CN109750069B - 生产l-赖氨酸的重组菌、其构建方法以及l-赖氨酸的生产方法 - Google Patents

生产l-赖氨酸的重组菌、其构建方法以及l-赖氨酸的生产方法

Info

Publication number
CN109750069B
CN109750069B CN201711058221.4A CN201711058221A CN109750069B CN 109750069 B CN109750069 B CN 109750069B CN 201711058221 A CN201711058221 A CN 201711058221A CN 109750069 B CN109750069 B CN 109750069B
Authority
CN
China
Prior art keywords
expression
activity
gene
recombinant bacterium
lysine
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201711058221.4A
Other languages
English (en)
Chinese (zh)
Other versions
CN109750069A (zh
Inventor
温廷益
张琛
商秀玲
柴欣
张芸
刘树文
王国强
李忠财
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institute of Microbiology of CAS
Ningxia Eppen Biotech Co Ltd
Original Assignee
Beijing Zhongke Yipin Biotechnology Co ltd
Institute of Microbiology of CAS
Ningxia Eppen Biotech Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to CN201711058221.4A priority Critical patent/CN109750069B/zh
Application filed by Beijing Zhongke Yipin Biotechnology Co ltd, Institute of Microbiology of CAS, Ningxia Eppen Biotech Co Ltd filed Critical Beijing Zhongke Yipin Biotechnology Co ltd
Priority to CA3081113A priority patent/CA3081113A1/en
Priority to JP2020524437A priority patent/JP6961819B2/ja
Priority to PCT/CN2018/087894 priority patent/WO2019085445A1/zh
Priority to EP18874429.6A priority patent/EP3690027A4/en
Priority to US16/760,662 priority patent/US20200347419A1/en
Publication of CN109750069A publication Critical patent/CN109750069A/zh
Priority to PH12020550529A priority patent/PH12020550529A1/en
Application granted granted Critical
Publication of CN109750069B publication Critical patent/CN109750069B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/69—Increasing the copy number of the vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C12N9/82—Asparaginase (3.5.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/165—Yeast isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C12N1/185—Saccharomyces isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/13—Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/15—Corynebacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/72—Candida
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/78—Hansenula
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/84—Pichia
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/85—Saccharomyces
    • C12R2001/865—Saccharomyces cerevisiae

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201711058221.4A 2017-11-01 2017-11-01 生产l-赖氨酸的重组菌、其构建方法以及l-赖氨酸的生产方法 Active CN109750069B (zh)

Priority Applications (7)

Application Number Priority Date Filing Date Title
CN201711058221.4A CN109750069B (zh) 2017-11-01 2017-11-01 生产l-赖氨酸的重组菌、其构建方法以及l-赖氨酸的生产方法
JP2020524437A JP6961819B2 (ja) 2017-11-01 2018-05-22 Ｌ−リジンを生産する組換え菌、その構築方法およびｌ−リジンの生産方法
PCT/CN2018/087894 WO2019085445A1 (zh) 2017-11-01 2018-05-22 生产l-赖氨酸的重组菌、其构建方法以及l-赖氨酸的生产方法
EP18874429.6A EP3690027A4 (en) 2017-11-01 2018-05-22 RECOMBINANT BACTERIA FOR L-LYSINE PRODUCTION, ITS CONSTRUCTION PROCESS AND L-LYSINE PRODUCTION PROCESS
CA3081113A CA3081113A1 (en) 2017-11-01 2018-05-22 Recombinant bacterium capable of producing l-lysine, construction method thereof and production method of l-lysine
US16/760,662 US20200347419A1 (en) 2017-11-01 2018-05-22 Recombinant bacterium capable of producing l-lysine, construction method thereof and production method of l-lysine
PH12020550529A PH12020550529A1 (en) 2017-11-01 2020-04-30 Recombinant bacterium capable of producing l-lysine, construction method thereof and production method of l-lysine

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201711058221.4A CN109750069B (zh) 2017-11-01 2017-11-01 生产l-赖氨酸的重组菌、其构建方法以及l-赖氨酸的生产方法

Publications (2)

Publication Number Publication Date
CN109750069A CN109750069A (zh) 2019-05-14
CN109750069B true CN109750069B (zh) 2025-08-01

Family

ID=66331288

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201711058221.4A Active CN109750069B (zh) 2017-11-01 2017-11-01 生产l-赖氨酸的重组菌、其构建方法以及l-赖氨酸的生产方法

Country Status (7)

Country Link
US (1) US20200347419A1 (https=)
EP (1) EP3690027A4 (https=)
JP (1) JP6961819B2 (https=)
CN (1) CN109750069B (https=)
CA (1) CA3081113A1 (https=)
PH (1) PH12020550529A1 (https=)
WO (1) WO2019085445A1 (https=)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110256535B (zh) * 2019-06-14 2021-01-01 中国石油大学(华东) L-天门冬酰胺酶XiDL及其编码基因和应用
AU2020307380A1 (en) * 2019-06-24 2022-01-27 Danisco Us Inc Disruption of Cdc42 effectors in yeast for increased alcohol and lysine production
CN110592109B (zh) * 2019-08-28 2020-10-09 黑龙江伊品生物科技有限公司 一种spoT基因改造的重组菌株及其构建方法与应用
CN112877269B (zh) 2020-01-15 2021-12-24 中国科学院天津工业生物技术研究所 生产赖氨酸的微生物以及赖氨酸的生产方法
CN111607608B (zh) * 2020-04-20 2023-04-14 天津科技大学 基因工程高产菌株淀粉酶产色链霉菌及提高ε-聚赖氨酸产量的方法和应用
CN111850010B (zh) * 2020-06-08 2021-04-09 黑龙江伊品生物科技有限公司 一种dapB基因改造的重组菌株及其构建方法与应用
CN111979165B (zh) * 2020-08-07 2021-05-07 黑龙江伊品生物科技有限公司 一种产l-赖氨酸的重组菌株及其构建方法与应用
CN113755492B (zh) * 2020-07-20 2023-05-30 中国科学院天津工业生物技术研究所 丙酮酸羧化酶基因启动子的突变体及其应用
CN112301068B (zh) * 2020-10-29 2022-10-04 齐齐哈尔龙江阜丰生物科技有限公司 一种葡萄糖母液水解综合利用工艺
BR112023018219A2 (pt) * 2021-03-09 2023-11-28 Daesang Corp Variante de corynebacterium glutamicum que tem capacidade de produção de l-lisina aprimorada e método para produzir l-lisina com o uso da mesma
CN112695036B (zh) * 2021-03-23 2021-07-06 中国科学院天津工业生物技术研究所 一种天冬氨酸激酶基因表达调控序列及其应用
BR112023021475A2 (pt) * 2021-04-16 2023-12-19 Novartis Ag Conjugados anticorpo-fármaco e métodos para produzir os mesmos
CA3217149A1 (en) * 2021-04-29 2022-11-03 Young Ju Lee Corynebacterium glutamicum variant having improved l-lysine production ability, and method for producing l-lysine using same
BR112023022652A2 (pt) * 2021-04-30 2024-01-16 Cj Cheiljedang Corp Promotor, cepa mutante de corynebacterium glutamicum e método de produção de l-lisina
CN113278571A (zh) * 2021-05-27 2021-08-20 齐鲁工业大学 一种棒状杆菌工程菌构建方法及应用
CN115449519B (zh) * 2021-06-08 2023-04-07 中国科学院天津工业生物技术研究所 基于dapB基因的具有启动子活性的多核苷酸及其用途
CN116179586A (zh) * 2021-11-26 2023-05-30 上海凯赛生物技术股份有限公司 用于发酵生产l-赖氨酸的表达盒、菌株及其应用
CN114908027B (zh) * 2022-01-20 2023-12-26 天津科技大学 泛酸生产相关菌株及其构建方法与应用
CN116555131A (zh) * 2022-01-27 2023-08-08 廊坊梅花生物技术开发有限公司 一种重组微生物及其构建方法和应用
CN116004495B (zh) * 2022-10-12 2025-12-16 浙江工业大学 生产β-丙氨酸的重组基因工程菌及其应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102753692A (zh) * 2010-06-15 2012-10-24 白光产业株式会社 使用微生物生产天冬氨酸族氨基酸的方法
CN105734004A (zh) * 2016-03-02 2016-07-06 廊坊梅花生物技术开发有限公司 重组菌株及其制备方法、应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MXPA01013123A (es) * 1999-06-25 2002-06-21 Basf Ag Genes de corynebacterium glutamicum que codifican proteinas de via metabolica.
JP4623825B2 (ja) * 1999-12-16 2011-02-02 協和発酵バイオ株式会社 新規ポリヌクレオチド
DE102004035065A1 (de) * 2004-07-20 2006-02-16 Basf Ag P-ET-TS-Expressionseinheiten
EP2440669A4 (en) * 2009-06-10 2013-08-28 Genomatica Inc MICROOGANISMS AND PROCEDURES FOR THE CARBON EFFECTIVE BIOSYNTHESIS MEK AND 2-BUTANOL
CN103003297B (zh) * 2010-07-14 2016-06-01 雀巢产品技术援助有限公司 担子菌的天冬酰胺酶

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102753692A (zh) * 2010-06-15 2012-10-24 白光产业株式会社 使用微生物生产天冬氨酸族氨基酸的方法
CN105734004A (zh) * 2016-03-02 2016-07-06 廊坊梅花生物技术开发有限公司 重组菌株及其制备方法、应用

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Characterization and partial purification of L-asparaginase from Corynebacterium glutamicum;Mesas J. M. et al.;《Journal of General Microbiology》;19901231;515-519 *
Mesas J. M. et al..Characterization and partial purification of L-asparaginase from Corynebacterium glutamicum.《Journal of General Microbiology》.1990,515-519. *

Also Published As

Publication number Publication date
CA3081113A1 (en) 2019-05-09
JP2021500914A (ja) 2021-01-14
PH12020550529A1 (en) 2021-04-26
JP6961819B2 (ja) 2021-11-05
CN109750069A (zh) 2019-05-14
EP3690027A1 (en) 2020-08-05
US20200347419A1 (en) 2020-11-05
WO2019085445A1 (zh) 2019-05-09
EP3690027A4 (en) 2020-11-11

Similar Documents

Publication Publication Date Title
CN109750069B (zh) 生产l-赖氨酸的重组菌、其构建方法以及l-赖氨酸的生产方法
JP2021500914A5 (https=)
DK2236610T3 (en) Promoter and improved method for producing L-lysine using the same
JP2018518977A (ja) Ｌ−リジンを生産する微生物及びそれを用いたｌ−リジン生産方法
CN110117568B (zh) 生产l-组氨酸的重组菌、其构建方法以及l-组氨酸的生产方法
JP2023550754A (ja) シュワネラ・オネイデンシス由来蛋白質を発現する微生物、およびこれを利用したｌ－アミノ酸の生産方法
JP7350994B2 (ja) 新規なプロモーター及びそれを用いた標的物質生産方法
EP4538375A1 (en) Ep6 promoter, related biomaterial, and use thereof
CN111411092B (zh) 高产l-赖氨酸的谷氨酸棒状杆菌及其应用
CN115029289B (zh) 高产l-苏氨酸的基因工程菌及其构建方法与应用
CN100334200C (zh) 一株可高产谷氨酰胺的谷氨酸棒杆菌
CN101611136B (zh) 编码钼辅助因子生物合成酶A的moaA基因的表达增加的谷氨酸棒杆菌及用该棒杆菌生产L-赖氨酸的方法
JP7447294B2 (ja) シュワネラ・アトランティカ由来蛋白質を発現する微生物、およびこれを利用したｌ－アミノ酸生産方法
CN118207232A (zh) 构建l-缬氨酸生产菌株的方法、l-缬氨酸生产菌株及其应用
CN116286807A (zh) 突变的dapA启动子及其应用
CN117778226A (zh) L-赖氨酸生产能力得到提高的谷氨酸棒状杆菌突变株及利用其的l-赖氨酸的生产方法
CN115261246A (zh) L-赖氨酸生产能力得到提高的谷氨酸棒状杆菌突变株及利用其的l-赖氨酸的生产方法
CN118126995B (zh) 一种ppc突变体及其在L-缬氨酸发酵生产中的应用
CN116064349A (zh) L-赖氨酸生产能力得到提高的谷氨酸棒状杆菌突变株及利用其的l-赖氨酸的生产方法
CN116286809A (zh) 一种突变的gluB启动子及其应用
CN116555134A (zh) 产苏氨酸菌株的构建方法
CN117511834A (zh) L-赖氨酸生产能力得到提高的谷氨酸棒状杆菌突变株及利用其的l-赖氨酸的生产方法
CN119506324A (zh) 一种修饰的棒杆菌属微生物及其在赖氨酸发酵生产中的应用
CN120041475A (zh) 一种赖氨酸人工操纵子及其应用
CN117987444A (zh) 表达盒、含其的基因工程菌及其在l-赖氨酸生产中的应用

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
TA01 Transfer of patent application right

Effective date of registration: 20241108

Address after: 750100 Yang Yang Industrial Park, Yongning County, the Ningxia Hui Autonomous Region, Yinchuan

Applicant after: NINGXIA EPPEN BIOTECH Co.,Ltd.

Country or region after: China

Applicant after: INSTITUTE OF MICROBIOLOGY, CHINESE ACADEMY OF SCIENCES

Address before: Room B-319, Zhongguancun Biomedical Park, No. 5 Shangdi Kaituo Road, Haidian District, Beijing 100085

Applicant before: BEIJING ZHONGKE YIPIN BIOTECHNOLOGY Co.,Ltd.

Country or region before: China

Applicant before: INSTITUTE OF MICROBIOLOGY, CHINESE ACADEMY OF SCIENCES

TA01 Transfer of patent application right
GR01 Patent grant
GR01 Patent grant