CN109477080A - polX家族的DNA聚合酶的变体 - Google Patents

polX家族的DNA聚合酶的变体 Download PDF

Info

Publication number
CN109477080A
CN109477080A CN201780037427.0A CN201780037427A CN109477080A CN 109477080 A CN109477080 A CN 109477080A CN 201780037427 A CN201780037427 A CN 201780037427A CN 109477080 A CN109477080 A CN 109477080A
Authority
CN
China
Prior art keywords
variant
leu
residue
glu
arg
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201780037427.0A
Other languages
English (en)
Chinese (zh)
Inventor
托马斯·伊贝尔特
马克·德拉吕
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Centre National de la Recherche Scientifique CNRS
DNA Script SAS
Institut Pasteur
Original Assignee
Centre National de la Recherche Scientifique CNRS
DNA Script SAS
Institut Pasteur
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centre National de la Recherche Scientifique CNRS, DNA Script SAS, Institut Pasteur filed Critical Centre National de la Recherche Scientifique CNRS
Publication of CN109477080A publication Critical patent/CN109477080A/zh
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1264—DNA nucleotidylexotransferase (2.7.7.31), i.e. terminal nucleotidyl transferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07031—DNA nucleotidylexotransferase (2.7.7.31), i.e. terminal deoxynucleotidyl transferase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201780037427.0A 2016-06-14 2017-06-13 polX家族的DNA聚合酶的变体 Pending CN109477080A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR1655475 2016-06-14
FR1655475A FR3052462A1 (fr) 2016-06-14 2016-06-14 Variants d'une adn polymerase de la famille polx
PCT/FR2017/051519 WO2017216472A2 (fr) 2016-06-14 2017-06-13 Variants d'une adn polymérase de la famille polx

Publications (1)

Publication Number Publication Date
CN109477080A true CN109477080A (zh) 2019-03-15

Family

ID=57348782

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201780037427.0A Pending CN109477080A (zh) 2016-06-14 2017-06-13 polX家族的DNA聚合酶的变体

Country Status (11)

Country Link
US (3) US20200002690A1 (https=)
EP (1) EP3469075A2 (https=)
JP (2) JP7118510B2 (https=)
KR (1) KR102522263B1 (https=)
CN (1) CN109477080A (https=)
AU (2) AU2017286477C1 (https=)
CA (1) CA3024184A1 (https=)
FR (2) FR3052462A1 (https=)
IL (1) IL263503B2 (https=)
SG (2) SG11201809961TA (https=)
WO (1) WO2017216472A2 (https=)

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110331136A (zh) * 2019-09-05 2019-10-15 中国科学院天津工业生物技术研究所 一种末端脱氧核糖核苷转移酶变体及其应用
CN112322715A (zh) * 2020-11-17 2021-02-05 清华大学 一种核酸测序方法
CN113302289A (zh) * 2018-11-14 2021-08-24 Dna斯克瑞普特公司 末端脱氧核苷酸转移酶变体及其用途
CN114207140A (zh) * 2019-05-28 2022-03-18 Dna斯克瑞普特公司 末端脱氧核苷酸转移酶变体及其用途
CN114787346A (zh) * 2019-12-12 2022-07-22 Dna斯克瑞普特公司 用于多核苷酸无模板酶学合成的嵌合末端脱氧核苷酸转移酶
WO2023143123A1 (zh) * 2022-01-28 2023-08-03 中国科学院天津工业生物技术研究所 可控合成单链dna的末端转移酶变体及应用
WO2025140014A1 (zh) * 2023-12-26 2025-07-03 常州新一产生命科技有限公司 末端脱氧核苷酸转移酶突变体在合成核酸中的应用

Families Citing this family (62)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10774316B2 (en) * 2014-10-20 2020-09-15 Molecular Assemblies, Inc. Modified template-independent enzymes for polydeoxynucleotide synthesis
AU2018272302A1 (en) * 2017-05-26 2019-12-19 Nuclera Nucleics Ltd Use of terminal transferase enzyme in nucleic acid synthesis
US11390856B2 (en) 2017-08-07 2022-07-19 Dna Script Variants of family a DNA polymerase and uses thereof
US10752887B2 (en) 2018-01-08 2020-08-25 Dna Script Variants of terminal deoxynucleotidyl transferase and uses thereof
US10435676B2 (en) 2018-01-08 2019-10-08 Dna Script Variants of terminal deoxynucleotidyl transferase and uses thereof
EP3826762A1 (en) 2018-07-23 2021-06-02 DNA Script Massively parallel enzymatic synthesis of nucleic acid strands
SG11202105441WA (en) 2018-12-13 2021-06-29 Dna Script Direct oligonucleotide synthesis on cells and biomolecules
WO2020141143A1 (en) 2019-01-03 2020-07-09 Dna Script One pot synthesis of sets of oligonucleotides
GB201901501D0 (en) * 2019-02-04 2019-03-27 Nuclera Nucleics Ltd Modified terminal deoxynucleotidyl transferase (TdT) enzymes
WO2020165137A1 (en) 2019-02-12 2020-08-20 Dna Script Efficient product cleavage in template-free enzymatic synthesis of polynucleotides.
EP3744854A1 (en) * 2019-05-28 2020-12-02 DNA Script Variants of terminal deoxynucleotidyl transferase and uses thereof
WO2021018919A1 (en) 2019-07-30 2021-02-04 Dna Script Template-free enzymatic synthesis of polynucleotides using poly(a) and poly(u) polymerases
JP2022543568A (ja) 2019-08-01 2022-10-13 ディーエヌエー スクリプト ポリヌクレオチドの鋳型なしの酵素による合成における長い配列の収量の増加
CN114555818A (zh) 2019-09-09 2022-05-27 Dna斯克瑞普特公司 使用光可切割连接的无模板酶促多核苷酸合成
WO2021058438A1 (en) 2019-09-23 2021-04-01 Dna Script Increasing long-sequence yields in template-free enzymatic synthesis of polynucleotides
AU2020384271A1 (en) 2019-11-13 2022-06-23 Dna Script High efficiency template-free enzymatic synthesis of polynucleotides
WO2021122539A1 (en) 2019-12-16 2021-06-24 Dna Script Template-free enzymatic polynucleotide synthesis using dismutationless terminal deoxynucleotidyl transferase variants
EP4110940B1 (en) 2020-02-25 2024-03-27 DNA Script Method and apparatus for enzymatic synthesis of polynucleotides
US20230159903A1 (en) 2020-04-20 2023-05-25 Dna Script Terminal Deoxynucleotidyl Transferase Variants and Uses Thereof
JP7828081B2 (ja) * 2020-05-12 2026-03-11 ナショナル ユニヴァーシティー オブ シンガポール 熱安定性末端デオキシヌクレオチジルトランスフェラーゼ
EP4150065A2 (en) 2020-05-12 2023-03-22 Illumina Inc Generating nucleic acids with modified bases using recombinant terminal deoxynucleotidyl transferase
CN116075593A (zh) * 2020-06-03 2023-05-05 分子组装公司 用于聚脱氧核苷酸合成的经修饰的非模板依赖性酶
CN116249782A (zh) 2020-06-12 2023-06-09 辛希克斯 使用热稳定性酶从头开始的不依赖于模板的核酸合成
WO2021254934A1 (en) 2020-06-16 2021-12-23 Dna Script Systems, apparatus and kits for enzymatic polynucleotide synthesis
WO2022013094A1 (en) 2020-07-15 2022-01-20 Dna Script Massively parallel enzymatic synthesis of polynucleotides
US11993801B2 (en) 2020-07-21 2024-05-28 Illumina Singapore Pte. Ltd. Base-modified nucleotides as substrates for TdT-based enzymatic nucleic acid synthesis
WO2022029427A1 (en) * 2020-08-04 2022-02-10 Nuclera Nucleics Ltd MODIFIED TERMINAL DEOXYNUCLEOTIDYL TRANSFERASE (TdT) ENZYMES
GB2598152B (en) * 2020-08-21 2025-04-16 Nuclera Ltd Modified terminal deoxynucleotidyl transferase (TdT) enzymes
AU2021347452A1 (en) 2020-09-22 2023-04-27 Dna Script Stabilized n-terminally truncated terminal deoxynucleotidyl transferase variants and uses thereof
EP4237573A1 (en) 2020-10-29 2023-09-06 DNA Script Enzymatic synthesis of polynucleotide probes
US12391970B2 (en) 2020-11-11 2025-08-19 Microsoft Technology Licensing, Llc Spatial control of polynucleotide synthesis by strand capping
FR3121442A1 (fr) 2021-04-02 2022-10-07 Dna Script Procédés et trousses pour la synthèse enzymatique de polynucléotides pouvant former des g4
NL2032097B1 (en) 2021-06-10 2024-03-29 Dna Script Enzymatic synthesis of polynucleotides using 3'-o-amino-2'-deoxyribonucleoside triphosphate monomers
EP4392554A1 (en) 2021-08-23 2024-07-03 DNA Script Method for generating double-stranded nucleic acid
US20240299945A1 (en) 2021-09-03 2024-09-12 Elegen Corporation Multi-way bead-sorting devices, systems, and methods of use thereof using pressure sources
CA3234383A1 (en) 2021-10-15 2023-04-27 Codexis, Inc. Engineered terminal deoxynucleotidyl transferase variants
WO2023083997A2 (en) 2021-11-10 2023-05-19 Dna Script Novel terminal deoxynucleotidyl
EP4430176A2 (en) 2021-11-10 2024-09-18 DNA Script Novel terminal deoxynucleotidyl transferase (tdt) variants
WO2023170286A2 (en) 2022-03-11 2023-09-14 Dna Script Alignment post and secure mechanism for enzymatic polynucleotide synthesis
US20250205700A1 (en) 2022-03-11 2025-06-26 Dna Script Automation station for enzymatic polynucleotide synthesis
WO2023170258A1 (en) 2022-03-11 2023-09-14 Dna Script Apparatus for enzymatic synthesis of a plurality of polynucleotides comprising a condensation trap
WO2023170259A1 (en) 2022-03-11 2023-09-14 Dna Script Modular accessory rack
WO2023240202A2 (en) * 2022-06-08 2023-12-14 Ansa Biotechnologies, Inc. Modified terminal deoxynucleotidyl transferase polymerases
EP4555083A1 (en) 2022-07-13 2025-05-21 DNA Script Method of purifying solution comprising polynucleotide
EP4630540A1 (en) 2022-12-05 2025-10-15 DNA Script Variants of poly(a) polymerase and uses thereof
CN120359296A (zh) 2022-12-09 2025-07-22 Dna斯克瑞普特公司 体外酶促rna合成
WO2024141628A1 (en) 2022-12-31 2024-07-04 Dna Script Variable viscosity inks for inkjet delivery of enzyme reagents
WO2024153642A1 (en) 2023-01-16 2024-07-25 Dna Script Scarless template-free enzymatic synthesis of polynucleotides
WO2024153643A1 (en) 2023-01-16 2024-07-25 Dna Script Inkjet-assisted enzymatic nucleic acid synthesis
CN120530202A (zh) 2023-01-26 2025-08-22 Dna斯克瑞普特公司 多核苷酸探针的酶促合成
WO2024227846A2 (en) 2023-05-03 2024-11-07 Dna Script Reagent used in deprotection of 3'-o-amino polynucleotides
WO2024241324A1 (en) * 2023-05-24 2024-11-28 Scintium Ltd Method for training an artificial intelligence (ai) model and generating a 3d computer-aided design (cad) and system therefor
WO2024256604A1 (en) 2023-06-15 2024-12-19 Dna Script Salt addition during enzymatic polynucleotide synthesis
EP4735621A1 (en) 2023-06-30 2026-05-06 DNA Script Nucleic acid synthesis on reusable support
CN121532522A (zh) 2023-07-27 2026-02-13 Dna斯克瑞普特公司 用于产生多个多核苷酸的方法
WO2025088024A1 (en) 2023-10-24 2025-05-01 Dna Script Novel terminal deoxynucleotidyl transferase (tdt) variants and uses thereof
WO2025093659A1 (en) 2023-11-04 2025-05-08 Dna Script 3'-o-aminooxymethyl blocked nucleotides and use thereof in enzymatic synthesis of polynucleotides
WO2025155239A1 (en) * 2024-01-17 2025-07-24 Agency For Science, Technology And Research X family dna polymerases and uses thereof
EP4653525A1 (en) * 2024-05-21 2025-11-26 Uab "Biomatter Designs" Engineered terminal deoxynucleotidyl transferase polymerases
WO2026008717A2 (en) 2024-07-02 2026-01-08 Dna Script Method for enzymatic synthesis of polynucleotides
WO2026008711A2 (en) 2024-07-02 2026-01-08 Dna Script Filtering device and method for polynucleotide enzymatic synthesis
WO2026017834A1 (en) 2024-07-19 2026-01-22 Dna Script Inkjet-assisted enzymatic nucleic acid synthesis and cleavage

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102257136A (zh) * 2008-11-03 2011-11-23 卡帕生物系统 嵌合dna聚合酶
US20160108382A1 (en) * 2014-10-20 2016-04-21 Molecular Assemblies, Inc. Modified template-independent enzymes for polydeoxynucleotide synthesis

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5004689A (en) 1982-02-22 1991-04-02 Biogen, Massachusetts DNA sequences, recombinant DNA molecules and processes for producing human gamma interferon-like polypeptides in high yields
IL71691A (en) 1984-04-27 1991-04-15 Yeda Res & Dev Production of interferon-ypsilon
US5047335A (en) 1988-12-21 1991-09-10 The Regents Of The University Of Calif. Process for controlling intracellular glycosylation of proteins
BG52073B2 (en) 1990-01-24 1996-04-30 Inst Molekuljarna Biolog Method for the preparation of recombinant human noncystein -interferon, free of n-end methionine
US6812339B1 (en) * 2000-09-08 2004-11-02 Applera Corporation Polymorphisms in known genes associated with human disease, methods of detection and uses thereof
CA2864394C (en) * 2011-03-02 2021-10-19 Jack Roth A method of predicting a response to a tusc2 therapy
EP3080158A4 (en) * 2013-12-09 2018-03-21 Adimab, LLC Polyclonal mixtures of antibodies, and methods of making and using them
FR3020071B1 (fr) 2014-04-17 2017-12-22 Dna Script Procede de synthese d'acides nucleiques, notamment d'acides nucleiques de grande longueur, utilisation du procede et kit pour la mise en œuvre du procede
FR3025201B1 (fr) 2014-09-02 2018-10-12 Dna Script Nucleotides modifies pour la synthese d'acides nucleiques, un kit renfermant de tels nucleotides et leur utilisation pour la production de genes ou sequences d'acides nucleiques synthetiques

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102257136A (zh) * 2008-11-03 2011-11-23 卡帕生物系统 嵌合dna聚合酶
US20160108382A1 (en) * 2014-10-20 2016-04-21 Molecular Assemblies, Inc. Modified template-independent enzymes for polydeoxynucleotide synthesis
WO2016064880A1 (en) * 2014-10-20 2016-04-28 Molecular Assemblies, Inc. Modified template-independent enzymes for polydeoxynucleotide systhesis

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
B YANG, K N GATHY, M S COLEMAN: ""Mutational analysis of residues in the nucleotide binding domain of human terminal deoxynucleotidyl transferase"", 《J BIOL CHEM》 *
M. DELARUE, ET AL.: ""Crystal structures of a template-independent DNA polymerase: murine terminal deoxynucleotidyltransferase"", 《EMBO J. 》 *
SAID AOUFOUCHI, ET AL.: ""Two novel human and mouse DNA polymerases of the polX family"", 《NUCLEIC ACIDS RES. 》 *

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113302289A (zh) * 2018-11-14 2021-08-24 Dna斯克瑞普特公司 末端脱氧核苷酸转移酶变体及其用途
CN114207140A (zh) * 2019-05-28 2022-03-18 Dna斯克瑞普特公司 末端脱氧核苷酸转移酶变体及其用途
CN110331136A (zh) * 2019-09-05 2019-10-15 中国科学院天津工业生物技术研究所 一种末端脱氧核糖核苷转移酶变体及其应用
CN110331136B (zh) * 2019-09-05 2019-12-24 中国科学院天津工业生物技术研究所 一种末端脱氧核糖核苷转移酶变体及其应用
CN114787346A (zh) * 2019-12-12 2022-07-22 Dna斯克瑞普特公司 用于多核苷酸无模板酶学合成的嵌合末端脱氧核苷酸转移酶
CN112322715A (zh) * 2020-11-17 2021-02-05 清华大学 一种核酸测序方法
WO2022105640A1 (zh) * 2020-11-17 2022-05-27 清华大学 一种核酸测序方法
WO2023143123A1 (zh) * 2022-01-28 2023-08-03 中国科学院天津工业生物技术研究所 可控合成单链dna的末端转移酶变体及应用
WO2025140014A1 (zh) * 2023-12-26 2025-07-03 常州新一产生命科技有限公司 末端脱氧核苷酸转移酶突变体在合成核酸中的应用
WO2025137872A1 (zh) * 2023-12-26 2025-07-03 华大工程生物学长荡湖研究所 一种末端脱氧核糖核苷转移酶突变体及其制备方法和应用

Also Published As

Publication number Publication date
IL263503A (en) 2019-02-03
IL263503B1 (en) 2024-12-01
AU2023282219A1 (en) 2024-01-18
US20230167421A1 (en) 2023-06-01
SG10202009688QA (en) 2020-11-27
IL263503B2 (en) 2025-04-01
JP7118510B2 (ja) 2022-08-16
CA3024184A1 (en) 2017-12-21
WO2017216472A3 (fr) 2018-03-15
FR3053699A1 (fr) 2018-01-12
FR3052462A1 (fr) 2017-12-15
US20200002690A1 (en) 2020-01-02
AU2017286477B2 (en) 2023-09-14
SG11201809961TA (en) 2018-12-28
JP2022137127A (ja) 2022-09-21
AU2017286477C1 (en) 2023-12-21
KR20190024883A (ko) 2019-03-08
EP3469075A2 (fr) 2019-04-17
JP2019517799A (ja) 2019-06-27
AU2017286477A1 (en) 2018-12-06
FR3053699B1 (fr) 2021-04-02
KR102522263B1 (ko) 2023-04-18
WO2017216472A2 (fr) 2017-12-21
US20240124855A1 (en) 2024-04-18

Similar Documents

Publication Publication Date Title
CN109477080A (zh) polX家族的DNA聚合酶的变体
JP7292442B2 (ja) 改変アミノアシルｔＲＮＡ合成酵素およびその用途
CN110551700B (zh) Adh蛋白家族突变体及其应用
CN108239633B (zh) 一种催化活性得到提高的d-阿洛酮糖-3-差向异构酶的突变体及其应用
CN104379748B (zh) L-核酸的酶促合成
US11390856B2 (en) Variants of family a DNA polymerase and uses thereof
KR20160079126A (ko) 효소를 통한 l-핵산의 합성
CN111254134A (zh) 腈基水解酶突变体及其在(s)-单腈单酸合成中的应用
KR101998477B1 (ko) 클로스트리디움 스터코라리움 유래 엘-람노스 이성화효소 변이체 및 이를 이용한 알룰로스로부터 알로스의 생산 방법
CN109207448B (zh) 新型黄酮异戊烯基转移酶及其应用
CN116334153A (zh) 还原胺化酶及其制法和应用
CN108531527A (zh) 一种d-阿洛酮糖-3-差向异构酶的应用
CN111575251B (zh) 一种用于达托霉素生物合成的dptC1突变体的构建
CN113025596A (zh) 耐甲醇脂肪酶
JP2010505410A (ja) 変異体ｄｎａポリメラーゼ及びそれらの遺伝子
CN105779418B (zh) 一种新型的脂肪酶
CN105779413B (zh) 一种新型的脂肪酶
CN100537754C (zh) 一种s-腺苷甲硫氨酸生产菌株的构建及大规模发酵
CN113046333A (zh) 黄酮-8-羟化酶及其在8-羟基黄酮化合物合成中的应用
CN105779416B (zh) 一种新型的脂肪酶
CN105779414B (zh) 一种新型的脂肪酶
CN105779415B (zh) 一种新型的脂肪酶
WO2002042323A2 (en) Fluorescent proteins
CN118048335A (zh) 一种参与桂叶苷A生物合成的PbAT1蛋白及其编码基因与应用

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination