CN109790510B - 在不存在诱导底物下丝状真菌细胞中的蛋白产生 - Google Patents

在不存在诱导底物下丝状真菌细胞中的蛋白产生 Download PDF

Info

Publication number
CN109790510B
CN109790510B CN201780059992.7A CN201780059992A CN109790510B CN 109790510 B CN109790510 B CN 109790510B CN 201780059992 A CN201780059992 A CN 201780059992A CN 109790510 B CN109790510 B CN 109790510B
Authority
CN
China
Prior art keywords
seq
ace3
protein
promoter
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201780059992.7A
Other languages
English (en)
Chinese (zh)
Other versions
CN109790510A (zh
Inventor
M·华德
Y·罗
F·O·本德苏
M·瓦尔科宁
M·萨罗海摩
N·阿罗
T·帕库拉
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Vtt Technical Res Centre Of Finland Ltd
Danisco US Inc
Original Assignee
Vtt Technical Res Centre Of Finland Ltd
Danisco US Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Vtt Technical Res Centre Of Finland Ltd, Danisco US Inc filed Critical Vtt Technical Res Centre Of Finland Ltd
Publication of CN109790510A publication Critical patent/CN109790510A/zh
Application granted granted Critical
Publication of CN109790510B publication Critical patent/CN109790510B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Botany (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CN201780059992.7A 2016-10-04 2017-10-03 在不存在诱导底物下丝状真菌细胞中的蛋白产生 Active CN109790510B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662403787P 2016-10-04 2016-10-04
US62/403,787 2016-10-04
PCT/US2017/054983 WO2018067599A1 (en) 2016-10-04 2017-10-03 Protein production in filamentous fungal cells in the absence of inducing substrates

Publications (2)

Publication Number Publication Date
CN109790510A CN109790510A (zh) 2019-05-21
CN109790510B true CN109790510B (zh) 2024-04-12

Family

ID=60245185

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201780059992.7A Active CN109790510B (zh) 2016-10-04 2017-10-03 在不存在诱导底物下丝状真菌细胞中的蛋白产生

Country Status (6)

Country Link
US (2) US10876103B2 (https=)
EP (1) EP3523415A1 (https=)
JP (2) JP7285780B2 (https=)
KR (1) KR102593668B1 (https=)
CN (1) CN109790510B (https=)
WO (1) WO2018067599A1 (https=)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110760537A (zh) * 2018-07-27 2020-02-07 中国农业科学院饲料研究所 用于快速筛选重组菌株的重组表达载体及应用
JP7609774B2 (ja) * 2018-11-28 2025-01-07 ノボザイムス アクティーゼルスカブ 修飾された糸状菌宿主細胞
WO2021007630A1 (pt) * 2019-07-16 2021-01-21 Centro Nacional De Pesquisa Em Energia E Materiais Linhagem de fungo trichoderma modificada para a produção de coquetel enzimático
FI4055177T3 (fi) * 2019-11-08 2024-10-31 Danisco Us Inc Parantuneen proteiinituottavuuden fenotyyppejä käsittäviä sienikantoja ja menetelmiä niitä varten
CN114729385B (zh) * 2019-11-18 2025-03-11 花王株式会社 突变丝状菌和使用其的蛋白质的制造方法
CN113106114B (zh) * 2020-01-13 2024-04-12 中国科学院分子植物科学卓越创新中心 调控里氏木霉蛋白表达效率的因子、调控方法及应用
EP4139332A1 (en) 2020-04-22 2023-03-01 Danisco US Inc. Compositions and methods for enhanced protein production in filamentous fungal cells
JP7827410B2 (ja) * 2021-04-09 2026-03-10 花王株式会社 改変糸状菌、及びそれを用いたタンパク質の製造方法
CN116813728B (zh) * 2023-07-07 2024-08-09 山东大学 一种真菌木质纤维素降解酶合成调控蛋白突变体及其应用
CN118389304A (zh) * 2023-12-28 2024-07-26 福州道福生物科技有限公司 一种生产索马甜ⅰ蛋白的重组里氏木霉菌株及其构建方法与应用
WO2025165968A1 (en) 2024-01-31 2025-08-07 Danisco Us Inc. Conditional regulation of protein function in filamentous fungal cells

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2005229437A1 (en) * 2004-04-02 2005-10-13 Dsm Ip Assets B.V. Filamentous fungal mutants with improved homologous recombination efficiency
CN101831452A (zh) * 2010-04-22 2010-09-15 中国农业科学院生物技术研究所 通过重组瑞氏木霉以诱导型方式高效表达和生产t4溶菌酶的方法
CN102099460A (zh) * 2008-04-08 2011-06-15 丹尼斯科美国公司 用于改变的蛋白质产生的具有失活蛋白酶基因的丝状真菌
WO2011151512A2 (en) * 2010-06-04 2011-12-08 Teknologian Tutkimuskeskus Vtt Method for protein production in filamentous fungi
CA2801799A1 (en) * 2010-06-03 2011-12-08 Danisco Us Inc. Filamentous fungal host strains and dna constructs, and methods of use thereof
CN102311951A (zh) * 2011-07-13 2012-01-11 深圳大学 里氏木霉组成型表达盒、表达载体、重组菌株及其应用
CN105980552A (zh) * 2013-07-10 2016-09-28 诺华股份有限公司 多种蛋白酶缺陷的丝状真菌细胞及其使用方法
CN106978360A (zh) * 2017-04-24 2017-07-25 上海交通大学 一株高产纤维素酶里氏木霉重组菌株及其应用
CN113106114A (zh) * 2020-01-13 2021-07-13 中国科学院分子植物科学卓越创新中心 调控里氏木霉蛋白表达效率的因子、调控方法及应用

Family Cites Families (54)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3650202T3 (de) 1985-08-29 2004-06-03 Genencor International, Inc., South San Francisco Expression von heterologen Polypeptiden in filamentösen Pilzen, Verfahren zu deren Herstellung und Vektoren zu deren Herstellung.
GB8610600D0 (en) 1986-04-30 1986-06-04 Novo Industri As Transformation of trichoderma
WO1992006221A1 (en) 1990-10-05 1992-04-16 Genencor International, Inc. Methods for treating cotton-containing fabrics with cellulase
WO1992006183A1 (en) 1990-10-05 1992-04-16 Genencor International, Inc. Methods for treating cotton-containing fabrics with cellulase
DK0553280T4 (da) 1990-10-05 2010-11-15 Genencor Int Fremgangsmåde til behandling af bomuldsholdige tekstiler med cellulase
WO1992010581A1 (en) 1990-12-10 1992-06-25 Genencor International, Inc. IMPROVED SACCHARIFICATION OF CELLULOSE BY CLONING AND AMPLIFICATION OF THE β-GLUCOSIDASE GENE OF TRICHODERMA REESEI
US6017870A (en) 1996-10-09 2000-01-25 Genencor International, Inc. Purified cellulase and method of producing
US6255115B1 (en) 1997-04-07 2001-07-03 Unilever Patent Holdings Bv Agrobacterium mediated transformation of moulds, in particular those belonging to the genus Aspergillus
US6268328B1 (en) 1998-12-18 2001-07-31 Genencor International, Inc. Variant EGIII-like cellulase compositions
EP1305432B1 (en) 2000-08-04 2010-09-15 Genencor International, Inc. Mutant trichoderma reesei egiii cellulases, dna encoding such egiii compositions and methods for obtaining same
US6982159B2 (en) 2001-09-21 2006-01-03 Genencor International, Inc. Trichoderma β-glucosidase
US7056721B2 (en) 2001-12-18 2006-06-06 Genencor International, Inc. EGVI endoglucanase and nucleic acids encoding the same
US7049125B2 (en) 2001-12-18 2006-05-23 Genencor International, Inc. EGVIII endoglucanase and nucleic acids encoding the same
US7045332B2 (en) 2001-12-18 2006-05-16 Genencor International, Inc. BGL4 β-glucosidase and nucleic acids encoding the same
US7045331B2 (en) 2001-12-18 2006-05-16 Genencor International, Inc. EGVII endoglucanase and nucleic acids encoding the same
US7005289B2 (en) 2001-12-18 2006-02-28 Genencor International, Inc. BGL5 β-glucosidase and nucleic acids encoding the same
WO2004016760A2 (en) 2002-08-16 2004-02-26 Genencor International, Inc. Novel variant hyprocrea jecorina cbh1 cellulases
CA2498213C (en) 2002-09-10 2013-07-16 Genencor International, Inc. Induction of gene expression using a high concentration sugar mixture
AU2003291395A1 (en) 2002-11-07 2004-06-03 Genencor International, Inc. Bgl6 beta-glucosidase and nucleic acids encoding the same
US7407788B2 (en) 2002-11-21 2008-08-05 Danisco A/S, Genencor Division BGL7 beta-glucosidase and nucleic acids encoding the same
EP2311942A1 (en) 2003-03-21 2011-04-20 Genecor International, Inc. Novel cbh1 homologs and variant cbh1 cellulases
CN101374948B (zh) 2003-04-01 2012-04-11 金克克国际有限公司 灰腐质霉cbh1.1变体
ATE524491T1 (de) 2003-05-29 2011-09-15 Genencor Int Neue trichoderma-gene
US8097445B2 (en) 2004-03-25 2012-01-17 Danisco Us Inc. Exo-endo cellulase fusion protein
EP2354222A1 (en) 2004-03-25 2011-08-10 Genencor International, Inc. Cellulase fusion protein and heterologous cellulase fusion construct encoding the same
US7413887B2 (en) 2004-05-27 2008-08-19 Genecor International, Inc. Trichoderma reesei glucoamylase and homologs thereof
JP5148288B2 (ja) 2004-12-30 2013-02-20 ジェネンコー・インターナショナル・インク 新規変異体ハイポクレアジェコリーナｃｂｈ２セルラーゼ
US20070231819A1 (en) * 2006-03-15 2007-10-04 Christopher Lawrence Targeted and non-targeted gene insertions using a linear minimal element construct
US8143046B2 (en) 2007-02-07 2012-03-27 Danisco Us Inc., Genencor Division Variant Buttiauxella sp. phytases having altered properties
EP2288698B1 (en) 2008-06-06 2016-12-21 Danisco US Inc. Compositions and methods comprising cellulase variants with reduced affinity to non-cellulosic materials
WO2010141779A1 (en) 2009-06-03 2010-12-09 Danisco Us Inc. Cellulase variants with improved expression, activity and/or stability, and use thereof
DK2480660T5 (da) 2009-09-23 2020-11-09 Danisco Us Inc Hidtil ukendte glycosylhydrolaseenzymer og anvendelser heraf
IN2012DN03825A (https=) 2009-11-20 2015-08-28 Danisco Us Inc
CN102884086B (zh) 2010-06-01 2015-11-25 加州理工学院 稳定的功能嵌合纤维二糖水解酶i类酶
US20120107872A1 (en) * 2010-10-20 2012-05-03 Andrei Miasnikov Thermostable trichoderma cellulase
CN103492561A (zh) 2011-03-17 2014-01-01 丹尼斯科美国公司 纤维素酶组合物和使用相同组合物用于改善木质纤维素生物质转化成可发酵糖的方法
JP6290626B2 (ja) 2011-03-17 2018-03-14 ダニスコ・ユーエス・インク 糖化プロセスにおける粘度を低下させる方法
RU2013146240A (ru) 2011-03-17 2015-04-27 ДАНИСКО ЮЭс ИНК. Ферменты гликозил-гидролазы и их применение для гидролиза биомассы
WO2013096056A1 (en) 2011-12-21 2013-06-27 Danisco Us Inc. Improving fungal gene expression using insulator dna sequences
DK2800809T3 (en) 2012-01-05 2018-05-22 Glykos Finland Oy PROTEASE-DEFECTED FILAMENTARY FUNGAL CELLS AND PROCEDURES FOR USE THEREOF
US10030221B2 (en) 2012-09-20 2018-07-24 Danisco Us Inc. Microtiter plates for controlled release of culture components to cell cultures
US20150252344A1 (en) 2012-10-31 2015-09-10 Danisco Us Inc. Beta-glucosidase from neurospora crassa
CA2888753A1 (en) 2012-10-31 2014-05-08 Danisco Us Inc. Beta-glucosidase from magnaporthe grisea
EP2914719A1 (en) 2012-10-31 2015-09-09 Danisco US Inc. Compositions and methods of use
EP2931887A1 (en) 2012-12-12 2015-10-21 Danisco US Inc. Variants of cellobiohydrolases
CA2893053A1 (en) 2012-12-12 2014-06-19 Danisco Us Inc. Variants of cellobiohydrolases
BR112015013759A2 (pt) 2012-12-12 2017-11-14 Danisco Us Inc variante isolada de uma enzima celobio-hidrolase (cbh) parental, polinucleotídeo isolado, vetor, célula hospedeira, composição detergente, aditivo alimentar, método para hidrolisar um substrato celulósico, sobrenadante de cultura celular, métodos de produção de um polipeptídeo de variante de cbh e sobrenadante de cultura celular
EP2931886A1 (en) 2012-12-12 2015-10-21 Danisco US Inc. Variants of cellobiohydrolases
US20150315560A1 (en) 2012-12-12 2015-11-05 Danisco Us Inc. Variants of cellobiohydrolases
BR112016012627A2 (pt) 2013-12-04 2017-09-26 Danisco Us Inc composições compreendendo um polipeptídeo de beta-glicosidase e métodos de uso
AR102445A1 (es) 2014-10-27 2017-03-01 Danisco Us Inc COMPOSICIONES Y MÉTODOS RELACIONADOS CON b-GLUCOSIDASA
CA2971187C (en) 2014-12-16 2023-10-24 Danisco Us Inc. Fungal genome modification systems and methods of use
WO2017177289A1 (en) 2016-04-15 2017-10-19 Vtt International Oy Process for conversion of biomass into fermentable sugars with integrated enzyme
EP4139332A1 (en) * 2020-04-22 2023-03-01 Danisco US Inc. Compositions and methods for enhanced protein production in filamentous fungal cells

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2005229437A1 (en) * 2004-04-02 2005-10-13 Dsm Ip Assets B.V. Filamentous fungal mutants with improved homologous recombination efficiency
CN102099460A (zh) * 2008-04-08 2011-06-15 丹尼斯科美国公司 用于改变的蛋白质产生的具有失活蛋白酶基因的丝状真菌
CN101831452A (zh) * 2010-04-22 2010-09-15 中国农业科学院生物技术研究所 通过重组瑞氏木霉以诱导型方式高效表达和生产t4溶菌酶的方法
CA2801799A1 (en) * 2010-06-03 2011-12-08 Danisco Us Inc. Filamentous fungal host strains and dna constructs, and methods of use thereof
CN102939382A (zh) * 2010-06-03 2013-02-20 丹尼斯科美国公司 丝状真菌宿主菌株和dna构建体以及它们的使用方法
WO2011151512A2 (en) * 2010-06-04 2011-12-08 Teknologian Tutkimuskeskus Vtt Method for protein production in filamentous fungi
CN102311951A (zh) * 2011-07-13 2012-01-11 深圳大学 里氏木霉组成型表达盒、表达载体、重组菌株及其应用
CN105980552A (zh) * 2013-07-10 2016-09-28 诺华股份有限公司 多种蛋白酶缺陷的丝状真菌细胞及其使用方法
CN106978360A (zh) * 2017-04-24 2017-07-25 上海交通大学 一株高产纤维素酶里氏木霉重组菌株及其应用
CN113106114A (zh) * 2020-01-13 2021-07-13 中国科学院分子植物科学卓越创新中心 调控里氏木霉蛋白表达效率的因子、调控方法及应用

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
Comparative Genomics Analysis of Trichoderma reesei Strains;Hideaki Koike 等;《INDUSTRIAL BIOTECHNOLOGY》;20131231;全文 *
Engineering of Trichoderma reesei for enhanced degradation of lignocellulosic biomass by truncation of the cellulase activator ACE3;Chen Y 等;《Biotechnol Biofuels》;20200401;第13卷(第62期);全文 *
hypothetical protein M419DRAFT_98455 [Trichoderma reesei RUT C-30]ETS02557.1;Kuo,A 等;《Genbank Database》;20150323;全文 *
Modification of transcriptional factor ACE3 enhances protein production in Trichoderma reesei in the absence of cellulase gene inducer;Luo Y 等;《Biotechnol Biofuels》;20200806;第13卷(第137期);全文 *
Promoting cellulase and hemicellulase production from Trichoderma orientalis EU7-22 by overexpression of transcription factors Xyr1 and Ace3;Xue Y 等;《Bioresour Technol》;20191101;全文 *
The effects of disruption of phosphoglucose isomerase gene on carbon utilisation and cellulase production in Trichoderma reesei Rut-C30;M Carmen Limón 等;《Microb Cell Fact》;20110524;第10卷(第40期);全文 *
Trichoderma reesei RUT-C30 – thirty years of strain improvement;Robyn Peterson;《microbiology》;20111013;第158卷(第1期);全文 *
转录激活因子Xyr1在里氏木霉纤维素酶基因诱导表达中的作用机制研究;郑芳林;中国博士学位论文库全文库;全文 *
里氏木霉代谢工程改造及纤维素合成调控;赵心清;中国会议;全文 *
里氏木霉双基因同步定点整合体系的构建及提高β-甘露聚糖酶表达的策略研究;吴鸿清;《中国优秀硕士学位论文全文数据库（电子期刊）基础科学辑》;20150515;全文 *

Also Published As

Publication number Publication date
US20190309276A1 (en) 2019-10-10
US10876103B2 (en) 2020-12-29
KR20190057088A (ko) 2019-05-27
CN109790510A (zh) 2019-05-21
US20220145278A1 (en) 2022-05-12
JP7605902B2 (ja) 2024-12-24
JP2019528797A (ja) 2019-10-17
WO2018067599A1 (en) 2018-04-12
KR102593668B1 (ko) 2023-10-24
EP3523415A1 (en) 2019-08-14
JP7285780B2 (ja) 2023-06-02
JP2023109919A (ja) 2023-08-08

Similar Documents

Publication Publication Date Title
CN109790510B (zh) 在不存在诱导底物下丝状真菌细胞中的蛋白产生
WO2017093450A1 (en) Method of producing proteins in filamentous fungi with decreased clr2 activity
WO2017093451A1 (en) Method of producing proteins in filamentous fungi with decreased clri activity
JP7648614B2 (ja) タンパク質生産性が増強された表現型を含む糸状菌株及びその方法
JP7799621B2 (ja) 糸状菌細胞におけるタンパク質産生の増強のための組成物及び方法
US20250320512A1 (en) Compositions and methods for enhanced protein production in fungal cells
US20240376511A1 (en) Compositions and methods for enhanced protein production in fungal cells
JP2025537244A (ja) タンパク質生産性が増強された表現型を含む糸状菌株及びその方法
WO2025165968A1 (en) Conditional regulation of protein function in filamentous fungal cells
JP2026500088A (ja) 一貫したタンパク質を生成するための組換え真菌株及びその方法

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant