CN109673584A - A method of hatching rate is extended shelf life and improved suitable for northern birds hatching egg - Google Patents
A method of hatching rate is extended shelf life and improved suitable for northern birds hatching egg Download PDFInfo
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- CN109673584A CN109673584A CN201910162636.9A CN201910162636A CN109673584A CN 109673584 A CN109673584 A CN 109673584A CN 201910162636 A CN201910162636 A CN 201910162636A CN 109673584 A CN109673584 A CN 109673584A
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- 230000012447 hatching Effects 0.000 title claims abstract description 211
- 238000000034 method Methods 0.000 title claims abstract description 36
- 238000003860 storage Methods 0.000 claims abstract description 19
- 238000003958 fumigation Methods 0.000 claims abstract description 7
- 235000013601 eggs Nutrition 0.000 claims description 169
- 238000004659 sterilization and disinfection Methods 0.000 claims description 24
- 241000271566 Aves Species 0.000 claims description 20
- 238000005507 spraying Methods 0.000 claims description 4
- 241000272525 Anas platyrhynchos Species 0.000 claims description 3
- 238000010438 heat treatment Methods 0.000 claims description 3
- 210000001161 mammalian embryo Anatomy 0.000 abstract description 20
- 102000002322 Egg Proteins Human genes 0.000 abstract description 13
- 108010000912 Egg Proteins Proteins 0.000 abstract description 13
- 210000003278 egg shell Anatomy 0.000 abstract description 13
- 241000894006 Bacteria Species 0.000 abstract description 12
- 238000009395 breeding Methods 0.000 abstract description 12
- 230000001488 breeding effect Effects 0.000 abstract description 12
- 230000000694 effects Effects 0.000 abstract description 6
- 230000037308 hair color Effects 0.000 abstract description 4
- 230000013020 embryo development Effects 0.000 abstract description 3
- 230000006641 stabilisation Effects 0.000 abstract description 3
- 238000011105 stabilization Methods 0.000 abstract description 3
- 230000001954 sterilising effect Effects 0.000 abstract description 3
- 102000004190 Enzymes Human genes 0.000 abstract description 2
- 108090000790 Enzymes Proteins 0.000 abstract description 2
- 238000005138 cryopreservation Methods 0.000 abstract description 2
- 230000002045 lasting effect Effects 0.000 abstract description 2
- 244000005700 microbiome Species 0.000 abstract description 2
- 230000003902 lesion Effects 0.000 abstract 1
- 238000012360 testing method Methods 0.000 description 27
- 238000004321 preservation Methods 0.000 description 12
- 230000008859 change Effects 0.000 description 4
- 230000004888 barrier function Effects 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 2
- 244000052616 bacterial pathogen Species 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 230000008011 embryonic death Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 244000144977 poultry Species 0.000 description 2
- 238000000528 statistical test Methods 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 230000035899 viability Effects 0.000 description 2
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- 102000009027 Albumins Human genes 0.000 description 1
- 108010088751 Albumins Proteins 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000032823 cell division Effects 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000000249 desinfective effect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000032696 parturition Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 238000005057 refrigeration Methods 0.000 description 1
- 210000005000 reproductive tract Anatomy 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000035900 sweating Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The present invention relates to a kind of methods for extending shelf life and improving hatching rate suitable for northern birds hatching egg, the specific method is as follows: sterilizing during hatching egg is sterilized and saved twice before saving to hatching egg library, harmful bacteria and the microorganism on hatching egg surface can effectively be killed, hatching egg lesion is reduced, while reducing harm of the harmful bacteria in environment to hatching egg;It using 9 DEG C~12 DEG C of storage temperature, can both guarantee the minimum temperature of embryonic development, hatching egg is protected to lose hatchability from enduring cold, and the activity and the breeding of bacterium of enzyme can be inhibited;The relative humidity for using 75%~80% simultaneously, can effectively ensure that in the case where cryo-conservation, and the first young hair color after hatching is normal, and eggshell is wet normal;The lasting pre-temperature 1 hour pre-temperature mode every 5 days 1 time is taken, can effectively enhance the vitality of embryo, be conducive to fumigation, while be conducive to the stabilization of incubator temperature.
Description
Technical field
The present invention relates to birds hatching technique field, specifically a kind of north birds hatching egg that is suitable for can extend shelf life
With the method for improving hatching rate.
Background technique
Hatching of breeding eggs effect directly affects the productivity effect of farm, is an important finger for measuring farm's Growth Results
Mark.Wherein, hatching egg preservation is an important factor for influencing hatching of breeding eggs rate.Currently, majority poultry cultivation factory is using conventional both at home and abroad
Method save hatching egg, enter to incubate within 2 days~3 days usually after laying eggs, hatching egg chickling-surviving rate is preferable.But due to some objective originals
Cause causes hatching egg that cannot enter to incubate on time, thus the holding time of hatching egg is forced to extend.
With the extension of hatching egg holding time, hatching egg internal moisture is lost, so as to cause embryo's aging, the change of nutriment
Change and the breeding of residual bacterial, endanger embryonic development, reduce hatching of breeding eggs rate.Presently, there are raising hatching of breeding eggs rate preservation
Method, operating process is complicated, with high costs, is unfavorable for the use of enterprise.So invent it is a kind of easy to operate, using safe
The store method for improving hatching of breeding eggs rate can effectively improve the hatching rate of preservation ten days or more hatching eggs, improve the warp of enterprise
Ji benefit, to promote the development of poultry cultivation industry.
Summary of the invention
In view of the deficiencies in the prior art, being suitable for northern birds hatching egg the purpose of the present invention is to provide one kind can
The method for extending shelf life and improving hatching rate, includes the following steps:
S1 hatching egg is collected;
S2 hatching egg carries out the 1st hatching egg disinfection immediately after collecting;
Hatching egg after S3 disinfection enters selection room and selects qualified hatching egg;
S4 carries out the 2nd disinfection to qualified hatching egg;
Hatching egg after S5 disinfection is sent into the storage of hatching egg library;During hatching egg library stores, temperature setting is 9 DEG C~12 DEG C;Relatively
Humidity is set as 75%~80%;
The hatching of S6 incubator: the storage of hatching egg library ten days or more hatching eggs, hatching egg must carry out every 5 days 1 time pre-temperature before entering to incubate,
To improve the hatching rate of hatching egg.
On the basis of above scheme, the hatching egg is same duck group, same batch, same age in days, when the preservation of hatching egg
Between be 11 days~15 days.
On the basis of above scheme, hatching egg described in the step S6 carries out the 3rd disinfection in incubator before entering to incubate.
On the basis of above scheme, hatching egg library described in step S5 was every progress fumigation in 7 days, every 2 days to hatching egg
Library ground carries out spraying disinfection.
Hatching egg library is cleared away using bacterium on the basis of above scheme, described in step S5 carries out disinfection.
On the basis of above scheme, the every 5 days 1 time pre-temperature method of hatching egg described in step S6, hatching egg pre-temperature temperature is 25
DEG C~35 DEG C.
On the basis of above scheme, the every 5 days 1 time pre-temperature method of hatching egg described in step S6, when each pre-temperature of hatching egg
Between be 1 hour.
The present invention has the following advantages compared with the prior art and effect: the present invention provides one kind can suitable for northern birds hatching egg
The method for extending shelf life and improving hatching rate can effectively improve the hatching rate of preservation ten days or more hatching eggs;Hatching egg before saving
Hatching egg library is sterilized in being sterilized and saved twice, effectively kills harmful bacteria and the microorganism on hatching egg surface, reduces hatching egg disease
Become, while reducing harm of the harmful bacteria in environment to hatching egg;Using 9 DEG C~12 DEG C of storage temperature, it can both guarantee embryo
The minimum temperature of survival protects hatching egg to lose hatchability from enduring cold, and can inhibit the activity and the breeding of bacterium of enzyme;
The relative humidity for using 75%~80% simultaneously, can effectively ensure that in the case where cryo-conservation, and the first young hair color after hatching is just
Often, eggshell is wet normal;The lasting pre-temperature 1 hour pre-temperature mode every 5 days 1 time is taken, the life of embryo can be effectively enhanced
Power is conducive to fumigation, while being conducive to the stabilization of incubator temperature.
Specific embodiment
A method of hatching rate can be extended shelf life and improve suitable for northern birds hatching egg, the specific method is as follows:
Hatching egg carries out the 1st hatching egg disinfection immediately after collecting, the hatching egg after disinfection enters selection room and selects qualified hatching egg simultaneously
2nd disinfection is carried out to qualified hatching egg, the hatching egg after disinfection is sent into the storage of hatching egg library;
During hatching egg saves, storage temperature is set as 9 DEG C~12 DEG C;
During hatching egg saves, saves relative humidity and be set as 75%~80%;
Preservation ten days or more hatching eggs must carry out every 5 days 1 time pre-temperature, to improve the hatching rate of hatching egg before entering to incubate.
This is suitable for the method that northern birds hatching egg can extend shelf life and improve hatching rate, also meet condition, it is described
Specifically: the hatching egg of selection is same duck group, same batch, same age in days, and the holding time is 11 days~15 days.
This is suitable for the method that northern birds hatching egg can extend shelf life and improve hatching rate, also meets condition, described
Specifically: hatching egg carries out the 3rd disinfection in incubator before entering to incubate.
This is suitable for the method that northern birds hatching egg can extend shelf life and improve hatching rate, also meets condition, described
Specifically: hatching egg library carries out spraying disinfection to hatching egg library ground every progress fumigation in 7 days, every 2 days.
This is suitable for the method that northern birds hatching egg can extend shelf life and improve hatching rate, also meets condition, described
Specifically: hatching egg library disinfecting drug is cleared away with bacterium.
This is suitable for the method that northern birds hatching egg can extend shelf life and improve hatching rate, also meets condition, described
Specifically: the every 5 days 1 time pre-temperature method of hatching egg, pre-temperature temperature are 25 DEG C~35 DEG C.
This is suitable for the method that northern birds hatching egg can extend shelf life and improve hatching rate, also meets condition, described
Specifically: each pre-heating time of hatching egg is 1 hour.
To verify a kind of side that can extend shelf life and improve hatching rate suitable for northern birds hatching egg provided by the invention
Method validity, spy do following comparative experiments:
The preservation condition of 1 hatching egg
The test of 1.1 hatching egg storage temperatures
Use storage temperature be respectively 15 DEG C~18 DEG C and 9~12 DEG C, relative humidity for 60%~70% preservation 15
It hatching egg is tested.Its test result is as shown in table 1:
1 hatching egg of table saves different storage temperatures test in 15 days
As seen from the experiment, under identical relative humidity Conservation environment, it is stored in 9 DEG C~12 DEG C of hatching of breeding eggs rate
Hatching of breeding eggs rate than being stored in 5 DEG C~18 DEG C is high by 4.09%, illustrates that 9 DEG C~12 DEG C of storage temperature is more suitable for improving and saves ten
The hatching rate of its hatching egg, more conducively preservation hatching egg.However, it is observed that it is found that eggshell inner membrance is relatively dry, just the case where just young bird broken shell
Raw young bird hair color is shallower.Given this test situation, and carried out hatching egg and saved the test for improving humidity.
1.2 hatching eggs save humid test
This test carries out under conditions of storage temperature is 9 DEG C~12 DEG C, relative humidity is 75%~80%.With relatively wet
Degree 65~70% is control group, and it is test group that relative humidity, which is 75%~80%,.Test result is as shown in table 2.
2 hatching egg of table saves different humidity test in 15 days
Test result shows that test group hatching rate is higher by control group 5%, and day old chick hair color is normal, and eggshell is wet normal.
In order to inquire into this preservation condition if appropriate for 11 to 14 days hatching eggs are saved, respectively to be 9 DEG C in storage temperature~
12 DEG C, in the environment of relative humidity is 75%~80%, save 11 days, 13 days, 15 days hatching eggs tested, to save 15 days
Hatching egg be control group, test result is as shown in table 3.
3 hatching egg of table saves 11 days, 13 days, the test of 15 days different humidities
Through statistical test, each group difference is not significant.Test result show temperature be 9 DEG C~12 DEG C, humidity be 75~
80% Conservation environment is suitble to save 11 days~15 days hatching eggs.
The sterilizing part of 2 hatching eggs
The reason of 2.1 hatching eggs sterilize
Hatching egg is from female bird genital tract output or touches unclean laying nest, padding etc., tends to by environment and sky
The pollution of bacterium in gas.According to surveying and determination, just giving birth to the egg surface come has 100~300 bacteriums, can be bred after 1 hour to 4000~
5000.Although hatching egg has gelatinous layer, eggshell and inner membrance and the road albumin Deng Ji natural barrier from eggshell structure, this
A little barriers are limited to the prevention ability of germ.Over time, eggshell is contaminated, wet especially when eggshell surface temperature height
When spending big, these bacteriums (especially mould) are easy to breed on shell, break through in protective barrier intrusion egg, some germs can be straight
Connecing leads to embryonic death, smelly rotten corruption;Some germs then lead to disease young bird, weak young bird, seriously affect hatching rate and duckling survival rate.More
The serious is pollution incubator and apparatus, longitudinal to infect various diseases, endanger very big.Therefore, hatching egg needs before saving to hatching egg
Timely sterilized.
2.2 hatching egg sterilization methods
Disinfectant program of the invention is to carry out first time disinfection at once after hatching egg collection, then carries out hatching egg in selection room
Selection, qualified hatching egg is carried out second and is sterilized, hatching egg in incubator carries out third time disinfection before entering to incubate.
In addition, determining disappear stifling every 7 days to hatching egg library by the long term monitoring of the mushroom laboratory indexes to storage environment
It is malicious primary, it is every 2 days that hatching egg library ground spraying disinfection is primary.Drug used is cleared away with bacterium.
The pre-temperature of 3 hatching eggs is tested
The reason of 3.1 hatching egg pre-temperature
Due to the characteristic of avian embryonic, in the environment lower than 21 DEG C in a dormant state.Pre-temperature is exactly with raising temperature
To activate embryo in a dormant state to enter developmental condition.
Hatching egg can achieve following three purposes by preheating before entering to incubate:
(1) viability of embryo can be enhanced.It is stored in hatching egg library before entering to incubate because of hatching egg, temperature is lower, humidity is larger
Environmental condition under, embryo in a dormant state, if hatching egg is directly incubated into people by hatching egg library storage temperature (15 DEG C~18 DEG C)
It is too big to the stimulation of embryo in the hot environment of change (37 DEG C~39.5 DEG C).By preheating, make the temperature of hatching egg by low temperature slowly
Into high temperature, embryo is made gradually " to revive " by stationary state, is conducive to enhance embryonic itality, promotes embryonic development.
(2) be conducive to the stabilization of incubator temperature.People's hatch machine is filled after hatching egg is preheated, it is ensured that continuous incubation temperature
Stability avoids hatch machine temperature sharp fall, influences the normal development of the embryo of other batches.
(3) be conducive to fumigation.Hatching egg by preheating, can avoid hatching egg sweating water pearl, be conducive to hatching egg into
Row fumigation.
As the holding time of hatching egg extends, hatching rate is gradually decreased.Eggshell surface known to analysis by disinfection still has
In a large amount of bacterial reproduction intrusion hatching egg, embryo is made to be led to death by the infringement of pathogenic microorganism, so as to cause hatching rate drop
It is low.For this purpose, taking the practicability of following tests verifying inventive method.
The test of 3.2 hatching egg pre-temperatures
Change test and be divided into 3 groups, the storage number of days of hatching egg is 15 days, and the temperature of pre-temperature is set as 35 DEG C, every 3 days, 5 days, 7
Its each pre-temperature is primary.
This test why by test group be divided into 3 days, 5 days, 7 days each pre-temperatures that carry out it is primary, be due to according to different guarantors
Deposit the hatching egg of time it is found that save 2 days~3 days hatching eggs carry out it is more into incubating, but also can hatching egg save 5 days~7 days into
Row enters to incubate, and the hatching egg incubated is entered within the above period after carrying out conventional pre-temperature activation embryo, discovery hatching of breeding eggs result is normal.
Therefore 3 days, 5 days, the primary test of 7 days each pre-temperatures are devised on this basis.But the temperature of pre-temperature is than conventional pre-temperature 22 DEG C~26
DEG C temperature it is high, be set as 35 DEG C.
Main operation link is as follows:
(1) by hatching egg library Temperature and Humidity Control at 9 DEG C~12 DEG C of temperature, relative humidity 75%~80%.
(2) to shift incubator pre-temperature onto when hatching egg is saved in 3 days, 5 days, 7 days primary.
(3) egg vehicle is directly pushed into incubator from hatching egg library when pre-temperature, incubator temperature setting is 35 DEG C.It is shown in machine
When temperature reaches 35 DEG C, need to open machine door, with temperature measurer measurement upper, middle and lower, the eggshell of the hatching egg of left and right different location
Surface temperature confirms that the eggshell surface temperature of hatching egg has reached 34 DEG C, then maintains 1h, then pulls out hatching egg out of machine, puts
Hatching egg library is pushed into after cooling 1h~2h between hatching.Since the time only has 4h during pre-temperature, therefore need not egg-turning.
Booting pre-temperature about 4 hours every time, makes egg table temperature reach 34 DEG C, is monitored with infrared thermometer.Hatching egg library temperature
Control realizes that control temperature, humidity generally use ground watering or humidifier adjusting dry and wet temp by temperature controller using refrigeration machine
Degree meter monitoring control.
Temperature is set as 35 DEG C by this test, foundation are as follows: 35 DEG C be embryo's normal development lower limit, embryo at a temperature of this
Viability be embryo revival after maximum value.In view of having reached incubation temperature due to preheating temperature, time slightly long embryo is just
It will do it cell division proliferation, will cause developmental embryonic death when cooling is saved again.So to save the time,
Working efficiency is improved, the activationary time of embryo is determined as 1 hour, and to cool down 1 in the environment of (22 DEG C~24 DEG C) between hatching
Hour~2 hours, in case the environment that embryo enters directly into 9 DEG C~12 DEG C by 35 DEG C is unfavorable to embryo.Then it is pushed into hatching egg again
Library makes embryo enter dormant state again.The present invention enters machine of incubating, the difference of eggshell surface temperature and set temperature about at 1 DEG C, when
When set temperature is 35 DEG C, egg temperature is about 34 DEG C at this time, by measuring eggshell surface temperature, can more accurately hold hatching to temperature.
Test result is as shown in table 4:
4 hatching egg of table saves 15 days different pre-heating time tables
Prove that primary than the 3 days primary chickling-surviving rates of pre-temperature of chickling-surviving rate of every 5 days pre-temperatures were high by 3.84%, than 7 days by test
The primary chickling-surviving rate of pre-temperature is high by 6.72%, it follows that the hatching rate highest that every 5 days pre-temperatures are primary, close to incubating for short-term preservation
Rate.
It is primary using every 5 days pre-temperatures to keep test more scientific, it is respectively 35 DEG C, 30 DEG C, 25 DEG C in preheating temperature
Condition is tested.With 35 DEG C of preheating temperature for control group, preheating time is 1 hour, other operation link each groups are all the same.
Test result is as shown in table 5:
The different preheating temperatures of table 5 test table
Through statistical test, each group difference is not significant.Illustrate that the hatching egg holding time at 11 days or more, carries out every 5 days 1
Secondary pre-temperature can effectively improve hatching of breeding eggs rate.
In nearly biennial production, when hatching egg cannot enter to incubate on time for some reason, the holding time used always at 11 days~15 days
Every 5 days pre-temperatures are primary, 9 DEG C~12 DEG C of storage temperature, relative humidity 75%~80% storage method, achieve good hatching
Effect has reached the hatching rate of short-term preservation 83%~84%.
The above embodiment is a preferred embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, other any changes, modifications, substitutions, combinations, simplifications made without departing from the spirit and principles of the present invention,
It should be equivalent substitute mode, be included within the scope of the present invention.
Claims (6)
1. a kind of method that can extend shelf life and improve hatching rate suitable for northern birds hatching egg, which is characterized in that including such as
Lower step:
S1 hatching egg is collected;
S2 hatching egg carries out the 1st hatching egg disinfection immediately after collecting;
Hatching egg after S3 disinfection enters selection room and selects qualified hatching egg;
S4 carries out the 2nd disinfection to qualified hatching egg;
Hatching egg after S5 disinfection is sent into the storage of hatching egg library;During hatching egg library stores, temperature setting is 9 DEG C~12 DEG C;Relative humidity
It is set as 75%~80%;
The hatching of S6 incubator: the storage of hatching egg library ten days or more hatching eggs, hatching egg must carry out every 5 days 1 time pre-temperature before entering to incubate.
2. the method as described in claim 1 for being suitable for northern birds hatching egg and can extending shelf life and improve hatching rate, special
Sign is that the hatching egg is same duck group, same batch, same age in days, and the holding time of hatching egg is 11 days~15 days.
3. the method as described in claim 1 for being suitable for northern birds hatching egg and can extending shelf life and improve hatching rate, special
Sign is that hatching egg library described in step S5 carries out spraying disinfection to hatching egg library ground every progress fumigation in 7 days, every 2 days.
4. the method as described in claim 1 for being suitable for northern birds hatching egg and can extending shelf life and improve hatching rate, special
Sign is that hatching egg described in the step S6 carries out the 3rd disinfection in incubator before entering to incubate.
5. the method as described in claim 1 for being suitable for northern birds hatching egg and can extending shelf life and improve hatching rate, special
Sign is that the every 5 days 1 time pre-temperature method of hatching egg described in step S6, hatching egg pre-temperature temperature is 25 DEG C~35 DEG C.
6. the method as described in claim 1 for being suitable for northern birds hatching egg and can extending shelf life and improve hatching rate, special
Sign is that the every 5 days 1 time pre-temperature method of hatching egg described in step S6, each pre-heating time of hatching egg is 1 hour.
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