles are crushed to about one micron, uniformly distributed in the mass of the product. Under the influence of pulsation, shock and hydrodynamic effects, together with hydroacoustic and hydrodynamic vibrations, processes of dispersion, emulsification, dissolution, extraction, oxidation, polymerization are carried out. At the same time, intense acoustic exposure leads to the destruction of bacteria and microorganisms at this stage. Released substances of peptide nature are able to regulate the various stages of proliferation and differentiation of T-lymphocytes and lymphocytes, that is, act on the links of the immune response. The homogenized product does not exfoliate during prolonged storage, suitable for transportation, while maintaining its physiological qualities.

9. Neutralization

Solutions of citric acid in an amount of 0.1% by weight of algae and a 15-25% solution of edible calcium salt in an amount of 0.2% by weight of algae are introduced into the homogenized mass. As a solution of edible calcium salt, a solution of calcium lactate or calcium citrate is introduced. At this stage, flavoring additives can be introduced (according to a specific formulation, for example, lemon balm or caraway seeds in an amount of 0.05% by weight of algae). The mass is thoroughly mixed for 10 minutes. During processing, the release of alginic acid, which is an intercellular substance and one of the components of the cell walls of algae, occurs. The introduced substances form complex compounds with algae homogenization products, mainly sodium-calcium alginates. Most of the iodine is in the form of organic iodine compounds. The synergistic effect of the ingredients in optimally selected active doses serves to prevent secondary immunodeficiency resulting from the effects of stress, vaccination, radiation or chemotherapeutic treatment, transplacental transmission of the influenza virus to the fetus, as well as age-related changes in the immune systems that are the factors of emerging diseases.

After that, the mass is heated to 75 ° C (pasteurized) and converted into a form suitable for storage, for example, packed in hot form or served for drying.

10. Drying

For drying, use the freeze-drying unit P1-ISS-101, a unit of type TG-15 or TG-50 (Germany) or vacuum drying SPT-200, spray drying "Niro atomizer" or convection drying IP-120 (Hungary).

Prepared pasteurized mass is poured on baking sheets or in ditches with a layer of not more than 10 mm, which are placed on shelves in a drying unit, where the mass is dried. The final moisture content of the product should not exceed 10%.

11. Packing

11.1. Dry packaging

The finished powder of the drug is packaged in heat-sealed film bags according to TU 15-1106-90 with a maximum weight of 100 g or in other containers made of materials allowed for contact with foodstuffs by the State Sanitary and Epidemiological Supervision of the Russian Federation and ensuring the safety of products.

11.2. Hot packing

The mass is Packed in hot form with a dispenser UD-2 or UFM in sealed containers and cooled.

The resulting gel contains 92-94% water, 6-8% solids, which include alginic acid 5-6% in the form of sodium calcium alginate, fiber - 1-1.5%, protein - 1%, mineral micro and macronutrients: molybdenum (12 × 10 - 3%), manganese (4.4 × 10 - 3%), iron (13 × 10 - 3%), fucoidan, stevioside and others. Nitrogenous substances are represented by 17 amino acids, among which 7 are essential. The resulting product is an elastic sliding texture, pleasant sweet and sour taste and with a tonic aroma with notes of jasmine and mountain herbs. Preservation (stability) of properties was controlled during 6-8 months of storage at a temperature of 8-12 ° C in appearance - preservation of taste, color, consistency and smell, the absence of delamination, swelling, gas formation. No changes in these indicators during storage were found.

The following are examples of the practical use of the gel obtained by the claimed method.

Example 1

The study of the effects of the gel obtained by the claimed method in diseases of the gastrointestinal tract was carried out on the following groups of patients: patients of the experimental group - 32 people (28 men, 4 women) with an average age of 55 ± 7.8 years. The control group was 28 people aged 55 ± 5.6 years.

Patients complained of nausea, belching, heartburn, epigastric pain and a feeling of heaviness in the right hypochondrium, an intestinal upset in the form of diarrhea or constipation. In the study of laboratory parameters in a number of patients revealed an increase in the level of ACT, ALT, LDH and bilirubin due to the indirect fraction. The coprogram has an increased amount of fatty acids.

Coprogram indicators before and after consuming brown algae gel (in% of the total number of cases) No. Indicators no does not mean. moderately much before after before after before after before after one. Muscle fibers 0 0 57 53 36 40 7 7 2. Neutral fat 68 76 22 16 9 9 0 0 3. Fatty acid twenty eleven 42 40 22 22 16 27 four. Soaps 51 53 27 22 twenty 24 2 0 5. Indigestible fiber 29th 7 38 33 24 53 9 7 6. Digestible fiber 44 31 31 44 16 eleven 9 13 7. Starch 16 22 53 53 twenty eleven eleven 13

The experimental group for 21 days, along with generally accepted in similar cases, medicines received 50 ± 5 g of gel produced by the claimed method, 30 minutes before meals in the morning and evening. Of these, 15 people received a product for the preparation of which only kelp was used, and calcium edible salt — calcium lactate, the other 17 people — a product for the preparation of which kelp and fucus were used in a mass ratio of 1: 1, and as a food salt of calcium - calcium citrate, in this batch, stevioside was introduced into the composition of the product. The shelf life of these products was 6 months. The control group received only standard drug treatment and the well-known drug containing kelp, Lamisan-L.

Patients of the experimental group, starting from the 5-7th day of the course, noted a steady improvement in overall health: normalization of appetite, decreased heartburn, belching, nausea, epigastric pain and heaviness in the right hypochondrium, as well as normalization of stool. Subjective improvement was confirmed by the positive dynamics of objective indicators in the experimental group. In 80% of patients, palpation sharply decreased or completely absent pain in the epigastrium and in the area of the projection of the duodenal bulb, as well as along the intestine and in the right hypochondrium. In the control group, the positive dynamics were less pronounced, the improvement in the patients' well-being came, as a rule, at a longer time (later by 10-18 days). Such signs as a feeling of heaviness in the right hypochondrium, skin itching, leg pain, dyspeptic symptoms, in the experimental group were stopped in a shorter time period than in patients of the control group.

In the biochemical analysis of the patients of the experimental group receiving both product variants, the total protein and bilirubin content had a clear tendency to normalize. In the control group, positive changes were less pronounced and occurred at a longer time. Girdle pains, dyspeptic symptoms, in patients of the experimental group were stopped earlier than in patients of the experimental group. None of the patients noted unpleasant sensations in the stomach and belching, unpleasant aftertaste or other unpleasant phenomena.

In patients of the experimental group who received any of the mentioned product options, an improvement in the ultrasound picture of the liver was noted by 18-21 days of the course. In the control group, the ultrasound data at this moment remained without significant changes. Over the next 3-4 months, most patients did not have complaints characteristic of the moment of the start of the course of taking the gel obtained by the claimed method.

Example 2

The study of the antibacterial properties of the gel obtained by the claimed method was carried out on 32 patients who were divided into two groups: the main - 16 people and the control group - 16 people. The average age of patients (in both groups) was 52 + 1.6 years. These groups were identical in age and disease standard in accordance with ICD-10.

At the beginning of the course, an endoscopic examination for chronic constipation revealed bacterial-induced structural changes in the colon mucosa, which were expressed in focal inflammatory (49.2%) changes, as well as the presence of erosion on the colon mucosa (31.1%). Morphological changes in the colon mucosa were found in all patients. There were violations of the ratio of opportunistic and saprophytic intestinal microflora, opportunistic microflora were seeded in 80.2% of patients. In patients of the control and experimental groups, the analysis of feces for dysbiosis revealed the presence of Staphylococcus aureus and an increased titer of E. coli. Two of the experimental group suffered from nephritis, one - hemorrhagic diathesis.

The experimental group received 28 ± 5 g of gel 30 minutes before meals in the morning and evening, along with standard medicines in similar cases, for 28 days. The control group received only standard drug treatment. Of these, 8 people received a product with a shelf life of 6 months, for the preparation of which only kelp was used, and calcium citrate was used as calcium salt, in this batch stevioside was introduced into the composition of the batch, and 9 people received a product with a shelf life of 6.5 months, for the preparation of which kelp and fucus were used in a mass ratio of 1: 2, and calcium lactate as a dietary salt of calcium.

Laboratory and instrumental methods of treatment and examination were carried out in accordance with the standards.

According to endoscopic and morphological studies during the course in patients of the experimental group, a positive dynamics of changes in the colon mucosa was established, which was expressed in a decrease in inflammatory (89.4%) and elimination of erosive changes in the colon mucosa (100%), reduction in cell infiltration ( 74.8%) of patients. Under the influence of a brown algae gel, chemical changes in feces normalized: pH normalized in 98.3% of patients, organic acids and ammonia normalized in 98.5%, mucin remained in feces only in 4.7% of patients, tissue protein was not noted. In all patients of the experimental group, scarring of the ulcer defect occurred in a shorter time than in the control group, cicatricial deformity was less pronounced. The positive dynamics of the controlled parameters of patients suffering from nephritis and diathesis did not practically differ from the dynamics of other patients; no deterioration of other indicators was found. Patients noted a pleasant taste and smell of the product, the absence of an unpleasant aftertaste. Over the next 4-5 months, the majority of patients did not show signs of dysbacteriosis and exacerbation of diseases characteristic of the moment of the start of the course of taking the gel obtained by the claimed method.

Example 3

Evaluation of the effectiveness of treatment of iodine deficiency was carried out in two groups of pregnant women. Group 1 - an experimental of 66 people, Group 2 - a control of 52 people.

The dynamics of the content of blood iodides (μmol / l) in pregnant women after taking the gel from brown seaweed Examination Period (weeks) 8-12 (initial) 16-24 28-32 34-38 1 group 12.67 ± 3.44 18.73 ± 4.99 19.2 ± 2.52 20.64 ± 3.44 2 group 12.87 ± 2.56 13.07 ± 2.58 13.66 ± 4.72 14.95 ± 1.79

Significant differences were found in the content of blood iodides before and after treatment of iodine deficiency in women. The first group included 86 pregnant women who received complex treatment corresponding to the form of the anemic condition, and an additional iodine-containing therapeutic and prophylactic product — a brown seaweed gel of 50 ± 5 g of gel 30 minutes before meals in the morning and evening for 12 days before each control research. Of these, 36 women received a product with a shelf life of 5.5 months, for the preparation of which only kelp was used, and calcium citrate was used as calcium salt, in this batch, stevioside was introduced into the product, and another 26 women received the product, shelf life which was 6 months, for the preparation of which only fucus was used, and calcium lactate as calcium salt, in this batch, stevioside was introduced into the composition of the product, the remaining 24 women - a product whose shelf life was 6 months, d I whose preparation Fucus and Laminaria used in a weight ratio of 2: 1, and as a dietary calcium salt - calcium citrate.

The second group consisted of 65 pregnant women, also received complex treatment depending on anemia, and one of the preparations containing kelp - Laminarid or Lamisan-L (aqueous kelp concentrate), almost equivalent to the prototype. The control was carried out by determining the activity of iodides in whole blood by direct potentiometry using ion-selective electrodes of the company "SguSh" (Czech Republic). Normally, these indicators are 20-50 μmol / L. Initially, a comprehensive clinical and laboratory study was carried out when registering in a antenatal clinic in the early stages. Women who had up to 12 weeks of gestation with a blood iodide content of 12.32 ± 2.23 μmol / L were selected in the experimental and control groups. During the course, comprehensive clinical and laboratory studies were carried out at 16-24, 28-32, 34-38 weeks of pregnancy. In the first group, upon receipt of the product - gel from brown seaweed, an increase in the content of blood iodides to values near the lower limit of the norm is noted. In women who received a well-known product, only a slight improvement was recorded, which at the end of the course remained significantly below normal, i.e. iodine deficiency was compensated slightly. None of the patients receiving the product noted an unpleasant aftertaste, discomfort in the stomach, loss of appetite, or abdominal pain. The improvement in the experimental group for all women who received any of the product options was stable and remained between the courses of taking the product.

Example 4

Assessment of the effectiveness of treatment in patients with cardiovascular diseases was carried out in two groups. 1 group of 34 people (men and women) - experimental, 2 group of 30 people (men and women) - control.

The first group received complex treatment and a prophylactic product for 45 days — a brown algae gel obtained by the claimed method, 50 ± 5 g of gel 30 minutes before meals in the morning and evening. Of these, 16 people received a product whose shelf life was 6 months, for the preparation of which only kelp was used, and calcium citrate as calcium salt, in this batch, stevioside was introduced into the composition of the product, the remaining 18 people received a product whose storage period was 6.5 months, for the preparation of which kelp and fucus were used in a mass ratio of 2: 1, and calcium lactate as a dietary salt of calcium.

The second group received complex treatment and one of the well-known drugs containing kelp - Laminarid or Lamisan-L.

Changes in lipid and carbohydrate metabolism, blood pressure and prothrombin index in patients with cardiovascular diseases under the influence of oral administration of a gel from brown seaweed Indicators 1st group (15 people) 2nd group (15 people) Before treatment After treatment Before treatment After treatment Triglycerides, mmol / L 1.5 ± 0.11 1.2 ± 10.10 1.76 ± 0.87 1.68 ± 0.74 Total cholesterol, mmol / l 6.09 ± 0.2 5.13 ± 0.32 7.2 ± 0.30 6.84 ± 0.31 / 3-lipoproteins, g / l 6.61 ± 0.20 6.1 ± 0.19 7.41 ± 1.25 7.28 ± 1.65 a-cholesterol, mmol / l 1.51 ± 0.10 1.82 ± 0.06 1.50 ± 0.12 1.61 ± 0.12 / 3-cholesterol, mmol / l 3.51 ± 0.27 2.72 ± 0.13 5.22 ± 0.27 4.99 ± 0.26 Atherogenic coefficient 2.2 ± 0.09 1.85 ± 0.09 Fasting blood sugar, mmol / L 6.3 ± 0.52 5.01 ± 0.17 6.1 ± 0.14 5.88 ± 0.07 Body weight kg 94.3 ± 0.52 92.5 ± 0.29 96.2 ± 0.61 95.5 ± 0.52 HELL systole., Mm Hg 143.7 ± 2.1 130.0 ± 2.1 147.5 ± 1.8 144.2 ± 2.0 HELL diastole., Mm Hg 88.0 ± 2.1 80.6 ± 1.4 97.0 ± 1.5 96.8 ± 2.1 Prothrombin index,% 97.5 ± 2.6 86.71 ± 0.17 103.8 ± 0.72 102.7 ± 0.4

The results shown in the table demonstrate a more effective normalizing effect of the drug obtained by the claimed method on the pathogenetic links of cardiovascular diseases that has occurred in patients receiving any of the product variants. Control tests taken 3 and 6 months after the completion of the course showed that these indicators did not change compared to the moment the course was completed. Patients noted a pleasant taste and smell of the product, the absence of an unpleasant aftertaste or dyspeptic symptoms.

From the above examples and other studies, one can see the healing properties of various components of the gel from brown seaweed obtained by the claimed method, as well as its ability in regenerative medicine as a means of general healing of the body and preserving the health reserve, as well as a product used in the complex treatment of diseases. The negative consequences of taking the drug were not identified, i.e. side effects (which are the basis for establishing contraindications) were not detected. The result of taking the drug is stored for a period of at least three times the duration of the course of administration. Confirmed by tests at a temperature of 6-10 ° C, the shelf life of the properties is 6 months.

The brown algae gel obtained by the claimed method is one of the most modern and high-quality biologically active products intended for independent, complex and auxiliary therapy, maintaining human health and the complex treatment of a wide range of diseases of the gastrointestinal tract, cardiovascular and endocrine systems , has antioxidant and immunomodulatory effects, as well as improved organoleptic characteristics and a minimal likelihood of side effects oi.

Thus, an effective and inexpensive biologically active drug of a wide spectrum of action was created, as well as an expanded arsenal of biologically active drugs.

At the same time, the drug was obtained in the form of a stable complex with an increased shelf life, increased bioavailability and digestibility of iodine, prolonged action and with an expanded range of therapeutic and prophylactic (functional) capabilities, including increasing the body's resistance to diseases of the gastrointestinal tract, cardiovascular and endocrine systems, as well as antioxidant and immunomodulatory effects with improved organoleptic characteristics and minimal chance of side effects Nij.

Information sources

1. RU No. 2000126420, 2002

2. RU No. 2108108, 2000 (prototype).

Claims (12)

1. A method of producing a gel from brown algae, involving the cutting of raw materials, in which thalli are taken from fresh brown algae, rhizoids are cut, washed with sea water and dried, and then washed in fresh water at a temperature of 25-35 ° C for 20-50 minutes with periodic stirring for uniform swelling by at least 30%, then the algae is demineralized, for which they are poured with a 0.5% solution of the acid from the group: acetic, citric or hydrochloric acid having an acidity of pH 2.0, with a mass ratio of algae and a solution equal to 1: 1.5, and incubated for 1-2 hours, after which they are washed from acid in fresh water for 10-12 minutes and soaked in drinking water at a temperature of 20-35 ° C for 30-50 min with a change of water during the soaking process, and after the last washing, let the water drain for 10-20 minutes, the washed algae is crushed and cooked, for which it is poured with drinking water at a temperature of 75-85 ° C with a mass ratio of algae to water equal to 1: 1, sodium bicarbonate is added until the pH value is 7.0-8.0, which is controlled during f cooking, carried out for 6-10 hours at a temperature of 70-80 ° C with continuous stirring with a stirrer with a rotation speed of 15-25 rpm, after which it is cooled to 35-45 ° C and homogenized for 30 minutes by the action of hydroacoustic and hydrodynamic vibrations, then neutralize, for which solutions of citric acid are introduced in an amount of 0.1% and edible calcium salt in an amount of 0.2% by weight of algae, mixed thoroughly for 10 minutes, heated to 75 ° C and converted into a form suitable for storage. 2. The method according to claim 1, characterized in that as brown algae take algae from the group: kelp and fucus from the basin of the Okhotsk, Bering or Sea of Japan. 3. The method according to any one of claims 1 and 2, characterized in that a solution of calcium lactate or calcium citrate is taken as a solution of edible calcium salt. 4. The method according to any one of claims 1 and 2, characterized in that the rhizoids are cut manually or on circular knives. 5. The method according to any one of claims 1 and 2, characterized in that the thalli are washed with sea water in a cascade or swirl wash or by showering. 6. The method according to any one of claims 1 and 2, characterized in that in the case of spirorbis on the surface of the thalli, they are cleaned with brushes. 7. The method according to any one of claims 1 and 2, characterized in that the dry algae is washed in fresh water by showering in a bath, washing off sand and salt. 8. The method according to any one of claims 1 and 2, characterized in that for washing using natural drinking water with a hardness of up to 1 mol / m ³ or softened (corrected) drinking water with a pH of not more than 7.8 and hardness of up to 0, 2 mol / m ³ , washed algae is crushed in a meat grinder or pulper. 9. The method according to any one of claims 1 and 2, characterized in that for cooking the washed algae is crushed and filled with hot water in a cooking vessel with a ratio of supplied heating power and net volume equal to 0.20-0.25 kW / l, for example , in a reactor with a thermostatically controlled jacket and a stirrer, with a sleeveless space for heating with dry steam, or in an electric boiler with a stirrer. 10. The method according to any one of claims 1 and 2, characterized in that when neutralized, flavoring additives are added to the mass, and after reaching an acid value of pH 7.0-8.0, immediately before cooking, add steviazide in an amount up to 1 mg per 1% of the mass of algae. 11. The method according to any one of claims 1 and 2, characterized in that before converting to a form suitable for storage, the iodine content, which should be at least 15 mg / kg, of carbohydrate fractions, which should be: mannat, is controlled in the gel obtained. 133.4 ± 12.1 mg / g, alginic acid 147.8 ± 10.7 mg / g, and also check the hygienic characteristics in terms of the content of toxic elements: lead not more than 0.5 mg / kg, arsenic not more than 0, 5 mg / kg, cadmium not more than 1.0 mg / kg, mercury not more than 0.1 mg / kg, DDT not more than 2.0 mg / kg, radionuclides not more than 6 k / kg. 12. The method according to claim 11, characterized in that for converting into a form suitable for storage, the gel is Packed in hot form or pre-drying, and drying is carried out using a freeze dryer, or a vacuum dryer, or a spray dryer , or convection-drying plants, while the prepared pasteurized mass is poured onto the tubes or cuvettes with a layer of not more than 10 mm, which are placed on shelves in a drying unit, where the mass is dried to a final moisture content of not more than 10%.