BR112023022652A2 - Promotor, cepa mutante de corynebacterium glutamicum e método de produção de l-lisina - Google Patents

Promotor, cepa mutante de corynebacterium glutamicum e método de produção de l-lisina

Info

Publication number
BR112023022652A2
BR112023022652A2 BR112023022652A BR112023022652A BR112023022652A2 BR 112023022652 A2 BR112023022652 A2 BR 112023022652A2 BR 112023022652 A BR112023022652 A BR 112023022652A BR 112023022652 A BR112023022652 A BR 112023022652A BR 112023022652 A2 BR112023022652 A2 BR 112023022652A2
Authority
BR
Brazil
Prior art keywords
corynebacterium glutamicum
promoter
production method
mutant strain
lysine production
Prior art date
Application number
BR112023022652A
Other languages
English (en)
Inventor
Ki Kim Bong
Eun Kim Ha
Hyun Park Joon
Jin CHOI Min
Hyun Park Seok
Original Assignee
Cj Cheiljedang Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from KR1020210066965A external-priority patent/KR20220149376A/ko
Application filed by Cj Cheiljedang Corp filed Critical Cj Cheiljedang Corp
Publication of BR112023022652A2 publication Critical patent/BR112023022652A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y604/00—Ligases forming carbon-carbon bonds (6.4)
    • C12Y604/01—Ligases forming carbon-carbon bonds (6.4.1)
    • C12Y604/01001—Pyruvate carboxylase (6.4.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/15—Corynebacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/265—Micrococcus
    • C12R2001/28—Micrococcus glutamicus ; Corynebacterium glutamicum

Abstract

a presente invenção refere-se a uma variante de corynebacterium glutamicum que tem capacidade de produção de l-lisina melhorada e um método para produzir l-lisina com o uso da mesma. a variante aumenta ou intensifica a expressão de um gene que codifica a piruvato carboxilase, e então pode ter um rendimento de produção superior àquele de uma cepa parental.
BR112023022652A 2021-04-30 2021-05-26 Promotor, cepa mutante de corynebacterium glutamicum e método de produção de l-lisina BR112023022652A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR20210056581 2021-04-30
KR1020210066965A KR20220149376A (ko) 2021-04-30 2021-05-25 L-라이신 생산능이 향상된 코리네박테리움 글루타미쿰 변이주 및 이를 이용한 l-라이신의 생산 방법
PCT/KR2021/006566 WO2022231055A1 (ko) 2021-04-30 2021-05-26 L-라이신 생산능이 향상된 코리네박테리움 글루타미쿰 변이주 및 이를 이용한 l-라이신의 생산 방법

Publications (1)

Publication Number Publication Date
BR112023022652A2 true BR112023022652A2 (pt) 2024-01-16

Family

ID=76971636

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112023022652A BR112023022652A2 (pt) 2021-04-30 2021-05-26 Promotor, cepa mutante de corynebacterium glutamicum e método de produção de l-lisina

Country Status (8)

Country Link
EP (2) EP4083197A1 (pt)
JP (1) JP2024515391A (pt)
KR (1) KR20240000418A (pt)
CN (2) CN115261247B (pt)
AU (1) AU2021442655A1 (pt)
BR (1) BR112023022652A2 (pt)
CA (1) CA3217309A1 (pt)
WO (1) WO2022231055A1 (pt)

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19831609B4 (de) * 1997-10-04 2009-11-12 Evonik Degussa Gmbh Verfahren zur Herstellung von Aminosäuren der Aspartat- und/oder Glutamatfamilie und im Verfahren einsetzbare Mittel
JP2000201692A (ja) * 1999-01-13 2000-07-25 Ajinomoto Co Inc 発酵法によるｌ―グルタミン酸の製造法
EP3170889A1 (en) * 2006-09-15 2017-05-24 CJ Cheiljedang Corporation A corynebacteria having enhanced l-lysine productivity and a method of producing l-lysine using the same
KR100838038B1 (ko) 2006-12-29 2008-06-12 씨제이제일제당 (주) Ｌ-라이신 생산능이 향상된 코리네박테리움 속 미생물 및그를 이용한 ｌ-라이신 생산 방법
EP3404101A3 (en) * 2011-12-21 2018-12-26 Cj Cheiljedang Corporation Method for producing l-lysine using microorganisms having ability to produce l-lysine
EP2762571A1 (de) * 2013-01-30 2014-08-06 Evonik Industries AG Mikroorganismus und Verfahren zur fermentativen Herstellung von Aminosäuren
KR101621243B1 (ko) * 2014-08-28 2016-05-16 씨제이제일제당 주식회사 L-라이신 생산능을 갖는 코리네박테리움 미생물 및 이를 이용한 l-라이신 생산 방법
US20200239897A1 (en) 2017-06-07 2020-07-30 Zymergen Inc. Promoters from corynebacterium glutamicum and uses thereof in regulating ancillary gene expression
CN109750069A (zh) * 2017-11-01 2019-05-14 北京中科伊品生物科技有限公司 生产l-赖氨酸的重组菌、其构建方法以及l-赖氨酸的生产方法
CN110951662B (zh) * 2019-12-26 2024-03-12 新疆梅花氨基酸有限责任公司 一种高产赖氨酸的棒状细菌及其构建方法与应用
KR102139806B1 (ko) 2020-02-13 2020-07-30 씨제이제일제당 (주) 변이형 LysE를 포함하는 미생물, 및 이를 이용한 L-아미노산 생산 방법
CN113278571A (zh) * 2021-05-27 2021-08-20 齐鲁工业大学 一种棒状杆菌工程菌构建方法及应用

Also Published As

Publication number Publication date
JP2024515391A (ja) 2024-04-09
EP4083197A1 (en) 2022-11-02
KR20240000418A (ko) 2024-01-02
AU2021442655A1 (en) 2023-11-16
EP4353827A2 (en) 2024-04-17
WO2022231055A1 (ko) 2022-11-03
CN115261247A (zh) 2022-11-01
CN115261247B (zh) 2024-04-02
CN117551593A (zh) 2024-02-13
CA3217309A1 (en) 2022-11-03

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