CN109468348A - 己二酸酯或硫代酯合成 - Google Patents

己二酸酯或硫代酯合成 Download PDF

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CN109468348A
CN109468348A CN201810937045.XA CN201810937045A CN109468348A CN 109468348 A CN109468348 A CN 109468348A CN 201810937045 A CN201810937045 A CN 201810937045A CN 109468348 A CN109468348 A CN 109468348A
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吴亮
埃克斯勒·克里斯多佛·特里弗泽
威尔德曼 斯蒂法恩·马莉亚·安德勒·德
德 勃戈 马尔科·亚历山大·范
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Abstract

本发明涉及己二酸酯或硫代酯合成。本发明涉及用于制备己二酸酯或硫代酯的方法。本发明还涉及从所述酯或硫代酯制备己二酸的方法。本发明还提供了用于制备所述酯或硫代酯中间产物的大量方法。本发明还涉及用于制备6‑氨基己酸(6‑ACA)的方法，制备5‑甲酰基戊酸(5‑FVA)的方法和制备己内酰胺的方法。此外，本发明还涉及在根据本发明的方法中使用的宿主细胞。

Description

己二酸酯或硫代酯合成
本申请是申请号为200980116934.9的中国专利申请的分案申请，原申请是申请日为2009年3月11日的国际申请PCT/NL2009/050115的中国国家阶段申请。
技术领域
本发明涉及制备己二酸酯或硫代酯的方法。本发明还涉及从所述酯或硫代酯制备己二酸的方法。本发明还提供了用于制备所述酯或硫代酯的中间产物的大量方法。本发明还涉及用于制备6-氨基己酸(6-ACA)的方法，制备5-甲酰基戊酸(5-FVA)的方法和制备己内酰胺的方法。本发明还涉及在根据本发明的方法中使用的宿主细胞。
背景技术
己二酸(肥酸)等等被用于生产聚酰胺。另外，己二酸酯可在增塑剂、润滑剂、溶剂和多种聚氨酯树脂中使用。己二酸的其它用途是用作食物酸化剂，应用于粘附剂、杀虫剂、制革剂(tanning)和染料中。已知的制备方法包括用硝酸氧化环己醇或环己酮或其混合物(KA油)。
己内酰胺是一种内酰胺，其也可以被用于生产聚酰胺，例如尼龙-6或己内酰胺-十二内酰胺共聚物(尼龙-6,12)。由大量化学品制备己内酰胺的多种方式是本领域已知的，并且包括由环己酮、甲苯、苯酚、环己醇、苯或环己烷制备己内酰胺。
用于制备己二酸或己内酰胺的中间产物化合物如环己醇、环己酮或苯酚通常得自矿物油。考虑到使用更加可持续的技术来制备材料的日益增长的需要，期望提供一种方法，其中由能够从生物可再生来源获得的中间产物化合物或至少由使用生物化学方法被转化成己二酸或己内酰胺的中间产物来制备己二酸或己内酰胺。
US 5,487,987中描述了用于生产己二酸的一种方法，其中利用细菌细胞，所述细菌细胞中碳源被细菌细胞的一般通路或芳香氨基酸生物合成中的酶转化成3-脱氢莽草酸酯，从而通过3-脱氢莽草酸酯的生物催化转化来生产顺式,顺式粘康酸。顺式,顺式粘康酸之后被(使用铂催化剂)化学还原，生产己二酸。因此，最终步骤要求化学催化。本发明人还考虑到细菌细胞中形成的芳香族中间产物对细胞可能是有毒的，很可能需要它们在体内以及细胞培养物中浓度较低。
已知由6-氨基己酸(6-ACA)制备己内酰胺，例如如US-A 6,194,572中所述。如WO2005/068643中所公开的，可以在存在具有α,β-烯酸酯还原酶活性的酶时，通过转化6-氨基己-2-稀酸(6-AHEA)来生物化学地制备6-ACA。可以例如生物化学地或通过纯化学合成，由赖氨酸制备6-AHEA。尽管可以通过WO 2005/068643中公开的方法，通过6-AHEA的还原制备6-ACA，但是本发明人发现在还原反应条件下，6-AHEA可自发并且基本不可逆地环化形成不想要的副产物，特别是β-高脯氨酸。所述环化可以是6-ACA生产中的瓶颈，并且导致产率的可观损失。
发明内容
本发明的一个目的是提供用于制备己二酸或己内酰胺(尤其可以被用于制备聚酰胺)或己二酸或己内酰胺的中间产物化合物的新颖方法，所述方法可以作为已知方法的替代方式。
本发明的又一个目的是提供克服一种或多种上述缺点的新颖方法。
根据本发明可解决的一个或多个其它目的可根据下文的说明书得到。
本发明的发明人实现了能够从琥珀酸(或其酯或硫代酯)生产己二酸(或其酯或硫代酯)。具体地，本发明人的结论是可以通过一系列特定的反应(例如与活细胞中的反转β-氧化和脂肪酸生物合成相似)，从琥珀酸(硫代)酯和乙酸(硫代)酯制备己二酸(硫代)酯，如图2中所示。本文中，每个R独立地表示(促进反应的)活化基团，例如如下文所述。每个X独立地表示S或O。ED/EDH2例示了被氧化/被还原的电子供体，例如NAD/NADH、NADP/NADPH、FAD/FADH2或被氧化的铁氧还蛋白/被还原的铁氧还蛋白。电子的实际转移可直接发生，或者可由中间体电子运载体如辅酶或电子转移黄素蛋白(ETF)来介导。Y-NH2是指氨基供体，例如如下文所述。
因此本发明人得出结论，可通过反应级联放大，从琥珀酸(或其酯或硫代酯)和乙酸(或其酯或硫代酯)生物催化性制备己二酸(或其酯或硫代酯)。另外，他们实现了可以将己二酸生物催化地转化成6-ACA制备的中间产物——5-甲酰戊酸(“5-FVA”，5-甲酰缬草酸)，并且对所述转化而言可使用特定的生物催化剂。
因此，本发明涉及通过大量反应，由琥珀酸酯或硫代酯制备己二酸酯或硫代酯的方法，其中至少一种所述反应由生物催化剂催化。
具体地，本发明涉及用于制备己二酸酯或己二酸硫代酯的方法，所述方法包括在存在生物催化剂时，将2,3-脱氢己二酸(IUPAC名称5-羧基-2-戊酸)酯或2,3-脱氢己二酸硫代酯转化成己二酸酯或硫代酯。
除非另有说明，在本文中提到羧酸或羧酸酯例如6-ACA、另一氨基酸、5-FVA、己二酸/己二酸酯、琥珀酸/琥珀酸酯、乙酸/乙酸酯时，这些术语旨在包括质子化的羧酸(游离酸)、相应的羧酸酯(其共轭碱)及其盐。在本文中提到氨基酸例如6-ACA时，所述术语旨在包括两性离子形式(其中氨基被质子化且羧酸酯基是去质子化的形式)的氨基酸，其中氨基被质子化且羧基为中性形式的氨基酸，和其中氨基为中性形式且羧酸酯基是去质子化形式的氨基酸，以及它们的盐。
提到羧酸的酯或硫代酯例如己二酸酯或硫代酯、乙酸酯或硫代酯、琥珀酸酯或硫代酯时，这些术语旨在包括任何活化基团，尤其是任何生物活化基团，包括辅酶A(也称作CoA)、可与酰基或肽基运载体蛋白质(分别为ACP或PCP)结合的磷酸泛酰巯基乙胺、N-乙酰基-巯乙胺、甲基-硫代-羟乙酸酯、甲基-巯基-丙酸酯、乙基-巯基-丙酸酯、甲基-巯基-丁酸酯、甲基-巯基-丁酸酯、巯基丙酸酯和提供相同或相似功能的其它酯或硫代酯的组。在使用活细胞作为生物催化剂的情况下，酯或硫代酯(尤其是CoA)可通过使用的生物催化剂生产，或来自于也能够生产催化所述反应的合适酶的生物。CoA-连接酶和CoA-转移酶已在许多生物中被鉴定，并且可提供想要的被活化的酯或硫代酯。
由琥珀酸酯或硫代酯制备己二酸酯或硫代酯可尤其包括以下反应步骤(括号中的编号也对应于图1)：
(1)提供琥珀酸酯或硫代酯并使所述酯或硫代酯与乙酸酯或硫代酯反应，从而形成3-氧代己二酸酯或硫代酯；
(2)氢化所述3-氧代己二酸酯或硫代酯的3-氧代基团，从而形成3-羟基己二酸酯或硫代酯；
(3)使所述3-羟基己二酸酯或硫代酯脱水，从而形成2,3-脱氢己二酸酯或硫代酯；和
(4)氢化2,3-脱氢己二酸酯或硫代酯的C-C双键，从而形成己二酸酯或硫代酯。
本发明还涉及用于制备适用于己二酸制备方法中的中间产物化合物的方法，包括在存在催化这类反应步骤的生物催化剂的存在下，进行一个或多个所述反应步骤1-4。
在一个实施方案中，己二酸酯或硫代酯被转化成5-FVA(5)。
如果需要的话，己二酸酯或硫代酯可以被转化成己二酸。这可以通过水解酯键或硫代酯键(7)从而形成己二酸或者通过转移反应来实现，所述转移反应中“醇”或“硫醇”部分(如CoA)被从己二酸酯或硫代酯转移至与己二酸不同的酸，从而形成己二酸和与己二酸不同的酸的(硫代)酯(7)。如果使用琥珀酸或乙酸作为不同的酸，则所述反应可以是有利的，因为所述醇或硫醇部分如CoA可以再循环。例如己二酰-CoA+琥珀酸或乙酸酯可以被转化(通常在存在CoA转移酶时)形成琥珀酰-CoA或乙酰-CoA+己二酸。随后琥珀酰-CoA或乙酰-CoA可以被用作本发明方法中的起始化合物。
己二酸(或其酯或硫代酯)可例如被转化为5-FVA(8)。
在一个实施方案中，在本发明方法中获得的5-FVA被转化成6-ACA(6)。随后6-ACA可以被转化成己内酰胺，例如以本领域本身已知的方式。
在又一个实施方案中，在根据本本发明的方法中获得的己二酸或己内酰胺被用于制备聚酰胺。
除非另有说明，本文中使用的术语“一个”(“a”或“an”)被定义为“至少一个”。
提到单数名词(例如化合物、添加剂等等)时，旨在包括复数在内。
提到存在若干异构体(例如顺式和反式异构体、R和S对映异构体)的化合物时，化合物原则上包括可在本发明具体方法中使用的所述化合物的所有对映异构体、非对映异构体和顺式/反式异构体。
关于括号中的酶种类(EC)提到酶时，所述酶种类是以Nomenclature Committeeof the International Union of Biochemistry and Molecular Biology(NC-IUBMB)提供的Enzyme Nomenclature为基础，将酶归类或可以归类在其中的种类，所述命名法可见http://www.chem.qmul.ac.uk/iubmb/enzyme/。(尚)未归类但是可以同样被归类在特定种类中的其它合适的酶旨在包括在内。
除非另有说明，本文关于登记号提到蛋白质时，所述登记号尤其被用于表示具有在2008年3月11日在Uniprot中找到的序列的蛋白质。
术语“同源物”在本文中尤其用于具有至少30％、优选地至少40％、更优选地至少60％、更优选地至少65％、更优选地至少70％、更优选地至少75％、更优选地至少80％、尤其是至少85％、更尤其是至少90％、至少91％、92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％或至少99％的序列同一性的多核苷酸或多肽。术语同源物也旨在包括由于遗传密码子的简并性而与另一核酸序列不同并且编码相同多肽序列的核酸序列(多核苷酸序列)。
序列同一性或相似性在本文中被定义为两条或更多多肽序列或两条或更多核酸序列之间的相互关系，通过比较所述序列来测定。通常，序列同一性或相似性在序列全长上比较，但是也可以仅在彼此对齐的序列的一部分上比较。在本领域中，“同一性”或“相似性”也表示多肽序列或核酸序列之间之间的序列相关性程度，根据情况由这类序列之间的匹配来确定。测定同一性或相似性的优选方法被设计为在测试的序列之间给出最大匹配。在本发明的上下文中，测定两条序列之间同一性和相似性的一种优选的计算机程序方法包括BLASTP和BLASTN(Altschul，S.F.et al.，J.Mol.Biol.1990，215，403-410)，公众可以从NCBI和其它来源(BLAST Manual，Altschul，S.，et al.，NCBI NLM NIH Bethesda，MD20894)获得。使用BLASTP进行多肽序列比较的优选参数为缺口开放10.0，缺口延伸0.5，Blosum 62矩阵。使用BLASTN进行核酸序列比较的优选参数为缺口开放10.0，缺口延伸0.5，DNA全矩阵(DNA同一性矩阵)。
在本发明的一种方法中，使用生物催化剂，即所述方法中至少一个反应步骤由生物材料或来自生物来源的部分(例如来自生物来源的生物或生物分子)催化。生物催化剂可尤其包含一种或多种酶。生物催化剂可以以任何形式使用。在一个实施方案中，使用从天然环境中分离(从生产它们的生物中分离)的一种或多种酶，例如作为溶液、乳液、分散液、冻干细胞(悬浮液)、裂解物、或固定在支持物上。考虑到调节反应条件时使得反应平衡向想要的一侧偏移的提高的灵活性，从酶的来源生物中分离的酶的用途尤其是有用的。
在一个实施方案中，一种或多种酶形成活生物的一部分(如活的完整细胞)。酶可在细胞内发挥催化功能。酶还可能被分泌进所述细胞存在的培养基中。
活细胞可以是生长中的细胞、静止或休眠(dormant)细胞(例如孢子)或稳定期细胞。还可能使用酶形成通透化(即使其对酶的底物或一种或多种酶的底物前体通透)的细胞的部分。
本发明方法中使用的生物催化剂原则上可以是任何生物，获得得自或来自任何生物。生物可以是真核生物、细菌或古细菌(archea)。具体地，所述生物可选自动物(包括人)、植物、细菌、古细菌、酵母和真菌。
合适的细菌可尤其选自下组：Absidia、Achromobacter、Acinetobacter、Agrobacterium、Aeromonas、Alcaligenes、Arthrobacter、Arzoarcus、Azomonas、Azospirillum、Azotobacter、Bacillus、Beijerinckia、Bradyrhizobium、Burkholderia、Byssochlamys、Citrobacter、Clostridium、Comamonas、Corynebacterium、Deinococcus、Escherichia、Enterobacter、Flavobacterium、Fusobacterium、Gossypium、Klebsiella、Lactobacillus、Listeria、Megasphaera、Micrococcus、Mycobacterium、Norcadia、Porphyromonas、propionibacterium、Pseudomonas、Ralstonia、Rhizobium、Rhodopseudomonas、Rhodospirillum、Rodococcus、Roseburia、Shewanella、Streptomycetes、Xanthomonas、Xylella、Yersinia、Treponema、Vibrio、Streptococcus、Lactococcus、Zymomonas、Staphylococcus、Salmonella、Brucella、Microscilla。
合适的真核细菌可尤其选自下组：真菌；后生动物(metazoan)；Viridiplantae(尤其是Arabidopsis和Chlamydomonadales)；Diplomonads(尤其是Giardiinae)；Entamoebidae(尤其是Entaboeba)；Euglenozoa(尤其是Euglena)；Pelobiontida(尤其是Mastigamoeba)；和Alveolata(尤其是Cryptosporidium)。
合适的真菌尤其包括选自下组的真菌和酵母：Rhizopus、Neurospora、Penicillium、Aspergillus、Piromyces、Trichosporon、Candida、Hansenula、Kluyveromyces、Saccharomyces、Rhodotorula、Schizosaccharomyces、Yarrowia(如Yarrowia lypolytica)。
合适的后生动物尤其包括下述后生动物，所述后生动物选自哺乳动物(包括人)组，更尤其选自Leporidae、Muridae、Suidae、Bovidae、hominidae的组。生物催化剂可源自后生动物的任何部分，例如肝、胰、脑、肾、心或其它器官。合适的后生动物也可尤其包括Caenorhabditis和Drosophila。
描述本发明方法的特定反应步骤时，下文描述了尤其可针对特定反应步骤提供合适生物催化剂的生物。
本领域技术人员应当明白，在根据本发明的方法中可以利用具有合适活性的天然存在的生物催化剂(野生型)或天然存在的生物催化剂的突变体。可以通过本领域技术人员已知的生物学技术，例如分子进化或合理设计(rational design)改进天然存在的生物催化剂的特性。可例如通过使用本领域技术人员已知的诱变技术(随机诱变、定点诱变、定向进化、基因重组等)修饰下述生物的编码DNA来制造野生型生物催化剂的突变体，所述生物能够发挥生物催化剂的作用或者能够生产生物催化剂部分(如酶)。具体地，可以修饰DNA，使其编码与野生型酶差异至少一个氨基酸的酶，使其编码与野生型相比包含一个或多个氨基酸取代、缺失和/或插入的酶，或者使得突变体组合两个或更多亲本酶的序列，或者影响合适的(宿主)细胞中藉此被修饰的DNA的表达。后者可以通过本领域技术人员已知的方法如密码子优化或密码子对优化来实现，例如基于WO 2008/000632中所述方法。
突变体生物催化剂可具有经改进的特性，例如关于一个或多个以下方面：底物选择性、活性、稳定性、溶剂耐受性、pH谱、温度谱、底物谱、对抑制的敏感性、辅因子利用和底物亲和力。可以通过应用例如合适的高通量筛选或选择方法，基于本领域技术人员已知的这类选择方法，来鉴定具有改进的特性的突变体。
可以鉴定作用于烷基或烷基酯或硫代酯的酶的底物特异性。可以利用分子进化产生多样性，随后筛选想要的突变体和/或进行底物结合袋的合理改造。修饰本发明方法中使用的酶底物特异性的技术可基于本领域所述技术。例如，使用结构和序列信息设计特定突变的合理改造已被用于修饰来自红霉素聚酮合酶的酰基转移酶结构域4的底物特异性，使其接受替代性的酰基供体。已经显示修饰提出的底物结合位点得到经修饰的结合袋，所述结合袋能够适应替代性的底物，得到不同的产物比例(Reeves，C.D.；Murli，S.；Ashley，G.W.；Piagentini，M.；Hutchinson，C.R.；McDaniel，R.Biochemistry 2001，40(51)，15464-15470)。合理设计和分子进化途径二者均已被用于改变生物催化剂BM3的底物特异性得到大量下述突变体，所述突变体能够氧化大量多种不同烯烃、环烯烃、芳烃和杂芳烃来代替重链脂肪酸(例如肉豆蔻酸)的天然底物，或除所述天然底物以外还能氧化大量多种不同烯烃、环烯烃、芳烃和杂芳烃(Peters，M.W.；Meinhold，P.；Glieder，A.；Arnold，F.H.Journalof the American Chemical Society 2003，125(44)，13442-13450；Appel，D.；Lutz-Wahl，S.；Fischer，P.；Schwaneberg，U.；Schmid，R.D.Journal of Biotechnology 2001，88(2)，167-171和其中的参考文献)。
涉及来自具体来源的生物催化剂(尤其是酶)、来自第一生物但是实际上在(经遗传修饰的)第二生物中生产的重组生物催化剂(尤其是酶)时，特定地旨在包括来自所述第一生物的生物催化剂(尤其是酶)。
3-氧代己二酸酯(酯或硫代酯)的制备(“反应1”)
在本发明的一个实施方案中，由琥珀酸酯和乙酸酯制备3-氧代己二酸酯(酯或硫代酯)，所述琥珀酸酯和/或乙酸酯促进反应，所述琥珀酸酯和/或乙酸酯通常被提供以活化基团，尤其是为了得到酯或硫代酯。
3-氧代己二酸酯(酯或硫代酯)可以生物催化或化学得到，尤其是通过“Claisen缩合”得到，其中乙酸酯或硫代酯和琥珀酸酯或硫代酯被偶联。
在本发明的一个优选的实施方案中，所述制备包括在存在能够催化酰基转移的生物催化剂下的生物催化反应。因此具有这类催化活性的酶可以被称作酰基转移酶。
在一个优选的方法中，酰基转移发生在两个酰基硫代酯或酰基酯之间。优选的酰基硫代酯是乙酰-CoA和琥珀酰-CoA。优选地，所述生物催化剂对所述酰基硫代酯是选择性的。
生物催化剂可尤其包含能够进行酰基转移的酶，所述酶选自酰基转移酶(E.C.2.3.1)的组，优选地选自乙酰-CoA:乙酰-CoA C-乙酰转移酶(EC 2.3.1.9)、酰基-CoA:乙酰-CoA C-酰基转移酶(EC 2.3.1.16)和琥珀酰-CoA:乙酰-CoA C-琥珀酰转移酶(EC2.3.1.174，也已知为β-酮己二酰-CoA硫解酶)的组。酰基转移酶活性例如在KEGG(KyotoEncyclopedia of Genes and Genomes)数据库中在丁酸酯代谢、聚酮生物合成、脂肪酸生物合成、β-氧化中被描述。
酰基转移酶可尤其是选自古细菌、细菌和真核生物组的生物的酰基转移酶。
具体地，酶可源自能够降解下述有机化合物的微生物，所述有机化合物包含芳香环或脂肪环结构，尤其是5、6或7元环结构。所述有机化合物可任选地在在环中包含一个或多个杂原子，或者作为取代基或取代基的一部分。例如，有机部分可以是芳香族化合物，尤其是包含六元环的芳香族化合物。所述芳香族化合物尤其可选自苯乙酸酯、苯甲酸酯、儿茶酚、原儿茶酸酯(protocatechuate)和龙胆酸酯的组。所述有机化合物可以是脂环族化合物，尤其是环状醇如环己醇，环状酮如环己酮，或环烷如环己烷。有机化合物可以是内酰胺，如己内酰胺。在一个实施方案中，酶来自于能够降解二羧酸(通常为C6–C10)、尤其是直链饱和二羧酸如己二酸的生物。
在又一个实施方案中，源自下述生物、例如源自Streptomycetes(尤其是Streptomyces rimosus)、源自Actinomycres、源自其它Actinobacteria或其它次级代谢产物生产者的酶是优选的，所述生物能够合成3-酮-己二酸酯，例如作为次级代谢产物(例如malonomycin)的部分。
用于提供能够催化3-氧代己二酸酯(酯或硫代酯)制备的生物催化剂的优选的微生物还包括Acinetobacter(尤其是Acinetobacter sp.菌株ADP1和A.calcoaceticus)、Agrobacterium(尤其是A.tumefaciens)、Alicaligenes(尤其是Alicaligenes菌株D2和A.eutrophus)、Arthrobacter、Arzoarcus(尤其是A.evansii)、Azomonas、Azotobacter、Bacillus(尤其是B.halodurans)、Beijerinckia、Bradyrhizobium、Burkholderia、Clostridia(尤其是C.kluyveri、C.acetobutylicum、C.beijerinckii)、Comamonas、Corynebacterium(尤其是C.glutamicum和C.aurantiacum)、E.coli、Enterobacter、Flavobacterium、Megasphera(尤其是M.elsdenii)、Norcadia、Pseudomonas(尤其是P.putida、P.aeruginosa和Pseudomonas sp.菌株B13)、Ralstonia(尤其是R.eutropha)、Rhizobium、Rhodopseudomonas(尤其是R.palustris)、Rodococcus(尤其是R.erythropolis、R.opacus和Rodococcus sp菌株RHA1)、Aspergillus(尤其是A.niger)、Euglenozoa(尤其是Euglena gracilis)、Neurospora(尤其是N.crassa)、Penicillium(尤其是P.chrysogenum)、Rhodotorula、Saccharomyces、Trichosporon(尤其是T.cutaneum)。
在一个特定的实施方案中，生物催化剂包含下述酶，所述酶包含SEQUENCE ID 1-13中任一中鉴定的氨基酸序列或其同源物。
3-羟基己二酸酯(酯或硫代酯)的制备(“反应2”)
在一个实施方案中，由3-氧代己二酸酯(酯或硫代酯)制备3-羟基己二酸酯(酯或硫代酯)。通常，如上文所述对3-氧代己二酸酯提供活化基团。
原则上，可以例如通过选择性氢化3-氧代-己二酸酯(酯或硫代酯)，化学制备3-羟基己二酸酯(酯或硫代酯)。
反应可具体地在存在催化该反应步骤的生物催化剂、尤其是能够催化氧代基团还原(尤其是羰基成为羟基)的生物催化剂时进行。
在一个特定的实施方案中，3-氧代己二酸酯作为其与辅酶A的硫代酯存在(在下文中，3-氧代己二酸酯与辅酶A的硫代酯会被称作3-氧代己二酰-CoA)。
在本发明的一个优选的方法中，制备包含在存在能够催化3-氧代酰基(酯或硫代酯)到3-羟基酰基(酯或硫代酯)的还原的生物催化剂时的生物催化反应。
因此，具有这类催化活性的酶可以被称作3-羟基酰基(酯或硫代酯)脱氢酶。具有针对3-羟基酰基CoA-硫代酯的这类催化活性的酶因此可以被称作3-羟基酰基-CoA脱氢酶。优选地，所述3-羟基酰基-CoA脱氢酶对底物3-氧代己二酰-CoA是选择性的。
能够催化3-氧代酰基(酯或硫代酯)到3-羟基酰基(酯或硫代酯)的还原的酶可尤其选自脱氢酶(E.C.1.1.1)的组，优选地选自3-羟基酰基-CoA脱氢酶(EC 1.1.1.35和EC1.1.1.36)、3-羟基丁酰-CoA脱氢酶(EC 1.1.1.157)、3-羟基庚二酰-CoA脱氢酶(EC1.1.1.259)和长链3-羟基酰基-CoA脱氢酶(EC 1.1.1.211)的组。所述酶可使用NADH或NADPH作为辅因子。3-羟基酰基-CoA脱氢酶活性在例如根据KEGG(Kyoto Encyclopedia ofGenes and Genomes)数据库的芳香族化合物降解、脂肪酸代谢、聚酮生物合成、多羟基链烷酸酯代谢以及丁酸酯代谢中被描述。
微生物尤其能够降解上文定义的有机化合物(见“反应1”)，尤其是芳香族化合物、脂环族化合物或二羧酸。
用于提供能够催化3-羟基己二酸酯(酯或硫代酯)制备的生物催化剂的其它优选的生物包括：Acinetobacter(尤其是Acinetobacter sp.菌株ADP1和A.calcoaceticus)、Alicaligenes(尤其是Alicaligenes菌株D2和A.eutrophus)、Arzoarcus(尤其是A.evansii)、Bacillus(尤其是B.halodurans)、Bordetella(尤其是B.pertussis)、Burkholderia(尤其是B.pseudomallei和B.xenovorans)、Corynebacterium(尤其是C.glutamicum、C.aurantiacum和C.efficiens)、Deinococcus(尤其是D.radiodurans)、E.coli、Flavobacterium、Klebsiella(尤其是K.pneumonia)、Pseudomonas(尤其是P.putida和P.fluorescens)、Rhodopseudomonas(尤其是R.palustris)、Rodococcus(尤其是R.erythropolis、R.opacus和Rodococcus sp菌株RHA1)、Aspergillus(尤其是A.niger)、Neurospora(尤其是N.crassa)、Penicillium(尤其是P.chrysogenum)、Saccharomyces(尤其是S.cerevisiae)。
用于提供作用于3-酮己酰-CoA的EC1.1.1.35酶的一种合适生物可以是包括哺乳动物、尤其是选自Bos taurus、Rattus norvegicus、Sus scrofa和Homo sapiens组的哺乳动物的任何生物。
涉及(厌氧)脂肪酸合成、聚酮生物或称或多羟基链烷酸酯代谢的一种合适的生物催化剂可以来自任何生物，尤其是包括以下的微生物：Clostridia(尤其是C.acetobutylicum和C.kluyveri)、Euglenozoa(尤其是Euglena gracilis)、Megasphera(尤其是Megasphera elsdenii)、Ralstonia(尤其是Ralstonia eutropha)和Zoogloea(尤其是Zoogloea ramigera)。
在一个特定的实施方案中，生物催化剂(催化“反应2”)包含下述酶，所述酶包含SEQUENCE ID 15-26、29任一中鉴定的氨基酸序列或其同源物。预期包含根据SEQUENCEID26的氨基酸序列的酶尤其可催化“反应2”以及“反应3”二者。
2,3-脱氢己二酸酯(酯或硫代酯)的制备(“反应3”)
在一个实施方案中，由3-羟基己二酸酯(酯或硫代酯)制备2,3-脱氢己二酸酯(5-羧基-2-戊烯酸酯)(酯或硫代酯)。任选地，如上文所述，所述2,3-脱氢己二酸酯和3-羟基己二酸酯与辅酶、ACP或另一活化基团偶联。
在本发明的一个实施方案中，通过化学转化3-羟基己二酸酯(酯或硫代酯)，例如在存在例如硫酸时在无水环境中脱氢，来制备2,3-脱氢己二酸酯(酯或硫代酯)。
尤其可使用至少一种生物催化剂，从3-羟基己二酸酯制备2,3-脱氢己二酸酯。
一种优选的生物催化剂是能够催化3-羟基酰基酯或硫代酯到其2-烯酰酯或2-烯酰硫代酯的脱水的生物催化剂。
在一个特定的实施方案中，2,3-脱氢己二酸酯作为其与辅酶A的硫代酯存在(在下文中，2,3-脱氢己二酸酯和辅酶A的硫代酯会被称作5-羧基-2-戊烯-CoA)。
在一个特定的实施方案中，生物催化剂催化3-羟基己二酰-CoA脱水成为5-羧基-2-戊烯-CoA。
生物催化反应尤其可以在存在下述生物催化剂时进行，所述生物催化剂能够催化3-羟基酰基(硫代)酯脱水成为2,3-脱氢酰基硫代酯。
因此，具有这类催化活性的酶可以被称作3-羟基酰基(酯或硫代酯)脱水酶。具有针对3-羟基酰基CoA-硫代酯的这类催化活性的酶因此可以被称作3-羟基酰基-CoA脱水酶。优选地，所述3-羟基酰基-CoA脱水酶对底物3-羟基己二酰-CoA是选择性的。
能够催化3-羟基酰基(酯或硫代酯)脱水成为2,3-脱氢酰基(酯或硫代酯)的酶可尤其选自水解酶(E.C.4.2.1)的组，优选地选自烯酰-CoA水合酶(EC 4.2.1.17)、3-羟基丁酰基-CoA脱水酶(EC 4.2.1.55)和长链-烯酰-CoA水合酶(EC 4.2.1.74)的组。3-羟基酰基-CoA脱水酶活性在例如根据KEGG(Kyoto Encyclopedia of Genes and Genomes)数据库的芳香族化合物降解以及脂肪酸代谢、聚酮生物合成或丁酸酯代谢中被描述。
3-羟基酰基(酯或硫代酯)脱水酶可以是下述生物的3-羟基酰基(酯或硫代酯)脱水酶，所述生物选自古细菌、细菌和真核生物的组，例如选自酵母、真菌和哺乳动物的组。
尤其地，如上文鉴定的(见“反应1”)能够降解有机化合物，尤其是芳香族化合物、脂环族化合物或二羧酸的微生物，是催化2,3-脱氢己二酸酯(酯或硫代酯)制备的生物催化剂的优选来源。
能够降解芳香族化合物、脂环族化合物或二羧酸的微生物包括Acinetobacter(尤其是Acinetobacter sp.菌株ADP1和A.calcoaceticus)、Alicaligenes(尤其是Alicaligenes D2)、Aspergillus(尤其是A.niger)、Azoarcus(尤其是A.evansii)、Bacillus(尤其是B.halodurans)、Corynebacterium(尤其是C.glutamicum和C.aurantiacum)、E.coli、Flavobacterium、Neurospora(尤其是N.crassa)、Penicillium(尤其是P.chrysogenum)、Pseudomonas(尤其是P.putida和P.fluorescens)、Rhodopseudomonas(尤其是R.palustris)、Rhodococcus(尤其是Rhodococcus sp菌株RHA1)。
用于提供作用于3-羟基己酰-CoA的EC4.2.1.17酶的一种优选的生物包括选自哺乳动物和微生物组的生物。来自哺乳动物的一种合适的EC4.2.1.17酶可尤其来自于选自Bos taurus、Homo sapiens、Rattus norvegicus、和Sus scrofa组的哺乳动物。来自微生物的一种合适的EC4.2.1.17酶可尤其来自于选自Aeromonas(尤其是A.caviae)、Clostridium(尤其是C.acetobutylicumi)、Gossypium(尤其是G.hirsutum)、Rhodospirillum(尤其是R.rubrumi)和Ralstonia(尤其是Ralstonia eutropha)组的微生物。
能够进行(厌氧)脂肪酸生物合成的微生物也是优选的。这类微生物包括Clostridia(尤其是C.acetobutylicum和C.kluyveri)、Euglenozoa(尤其是Euglenagracilis)、Megasphera(尤其是M.elsdenii)，和Saccharomyces(尤其是S.cerevisiae)。
一种合适的酶可尤其包含根据SEQUENCE ID 14、27、28、30-41、92任一的氨基酸序列或其同源物。
由2,3-脱氢己二酸酯(酯或硫代酯)制备己二酸酯(酯或硫代酯)(“反应4”)
在一个实施方案中，由2,3-脱氢己二酸酯(酯或硫代酯)制备己二酸酯(酯或硫代酯)。可例如通过选择性氢化C2-C3双键由2,3-脱氢己二酸酯(酯或硫代酯)化学制备，或生物催化地制备己二酸酯(酯或硫代酯)。
通常，对2,3-脱氢己二酸酯提供活化基团，如上文所示。
优选地使用至少一种生物催化剂由2,3-脱氢己二酸酯(酯或硫代酯)制备己二酸酯(酯或硫代酯)，所述生物催化剂催化5-羧基-2-戊烯酸酯(酯或硫代酯)的碳碳双键的脱氢。
在本发明的一个优选的方法中，所述制备包含在存在下述生物催化剂时的生物催化反应，所述生物催化剂能够催化顺式或反式2-烯酰(酯或硫代酯)还原成为酰基(酯或硫代酯)。生物催化剂可以使用多种电子供体，例如选自NADH、NADPH、FADH2和还原型铁氧还蛋白的组的电子供体。电子可从电子供体直接转移至生物催化剂，或者被介导，尤其是由所谓的电子转移黄素蛋白(ETF)介导。因此，具有这类催化活性的一种酶被称作2-烯酰(酯或硫代酯)还原酶(ER)。具有针对2烯酰CoA-硫代酯的这类催化活性的酶因此可以被称作2-烯酰-CoA还原酶。优选地，所述2-烯酰-CoA还原酶对底物2,3-脱氢己二酰基-CoA是选择性的。
能够催化2-烯酰(酯或硫代酯)还原的酶可尤其选自氧化还原酶(EC 1.3.1和EC1.3.99)的组，优选地选自烯酰-CoA还原酶EC 1.3.1.8、EC 1.3.1.38和EC 1.3.1.44的组，选自烯酰-[酰基-运载体-蛋白质]还原酶EC 1.3.1.9、EC 1.3.1.10和EC 1.3.1.39的组，和选自丁酰基-CoA脱氢酶(EC 1.3.99.2)、酰基-CoA脱氢酶(1.3.99.3)和长链-酰基-CoA脱氢酶(EC 1.3.99.13)的组。反式-2-烯酰(酯或硫代酯)还原酶活性已例如在根据KEGG数据库的线粒体脂肪酸生物合成、丁酸酯代谢、聚酮合成和脂肪酸代谢中描述。
2-烯酰(酯或硫代酯)还原酶原则上可得自或衍生自任何生物。所述生物尤其可选自细菌、古细菌或真核生物，如来自酵母、真菌、原生生物、植物和动物(包括人)的组。
在一个实施方案中，生物可选自以下细菌：E.coli、Vibrio、Bacillus(尤其是B.subtilis)、Clostridia(尤其是C.kluyveri、C.acetobutylicum、C.beijerinckii andC.perfringens)、Streptomyces(尤其是S.coelicolor and S.avermitilis)、Pseudomonas(尤其是P.putida and P.aeruginosa)、Shewanella、Xanthomonas、Xylella、Yersinia、Treponema(尤其是T.denticola)、Aeromonas(尤其是Aeromonas hydrophila)、Microscilla(尤其是Microscilla marina)、Megasphera(尤其是Megasphera elsdenii)、Deinococcus(尤其是Deinococcus radiourans)、Yarrowia(尤其是Y.lypolytica)和Eubacterium(尤其是E.pyruvativorans)。
在一个实施方案中，2-烯酰(酯或硫代酯)还原酶来自选自Euglenozoa组的生物(尤其是Euglena gracilis)。
在一个实施方案中，2-烯酰(酯或硫代酯)还原酶来自选自Saccharomyces(尤其是S.cerevisiae)、Kluyveromyces(尤其是K.lactis)、Schizosaccharomyces(尤其是S.pombe)、Candida(尤其是C.tropicalis)组的生物。
在一个实施方案中，2-烯酰(酯或硫代酯)还原酶来自选自Aspergillus(尤其是A.niger和A.nidulans)和Penicillium(尤其是P.chrysogenum)组的生物。
在一个实施方案中，2-烯酰(酯或硫代酯)还原酶来自选自Arabidopsis(尤其是A.thaliana)组的生物。
在一个实施方案中，2-烯酰(酯或硫代酯)还原酶来自选自Homo sapiens、Rattusnorvegicus、Bos Taurus、Cavia sp.、Caenorhabditis elegans和Drosophilamelanogaster组的生物。
合适的酶可尤其包含根据SEQUENCE ID 42-67、94、96、98、100、105、107、109、111、113任一的氨基酸序列或其同源物，尤其是根据SEQUENCE ID 60、63、96、100任一的氨基酸序列或其同源物。编码催化“反应4”的合适酶的示范性核苷酸序列由2-烯酰(酯或硫代酯)还原酶93、95、97、99、104、106、108、110和112表示。
在一个有利的实施方案中，除了2-烯酰(酯或硫代酯)还原酶以外还使用ETF，所述ETF可对所述还原酶的活性有益。这类ETF可得自或衍生自从中可获得或衍生如上文定义的还原酶的生物。其尤其可得自或衍生自与使用的还原酶是相同属、更尤其是相同物种的生物。特定的ETF包含由SEQUENCE ID 102、103、115、116所表示的氨基酸序列。SEQUENCE ID101和114代表编码特定ETF的核苷酸序列。通常，这类ETF包含由两个不同基因编码的两个亚基(etfA和etfB)。这些通常被一起用于使ETF蛋白质具有活性。例如可以使用以下的组合：SEQUENCE ID 102与SEQUENCE ID 103或SEQUENCE ID 116与SEQUENCE ID 115。技术人员应当能够选择本领域本身已知的其它合适的ETF组合。
在本发明的一个实施方案中，可以通过本领域已知的方法修饰本身不具有想要的活性或底物特异性的生物催化剂，例如通过合理设计或分子进化产生能够以想要的速率或选择性催化2,3-脱氢己二酸酯(酯或硫代酯)转化成为己二酸酯(酯或硫代酯)的突变体。对链长为6的2-烯酰-CoA衍生物具有活性的生物催化剂，尤其是来自C.kluyveri、Bostaurus、Euglena gracilis、Cavia sp.、S.cerevisiae、C.tropicalis、Homo sapiens和E.pyruvativorans的这类生物催化剂是优选的。
己二酸的制备(“反应7”)
根据本发明，可以使用己二酸酯或硫代酯，通过酯键或硫代酯键的水解制备己二酸。这可以化学完成，例如在存在酸或碱时通过化学水解完成，或者生物催化地完成。
在本发明的一个优选的方法中，制备包含在存在能够催化酰基(硫代)酯水解的生物催化剂时的生物催化反应。
具有这类催化活性的酶因此可以被称作酰基(硫代)酯水解酶。具有针对酰基-CoA硫代酯的这类催化活性的酶因此可以被称作酰基-CoA水解酶。优选地，所述酰基-CoA水解酶对底物己二酰基-CoA是选择性的。
能够催化水解酰基(硫代)酯的酶可尤其选自水解酶(EC 3.1.2)的组，优选地选自酰基-CoA水解酶(EC 3.1.2.20)、乙酰-CoA水解酶(EC 3.1.2.1)、长链脂肪酰基CoA-水解酶(EC 3.1.2.2)、琥珀酰-CoA水解酶(EC 3.1.2.3)和酰基-[酰基-运载体-蛋白质]-水解酶(EC 3.1.2.14)的组。
生物催化剂可包含来自任何生物(包括古细菌、细菌或真核生物)的酶。
生物催化剂尤其可包含下述细菌的酶，所述细菌选自E.coli、Brucella(尤其是Brucella melitensis)、Agrobacterium(尤其是A.tumefaciens)、Xanthomonas、Sinorhizobium(尤其是Sinorhizobium meliloti)、Mesorhizobium(尤其是Mesorhizobiumloti)、Vibrio、Streptomyces(尤其是S.coelicolor和S.avermitilis)、Rhodopseudomonas(尤其是Rhodopseudomonas palustris)、Xylella、Yersinia、Pseudomonas(尤其是P.putida和P.aeruginosa)、Shewanella、Shigella、Salmonella、Corynebacterium、Mycobacterium、Hyphomonas(尤其是Hyphomonas neptunium)和Propionibacterium的组。
合适的生物催化剂可尤其存在于选自Saccharomyces(尤其是Saccharomycescerevisiae)和Kluyveromyces(尤其是K.lactis)组的酵母中。
合适的生物催化剂可尤其存在于选自Aspergillus(尤其是A.niger、A.fumigatus和A.nidulans)和Penicillium(尤其是P.chrysogenum)组的真菌中。
在又一个实施方案中，所述生物选自Arabidopsis(尤其是A.thaliana)、Muridae(尤其是Rattus norvegicus、Mus musculus)、Bovidae(尤其是Bos taurus、Ovis aries)、Homo sapiens和Caenorhabditis(尤其是Caenorhabditis elegans)的组。
在本发明的一个实施方案中，可以通过本领域已知的方法修饰本身不具有想要的活性或底物特异性的生物催化剂，例如通过合理设计或分子进化产生能够将己二酸酯或硫代酯有效转化成己二酸酯的突变体。对C4-C8酸的酰基-CoA衍生物(优选地包括二羧酸)具有初始活性的生物催化剂是优选的。例如，可以基于来自Mus musculus的酰基-CoA-硫酯酶(例如Seq ID 73中所给出的)产生突变体。
在本发明的一个特定的实施方案中，制备包含在存在下述生物催化剂时的生物催化反应，所述生物催化剂能够催化活化基团、尤其是酯或硫代酯、最尤其是CoA的转移。
具有这类催化活性的酶因此可以被称作CoA转移酶。优选地，所述CoA转移酶对作为CoA-供体底物的二羧酸-CoA是选择性的。更优选地，所述二羧酸-CoA是己二酰-CoA。优选地，所述CoA转移酶对作为CoA-接受底物的乙酸酯是选择性的。
能够催化CoA基团转移的酶可尤其选自CoA转移酶(EC 2.8.3)的组，优选地选自二羧酸-CoA:二羧酸CoA转移酶、己二酸酯:琥珀酰-CoACoA转移酶、3-氧代酸CoA-转移酶(EC2.8.3.5)、3-氧代己二酸酯CoA-转移酶(EC 2.8.3.6)和乙酸酯CoA-转移酶(EC 2.8.3.8)的组。
CoA转移酶原则上可以得自或衍生自任何生物。所述生物可以是细菌、古细菌或真核生物。能够降解二羧酸、尤其是己二酸的生物尤其是优选的。
生物可尤其是选自下组的细菌：Acinetobacter(尤其是Acinetobacter菌株ADP1、A.calcoaceticus)、Clostridium(尤其是C.kluyveri、C.acetobutylicum或C.beijerinckii)、Pseudomonas(尤其是P.putida和P.fluorescens)、Agrobacterium、Alcaligenes、Athrobacter、Azomonas、Azospirillum、Azotobacter、Bacillus、Beijerinckia、Bradyrhizobium、Burkholderia、Comamonas、Corynebacterium、Norcadia、Rhizobium、Rhodotorula、Rodococcus、Trichosporon和Roseburia sp.。
生物可尤其是选自Aspergillus(尤其是A.niger)、Penicillium(尤其是P.chrysogenum)和Neurospora的组的酵母或真菌。
合适的CoA转移酶尤其可得自或衍生自来自Hominidea科的物种，更尤其是来自于Homo sapiens。
反应7的合适酶可尤其包含根据SEQUENCE ID 68-73、85、116、117、119-124任一的氨基酸序列或其同源物。
由硫代酯制备己二酸可尤其由包含下述酰基-CoA水解酶的生物催化剂催化，所述酰基-CoA水解酶包含根据SEQUENCE ID 68-73、117、119任一的氨基酸序列或其同源物。
由硫代酯制备己二酸可尤其由包含下述CoA转移酶的生物催化剂催化，所述CoA转移酶包含根据SEQUENCE ID 85、121、122、123、124、125、126任一的氨基酸序列或其同源物。
CoA转移酶可由单个基因或多于一个基因编码。例如，一些CoA-转移酶包含由两个不同基因编码的两个亚基。这些通常被一起用于使CoA转移酶蛋白质具有活性。例如可以使用以下的组合：SEQUENCE ID 121与SEQUENCE ID 122，或SEQUENCE ID 125与SEQUENCE ID126。
由己二酸酯或己二酸硫代酯制备5-FVA(“反应”5)
在一个实施方案中，由己二酸酯或己二酸硫代酯制备5-甲酰基戊酸酯(5-FVA)。这可以化学或生物催化地完成。己二酸酯可尤其与CoA或另一活化基团偶联，如上文所示。
本发明尤其还提供了在存在能够催化酰基酯或硫代酯还原成为醛的催化剂时，由己二酸酯或己二酸酯硫代酯制备5-FVA的方法，尤其是由己二酰-CoA硫代酯制备5-FVA的方法。
具有这类催化活性的酶因此可以被称作醛脱氢酶。对酰基酯或酰基硫代酯(例如酰基-CoA硫代酯)具有这类催化活性的酶可因子被称作醛脱氢酶(乙酰化)。优选地，包含醛脱氢酶(乙酰化)的生物催化剂对底物己二酸酯或硫代酯是选择性的。
能够催化酰基(硫代)酯还原的酶可尤其选自氧化还原酶(EC1.2.1)的组，优选地选自醛脱氢酶(乙酰化)(EC 1.2.1.10)、脂肪酰基-CoA还原酶(EC 1.2.1.42)、长链脂肪酰基-CoA还原酶(EC 1.2.1.50)、丁醛脱氢酶(EC 1.2.1.57)和琥珀酸酯半醛脱氢酶(乙酰化)的组(见例如Sohling et al.1996.J Bacteriol.178:871-880)。
醛脱氢酶原则上可得自或衍生自任何生物。应当理解也可以通过直接分离编码核酸并随后测定异源宿主中的活性，或者通过宏基因组DNA中发现的序列同一性，从宏基因组来源获得所述酶。所述生物可以是细菌、古细菌或真核生物。所述生物尤其可选自细菌，更尤其选自E.coli、Clostridium(尤其是C.kluyveri、、C.beijerinckii、C.acetobutylicum、C.botylicum、C.tetani、C.perfringens and C.novyi)、Porphyromonasgingivalis、Listeria、Propionibacterium(尤其是P.freudenreichii)、Enterococcus、Fusobacterium、Lactobacillus(尤其是L.lactis)、Bacillus(尤其是B.thuringiensis)、Burkholderia(尤其是B.thailandensis和B.mallei)、Pseudomonas(尤其是P.putida)、Rhodococcus(尤其是R.sp.RHA1)和Salmonella(尤其是S.typhimurium)的组。所述生物也可以选自真核生物、更尤其选自Giardia(尤其是G.lamblia)、Entamoeba(尤其是E.Histolytica)、Mastigamoeba balamuthi、Chlamydomonas reinhardtii、Polytomella、Piromyces、Cryptosporidium和Spironucleus barkhanus的组。
合适的脱氢酶可尤其包含根据SEQUENCE ID 74-81、139-148任一的氨基酸序列或其同源物。
在本发明的一个实施方案中，可以通过本领域已知的方法修饰本身不具有想要的活性或底物特异性的生物催化剂，例如通过合理设计或分子进化产生能够将己二酸酯或硫代酯转化成5-FVA的突变体。对链长4-8的酰基-CoA衍生物具有酰化醛脱氢酶活性的生物催化剂是优选的，包括但不限于生物催化剂如来自C.kluyveri(SEQUENCE ID 74)或P.gingivialis(SEQUENCE ID 75)的琥珀酸酯半醛脱氢酶(乙酰化)和来自C.acetobutylicum(SEQUENCE ID 80，81)或Propionibacterium freudenreichii(SEQUENCE ID 79)的丁醛脱氢酶(乙酰化)。
由己二酸制备5-FVA(“反应8”)
根据本发明，己二酸可以被用于通过还原羧酸基团之一来制备5-FVA。这可通过选择性化学还原(任选地包括一个羧酸基的保护)化学地完成，或生物催化地完成。在本发明的一个优选的实施方案中，制备包含在存在能够催化羧酸还原的生物催化剂时的生物催化反应。生物催化剂可以使用NADH或NADPH作为电子供体。
具有这类催化活性的酶因此可以被称作醛脱氢酶。优选地，所述醛脱氢酶对底物己二酸酯是选择性的。
能够催化羧酸还原的酶可尤其选自氧化还原酶(EC 1.2.1)的组，优选地选自醛脱氢酶(EC 1.2.1.3、EC 1.2.1.4和EC 1.2.1.5)、丙二酸酯-半醛脱氢酶(EC 1.2.1.15)、琥珀酸酯-半醛脱氢酶(EC 1.2.1.16和EC 1.2.1.24)、戊二酸酯半醛脱氢酶(EC 1.2.1.20)、氨基己二酸酯半醛脱氢酶(EC 1.2.1.31)、己二酸酯半醛脱氢酶(EC 1.2.1.63)的组，其也可以被称作6-氧代己酸酯脱氢酶。己二酸酯半醛脱氢酶活性已例如在根据KEGG数据库中的己内酰胺降解通路中被描述。尤其可使用6-氧代己酸酯脱氢酶。6-氧代己酸酯脱氢酶的例子是包含SEQUENCE ID 127、128所示序列或其同源物的酶。
醛脱氢酶原则上可来自或衍生自任何生物。生物可以是原核生物或真核生物。所述生物可尤其选自细菌、古细菌、酵母、真菌、原生生物、植物和动物(包括人)。
在一个实施方案中，所述细菌选自Acinetobacter(尤其是Acinetobactersp.NCIMB9871)、Ralstonia、Bordetella、Burkholderia、Methylobacterium、Xanthobacter、Sinorhizobium、Rhizobium、Nitrobacter、Brucella(尤其是B.melitensis)、Pseudomonas、Agrobacterium(尤其是Agrobacterium tumefaciens)、Bacillus、Listeria、Alcaligenes、Corynebacterium和Flavobacterium的组。
在一个实施方案中，所述生物选自酵母和真菌的组，尤其是选自Aspergillus(尤其是A.niger和A.nidulans)和Penicillium(尤其是P.chrysogenum)的组。
在一个实施方案中，所述生物是植物，尤其是Arabidopsis，更尤其是A.thaliana。
6-ACA的制备(“反应6”)
在本发明的一个实施方案中，5-FVA被用于制备6-ACA。
在一个实施方案中，通过在PtO2上氢化6-oximocaproic acid制备6-ACA，所述6-oximocaproic acid通过5-FVA和羟胺的反应制备(同源的12-氨基正十二烷酸的合成见例如F.O.Ayorinde，E.Y.Nana，P.D.Nicely，A.S.Woods，E.O.Price，C.P.Nwaonicha J.Am.OilChem.Soc.1997，74，531-538)。
可以在氢化催化剂(例如SiO2/Al2O3支持物上的Ni)上通过用氨还原性氨化5-FVA，以高产率制备6-ACA，如EP-A 628 535或DE 4 322 065中针对9-氨基壬酸(9-氨基正壬酸)和12-氨基正十二烷酸(12-氨基月桂酸)所述。
在又一个实施方案中，生物催化地制备6-ACA。在一个优选的方法中，由5-FVA到6-ACA的制备包含在存在下述酶时的酶促反应，所述酶能够在存在氨基供体时催化转氨基反应，所述酶选自氨基转移酶(E.C.2.6.1)的组。
通常，合适的氨基转移酶具有6-ACA 6-氨基转移酶活性，能够催化5-FVA转化成6-ACA。
氨基转移酶可尤其选自来自以下的的氨基转移酶：哺乳动物；Mercurialis，尤其是Mercurialis perennis，更尤其是Mercurialis perennis的嫩芽(shoots)；Asplenium，更尤其是Asplenium unilaterale或Asplenium septentrionale；Ceratonia，更尤其是Ceratonia siliqua；Rhodobacter，尤其是Rhodobacter sphaeroides，Staphylococcus，尤其是Staphylococcus aureus；Vibrio，尤其是Vibrio fluvialis；Pseudomonas，尤其是Pseudomonas aeruginosa；Rhodopseusomonas；Bacillus，尤其是Bacillusweihenstephanensis和Bacillus subtilis；Legionella；Nitrosomas；Neisseria；或酵母，尤其是Saccharomyces cerevisiae。
当酶是哺乳动物的酶时，其尤其可源自哺乳动物肾，来自哺乳动物肝，来自哺乳动物心或来自哺乳动物脑。例如，合适的酶可选自下组：来自哺乳动物肾的β-氨基异丁酸酯:α-酮戊二酸酯氨基转移酶，尤其是来自猪肾的β-氨基异丁酸酯:α-酮戊二酸酯氨基转移酶；来自哺乳动物肝的β-丙氨酸氨基转移酶，尤其是来自兔肝的β-丙氨酸氨基转移酶；来自哺乳动物心的天冬氨酸氨基转移酶；尤其是来自猪心的天冬氨酸氨基转移酶；来自哺乳动物肝的4-氨基-丁酸酯氨基转移酶，尤其是来自猪肝的4-氨基-丁酸酯氨基转移酶；来自哺乳动物脑的4-氨基-丁酸酯氨基转移酶，尤其是来自人、猪或大鼠脑的4-氨基丁酸酯氨基转移酶；来自Neurospora的α-酮己二酸酯-谷氨酸酯氨基转移酶，尤其是来自Neurospora crass的α-酮己二酸酯:谷氨酸酯氨基转移酶；来自E.coli的4-氨基-丁酸酯氨基转移酶，或来自Thermus的α-氨基己二酸酯氨基转移酶，尤其是来自Thermus thermophilus的α-氨基己二酸酯氨基转移酶，和来自Clostridium、尤其是来自Clostridium aminovalericum的5-氨基戊酸酯氨基转移酶。一种合适的2-氨基己二酸酯氨基转移酶可例如由Pyrobaculumislandicum提供。
在一个特定的实施方案中，使用包含根据Sequence ID 82、Sequence ID 83、Sequence ID 84、Sequence ID 134、Sequence ID 136、Sequence 138的氨基酸序列或任何这些序列的同源物的氨基转移酶。Sequence ID 86(野生型)和88(经密码子优化)代表编码Sequence ID 82(＝87)所示酶的序列。Sequence ID 89(野生型)和91(经密码子优化)代表编码SequenceID 83(＝90)所示酶的序列。Sequence ID 133、Sequence ID 135、Sequence137分别代表Sequence ID 134、Sequence ID 136、Sequence 138的编码序列。
氨基供体尤其可选自氨、铵离子、胺和氨基酸的组。合适的胺是伯胺和仲胺。氨基酸可具有D-构型或L-构型。氨基供体的例子是丙氨酸、谷氨酸、异丙胺、2-氨基丁烷、2-氨基庚烷、苯甲胺、1-苯基-1-氨基乙烷、谷氨酰胺、酪氨酸、苯丙氨酸、天冬氨酸、β-氨基异丁酸酯、β-丙氨酸、4-氨基丁酸酯和α-氨基己二酸酯。
在又一个优选的实施方案中，制备6-ACA的方法包含在存在下述酶时的生物催化反应，所述酶能够在存在氨来源时催化还原性氨基化反应，所述酶选自作用于供体CH-NH2基团上的氧化还原酶(EC 1.4)的组，尤其是选自氨基酸脱氢酶(E.C.1.4.1)的组。通常，合适的氨基酸脱氢酶具有催化5-FVA转化成为6-ACA的6-氨基己酸6-脱氢酶活性，或者具有催化AKP转化成为AAP的α-氨基庚二酸酯2-脱氢酶活性。合适的氨基酸脱氢酶尤其选自二氨基庚二酸酯脱氢酶(EC 1.4.1.16)、赖氨酸6-脱氢酶(EC 1.4.1.18)、谷氨酸脱氢酶(EC1.4.1.3；EC 1.4.1.4)和亮氨酸脱氢酶(EC 1.4.1.9)的组。
在一个实施方案中，氨基酸脱氢酶可选自被归类为以下的氨基酸脱氢酶：以NAD或NADP作为受体的谷氨酸脱氢酶(EC 1.4.1.3)、以NADP作为受体(EC 1.4.1.4)的谷氨酸脱氢酶、亮氨酸脱氢酶(EC 1.4.1.9)、二氨基庚二酸酯脱氢酶(EC 1.4.1.16)和赖氨酸6-脱氢酶(EC 1.4.1.18)。
氨基酸脱氢酶可尤其源自选自下组的生物：Corynebacterium，尤其是Corynebacterium glutamicum；Proteus，尤其是Proteus vulgaris；Agrobacterium，尤其是Agrobacterium tumefaciens；Geobacillus，尤其是Geobacillus stearothermophilus；Acinetobacter，尤其是Acinetobacter sp.ADP1；Ralstonia，尤其是Ralstoniasolanacearum；Salmonella，尤其是Salmonella typhimurium；Saccharomyces，尤其是Saccharomyces cerevisiae；Brevibacterium，尤其是Brevibacterium flavum；和Bacillus，尤其是Bacillus sphaericus、Bacillus cereus或Bacillus subtilis。例如，合适的氨基酸脱氢酶可选自来自Bacillus，尤其是Bacillus sphaericus的二氨基庚二酸酯脱氢酶；来自Brevibacterium sp.的二氨基庚二酸酯脱氢酶；来自Corynebacterium的二氨基庚二酸酯脱氢酶，尤其是来自Corynebacterium glutamicum的二氨基庚二酸酯脱氢酶；来自Proteus的二氨基庚二酸酯脱氢酶，尤其是来自Proteus vulgaris的二氨基庚二酸酯脱氢酶；来自Agrobacterium、尤其是Agrobacterium tumefaciens的赖氨酸6-脱氢酶，来自Geobacillus、尤其是来自Geobacillus stearothermophilus的赖氨酸6-脱氢酶；来自Acinetobacter的以NADH或NADPH作为辅因子发挥作用的谷氨酸酯脱氢酶(EC 1.4.1.3)，尤其是来自Acinetobacter sp.ADP1的谷氨酸酯脱氢酶；来自Ralstonia的谷氨酸酯脱氢酶(EC 1.4.1.3)，尤其是来自Ralstonia solanacearum的谷氨酸酯脱氢酶；来自Salmonella的以NADPH作为辅因子发挥作用的，尤其是来自Salmonella typhimurium的谷氨酸酯脱氢酶；来自Saccharomyces的谷氨酸酯脱氢酶(EC 1.4.1.4)，尤其是来自Saccharomycescerevisiae的谷氨酸酯脱氢酶；来自Brevibacterium的谷氨酸酯脱氢酶，尤其是来自Brevibacterium flavum的谷氨酸酯脱氢酶；和来自Bacillus的谷氨酸酯脱氢酶，尤其是来自Bacillus cereus或Bacillus subtilis的谷氨酸酯脱氢酶。
在一个实施方案中，在本发明方法中制备的6-ACA被用于制备己内酰胺。这类方法包括环化6氨基己酸，任选地在存在生物催化剂时进行。
本发明上下文中任何生物催化步骤的反应条件可根据生物催化剂、尤其是酶的已知条件，本文公开的信息和任选地一些常规实验来选择。
原则上，使用的反应培养基的pH可以在宽泛的界限内选择，制药生物催化剂在所述pH条件下有活性即可。可以使用碱性、中性或酸性条件，取决于生物催化剂和其它因素。当所述方法包括使用微生物(例如用于表达催化本发明方法的酶)时，选择pH使得所述微生物能够发挥其预期的一种或多种功能。在25℃下基本水性体系的情况下，尤其可在低于中性pH四个pH单位和高于中性pH两个pH单位的范围内选择pH，即在pH 3和pH9之间选择。如果水是唯一的溶剂或主要的溶剂(以总液体为基础>50wt.％，尤其是>90wt.％)，则系统被认为是水性的，其中例如小量(以总液体为基础<50wt.％，尤其是<10wt.％)的醇或另一种溶剂可以下述浓度溶解(例如作为碳源)，所述浓度使得可以存在的微生物保持活性。尤其是在使用酵母和/或真菌的情况下，可优选酸性条件，尤其是25℃下基于基本水性体系的pH可以在pH 3到pH 8的范围内。需要时可以使用酸和/或碱调节或者用合适的酸和碱的组合缓冲pH。
原则上，孵育条件可以在在广阔的界限内选择，只要生物催化剂显示足够的活性和/或生长即可。这包括需氧、微需氧、限氧和厌氧的条件。
厌氧条件在本文中被定义为下述条件：无任何氧，或其中生物催化剂(尤其是微生物)基本不消耗氧并且通常对应于少于5mmol/l.h的氧消耗，尤其是小于2.5mmol/l.h的氧消耗，或小于1mmol/l.h的氧消耗。
需氧条件是下述条件，其中对不受限制的生长而言足够水平的氧溶于培养基中，能够支持至少10mmol/l.h、更优选地多于20mmol/l.h、进一步更优选地多于50mmol/l.h、最优选地多于100mmol/l.h的氧消耗速率。
限氧条件被定义为下述条件：其中氧消耗由从气体转移至液体的氧限制。限氧条件的下限由厌氧条件的上限决定，即至少1mmol/l.h，尤其是至少2.5mmol/l.h，或至少5mmol/l.h。限氧条件的上限由需氧条件的下限决定，即少于100mmol/l.h、少于50mmol/l.h、少于20mmol/l.h或少于10mmol/l.h。
条件是需氧、厌氧还是限氧取决于所述方法进行的条件，尤其是进入气流的量和组成、使用的设备的实际混合/质量转移特性、使用的微生物的类型和微生物密度。
原则上，使用的温度不是关键性的，只要生物催化剂(尤其是酶)显示大量活性即可。通常，温度可以是至少0℃，尤其是至少15℃，更尤其是至少20℃。想要的最大温度取决于生物催化剂。通常这类最大温度是本领域已知的，例如在可商业获得的生物催化剂的情况下在产品数据表中指出，或者可以基于公知常识和本文公开的信息常规地测定。温度通常是90℃或更少，优选地是70℃或更少，尤其是50℃或更少，更尤其是40℃或更少。
另外，溶剂、额外的试剂和其它助剂例如辅因子(例如FAD/FADH和/或NAD/NADH辅因子)可基于已知的反应原则选择，来完成或加速特定的反应和/或可以采取手段使平衡向想要的一侧偏移。具体地，如果生物催化反应在宿主生物外进行，则可以高浓度(例如大于50％，或大于90wt.％)使用包含有机溶剂的反应培养基，使得使用的酶在这样的培养基中保持足够的活性。
在本发明方法中使用的琥珀酸酯(酯或硫代酯)和乙酸酯(酯或硫代酯)原则上可以通过任何方式获得。
琥珀酸酯例如作为柠檬酸循环(Krebs循环)的中间产物或细胞代谢的终产物而天然形成。因此，其可通过使用合适的生物催化剂得自可更新的碳源。生物催化剂(尤其是微生物)可被用于从合适的碳源生产琥珀酸酯。微生物可以是原核生物或真核生物。微生物可以是重组的或野生型。
在重组微生物中，代谢可以被改变，以提高琥珀酸酯在合适碳源上的产率和生产力。用于提高琥珀酸生产的方法已在Song and Lee，Enzyme and Microbial Technology，2006，39:352-361中针对原核生物描述。琥珀酸酯也可以在真核生物中生产。另外和替代性地，可以应用适应性进化，如美国申请2007/111294中所述。
琥珀酸酯或硫代酯可以任何方式得自琥珀酸酯。琥珀酸酯或硫代酯可尤其通过使用生物催化剂得自琥珀酸酯。琥珀酰-CoA可尤其通过使用包含下述酶的生物催化剂而得自琥珀酸酯，所述酶选自酸性硫醇连接酶(EC 6.2.1)的组，优选地选自琥珀酰-CoA合酶(EC6.2.1.4和EC 6.2.1.5)的组。另外或替代性地，琥珀酰-CoA可通过使用包含下述酶的生物催化剂而得自琥珀酸酯，所述酶选自如针对反应7所述CoA转移酶(EC 2.8.3)的组。
琥珀酸酯或硫代酯也可以任何方式得自除琥珀酸酯之外的其它分子。具体地，琥珀酰-CoA可使用包含2-氧代谷氨酸酯脱氢酶复合物的生物催化剂，得自2-氧代谷氨酸酯。2-氧代谷氨酸酯脱氢酶复合物是本领域技术人员已知的参与TCA循环的多酶复合物。另外或替代性地，琥珀酰-CoA可使用包含2-氧代谷氨酸酯:铁氧还蛋白氧化还原酶(EC1.2.7.3)得自2-氧代谷氨酸酯。
乙酸酯是细胞代谢中的一种天然中间产物或终产物。因此，其可通过使用合适的生物催化剂得自可更新的碳源。生物催化剂(尤其是微生物)可被用于从合适的碳源生产琥珀酸酯。微生物可以是重组的或野生型。
乙酸酯或硫代酯可以任何方式得自乙酸酯。具体地，乙酰-CoA可通过使用包含下述酶的生物催化剂得自乙酸酯，所述酶选自酸性硫醇连接酶(EC 6.2.1)的组，优选地选自乙酰-CoA合酶(EC 6.2.1.1和EC 6.2.1.13)的组。另外或替代性地，乙酰-CoA可使用包含下述酶的生物催化剂而得自乙酸酯，所述酶选自如针对反应7所述CoA转移酶(EC 2.8.3)的组。
乙酸酯或硫代酯也可以任何方式得自除乙酸酯之外的其它分子。具体地，乙酰-CoA可使用包含下述酶的生物催化剂得自丙酮酸酯，所述酶选自丙酮酸酯脱氢酶复合物、丙酮酸酯脱氢酶(NADP+)(EC 1.2.1.51)、丙酮酸酯甲酸酯裂合酶(EC 2.3.1.54)，或有效地将丙酮酸酯转化成乙酰-CoA的生物催化剂或酶。丙酮酸酯脱氢酶复合物是本领域技术人员已知的将丙酮酸酯转化成乙酰-CoA的多酶复合物。
乙酰-CoA也可以使用包含以下酶的生物催化剂得自乙醛，所述酶选自氧化还原酶(EC 1.2.1)的组，优选地选自醛脱氢酶(乙酰化)、脂肪酰基-CoA还原酶(EC 1.2.1.42)、丁醛脱氢酶(EC 1.2.1.57)和琥珀酸酯半醛脱氢酶(乙酰化)的组(如Sohling et al.1996.JBacteriol.178:871-880中所述)。
当生物催化剂是真核生物时，可以通过过表达编码下述酶的同源和/或异源基因，来提高乙酰-CoA的供应，优选地提高宿主细胞胞质区室中乙酰-CoA的供应，所述酶催化前体分子成为乙酰-CoA的转化。前体分子可例如是乙酸酯，如Shiba et al.，MetabolicEngineering，2007，9:160-8所述。
在本发明的一种有利方法中，尤其是制备6-ACA、己二酸或6-ACA或己二酸的中间产物化合物的一种方法中，利用6-ACA、己二酸或其中间产物的底物的全细胞生物转化，包括使用其中生产一种或多种酶催化任何上述反应的微生物和所述微生物的碳源。
碳源可尤其含有至少一种选自下组的化合物：一元醇、多元醇、羧酸、一氧化碳、脂肪酸、甘油酯，包括任何所述化合物的混合物。合适的一元醇包括甲醇和乙醇。合适的多元醇包括甘油和碳水化合物。合适的脂肪酸或甘油三酯可尤其以食用油的形式提供，优选地为植物来源。
尤其可以使用碳水化合物，因为通常碳水化合物可从生物可更新来源如农产品(例如农业废料)中大量获得。优选地，使用选自下组的碳水化合物：葡萄糖、果糖、蔗糖、乳糖、蔗二糖、淀粉、纤维素和半纤维素。尤其优选的是葡萄糖、包含葡萄糖的寡糖和包含葡萄糖的多糖。
在本发明的一个特定方法中，所述方法是发酵方法。这类方法可尤其包括将包含生物催化剂的细胞(任选地为本文所述的宿主细胞)与可发酵的碳源接触，其中所述碳源含有任何要被转化成待制备化合物的所述化合物，或其中所述细胞将要被转化成要用碳源制备的化合物的化合物。
可以使用本领域本身已知的分子生物学技术，构建包含用于在本发明方法中催化反应步骤的一种或多种酶的细胞。例如，如果要在异源体系中生产一种或多种生物催化剂，则这类技术可以被用于提供下述载体，所述载体包含一个或多个编码一种或多种所述生物催化剂的基因。包含一个或多个这类基因的载体可包含一个或多个调节元件，例如一个或多个启动子，所述启动子可与编码生物催化剂的基因可操作地连接。
在本文中使用时，术语“可操作地连接”是指功能性相互关系中多核苷酸元件(或编码序列或核酸序列)的一种连接。当核酸序列被置于与另一核酸序列的功能性相互关系之中时，所述核酸序列是“可操作地连接”的。例如，如果启动子或增强子影响编码序列的转录，则所述启动子或增强子与所述编码序列可操作地连接。
在本文中使用时，术语“启动子”是指一种核酸片段，其功能是控制一个或多个基因的转录，根据转录的方向位于基因的转录起点上游，并且结构上由DNA-依赖性RNA聚合酶、转录起点和任何其它DNA序列的存在识别，所述任何其它DNA序列包括但不限于转录因子结合位点、阻抑因子和激活因子蛋白结合位点和本领域技术人员已知的直接或间接地作用以调节来自启动子的转录量的任何其它核苷酸序列。“组成型”启动子是在大部分环境和发育条件下有活性的启动子。“诱导型”启动子是在环境或发育调节下有活性的启动子。当用于指出给定的(重组的)核酸或多肽分子与给定的宿主生物或宿主细胞之间的相互关系时，术语“同源的”应当被理解为表示该核酸或多肽分子天然地由相同物种(优选地相同变种或菌株)的宿主细胞或生物生产。
可用于实现编码本发明方法中使用的酶(如上文所述)的核苷酸序列表达的启动子对编码要表达的酶的核苷酸序列而言可以是天然的，或者可以对与之可操作地连接的核苷酸序列而言是异源的。优选地，启动子是同源的，即对宿主细胞而言是内源的。
如果使用(对编码感兴趣的酶的核苷酸序列而言)异源启动子，则所述异源启动子优选地能够生产比编码序列的天然启动子更高稳态水平的包含所述编码序列的转录本(或者单位时间能够生产更多转录本分子，即mRNA分子)。本发明上下文中合适的启动子包括本领域技术人员公知的组成型和诱导型启动子以及经改造的启动子。
“强组成型启动子”是与天然宿主细胞相比引起mRNA以高频率起始的启动子。革兰氏阳性微生物中这类强组成型启动子的例子包括SP01-26、SP01-15、veg、pyc(丙酮酸羧化酶启动子)和amyE。
革兰氏阳性微生物中诱导型启动子的例子包括IPTG诱导型Pspac启动子，木糖诱导型PxylA启动子。
革兰氏阴性微生物中组成型和诱导型启动子的例子包括tac、tet、trp-tet、lpp、lac、lpp-lac、lacIq、T7、T5、T3、gal、trc、ara(PBAD)、SP6、λ-PR和λ-PL。
在关于核酸(DNA或RNA)或蛋白质的方面，使用术语“异源的”表示下述核酸或蛋白质，其不作为其存在的生物、细胞、基因组或DNA或RNA序列的部分天然存在，或其存在于与其天然存在的细胞或位置基因组或DNA或RNA序列中的位点不同的地方。异源核酸或蛋白质对其被引入的细胞而言不是内源的，但是得自另一细胞或被合成或重组地生产。一般地(尽管并非必须)，这类核酸编码下述细胞通常不生产的蛋白质，所述DNA在所述细胞中被转录或表达。类似地，外源RNA编码下述蛋白质，所述蛋白质在存在所述外源RNA的细胞中通常不表达。异源核酸和蛋白质也可以被称作外来核酸或蛋白质。在本文中术语异源核酸或蛋白质包括本领域技术人员会识别为对于下述细胞是异源或外源的任何核酸或蛋白质，所述核酸或蛋白质在所述细胞中被表达。
根据本发明的方法可以使用生物进行，所述生物可以是宿主生物，尤其是宿主微生物，或者野生型微生物。因此，本发明还涉及新颖的(宿主)细胞，其可以是微生物，包含能够催化本发明方法中至少一个反应步骤的生物催化剂，优选地，所述细胞能够生产催化本发明方法中的一个或多个反应步骤的酶或多种酶。本发明还涉及新颖的载体，所述载体包含编码一种或多种能够催化本发明方法中至少一个反应步骤的酶的一个或多个基因。
在一个实施方案中，提供了包含下述核酸序列的细胞或载体，所述核酸序列可以是重组的，编码具有5-羧基-2-戊烯基酯或硫代酯氢化酶活性、尤其是5-羧基-2-戊烯基氢化酶活性的酶。
优选地，细胞还包含至少一个编码选自下组的酶的核酸序列(包含所述核酸序列的重组载体)：能够催化己二酰酯或硫代酯、尤其是己二酰-CoA转化成为5-FVA的酶，和催化己二酰酯或硫代酯、尤其是己二酰-CoA转化成己二酸的酶。
具体地，在其中细胞包含能够催化己二酰酯或硫代酯转化成5-FVA的酶的一个实施方案中，细胞可有利地包含编码下述酶的核酸序列(包含所述序列的重组载体)，所述酶能够催化5-FVA转化成为6-ACA。这类酶可尤其是具有5-FVA氨基转移酶活性的酶。
另外或替代性地，(宿主)细胞和载体分别包含至少一种以下核酸序列：
-编码下述酶的核酸序列，所述酶能够通过使琥珀酰酯或硫代酯与乙酸酯或硫代酯反应来催化3-氧代己二酰酯或硫代酯的形成；
-编码下述酶的核酸序列，所述酶能够催化从3-氧代己二酰酯或硫代酯形成3-羟基己二酰酯或硫代酯；
-编码下述酶的核酸序列，所述酶能够催化从3-羟基己二酰酯或硫代酯形成5-羧基-2-戊烯基酯或硫代酯；
-编码下述酶的核酸序列，所述酶能够催化从5-羧基-2-戊烯基酯或硫代酯形成己二酰酯或硫代酯。
一个或多个合适的基因可尤其选自编码上述酶的基因，更尤其选自编码根据SEQUENCE ID 1-67、94、96、98、100、102、103、105、107、109、111、113、115、116任一的酶的基因或其同源物。
宿主细胞可以是原核生物或真核生物。宿主细胞尤其可选自细菌、古细菌、酵母、真菌、原生生物、植物和动物(包括人)。
具体地，根据本发明的宿主细胞可选自由Aspergillus、Bacillus、Corynebacterium、Escherichia、Saccharomyces、Pseudomonas、Gluconobacter、Penicillium、Pichia属组成的组。具体地，宿主菌株和由此得到的宿主细胞可以选自E.coli、Bacillus subtilis、Bacillus amyloliquefaciens、Corynebacteriumglutamicum、Aspergillus niger、Penicillium chrysogenum、Pichia pastoris、Saccharomyces cerevisiae的组。
能够生产短链脂肪酸如琥珀酸酯和/或乙酸酯和/或其酯或硫代酯的宿主细胞可以是有利的。能够进行这些的生物通常存在于反刍类的瘤胃中。具体地，能够共同生产琥珀酸酯和乙酸酯或其酯或硫代酯的生物是优选的。
微生物可以是重组的或野生型。具体地，能够生产琥珀酸酯的微生物包括E.coli、Actinobacillus(尤其是A.succinogenes)、Mannheimia(尤其是M.succiniciproducens)、Saccharomyces cerevisiae、Aspergillus(尤其是A.niger)、Penicillium(尤其是P.chrysogenum和P.simplicissimum)和Kaemwich Jantama,M.J.Haupt,SpyrosA.Svoronos,Xueli Zhang,J.C.Moore,K.T.Shanmugam,L.O.Ingram.Biotechnology andBioengineering(2007)99,5:1140-1153中提到的其它微生物。
具体地，能够生产乙酸酯的微生物包括Enterobacteriaceae(尤其是E.coli、Salmonella和Shigella)，乙酸细菌包括Acetobacter(尤其是Acetobacter aceti)、Gluconobacter(尤其是Gluconobacter oxidans)、Acidomonas、Gluconacetobacter、Asaia、Kozakia、Swaminathania、Saccharibacter、Neoasaia、Granulibacter、Clostridium(尤其是C.aceticum、C.thermoaceticum、C.thermoautotrophicum、C.formicoaceticum、C.kluyveri、C.propionicum)、Megasphaera(尤其是M.elsdenii)、Acetobacterium(尤其是A.woodii和A.wieringae)、Lactobacillus(尤其是L.plantarum、L.brevum)、Bifidobacterium(尤其是B.bifidum)和Leuconostoc。
本发明还涉及新颖的多肽，编码这类多肽的各核苷酸序列。具体地，本发明还涉及包含根据SEQUENCE ID 57、68-72、79、85任一的氨基酸序列及其同源物的多肽。具体地，本发明还涉及编码下述多肽的多核苷酸，所述多肽包含根据SEQUENCE ID 57、68-72、79、85任一的氨基酸序列及其同源物。
附图说明
图1示出了从琥珀酸酯或硫代酯制备己二酸酯或硫代酯。
图2示出了克隆策略的综述。仅展示了相关特征和限制性位点。
图3示出了己二酸(酯)生物合成通路。
图4示出了己二酸酯通路装配的克隆策略。
具体实施方式
接着，本发明将通过以下实施例阐述。
实施例
实施例1：一般方法
分子和遗传技术
标准遗传和分子生物学技术是本领域普遍已知的，并且先前已被描述(Maniatiset al.1982“Molecular cloning:a laboratory manual”.Cold Spring HarborLaboratory，Cold Spring Harbor，N.Y.；Miller 1972“Experiments in moleculargenetics”，Cold Spring Harbor Laboratory，Cold Spring Harbor；Sambrook andRussell 2001"Molecular cloning:a laboratory manual”(3rd edition)，Cold SpringHarbor Laboratory，Cold Spring Harbor Laboratory Press；F.Ausubel et al，eds.，"Current protocols in molecular biology"，Green Publishing and WileyInterscience，New York 1987)。
质粒和菌株
pBAD/Myc-His C和pET21d分别得自Invitrogen(Carlsbad，CA，USA)和EMDBiosciences(Darmstadt，德国)。pF113(pJF119EH(Fürste，J.P.，W.Pansegrau，R.Frank，H.Blocker，P.Scholz，M.Bagdasarian，and E.Lanka.1986.Molecular cloning of theplasmid RP4primase region in a multi-host-range tacP expression vector.Gene48:119-131.)的衍生物，其分别在515和5176位含有两个NotI位点，以tac启动子作为编号起点)，pACYC-tac(M.(2000).Untersuchungen zum EinflussBereitstellung von Erythrose-4-Phosphat und Phosphoenolpyruvat auf denKohlenstofffluss in den Aromatenbiosyntheseweg von Escherichia coli.Berichtedes Forschungszentrums Jülich，3824.ISSN 0944-2952(PhD Thesis，University of Düsseldorf)和pMS470(Balzer，D.；Ziegelin，G.；Pansegrau，W.；Kruft，V.；Lanka，E.NucleicAcids Research 1992，20(8)，1851-1858.)先前已被描述。E.coli TOP10(Invitrogen，Carlsbad，CA，USA)被用于所有克隆程序。E.coli菌株Top10(Invitrogen，Carlsbad，CA，USA)、Rv308(ATCC31608)、Rv308ΔaraB和BL21A1(Invitrogen，Carlsbad，CA，USA)被用于蛋白质表达。
通过插入常见连接子以允许相同的克隆策略来改造所有载体。改造和一般克隆流程展示于图2中。
培养基
2xTY培养基(16g/l tryptopeptone、10g/l酵母提取物、5g/l NaCl)被用于培养E.coli。补充抗生素(100μg/ml氨苄西林)来维持质粒。为了诱导基因表达，以0.005-0.2％(阿拉伯糖)和0.1-0.5mM(IPTG)的终浓度使用阿拉伯糖(对pBAD衍生物而言)、IPTG(对pMS470和pF113衍生物而言)、和阿拉伯糖与IPTG的组合(对E.coli BL21-A中的pET21d衍生物而言)。
质粒的鉴定
通过本领域普遍已知的遗传、生物化学和/或表型手段鉴定带有不同基因的质粒，如转化体对抗生素的抗性，转化体的PCR诊断性分析或质粒DNA的纯化，经纯化的质粒DNA的限制性分析或DNA序列分析。
用于CoA衍生物测定的HPLC-MS分析方法
通过LC-MS测定己二酰基-Coa和6-羧基-2,3-烯己酰-CoA的浓度。使用AgilentSB-C18 2.1*50mm柱进行分离，使用乙腈/经750mg/l辛烷基乙酸铵(pH＝7.5)缓冲的水作为流动相。流速为300μl/分钟，并用梯度(起始：70％水，在3分钟内降至58％，逐步达到45％，在1.5分钟内进一步降低至20％，之后再平衡柱使得总运行时间为7分钟)进行洗脱。以电喷射负离子化模式使用LTQ orbitrap从m/z 765-900进行扫描。己二酰基-Coa和6-羧基-2,3-ene烯己酰-CoA分别在2.25分钟和2.5分钟被洗脱。通过观察要求的化合物的精确质子化分子来提高所述方法的灵敏度(己二酰基-Coa：894.15123-894.16017，6-羧基-2,3-烯己酰-CoA：892.13437-892.14329)。为了测定浓度，运行合成制备的化合物的标准曲线，来计算各种离子的反应因子。这被用于计算未知样品中的浓度。
可如Kippenberger，M.；Winterhalter，R.；Moortgat，G.K.Anal.Bioanal.Chem.2008，392(7-8)，1459-1470中所述检测和定量己二酸
酯。实施例2
6-羧基-2,3-烯-己酰-CoA还原酶活性测定
表达构建体
推定的6-羧基-2,3-烯-己酰-CoA还原酶选自数据库(表1)。
编码所选蛋白质的靶基因(使用WO08000632中所述方法)进行了密码子对优化并合成构建(Geneart，Regensburg，德国)。优化前，从氨基酸序列中去除靶向序列(例如分泌信号或过氧化物酶体/线粒体靶向序列)。可以通过如Emanuelsson et al.2007.Natureprotocols，2:953-971中所述的本领域公知的生物信息学工具鉴定这类靶向序列。在优化程序中避免内部限制性位点，并在起点和终点处引入常见的限制性位点，以允许根据图2所示策略进行克隆。这些修饰可导致各个蛋白质序列的微小改变，期望这些改变不会以任何方式改变各个蛋白质的特性。每个ORF前是一致的核糖体结合位点和前导序列，以驱动在pF113、pMS470和pET21d中翻译。在pBAD中，由载体提供翻译起始信号。靶序列“Adi4”、“Adi5”、“Adi8”和“Adi9”以两种方式被克隆进所有四种质粒中：通读或不通读至连接子序列提供的C-端His-标签。
E.coli中的蛋白质表达
起始培养物在含200μl培养基/孔的96-孔平板中过夜培养。将40-160μl转移至具有4ml培养基的新鲜24-深孔平板中。对E.coli TOP10或E.coli Rv308ΔaraB中的pBAD构建体而言，该培养基直接含有0.005％阿拉伯糖用于诱导。将平板在轨道摇床(Infors，550rpm)上于25℃下孵育。4-6小时后添加诱导剂(对E.coli Rv308、E.coli BL21或E.coliTOP10中的pF113和pMS470而言为0.5mM IPTG；对E.coli BL21A1中的pET21d而言为0.5mMIPTG和0.2％阿拉伯糖)，并将平板再孵育4-48小时，直至通过离心收集细胞。
无细胞提取物的制备和His-标签纯化
通过离心收获来自小规模培养(见前一段)的细胞，弃去上清液。将离心期间形成的细胞沉淀物于-20℃下冷冻至少16下式，然后冰上融化。向每个孔中添加2ml新鲜制备的裂解缓冲液(50mM磷酸钾pH7.5、0.1mg/ml DNAse I(Roche，Almere，NL)、2mg/ml Lysozyme、0.5mM MgSO4、1mM二硫苏糖醇和蛋白酶抑制剂(根据制造商的说明书使用Complete MiniEDTA-free tabletsTM，Roche，Almere，NL))，并通过将平板剧烈振荡2-5分钟使细胞重悬。为了实现裂解，将平板在室温下孵育30分钟。为了去除细胞碎片，将平板在4℃和6000g下离心20分钟。将上清液转移至新鲜平板并置于冰上，直至再次使用。为了纯化带有His标签的蛋白质，根据制造商的说明书使用His Multitrap HP过滤平板(GE Healthcare bioscienceAB，Uppsala，瑞典)。
底物的合成
想要的生物化学反应的底物(J.R.Stern，A.del Campillo，J.Biol.Chem.，1956，985.A.K.Das，M.D.Uhler，A.K.Hajra，J.Biol.Chem.，2000，24333.H.Oku，N.Futamori，K.Masuda，Y.Shimabukuro，T.Omine，H.Iwasaki，Biosc.Biotech.Biochem.，2003，2107.Elvidge et.al，J.Chem.Soc.，1953，1793.F.Liu，H-Y.Zha，Z-J.Yao，J.Org.Chem.，2003，6679-6684)和产物(WO2004/106347)由Syncom(Groningen，NL)根据公开的程序合成。
酶促烯酰烯酰-CoA实验
制备包含50mM钾缓冲液(pH7.5)、NADH和NADPH各0.7mM，和约20μM底物6-羧酸2,3-烯己酰-CoA的反应混合物。向96孔板的每个孔中分散190μl反应混合物。以同样的方式，在对照反应中使用从带有空载体的各个菌株中制备的10μl无细胞提取物。向每个孔中添加10μl无细胞提取物或经纯化的蛋白质来起始反应。将反应混合物在室温(20-25℃)下孵育15分钟到24小时，联机监测340nm下的UV吸收。结束时通过添加等体积的MeOH终止反应并对样品离心。将上清液转移至新鲜平板并储存于-80℃下，直至通过HPLC-MS进一步分析。发现己二酰基-Coa如表1中所示。
表1：在酶促实验中找到的己二烯-CoA(量由相对峰面积指出)。
生物催化剂 SeqID# 修饰<sup>1</sup> 己二烯-CoA<sup>2</sup>
Adi4 63 去除N-端134个氨基酸 3266
Adi5 96 196031
Adi8 60 去除N-端22个氨基酸 581859
Adi9 100 去除N-端12个氨基酸 117077
-(载体对照) 0
1如果修饰后得到的多肽在N-端不以甲硫氨酸起始，则通过在N-端添加甲硫氨酸进一步修饰得到的多肽。
2显示的结果用孵育20小时后的下述E.coli BL21获得，所述E.coli BL21含有克隆在pMS470中的adi基因。用其它表达载体和宿主菌株(如E.coli BL21-A1中的pET21d，E.coli Rv308中的pBAD/Myc-His C和E.coli BL21中的pF113)和不同的孵育时间(例如2小时)也获得了阳性结果。
实施例3
通过异源微生物生产己二酸酯
己二酸酯生物合成通路的构建
设计由图3所示酶活性组成的合成通路。编码这些活性的酶在数据库中鉴定。编码这些酶的靶基因经密码子对优化并且合成构建(Geneart，Regensburg，德国)。在优化程序中去除内部限制性位点、不想要的靶向序列(例如分泌信号或过氧化物酶体靶向序列)并在起点和终点处引入限制性位点，以允许在根据图4的表达载体中装配所述通路。这些修饰可导致蛋白质序列的微小改变，期望这些改变不会以任何方式改变各个蛋白质的特性。每个ORF前是一致的核糖体结合位点和前导序列，以驱动翻译。
对反应1、2和3而言，使用adi21+22+23或adi26+27+28的组合。对反应7而言，使用adi29、adi30或adi24+25的组合。对反应4而言，可以与adi8、adi6、adi13+12一起使用adi1-20。
表2：不同Adi蛋白质的SeqIDs。
蛋白质 Seq ID#
Adi21 5
Adi22 18
Adi23 33
Adi24 119
Adi25 120
Adi26 3
Adi27 29
Adi28 14
Adi29 116
Adi30 117
生产己二酸酯的E.coli菌株的构建
为了构建生产己二酸酯的E.coli菌株，将编码整个己二酸酯通路的质粒转化进适当的宿主菌株中，用于表达被克隆的基因。将含有完整通路的pMS470构建体转化进E.coliBL21、TOP10和Rv308中。将pBAD/Myc-His C构建体转化进E.coli TOP10和Rv308ΔaraB中。与相容的pF113、pACYC-tac或pMS470构建体一起以下述方式转化pF113或pACYC-tac中的构建体，所述方式使得最终菌株含有整个己二酸酯通路。将这些质粒共转化至E.coli TOP10、BL21和Rv308。
己二酸酯的生产
为了生产己二酸酯，将起始培养物在含200μl培养基的96-孔平板中于30℃下过夜培养。将50μl转移至含4ml培养基的新鲜的24孔平板中，在25℃下培养4-6小时，然后添加诱导己二酸酯通路表达的诱导剂。将培养物在25℃下再培养12-72小时。将平板离心并制备用于LC-MS分析的样品。将上清液与MeOH 1:1混合以沉淀蛋白质，然后直接分析。通过将沉淀物重悬于1ml乙醇中，提取来自细胞的代谢产物。将细胞悬浮液转移至带有螺旋盖的管，并在沸水浴中加热3分钟。离心后将上清液转移至新鲜的管，在高速真空(speed-vac)中蒸发。分析之前将干燥样品重悬于100μl流动相中。
实施例4
从己二酰-Coa制备5-FVA
用于5-FVA测定的HPLC-MS分析方法
通过选择性监测(SRM)-MS，测量m/z 129→83的跃迁来检测5-FVA。通过测量在约6分钟时洗脱的5-FVA峰的峰面积，计算5-FVA的浓度。通过使用外部标准流程进行校准。所有LC-MS实验在Agilent 1200LC系统上进行，所述Agilent 1200LC系统由四元泵、自动取样机和柱温箱组成，与Agilent 6410QQQ三重四极杆质谱仪(triple quadrupole MS)偶联。
LC条件：
柱：每个柱50x 4.6mm Nucleosil C18，5μm(Machery&Nagel)，与250x 4.6mmid.Prevail C18，5μm(Alltech)偶联
柱温度：室温
洗脱液：A：含0.1％甲酸的水
B：含0.1％甲酸的乙腈
流速：1.2ml/分钟，进入MS之前1:3分流
注射体积：2μl
MS条件：
电离：负离子电喷射
来源条件：离子喷射电压：5kV
温度：350℃
碎裂电压(fragmentor voltage)和碰撞能量经优化
扫描模式：选择性反应模式：跃迁m/z 129→83
表达构建体
选择推定的己二酰-CoA-还原酶(SeqID 74、75、77、79、80、139-148)。以与前文实施例2中所述相同的方式，使用pBAD/Myc-His C和pET21d设计并制备表达构建体。
蛋白质表达、提取和纯化
所有步骤如实施例2中所述进行。
酶促己二酰-CoA还原酶实验
制备包含50mM钾缓冲液(pH7.5)、NADH和NADPH各0.7mM，和10μM-10mM底物己二酰-CoA的反应混合物。向96孔板的每个孔中分散190μl反应混合物。如实施例2中所述制备己二酰-CoA。向每个孔中添加10μl无细胞提取物或经纯化的蛋白质来起始反应。以同样的方式，在对照反应中使用从带有空载体的各个菌株中制备的10μl无细胞提取物。将反应混合物在室温(20-25℃)下孵育15分钟到24小时，联机监测340nm下的UV吸收。结束时通过添加等体积的MeOH终止反应并对样品离心。将上清液转移至新鲜平板并储存于-80℃下，直至通过HPLC-MS检测5-FVA。5-FVA的测量证明所选择的酶的己二酰基-CoA还原酶活性。
实施例5
从5-FVA制备6-ACA
用于测定6-ACA的HPLC-MS分析
校准：
通过6-ACA的外部校准曲线进行校准(m/z 132→m/z 114，Rt 7.5分钟)。所有LC-MS实验在Agilent 1100上进行，所述Agilent 1100装有四元泵、除气器、自动取样机、柱温箱和单四极杆MS(Agilent，Waldbronn，德国)。LC-MS条件为：
柱：50*4Nucleosil(Mancherey-Nagel)+250x 4.6Prevail C18(Alltech)，均在室温(RT)下
洗脱液：A＝超纯水中0.1(v/v)甲酸
B＝乙腈(pa，Merck)
流速：1.0ml/分钟，进入MS之前1:3分流
梯度：梯度在t＝0分钟时以100％(v/v)A开始，保持15分钟，并在15分钟内改变至80％(v/v)B(t＝30分钟)。从30到31分钟时，将梯度保持恒定在80％(v/v)B。
注射体积：5μl
MS检测：ESI(+)-MS
电喷射电离(ESI)在正扫描模式下使用以下条件运行；m/z 50-500，50V碎裂电压，0.1m/z步长，350℃干燥气体温度，10L N2/分钟干燥气体，50psig雾化器压强和2.5kV毛细管电压。
靶基因的克隆
表达构建体的设计
对核糖体结合位点和起始密码子上游和终止密码子下游的所有基因添加attB位点，以便使用Gateway技术(Invitrogen，Carlsbad，CA，USA)进行克隆。
基因合成和质粒构建
根据DNA2.0的标准程序，从DNA2.0获得合成的基因并为了在E.coli中表达进行密码子优化。对分别编码V.fluvialis JS17ω-氨基转移酶[SEQ ID No.82]和B.weihenstephanensis KBAB4氨基转移酶(ZP_01186960)[SEQ ID No.83]的氨基酸序列的，来自Vibrio fluvialis JS17[SEQ ID No.86]和Bacillus weihenstephanensis KBAB4[SEQ ID No.89]的氨基转移酶基因进行密码子优化，并通过DNA合成获得得到的序列[SEQID No.88]和[SEQ ID No.91]。
用所述基因提供的细胞在下文中分别被称作E.coli TOP10/pBAD-Vfl_AT和E.coli TOP10/pBAD-Bwe_AT。
通过PCR克隆
根据制造商的说明书使用PCR Supermix High Fidelity(Invitrogen)，通过PCR从基因组DNA中扩增编码生物催化剂的多个基因。
通过琼脂糖凝胶电泳分析PCR反应，使用QIAquick PCR纯化试剂盒(Qiagen，Hilden，德国)从凝胶中洗脱具有正确大小的PCR产物。如制造商方案中所述，使用Gateway技术(Invitrogen)，通过引入的attB位点和pDONR-zeo(Invitrogen)作为进入载体，将经纯化的PCR产物克隆进pBAD/Myc-His-DEST表达载体中。通过DNA测序验证通过PCR克隆的基因序列。藉此获得了表达载体pBAD-Bsu_gi16077991_AT(包含SEQUENCE ID 133所示基因，所述基因编码SEQUENCE ID 134所示肽)、pBAD-Pae_gi9946143_AT(使用SEQUENCE ID 130和131中鉴定的引物)、pBAD-Pae_gi9951072_AT(包含SEQUENCE ID 135所示基因，所述基因编码SEQUENCE ID 136所示肽)、pBAD-Pae_gi9951630_AT(包含SEQUENCE ID 137所示基因，所述基因编码SEQUENCE ID 138所示肽)。通过用pBAD构建体转化化学感受态E.coli TOP10(Invitrogen)获得相应的表达菌株。
将5-甲酰戊酸转化为6-ACA的酶促反应
除非另有说明，制备在50mM磷酸钾缓冲液，pH 7.0中包含10mM 5-甲酰戊酸、20mM外消旋α-甲基苄胺和200μM5’-磷酸吡哆醛的反应混合物。向带孔平板的每个孔中分散100μl反应混合物。向每个孔中添加20μl无细胞提取物。将反应混合物在摇床上于37℃下孵育24小时。另外，在相同条件下孵育化学空白混合物(不含无细胞提取物)和生物空白(带有pBAD/Myc-His C的E.coli TOP10)。通过HPLC-MS分析样品。结果概括于下表中。
表3：在存在氨基转移酶时从5-FVA形成6-ACA
*方法的差异在于使用10μl无细胞提取物而不是20μl，5’-磷酸吡哆醛浓度是50μM而不是200μM，并且孔中的反应混合物体积为190μl而不是100μl
显示在存在氨基转移酶时从5-FVA形成6-ACA，并且E.coli能够催化所述形成。
序列表
<110> 基因组股份公司
<120> 己二酸酯或硫代酯合成
<130> P84199PC00
<150> EP 08152595.8
<151> 2008-11-03
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Ile Ala Asn Ala Ile Gln Ser Gly Met Ile Asp Val Gly Ile Gly Ala
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Gly Val Glu Ser Met Ser Ser Gln Tyr Gly Pro Gly Ala Val Thr Glu
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Phe Ser Asp Leu Leu Glu Ser His Pro Glu Ser Ala Asn Cys Lys Val
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Pro Met Gly Val Leu Ser Glu Asn Met Ala Lys Asp Arg Gly Val Thr
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Arg Ala Ser Gln Asp Ser Phe Ala Ala Gln Ser Tyr Gln Lys Ala Val
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Ala Ala Gln Lys Ala Gly Leu Phe Asn Glu Glu Ile Ala Pro Leu Asp
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Val Lys Trp Thr Asp Pro Lys Thr Gly Glu Glu Lys Thr Ile Thr Val
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Asp Pro Lys Thr Gly Glu Val Lys Asp Ile Val Val Val Arg Asp Asp
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His Pro Arg Pro Ser Ser Thr Leu Glu Ala Leu Ser Lys Leu Lys Pro
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Leu Phe Glu Gly Gly Val Val Thr Ala Gly Asn Ala Ser Gly Ile Asn
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Asp Gly Ala Ala Ala Leu Leu Ile Gly Ser Glu Val Ala Gly Gln Lys
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Tyr Gly Leu Thr Pro Met Ala Lys Ile Leu Ser Ala Ala Ala Ala Gly
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Val Glu Pro Arg Ile Met Gly Ala Gly Pro Ile Glu Ala Ile Lys Lys
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Ala Val Ala Arg Ala Gly Leu Thr Leu Asp Asp Leu Asp Ile Ile Glu
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Ile Asn Glu Ala Phe Ala Ser Gln Val Leu Ser Cys Leu Lys Gly Leu
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Gly Ile Asp Phe Asn Asp Pro Arg Val Asn Pro Asn Gly Gly Ala Ile
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Ala Val Gly His Pro Leu Gly Ala Ser Gly Ala Arg Leu Ala Leu Thr
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Val Ala Arg Glu Leu Gln Arg Arg Asn Lys Lys Tyr Ala Val Val Ser
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Leu Cys Ile Gly Val Gly Gln Gly Leu Ala Met Val Ile Glu Asn Val
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Ser
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Ala Phe Thr Val Asn Lys Leu Cys Gly Ser Gly Leu Arg Thr Val Ser
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Leu Ala Ala Ser Leu Ile Ser Asn Gly Asp Ala Asp Thr Ile Val Val
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His Asp Gly Leu Ile Asp Ser Phe Asn Asn Tyr His Met Gly Ile Thr
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225 230 235 240
Gly Thr Val Thr Ala Gly Asn Ser Ser Gly Ile Asn Asp Ser Ser Ala
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260 265 270
Pro Met Ala Lys Tyr Val Asp Phe Ala Ser Ala Gly Leu Asp Pro Ala
275 280 285
Ile Met Gly Tyr Gly Pro Tyr Tyr Ala Thr Lys Lys Val Leu Ala Lys
290 295 300
Thr Asn Leu Thr Ile Lys Asp Phe Asp Leu Ile Glu Ala Asn Glu Ala
305 310 315 320
Phe Ala Ala Gln Ser Ile Ala Val Ala Arg Asp Leu Glu Phe Asp Met
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Ser Lys Val Asn Val Asn Gly Gly Ala Ile Ala Leu Gly His Pro Val
340 345 350
Gly Cys Ser Gly Ala Arg Ile Leu Val Thr Leu Leu His Glu Met Gln
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Lys Arg Asp Ala Lys Lys Gly Leu Ala Thr Leu Cys Ile Gly Gly Gly
370 375 380
Gln Gly Thr Ala Val Val Val Glu Arg
385 390
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Met Lys Asp Ala Val Ile Val Ser Ala Val Arg Thr Ala Ile Gly Ser
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Phe Gly Gly Thr Leu Lys Asp Ile Ser Ala Val Asp Leu Gly Ala Ile
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Val Ile Lys Glu Ala Val Lys Arg Ala Gly Ile Lys Pro Glu Gln Val
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Asp Glu Val Ile Phe Gly Asn Val Ile Gln Ala Gly Leu Gly Gln Ser
50 55 60
Pro Ala Arg Gln Ala Ala Val Lys Ala Gly Ile Pro Val Glu Val Pro
65 70 75 80
Ala Phe Thr Leu Asn Lys Val Cys Gly Ser Gly Leu Arg Ser Val Ser
85 90 95
Leu Ala Ala Gln Leu Ile Lys Ile Gly Asp Asp Asp Ile Val Val Val
100 105 110
Gly Gly Thr Glu Asn Met Ser Ala Ala Pro Tyr Leu Leu Pro Lys Ala
115 120 125
Arg Trp Gly His Arg Met Gly Glu Gly Lys Leu Val Asp Ala Met Ile
130 135 140
Lys Asp Gly Leu Trp Glu Ala Phe Asn Asn Tyr His Met Gly Ile Thr
145 150 155 160
Ala Glu Asn Ile Ala Glu Lys Trp Gly Ile Thr Arg Asp Met Gln Asp
165 170 175
Glu Phe Ala Leu Ala Ser Gln Gln Lys Ala Glu Ala Ala Ile Lys Ala
180 185 190
Gly Lys Phe Lys Asp Glu Ile Val Pro Val Thr Val Lys Gln Lys Lys
195 200 205
Lys Glu Ile Ile Phe Asp Thr Asp Glu Phe Pro Arg Phe Gly Thr Thr
210 215 220
Ile Glu Ala Leu Ala Lys Leu Lys Pro Ser Phe Lys Lys Asp Gly Thr
225 230 235 240
Val Thr Ala Gly Asn Ala Ser Gly Ile Asn Asp Ala Ala Ala Ala Leu
245 250 255
Val Val Met Ser Ala Asp Lys Ala Lys Glu Leu Gly Ile Lys Pro Leu
260 265 270
Ala Lys Ile Val Ser Tyr Gly Ser Lys Gly Leu Asp Pro Thr Ile Met
275 280 285
Gly Tyr Gly Pro Phe Tyr Ala Thr Lys Leu Ala Leu Glu Lys Ala Asn
290 295 300
Leu Ser Ile Ala Asp Leu Asp Leu Ile Glu Ala Asn Glu Ala Phe Ala
305 310 315 320
Ser Gln Ser Leu Ala Val Ala Lys Asp Leu Glu Phe Asp Met Ser Lys
325 330 335
Val Asn Val Asn Gly Gly Ala Ile Ala Leu Gly His Pro Val Gly Cys
340 345 350
Ser Gly Ala Arg Ile Leu Val Thr Leu Leu Tyr Glu Met Gln Arg Arg
355 360 365
Asp Ala Lys Lys Gly Leu Ala Thr Leu Cys Ile Gly Gly Gly Met Gly
370 375 380
Thr Ala Leu Ile Val Glu Arg
385 390
<210> 8
<211> 391
<212> PRT
<213> Clostridium kluyveri
<400> 8
Met Arg Glu Val Val Ile Val Ser Ala Val Arg Thr Ala Ile Gly Ser
1 5 10 15
Phe Gly Gly Thr Leu Lys Asp Val Pro Ala Val Glu Leu Gly Ala Val
20 25 30
Val Ile Lys Glu Ala Val Lys Arg Ala Asn Val Lys Pro Glu Gln Ile
35 40 45
Asp Glu Val Ile Phe Gly Asn Val Ile Gln Ala Gly Leu Gly Gln Ser
50 55 60
Pro Ala Arg Gln Ala Ala Val Lys Ala Gly Ile Pro Val Glu Val Pro
65 70 75 80
Ala Phe Thr Leu Asn Lys Val Cys Gly Ser Gly Leu Arg Ser Val Ser
85 90 95
Leu Ala Ala Gln Val Ile Lys Ala Gly Asp Ala Asp Ile Val Val Val
100 105 110
Gly Gly Met Glu Asn Met Ser Ala Ala Pro Tyr Val Leu Pro Lys Ala
115 120 125
Arg Trp Gly His Arg Met Gly Glu Gly Lys Ile Val Asp Ala Met Ile
130 135 140
Lys Asp Gly Leu Trp Glu Ala Phe Asn Asn Tyr His Met Gly Ile Thr
145 150 155 160
Ala Glu Asn Ile Ala Glu Lys Trp Gly Leu Thr Arg Glu Glu Gln Asp
165 170 175
Glu Phe Ser Ala Ala Ser Gln Gln Lys Ala Glu Ala Ala Gln Lys Ala
180 185 190
Gly Lys Phe Lys Asp Glu Ile Val Pro Val Thr Val Lys Ile Lys Lys
195 200 205
Lys Glu Val Val Phe Asp Thr Asp Glu Tyr Ile Lys Pro Gly Thr Thr
210 215 220
Val Glu Thr Leu Ala Lys Leu Arg Pro Ala Phe Lys Lys Asp Gly Thr
225 230 235 240
Val Thr Ala Gly Asn Ala Ser Gly Ile Asn Asp Ala Ala Ala Ala Leu
245 250 255
Val Val Met Ser Ala Asp Lys Ala Lys Glu Leu Gly Ile Lys Pro Leu
260 265 270
Ala Lys Ile Val Ser Tyr Gly Ser Ala Gly Leu Asp Pro Thr Ile Met
275 280 285
Gly Tyr Gly Pro Phe His Ala Thr Lys Ala Ala Leu Glu Lys Ala Asn
290 295 300
Leu Ser Val Ala Asp Leu Asp Leu Ile Glu Ala Asn Glu Ala Phe Ala
305 310 315 320
Ser Gln Ser Leu Ala Val Ala Lys Asp Leu Glu Phe Asp Met Ser Lys
325 330 335
Val Asn Val Asn Gly Gly Ala Ile Ala Leu Gly His Pro Val Gly Ala
340 345 350
Ser Gly Ala Arg Ile Leu Val Thr Leu Leu His Glu Met Gln Arg Arg
355 360 365
Asp Ala Lys Lys Gly Leu Ala Thr Leu Cys Ile Gly Gly Gly Met Gly
370 375 380
Thr Ala Leu Ile Val Glu Arg
385 390
<210> 9
<211> 408
<212> PRT
<213> Corynebacterium glutamicum
<400> 9
Met Asn Pro Gln Asp Ile Val Ile Cys Ser Pro Leu Arg Thr Pro Val
1 5 10 15
Gly Ala Tyr Gly Gly Ser Phe Thr Gly Val Pro Val Glu Glu Leu Ala
20 25 30
Thr Thr Val Ile Asn Ala Ile Val Glu Ala Thr Gly Ile Thr Gly Asp
35 40 45
Asp Val Asp Asp Leu Ile Leu Gly Gln Ala Ser Pro Asn Gly Ala Ala
50 55 60
Pro Ala Leu Gly Arg Val Val Ala Leu Asp Ser Lys Leu Gly Gln Asn
65 70 75 80
Val Pro Gly Met Gln Leu Asp Arg Arg Cys Gly Ser Gly Leu Gln Ala
85 90 95
Ile Val Thr Ala Ala Ala His Val Ala Ser Gly Ala Ala Asp Leu Ile
100 105 110
Ile Ala Gly Gly Ala Glu Ser Met Ser Arg Val Glu Tyr Thr Val Ser
115 120 125
Gly Asp Ile Arg Trp Gly Val Lys Gly Gly Asp Met Gln Leu Arg Asp
130 135 140
Arg Leu Ala Glu Ala Arg Glu Thr Ala Gly Gly Arg Asn His Pro Ile
145 150 155 160
Pro Gly Gly Met Ile Glu Thr Ala Glu Asn Leu Arg Arg Glu Tyr Gly
165 170 175
Ile Ser Arg Glu Glu Gln Asp Lys Ile Ser Ala Ala Ser Gln Gln Arg
180 185 190
Trp Gly Lys Ala Ala Asp Ala Gly Leu Phe Asp Asp Glu Ile Val Pro
195 200 205
Val Thr Val Pro Ala Lys Lys Arg Gly Gln Glu Pro Thr Ile Val Ser
210 215 220
Arg Asp Glu His Gly Arg Pro Gly Thr Thr Val Glu Lys Leu Ala Ala
225 230 235 240
Leu Arg Pro Ile Met Gly Arg Gln Asp Ala Glu Ala Thr Val Thr Ala
245 250 255
Gly Asn Ala Ser Gly Gln Asn Asp Gly Ala Ala Ala Val Ile Val Thr
260 265 270
Thr Arg Ala Lys Ala Glu Glu Lys Gly Leu Arg Pro Val Met Arg Leu
275 280 285
Ala Gly Trp Ser Val Ala Ala Val Pro Pro Glu Thr Met Gly Ile Gly
290 295 300
Pro Val Pro Ala Thr Lys Lys Val Leu Asp Arg Leu Gly Leu Thr Leu
305 310 315 320
Glu Asp Ile Gly Ala Ile Glu Leu Asn Glu Ala Phe Ala Ala Gln Ala
325 330 335
Leu Ser Val Leu Lys Glu Trp Asn Ile Ser Trp Glu Asp Glu Arg Val
340 345 350
Asn Pro Leu Gly Ser Gly Ile Ser Met Gly His Pro Val Gly Ala Thr
355 360 365
Gly Ala Arg Met Ala Val Thr Leu Ala His Arg Met Gln Arg Glu Asn
370 375 380
Thr Gln Tyr Gly Leu Ala Thr Met Cys Ile Gly Gly Gly Gln Gly Leu
385 390 395 400
Ala Ala Val Phe Glu Lys Glu Asn
405
<210> 10
<211> 401
<212> PRT
<213> Escherichia coli
<400> 10
Met Arg Glu Ala Phe Ile Cys Asp Gly Ile Arg Thr Pro Ile Gly Arg
1 5 10 15
Tyr Gly Gly Ala Leu Ser Ser Val Arg Ala Asp Asp Leu Ala Ala Ile
20 25 30
Pro Leu Arg Glu Leu Leu Val Arg Asn Pro Arg Leu Asp Ala Glu Cys
35 40 45
Ile Asp Asp Val Ile Leu Gly Cys Ala Asn Gln Ala Gly Glu Asp Asn
50 55 60
Arg Asn Val Ala Arg Met Ala Thr Leu Leu Ala Gly Leu Pro Gln Ser
65 70 75 80
Val Ser Gly Thr Thr Ile Asn Arg Leu Cys Gly Ser Gly Leu Asp Ala
85 90 95
Leu Gly Phe Ala Ala Arg Ala Ile Lys Ala Gly Asp Gly Asp Leu Leu
100 105 110
Ile Ala Gly Gly Val Glu Ser Met Ser Arg Ala Pro Phe Val Met Gly
115 120 125
Lys Ala Ala Ser Ala Phe Ser Arg Gln Ala Glu Met Phe Asp Thr Thr
130 135 140
Ile Gly Trp Arg Phe Val Asn Pro Leu Met Ala Gln Gln Phe Gly Thr
145 150 155 160
Asp Ser Met Pro Glu Thr Ala Glu Asn Val Ala Glu Leu Leu Lys Ile
165 170 175
Ser Arg Glu Asp Gln Asp Ser Phe Ala Leu Arg Ser Gln Gln Arg Thr
180 185 190
Ala Lys Ala Gln Ser Ser Gly Ile Leu Ala Glu Glu Ile Val Pro Val
195 200 205
Val Leu Lys Asn Lys Lys Gly Val Val Thr Glu Ile Gln His Asp Glu
210 215 220
His Leu Arg Pro Glu Thr Thr Leu Glu Gln Leu Arg Gly Leu Lys Ala
225 230 235 240
Pro Phe Arg Ala Asn Gly Val Ile Thr Ala Gly Asn Ala Ser Gly Val
245 250 255
Asn Asp Gly Ala Ala Ala Leu Ile Ile Ala Ser Glu Gln Met Ala Ala
260 265 270
Ala Gln Gly Leu Thr Pro Arg Ala Arg Ile Val Ala Met Ala Thr Ala
275 280 285
Gly Val Glu Pro Arg Leu Met Gly Leu Gly Pro Val Pro Ala Thr Arg
290 295 300
Arg Val Leu Glu Arg Ala Gly Leu Ser Ile His Asp Met Asp Val Ile
305 310 315 320
Glu Leu Asn Glu Ala Phe Ala Ala Gln Ala Leu Gly Val Leu Arg Glu
325 330 335
Leu Gly Leu Pro Asp Asp Ala Pro His Val Asn Pro Asn Gly Gly Ala
340 345 350
Ile Ala Leu Gly His Pro Leu Gly Met Ser Gly Ala Arg Leu Ala Leu
355 360 365
Ala Ala Ser His Glu Leu His Arg Arg Asn Gly Arg Tyr Ala Leu Cys
370 375 380
Thr Met Cys Ile Gly Val Gly Gln Gly Ile Ala Met Ile Leu Glu Arg
385 390 395 400
Val
<210> 11
<211> 400
<212> PRT
<213> Pseudomonas putida
<400> 11
Met His Asp Val Phe Ile Cys Asp Ala Ile Arg Thr Pro Ile Gly Arg
1 5 10 15
Phe Gly Gly Ala Leu Ala Ser Val Arg Ala Asp Asp Leu Ala Ala Val
20 25 30
Pro Leu Lys Ala Leu Ile Glu Arg Asn Pro Gly Val Gln Trp Asp Gln
35 40 45
Val Asp Glu Val Phe Phe Gly Cys Ala Asn Gln Ala Gly Glu Asp Asn
50 55 60
Arg Asn Val Ala Arg Met Ala Leu Leu Leu Ala Gly Leu Pro Glu Ser
65 70 75 80
Ile Pro Gly Val Thr Leu Asn Arg Leu Cys Ala Ser Gly Met Asp Ala
85 90 95
Val Gly Thr Ala Phe Arg Ala Ile Ala Ser Gly Glu Met Glu Leu Val
100 105 110
Ile Ala Gly Gly Val Glu Ser Met Ser Arg Ala Pro Phe Val Met Gly
115 120 125
Lys Ala Glu Ser Ala Tyr Ser Arg Asn Met Lys Leu Glu Asp Thr Thr
130 135 140
Ile Gly Trp Arg Phe Ile Asn Pro Leu Met Lys Ser Gln Tyr Gly Val
145 150 155 160
Asp Ser Met Pro Glu Thr Ala Asp Asn Val Ala Asp Asp Tyr Gln Val
165 170 175
Ser Arg Ala Asp Gln Asp Ala Phe Ala Leu Arg Ser Gln Gln Lys Ala
180 185 190
Ala Ala Ala Gln Ala Ala Gly Phe Phe Ala Glu Glu Ile Val Pro Val
195 200 205
Arg Ile Ala His Lys Lys Gly Glu Ile Ile Val Glu Arg Asp Glu His
210 215 220
Leu Arg Pro Glu Thr Thr Leu Glu Ala Leu Thr Lys Leu Lys Pro Val
225 230 235 240
Asn Gly Pro Asp Lys Thr Val Thr Ala Gly Asn Ala Ser Gly Val Asn
245 250 255
Asp Gly Ala Ala Ala Met Ile Leu Ala Ser Ala Ala Ala Val Lys Lys
260 265 270
His Gly Leu Thr Pro Arg Ala Arg Val Leu Gly Met Ala Ser Gly Gly
275 280 285
Val Ala Pro Arg Val Met Gly Ile Gly Pro Val Pro Ala Val Arg Lys
290 295 300
Leu Thr Glu Arg Leu Gly Ile Ala Val Ser Asp Phe Asp Val Ile Glu
305 310 315 320
Leu Asn Glu Ala Phe Ala Ser Gln Gly Leu Ala Val Leu Arg Glu Leu
325 330 335
Gly Val Ala Asp Asp Ala Pro Gln Val Asn Pro Asn Gly Gly Ala Ile
340 345 350
Ala Leu Gly His Pro Leu Gly Met Ser Gly Ala Arg Leu Val Leu Thr
355 360 365
Ala Leu His Gln Leu Glu Lys Ser Gly Gly Arg Lys Gly Leu Ala Thr
370 375 380
Met Cys Val Gly Val Gly Gln Gly Leu Ala Leu Ala Ile Glu Arg Val
385 390 395 400
<210> 12
<211> 394
<212> PRT
<213> Ralstonia eutropha
<400> 12
Met Thr Arg Glu Val Val Val Val Ser Gly Val Arg Thr Ala Ile Gly
1 5 10 15
Thr Phe Gly Gly Ser Leu Lys Asp Val Ala Pro Ala Glu Leu Gly Ala
20 25 30
Leu Val Val Arg Glu Ala Leu Ala Arg Ala Gln Val Ser Gly Asp Asp
35 40 45
Val Gly His Val Val Phe Gly Asn Val Ile Gln Thr Glu Pro Arg Asp
50 55 60
Met Tyr Leu Gly Arg Val Ala Ala Val Asn Gly Gly Val Thr Ile Asn
65 70 75 80
Ala Pro Ala Leu Thr Val Asn Arg Leu Cys Gly Ser Gly Leu Gln Ala
85 90 95
Ile Val Ser Ala Ala Gln Thr Ile Leu Leu Gly Asp Thr Asp Val Ala
100 105 110
Ile Gly Gly Gly Ala Glu Ser Met Ser Arg Ala Pro Tyr Leu Ala Pro
115 120 125
Ala Ala Arg Trp Gly Ala Arg Met Gly Asp Ala Gly Leu Val Asp Met
130 135 140
Met Leu Gly Ala Leu His Asp Pro Phe His Arg Ile His Met Gly Val
145 150 155 160
Thr Ala Glu Asn Val Ala Lys Glu Tyr Asp Ile Ser Arg Ala Gln Gln
165 170 175
Asp Glu Ala Ala Leu Glu Ser His Arg Arg Ala Ser Ala Ala Ile Lys
180 185 190
Ala Gly Tyr Phe Lys Asp Gln Ile Val Pro Val Val Ser Lys Gly Arg
195 200 205
Lys Gly Asp Val Thr Phe Asp Thr Asp Glu His Val Arg His Asp Ala
210 215 220
Thr Ile Asp Asp Met Thr Lys Leu Arg Pro Val Phe Val Lys Glu Asn
225 230 235 240
Gly Thr Val Thr Ala Gly Asn Ala Ser Gly Leu Asn Asp Ala Ala Ala
245 250 255
Ala Val Val Met Met Glu Arg Ala Glu Ala Glu Arg Arg Gly Leu Lys
260 265 270
Pro Leu Ala Arg Leu Val Ser Tyr Gly His Ala Gly Val Asp Pro Lys
275 280 285
Ala Met Gly Ile Gly Pro Val Pro Ala Thr Lys Ile Ala Leu Glu Arg
290 295 300
Ala Gly Leu Gln Val Ser Asp Leu Asp Val Ile Glu Ala Asn Glu Ala
305 310 315 320
Phe Ala Ala Gln Ala Cys Ala Val Thr Lys Ala Leu Gly Leu Asp Pro
325 330 335
Ala Lys Val Asn Pro Asn Gly Ser Gly Ile Ser Leu Gly His Pro Ile
340 345 350
Gly Ala Thr Gly Ala Leu Ile Thr Val Lys Ala Leu His Glu Leu Asn
355 360 365
Arg Val Gln Gly Arg Tyr Ala Leu Val Thr Met Cys Ile Gly Gly Gly
370 375 380
Gln Gly Ile Ala Ala Ile Phe Glu Arg Ile
385 390
<210> 13
<211> 406
<212> PRT
<213> Rhodococcus sp.
<400> 13
Met Ala Glu Val Phe Leu Val Asp Gly Ala Arg Thr Pro Gln Gly Arg
1 5 10 15
Tyr Gly Gly Ala Leu Ala Gly Val Arg Pro Asp Asp Leu Ala Gly Leu
20 25 30
Val Val Ala Glu Ala Ala Arg Arg Ala Gly Ile Pro Gly Asp Ala Val
35 40 45
Asp Glu Val Ile Leu Gly Ala Ala Asn Gln Ala Gly Glu Asp Asn Arg
50 55 60
Asp Val Ala Arg Met Ala Val Leu Leu Ala Gly Leu Pro Asp Ser Val
65 70 75 80
Pro Gly Tyr Thr Val Asn Arg Leu Cys Ala Ser Gly Leu Thr Ala Val
85 90 95
Ala Ser Ala Ala His Thr Ile Arg Ser Gly Glu Ala Asp Ile Val Ile
100 105 110
Ala Gly Gly Val Glu Ser Met Thr Arg Ala Pro Trp Val Met Ala Lys
115 120 125
Pro Gly Thr Pro Trp Ala Arg Pro Gly Glu Val Ala Asp Thr Ser Leu
130 135 140
Gly Trp Arg Phe Thr Asn Pro Arg Phe Thr Ala Ala Asp Arg Asp Val
145 150 155 160
Pro Ala Gly Ala Gly Pro Asp Val Arg Lys Val Thr Leu Ser Met Gly
165 170 175
Glu Thr Ala Glu Glu Val Ala Ala Leu Glu Gly Val Thr Arg Ala Glu
180 185 190
Ser Asp Ala Phe Ala Leu Arg Ser Gln Glu Arg Ala Ile Ala Ala Val
195 200 205
Asp Ala Gly Arg Phe Glu Arg Glu Ile Val Pro Val Pro Val Arg Asp
210 215 220
Gly Glu Leu Ala Ala Asp Glu Thr Pro Arg Arg Gly Thr Thr Leu Glu
225 230 235 240
Lys Leu Gly Ser Leu Lys Pro Val Phe Arg Thr Gly Gly Ile Val Thr
245 250 255
Ala Gly Ser Ser Ser Ser Leu Ser Asp Gly Ala Ala Ala Leu Val Val
260 265 270
Ala Ser Glu Ala Ala Val Glu Lys Tyr Gly Leu Thr Val Arg Gly Arg
275 280 285
Ile Val Thr Ser Ala Ser Ala Gly Ile Ala Pro Asn Val Met Gly Leu
290 295 300
Gly Pro Val Pro Ala Thr Arg Lys Ala Leu Ala Arg Ala Asn Trp Ser
305 310 315 320
Ile Ser Asp Leu Gly Ala Ala Glu Leu Asn Glu Ala Phe Ala Ala Gln
325 330 335
Ser Leu Gly Val Ile Arg Gln Leu Lys Leu Asp Glu Ser Ile Val Asn
340 345 350
Ala Asp Gly Gly Ala Ile Ala Leu Gly His Pro Leu Gly Cys Ser Gly
355 360 365
Ala Arg Ile Leu Leu Thr Leu Leu Gly Arg Met Glu Arg Glu Gly Ala
370 375 380
Arg Arg Gly Leu Ala Thr Leu Cys Val Gly Val Gly Gln Gly Val Ala
385 390 395 400
Met Leu Ile Glu Ala Pro
405
<210> 14
<211> 327
<212> PRT
<213> Penicillium chrysogenum
<400> 14
Met Ser Ala Pro Gly Ala Gly His Glu Phe Pro Ala Gln Glu Val Ser
1 5 10 15
Trp Gln Lys Arg Asp Val Leu Leu Phe Ala Asn Ser Ile Gly Val Lys
20 25 30
Ala Asp Glu Leu His Phe Leu Tyr Glu Leu His Pro Asn Phe Ala Val
35 40 45
Phe Pro Thr Tyr Ser Leu Ile Leu Arg Met Ser Lys Asn Thr Ser Asp
50 55 60
Phe Arg Thr Ile Ala Leu Thr Pro Lys Pro Leu Ala Phe Lys Leu Thr
65 70 75 80
Asp Gln Glu Val Thr Asp Phe Tyr Ala Arg Gln Lys Ala Val His Ile
85 90 95
Pro Gly Val Pro Asp Leu Asp His Arg His Gly Val Asp Gly Gln Arg
100 105 110
Lys Ile Thr Ile Leu Lys Pro Leu Pro Thr Thr Ser Ala Gly Arg Lys
115 120 125
Phe Glu Leu Arg Asn Lys Val Ile Gly Val Tyr Asp Lys Gly Lys Pro
130 135 140
Gly Thr Val Ile Glu Thr Glu Gln Ser Ile Val Asp Lys Glu Ser Gly
145 150 155 160
Glu Val Tyr Ser Lys Val Val Ser Ser Gly Phe Leu Val Gly Gln Gly
165 170 175
Gly Trp Gly Gly Pro Lys Gly Pro Ser Thr Val Asn Tyr Ala Pro Pro
180 185 190
Glu Gly Arg Ala Pro Asp Ala Thr His Val Val Gln Ser Asn Ser Glu
195 200 205
Thr Ala His Leu Tyr Arg Leu Asn Gly Asp Tyr Asn Pro Leu His Ala
210 215 220
Thr Pro Glu Pro Gly Gln Lys Met Gly Phe Gly Gly Ile Ile Ile His
225 230 235 240
Gly Leu Phe Ser Trp Asn Ser Ala Ala His Gly Ile Leu Arg Glu Phe
245 250 255
Gly Gly Ser Asn Pro Ala Asn Met Lys Glu Phe Gln Ala Arg Phe Ala
260 265 270
Ser Pro Val Arg Pro Gly Asp Lys Leu Thr Thr Glu Ile Trp Arg Met
275 280 285
Gly Asn Ile Gln Asp Gly Tyr Glu Glu Ile Arg Phe Val Thr Lys Asn
290 295 300
Asp Lys Gly Arg Val Val Leu Ser Asn Gly Arg Cys Leu Leu Lys Val
305 310 315 320
Thr Gly Val Lys Ser Lys Leu
325
<210> 15
<211> 901
<212> PRT
<213> Penicillium chrysogenum
<400> 15
Met Ser Glu Leu Arg Phe Asp Asn Gln Thr Val Val Val Thr Gly Ala
1 5 10 15
Gly Gly Gly Leu Gly Lys Ala Tyr Ala Leu Phe Phe Ala Ser Arg Gly
20 25 30
Ala Asn Val Val Val Asn Asp Leu Gly Ala Ser His Lys Gly Glu Gly
35 40 45
Lys Ser Gly Lys Ala Ala Asp Val Val Val Glu Glu Ile Arg Ala Ala
50 55 60
Gly Gly Lys Ala Val Ala Asn Tyr Asp Ser Val Glu Asn Gly Asp Ala
65 70 75 80
Ile Ile Glu Thr Ala Ile Lys Ala Phe Gly Arg Ile Asp Ile Leu Leu
85 90 95
Asn Asn Ala Gly Ile Leu Arg Asp Ile Ser Phe Lys Asn Met Lys Asp
100 105 110
Ala Asp Trp Asp Leu Ile Asn Arg Val His Thr Tyr Gly Ala Tyr Lys
115 120 125
Cys Ala Arg Ala Ala Trp Pro His Phe Arg Lys Gln Lys Phe Gly Arg
130 135 140
Val Ile Asn Thr Ala Ser Ala Ala Gly Leu Phe Gly Ser Phe Gly Gln
145 150 155 160
Ala Asn Tyr Ser Ala Ala Lys Leu Gly Gln Val Gly Phe Thr Glu Thr
165 170 175
Leu Ala Lys Glu Gly Ala Lys Tyr Asn Ile Ile Ala Asn Val Ile Ala
180 185 190
Pro Ile Ala Ala Ser Arg Met Thr Ala Thr Val Met Pro Pro Asp Val
195 200 205
Leu Glu Asn Leu Lys Pro Asp Trp Val Val Pro Leu Val Ala Ala Leu
210 215 220
Val His Ser Ser Asn Thr Thr Glu Thr Gly Gly Ile Tyr Glu Val Gly
225 230 235 240
Gly Gly His Val Ala Lys Leu Arg Trp Glu Arg Ala Lys Gly Ala Leu
245 250 255
Leu Lys Thr Asp Asp Ser Leu Thr Pro Gly Ala Ile Ala Arg Lys Trp
260 265 270
Asn Asp Val Asn Asp Phe Ser Gln Pro Glu Tyr Pro Thr Gly Pro Ala
275 280 285
Asp Phe Met Ala Phe Leu Glu Asp Gly Leu Lys Thr Pro Ser Ala Gln
290 295 300
Pro Gly Gln Glu Pro Asp Phe Lys Gly Arg Val Ala Leu Val Thr Gly
305 310 315 320
Gly Gly Ala Gly Leu Gly Arg Ala Tyr Cys Leu Gln Phe Ala Lys Leu
325 330 335
Gly Ala Ser Val Val Val Asn Asp Leu Val Asp Pro Glu Pro Val Val
340 345 350
Gln Glu Ile Lys Lys Leu Gly Gly Lys Ala Val Gly Asn Lys Ala Ser
355 360 365
Cys Glu Asp Gly Asp Ala Val Val Lys Ser Ala Ile Asp Ala Phe Gly
370 375 380
Arg Ile Asp Ile Leu Val Asn Asn Ala Gly Ile Leu Arg Asp Lys Ala
385 390 395 400
Phe Thr Asn Met Asp Asp Asn Leu Trp Asn Ser Val Val Asn Val His
405 410 415
Leu Arg Gly Thr Tyr Lys Val Thr Lys Ala Ala Trp Pro Tyr Phe Leu
420 425 430
Lys Gln Lys Tyr Gly Arg Val Val Asn Thr Ala Ser Thr Ser Gly Ile
435 440 445
Tyr Gly Asn Phe Gly Gln Ala Asn Tyr Ala Ala Ala Lys Leu Gly Ile
450 455 460
Leu Gly Leu Ser Arg Thr Leu Ala Leu Glu Gly Ala Lys Tyr Asn Ile
465 470 475 480
Lys Val Asn Thr Ile Ala Pro Asn Ala Gly Thr Asn Met Thr Arg Thr
485 490 495
Ile Met Pro Glu Glu Met Val Gln Ala Phe Lys Pro Asp Tyr Val Ala
500 505 510
Pro Leu Val Val Leu Leu Cys Ser Asp Met Ala Pro Glu Pro Ser Thr
515 520 525
Lys Gly Leu Phe Glu Cys Gly Ser Gly Trp Phe Gly Arg Thr Arg Trp
530 535 540
Gln Arg Thr Gly Gly His Gly Phe Pro Val Asp Val Lys Leu Thr Pro
545 550 555 560
Glu Glu Val Val Arg Asn Trp Lys Gln Ile Ile Asn Phe Asp Asp Gly
565 570 575
Arg Ala Asp His Pro Glu Asp Gly Gln Ala Gly Ala Glu Lys Ile Met
580 585 590
Ala Asn Met Ser Asn Arg Val His Gly Asp Thr Ser Thr Glu Asn Glu
595 600 605
Thr Leu Lys Asn Ile Lys Lys Ala Lys Ala Leu Ser Ser Glu Gly Thr
610 615 620
Pro Phe Asn Tyr Glu Asp Arg Asp Val Ile Leu Tyr Asn Leu Ser Leu
625 630 635 640
Gly Ala Lys Arg Thr Asp Leu Pro Leu Val Tyr Glu Asn Asn Asp Gln
645 650 655
Phe Gln Ala Leu Pro Ser Tyr Gly Val Val Pro Trp Phe Asn Thr Ala
660 665 670
Thr Pro Trp Asn Met Asp Asp Leu Val Lys Asp Phe Ser Pro Met Met
675 680 685
Leu Leu His Gly Glu Gln Tyr Met Glu Val Arg Lys Phe Pro Ile Pro
690 695 700
Thr Thr Ala Asn Thr Leu Thr Tyr Pro Lys Leu Ile Asp Val Ile Asp
705 710 715 720
Lys Gly Asn Ala Ala Ile Val Val Ala Gly Tyr Thr Thr Lys Asp Ala
725 730 735
Lys Thr Gly Glu Asp Leu Phe Tyr Asn Glu Ser Ser Val Phe Ile Arg
740 745 750
Gly Ser Gly Gly Phe Gly Gly Ser Pro Lys Pro Thr Ala Val Arg Pro
755 760 765
Lys Ala Ala Thr Ala Ala Tyr Lys Ala Pro Gln Arg Gln Pro Asp Ala
770 775 780
Val Val Glu Glu Lys Thr Ser Glu Asp Gln Ala Ala Leu Tyr Arg Leu
785 790 795 800
Asn Gly Asp Arg Asn Pro Leu His Ile Asp Pro Glu Phe Ser Lys Val
805 810 815
Gly Gly Phe Lys Thr Pro Ile Leu His Gly Leu Cys Ser Leu Gly Val
820 825 830
Ser Ala Lys Ala Val Phe Ser Lys Tyr Gly Pro Tyr Lys Asn Leu Lys
835 840 845
Val Arg Phe Ala Gly Val Val Leu Pro Gly Gln Thr Leu Lys Thr Glu
850 855 860
Met Trp Lys Glu Gly Asn Thr Val Leu Phe Gln Ala Thr Val Val Glu
865 870 875 880
Thr Gly Lys Pro Ala Ile Thr Gly Ala Gly Ala Glu Leu Leu Glu Gly
885 890 895
Ala Lys Ala Lys Leu
900
<210> 16
<211> 319
<212> PRT
<213> Penicillium chrysogenum
<400> 16
Met Leu Asn Ser Thr Leu Arg Phe Val Ser Leu Arg Arg Gly Pro Thr
1 5 10 15
Val Ala Arg Asn Phe Cys Ser Thr Ser Ala Val Arg Ala Ala Glu Val
20 25 30
Lys Ser Leu Gly Val Val Gly Ala Gly Gln Met Gly Leu Gly Ile Ala
35 40 45
Leu Val Ala Ala Gln Lys Ala Asn Val Pro Val Thr Leu Val Asp Thr
50 55 60
Ser Gln Ala Ser Leu Asp Lys Gly Leu Lys Phe Ala Asp Lys Leu Leu
65 70 75 80
Glu Lys Asp Val Ala Lys Gln Arg Leu Thr Arg Glu Ala Ala Asp Glu
85 90 95
Ala Arg Gly Leu Ile Ser Thr Ser Leu Thr Leu Asp Gly Leu Ser Ala
100 105 110
Val Asp Phe Val Ile Glu Ala Val Pro Glu Ile Pro Asp Leu Lys Thr
115 120 125
Lys Ile Phe Ala Ser Leu Ala Gln Ile Ala Pro Lys His Ala Ile Leu
130 135 140
Ala Thr Asn Thr Ser Ser Ile Ser Ile Thr Lys Ile Ala Ala Ala Thr
145 150 155 160
Ser Ala Asp Pro Thr Asp Leu Gln Ala Ser Ser Arg Val Ile Ser Thr
165 170 175
His Phe Met Asn Pro Val Pro Ile Gln Lys Gly Val Glu Ile Ile Arg
180 185 190
Gly Leu Gln Thr Ser Glu Glu Thr Met Asp Thr Ala Ile Ala Phe Val
195 200 205
Gln Arg Met Gly Lys Val Ala Ser Val Ser Ala Asp Thr Pro Gly Phe
210 215 220
Leu Ala Asn Arg Ile Leu Met Pro Tyr Ile Asn Glu Ala Val Ile Cys
225 230 235 240
Leu Glu Thr Gly Val Gly Gln Arg Glu Asp Ile Asp Asn Ile Met Lys
245 250 255
Thr Gly Thr Asn Val Pro Met Gly Pro Leu Val Leu Ala Asp Phe Ile
260 265 270
Gly Leu Asp Thr Cys Leu Ala Ile Met Asn Val Leu His Gln Asp Thr
275 280 285
Gly Asp Ser Lys Tyr Arg Pro Ala Gly Leu Leu Arg Arg Met Val Asp
290 295 300
Ala Gly Trp Leu Gly Lys Lys Ser Gly Lys Gly Phe Tyr Asp Tyr
305 310 315
<210> 17
<211> 335
<212> PRT
<213> Penicillium chrysogenum
<400> 17
Met Ala Thr Glu Tyr Pro His Arg Ile Ala Leu Leu Gly Leu Gly Thr
1 5 10 15
Ile Gly Leu Ser Met Leu Ala Met His Leu Arg Arg Pro Asp Thr Ser
20 25 30
Ile Thr Val Tyr Asp Pro Arg Pro Asn Tyr Glu Ser Gln Ile Arg Ser
35 40 45
Thr Leu Pro Ser Leu Leu Asp Cys Pro Glu Ser Thr Thr Leu Ile Asp
50 55 60
Asp Leu Leu Arg Thr Ser Arg Leu Lys Leu Ala Ser Thr Val Ala Glu
65 70 75 80
Ala Ile Gln Asn Ala Asn Ile Ile Gln Glu Gln Ser Pro Glu Val Thr
85 90 95
Ala Ser Lys Gln Ala Leu Trp Lys Glu Val Ala Gly Leu Thr Gly Pro
100 105 110
Asp Val His Leu Trp Ser Ser Ser Ser Gly Ile Ser Ala Ser Val Gln
115 120 125
Ala Ser Gly Cys Glu Ala Ala Asp Arg Val Ala Glu Arg Leu Leu Val
130 135 140
Ala His Pro Phe Asn Pro Pro His Leu Met Pro Leu Ile Glu Ile Val
145 150 155 160
Pro Gly Pro Glu Thr Asn Pro Glu Arg Val Glu Phe Val Arg Lys Tyr
165 170 175
Phe Gly Asp Val Pro Gly Pro Arg Ala Ser Gly Gly Asp Gln Ser Ala
180 185 190
Ser Gln His Tyr Arg Pro Ile Thr Leu His Lys Glu Ile Pro Gly Phe
195 200 205
Val Gly Asn Arg Leu Ala Phe Ala Leu Leu Arg Glu Ala Cys Tyr Leu
210 215 220
Val Gly Glu Gly Val Val Ser Ala Lys Asp Leu Asp Ser Leu Val Thr
225 230 235 240
Ala Ser Leu Gly Pro Arg Trp Ala Gly Ser Gly Val Phe Glu Ser Tyr
245 250 255
His Ala Gly Gly Gly Glu Gly Gly Ile Gly Ala Phe Leu Gln Lys Leu
260 265 270
Thr Pro Thr Ile Gln Asp Val Trp Gly Glu Leu Gly Gln Ile Asp Ile
275 280 285
Gln Gly Glu Gln Thr Trp Lys Asp Val Val Val Lys Gln Thr Glu Asp
290 295 300
Ala Tyr Gly Pro Tyr Thr Pro Glu Thr Arg Lys Lys Lys Glu Glu Met
305 310 315 320
Leu Arg Asp Val Val Glu Leu Gln Lys Lys Lys Trp Gly Glu Leu
325 330 335
<210> 18
<211> 505
<212> PRT
<213> Acinetobacter sp.
<400> 18
Met Thr His Pro Ile Lys Lys Ile Ala Ile Ile Gly Val Gly Val Met
1 5 10 15
Gly Ser Gly Ile Ala Gln Ile Ala Ala Gln Ser Gly His Ile Thr Tyr
20 25 30
Leu Tyr Asp Ala Lys Ala Gly Ala Ala Gln Gln Ala Lys Gln Gln Leu
35 40 45
Ala Ile Thr Phe Gln Lys Leu Leu Asp Lys Asn Lys Ile Thr Thr Glu
50 55 60
Tyr Ala Asp Ala Ala Asn Ala Asn Leu Leu Ile Ala Asn Glu Leu His
65 70 75 80
Asp Leu Lys Asp Cys Asp Leu Ile Val Glu Ala Ile Val Glu Arg Leu
85 90 95
Asp Ile Lys Gln Ser Leu Met Ser Gln Leu Glu Ala Ile Val Pro Glu
100 105 110
Thr Thr Ile Leu Ala Ser Asn Thr Ser Ser Leu Ser Ile Thr Ala Ile
115 120 125
Ala Ser Asn Cys Lys His Pro Glu Arg Val Ala Gly Tyr His Phe Phe
130 135 140
Asn Pro Val Pro Leu Met Lys Val Val Glu Val Ile Gln Gly Leu Lys
145 150 155 160
Thr Asp Pro Lys His Ile Glu Thr Leu Asn Gln Leu Ser Arg Val Leu
165 170 175
Gly His Arg Pro Val Val Ala Lys Asp Thr Pro Gly Phe Ile Ile Asn
180 185 190
His Ala Gly Arg Ala Tyr Gly Thr Glu Ala Leu Lys Ile Leu Asn Glu
195 200 205
Asn Val Thr Asp Ile Ser Glu Ile Asp Arg Ile Leu Arg Asp Gly Val
210 215 220
Gly Phe Arg Met Gly Pro Phe Glu Leu Met Asp Leu Thr Gly Leu Asp
225 230 235 240
Val Ser His Pro Val Met Glu Ser Ile Tyr His Gln Tyr Tyr Glu Glu
245 250 255
Ala Arg Tyr Arg Pro Asn Ser Leu Thr Lys Gln Met Leu Glu Ala Lys
260 265 270
Gln Leu Gly Arg Lys Val Gly Gln Gly Phe Tyr Asp Tyr Arg Thr Gly
275 280 285
Ser Lys Thr Gly Glu Thr Ser Ala Lys Val Ala Glu Arg Leu Thr Leu
290 295 300
Tyr Pro Lys Val Trp Ile Ala Ala Asp Phe Glu Asp Asp Lys Gln Leu
305 310 315 320
Leu Ile Asn Tyr Leu Thr Thr His Asn Ile Gln Leu Asp Val Gly Ala
325 330 335
Lys Pro Gln Ala Asp Ser Leu Cys Leu Leu Ala Cys Tyr Gly Glu Asp
340 345 350
Thr Thr His Ala Ala Leu Arg Leu Asn Val Asn Pro Ala His Ser Val
355 360 365
Ala Ile Asp Met Leu Tyr Gly Ile Glu Lys His Arg Thr Leu Met Pro
370 375 380
Ser Leu Ile Thr Glu Val Thr Tyr Ser His Ala Ala His Ser Ile Phe
385 390 395 400
Asn Leu Asp Gly Ala Met Val Ser Thr Ile Gly Glu Ser Ile Gly Phe
405 410 415
Val Ala Gln Arg Ile Leu Ala Met Val Ile Asn Leu Gly Cys Asp Ile
420 425 430
Ala Gln Gln Ala Ile Ala Ser Val Asp Asp Ile Asn Ala Ala Val Arg
435 440 445
Leu Gly Leu Gly Tyr Pro Phe Gly Pro Ile Glu Trp Gly Asp Glu Ile
450 455 460
Gly Ser Asn Lys Ile Leu Leu Ile Leu Asn Arg Ile Thr Ala Leu Thr
465 470 475 480
Ser Asp Pro Arg Tyr Arg Pro Ser Pro Trp Leu Gln Arg Arg Val Ala
485 490 495
Leu Asn Leu Pro Leu Thr Phe Thr Thr
500 505
<210> 19
<211> 282
<212> PRT
<213> Clostridium kluyveri
<400> 19
Met Ser Ile Lys Ser Val Ala Val Leu Gly Ser Gly Thr Met Ser Arg
1 5 10 15
Gly Ile Val Gln Ala Phe Ala Glu Ala Gly Ile Asp Val Ile Ile Arg
20 25 30
Gly Arg Thr Glu Gly Ser Ile Gly Lys Gly Leu Ala Ala Val Lys Lys
35 40 45
Ala Tyr Asp Lys Lys Val Ser Lys Gly Lys Ile Ser Gln Glu Asp Ala
50 55 60
Asp Lys Ile Val Gly Arg Val Ser Thr Thr Thr Glu Leu Glu Lys Leu
65 70 75 80
Ala Asp Cys Asp Leu Ile Ile Glu Ala Ala Ser Glu Asp Met Asn Ile
85 90 95
Lys Lys Asp Tyr Phe Gly Lys Leu Glu Glu Ile Cys Lys Pro Glu Thr
100 105 110
Ile Phe Ala Thr Asn Thr Ser Ser Leu Ser Ile Thr Glu Val Ala Thr
115 120 125
Ala Thr Lys Arg Pro Asp Lys Phe Ile Gly Met His Phe Phe Asn Pro
130 135 140
Ala Asn Val Met Lys Leu Val Glu Ile Ile Arg Gly Met Asn Thr Ser
145 150 155 160
Gln Glu Thr Phe Asp Ile Ile Lys Glu Ala Ser Ile Lys Ile Gly Lys
165 170 175
Thr Pro Val Glu Val Ala Glu Ala Pro Gly Phe Val Val Asn Lys Ile
180 185 190
Leu Val Pro Met Ile Asn Glu Ala Val Gly Ile Leu Ala Glu Gly Ile
195 200 205
Ala Ser Ala Glu Asp Ile Asp Thr Ala Met Lys Leu Gly Ala Asn His
210 215 220
Pro Met Gly Pro Leu Ala Leu Gly Asp Leu Ile Gly Leu Asp Val Val
225 230 235 240
Leu Ala Val Met Asp Val Leu Tyr Ser Glu Thr Gly Asp Ser Lys Tyr
245 250 255
Arg Ala His Thr Leu Leu Arg Lys Tyr Val Arg Ala Gly Trp Leu Gly
260 265 270
Arg Lys Ser Gly Lys Gly Phe Phe Ala Tyr
275 280
<210> 20
<211> 319
<212> PRT
<213> Clostridium kluyveri
<400> 20
Met Asp Ile Lys Asn Val Ala Val Leu Gly Thr Gly Thr Met Gly Asn
1 5 10 15
Gly Ile Val Gln Leu Cys Ala Glu Ser Gly Leu Asn Val Asn Met Phe
20 25 30
Gly Arg Thr Asp Ala Ser Leu Glu Arg Gly Phe Thr Ser Ile Lys Thr
35 40 45
Ser Leu Lys Asn Leu Glu Glu Lys Gly Lys Ile Lys Thr Asn Ile Ser
50 55 60
Lys Glu Ile Leu Lys Arg Ile Lys Gly Val Lys Thr Ile Glu Glu Ala
65 70 75 80
Val Glu Gly Val Asp Phe Val Ile Glu Cys Ile Ala Glu Asp Leu Glu
85 90 95
Leu Lys Gln Glu Val Phe Ser Lys Leu Asp Glu Ile Cys Ala Pro Glu
100 105 110
Val Ile Leu Ala Ser Asn Thr Ser Gly Leu Ser Pro Thr Asp Ile Ala
115 120 125
Ile Asn Thr Lys His Pro Glu Arg Val Val Ile Ala His Phe Trp Asn
130 135 140
Pro Pro Gln Phe Ile Pro Leu Val Glu Val Val Pro Gly Lys His Thr
145 150 155 160
Asp Ser Lys Thr Val Asp Ile Thr Met Asp Trp Ile Glu His Ile Gly
165 170 175
Lys Lys Gly Val Lys Met Arg Lys Glu Cys Leu Gly Phe Ile Gly Asn
180 185 190
Arg Leu Gln Leu Ala Leu Leu Arg Glu Ala Leu Tyr Ile Val Glu Gln
195 200 205
Gly Phe Ala Thr Ala Glu Glu Val Asp Lys Ala Ile Glu Tyr Gly His
210 215 220
Gly Arg Arg Leu Pro Val Thr Gly Pro Ile Cys Ser Ala Asp Leu Gly
225 230 235 240
Gly Leu Asp Ile Phe Asn Asn Ile Ser Ser Tyr Leu Phe Lys Asp Leu
245 250 255
Cys Asn Asp Thr Glu Pro Ser Lys Leu Leu Lys Ser Lys Val Asp Gly
260 265 270
Gly Asn Leu Gly Ser Lys Thr Gly Lys Gly Phe Tyr Asn Trp Thr Pro
275 280 285
Glu Phe Leu Gln Lys Lys Gln Asn Glu Arg Ile Gln Leu Leu Met Asp
290 295 300
Phe Leu Glu Lys Asp Lys Asn Asp Lys Ser Ile Glu Arg Asn Ile
305 310 315
<210> 21
<211> 475
<212> PRT
<213> Escherichia coli
<400> 21
Met Met Ile Asn Val Gln Thr Val Ala Val Ile Gly Ser Gly Thr Met
1 5 10 15
Gly Ala Gly Ile Ala Glu Val Ala Ala Ser His Gly His Gln Val Leu
20 25 30
Leu Tyr Asp Ile Ser Ala Glu Ala Leu Thr Arg Ala Ile Asp Gly Ile
35 40 45
His Ala Arg Leu Asn Ser Arg Val Thr Arg Gly Lys Leu Thr Ala Glu
50 55 60
Thr Cys Glu Arg Thr Leu Lys Arg Leu Ile Pro Val Thr Asp Ile His
65 70 75 80
Ala Leu Ala Ala Ala Asp Leu Val Ile Glu Ala Ala Ser Glu Arg Leu
85 90 95
Glu Val Lys Lys Ala Leu Phe Ala Gln Leu Ala Glu Val Cys Pro Pro
100 105 110
Gln Thr Leu Leu Thr Thr Asn Thr Ser Ser Ile Ser Ile Thr Ala Ile
115 120 125
Ala Ala Glu Ile Lys Asn Pro Glu Arg Val Ala Gly Leu His Phe Phe
130 135 140
Asn Pro Ala Pro Val Met Lys Leu Val Glu Val Val Ser Gly Leu Ala
145 150 155 160
Thr Ala Ala Glu Val Val Glu Gln Leu Cys Glu Leu Thr Leu Ser Trp
165 170 175
Gly Lys Gln Pro Val Arg Cys His Ser Thr Pro Gly Phe Ile Val Asn
180 185 190
Arg Val Ala Arg Pro Tyr Tyr Ser Glu Ala Trp Arg Ala Leu Glu Glu
195 200 205
Gln Val Ala Ala Pro Glu Val Ile Asp Ala Ala Leu Arg Asp Gly Ala
210 215 220
Gly Phe Pro Met Gly Pro Leu Glu Leu Thr Asp Leu Ile Gly Gln Asp
225 230 235 240
Val Asn Phe Ala Val Thr Cys Ser Val Phe Asn Ala Phe Trp Gln Glu
245 250 255
Arg Arg Phe Leu Pro Ser Leu Val Gln Gln Glu Leu Val Ile Gly Gly
260 265 270
Arg Leu Gly Lys Lys Ser Gly Leu Gly Val Tyr Asp Trp Arg Ala Glu
275 280 285
Arg Glu Ala Val Val Gly Leu Glu Ala Val Ser Asp Ser Phe Ser Pro
290 295 300
Met Lys Val Glu Lys Lys Ser Asp Gly Val Thr Glu Ile Asp Asp Val
305 310 315 320
Leu Leu Ile Glu Thr Gln Gly Glu Thr Ala Gln Ala Leu Ala Ile Arg
325 330 335
Leu Ala Arg Pro Val Val Val Ile Asp Lys Met Ala Gly Lys Val Val
340 345 350
Thr Ile Ala Ala Ala Ala Val Asn Pro Asp Ser Ala Thr Arg Lys Ala
355 360 365
Ile Tyr Tyr Leu Gln Gln Gln Gly Lys Thr Val Leu Gln Ile Ala Asp
370 375 380
Tyr Pro Gly Met Leu Ile Trp Arg Thr Val Ala Met Ile Ile Asn Glu
385 390 395 400
Ala Leu Asp Ala Leu Gln Lys Gly Val Ala Ser Glu Gln Asp Ile Asp
405 410 415
Thr Ala Met Arg Leu Gly Val Asn Tyr Pro Tyr Gly Pro Leu Ala Trp
420 425 430
Gly Ala Gln Leu Gly Trp Gln Arg Ile Leu Arg Leu Leu Glu Asn Leu
435 440 445
Gln His His Tyr Gly Glu Glu Arg Tyr Arg Pro Cys Ser Leu Leu Arg
450 455 460
Gln Arg Ala Leu Leu Glu Ser Gly Tyr Glu Ser
465 470 475
<210> 22
<211> 320
<212> PRT
<213> Euglena gracilis
<400> 22
Met Met Ser Arg Asn Val Phe Thr Cys Cys Arg Ala Ile Arg Thr Tyr
1 5 10 15
Ala Thr Ala Pro Gly Leu Ser Thr Val Gly Val Val Gly Met Gly Ala
20 25 30
Met Gly His Gly Ile Ala Gln Met Thr Ala Ala Ala Gly Tyr Lys Val
35 40 45
Val Ala Val Asp Ile Asp Ala Asn Met Leu Ser Lys Gly Ile Lys Ala
50 55 60
Val Glu Asp Ser Leu Ser Lys Val Ala Ala Lys Ala Val Lys Asp Gly
65 70 75 80
Lys Ala Asp Lys Ala Thr Ala Glu Lys Asn Ala Ala Asp Val Arg Ser
85 90 95
Arg Ile Thr Thr Ser Gly Asp Ile Gly Ala Leu Ser Ser Cys Asp Leu
100 105 110
Val Ile Glu Ser Ile Ile Glu Asp Leu Asn Ile Lys Lys Lys Phe Phe
115 120 125
Ala Asp Leu Gly Lys Val Ala Gly Ala Asn Ala Ile Leu Ala Ser Asn
130 135 140
Thr Ser Ser Phe Pro Ile Thr Gln Leu Gly Glu Ala Ser Gly Arg Thr
145 150 155 160
Ser Asn Phe Leu Gly Leu His Phe Phe Asn Pro Val Gln Met Met Lys
165 170 175
Leu Val Glu Val Ile Lys Thr Lys Asp Thr Lys Glu Asp Val Tyr Lys
180 185 190
Leu Gly Phe Ala Phe Ser Lys Ser Ile Gly Lys Glu Pro Val Ala Cys
195 200 205
Gly Asp Thr Pro Gly Phe Ile Val Asn Arg Leu Leu Val Pro Phe Leu
210 215 220
Ala Gln Gly Leu Leu Met Leu Asp Arg Gly Val Ala Ser Val Gln Asp
225 230 235 240
Ile Asp Val Ala Met Met Tyr Gly Ala Gly Met Pro Met Gly Pro Leu
245 250 255
Thr Leu Ala Asp Tyr Val Gly Leu Asp Val Cys Met His Ile Leu Glu
260 265 270
Gly Trp Thr Ser Gln Tyr Pro Asn Glu Pro Ala Phe Val Ile Pro Gln
275 280 285
Pro Leu Lys Ala Lys Val Ala Ala Gly Lys Leu Gly Arg Lys Thr Gly
290 295 300
Glu Gly Phe Trp Lys Trp Glu Gly Asp Lys Pro Val Ala Pro Ala Ala
305 310 315 320
<210> 23
<211> 505
<212> PRT
<213> Pseudomonas putida
<400> 23
Met Ala Ala Leu Ala Ser Asn Val Gln Val Ala Val Ile Gly Ala Gly
1 5 10 15
Ala Met Gly Ala Gly Ile Ala Gln Val Ala Ala Gln Ala Gly His Pro
20 25 30
Val Lys Leu Tyr Asp Asn Arg Pro Gly Ala Ala Ala Gln Ala Val Thr
35 40 45
Gly Ile Asp Arg Gln Leu Ala Arg Leu Val Asp Lys Gly Lys Leu Leu
50 55 60
Ala Ala Glu Arg Glu Thr Ile Asn Ala Arg Leu Cys Pro Val Asp Thr
65 70 75 80
Leu Glu Ala Leu Ala Asp Ala Gly Leu Val Ile Glu Ala Ile Val Glu
85 90 95
Asn Leu Gln Val Lys Gln Ala Leu Phe Ser Gln Leu Glu Thr Leu Cys
100 105 110
Ala Ala Asp Cys Ile Leu Ala Ser Asn Thr Ser Ser Leu Ser Ile Thr
115 120 125
Ser Leu Ala Ala Gly Leu Glu Arg Pro Gln His Val Val Gly Met His
130 135 140
Phe Phe Asn Pro Ala Pro Leu Met Ala Leu Val Glu Val Val Ser Gly
145 150 155 160
Leu Ala Thr Asp Pro Ala Val Ala Ala Cys Ile Tyr Ala Thr Ala Gln
165 170 175
Ala Trp Gly Lys Gln Pro Val His Ala Arg Ser Thr Pro Gly Phe Ile
180 185 190
Val Asn Arg Val Ala Arg Pro Phe Tyr Ala Glu Ser Leu Arg Leu Leu
195 200 205
Gln Glu Gly Ala Ala Asp Cys Ala Ser Leu Asp Ala Leu Met Arg Asp
210 215 220
Ser Gly Gly Phe Arg Met Gly Ala Phe Glu Leu Thr Asp Leu Ile Gly
225 230 235 240
His Asp Val Asn Tyr Ala Val Thr Cys Ser Val Phe Asp Ala Phe Tyr
245 250 255
Gly Asp Phe Arg Phe Gln Pro Ser Leu Val Gln Lys Glu Leu Val Asp
260 265 270
Ala Gly His Leu Gly Arg Lys Thr Gly Gln Gly Phe Tyr Arg Tyr Ala
275 280 285
Glu Gly Val Glu Arg Pro Gln Pro Ala Glu Leu His Ser Ser Ala Cys
290 295 300
Ala Glu Ala Cys Val Val Glu Gly Asn Leu Gly Val Met Gln Pro Leu
305 310 315 320
Val Glu Arg Leu Arg Gln Ser Gly Ile Ala Val Thr Gln Arg Ala Gly
325 330 335
Ser Gly Leu Ile Gln Val Gly Asp Ala Thr Leu Ala Leu Ser Asp Gly
340 345 350
Arg Leu Ala Ser Gln Arg Ala Arg Glu Asp Gly Leu Arg Asn Leu Val
355 360 365
Leu Leu Asp Leu Ala Leu Asp Tyr Ser Ser Ala Thr Arg Ile Ala Ile
370 375 380
Ser Trp Ser Ala Asp Thr Ser Asp Ser Ala Arg Asp Gln Ala Val Ala
385 390 395 400
Leu Leu Gln Arg Ala Gly Leu Lys Val Thr Gly Val Ala Asp Leu Pro
405 410 415
Gly Leu Val Val Leu Arg Thr Val Ala Met Leu Ala Asn Glu Ala Ala
420 425 430
Asp Ala Val Leu Gln Gly Val Gly Ser Ala Ala Asp Ile Asp Leu Ala
435 440 445
Met Arg Ala Gly Val Asn Tyr Pro Cys Gly Pro Leu Ala Trp Ala Ala
450 455 460
Asn Ile Gly Ile Ala His Thr Leu Arg Val Leu Asp Asn Leu Gln Cys
465 470 475 480
Ser Tyr Gly Glu Ser Arg Tyr Arg Pro Ser Leu Leu Leu Arg Arg Cys
485 490 495
Glu Ala Lys Gly Gly Thr Leu His Asp
500 505
<210> 24
<211> 246
<212> PRT
<213> Ralstonia eutropha
<400> 24
Met Thr Gln Arg Ile Ala Tyr Val Thr Gly Gly Met Gly Gly Ile Gly
1 5 10 15
Thr Ala Ile Cys Gln Arg Leu Ala Lys Asp Gly Phe Arg Val Val Ala
20 25 30
Gly Cys Gly Pro Asn Ser Pro Arg Arg Glu Lys Trp Leu Glu Gln Gln
35 40 45
Lys Ala Leu Gly Phe Asp Phe Ile Ala Ser Glu Gly Asn Val Ala Asp
50 55 60
Trp Asp Ser Thr Lys Thr Ala Phe Asp Lys Val Lys Ser Glu Val Gly
65 70 75 80
Glu Val Asp Val Leu Ile Asn Asn Ala Gly Ile Thr Arg Asp Val Val
85 90 95
Phe Arg Lys Met Thr Arg Ala Asp Trp Asp Ala Val Ile Asp Thr Asn
100 105 110
Leu Thr Ser Leu Phe Asn Val Thr Lys Gln Val Ile Asp Gly Met Ala
115 120 125
Asp Arg Gly Trp Gly Arg Ile Val Asn Ile Ser Ser Val Asn Gly Gln
130 135 140
Lys Gly Gln Phe Gly Gln Thr Asn Tyr Ser Thr Ala Lys Ala Gly Leu
145 150 155 160
His Gly Phe Thr Met Ala Leu Ala Gln Glu Val Ala Thr Lys Gly Val
165 170 175
Thr Val Asn Thr Val Ser Pro Gly Tyr Ile Ala Thr Asp Met Val Lys
180 185 190
Ala Ile Arg Gln Asp Val Leu Asp Lys Ile Val Ala Thr Ile Pro Val
195 200 205
Lys Arg Leu Gly Leu Pro Glu Glu Ile Ala Ser Ile Cys Ala Trp Leu
210 215 220
Ser Ser Glu Glu Ser Gly Phe Ser Thr Gly Ala Asp Phe Ser Leu Asn
225 230 235 240
Gly Gly Leu His Met Gly
245
<210> 25
<211> 284
<212> PRT
<213> Rhodococcus sp.
<400> 25
Met Thr Ser Thr Ala Pro Lys Asn Val Gly Val Val Gly Gly Gly Arg
1 5 10 15
Met Gly Ala Gly Ile Ala Gln Val Phe Ala Thr Leu Gly Ser Thr Val
20 25 30
Ile Ile Ala Glu Ser Gly Asp Arg Glu Ala Ala Val Lys Arg Val Ser
35 40 45
Asp Gly Leu Asp Arg Ala His Glu Arg Gly Lys Leu Gly Asp Val Asp
50 55 60
Pro Ala Thr Ile Leu Gly Arg Val Ser Thr Val Ala Ala Pro Asp Ala
65 70 75 80
Leu Pro Pro Ala Leu Asp Leu Val Val Glu Ala Val Pro Glu Leu Val
85 90 95
Asp Leu Lys Leu Ser Val Leu Ser Leu Val Glu Lys Thr Val Ser Pro
100 105 110
Thr Thr Val Ile Ala Ser Asn Thr Ser Ser Ile Ser Ile Ala Glu Leu
115 120 125
Gly Ser Ala Leu Gly Asp Pro Ala Arg Leu Ile Gly Met His Phe Phe
130 135 140
Asn Pro Val Pro Ala Ser Ser Leu Val Glu Ile Val Arg Ala Pro Ala
145 150 155 160
Thr Asp Ala Gly Val Val Glu Lys Val Arg Glu Trp Val Ala Gln Leu
165 170 175
Gly Lys Thr Glu Val Leu Val Asn Asp Ser Pro Gly Phe Ala Thr Ser
180 185 190
Arg Leu Gly Val Cys Leu Gly Leu Glu Ala Ile Arg Met Leu Glu Glu
195 200 205
Gly Val Ala Asp Ala Glu Ser Ile Asp Arg Ala Met Glu Leu Gly Tyr
210 215 220
Arg His Pro Met Gly Pro Leu Arg Ser Thr Asp Leu Val Gly Leu Asp
225 230 235 240
Val Arg Leu Ala Ile Ala Glu His Leu Ala Lys Thr Leu Gly Asp Arg
245 250 255
Phe Ala Pro Pro Ala Leu Leu Arg Glu Lys Val Ala Lys Gly Glu Leu
260 265 270
Gly Arg Lys Thr Gly Gln Gly Phe Phe Thr Trp Ser
275 280
<210> 26
<211> 699
<212> PRT
<213> Rhodopseudomonas palustris
<400> 26
Met Ser Glu Val Val Thr Arg Ala Thr Gln Asp Gln Val Ala Ile Val
1 5 10 15
Thr Val Asp Ser Pro Pro Val Asn Ala Leu Ser Ala Ala Val Arg Arg
20 25 30
Gly Ile Leu Glu Asn Val Asn Ala Ala Val Ala Asp Pro Ala Val Gln
35 40 45
Ala Ile Val Leu Val Cys Ala Gly Arg Thr Phe Ile Ala Gly Ala Asp
50 55 60
Ile Thr Glu Phe Gly Lys Pro Pro Gln Pro Pro Ala Leu Asn Asp Val
65 70 75 80
Ile Ala Ala Leu Glu Asn Ser Pro Lys Pro Thr Ile Ala Ala Ile His
85 90 95
Gly Thr Ala Leu Gly Gly Gly Leu Glu Val Ala Leu Gly Cys His Phe
100 105 110
Arg Val Ala Val Lys Glu Ala Lys Leu Gly Leu Pro Glu Val Lys Leu
115 120 125
Gly Leu Leu Pro Gly Ala Gly Gly Thr Gln Arg Leu Pro Arg Ala Val
130 135 140
Gly Pro Glu Leu Ala Val Gln Met Ile Val Gly Gly Ser Pro Ile Gly
145 150 155 160
Ala Ala Glu Ala Leu Lys His Gly Leu Val Glu Glu Val Val Glu Asn
165 170 175
Leu Val Ala Gly Ala Val Ala Phe Ala Lys Lys Val Leu Ala Glu Lys
180 185 190
Arg Pro Leu Arg Arg Leu Arg Asp Asp Asp Ser Lys Leu Ala Ala Ala
195 200 205
Lys Ala Asp Arg Ser Ile Phe Thr Asn Ala Val Ala Ala Met Thr Lys
210 215 220
Lys Ala Arg Gly Leu Glu Ala Pro Phe Ala Cys Ala Asp Ala Ile Gly
225 230 235 240
Ala Ala Ile Asp Leu Pro Phe Glu Glu Gly Leu Lys Lys Glu Arg Glu
245 250 255
Gly Phe Met Lys Leu Val Val Ser Asp Gln Ser Lys Ala Gln Arg Tyr
260 265 270
Ala Phe Phe Ala Glu Arg Glu Ala Ala Lys Val Asp Gly Val Pro Asp
275 280 285
Gly Thr Lys Pro Arg Pro Val Ser Arg Val Ala Ile Ile Gly Ala Gly
290 295 300
Thr Met Gly Gly Gly Ile Ala Met Ser Phe Ala Asn Ala Gly Ile Pro
305 310 315 320
Val Thr Leu Ile Glu Thr Gly Glu Glu Gln Leu Lys Arg Gly Leu Gly
325 330 335
Ile Met Gln Lys Asn Trp Glu Ala Thr Ala Ala Arg Gly Gly Leu Pro
340 345 350
Pro Asp Ala Pro Ala Lys Arg Met Ala Leu Ile Thr Gly Leu Val Gly
355 360 365
Leu Glu Asn Val Lys Asp Ala Asp Leu Ile Ile Glu Ala Val Phe Glu
370 375 380
Thr Met Ala Val Lys Lys Glu Val Phe Thr Ala Val Asp Ala His Ala
385 390 395 400
Lys Pro Gly Ala Val Leu Ala Ser Asn Thr Ser Tyr Leu Ser Ile Asp
405 410 415
Glu Ile Ala Ala Thr Thr Lys Arg Pro Gln Asp Val Leu Gly Met His
420 425 430
Phe Phe Ser Pro Ala Asn Val Met Lys Leu Cys Glu Ile Val Arg Gly
435 440 445
Ala Lys Thr Ala Pro Asp Ala Leu Leu Thr Ala Val Ser Ile Ala Lys
450 455 460
Lys Ile Ala Lys Val Pro Val Val Val Gly Val Cys Asp Gly Phe Val
465 470 475 480
Gly Asn Arg Met Leu Ala Ala Arg Ser Lys Gln Ser Glu Lys Leu Leu
485 490 495
Phe Glu Gly Ala Leu Pro Gln Gln Val Asp Ala Val Val Thr Lys Phe
500 505 510
Gly Met Pro Met Gly Pro Phe Ala Met Gly Asp Leu Ala Gly Leu Asp
515 520 525
Ile Gly Trp Arg Ser Arg Lys Asp Arg Gly Ile Lys Ser Glu Ile Ala
530 535 540
Asp Ala Leu Cys Glu Ala Gly Arg Phe Gly Gln Lys Thr Gly Lys Gly
545 550 555 560
Tyr Tyr Lys Tyr Glu Gln Gly Ser Arg Ala Pro Met Pro Asp Pro Glu
565 570 575
Val Glu Thr Leu Ile Asn Asp Thr Leu Ala Lys Leu Gly Leu Lys Arg
580 585 590
Arg Asp Ile Thr Asp Glu Glu Ile Leu Glu Arg Met Val Tyr Pro Met
595 600 605
Ile Asn Glu Gly Ala Arg Ile Leu Glu Glu Lys Ile Ala Ala Arg Pro
610 615 620
Ser Asp Ile Asp Val Val Trp Leu Tyr Gly Tyr Gly Trp Pro Ile Tyr
625 630 635 640
Arg Gly Gly Pro Met His Tyr Ala Asp Ser Val Gly Leu Lys His Ile
645 650 655
Ala Glu Arg Leu Ser Ala Tyr Ala Lys Ala Thr Asn Asp Pro Ser Leu
660 665 670
Glu Pro Ala Pro Leu Leu Ala Arg Leu Ala Ala Glu Gly Lys Thr Phe
675 680 685
Ala Ser Leu Thr Gln Pro Ser Lys Ala Ala Ala
690 695
<210> 27
<211> 278
<212> PRT
<213> Penicillium chrysogenum
<400> 27
Met Ser Leu Leu Thr Thr Tyr Thr Thr Gln Asn Thr Thr Lys Ile Ala
1 5 10 15
His Leu Thr Ile Ser His Ala Gln Lys Leu Asn Ser Leu Thr Thr Pro
20 25 30
Leu Leu Thr Ser Leu Thr Gln Thr Leu Ser Lys Leu Ser Asn Thr Asn
35 40 45
Leu His Ala Ile Thr Leu Thr Gly Ala Gly Gln Lys Ser Phe Ile Gly
50 55 60
Gly Ala Asp Leu Asn Glu Leu Ser Thr Leu Arg Asn Ala Pro Thr Ala
65 70 75 80
Arg Lys Phe Ile Thr Ser Val His Glu Thr Cys Thr Ala Ile Arg Thr
85 90 95
Cys Pro Val Pro Val Ile Ala Arg Ile Asn Gly Phe Ala Leu Gly Ala
100 105 110
Gly Leu Glu Ile Ala Ala Ala Cys Asp Leu Arg Val Ala Ala Lys Gly
115 120 125
Ala Val Phe Gly Met Pro Glu Val Arg Leu Gly Ile Pro Ser Val Val
130 135 140
Glu Ala Ala Leu Leu Pro Gly Leu Ile Gly Trp Gly Arg Thr Arg Gln
145 150 155 160
Leu Leu Leu Leu Gly Gly Met Ile Ser Ala Ser Glu Ala Leu Arg Trp
165 170 175
Gly Leu Val Glu Arg Val Val Glu Asp Glu Glu Leu Asp Leu Ala Val
180 185 190
Ala Glu Trp Thr Ser Glu Ile Gly Arg Asn Gly Pro Leu Ala Val Arg
195 200 205
Arg Gln Lys Ala Leu Ile Ser Arg Trp Glu Glu Leu Ser Leu Ala Gly
210 215 220
Gly Ile Glu Ala Gly Ile Glu Ala Phe Gly Glu Cys Phe Asp Gly Asp
225 230 235 240
Cys Gly Thr Glu Pro Gly Arg Met Ile Gly Glu Phe Phe Arg Glu Lys
245 250 255
Glu Arg Ser Lys Ala Arg Ile Glu Ala Arg Asn Lys Val Val Ala Gly
260 265 270
Ser Lys Gly Glu Glu Gly
275
<210> 28
<211> 244
<212> PRT
<213> Penicillium chrysogenum
<400> 28
Met Thr Glu Ile Pro Thr Phe His Asn Leu Ser Leu Glu Arg His Gly
1 5 10 15
Asn Val Phe Val Leu Thr Met Gln Lys Pro Pro Glu Asn Arg Leu Asn
20 25 30
Ser Ser Tyr Cys Gln Glu Met Ile Arg Ala Tyr Arg Ser Val Glu Arg
35 40 45
Ile Leu Gly Ser Asp Ser Glu Gly Ala Val Ile Thr Arg Gly Asn Asp
50 55 60
Ala Lys Phe Trp Cys Thr Gly Leu Glu Leu Asp Glu Ser Asp Ser Asn
65 70 75 80
Pro Phe Ala Asn Thr Asp Gly Phe Tyr Pro Leu Ile His Thr Ile Leu
85 90 95
Asp Phe Pro Phe Pro Thr Val Ala Leu Leu Thr Gly His Thr Phe Gly
100 105 110
Gly Ala Cys Pro Leu Ala Leu Ala His Asp Tyr Arg Ile Met Asn Ser
115 120 125
Arg Arg Gly Phe Ile Ser Met Pro Pro Val Asn Leu Gly Leu His Phe
130 135 140
Asp Gly Ile Gly Ser Leu Pro Arg Leu Lys Leu Arg Pro Gln Val Ala
145 150 155 160
Arg Lys Met Leu Leu Glu Ala His Arg Trp Thr Gly Pro Glu Ala Leu
165 170 175
Glu Asp Gly Ile Val Asp Ala Val Ala Glu Pro Glu Asp Met Leu Asn
180 185 190
Val Ala Leu Glu Leu Gly Ala Lys Trp Ala Pro Lys Ala Lys Met Gly
195 200 205
Val Tyr Ala Leu Leu Arg Gln Glu Leu Trp Gly Asp Ala Ile Lys Lys
210 215 220
Phe Gln Arg Ile Ser Tyr Val His Ser Arg Val Thr Ser Ala Pro Ala
225 230 235 240
Lys Val Lys Ile
<210> 29
<211> 300
<212> PRT
<213> Penicillium chrysogenum
<400> 29
Met Ala Pro Ser Asp Arg Leu Thr Gln Val Asn Glu His Leu Asn Tyr
1 5 10 15
Pro Ala Gly Leu Leu Ala Gly Gln Val Ala Ile Ile Thr Gly Ala Gly
20 25 30
Gln Gly Ile Gly Ala Glu Ala Ala Arg Leu Phe Ala Asn Glu Gly Ala
35 40 45
Lys Val Val Val Ala Asp Ile Asp Ser Lys Lys Ala Asn Ala Val Ala
50 55 60
Asp Ala Ile Asn Ser Ala Lys Ala Gly Arg Ala Leu Ala Val Val Gly
65 70 75 80
Asp Val Leu Asp Ser Asn Tyr Ile Thr Glu Leu Val Lys Lys Thr Ala
85 90 95
Glu Phe Gly Asn Gly Lys Ile His Ile Ile Val Asn Asn Ala Gly Phe
100 105 110
Thr Trp Asp Gly Val Ile His Lys Met Thr Asp Lys Gln Trp Glu Thr
115 120 125
Met Leu Ala Val His Asn Thr Ala Pro Phe Gln Leu Val Arg Ala Ala
130 135 140
Ala Pro Tyr Phe Arg Val Lys Asp Gln Glu Pro Arg Val Val Ile Asn
145 150 155 160
Ile Ser Ser Thr Ser Gly Val His Gly Asn Ala Gly Gln Ala Asn Tyr
165 170 175
Ala Val Ala Lys Ala Gly Val Val Gly Leu Thr Arg Thr Ile Ala Lys
180 185 190
Glu Trp Gly Pro Ser Phe Gly Val Arg Ser Asn Thr Ile Ala Phe Gly
195 200 205
Phe Val Thr Thr Arg Leu Thr Ala Ala Lys Glu Glu Gly Ala Phe Ile
210 215 220
Thr Thr Pro Asp Gly Thr Lys Val Ala Leu Gly Ile Pro Gly Lys Gln
225 230 235 240
Leu Ala Thr Lys Lys Gly Ser Ala Asp Gln Glu Lys Lys Ala Ala Pro
245 250 255
Thr Tyr Pro Asp Ile Pro Leu Gly Arg Pro Ala Thr Pro Glu Glu Ala
260 265 270
Ala Arg Ala Val Leu Gly Val Ala Ser Pro Trp Phe Ser Tyr Val Asn
275 280 285
Gly Glu Thr Ile Arg Val Thr Gly Gly Arg Asn Met
290 295 300
<210> 30
<211> 327
<212> PRT
<213> Penicillium chrysogenum
<400> 30
Met Ser Ala Pro Gly Ala Gly His Glu Phe Pro Ala Gln Glu Val Ser
1 5 10 15
Trp Gln Lys Arg Asp Val Leu Leu Phe Ala Asn Ser Ile Gly Val Lys
20 25 30
Ala Asp Glu Leu His Phe Leu Tyr Glu Leu His Pro Asn Phe Ala Val
35 40 45
Phe Pro Thr Tyr Ser Leu Ile Leu Arg Met Ser Lys Asn Thr Ser Asp
50 55 60
Phe Arg Thr Ile Ala Leu Thr Pro Lys Pro Leu Ala Phe Lys Leu Thr
65 70 75 80
Asp Gln Glu Val Thr Asp Phe Tyr Ala Arg Gln Lys Ala Val His Ile
85 90 95
Pro Gly Val Pro Asp Leu Asp His Arg His Gly Val Asp Gly Gln Arg
100 105 110
Lys Ile Thr Ile Leu Lys Pro Leu Pro Thr Thr Ser Ala Gly Arg Lys
115 120 125
Phe Glu Leu Arg Asn Lys Val Ile Gly Val Tyr Asp Lys Gly Lys Pro
130 135 140
Gly Thr Val Ile Glu Thr Glu Gln Ser Ile Val Asp Lys Glu Ser Gly
145 150 155 160
Glu Val Tyr Ser Lys Val Val Ser Ser Gly Phe Leu Val Gly Gln Gly
165 170 175
Gly Trp Gly Gly Pro Lys Gly Pro Ser Thr Val Asn Tyr Ala Pro Pro
180 185 190
Glu Gly Arg Ala Pro Asp Ala Thr His Val Val Gln Ser Asn Ser Glu
195 200 205
Thr Ala His Leu Tyr Arg Leu Asn Gly Asp Tyr Asn Pro Leu His Ala
210 215 220
Thr Pro Glu Pro Gly Gln Lys Met Gly Phe Gly Gly Ile Ile Ile His
225 230 235 240
Gly Leu Phe Ser Trp Asn Ser Ala Ala His Gly Ile Leu Arg Glu Phe
245 250 255
Gly Gly Ser Asn Pro Ala Asn Met Lys Glu Phe Gln Ala Arg Phe Ala
260 265 270
Ser Pro Val Arg Pro Gly Asp Lys Leu Thr Thr Glu Ile Trp Arg Met
275 280 285
Gly Asn Ile Gln Asp Gly Tyr Glu Glu Ile Arg Phe Val Thr Lys Asn
290 295 300
Asp Lys Gly Arg Val Val Leu Ser Asn Gly Arg Cys Leu Leu Lys Val
305 310 315 320
Thr Gly Val Lys Ser Lys Leu
325
<210> 31
<211> 901
<212> PRT
<213> Penicillium chrysogenum
<400> 31
Met Ser Glu Leu Arg Phe Asp Asn Gln Thr Val Val Val Thr Gly Ala
1 5 10 15
Gly Gly Gly Leu Gly Lys Ala Tyr Ala Leu Phe Phe Ala Ser Arg Gly
20 25 30
Ala Asn Val Val Val Asn Asp Leu Gly Ala Ser His Lys Gly Glu Gly
35 40 45
Lys Ser Gly Lys Ala Ala Asp Val Val Val Glu Glu Ile Arg Ala Ala
50 55 60
Gly Gly Lys Ala Val Ala Asn Tyr Asp Ser Val Glu Asn Gly Asp Ala
65 70 75 80
Ile Ile Glu Thr Ala Ile Lys Ala Phe Gly Arg Ile Asp Ile Leu Leu
85 90 95
Asn Asn Ala Gly Ile Leu Arg Asp Ile Ser Phe Lys Asn Met Lys Asp
100 105 110
Ala Asp Trp Asp Leu Ile Asn Arg Val His Thr Tyr Gly Ala Tyr Lys
115 120 125
Cys Ala Arg Ala Ala Trp Pro His Phe Arg Lys Gln Lys Phe Gly Arg
130 135 140
Val Ile Asn Thr Ala Ser Ala Ala Gly Leu Phe Gly Ser Phe Gly Gln
145 150 155 160
Ala Asn Tyr Ser Ala Ala Lys Leu Gly Gln Val Gly Phe Thr Glu Thr
165 170 175
Leu Ala Lys Glu Gly Ala Lys Tyr Asn Ile Ile Ala Asn Val Ile Ala
180 185 190
Pro Ile Ala Ala Ser Arg Met Thr Ala Thr Val Met Pro Pro Asp Val
195 200 205
Leu Glu Asn Leu Lys Pro Asp Trp Val Val Pro Leu Val Ala Ala Leu
210 215 220
Val His Ser Ser Asn Thr Thr Glu Thr Gly Gly Ile Tyr Glu Val Gly
225 230 235 240
Gly Gly His Val Ala Lys Leu Arg Trp Glu Arg Ala Lys Gly Ala Leu
245 250 255
Leu Lys Thr Asp Asp Ser Leu Thr Pro Gly Ala Ile Ala Arg Lys Trp
260 265 270
Asn Asp Val Asn Asp Phe Ser Gln Pro Glu Tyr Pro Thr Gly Pro Ala
275 280 285
Asp Phe Met Ala Phe Leu Glu Asp Gly Leu Lys Thr Pro Ser Ala Gln
290 295 300
Pro Gly Gln Glu Pro Asp Phe Lys Gly Arg Val Ala Leu Val Thr Gly
305 310 315 320
Gly Gly Ala Gly Leu Gly Arg Ala Tyr Cys Leu Gln Phe Ala Lys Leu
325 330 335
Gly Ala Ser Val Val Val Asn Asp Leu Val Asp Pro Glu Pro Val Val
340 345 350
Gln Glu Ile Lys Lys Leu Gly Gly Lys Ala Val Gly Asn Lys Ala Ser
355 360 365
Cys Glu Asp Gly Asp Ala Val Val Lys Ser Ala Ile Asp Ala Phe Gly
370 375 380
Arg Ile Asp Ile Leu Val Asn Asn Ala Gly Ile Leu Arg Asp Lys Ala
385 390 395 400
Phe Thr Asn Met Asp Asp Asn Leu Trp Asn Ser Val Val Asn Val His
405 410 415
Leu Arg Gly Thr Tyr Lys Val Thr Lys Ala Ala Trp Pro Tyr Phe Leu
420 425 430
Lys Gln Lys Tyr Gly Arg Val Val Asn Thr Ala Ser Thr Ser Gly Ile
435 440 445
Tyr Gly Asn Phe Gly Gln Ala Asn Tyr Ala Ala Ala Lys Leu Gly Ile
450 455 460
Leu Gly Leu Ser Arg Thr Leu Ala Leu Glu Gly Ala Lys Tyr Asn Ile
465 470 475 480
Lys Val Asn Thr Ile Ala Pro Asn Ala Gly Thr Asn Met Thr Arg Thr
485 490 495
Ile Met Pro Glu Glu Met Val Gln Ala Phe Lys Pro Asp Tyr Val Ala
500 505 510
Pro Leu Val Val Leu Leu Cys Ser Asp Met Ala Pro Glu Pro Ser Thr
515 520 525
Lys Gly Leu Phe Glu Cys Gly Ser Gly Trp Phe Gly Arg Thr Arg Trp
530 535 540
Gln Arg Thr Gly Gly His Gly Phe Pro Val Asp Val Lys Leu Thr Pro
545 550 555 560
Glu Glu Val Val Arg Asn Trp Lys Gln Ile Ile Asn Phe Asp Asp Gly
565 570 575
Arg Ala Asp His Pro Glu Asp Gly Gln Ala Gly Ala Glu Lys Ile Met
580 585 590
Ala Asn Met Ser Asn Arg Val His Gly Asp Thr Ser Thr Glu Asn Glu
595 600 605
Thr Leu Lys Asn Ile Lys Lys Ala Lys Ala Leu Ser Ser Glu Gly Thr
610 615 620
Pro Phe Asn Tyr Glu Asp Arg Asp Val Ile Leu Tyr Asn Leu Ser Leu
625 630 635 640
Gly Ala Lys Arg Thr Asp Leu Pro Leu Val Tyr Glu Asn Asn Asp Gln
645 650 655
Phe Gln Ala Leu Pro Ser Tyr Gly Val Val Pro Trp Phe Asn Thr Ala
660 665 670
Thr Pro Trp Asn Met Asp Asp Leu Val Lys Asp Phe Ser Pro Met Met
675 680 685
Leu Leu His Gly Glu Gln Tyr Met Glu Val Arg Lys Phe Pro Ile Pro
690 695 700
Thr Thr Ala Asn Thr Leu Thr Tyr Pro Lys Leu Ile Asp Val Ile Asp
705 710 715 720
Lys Gly Asn Ala Ala Ile Val Val Ala Gly Tyr Thr Thr Lys Asp Ala
725 730 735
Lys Thr Gly Glu Asp Leu Phe Tyr Asn Glu Ser Ser Val Phe Ile Arg
740 745 750
Gly Ser Gly Gly Phe Gly Gly Ser Pro Lys Pro Thr Ala Val Arg Pro
755 760 765
Lys Ala Ala Thr Ala Ala Tyr Lys Ala Pro Gln Arg Gln Pro Asp Ala
770 775 780
Val Val Glu Glu Lys Thr Ser Glu Asp Gln Ala Ala Leu Tyr Arg Leu
785 790 795 800
Asn Gly Asp Arg Asn Pro Leu His Ile Asp Pro Glu Phe Ser Lys Val
805 810 815
Gly Gly Phe Lys Thr Pro Ile Leu His Gly Leu Cys Ser Leu Gly Val
820 825 830
Ser Ala Lys Ala Val Phe Ser Lys Tyr Gly Pro Tyr Lys Asn Leu Lys
835 840 845
Val Arg Phe Ala Gly Val Val Leu Pro Gly Gln Thr Leu Lys Thr Glu
850 855 860
Met Trp Lys Glu Gly Asn Thr Val Leu Phe Gln Ala Thr Val Val Glu
865 870 875 880
Thr Gly Lys Pro Ala Ile Thr Gly Ala Gly Ala Glu Leu Leu Glu Gly
885 890 895
Ala Lys Ala Lys Leu
900
<210> 32
<211> 293
<212> PRT
<213> Penicillium chrysogenum
<400> 32
Met Phe Ala Arg Gln Cys Thr Arg Leu Ala Thr Ser Arg Thr Ser Ala
1 5 10 15
Pro Leu Thr Ser Tyr Leu Ala Arg Val Arg Gly Tyr Ser Ser Ala Ala
20 25 30
Gly Ser Tyr Glu His Ile Leu Thr Ser Thr Pro Lys Pro Gly Val Gly
35 40 45
Leu Ile Thr Leu Asn Arg Pro Lys Ala Leu Asn Ala Leu Cys Ser Pro
50 55 60
Leu Phe Lys Glu Leu Asn Glu Ala Leu Ser Asn Tyr Asp Asn Asp Lys
65 70 75 80
Ser Ile Gly Ala Ile Ile Ile Thr Gly Ser Glu Lys Ala Phe Ala Ala
85 90 95
Gly Ala Asp Ile Lys Glu Met Ala Pro Leu Ser Phe Ser Ala Ala Tyr
100 105 110
Ser Asp Asn Phe Ile Ala Pro Trp Ser His Leu Ala Thr Ser Ile Arg
115 120 125
Thr Pro Val Ile Ala Ala Val Ser Gly Tyr Ala Leu Gly Gly Gly Cys
130 135 140
Glu Leu Ala Leu Met Cys Asp Ile Leu Tyr Cys Ser Glu Asn Ala Thr
145 150 155 160
Phe Gly Gln Pro Glu Ile Lys Leu Gly Thr Ile Pro Gly Ala Gly Gly
165 170 175
Ser Gln Arg Leu Thr Arg Ala Ile Gly Lys Ser Lys Ala Met Glu Leu
180 185 190
Ile Leu Thr Gly Lys Asn Phe Ser Gly Lys Glu Ala Gly Glu Trp Gly
195 200 205
Val Ala Ala Lys Val Val Pro Gly Gly Lys Glu Glu Leu Leu Glu Gln
210 215 220
Ala Tyr Lys Thr Ala Glu Thr Ile Ala Ser Tyr Ser Arg Val Ala Val
225 230 235 240
Val Ala Gly Lys Glu Val Val Asn Lys Ser Gln Glu Leu Ser Leu Lys
245 250 255
Glu Gly Val Glu Tyr Glu Arg Arg Leu Phe His Ala Leu Phe Gly Ser
260 265 270
Lys Asp Gln Lys Ile Gly Met Thr Ala Phe Ala Glu Lys Lys Lys Pro
275 280 285
Glu Trp Ser His Glu
290
<210> 33
<211> 261
<212> PRT
<213> Acinetobacter sp.
<400> 33
Met Ile Pro Asp Gln Asp Asn Phe Val Glu Ile Asp Phe Ser Ile Glu
1 5 10 15
Gln Ile Ala Ile Val Lys Ile Asn Arg Pro Ala Ser Lys Asn Ala Leu
20 25 30
Asn Thr Glu Val Arg Lys Gln Leu Ala Gln Ala Phe Thr Glu Leu Ser
35 40 45
Phe Asn Asp Gln Ile Asn Ala Ile Val Leu Thr Gly Gly Glu Asp Val
50 55 60
Phe Ala Ala Gly Ala Asp Leu Lys Glu Met Ala Thr Ala Ser Ser Thr
65 70 75 80
Asp Met Leu Leu Arg His Thr Glu Arg Tyr Trp Asn Ala Ile Ala Gln
85 90 95
Cys Pro Lys Pro Val Ile Ala Ala Val Asn Gly Tyr Ala Leu Gly Gly
100 105 110
Gly Cys Glu Leu Ala Met His Thr Asp Ile Ile Ile Ala Gly Lys Ser
115 120 125
Ala Thr Phe Gly Gln Pro Glu Ile Lys Val Gly Leu Met Pro Gly Ala
130 135 140
Gly Gly Thr Gln Arg Leu Phe Arg Ala Val Gly Lys Phe His Ala Met
145 150 155 160
Arg Met Ile Met Thr Gly Val Met Val Pro Ala Glu Glu Ala Tyr Leu
165 170 175
Ile Gly Leu Val Ser Gln Val Thr Glu Asp Ser Gln Thr Ile Pro Thr
180 185 190
Ala Ile Lys Met Ala Gln Ser Leu Ala Lys Met Pro Pro Ile Ala Leu
195 200 205
Gln Gln Ile Lys Glu Val Ala Leu Met Ser Glu Asp Val Pro Leu Asn
210 215 220
Ala Gly Leu Thr Leu Glu Arg Lys Ser Phe Gln Leu Leu Phe Ser Thr
225 230 235 240
Glu Asp Lys Asn Glu Gly Ile Asn Ala Phe Ile Glu Lys Arg Lys Pro
245 250 255
Ser Tyr His Gly Lys
260
<210> 34
<211> 136
<212> PRT
<213> Aeromonas salmonicida
<400> 34
Met Asn Thr Leu Pro Leu Glu Ile Gly Gln Lys Ala Ser Leu Thr Lys
1 5 10 15
Arg Phe Gly Ala Ala Glu Val Glu Ala Phe Ala Gly Leu Ser Glu Asp
20 25 30
Phe Asn Pro Leu His Leu Asp Ser Ala Phe Ala Ala Thr Thr Pro Phe
35 40 45
Glu Arg Pro Ile Val His Gly Met Leu Leu Ala Ser Leu Phe Ser Gly
50 55 60
Leu Leu Gly Gln Gln Leu Pro Gly Lys Gly Thr Val Tyr Leu Gly Gln
65 70 75 80
Ser Leu Ser Phe Lys Gln Pro Val Phe Val Gly Asp Glu Val Thr Ala
85 90 95
Glu Val Glu Ile Ile Ala Met Arg Ser Asp Lys Pro Ile Ile Thr Leu
100 105 110
Ala Thr Arg Ile Leu Ala Ala Gly Gly Ala Leu Ala Leu Ala Val Thr
115 120 125
Gly Glu Ala Val Val Lys Val Gly
130 135
<210> 35
<211> 259
<212> PRT
<213> Clostridium kluyveri
<400> 35
Met Glu Phe Lys Asn Ile Ile Leu Glu Lys Asp Gly Asn Val Ala Ser
1 5 10 15
Ile Thr Leu Asn Arg Pro Lys Ala Leu Asn Ala Leu Asn Ala Ala Thr
20 25 30
Leu Lys Glu Ile Asp Ala Ala Ile Asn Asp Ile Ala Glu Asp Asp Asn
35 40 45
Val Tyr Ala Val Ile Ile Thr Gly Ser Gly Lys Ala Phe Val Ala Gly
50 55 60
Ala Asp Ile Ala Glu Met Lys Asp Leu Thr Ala Val Glu Gly Arg Lys
65 70 75 80
Phe Ser Val Leu Gly Asn Lys Ile Phe Arg Lys Leu Glu Asn Leu Glu
85 90 95
Lys Pro Val Ile Ala Ala Ile Asn Gly Phe Ala Leu Gly Gly Gly Cys
100 105 110
Glu Leu Ser Leu Ser Cys Asp Ile Arg Ile Ala Ser Ser Lys Ala Lys
115 120 125
Phe Gly Gln Pro Glu Val Gly Leu Gly Ile Thr Pro Gly Phe Gly Gly
130 135 140
Thr Gln Arg Leu Ala Arg Ala Ile Gly Val Gly Met Ala Lys Glu Leu
145 150 155 160
Ile Tyr Thr Gly Lys Val Ile Asn Ala Glu Glu Ala Leu Arg Ile Gly
165 170 175
Leu Val Asn Lys Val Val Glu Pro Asp Lys Leu Leu Glu Glu Ala Lys
180 185 190
Ala Leu Val Asp Ala Ile Ile Val Asn Ala Pro Ile Ala Val Arg Met
195 200 205
Cys Lys Ala Ala Ile Asn Gln Gly Leu Gln Cys Asp Ile Asp Thr Gly
210 215 220
Val Ala Tyr Glu Ala Glu Val Phe Gly Glu Cys Phe Ala Thr Glu Asp
225 230 235 240
Arg Val Glu Gly Met Thr Ala Phe Val Glu Lys Arg Asp Lys Ala Phe
245 250 255
Lys Asn Lys
<210> 36
<211> 257
<212> PRT
<213> Clostridium kluyveri
<400> 36
Met Thr Tyr Lys Thr Leu Leu Leu Glu Lys Gln Asn Gly Ile Thr Ile
1 5 10 15
Ile Lys Met Asn Thr Pro His Asn Leu Asn Ala Ile Ser Gln Gln Ser
20 25 30
Val Glu Asp Leu Phe Ala Val Leu Gln Val Ile Lys Asn Asp Asp Asn
35 40 45
Cys Arg Val Val Ile Leu Thr Gly Glu Gly Lys Gly Phe Ile Gly Gly
50 55 60
Ala Asp Ile Lys His Met Ala Cys Leu Asp Ala Ile Glu Gly Gly Gln
65 70 75 80
Phe Cys Phe Ala Val Ser Lys Cys Thr Leu Glu Met Glu Lys Met Gly
85 90 95
Lys Val Phe Ile Ala Ala Val Asn Gly Phe Ala Leu Gly Ala Gly Leu
100 105 110
Glu Val Ala Leu Gly Cys Asp Ile Arg Ile Phe Ser Lys His Ala Lys
115 120 125
Ile Gly Phe Pro Glu Thr Gly Leu Gly Val Ile Pro Gly Ala Gly Gly
130 135 140
Ala Gln Arg Leu Gln Arg Leu Val Gly Ile Gly Lys Ala Ser Glu Ile
145 150 155 160
Ile Phe Thr Gly Asp Ile Ile Gly Ala Asp Asp Ala Leu Arg Phe Gly
165 170 175
Ile Ala Asn Gln Val Thr Glu Pro Glu Ser Leu Met Asp Thr Ala Met
180 185 190
Ser Met Ala Glu Lys Ile Leu Thr Lys Ser Pro Val Gly Thr Arg Leu
195 200 205
Ala Lys Glu Ala Leu Gln Lys Gly Arg Asp Thr Asp Leu Glu Lys Ala
210 215 220
Leu Glu Tyr Asp Lys Asn Leu Phe Gly Leu Cys Phe Ser Thr Glu Asp
225 230 235 240
Lys Lys Glu Gly Met Ala Ala Phe Ile Glu Lys Arg Lys Pro Val Phe
245 250 255
Lys
<210> 37
<211> 255
<212> PRT
<213> Escherichia coli
<400> 37
Met Ser Glu Leu Ile Val Ser Arg Gln Gln Arg Val Leu Leu Leu Thr
1 5 10 15
Leu Asn Arg Pro Ala Ala Arg Asn Ala Leu Asn Asn Ala Leu Leu Met
20 25 30
Gln Leu Val Asn Glu Leu Glu Ala Ala Ala Thr Asp Thr Ser Ile Ser
35 40 45
Val Cys Val Ile Thr Gly Asn Ala Arg Phe Phe Ala Ala Gly Ala Asp
50 55 60
Leu Asn Glu Met Ala Glu Lys Asp Leu Ala Ala Thr Leu Asn Asp Thr
65 70 75 80
Arg Pro Gln Leu Trp Ala Arg Leu Gln Ala Phe Asn Lys Pro Leu Ile
85 90 95
Ala Ala Val Asn Gly Tyr Ala Leu Gly Ala Gly Cys Glu Leu Ala Leu
100 105 110
Leu Cys Asp Val Val Val Ala Gly Glu Asn Ala Arg Phe Gly Leu Pro
115 120 125
Glu Ile Thr Leu Gly Ile Met Pro Gly Ala Gly Gly Thr Gln Arg Leu
130 135 140
Ile Arg Ser Val Gly Lys Ser Leu Ala Ser Lys Met Val Leu Ser Gly
145 150 155 160
Glu Ser Ile Thr Ala Gln Gln Ala Gln Gln Ala Gly Leu Val Ser Asp
165 170 175
Val Phe Pro Ser Asp Leu Thr Leu Glu Tyr Ala Leu Gln Leu Ala Ser
180 185 190
Lys Met Ala Arg His Ser Pro Leu Ala Leu Gln Ala Ala Lys Gln Ala
195 200 205
Leu Arg Gln Ser Gln Glu Val Ala Leu Gln Ala Gly Leu Ala Gln Glu
210 215 220
Arg Gln Leu Phe Thr Leu Leu Ala Ala Thr Glu Asp Arg His Glu Gly
225 230 235 240
Ile Ser Ala Phe Leu Gln Lys Arg Thr Pro Asp Phe Lys Gly Arg
245 250 255
<210> 38
<211> 257
<212> PRT
<213> Pseudomonas putida
<400> 38
Met Pro Arg Tyr Leu Asp Val Gln Ala Pro Glu Asn Gly Val Gln Leu
1 5 10 15
Ile Thr Leu Gln Arg Pro Glu Ala Leu Asn Ala Leu Cys Thr Glu Leu
20 25 30
Leu Ala Glu Leu Ala Thr Ala Leu Asp Ala Ala Ala Arg Asp Asp Gln
35 40 45
Ile Gly Val Val Val Leu Thr Gly Ser Arg Lys Ala Phe Ala Ala Gly
50 55 60
Ala Asp Ile Arg Glu Met Ala Glu Arg Asp Leu Val Gly Ile Leu Asn
65 70 75 80
Asp Pro Arg Val Ala His Trp Gln Arg Ile Ala Ala Phe Ala Lys Pro
85 90 95
Leu Ile Ala Ala Val Asn Gly Tyr Ala Leu Gly Gly Gly Cys Glu Leu
100 105 110
Val Met Cys Ala Asp Ile Val Ile Ala Gly Ser Asp Ala Arg Phe Gly
115 120 125
Gln Pro Glu Ile Asn Leu Gly Ile Ile Pro Gly Ala Gly Gly Thr Gln
130 135 140
Arg Leu Leu Arg Ala Val Gly Lys Pro Leu Ala Met Gln Met Val Leu
145 150 155 160
Thr Gly Glu Ala Ile Thr Ala Arg His Ala Gln Gln Ala Gly Leu Val
165 170 175
Ser Glu Ile Thr Gln Pro Glu Phe Thr Val Glu Arg Ala Met Gln Ile
180 185 190
Ala Arg Asn Ile Ala Ala Lys Ala Pro Leu Ala Val Arg Leu Ala Lys
195 200 205
Glu Ala Leu Leu Lys Ala Gly Asp Thr Asp Leu Ala Ser Gly Leu Arg
210 215 220
Phe Glu Arg His Ala Phe Thr Leu Leu Ala Gly Thr Ala Asp Arg Asp
225 230 235 240
Glu Gly Ile Gln Ala Phe Gln Glu Lys Arg Pro Ala Arg Phe Gln Gly
245 250 255
Arg
<210> 39
<211> 263
<212> PRT
<213> Pseudomonas putida
<400> 39
Met Thr Phe Gln His Ile Leu Phe Ser Ile Glu Asp Gly Val Ala Phe
1 5 10 15
Leu Ser Leu Asn Arg Pro Glu Gln Leu Asn Ser Phe Asn Ala Ala Met
20 25 30
His Leu Glu Val Arg Glu Ala Leu Lys Gln Val Arg Gln Ser Ser Asp
35 40 45
Ala Arg Val Leu Leu Leu Thr Ala Glu Gly Arg Gly Phe Cys Ala Gly
50 55 60
Gln Asp Leu Ser Asp Arg Asn Val Ala Pro Asp Ala Glu Val Pro Asp
65 70 75 80
Leu Gly Glu Ser Ile Asp Lys Phe Tyr Asn Pro Leu Val Arg Thr Leu
85 90 95
Arg Asp Leu Pro Leu Pro Val Ile Cys Ala Val Asn Gly Val Ala Ala
100 105 110
Gly Ala Gly Ala Asn Ile Pro Leu Ala Cys Asp Leu Val Leu Ala Gly
115 120 125
Arg Ser Ala Ser Phe Ile Gln Ala Phe Cys Lys Ile Gly Leu Val Pro
130 135 140
Asp Ser Gly Gly Thr Trp Leu Leu Pro Arg Leu Val Gly Met Ala Arg
145 150 155 160
Ala Lys Ala Leu Ala Met Leu Gly Glu Arg Leu Gly Ala Glu Gln Ala
165 170 175
Gln Gln Trp Gly Leu Ile His Arg Val Val Asp Asp Ala Ala Leu Arg
180 185 190
Asp Glu Ala Leu Thr Leu Ala Arg Gln Leu Ala Ser Gln Pro Thr Tyr
195 200 205
Gly Leu Ala Leu Ile Lys Arg Ser Leu Asn Ala Ser Phe Asp Asn Gly
210 215 220
Phe Asp Glu Gln Leu Glu Leu Glu Arg Asp Leu Gln Arg Leu Ala Gly
225 230 235 240
Arg Ser Glu Asp Tyr Arg Glu Gly Val Ser Ala Phe Met Asn Lys Arg
245 250 255
Thr Pro Ala Phe Lys Gly Arg
260
<210> 40
<211> 699
<212> PRT
<213> Rhodopseudomonas palustris
<400> 40
Met Ser Glu Val Val Thr Arg Ala Thr Gln Asp Gln Val Ala Ile Val
1 5 10 15
Thr Val Asp Ser Pro Pro Val Asn Ala Leu Ser Ala Ala Val Arg Arg
20 25 30
Gly Ile Leu Glu Asn Val Asn Ala Ala Val Ala Asp Pro Ala Val Gln
35 40 45
Ala Ile Val Leu Val Cys Ala Gly Arg Thr Phe Ile Ala Gly Ala Asp
50 55 60
Ile Thr Glu Phe Gly Lys Pro Pro Gln Pro Pro Ala Leu Asn Asp Val
65 70 75 80
Ile Ala Ala Leu Glu Asn Ser Pro Lys Pro Thr Ile Ala Ala Ile His
85 90 95
Gly Thr Ala Leu Gly Gly Gly Leu Glu Val Ala Leu Gly Cys His Phe
100 105 110
Arg Val Ala Val Lys Glu Ala Lys Leu Gly Leu Pro Glu Val Lys Leu
115 120 125
Gly Leu Leu Pro Gly Ala Gly Gly Thr Gln Arg Leu Pro Arg Ala Val
130 135 140
Gly Pro Glu Leu Ala Val Gln Met Ile Val Gly Gly Ser Pro Ile Gly
145 150 155 160
Ala Ala Glu Ala Leu Lys His Gly Leu Val Glu Glu Val Val Glu Asn
165 170 175
Leu Val Ala Gly Ala Val Ala Phe Ala Lys Lys Val Leu Ala Glu Lys
180 185 190
Arg Pro Leu Arg Arg Leu Arg Asp Asp Asp Ser Lys Leu Ala Ala Ala
195 200 205
Lys Ala Asp Arg Ser Ile Phe Thr Asn Ala Val Ala Ala Met Thr Lys
210 215 220
Lys Ala Arg Gly Leu Glu Ala Pro Phe Ala Cys Ala Asp Ala Ile Gly
225 230 235 240
Ala Ala Ile Asp Leu Pro Phe Glu Glu Gly Leu Lys Lys Glu Arg Glu
245 250 255
Gly Phe Met Lys Leu Val Val Ser Asp Gln Ser Lys Ala Gln Arg Tyr
260 265 270
Ala Phe Phe Ala Glu Arg Glu Ala Ala Lys Val Asp Gly Val Pro Asp
275 280 285
Gly Thr Lys Pro Arg Pro Val Ser Arg Val Ala Ile Ile Gly Ala Gly
290 295 300
Thr Met Gly Gly Gly Ile Ala Met Ser Phe Ala Asn Ala Gly Ile Pro
305 310 315 320
Val Thr Leu Ile Glu Thr Gly Glu Glu Gln Leu Lys Arg Gly Leu Gly
325 330 335
Ile Met Gln Lys Asn Trp Glu Ala Thr Ala Ala Arg Gly Gly Leu Pro
340 345 350
Pro Asp Ala Pro Ala Lys Arg Met Ala Leu Ile Thr Gly Leu Val Gly
355 360 365
Leu Glu Asn Val Lys Asp Ala Asp Leu Ile Ile Glu Ala Val Phe Glu
370 375 380
Thr Met Ala Val Lys Lys Glu Val Phe Thr Ala Val Asp Ala His Ala
385 390 395 400
Lys Pro Gly Ala Val Leu Ala Ser Asn Thr Ser Tyr Leu Ser Ile Asp
405 410 415
Glu Ile Ala Ala Thr Thr Lys Arg Pro Gln Asp Val Leu Gly Met His
420 425 430
Phe Phe Ser Pro Ala Asn Val Met Lys Leu Cys Glu Ile Val Arg Gly
435 440 445
Ala Lys Thr Ala Pro Asp Ala Leu Leu Thr Ala Val Ser Ile Ala Lys
450 455 460
Lys Ile Ala Lys Val Pro Val Val Val Gly Val Cys Asp Gly Phe Val
465 470 475 480
Gly Asn Arg Met Leu Ala Ala Arg Ser Lys Gln Ser Glu Lys Leu Leu
485 490 495
Phe Glu Gly Ala Leu Pro Gln Gln Val Asp Ala Val Val Thr Lys Phe
500 505 510
Gly Met Pro Met Gly Pro Phe Ala Met Gly Asp Leu Ala Gly Leu Asp
515 520 525
Ile Gly Trp Arg Ser Arg Lys Asp Arg Gly Ile Lys Ser Glu Ile Ala
530 535 540
Asp Ala Leu Cys Glu Ala Gly Arg Phe Gly Gln Lys Thr Gly Lys Gly
545 550 555 560
Tyr Tyr Lys Tyr Glu Gln Gly Ser Arg Ala Pro Met Pro Asp Pro Glu
565 570 575
Val Glu Thr Leu Ile Asn Asp Thr Leu Ala Lys Leu Gly Leu Lys Arg
580 585 590
Arg Asp Ile Thr Asp Glu Glu Ile Leu Glu Arg Met Val Tyr Pro Met
595 600 605
Ile Asn Glu Gly Ala Arg Ile Leu Glu Glu Lys Ile Ala Ala Arg Pro
610 615 620
Ser Asp Ile Asp Val Val Trp Leu Tyr Gly Tyr Gly Trp Pro Ile Tyr
625 630 635 640
Arg Gly Gly Pro Met His Tyr Ala Asp Ser Val Gly Leu Lys His Ile
645 650 655
Ala Glu Arg Leu Ser Ala Tyr Ala Lys Ala Thr Asn Asp Pro Ser Leu
660 665 670
Glu Pro Ala Pro Leu Leu Ala Arg Leu Ala Ala Glu Gly Lys Thr Phe
675 680 685
Ala Ser Leu Thr Gln Pro Ser Lys Ala Ala Ala
690 695
<210> 41
<211> 242
<212> PRT
<213> Rhodococcus sp.
<400> 41
Met Asn Thr Leu Lys Ile Glu Asp Arg Asp Asp Arg Met Val Val Thr
1 5 10 15
Leu His Arg Pro Ala Gln Arg Asn Ala Ile Asn Ala Glu Met Ile Gly
20 25 30
Glu Leu His Gln Val Cys Ala Ser Leu Glu Ala Thr Pro Lys Pro Met
35 40 45
Leu Leu Thr Gly Glu Gly Asp His Phe Ala Gly Gly Ala Asp Ile Ala
50 55 60
Glu Leu Arg Glu Arg Gly Arg Asp Glu Ala Phe Ala Gly Ile Asn Arg
65 70 75 80
Asn Leu Phe Asp Arg Ile Ala Lys Leu Pro Met Pro Thr Val Ala Ala
85 90 95
Val Ser Gly Tyr Ala Leu Gly Gly Gly Ala Glu Leu Ser Tyr Ala Cys
100 105 110
Asp Ile Arg Ile Ala Thr Glu Thr Ala Val Phe Gly Asn Pro Glu Pro
115 120 125
Gly Leu Gly Ile Met Ala Ala Ala Gly Ala Ser Tyr Arg Leu Pro Glu
130 135 140
Leu Val Gly Thr Ser Val Ala Lys Gln Val Leu Leu Gly Gly Arg Asn
145 150 155 160
Leu Asp Ala Gln Asp Ala Leu Arg Ser Gly Leu Val Met Ser Val Val
165 170 175
Ala Pro Gly Glu His Ile Asp Ala Ala His Lys Val Ile Asp Arg Ile
180 185 190
Thr Arg Ser Ala Pro Leu Ala Leu Lys Leu Thr Lys Met Ile Val Asp
195 200 205
Ala Pro Gly Ser His Pro Phe Ala Asp Asp Ile Ala Gln Ala Val Leu
210 215 220
Phe Glu Ser Arg Asp Lys His Asp Arg Met Thr Ala Phe Leu Glu Lys
225 230 235 240
Lys Lys
<210> 42
<211> 689
<212> PRT
<213> Penicillium chrysogenum
<400> 42
Met Pro Asp Phe Thr Asp Asn Leu Arg Pro Ser Gln Pro Asp Gly Pro
1 5 10 15
Thr Thr Leu Ala Arg Glu Arg Gln Lys Ser Asn Ile Ala Thr Glu Glu
20 25 30
Leu Gly Gln His Leu Leu Gly Ser Asp Gly Phe Leu Val Arg Gln Ala
35 40 45
Arg Ile Leu Pro Ile Ile Gln Gln Glu Pro Leu Phe Ser Lys Asp Gln
50 55 60
Gln Gln Asn Leu Ser Arg Pro Glu Arg Phe Lys Leu Gly Val Ala Arg
65 70 75 80
Ala Lys Leu Leu Arg Gln Met Lys Asp Thr His Lys Trp Ser His Leu
85 90 95
Glu Tyr Gln Met Ala Glu Tyr Leu Val Asp Asp Val Ser Pro Tyr Phe
100 105 110
Leu His Met Glu Met Phe Ile Thr Thr Ile Arg Glu Gln Ala Ser Glu
115 120 125
Glu Gln Gln Ala His Trp Leu Pro Leu Ile Glu Leu Trp Lys Ile Ile
130 135 140
Gly Ala Tyr Ala Gln Thr Glu Leu Gly His Gly Ser Asn Val Arg Gly
145 150 155 160
Leu Glu Leu Glu Ala Arg Trp Asp Asn Arg Thr Lys Glu Phe Val Leu
165 170 175
His Ser Pro Thr Leu Thr Ala Ser Lys Trp Trp Asn Gly Ser Leu Gly
180 185 190
Arg Leu Ala Asn His Ala Ile Val Val Ala Gln Leu Leu Leu Pro Asp
195 200 205
Pro Ser Ser Pro Asp Gln Tyr Val Ser His Gly Pro His Pro Phe Ile
210 215 220
Val Gln Val Arg Asp Met Lys Thr His Gln Pro Leu Asn Gly Ile Val
225 230 235 240
Val Gly Asp Ile Gly Pro Lys Tyr Gly Tyr Ile Thr Met Asp Asn Ala
245 250 255
Tyr Met Leu Phe Asp Gln Phe Arg Ile Pro His Ser Ala Met Leu Ser
260 265 270
Arg Tyr Ser Lys Val Asp Leu Asn Thr Gly Ile Tyr Thr Lys Pro Glu
275 280 285
Lys Pro Ala Leu Val Tyr Gly Ser Leu Thr Tyr Val Arg Ser Asn Met
290 295 300
Val His Arg Ala Arg Leu Val Leu Ala Arg Ala Val Thr Val Ala Val
305 310 315 320
Arg Tyr Ser Ser Val Arg Arg Gln Phe Gln Asp Arg Asp Gly Asp Lys
325 330 335
Thr Gly Pro Glu Met Ser Val Leu Asp Tyr Pro Thr Val Gln Ile Arg
340 345 350
Ile Leu Pro Leu Leu Ala Thr Thr Phe Ala Leu His Tyr Thr Gly Leu
355 360 365
Ala Met Gln Thr Val Tyr Lys Asn Ala Arg Gln Asp Ile Glu Glu Gly
370 375 380
Asn Phe Asn Ser Leu Ala His Met His Ser Met Ser Ser Gly Leu Lys
385 390 395 400
Ser Leu Cys Thr Ile Phe Ala Ala Asp Gly Ile Glu Thr Cys Arg Arg
405 410 415
Ala Met Gly Gly His Gly Phe Gly Gly Gly Ser Gly Leu Ile Gln Val
420 425 430
Asn Asn Asp Tyr Leu Ser Lys Pro Thr Val Glu Gly Asp Asn Trp Met
435 440 445
Ile Thr Gln Gln Val Ala Ala Tyr Val Ile Lys Lys Met Thr Ala Ala
450 455 460
Val Gly Ser Pro Asp Thr Pro Gly Ile Asp Glu Thr Asp Ala Arg Phe
465 470 475 480
Lys Glu Phe Ile Arg Asn Lys Arg Arg Pro Glu Ser Glu Lys Arg Thr
485 490 495
Tyr Asp Ile Leu Asn Ser Asp Leu Asp Ile Val Lys Ser Phe Glu Leu
500 505 510
Arg Ala Thr Ala Met Ala Ser Ser Thr Lys Leu Ile Arg Val Ile Lys
515 520 525
Lys Arg Asn Trp Asn Ser Leu Leu Ile Gln Leu His Lys Leu Ser Arg
530 535 540
Ala Gln Ser Glu Ser Ile Ile Val Ala Thr Phe Phe Asp Ala Leu Ser
545 550 555 560
Asn Asp Lys Thr Leu Ser Ala Pro Thr Lys Asn Val Leu Trp Asp Cys
565 570 575
Tyr Arg Leu Phe Ala Leu Tyr Ser Met Glu Asn Glu Ser Phe Glu Phe
580 585 590
Leu Arg Thr Asn Ala Val Ser Gln Thr Asp Leu Asp Ser Leu Ala Ser
595 600 605
Arg Val Gln Asp Leu Met Ala Arg Ile Arg Pro His Ala Val Thr Leu
610 615 620
Val Asp Ser Trp Met Ile Pro Asp Tyr Leu Leu Asp Ser Ala Leu Gly
625 630 635 640
Arg Tyr Asp Gly Arg Val Tyr Glu Asp Leu Phe Asn Arg Ala His Arg
645 650 655
Leu Asn Pro Leu Asn Arg Ile Thr Phe Asn Pro Asn Tyr Trp Glu Asp
660 665 670
Glu Ile Val Lys Gly Ser Gly Asp Asn Gly Arg Gly Ile Leu Ser Lys
675 680 685
Leu
<210> 43
<211> 425
<212> PRT
<213> Penicillium chrysogenum
<400> 43
Met Ser Val Ser Asn Val Pro Phe Ala Asp Pro Leu Trp Leu Asn Arg
1 5 10 15
Lys His Ser Pro Tyr Tyr Lys Asp Ser His Arg Lys Leu Gln Lys Glu
20 25 30
Val Arg Gln Tyr Val Asp Glu His Ile Ser Pro Phe Cys Glu Glu Trp
35 40 45
Glu Lys Gln Gly Phe Val Pro Pro Glu Ala Gln Lys Arg His Ala Glu
50 55 60
Leu Gly Tyr Thr Ala Val Ala Ser Phe Pro Leu Ala Ala Asp Tyr Leu
65 70 75 80
Asp Gly Gln Arg Leu Pro Gly Asp Ile Asn Pro Tyr Glu Trp Asp Gly
85 90 95
Phe His Asp Ile Val Val Ile Asp Glu Leu Ala Arg Cys Gly Tyr Leu
100 105 110
Gly Ile Val Trp Ala Leu Gly Cys Gly Asn Ser Ile Gly Gly Pro Pro
115 120 125
Ile Ile Asn Phe Gly Asn Glu Glu Gln Lys Arg Arg Phe Leu Pro Asp
130 135 140
Met Leu Lys Gly Lys Ile Arg Phe Cys Leu Gly Val Thr Glu Pro Asp
145 150 155 160
Ala Gly Ser Asp Val Ala Gly Ile Thr Thr Val Ala Glu Arg Lys Gly
165 170 175
Asp Ala Tyr Ile Val Asn Gly Ala Lys Lys Trp Ile Thr Asn Gly Ile
180 185 190
Phe Ala Asp Phe Cys Thr Ala Ala Val Arg Thr Gly Gly Ser Gly Thr
195 200 205
His Gly Ile Ser Ala Leu Val Ile Pro Met Lys Ala Pro Gly Val Ile
210 215 220
Cys Arg Lys Ile Glu Asn Ser Gly Val His Ala Ser Gly Ser Thr Tyr
225 230 235 240
Ile Glu Phe Asp Gln Val Glu Val Pro Val Asp Asn Leu Leu Gly Glu
245 250 255
Glu Asn Lys Gly Phe Pro Val Ile Met Asn Asn Phe Asn His Glu Arg
260 265 270
Leu Trp Leu Ala Cys Thr Ser Leu Arg Met Ala Arg Val Cys Ala Glu
275 280 285
Asp Ala Tyr Gln His Ala Ile Thr Arg Glu Thr Phe Gly Lys Arg Leu
290 295 300
Ile Glu Asn Gln Ile Ile Arg Ser Lys Phe Ser Ala Met Ala Arg Ser
305 310 315 320
Leu Asp Ser Asn Tyr Ala Trp Met Glu Gln Leu Val Tyr Ile Ala Glu
325 330 335
Ile Ala Lys Lys Glu Gly Thr Asp Ala Gly Thr Gly Gly Leu Phe Ala
340 345 350
Asn Leu Lys Val Leu Ala Gly Gln Thr Leu Glu Lys Val Asn Arg Glu
355 360 365
Ser Gln Gln Val Met Gly Gly Leu Gly Tyr Ser Lys Asn Gly Arg Gly
370 375 380
Ser Arg Ile Glu Gln Val Ser Arg Asp Val Arg Val Met Val Val Gly
385 390 395 400
Gly Gly Ser Glu Glu Ile Leu Ser Glu Leu Ala Val Asn Gln Glu Ile
405 410 415
Lys Ala Met Lys Lys Gln Ser Lys Leu
420 425
<210> 44
<211> 605
<212> PRT
<213> Penicillium chrysogenum
<400> 44
Met Ala Ala Gln Ser Arg Val Pro Ala Thr Ala Glu Ser Gly Thr Phe
1 5 10 15
Thr Gln Leu Gly Pro Leu Pro Asn Thr Tyr Thr Ser Asp Ile Ser Leu
20 25 30
Gln Arg Met Leu Gly Trp Tyr Leu Pro Ala Gln Thr Leu Lys Leu Ile
35 40 45
Glu Pro His Leu Ala Gln Leu Gly Glu Glu Ala Val Ser Pro Gln Val
50 55 60
Phe Ala Trp Asn Ala Asp Ala Glu Thr Asn Leu Pro Tyr Val Lys Lys
65 70 75 80
Tyr Asn Val Trp Gly Gln Arg Tyr Ala Tyr Asp Arg Leu Val Thr Thr
85 90 95
Asp Gly Trp Lys Gln Leu Gly Lys Trp Gly Ala Lys His Gly Val Val
100 105 110
Ser Leu Gly Tyr Asp His Thr Tyr Gly Val Tyr Arg Arg Thr Ala Gln
115 120 125
Tyr Ala Ala Val Tyr Leu Tyr Ala Pro Ser Ser Ala Met Tyr Arg Cys
130 135 140
Pro Met Ser Met Ser Asp Gly Ala Ala Leu Thr Ser Gly Gln Trp Met
145 150 155 160
Thr Glu Arg Ala Gly Gly Ser Asp Val Gln Asn Thr Glu Thr Trp Ala
165 170 175
Thr Tyr Ala Pro Leu Pro Gln Glu Ser Lys Thr Ser Asp Val Leu Ala
180 185 190
Glu Gly Asp Tyr Leu Ile Ser Gly Phe Lys Phe Phe Ser Ser Ala Thr
195 200 205
Asp Ala Asn Val Ala Phe Leu Leu Ala Lys Thr Asp Ser Gly Lys Leu
210 215 220
Ser Thr Phe Ile Ala Pro Leu Arg Lys Thr Ser Ile Gly Ala Asp Gly
225 230 235 240
Lys Pro Glu Glu Thr Ser Asn Gly Val Arg Ile His Arg Phe Lys Asn
245 250 255
Lys Leu Gly Thr Lys Glu Leu Pro Thr Ala Glu Leu Glu Leu Lys Gly
260 265 270
Met Arg Ala His Leu Val Gly Glu Leu Asp Gln Gly Ile Val Thr Ile
275 280 285
Ala Pro Leu Leu Asn Thr Thr Arg Ile Gln Thr Leu Leu Gly Thr Leu
290 295 300
Ser Thr Trp Arg Arg Ala Ile Ser Ile Thr Lys Asn Phe Ala Lys Ser
305 310 315 320
Arg Thr Thr Val Gly Glu Pro Leu Trp Leu Ile Pro Met His Leu Arg
325 330 335
Leu Leu Ala Asp Val Glu Val Lys His Arg Gly Ala Ile Asn Leu Ala
340 345 350
Phe Phe Thr Ile Ala Val Met Gly Leu Ile Glu Asn Pro Ser Ser Pro
355 360 365
Ala Arg His Ala His Met Pro Arg Asp Pro Thr Glu Ala Lys Val Val
370 375 380
Phe Arg Val Leu Thr Ala Thr Ser Lys Gly Val Val Ser Lys Met Ser
385 390 395 400
Met Val Gly Val Gln Glu Cys Gln Glu Ala Ile Gly Gly Val Gly Tyr
405 410 415
Ile Asp Glu Pro Asp Glu Pro Glu Phe Asn Ile Ser Arg Leu Leu Arg
420 425 430
Ser Ala Ala Val Tyr Pro Ile Trp Glu Gly Thr Thr Asn Val Leu Ala
435 440 445
Ser Glu Leu Val Arg Phe Leu Met Lys Gly Asp Asn Leu Ser Ile Leu
450 455 460
Ser Gly Trp Leu Gly His Val Val Ser Leu Ile Arg Thr Pro Ser Leu
465 470 475 480
Ala Gly Ala Leu Lys Gln Ala Met Ala Ser Tyr Leu Ser Arg Val Thr
485 490 495
Ser Thr Arg Pro Gln Ala Ala Leu Leu Ala Asp Ala Arg Arg Val Met
500 505 510
Phe Thr Phe Ala Trp Ile Leu Ser Gly Ala Leu Leu Thr Leu Asp Ala
515 520 525
Glu Arg Asp Glu Asp Glu Val Ala Met Glu Ile Ala Arg Arg Trp Val
530 535 540
Leu Leu Gly Glu Gly Gly Val Gly Glu Phe Val Tyr Arg Asp Ile Ala
545 550 555 560
Lys Pro Tyr Gln Cys Phe Asn Leu Arg Ser Gly Arg Asp Glu His Thr
565 570 575
Arg Leu Asp Cys Lys Ile Ala Trp Gly Val Glu Leu Pro Gly Lys Ile
580 585 590
Val Phe Gly His Arg Ser Leu Ser Glu Ser Ser Lys Leu
595 600 605
<210> 45
<211> 425
<212> PRT
<213> Penicillium chrysogenum
<400> 45
Met Met Asn Arg Ser Leu Leu Arg Ala Ala Ala Arg Ser Ile Gln Ala
1 5 10 15
Gly Pro Thr Ser Val Ala Gly Arg Arg Tyr Ala Ser Ser Ala Val Phe
20 25 30
Asn Trp Glu Asp Pro Leu Ala Ala Ala Glu Leu Phe Thr Asp Glu Glu
35 40 45
Leu Ala Ile Gln Asp Thr Ala Arg Gln Tyr Cys Gln Asp Lys Leu Ala
50 55 60
Pro Arg Val Leu Glu Ala Tyr Arg Asn Glu Asp Tyr Asp Arg Arg Ile
65 70 75 80
Leu Glu Glu Met Gly Asp Leu Gly Leu Leu Gly Ala Ser Ile Glu Gly
85 90 95
Tyr Gly Cys Ala Gly Val Ser Thr Val Ala Ser Gly Leu Ile Thr Lys
100 105 110
Glu Val Glu Arg Val Asp Ser Gly Tyr Arg Ser Gly Met Ser Val Gln
115 120 125
Ser Ser Leu Ala Met Thr Gly Ile Tyr Glu Phe Gly Thr Glu Glu Gln
130 135 140
Lys Gln Arg Phe Leu Pro Ser Leu Ala Lys Gly Thr Leu Ala Gly Cys
145 150 155 160
Phe Gly Leu Thr Glu Pro Asn His Gly Ser Asp Pro Gly Ser Met Glu
165 170 175
Thr Val Ala Arg Glu His Pro Thr Gln Lys Gly Met Tyr Leu Leu Ser
180 185 190
Gly Ser Lys Thr Trp Ile Thr Asn Ser Pro Ile Ala Asp Val Ala Leu
195 200 205
Val Trp Ala Lys Leu Glu Ser Thr Gly Lys Ile Arg Gly Phe Ile Val
210 215 220
Glu Arg Glu Arg Ala Thr Pro Gly Ser Tyr Glu Thr Pro Ala Ile Lys
225 230 235 240
Asn Lys Ser Ala Leu Arg Ala Ser Ile Thr Gly Met Ile Gln Met Asp
245 250 255
Asn Cys Pro Val Pro Ala Glu Asn Met Leu Pro Glu Val Glu Gly Leu
260 265 270
Lys Gly Pro Phe Thr Cys Leu Asn Ser Ala Arg Leu Gly Ile Ala Phe
275 280 285
Gly Ala Met Gly Ala Leu Glu Asp Cys Leu Ala Arg Ala Arg Glu Tyr
290 295 300
Ser Leu Glu Arg Lys Gln Phe Lys Gly Asn Pro Leu Ala Lys Tyr Gln
305 310 315 320
Leu Ile Gln Met Lys Leu Ala Asn Ala Ala Thr Asp Ala Ala Tyr Gly
325 330 335
Thr Leu Ala Ala Val Gln Val Ala Arg Leu Lys Asp Ala Gly Lys Ala
340 345 350
Thr Pro Glu Met Ile Ser Met Ile Lys Arg Gln Asn Cys Asp Arg Ala
355 360 365
Leu Ala Asn Ser Arg Ile Leu Gln Glu Val Phe Gly Gly Asn Ala Ala
370 375 380
Ser Asp Glu Tyr His Ile Ala Arg His Val Ala Asn Leu Phe Val Val
385 390 395 400
Gln Thr Tyr Glu Gly Gln Ser Asp Ile His Ala Leu Ile Leu Gly Arg
405 410 415
Ala Ile Thr Gly Lys Gln Ala Phe Val
420 425
<210> 46
<211> 633
<212> PRT
<213> Penicillium chrysogenum
<400> 46
Met Ser Ser Ser Gln Pro Ala Thr Ala Asp Ser Gly Tyr Ile Thr Asp
1 5 10 15
Pro Gly Pro Leu Glu Asn Thr Tyr Thr Ser Asp Pro Ser Leu Gln Arg
20 25 30
Ala Leu Ala Trp Tyr Leu Pro Ser Ala Thr Leu Gln Ser Val Gln Pro
35 40 45
His Leu Thr Gln Phe Gly Ala Glu Ala Ile Ser Glu Gln Val Arg Glu
50 55 60
Trp Ser Ala Asp Ala Glu Arg Asn Val Pro Tyr Val Lys Ser His Asn
65 70 75 80
Val Trp Gly Lys Arg Tyr Asp Tyr Asp Arg Leu Val Thr Thr Glu Gly
85 90 95
Trp Lys Gln Leu Gly Lys Trp Gly Ala Arg Asn Arg Ile Val Ser Ala
100 105 110
Gly Tyr Asp Lys Ser Leu Gly Val Asp Arg Arg Thr Val Gln Tyr Ala
115 120 125
Leu Asn Tyr Leu Tyr Ser Pro Ser Ser Gly Leu Tyr Ser Cys Pro Ile
130 135 140
Ser Met Thr Asp Gly Ala Ala Phe Ile Leu Ser Ser Arg Ile Asn Lys
145 150 155 160
Leu Pro Ser Thr His Pro Phe His Thr Ala Phe Gln Gly Leu Ile Ser
165 170 175
Glu Lys Asp Asp His Trp Thr Ser Gly Gln Trp Met Thr Glu Arg Ala
180 185 190
Gly Gly Ser Asp Val Gln Asn Thr Glu Thr Trp Ala Thr Tyr Ser Pro
195 200 205
Leu Ala Ser Ser Ser Gly Ser Glu Pro Leu Gly Asp Gly Asp Tyr Leu
210 215 220
Ile Ser Gly Phe Lys Phe Phe Ser Ser Ala Thr Asp Ala Asn Leu Ala
225 230 235 240
Leu Leu Leu Ala Lys Thr Pro Ser Gly Lys Leu Ser Thr Phe Leu Ala
245 250 255
Pro Leu Arg Arg Thr Val Val Gly Gly Asp Gly Val Ser Arg Val Val
260 265 270
Ser Asn Gly Val Arg Ile His Arg Leu Lys Asn Lys Leu Gly Thr Lys
275 280 285
Glu Leu Pro Thr Ala Glu Leu Glu Leu Lys Asp Met Arg Ala His Leu
290 295 300
Ile Gly Glu Ile Asp Gln Gly Ile Val Thr Ile Ala Pro Leu Leu Asn
305 310 315 320
Val Thr Arg Leu His Thr Phe Val Gly Ser Leu Ala Gly Trp Arg Arg
325 330 335
Ala Ile Ser Ile Thr Lys Ser Phe Ala Lys Ala Arg Thr Thr Val Gly
340 345 350
Glu Pro Leu Trp Leu Ile Pro Met His Leu Arg Leu Leu Ala Asp Met
355 360 365
Glu Val Lys His Arg Gly Ala Met Asn Leu Ala Trp Phe Thr Val Ala
370 375 380
Leu Phe Gly Val Val Glu Asp Arg Thr Pro Ser Ser Asn Lys Ile Ala
385 390 395 400
His Leu Pro Gln Pro Gly Lys Glu Ala Glu Val Val Phe Arg Thr Leu
405 410 415
Thr Ala Thr Ala Lys Ala Val Ile Ser Lys Met Ala Thr Ala Gly Ile
420 425 430
Gln Glu Cys Gln Glu Ser Met Gly Gly Val Gly Tyr Met Asp Glu Ala
435 440 445
Asp Glu Pro Glu Phe Asn Ile Ser Arg Ile Leu Arg Asn Asn Ala Val
450 455 460
Asn Ser Ile Trp Glu Gly Thr Thr Asn Val Leu Ala Ser Glu Phe Val
465 470 475 480
Arg Phe Leu Ile Lys Lys Asp Asn Leu Lys Ile Phe Gly Thr Trp Leu
485 490 495
Asp Arg Thr Leu Ala Leu Ile Gln Ser Val Asp Leu Arg Asn Ala Leu
500 505 510
Thr Ala Ala Trp Leu Ala Leu His Ala Arg Phe Met Thr Gln Asp Pro
515 520 525
Ala Thr Thr Val Ala Asp Gly Arg Arg Phe Met Phe Thr Val Ala Trp
530 535 540
Ile Leu Ser Gly Ala Leu Leu Ala Leu Asp Thr Glu Arg Asp Asn Asp
545 550 555 560
Pro Val Thr Ala Glu Ile Ala Arg Arg Trp Ile Leu Ser Ala Glu Gly
565 570 575
Gly Val Gly Glu Gln Val Phe His Asp Ile Val Thr Val Ser Gly Thr
580 585 590
Ala Ser Ala Thr Ser Gly Gly Glu Glu His Leu Gln Trp Asp Cys Arg
595 600 605
Ile Ala Trp Gly Val Asp Leu Pro Ala Asn Arg Ala Ser Gly His Arg
610 615 620
Ser Leu Gln Lys Ala Gly Ser Lys Leu
625 630
<210> 47
<211> 695
<212> PRT
<213> Penicillium chrysogenum
<400> 47
Met Pro Ser Pro Pro Pro Asp Trp Val Lys Ala Leu Lys Pro Ser Gly
1 5 10 15
Pro Gln Gly Ser Glu Leu Leu His Gln Glu Arg Ala Gln Ser Asn Val
20 25 30
Asp Val Glu Arg Leu Ser Glu Leu Leu His Thr Lys Glu Thr Leu Glu
35 40 45
Arg Arg Ala Ser Leu Leu Ala Leu Leu Gln Pro Glu Lys Val Phe Asp
50 55 60
Lys Ser Gln Asn His Ser Leu Gly Arg Val Glu Arg Leu Gln Arg Ser
65 70 75 80
Leu Ala Lys Ala Lys Arg Leu Gln Gln Leu Ala Glu Glu His Lys Trp
85 90 95
Ser Met Gln Glu Leu His Ala Ala Asn Asp Leu Ile Gly Glu Pro Thr
100 105 110
Pro Tyr Gly Leu His Ala Ser Met Phe Leu Val Thr Leu Arg Glu Gln
115 120 125
Gly Thr Pro Glu Gln His Lys Leu Phe Leu Glu Arg Ala Glu Lys Tyr
130 135 140
Glu Ile Ile Gly Cys Tyr Ala Gln Thr Glu Leu Gly His Gly Ser Asn
145 150 155 160
Val Arg Gly Leu Glu Thr Thr Ala Thr Trp Asn Ser Asn Asp Lys Thr
165 170 175
Phe Thr Ile Asn Ser Pro Thr Leu Thr Ala Ser Lys Trp Trp Ile Gly
180 185 190
Ser Leu Gly Arg Thr Ala Asn His Ala Val Val Met Ala Gln Leu Phe
195 200 205
Ile Asp Gly Lys Asn Tyr Gly Pro His Pro Phe Val Val Gln Val Arg
210 215 220
Asp Leu Glu Thr His Gln Pro Leu Asp Asn Val Tyr Val Gly Asp Ile
225 230 235 240
Gly Pro Lys Phe Gly Tyr Asn Thr Met Asp Asn Gly Phe Leu Leu Phe
245 250 255
Asn Asn Val Lys Ile Pro His Val Asn Met Leu Ala Arg Phe Cys Gln
260 265 270
Val Asp Lys Glu Thr Asn Gln Tyr Ala Lys Pro Ala Met Pro Ser Leu
275 280 285
Val Phe Gly Thr Met Thr Trp Val Arg Ala Asn Ile Val Leu Asp Ala
290 295 300
Gly Gly Val Leu Ala Arg Gly Val Thr Ile Ala Thr Arg Tyr Cys Ala
305 310 315 320
Val Arg Arg Gln Phe Gln Asp Arg Asp Ala Asp Pro His Ala Gly Glu
325 330 335
Thr Gln Val Leu Asn Tyr Lys Met Val Gln Val Arg Leu Leu Pro Leu
340 345 350
Leu Ala Ser Met Tyr Ala Leu His Phe Thr Gly Arg Gly Met Met Arg
355 360 365
Leu Tyr Glu Glu Asn Gln Ser Arg Met Lys Ala Ala Ser Ser Pro Asp
370 375 380
Gln Glu Ser Arg Gly Ala Gly Pro Glu Gln Leu Arg Ala Gly Ala Asn
385 390 395 400
Leu Leu Ala Asp Leu His Ala Thr Ser Cys Gly Leu Lys Ala Leu Ala
405 410 415
Ser Thr Thr Ala Gly Glu Gly Leu Glu Ile Cys Arg Arg Ala Cys Gly
420 425 430
Gly His Gly Tyr Ser Ser Tyr Ser Gly Ile Gly Pro Ala Tyr Ala Asp
435 440 445
Tyr Leu Pro Thr Leu Thr Trp Glu Gly Asp Asn Tyr Met Leu Thr Gln
450 455 460
Gln Val Ala Arg Tyr Leu Leu Lys Ser Ala Arg Ala Val Leu Ala Gly
465 470 475 480
Lys Pro Ala Arg Asn Asp Thr Ser Gln Ile Leu Gln Ala Tyr Leu Asp
485 490 495
Arg Arg Asp Lys Gly Ala Ser Phe Asp Val Leu Asp Glu Asp Lys Asp
500 505 510
Ile Val Ala Ala Phe Gly Trp Arg Thr Ala His Leu Thr Phe Glu Ala
515 520 525
Leu Lys His Arg Asp Ala Glu Gln Arg Ser Trp Asn Ser Leu Leu Val
530 535 540
Asp Phe Trp Arg Leu Ser Thr Ala His Ser Gln Tyr Leu Met Val Lys
545 550 555 560
Asn Phe Tyr Glu Ala Val Ser Ser Pro Glu Leu Ser Gly Ala Leu Asp
565 570 575
Pro Glu Thr Lys Gly Leu Met His Gln Leu Phe Arg Leu Phe Ser Leu
580 585 590
His Thr Leu Glu Arg Glu Ala Ala Glu Phe Phe Ser Ser Gly Ala Val
595 600 605
Thr Val Arg Gln Ile Thr Leu Thr Arg Thr Thr Ala Val Leu Lys Leu
610 615 620
Leu Asp Asp Ile Arg Pro His Ala Val Arg Leu Val Asp Ala Trp Ala
625 630 635 640
Ile Pro Asp Trp Gln Leu Asp Ser Ser Leu Gly Arg Tyr Asp Gly Lys
645 650 655
Val Tyr Glu Asp Leu Phe Arg Arg Ala Ser Glu Glu Asn Pro Val Asn
660 665 670
Glu Leu Val Phe Asp Pro Tyr Pro Trp Asn Ser Ala Leu Leu Lys Asn
675 680 685
Glu Pro Ala Lys Ser Lys Leu
690 695
<210> 48
<211> 542
<212> PRT
<213> Penicillium chrysogenum
<400> 48
Met Ala Lys Thr Phe Ser Lys Glu Asp Val Ala Ser His Ser Lys Gly
1 5 10 15
Asp Ser Pro Trp Ile Ile Ile Asp Glu Asp Val Tyr Asp Val Ser Lys
20 25 30
Phe Gln Glu Glu His Pro Val Leu Gln Arg Val Ala Gly Lys Asp Ala
35 40 45
Ser Lys Gln Phe Trp Lys Tyr His Asn Glu Gly Ile Leu Lys Lys Tyr
50 55 60
Lys Gly Gln Leu Gln Ile Gly Ser Leu Asp Thr Lys Lys Ala Ala Pro
65 70 75 80
Ala Pro Pro Thr Pro Ala Pro Ala Pro Lys Lys Ala Ala Pro Glu Pro
85 90 95
Lys Ser Thr Ala Ser Ser Ser Ser Val Thr Pro Pro Ala Thr Gly Ala
100 105 110
Pro Gln Asp Pro Tyr Gly Glu Leu Ile Pro Phe Ala Asp Pro Ser Trp
115 120 125
Tyr Gln Gly Tyr Ala Ser Pro Tyr Phe Asn Glu Ser His Ala Ala Leu
130 135 140
Arg Asp Glu Val Arg Gln Trp Val Glu Ser Glu Ile Glu Pro Tyr Val
145 150 155 160
Thr Glu Trp Asp Glu Ala Lys Glu Val Pro Ala His Ile Tyr Lys Gln
165 170 175
Met Gly Glu Arg Gly Tyr Leu Ala Gly Leu Leu Gly Val His Phe Pro
180 185 190
Glu Lys His Thr Pro His Arg Val Lys Ser Val Ser Pro Asp Arg Trp
195 200 205
Asp Leu Phe His Glu Met Leu Leu Thr Asp Glu Leu Ser Arg Ala Gly
210 215 220
Ser Gly Gly Leu Val Trp Ser Leu Ile Gly Gly Tyr Gly Ile Gly Cys
225 230 235 240
Pro Pro Leu Val Lys Phe Gly Lys Lys Pro Leu Val Asp Arg Ile Leu
245 250 255
Pro Gly Ile Leu Ala Gly Asp Lys Arg Ile Cys Leu Ala Ile Thr Glu
260 265 270
Pro Asp Ala Gly Ser Asp Val Ala Asn Leu Gly Cys Glu Ala Lys Leu
275 280 285
Thr Glu Asp Gly Lys His Tyr Ile Val Asn Gly Glu Lys Lys Trp Ile
290 295 300
Thr Asn Gly Ile Tyr Ser Asp Tyr Phe Thr Thr Ala Val Arg Thr Gly
305 310 315 320
Lys Asp Gly Met Asn Gly Leu Ser Val Leu Leu Ile Glu Arg Glu Ala
325 330 335
Gly Gly Val Ser Thr Arg Arg Met Asp Cys Gln Gly Val Trp Ser Ser
340 345 350
Gly Thr Thr Tyr Val Thr Phe Glu Asp Val Lys Val Pro Val Glu Asn
355 360 365
Leu Ile Gly Lys Glu Asn Gln Gly Phe Lys Val Ile Met Thr Asn Phe
370 375 380
Asn His Glu Arg Ile Gly Ile Val Ile Gln Cys Val Arg Phe Ser Arg
385 390 395 400
Val Cys Tyr Glu Glu Ser Met Lys Tyr Ala His Lys Arg Lys Thr Phe
405 410 415
Gly Lys Arg Leu Ile Asp His Pro Val Ile Arg Met Lys Leu Ala His
420 425 430
Met Ala Arg Gln Ile Glu Ala Thr Tyr Asn Trp Leu Glu Asn Ile Ile
435 440 445
Phe Gln Cys Gln Ser Met Glu Asp Thr Glu Ala Met Leu Lys Leu Gly
450 455 460
Gly Ala Ile Ala Gly Leu Lys Ala Gln Ser Thr Gln Cys Phe Glu Phe
465 470 475 480
Cys Ala Arg Glu Ala Ser Gln Ile Phe Gly Gly Leu Ser Tyr Ser Arg
485 490 495
Gly Gly Gln Gly Gly Lys Ile Glu Arg Leu Tyr Arg Asp Val Arg Ala
500 505 510
Tyr Ala Ile Pro Gly Gly Ser Glu Glu Ile Met Leu Asp Leu Ser Met
515 520 525
Arg Gln Ser Leu Arg Val His Gly Met Phe Gly Met Lys Leu
530 535 540
<210> 49
<211> 438
<212> PRT
<213> Penicillium chrysogenum
<400> 49
Met Ala Asp Thr Leu Arg Pro Ala Thr Ala Pro Tyr Ser Glu Pro Leu
1 5 10 15
Leu Pro Gln Leu Asp Val Arg Asn Pro Tyr Tyr Thr Asp Leu His His
20 25 30
Asn Leu Arg Ala Thr Val Arg Glu Tyr Val Asp Thr Tyr Ile Ser Pro
35 40 45
Tyr Ala Ala Glu Trp Glu Glu Ala Gly Gln Val Pro Glu Ala Val Arg
50 55 60
Arg Arg His Cys Lys Leu Gly Tyr Ser Ile Val His Pro Leu Thr Ser
65 70 75 80
Glu Glu Asp Ser Ala Gly Ile Ser Leu Pro Gly Asn Val Pro Arg Glu
85 90 95
Lys Trp Asp Thr Trp Cys Ser Leu Ile Val Ser Asp Glu Leu Thr Arg
100 105 110
Val Gly Tyr Val Gly Val Ile Trp Gly Leu Gly Gly Gly Asn Gly Ile
115 120 125
Gly Cys Pro Pro Val Ala Arg Phe Gly Asn Ala Glu Gln Arg Lys Lys
130 135 140
Trp Leu Pro Gly Val Ala Arg Gly Asp Ile Arg Phe Cys Leu Gly Ile
145 150 155 160
Thr Glu Pro Asp Ala Gly Ser Asp Val Ala Asn Ile Gln Thr Thr Ala
165 170 175
Gln Arg Asp Gly Asn His Tyr Val Val Asn Gly Ser Lys Lys Trp Ile
180 185 190
Thr Asn Gly Ile Trp Ala Asp Tyr Cys Thr Ala Ala Val Arg Thr Gly
195 200 205
Gly Pro Gly Arg Ser Gly Ile Ser Leu Leu Val Ile Pro Leu Ala Thr
210 215 220
Ala Gly Val Thr Arg Arg Arg Met His Asn Ser Gly Val Asn Ala Ser
225 230 235 240
Gly Ser Thr Phe Ile Glu Phe Glu Asp Val Arg Val Pro Val Glu Asn
245 250 255
Leu Val Gly Gln Glu Asn Lys Gly Phe Pro Leu Ile Met Ser Asn Phe
260 265 270
Asn Pro Glu Arg Leu Ala Leu Ala Cys Ala Ser Leu Arg Leu Ala Arg
275 280 285
Val Cys Ala Glu Asp Ala Tyr Asn Tyr Ala Ile Lys Arg Glu Thr Phe
290 295 300
Gly Ser Ala Leu Ile Glu Lys Gln Ala Ile Gln Ser Lys Ile Phe Lys
305 310 315 320
Phe Gly Leu Met Ile Glu Pro Ala Tyr Ala Phe Met Glu Gln Leu Val
325 330 335
Asn Ile Leu Glu Leu Thr Lys Asp Arg Pro Ser Asp Asp Val Asn Ile
340 345 350
Gly Gly Met Thr Ala Leu Leu Lys Val Met Ser Thr Arg Ala Leu Glu
355 360 365
Lys Ser Val Arg Glu Ala Gln Gln Ile Met Gly Gly Ala Gly Tyr Asn
370 375 380
Lys Ala Gly Lys Gly Ala Arg Ile Glu Gln Ile Ser Arg Asp Ala Arg
385 390 395 400
Val His Val Val Gly Gly Gly Ser Glu Glu Ile Met Ala Gly Leu Ala
405 410 415
Leu Arg Glu Glu Thr Lys Ala Ile Arg Thr Arg Arg Lys Ala Leu Glu
420 425 430
Lys Arg Gln Ser Lys Val
435
<210> 50
<211> 453
<212> PRT
<213> Penicillium chrysogenum
<400> 50
Met Asn Phe Asp Leu Pro Ala Asp Leu Lys Thr His Leu Glu Ser Ile
1 5 10 15
Asp Ser Phe Ile His Ser Thr Ile Leu Pro Leu Gln His Ser Asn Asp
20 25 30
Asn Asn Arg Phe Phe Asp His Arg Arg Glu Tyr Glu Arg Thr Asp Trp
35 40 45
Glu Asn Asn Gly Asn Pro Arg Lys Glu Trp Glu Glu Leu Leu Gly Glu
50 55 60
Ala Arg Thr Leu Ala Asp Ser Ser Gly Leu Tyr Arg Phe Ala Leu Pro
65 70 75 80
Arg Val Tyr Gly Gly Gln Ser His Pro Asp Val Asn Leu Trp Met Ser
85 90 95
Ala Ile Arg Tyr His Leu Ser Ser Asn Ala Val Tyr Gly Gly Gly Leu
100 105 110
Gly Leu Ala Asn Asp Leu Gln Asn Glu His Cys Ile Val Gly Asn Phe
115 120 125
Pro Asp Val Leu Met Leu His His Phe Gly Asn Glu Gln Gln Arg Asn
130 135 140
Thr Leu Ile Pro Ala Arg Leu Arg Gly Glu Phe Arg Thr Thr Phe Gly
145 150 155 160
Leu Thr Glu Pro Asp His Gly Ser Asp Ala Thr Phe Met Ser Thr Thr
165 170 175
Ala Arg Pro Thr Arg Gly Gly Phe Glu Ile Lys Gly Ala Lys Lys Trp
180 185 190
Gln Thr Gly Ala His His Cys Thr His Phe Leu Ile Phe Ala Arg Thr
195 200 205
Ser Gly Lys Ala Gly Ser Ala Gln Gly Ile Thr Ala Phe Leu Val Pro
210 215 220
Arg Asp Thr Arg Gly Val Arg Ile Val Ser Tyr Glu Trp Thr Leu Asn
225 230 235 240
Met Pro Thr Asp His Ala Thr Val Glu Leu Asn Ser Val Trp Val Pro
245 250 255
Glu Ser Ala Val Leu Gly Ser Ile Asp Gln Gly Leu Ala Ile Ala Gln
260 265 270
Thr Phe Val His Glu Asn Arg Ile Arg Gln Ala Ala Ser Ser Cys Gly
275 280 285
Ala Ala Arg Tyr Cys Leu Asp Arg Ser Ile Asp Arg Ala Arg Ala Arg
290 295 300
Lys Ile Trp Gly Glu Gly Lys Ser Leu Ala Asp Asn Gln Ala Ile Gln
305 310 315 320
Phe Pro Val Val Glu Leu Met Thr Gln Val Glu Met Leu Arg Leu Phe
325 330 335
Ile Leu Lys Thr Ser Trp Glu Met Asp Arg Ile Val Ala Glu Cys Gln
340 345 350
Ser Ser Lys Ala Gln Arg Ala Pro Trp Val Glu Ile Glu Gly Arg Leu
355 360 365
Ser Asp Gln Val Ala Met Cys Asn Phe Trp Ala Asn Arg Leu Cys Cys
370 375 380
Gln Ala Ala Asp Arg Ala Ile Gln Ile His Gly Gly Asp Gly Tyr Ser
385 390 395 400
Arg His Tyr Pro Phe Glu His Ile Tyr Arg His Phe Arg Arg Tyr Arg
405 410 415
Ile Thr Glu Gly Ala Glu Glu Ile Gln Met Arg Lys Ile Gly Ala Tyr
420 425 430
Ile Phe Gly Phe Ala Gly Pro Lys Lys Arg Glu Met Lys His Glu His
435 440 445
Ser Lys Ala Arg Ile
450
<210> 51
<211> 441
<212> PRT
<213> Penicillium chrysogenum
<400> 51
Met Ala Tyr Asn Ser Pro Asn Pro Ile Pro Phe Ser Glu Pro Pro Tyr
1 5 10 15
Ile Arg Gly Leu Pro Ser Pro Tyr Ile Thr Pro Ala His Arg Arg Phe
20 25 30
Gln Gln Ala Cys Arg Lys Phe Ala Thr Glu Asn Leu Ile Gln His Ala
35 40 45
Leu Glu Trp Glu Arg Glu Gly Thr Val Pro Glu His Val Phe His Thr
50 55 60
Phe Cys Lys His Asn Met Leu Leu Pro Asn Met Pro Ala Pro Leu Pro
65 70 75 80
Val Asp Trp Leu Lys Arg Leu Gly Ile Asn Asp Ile Leu Gly Val Lys
85 90 95
Val Glu Asp Trp Asp Tyr Ile Tyr Thr Gly Ile Tyr Cys Asp Glu Met
100 105 110
Ala Arg Ser Gly Leu Ser Gly Pro Ser Gly Ser Leu Asn Ala Gly Phe
115 120 125
Ala Phe Gly Ile Ala Pro Ile Tyr Lys Phe Gly Ser Thr Glu Leu Gln
130 135 140
Glu Arg Phe Leu Pro Glu Leu Leu Thr Gly Lys Lys Arg Gly Cys Ile
145 150 155 160
Ala Ile Thr Glu Pro Glu Ala Gly Ser Asp Val Ala Asn Ile Thr Thr
165 170 175
Thr Ala Val Lys Ser Ala Asp Gly Gln His Tyr Ile Leu Asn Gly Ser
180 185 190
Lys Lys Trp Ile Thr Asn Gly Ile Trp Ser Asp Tyr Ala Thr Met Ala
195 200 205
Val Arg Thr Gly Gly Pro Gly Ala Ala Gly Leu Ser Val Leu Val Val
210 215 220
Pro Leu Lys Gly His Pro Gly Val Ser Met Arg Arg Leu Lys Val Ser
225 230 235 240
Gly Gln Ile Thr Gly Gly Thr Thr Tyr Ile Glu Leu Asp Asp Val Lys
245 250 255
Val Pro Val Ser Asn Ile Ile Gly Lys Glu Gly Asp Gly Met Arg Ile
260 265 270
Ile Met Thr Asn Phe Asn His Glu Arg Leu Val Ile Ala Val Gly Val
275 280 285
Thr Arg Gln Ala Arg Val Ala Leu Ser Ala Ala Phe Ser Tyr Cys Leu
290 295 300
Lys Arg Glu Ala Phe Gly Lys Thr Leu Met Asp Gln Pro Val Val Arg
305 310 315 320
His Arg Leu Ala Lys Ala Gly Ala Glu Leu Glu Ser Met Trp Ala Trp
325 330 335
Val Glu Gln Ile Leu Tyr Gln Leu Val His Leu Ser Lys Glu Glu Gly
340 345 350
Asp Arg Gln Leu Gly Gly Leu Thr Ala Leu Ala Lys Ala Lys Ser Ala
355 360 365
Met Val Leu Asn Glu Cys Ala Gln Thr Ala Val Leu Leu Phe Gly Gly
370 375 380
Asn Gly Phe Thr Lys Thr Gly Gln Gly Glu Leu Val Glu Ala Ile Leu
385 390 395 400
Arg Asp Val Pro Gly Ala Arg Ile Pro Gly Gly Ser Glu Asp Val Leu
405 410 415
Leu Asp Leu Ser Val Arg Gln Leu Val Lys Leu Tyr Gln Ala Glu Glu
420 425 430
Lys Lys Leu Ser Lys Asn Ala Lys Ile
435 440
<210> 52
<211> 439
<212> PRT
<213> Penicillium chrysogenum
<400> 52
Met Tyr Arg Ser Gln Ile Gln Arg Ala Leu Arg Ser Gln Trp Pro Ala
1 5 10 15
Leu Arg Gln Leu Gln Cys Arg Thr Gly Leu Ser Pro Arg Ala Arg Ala
20 25 30
Ala Phe Ser Thr Ser Gly Arg Arg Asp Ile Met Gly Met Thr Gly Phe
35 40 45
Thr Asp Glu Gln Leu Thr Val Arg Glu Ala Ile Ser His Ile Cys Ser
50 55 60
Arg Phe Pro Asn Thr Tyr Trp Gln Glu Arg Asp Gln Gln Glu Lys Asp
65 70 75 80
Pro Lys Glu Phe His Ala Ala Leu Ala Lys Asp Gly Trp Leu Gly Ile
85 90 95
Ala Leu Pro Glu Ser Leu Gly Gly Ala Gly Leu Gly Ile Ser Glu Ala
100 105 110
Thr Met Met Met Gln Thr Ile Thr Gln Ser Gly Ala Gly Met Ala Gly
115 120 125
Ala Gln Ala Ile His Ala Asn Val Tyr Ala Thr Gln Pro Leu Ala Lys
130 135 140
Phe Gly Thr Lys Glu Gln Leu Glu Thr Thr Ile Pro Asn Ile Ile Asn
145 150 155 160
Gly Thr Trp Arg Thr Cys Phe Gly Val Thr Glu Pro Asn Thr Gly Leu
165 170 175
Glu Thr Leu Lys Leu Thr Thr Leu Ala Ser Lys Thr Asp Asp Gly Tyr
180 185 190
Ser Val Thr Gly Gln Lys Ile Trp Ile Thr Cys Ala Gln Val Ala Ser
195 200 205
Lys Met Ile Leu Leu Ala Arg Thr Thr Pro Leu Glu Glu Val Lys Lys
210 215 220
Ser Ser Glu Gly Leu Ser Leu Phe Cys Ile Asp Ile Asp Arg Glu Asn
225 230 235 240
Pro Gly Leu Asp Leu Arg Lys Ile Lys Lys Met Gly Gly Arg Ala Val
245 250 255
Asp Ala Asn Glu Val Phe Phe Asp His Tyr Lys Ile Pro Ala Asn Thr
260 265 270
Leu Ile Gly Glu Glu Asn Gln Gly Phe Lys Ile Ile Leu His Gly Met
275 280 285
Asn Ala Glu Arg Cys Leu Leu Ala Gly Glu Ala Leu Gly Leu Gly Tyr
290 295 300
Ala Ala Leu Glu Lys Ala Ser Gln Tyr Ala Lys Asp Arg Val Val Phe
305 310 315 320
Gly Arg Pro Ile Gly Gln Asn Gln Gly Val Ala His Pro Leu Ala Asp
325 330 335
Ala Phe Met Lys Leu Glu Ala Ala Lys Leu Ala Thr Tyr His Ala Ala
340 345 350
Arg Leu Tyr Asp Thr Asn Asp Gly Ser Val Pro Phe His Glu Ile Gly
355 360 365
Val Ala Cys Asn Ser Ala Lys Tyr Leu Ala Ala Glu Ala Ala Phe Thr
370 375 380
Ala Cys Glu Arg Ala Val Leu Ala His Gly Gly Met Gly Tyr Ala Val
385 390 395 400
Glu Tyr Asp Val Glu Arg Tyr Met Arg Glu Cys Phe Val Pro Arg Ile
405 410 415
Ala Pro Val Ser Arg Glu Met Ile Leu Asn Tyr Val Ser Glu Lys Val
420 425 430
Leu Asp Leu Pro Arg Ser Tyr
435
<210> 53
<211> 446
<212> PRT
<213> Penicillium chrysogenum
<400> 53
Met Ser Ala Ser Ser Arg Ile Pro Pro Ile Ala Gln Pro Phe Val Ser
1 5 10 15
Glu His Ala Lys Arg Thr Leu Asp Leu Val Glu Glu Phe Val Glu Lys
20 25 30
Asp Cys Ile Pro Ala Asp Thr Val Phe Ser Ala Gln Leu Gly Glu Gly
35 40 45
Glu Lys Arg Trp Thr Thr Thr Pro Thr Val Leu Glu Gly Leu Lys Glu
50 55 60
Lys Ala Lys Lys Leu Gly Leu Trp Asn Met Phe Leu Pro Lys Asn His
65 70 75 80
Phe Thr Gln Gly Ala Gly Phe Ser Asn Leu Glu Tyr Gly Leu Met Ala
85 90 95
Glu Leu Leu Gly Lys Ser Lys Val Ala Ser Glu Ala Thr Asn Asn Ala
100 105 110
Ala Pro Asp Thr Gly Asn Met Glu Val Phe Ala Lys Tyr Gly Asn Asp
115 120 125
Ala Gln Lys Lys Gln Trp Leu Ala Pro Leu Leu Glu Gly Lys Ile Arg
130 135 140
Ser Ala Phe Leu Met Thr Glu Pro Asp Val Ala Ser Ser Asp Ala Thr
145 150 155 160
Asn Ile Glu Leu Asn Ile Arg Arg Glu Gly Asn Glu Tyr Val Leu Asn
165 170 175
Gly Ser Lys Trp Trp Ser Ser Gly Ala Gly Asp Pro Arg Cys Ala Ile
180 185 190
Tyr Leu Val Met Gly Lys Thr Asp Pro Thr Asn Pro Asp Thr Tyr Lys
195 200 205
Gln Gln Ser Val Ile Leu Val Pro Ala Gly Leu Pro Gly Ile Thr Val
210 215 220
His Arg Met Leu Thr Val Tyr Gly Tyr Asp Asp Ala Pro His Gly His
225 230 235 240
Gly His Ile Thr Phe Lys Asp Val Arg Val Pro Ala Ser Asn Met Val
245 250 255
Leu Gly Glu Gly Arg Gly Phe Glu Ile Ile Gln Gly Arg Leu Gly Pro
260 265 270
Gly Arg Ile His His Ala Met Arg Ala Ile Gly Ala Ala Glu Arg Ala
275 280 285
Leu Glu Trp Leu Ile Ala Arg Val Asn Asp Glu Arg Lys Met Thr Phe
290 295 300
Gly Lys Pro Leu Val Ala His Gly Val Ile Leu Glu Trp Ile Ala Lys
305 310 315 320
Ser Arg Ile Glu Val Asp Ala Ala Arg Leu Ile Val Leu Asn Ala Ala
325 330 335
Ile Lys Ile Asp Gln Gly Asp Ala Lys Ser Ala Leu Lys Glu Ile Ala
340 345 350
Gln Ala Lys Val Leu Val Pro Gln Thr Ala Leu Thr Ile Ile Asp Arg
355 360 365
Ala Val Gln Ala Tyr Gly Ala Ala Gly Val Cys Gln Asp Thr Pro Leu
370 375 380
Ala Tyr Leu Trp Ala Gly Ile Arg Thr Leu Arg Ile Ala Asp Gly Pro
385 390 395 400
Asp Glu Val His Leu Gln Gln Leu Gly Lys Arg Glu Asn Lys Ala Arg
405 410 415
Lys Asp Ala Val Thr Ala Lys Leu Asn Trp Gln Arg Glu Glu Ala Asp
420 425 430
Arg Leu Leu Ala Ala Ser Gly Phe Lys Pro Lys Ser His Leu
435 440 445
<210> 54
<211> 513
<212> PRT
<213> Penicillium chrysogenum
<400> 54
Met Pro Ser Glu Thr Leu Thr Arg Ala Glu Val Ala Lys His Asn Thr
1 5 10 15
Glu Asp Ser Leu Trp Cys Ile Ile Asp His Gln Val Tyr Asp Leu Thr
20 25 30
Asp Phe Val Asp Ala His Pro Gly Gly Gly Val Val Leu Ala Gln Val
35 40 45
Ala Gly Lys Asp Ala Thr Ser Asp Phe Tyr Asn Leu His Arg Gln Glu
50 55 60
Val Leu Glu Lys Tyr Arg Asp Gln Leu Cys Ile Gly Val Val Glu Gly
65 70 75 80
Glu Lys Pro Glu Val Ile Arg Pro Phe Pro Gly Ala Leu Ser Pro Val
85 90 95
Pro Tyr Ala Glu Pro Leu Trp Leu Arg Pro Gln Phe Lys Ser Pro Tyr
100 105 110
Tyr Lys Glu Thr His Arg Asn Leu Gln Lys Ala Ile Arg Glu Phe Thr
115 120 125
Asp Lys Tyr Val Thr Pro Glu Ala Gln Glu Lys Glu Gln Asp Gly Ser
130 135 140
Tyr Ile Ser Gln Glu Leu Ile Asn Arg Met Ala Glu Thr Asn Ile Leu
145 150 155 160
Ala Met Arg Leu Gly Pro Gly Lys His Leu His Gly Arg Thr Leu Leu
165 170 175
Gly Gly Val Val Asp Gly Lys Glu Phe Asp Tyr Leu His Asp Met Ile
180 185 190
Ile Val Gln Glu Met Val Arg Ala Asn Ala Arg Gly Phe Gln Asp Gly
195 200 205
Asn Met Ala Gly Met Ala Ile Ser Leu Thr Ala Val Gln Gln Trp Leu
210 215 220
His Asp Pro Val Leu Lys Glu Arg Leu Asn Asp Glu Val Leu Ser Gly
225 230 235 240
Arg Lys Lys Met Cys Leu Ala Ile Thr Glu Ala Phe Ala Gly Ser Asp
245 250 255
Val Ala Gly Leu Lys Thr Thr Ala Glu Lys Thr Pro Asp Gly Lys His
260 265 270
Tyr Ile Val Asn Gly Thr Lys Lys Trp Ile Thr Asn Gly Met Phe Ala
275 280 285
Asp Tyr Phe Val Thr Gly Cys Arg Thr Glu Lys Gly Phe Ser Val Leu
290 295 300
Leu Ile Pro Arg Gly Glu Gly Val Glu Thr Lys Gln Ile Lys Thr Ser
305 310 315 320
Tyr Ser Thr Ala Ala Ala Thr Ala Phe Val Glu Phe Asp Asn Val Lys
325 330 335
Val Pro Val Gln Asn Leu Leu Gly Glu Glu His Lys Gly Phe Ile Val
340 345 350
Ile Met Ser Asn Phe Asn His Glu Arg Phe Met Met Val Ala Ala Val
355 360 365
Val Arg Met Ser Met Met Val Val Glu Glu Thr Met Lys Trp Ser Asn
370 375 380
Gln Arg Ile Val Phe Gly Lys Lys Leu Ile Glu Gln Pro Val Ile Arg
385 390 395 400
Gln Lys Ile Ala Arg Met Ile Ser Leu Ala Glu Ser Asn Gln Ala Trp
405 410 415
Leu Glu Ser Ile Ala Tyr Gln Met Cys Asn Met Thr Tyr Ala Gln Gln
420 425 430
Ala Lys Leu Leu Gly Gly Pro Ile Gly Leu Leu Lys Ser His Cys Thr
435 440 445
Gln Ala Ala Gly Glu Ile Ala Ser Leu Ala Thr Asn Ile Phe Gly Gly
450 455 460
Arg Gly Leu Thr Gln Ser Gly Met Gly Lys Val Ile Glu Met Phe His
465 470 475 480
Arg Thr Tyr Lys Phe Asp Ala Ile Leu Gly Gly Thr Glu Glu Ile Leu
485 490 495
Ala Asp Leu Gly Val Arg Gln Ala Met Lys Asn Phe Pro Lys Ser Met
500 505 510
Leu
<210> 55
<211> 436
<212> PRT
<213> Penicillium chrysogenum
<400> 55
Met Ala Ser Ile Ile Arg Ala Leu Arg Pro Leu Ser Arg Asn Pro Ser
1 5 10 15
Val Arg Leu Ala Gly Lys Arg Leu Ala Ala Gly Arg Pro Val Gln Ser
20 25 30
Ala Tyr Ala Phe Ser Thr Thr Pro Arg Arg Arg Glu Val Asp Leu Ser
35 40 45
Glu Leu Thr Pro Thr Pro Ile Thr Leu Leu Ser Glu Thr Glu Ser Leu
50 55 60
Met Ala Asp Ser Val Ser Lys Phe Ala Val Glu Gln Ile Gly Pro Lys
65 70 75 80
Val Arg Glu Met Asp Glu Ala Glu Thr Met Asp Ala Lys Val Val Glu
85 90 95
Gln Leu Phe Glu Gln Gly Leu Met Gly Ile Glu Val Pro Glu Glu Phe
100 105 110
Gly Gly Ala Gly Met Asn Phe Thr Ala Ala Ile Val Ala Ile Glu Glu
115 120 125
Leu Ala Arg Val Asp Pro Ser Val Ser Val Leu Val Asp Val His Asn
130 135 140
Thr Leu Val Asn Thr Ala Ile Met Lys Tyr Gly Asp Ala Lys Ala Gln
145 150 155 160
Arg Thr Trp Leu Pro Lys Leu Thr Thr Gly Thr Val Gly Ser Phe Cys
165 170 175
Leu Ser Glu Pro Ala Ser Gly Ser Asp Ala Phe Ala Leu Gln Thr Lys
180 185 190
Ala Glu Lys Thr Ala Asp Gly Tyr Lys Leu Asn Gly Ser Lys Met Trp
195 200 205
Ile Thr Asn Ser Met Glu Ala Gly Val Phe Ile Val Phe Ala Asn Ile
210 215 220
Asp Pro Ser Lys Gly Tyr Lys Gly Ile Thr Ala Phe Ile Val Glu Lys
225 230 235 240
Asp Thr Pro Gly Phe Ser Ile Ala Lys Lys Glu Lys Lys Leu Gly Ile
245 250 255
Arg Ala Ser Ser Thr Cys Val Leu Asn Phe Asp Asp Cys Val Ile Pro
260 265 270
Lys Ser Asn Leu Leu Gly Glu Glu Gly Gln Gly Tyr Lys Tyr Ala Ile
275 280 285
Ser Val Leu Asn Glu Gly Arg Ile Gly Ile Ala Ala Gln Met Thr Gly
290 295 300
Leu Ala Leu Gly Ala Trp Glu Asn Ala Ala Arg Tyr Val Trp Asn Asp
305 310 315 320
Arg Arg Gln Phe Gly Glu Leu Ile Gly Asn Phe Gln Gly Met Gln His
325 330 335
Gln Ile Ala Gln Ala Tyr Thr Glu Ile Ala Ala Ala Arg Ala Leu Val
340 345 350
Tyr Asn Ala Ala Arg Lys Lys Glu Ala Gly Gln Asp Phe Val Gln Asp
355 360 365
Ala Ala Met Ala Lys Leu Tyr Ala Ser Gln Val Ala Gly Arg Val Ser
370 375 380
Ser Ser Ala Val Glu Trp Met Gly Gly Met Gly Phe Val Arg Glu Gly
385 390 395 400
Ile Ala Glu Lys Met Phe Arg Asp Ser Lys Ile Gly Ala Ile Tyr Glu
405 410 415
Gly Thr Ser Asn Ile Gln Leu Gln Thr Ile Ala Lys Leu Leu Gln Lys
420 425 430
Gln Tyr Thr Asn
435
<210> 56
<211> 429
<212> PRT
<213> Penicillium chrysogenum
<400> 56
Met Ala Ser Leu Thr Leu Pro Ser Leu Leu Arg Thr Ser Thr Arg Ala
1 5 10 15
Val Arg Leu Asn Arg Thr Pro Ala Leu Thr Pro Cys Phe Arg Ser Ile
20 25 30
Ser Thr Lys His Pro Lys Gly Phe Ile Pro Pro Ser Glu Asp Asp Leu
35 40 45
Leu Glu Leu Arg Glu Arg Val Gln Asp Phe Thr Arg Arg Glu Ile Pro
50 55 60
Ala Asp Val Ala Ala Arg Thr Asp Glu Gln Asn Glu Phe Pro Ala Glu
65 70 75 80
Met Trp Arg Lys Met Gly Asp Ala Gly Phe Leu Gly Val Thr Ala Asn
85 90 95
Glu Glu Tyr Gly Gly Leu Gly Met Gly Tyr Gln Ala His Cys Val Val
100 105 110
Met Glu Glu Ile Ser Arg Ala Ser Gly Ser Ile Gly Leu Ser Tyr Ala
115 120 125
Ala His Ser Gln Leu Cys Val Asn Gln Leu Ser Leu Asn Gly Ser Thr
130 135 140
Glu Gln Lys Glu Arg Ile Leu Pro Gly Leu Leu Ser Gly Glu Lys Val
145 150 155 160
Gly Ala Leu Ala Met Ser Glu His Ser Ala Gly Ser Asp Val Val Ser
165 170 175
Met Lys Thr Thr Ala Lys Glu Val Asp Gly Gly Trp Leu Leu Asn Gly
180 185 190
Thr Lys Met Trp Ile Thr Asn Gly Pro Asp Ala Asp Tyr Ile Val Val
195 200 205
Tyr Ala Lys Thr Glu Pro Glu Leu Gly Ser Lys Gly Ile Thr Ala Phe
210 215 220
Leu Val Glu Lys Asp Phe Lys Gly Phe Ser Cys Ala Arg Lys Leu Asp
225 230 235 240
Lys Leu Gly Met Arg Gly Ser Asn Thr Gly Glu Leu Ile Phe Glu Asp
245 250 255
Val Phe Val Pro Arg Glu Asn Leu Leu Gly Glu Val Asn Arg Gly Val
260 265 270
Arg Val Leu Met Glu Gly Leu Asp Leu Glu Arg Leu Val Leu Ser Ala
275 280 285
Gly Pro Leu Gly Ile Met Gln Ala Ala Leu Asp Leu Val Leu Pro Tyr
290 295 300
Thr His Val Arg Lys Gln Phe Gly Ala Pro Ile Ala His Asn Gln Leu
305 310 315 320
Val Gln Gly Lys Leu Ala Asp Met Tyr Thr Lys Leu Ala Ala Ser Arg
325 330 335
Ala Tyr Thr Tyr Ala Thr Ala Arg Gln Val Asp Asn Ala Ala Val Glu
340 345 350
Pro Gly Glu Leu Thr Val Arg Thr Gln Asp Cys Ala Gly Ala Ile Leu
355 360 365
Tyr Ala Ala Glu Arg Ala Thr Glu Cys Thr Leu Asp Ala Ile Gln Leu
370 375 380
Met Gly Gly Ser Gly Tyr Ile Asn Glu Ile Pro Ala Gly Arg Leu Leu
385 390 395 400
Arg Asp Ala Lys Leu Tyr Glu Ile Gly Ala Gly Thr Ser Glu Ile Arg
405 410 415
Arg Met Val Ile Gly Arg Ala Phe Asn Lys Glu Tyr Ala
420 425
<210> 57
<211> 405
<212> PRT
<213> Penicillium Chrysogenum
<400> 57
Met Phe Ser Arg Ser Val Leu Gln Thr Ala Thr Arg Ser Val Arg Pro
1 5 10 15
Ala Ser Ala Arg Ala Val Pro Gln Leu Gly Lys Ala Pro Phe Gly Arg
20 25 30
Arg Tyr Val Ser Val Tyr Gly Tyr Thr Gln Ala Lys Ala Leu Ile Tyr
35 40 45
Ser Lys Tyr Gly Glu Pro Lys Asp Val Leu Arg Leu His Lys His Ser
50 55 60
Ile Ser Ala Pro His Ala Thr Gln Val Asn Leu Arg Leu Leu Thr Ala
65 70 75 80
Pro Met Asn Pro Ala Asp Val Asn Gln Ile Gln Gly Val Tyr Pro Ser
85 90 95
Lys Pro Pro Phe Gln Thr Glu Leu Gly Asn Val Glu Pro Ala Ala Val
100 105 110
Gly Gly Asn Glu Gly Ala Phe Glu Val Leu Ser Thr Gly Ala Gly Val
115 120 125
Lys Asn Leu Ser Lys Gly Asp Trp Val Ile Met Lys Arg Thr Gly Leu
130 135 140
Gly Thr Trp Arg Thr His Ala Gln Leu Asp Glu Ser Gln Leu Ile Lys
145 150 155 160
Val Glu Asn Lys Glu Gly Leu Thr Pro Leu Gln Val Gly Thr Val Ser
165 170 175
Val Asn Pro Val Thr Ala Tyr Arg Met Leu Arg Asp Phe Cys Glu Trp
180 185 190
Asp Trp Met Arg Ala Gly Glu Glu Trp Val Ile Gln Asn Gly Ala Asn
195 200 205
Ser Gly Val Gly Arg Ala Ala Ile Gln Leu Gly Arg Glu Trp Gly Ile
210 215 220
Lys Thr Leu Asn Val Ile Arg Gln Arg Lys Thr Pro Glu Glu Thr Glu
225 230 235 240
Ala Leu Lys Gln Glu Leu Arg Asp Leu Gly Ala Thr Val Val Ile Thr
245 250 255
Glu Glu Glu Met Leu Asn Gly Asn Phe Arg Asp Met Val His Glu Phe
260 265 270
Thr Arg Lys Gly Arg Glu Pro Ile Arg Leu Ala Leu Asn Cys Val Gly
275 280 285
Gly Lys Asn Ala Thr Ala Leu Ala Lys Thr Leu Ala Pro Asp Ser His
290 295 300
Met Val Thr Tyr Gly Ala Met Ser Lys Gln Pro Val Ala Leu Pro Ser
305 310 315 320
Gly Leu Leu Ile Phe Lys Asn Leu Ala Phe Asp Gly Phe Trp Val Ser
325 330 335
Lys Trp Gly Asp Lys Asn Pro Gln Leu Lys Glu Ser Thr Ile Lys Asp
340 345 350
Val Leu Gln Leu Thr Arg Ser Gly Lys Phe Lys Asp Ile Pro Val Asp
355 360 365
Glu Val Lys Trp Asn Trp Glu Thr Glu Gly Pro Glu Leu Ala Glu Ser
370 375 380
Val Gln Gly Thr Leu Gly Gly Tyr Arg Ser Gly Lys Gly Val Phe Thr
385 390 395 400
Phe Ser Gly Gly Asp
405
<210> 58
<211> 373
<212> PRT
<213> Bos taurus
<400> 58
Met Trp Val Cys Gly Ala Leu Cys Arg Thr Arg Ala Pro Ala Gln Leu
1 5 10 15
Gly Gln Arg Leu Leu Pro Glu Ser Arg Arg Arg Arg Pro Ala Ser Ala
20 25 30
Ser Phe Ser Ala Ser Ala Glu Pro Ser Arg Val Arg Ala Leu Val Tyr
35 40 45
Gly His His Gly Asp Pro Ala Lys Val Val Glu Leu Lys Asn Leu Glu
50 55 60
Leu Ala Ala Val Gly Gly Ser His Val His Val Lys Met Leu Ala Ala
65 70 75 80
Pro Ile Asn Pro Ser Asp Ile Asn Met Ile Gln Gly Asn Tyr Gly Leu
85 90 95
Leu Pro Gln Leu Pro Ala Val Gly Gly Asn Glu Gly Val Gly Gln Val
100 105 110
Val Ala Val Gly Ser Gly Val Thr Gly Val Lys Pro Gly Asp Trp Val
115 120 125
Ile Pro Ala Asn Pro Gly Leu Gly Thr Trp Arg Thr Glu Ala Val Phe
130 135 140
Gly Glu Glu Glu Leu Ile Thr Val Pro Ser Asp Ile Pro Leu Gln Ser
145 150 155 160
Ala Ala Thr Leu Gly Val Asn Pro Cys Thr Ala Tyr Arg Met Leu Val
165 170 175
Asp Phe Glu Arg Leu Arg Pro Arg Asp Ser Ile Ile Gln Asn Ala Ser
180 185 190
Asn Ser Gly Val Gly Gln Ala Val Ile Gln Ile Ala Ala Ala Arg Gly
195 200 205
Leu Arg Thr Ile Asn Val Leu Arg Asp Thr Pro Asp Leu Gln Lys Leu
210 215 220
Thr Asp Thr Leu Lys Asn Leu Gly Ala Asn His Val Val Thr Glu Glu
225 230 235 240
Glu Leu Arg Lys Pro Glu Met Lys Ser Phe Phe Lys Asp Val Pro Gln
245 250 255
Pro Arg Leu Ala Leu Asn Cys Val Gly Gly Lys Ser Ser Thr Glu Leu
260 265 270
Leu Arg His Leu Ala Pro Gly Gly Thr Met Val Thr Tyr Gly Gly Met
275 280 285
Ala Lys Gln Pro Val Ile Ala Ser Val Ser Gln Leu Ile Phe Lys Asp
290 295 300
Leu Lys Leu Arg Gly Phe Trp Leu Ser Gln Trp Lys Lys Asp His Ser
305 310 315 320
Pro Asp Gln Phe Lys Glu Leu Ile Leu Thr Leu Cys Asp Leu Ile Arg
325 330 335
Arg Gly Gln Leu Thr Ala Pro Ala Cys Ser Glu Val Pro Leu Gln Asp
340 345 350
Tyr Leu Cys Ala Leu Glu Ala Ser Thr Gln Pro Phe Val Ser Ser Lys
355 360 365
Gln Ile Leu Thr Met
370
<210> 59
<211> 302
<212> PRT
<213> Cavia sp.
<400> 59
Met Gly Ser Trp Thr Lys Cys Gln Ser Cys Leu Ala Pro Gly Leu Leu
1 5 10 15
Gln Asn Arg Ala Ala Ile Val Thr Gly Gly Gly Thr Gly Ile Gly Lys
20 25 30
Ala Ile Ala Lys Glu Leu Leu His Leu Gly Cys Asn Val Val Ile Ala
35 40 45
Ser Arg Lys Phe Asp Arg Leu Arg Ala Ala Ala Glu Glu Leu Lys Ala
50 55 60
Thr Leu Pro Pro Ser Asn Lys Ala Glu Val Thr Pro Ile Gln Cys Asn
65 70 75 80
Ile Arg Lys Glu Glu Glu Val Asn Asn Leu Met Lys Ser Thr Leu Ala
85 90 95
Leu Tyr Gly Lys Ile Asp Phe Leu Val Asn Asn Gly Gly Gly Gln Phe
100 105 110
Trp Ser Ser Pro Glu His Ile Ser Ser Lys Gly Trp His Ala Val Ile
115 120 125
Glu Thr Asn Leu Thr Gly Thr Phe Tyr Met Cys Lys Ala Ala Tyr Asn
130 135 140
Ser Trp Met Lys Glu His Gly Gly Ala Ile Val Asn Ile Ile Ile Leu
145 150 155 160
Leu Asn Gly Gln Pro Phe Val Ala His Ser Gly Ala Ala Arg Gly Gly
165 170 175
Val Tyr Asn Leu Thr Lys Ser Leu Ala Leu Gly Trp Ala Arg Ser Gly
180 185 190
Ile Arg Ile Asn Cys Val Ala Pro Gly Thr Val Tyr Ser Gln Thr Ala
195 200 205
Met Asp Asn Tyr Gly Asp Met Gly Lys Thr Leu Phe Ala Asp Ala Phe
210 215 220
Gln Lys Ile Pro Ala Lys Arg Leu Gly Val Pro Glu Glu Val Ser Ser
225 230 235 240
Leu Val Cys Phe Leu Leu Ser Pro Ala Ala Ser Phe Ile Thr Gly Gln
245 250 255
Leu Val Asn Val Asp Gly Gly Gln Ser Leu Tyr Cys Gln Asn His Asp
260 265 270
Ile Pro Asp His Asp Asn Trp Pro Glu Gly Val Gly Asp Leu Ser Thr
275 280 285
Val Lys Lys Met Lys Glu Ser Phe Lys Gln Lys Ala Lys Leu
290 295 300
<210> 60
<211> 386
<212> PRT
<213> Candida tropicalis
<400> 60
Met Tyr Ser Val Leu Lys Gln Ser Ile Arg Pro Arg Leu Leu Ala Thr
1 5 10 15
His Asn Gln Phe Arg Thr Met Ile Thr Ala Gln Ala Val Leu Tyr Thr
20 25 30
Gln His Gly Glu Pro Lys Asp Val Leu Phe Thr Gln Ser Phe Glu Ile
35 40 45
Asp Asp Asp Asn Leu Ala Pro Asn Glu Val Ile Val Lys Thr Leu Gly
50 55 60
Ser Pro Val Asn Pro Ser Asp Ile Asn Gln Ile Gln Gly Val Tyr Pro
65 70 75 80
Ser Lys Pro Ala Lys Thr Thr Gly Phe Gly Thr Thr Glu Pro Ala Ala
85 90 95
Pro Cys Gly Asn Glu Gly Leu Phe Glu Val Ile Lys Val Gly Ser Asn
100 105 110
Val Ser Ser Leu Glu Ala Gly Asp Trp Val Ile Pro Ser His Val Asn
115 120 125
Phe Gly Thr Trp Arg Thr His Ala Leu Gly Asn Asp Asp Asp Phe Ile
130 135 140
Lys Leu Pro Asn Pro Ala Gln Ser Lys Ala Asn Gly Lys Pro Asn Gly
145 150 155 160
Leu Thr Ile Asn Gln Gly Ala Thr Ile Ser Val Asn Pro Leu Thr Ala
165 170 175
Tyr Leu Met Leu Thr His Tyr Val Lys Leu Thr Pro Gly Lys Asp Trp
180 185 190
Phe Ile Gln Asn Gly Gly Thr Ser Ala Val Gly Lys Tyr Ala Ser Gln
195 200 205
Ile Gly Lys Leu Leu Asn Phe Asn Ser Ile Ser Val Ile Arg Asp Arg
210 215 220
Pro Asn Leu Asp Glu Val Val Ala Ser Leu Lys Glu Leu Gly Ala Thr
225 230 235 240
Gln Val Ile Thr Glu Asp Gln Asn Asn Ser Arg Glu Phe Gly Pro Thr
245 250 255
Ile Lys Glu Trp Ile Lys Gln Ser Gly Gly Glu Ala Lys Leu Ala Leu
260 265 270
Asn Cys Val Gly Gly Lys Ser Ser Thr Gly Ile Ala Arg Lys Leu Asn
275 280 285
Asn Asn Gly Leu Met Leu Thr Tyr Gly Gly Met Ser Phe Gln Pro Val
290 295 300
Thr Ile Pro Thr Ser Leu Tyr Ile Phe Lys Asn Phe Thr Ser Ala Gly
305 310 315 320
Phe Trp Val Thr Glu Leu Leu Lys Asn Asn Lys Glu Leu Lys Thr Ser
325 330 335
Thr Leu Asn Gln Ile Ile Ala Trp Tyr Glu Glu Gly Lys Leu Thr Asp
340 345 350
Ala Lys Ser Ile Glu Thr Leu Tyr Asp Gly Thr Lys Pro Leu His Glu
355 360 365
Leu Tyr Gln Asp Gly Val Ala Asn Ser Lys Asp Gly Lys Gln Leu Ile
370 375 380
Thr Tyr
385
<210> 61
<211> 379
<212> PRT
<213> Clostridium kluyveri
<400> 61
Met Asp Phe Thr Leu Thr Asn Glu Gln Lys Phe Val Glu Gln Met Val
1 5 10 15
Ser Glu Phe Thr Glu Asn Glu Val Lys Pro Ile Ala Ala Glu Ile Asp
20 25 30
Glu Thr Glu Arg Phe Pro Leu Glu Thr Val Glu Lys Phe Ala Lys Tyr
35 40 45
Gly Met Met Gly Met Pro Phe Pro Val Glu Tyr Gly Gly Ser Gly Thr
50 55 60
Asp Tyr Leu Ser Tyr Ile Ile Ala Val Glu Gly Leu Ala Lys Ser Cys
65 70 75 80
Thr Ser Ser Ser Thr Ile Leu Ser Ala His Thr Ser Leu Cys Ala Ala
85 90 95
Pro Ile Tyr Asp Trp Gly Thr Glu Glu Gln Lys Gln Lys Tyr Leu Val
100 105 110
Pro Leu Ala Lys Gly Glu Lys Leu Gly Ala Phe Gly Leu Thr Glu Pro
115 120 125
Asn Ala Gly Thr Asp Ala Ala Gly Gln Gln Thr Thr Ala Val Leu Glu
130 135 140
Gly Asp His Tyr Val Leu Asn Gly Gln Lys Ile Phe Ile Thr Asn Gly
145 150 155 160
Ala Tyr Ala Asp Thr Phe Val Ile Phe Ala Met Thr Asp Arg Ser Lys
165 170 175
Gly Thr Arg Gly Ile Thr Ala Phe Ile Val Glu Lys Asp Phe Pro Gly
180 185 190
Phe Ser Ile Gly Lys Ser Glu Asp Lys Leu Gly Ile Arg Ala Ser Ser
195 200 205
Thr Thr Glu Leu Ile Phe Glu Asn Cys Ile Val Pro Lys Glu Asn Met
210 215 220
Leu Gly Lys Glu Gly Lys Gly Phe Thr Val Ala Met His Thr Leu Asp
225 230 235 240
Gly Gly Arg Ile Gly Ile Ala Ala Gln Ala Leu Gly Leu Ala Glu Gly
245 250 255
Ala Leu Ala Glu Ala Leu Asn Tyr Met Lys Glu Arg Lys Gln Phe Gly
260 265 270
Lys Ala Leu Tyr Lys Phe Gln Gly Leu Ala Trp Met Val Ala Glu Leu
275 280 285
Asp Thr Lys Ile Glu Ala Val Lys Gln Leu Val Tyr Lys Ala Ala Val
290 295 300
Asn Lys Gln Met Gly Leu Pro Tyr Ser Val Glu Ala Ala Arg Ala Lys
305 310 315 320
Leu Ala Ala Ala Thr Val Ala Met Glu Thr Thr Thr Lys Val Val Gln
325 330 335
Ile Phe Gly Gly Tyr Gly Phe Thr Lys Asp Tyr Pro Val Glu Arg Met
340 345 350
Met Arg Asp Ala Lys Ile Thr Glu Ile Tyr Glu Gly Thr Ser Gln Val
355 360 365
Gln Lys Met Val Ile Ser Ala Asn Leu Phe Lys
370 375
<210> 62
<211> 562
<212> PRT
<213> Clostridium kluyveri
<400> 62
Met Asn Phe Glu Leu Thr Lys Glu Gln Gln Met Ile Arg Asp Asn Val
1 5 10 15
Arg Lys Phe Ala Glu Ala Lys Ile Glu Pro Ile Ala Phe Gln Leu Asp
20 25 30
Glu Lys Asn Ile Phe Pro Glu Glu Ile Val Asn Glu Met Gly Asp Leu
35 40 45
Ser Ile Met Gly Leu Pro Tyr Pro Lys Glu Tyr Gly Gly Ala Gly Lys
50 55 60
Asp Val Leu Ser Tyr Ala Ile Ala Val Glu Glu Leu Ser Arg Val Asp
65 70 75 80
Ala Gly Val Gly Val Ile Leu Ser Ala His Thr Ser Leu Gly Thr Trp
85 90 95
Pro Ile Met Glu Phe Gly Thr Lys Glu Gln Lys Glu Lys Tyr Leu Val
100 105 110
Pro Leu Ala Ser Gly Lys Lys Ile Ala Ala Phe Gly Leu Thr Glu Pro
115 120 125
Asn Ala Gly Ser Asp Ala Gly Lys Thr Glu Thr Thr Ala Val Leu Glu
130 135 140
Gly Asp His Tyr Val Ile Asn Gly Ser Lys Val Phe Ile Thr Asn Ala
145 150 155 160
Asp Tyr Ala Asp Thr Tyr Val Ile Phe Ala Val Thr Thr Pro Gly Leu
165 170 175
Gly Thr Lys Gly Ile Ser Ala Phe Ile Ile Glu Lys Gly Met Asp Gly
180 185 190
Phe Thr Phe Gly Thr His Tyr Asn Lys Met Gly Ile Arg Ser Ser Ala
195 200 205
Thr Ala Glu Leu Leu Phe Lys Asn Leu Lys Val Pro Lys Tyr Asn Leu
210 215 220
Leu Gly Lys Glu Asn Glu Gly Phe Lys Ile Ala Met Gln Thr Leu Glu
225 230 235 240
Gly Gly Arg Ile Gly Ile Ala Ala Gln Ala Leu Gly Ile Ala Gln Gly
245 250 255
Ala Tyr Glu Lys Ala Leu Ser Tyr Ser Lys Glu Arg Val Gln Phe Gly
260 265 270
Lys Pro Ile Ser Arg Gln Gln Ser Ile Ala Phe Lys Leu Ala Asp Met
275 280 285
Ala Thr Lys Ile Arg Ala Ala Arg Phe Met Val Tyr Ser Ala Ala Val
290 295 300
Leu Lys Gln Glu His Lys Asn Tyr Gly Met Glu Ser Ala Met Ala Lys
305 310 315 320
Leu Tyr Ala Ser Asp Ile Cys Leu Glu Val Val Asn Asp Ala Val Gln
325 330 335
Ile Tyr Gly Gly Ser Gly Phe Ile Lys Gly Phe Pro Val Glu Arg Met
340 345 350
Tyr Arg Asp Ala Lys Ile Cys Thr Ile Tyr Glu Gly Thr Asn Glu Ile
355 360 365
Gln Arg Leu Ile Ile Ser Asn Asp Ile Leu Gly Lys Pro Lys Lys Glu
370 375 380
Pro Ile Glu Glu Asn Lys Glu Asn Lys Val Asn Lys Ala Lys Pro Ile
385 390 395 400
Thr Gly Asn Arg Arg Arg Val Ile Ile Lys Glu Gly Ser Pro Lys Glu
405 410 415
Lys Val Asp Ala Phe Leu Asn Tyr Ile Lys Ser Glu Asn Ile Asp Ile
420 425 430
Asn Lys Ser Glu Ala Ser Lys Gly Ser Ile Ala Asp Ala Asp Lys Val
435 440 445
Cys Ser Ile Gly Leu Gly Leu Lys Asp Lys Lys Asp Leu Pro Leu Ile
450 455 460
Gln Ser Leu Ala Asp Thr Val Gly Ala Glu Leu Gly Cys Ser Arg Pro
465 470 475 480
Val Ala Glu Glu Arg Glu Trp Leu Pro Leu Asp Arg Tyr Val Gly Ile
485 490 495
Ser Gly Gln Lys Phe Gly Gly Thr Phe Tyr Leu Ala Ile Gly Ile Ser
500 505 510
Gly Gln Val Gln His Leu Lys Gly Ile Glu Asn Ala Gly Ile Ile Thr
515 520 525
Ala Ile Asn Ile Asp Glu Asp Ala Pro Ile Phe Lys Ser Ser Asp Tyr
530 535 540
Gly Ile Val Gly Asp Leu Tyr Glu Ile Val Pro Leu Leu Ile Glu Ala
545 550 555 560
Leu Lys
<210> 63
<211> 539
<212> PRT
<213> Euglena gracilis
<400> 63
Met Ser Cys Pro Ala Ser Pro Ser Ala Ala Val Val Ser Ala Gly Ala
1 5 10 15
Leu Cys Leu Cys Val Ala Thr Val Leu Leu Ala Thr Gly Ser Asn Pro
20 25 30
Thr Ala Leu Ser Thr Ala Ser Thr Arg Ser Pro Thr Ser Leu Val Arg
35 40 45
Gly Val Asp Arg Gly Leu Met Arg Pro Thr Thr Ala Ala Ala Leu Thr
50 55 60
Thr Met Arg Glu Val Pro Gln Met Ala Glu Gly Phe Ser Gly Glu Ala
65 70 75 80
Thr Ser Ala Trp Ala Ala Ala Gly Pro Gln Trp Ala Ala Pro Leu Val
85 90 95
Ala Ala Ala Ser Ser Ala Leu Ala Leu Trp Trp Trp Ala Ala Arg Arg
100 105 110
Ser Val Arg Arg Pro Leu Ala Ala Leu Ala Glu Leu Pro Thr Ala Val
115 120 125
Thr His Leu Ala Pro Pro Met Ala Met Phe Thr Thr Thr Ala Lys Val
130 135 140
Ile Gln Pro Lys Ile Arg Gly Phe Ile Cys Thr Thr Thr His Pro Ile
145 150 155 160
Gly Cys Glu Lys Arg Val Gln Glu Glu Ile Ala Tyr Ala Arg Ala His
165 170 175
Pro Pro Thr Ser Pro Gly Pro Lys Arg Val Leu Val Ile Gly Cys Ser
180 185 190
Thr Gly Tyr Gly Leu Ser Thr Arg Ile Thr Ala Ala Phe Gly Tyr Gln
195 200 205
Ala Ala Thr Leu Gly Val Phe Leu Ala Gly Pro Pro Thr Lys Gly Arg
210 215 220
Pro Ala Ala Ala Gly Trp Tyr Asn Thr Val Ala Phe Glu Lys Ala Ala
225 230 235 240
Leu Glu Ala Gly Leu Tyr Ala Arg Ser Leu Asn Gly Asp Ala Phe Asp
245 250 255
Ser Thr Thr Lys Ala Arg Thr Val Glu Ala Ile Lys Arg Asp Leu Gly
260 265 270
Thr Val Asp Leu Val Val Tyr Ser Ile Ala Ala Pro Lys Arg Thr Asp
275 280 285
Pro Ala Thr Gly Val Leu His Lys Ala Cys Leu Lys Pro Ile Gly Ala
290 295 300
Thr Tyr Thr Asn Arg Thr Val Asn Thr Asp Lys Ala Glu Val Thr Asp
305 310 315 320
Val Ser Ile Glu Pro Ala Ser Pro Glu Glu Ile Ala Asp Thr Val Lys
325 330 335
Val Met Gly Gly Glu Asp Trp Glu Leu Trp Ile Gln Ala Leu Ser Glu
340 345 350
Ala Gly Val Leu Ala Glu Gly Ala Lys Thr Val Ala Tyr Ser Tyr Ile
355 360 365
Gly Pro Glu Met Thr Trp Pro Val Tyr Trp Ser Gly Thr Ile Gly Glu
370 375 380
Ala Lys Lys Asp Val Glu Lys Ala Ala Lys Arg Ile Thr Gln Gln Tyr
385 390 395 400
Gly Cys Pro Ala Tyr Pro Val Val Ala Lys Ala Leu Val Thr Gln Ala
405 410 415
Ser Ser Ala Ile Pro Val Val Pro Leu Tyr Ile Cys Leu Leu Tyr Arg
420 425 430
Val Met Lys Glu Lys Gly Thr His Glu Gly Cys Ile Glu Gln Met Val
435 440 445
Arg Leu Leu Thr Thr Lys Leu Tyr Pro Glu Asn Gly Ala Pro Ile Val
450 455 460
Asp Glu Ala Gly Arg Val Arg Val Asp Asp Trp Glu Met Ala Glu Asp
465 470 475 480
Val Gln Gln Ala Val Lys Asp Leu Trp Ser Gln Val Ser Thr Ala Asn
485 490 495
Leu Lys Asp Ile Ser Asp Phe Ala Gly Tyr Gln Thr Glu Phe Leu Arg
500 505 510
Leu Phe Gly Phe Gly Ile Asp Gly Val Asp Tyr Asp Gln Pro Val Asp
515 520 525
Val Glu Ala Asp Leu Pro Ser Ala Ala Gln Gln
530 535
<210> 64
<211> 303
<212> PRT
<213> Homo sapiens
<400> 64
Met Ala Ser Trp Ala Lys Gly Arg Ser Tyr Leu Ala Pro Gly Leu Leu
1 5 10 15
Gln Gly Gln Val Ala Ile Val Thr Gly Gly Ala Thr Gly Ile Gly Lys
20 25 30
Ala Ile Val Lys Glu Leu Leu Glu Leu Gly Ser Asn Val Val Ile Ala
35 40 45
Ser Arg Lys Leu Glu Arg Leu Lys Ser Ala Ala Asp Glu Leu Gln Ala
50 55 60
Asn Leu Pro Pro Thr Lys Gln Ala Arg Val Ile Pro Ile Gln Cys Asn
65 70 75 80
Ile Arg Asn Glu Glu Glu Val Asn Asn Leu Val Lys Ser Thr Leu Asp
85 90 95
Thr Phe Gly Lys Ile Asn Phe Leu Val Asn Asn Gly Gly Gly Gln Phe
100 105 110
Leu Ser Pro Ala Glu His Ile Ser Ser Lys Gly Trp His Ala Val Leu
115 120 125
Glu Thr Asn Leu Thr Gly Thr Phe Tyr Met Cys Lys Ala Val Tyr Ser
130 135 140
Ser Trp Met Lys Glu His Gly Gly Ser Ile Val Asn Ile Ile Val Pro
145 150 155 160
Thr Lys Ala Gly Phe Pro Leu Ala Val His Ser Gly Ala Ala Arg Ala
165 170 175
Gly Val Tyr Asn Leu Thr Lys Ser Leu Ala Leu Glu Trp Ala Cys Ser
180 185 190
Gly Ile Arg Ile Asn Cys Val Ala Pro Gly Val Ile Tyr Ser Gln Thr
195 200 205
Ala Val Glu Asn Tyr Gly Ser Trp Gly Gln Ser Phe Phe Glu Gly Ser
210 215 220
Phe Gln Lys Ile Pro Ala Lys Arg Ile Gly Val Pro Glu Glu Val Ser
225 230 235 240
Ser Val Val Cys Phe Leu Leu Ser Pro Ala Ala Ser Phe Ile Thr Gly
245 250 255
Gln Ser Val Asp Val Asp Gly Gly Arg Ser Leu Tyr Thr His Ser Tyr
260 265 270
Glu Val Pro Asp His Asp Asn Trp Pro Lys Gly Ala Gly Asp Leu Ser
275 280 285
Val Val Lys Lys Met Lys Glu Thr Phe Lys Glu Lys Ala Lys Leu
290 295 300
<210> 65
<211> 303
<212> PRT
<213> Mus musculus
<400> 65
Met Gly Ser Trp Lys Thr Gly Gln Ser Tyr Leu Ala Ala Gly Leu Leu
1 5 10 15
Lys Asn Gln Val Ala Val Val Thr Gly Gly Gly Thr Gly Ile Gly Lys
20 25 30
Ala Val Ser Arg Glu Leu Leu His Leu Gly Cys Asn Val Val Ile Ala
35 40 45
Ser Arg Lys Leu Asp Arg Leu Thr Ala Ala Val Asp Glu Leu Arg Ala
50 55 60
Ser Leu Pro Pro Ser Ser Ser Ala Glu Val Ser Ala Ile Gln Cys Asn
65 70 75 80
Ile Arg Lys Glu Glu Glu Val Ser Asn Leu Val Lys Ser Thr Leu Ala
85 90 95
Lys Tyr Gly Lys Ile Asn Phe Leu Val Asn Asn Gly Gly Gly Gln Phe
100 105 110
Met Ala Pro Val Glu Asp Ile Thr Ala Lys Gly Trp His Ala Val Ile
115 120 125
Glu Thr Asn Leu Thr Gly Thr Phe Tyr Met Cys Lys Glu Val Tyr Asn
130 135 140
Ser Trp Met Arg Glu His Gly Gly Ser Ile Val Asn Ile Ile Val Leu
145 150 155 160
Leu Asn Asn Gly Phe Pro Thr Ala Ala His Thr Gly Ala Ala Arg Glu
165 170 175
Gly Val Tyr Asn Leu Thr Lys Ser Met Ala Leu Ala Trp Ala Ser Ser
180 185 190
Gly Val Arg Ile Asn Cys Val Ala Pro Gly Thr Ile Tyr Ser Gln Thr
195 200 205
Ala Val Asp Asn Tyr Gly Glu Met Gly Gln Thr Leu Phe Glu Met Ala
210 215 220
Phe Asp Ser Ile Pro Val Ser Ala Leu Gly Val Pro Glu Glu Ile Ser
225 230 235 240
Leu Leu Ala Arg Phe Leu Leu Ser Pro Ala Ala Ser Tyr Ile Thr Gly
245 250 255
Gln Leu Ile Asn Val Asp Gly Gly Gln Ala Leu His Thr His Ala Phe
260 265 270
Ser Ile Pro Asp His Asp Asn Trp Pro Val Gly Ala Gly Asp Leu Ser
275 280 285
Ile Val Lys Arg Ile Lys Glu Ser Phe Lys Lys Lys Ala Lys Leu
290 295 300
<210> 66
<211> 373
<212> PRT
<213> Rattus norvegicus
<400> 66
Met Leu Val Ser Arg Arg Leu Thr Gly Ala Arg Ala Arg Ala Pro Leu
1 5 10 15
Leu Ala Ser Leu Leu Glu Ala Trp Cys Arg Gln Gly Arg Thr Thr Ser
20 25 30
Ser Tyr Ser Ala Phe Ser Glu Pro Ser His Val Arg Ala Leu Val Tyr
35 40 45
Gly Asn His Gly Asp Pro Ala Lys Val Ile Gln Leu Lys Asn Leu Glu
50 55 60
Leu Thr Ala Val Glu Gly Ser Asp Val His Val Lys Met Leu Ala Ala
65 70 75 80
Pro Ile Asn Pro Ser Asp Ile Asn Met Ile Gln Gly Asn Tyr Gly Leu
85 90 95
Leu Pro Lys Leu Pro Ala Val Gly Gly Asn Glu Gly Val Gly Gln Val
100 105 110
Ile Ala Val Gly Ser Ser Val Ser Gly Leu Lys Pro Gly Asp Trp Val
115 120 125
Ile Pro Ala Asn Ala Gly Leu Gly Thr Trp Arg Thr Glu Ala Val Phe
130 135 140
Ser Glu Glu Ala Leu Ile Gly Val Pro Lys Asp Ile Pro Leu Gln Ser
145 150 155 160
Ala Ala Thr Leu Gly Val Asn Pro Cys Thr Ala Tyr Arg Met Leu Val
165 170 175
Asp Phe Glu Gln Leu Gln Pro Gly Asp Ser Val Ile Gln Asn Ala Ser
180 185 190
Asn Ser Gly Val Gly Gln Ala Val Ile Gln Ile Ala Ser Ala Leu Gly
195 200 205
Leu Lys Thr Ile Asn Val Ile Arg Asp Arg Pro Asp Ile Lys Lys Leu
210 215 220
Thr Asp Arg Leu Lys Asp Leu Gly Ala Asp Tyr Val Leu Thr Glu Glu
225 230 235 240
Glu Leu Arg Met Pro Glu Thr Lys Asn Ile Phe Lys Asp Leu Pro Leu
245 250 255
Pro Arg Leu Ala Leu Asn Cys Val Gly Gly Lys Ser Ser Thr Glu Leu
260 265 270
Leu Arg His Leu Ala Pro Gly Gly Thr Met Val Thr Tyr Gly Gly Met
275 280 285
Ala Lys Gln Pro Val Thr Ala Ser Val Ser Met Leu Ile Phe Lys Asp
290 295 300
Leu Lys Leu Arg Gly Phe Trp Leu Ser Gln Trp Lys Lys Asn His Ser
305 310 315 320
Pro Asp Glu Phe Lys Glu Leu Ile Leu Ile Leu Cys Asn Leu Ile Arg
325 330 335
Gln Gly Gln Leu Thr Ala Pro Ala Trp Ser Gly Ile Pro Leu Gln Asp
340 345 350
Tyr Gln Gln Ala Leu Glu Ala Ser Met Lys Pro Phe Val Ser Leu Lys
355 360 365
Gln Ile Leu Thr Met
370
<210> 67
<211> 380
<212> PRT
<213> Saccharomyces cerevisiae
<400> 67
Met Leu Pro Thr Phe Lys Arg Tyr Met Ser Ser Ser Ala His Gln Ile
1 5 10 15
Pro Lys His Phe Lys Ser Leu Ile Tyr Ser Thr His Glu Val Glu Asp
20 25 30
Cys Thr Lys Val Leu Ser Val Lys Asn Tyr Thr Pro Lys Gln Asp Leu
35 40 45
Ser Gln Ser Ile Val Leu Lys Thr Leu Ala Phe Pro Ile Asn Pro Ser
50 55 60
Asp Ile Asn Gln Leu Gln Gly Val Tyr Pro Ser Arg Pro Glu Lys Thr
65 70 75 80
Tyr Asp Tyr Ser Thr Asp Glu Pro Ala Ala Ile Ala Gly Asn Glu Gly
85 90 95
Val Phe Glu Val Val Ser Leu Pro Ser Gly Ser Ser Lys Gly Asp Leu
100 105 110
Lys Leu Gly Asp Arg Val Ile Pro Leu Gln Ala Asn Gln Gly Thr Trp
115 120 125
Ser Asn Tyr Arg Val Phe Ser Ser Ser Ser Asp Leu Ile Lys Val Asn
130 135 140
Asp Leu Asp Leu Phe Ser Ala Ala Thr Val Ser Val Asn Gly Cys Thr
145 150 155 160
Gly Phe Gln Leu Val Ser Asp Tyr Ile Asp Trp Asn Ser Asn Gly Asn
165 170 175
Glu Trp Ile Ile Gln Asn Ala Gly Thr Ser Ser Val Ser Lys Ile Val
180 185 190
Thr Gln Val Ala Lys Ala Lys Gly Ile Lys Thr Leu Ser Val Ile Arg
195 200 205
Asp Arg Asp Asn Phe Asp Glu Val Ala Lys Val Leu Glu Asp Lys Tyr
210 215 220
Gly Ala Thr Lys Val Ile Ser Glu Ser Gln Asn Asn Asp Lys Thr Phe
225 230 235 240
Ala Lys Glu Val Leu Ser Lys Ile Leu Gly Glu Asn Ala Arg Val Arg
245 250 255
Leu Ala Leu Asn Ser Val Gly Gly Lys Ser Ser Ala Ser Ile Ala Arg
260 265 270
Lys Leu Glu Asn Asn Ala Leu Met Leu Thr Tyr Gly Gly Met Ser Lys
275 280 285
Gln Pro Val Thr Leu Pro Thr Ser Leu His Ile Phe Lys Gly Leu Thr
290 295 300
Ser Lys Gly Tyr Trp Val Thr Glu Lys Asn Lys Lys Asn Pro Gln Ser
305 310 315 320
Lys Ile Asp Thr Ile Ser Asp Phe Ile Lys Met Tyr Asn Tyr Gly His
325 330 335
Ile Ile Ser Pro Arg Asp Glu Ile Glu Thr Leu Thr Trp Asn Thr Asn
340 345 350
Thr Thr Thr Asp Glu Gln Leu Leu Glu Leu Val Lys Lys Gly Ile Thr
355 360 365
Gly Lys Gly Lys Lys Lys Met Val Val Leu Glu Trp
370 375 380
<210> 68
<211> 365
<212> PRT
<213> Penicillium Chrysogenum
<400> 68
Met Ser Lys Arg Val Thr Ile Phe Asp Pro Pro Glu Gln Asp His Ser
1 5 10 15
Lys Ala Leu Ile Glu Asn Val Leu Asp Leu Thr Pro Val Val Asp Leu
20 25 30
Gly Pro Asp Val Phe Thr Asn Thr Arg Pro Leu Trp His Pro Pro Gly
35 40 45
Ala Arg Gly Ile Tyr Gly Gly Ala Ala Ile Ala Gln Ser Leu Ser Ala
50 55 60
Ala Met Arg Thr Val Pro Ala Asp Tyr Ala Val His Ser Met His Cys
65 70 75 80
Tyr Phe Val Leu Ala Gly Asp Ser Glu Ile Pro Ile Leu Tyr His Val
85 90 95
Glu Arg Val Arg Asp Gly Arg Ser Phe Val Thr Arg Thr Val Gln Ala
100 105 110
Arg Gln Arg Gly Arg Pro Ile Phe Thr Thr Thr Leu Ser Phe Ser Arg
115 120 125
Val Gly Ser Gly Gly Glu Lys Thr Leu His His Ala Val Ser Lys Pro
130 135 140
Asp Val Pro Leu Pro Glu Glu Ala Glu Pro Gly Ser Leu Lys Ala Leu
145 150 155 160
Ser Asn Ala Gly Gly Gly Pro Phe Glu Ser Arg Lys Ala Gly Ile Leu
165 170 175
Asn Arg Thr Ser Pro Asn Pro Glu Asp Lys Lys Val Arg Arg Tyr Ile
180 185 190
Arg Ala Arg Gly Ala Ile Ser Glu Glu Gly Gly Tyr Gln Ala His Leu
195 200 205
Ser Ala Leu Ala Tyr Ile Thr Asp Ser Tyr Phe Ile Gly Thr Val Ser
210 215 220
Ile Val His Asp Val Pro Arg Phe Ser Ser Pro Ala Glu Leu Glu Lys
225 230 235 240
Leu Leu Asn Ala Leu Lys Asn Pro Ser Asp Leu Asp Asp Glu Asp Ile
245 250 255
Thr Arg Ala Leu Arg Glu Leu Lys Glu Glu Glu Ala Ala Glu Leu Arg
260 265 270
Arg Arg Leu Glu Gly Ala Leu Asn Arg Ala Thr Gly Pro Lys Glu Val
275 280 285
Asp His Lys Glu Val Gly Met Met Val Ser Leu Asp His Ser Ile Tyr
290 295 300
Phe His Asn Pro Arg Ala Phe Arg Ala Asp Glu Trp Met Leu Ser Glu
305 310 315 320
Ile Glu Ser Pro Trp Ala Gly Glu Gly Arg Gly Leu Ala Ile Gln Lys
325 330 335
Leu Trp Ser Lys Asp Gly Val Leu Ile Ala Thr Cys Thr Gln Glu Val
340 345 350
Cys Ile Cys Phe Tyr Ser Leu Tyr Ser Gly Gly Gln Cys
355 360 365
<210> 69
<211> 525
<212> PRT
<213> Penicillium Chrysogenum
<400> 69
Met Ser Ala Ser Ala Leu Leu Lys Ser Arg Val Arg Arg Pro Ser Tyr
1 5 10 15
Leu Lys Lys Leu Ala Lys Pro Glu Asp Leu Leu His His Phe Pro Asn
20 25 30
Gly Thr Tyr Ile Gly Trp Ser Gly Phe Thr Gly Val Gly Tyr Pro Lys
35 40 45
Met Thr Pro Ile Ala Leu Ala Asp His Val Glu Lys Asn Asn Leu Gln
50 55 60
Gly Gln Leu Lys Tyr Asn Leu Phe Val Gly Ala Ser Ser Gly Ala Glu
65 70 75 80
Thr Glu Asn Arg Trp Ala Arg Leu Asn Met Ile Glu Arg Arg Ser Pro
85 90 95
His Gln Val Gly Lys Glu Ile Ala Lys Gly Ile Asn Asn Gly Gln Ile
100 105 110
Lys Phe Phe Asp Lys His Leu Ser Met Phe Pro Ser Asp Leu Val Tyr
115 120 125
Gly Tyr Tyr Thr Leu Asn Lys Ser Lys Pro Thr Ile Asp Val Ala Val
130 135 140
Ile Glu Ala Ser Ala Ile Thr Glu Asn Gly Gly Ile Ile Pro Gly Ala
145 150 155 160
Ser Val Gly Ala Ser Pro Glu Leu Ile Gln Met Ala Asp Lys Val Ile
165 170 175
Ile Glu Val Asn Thr Ser Met Pro Ser Phe Glu Gly Leu His Asp Ile
180 185 190
Thr Cys Ser Asp Leu Pro Pro Gly Arg Lys Pro Tyr Leu Ile Met Ala
195 200 205
Pro Glu Asp Arg Ile Gly Thr Ser Tyr Ile Pro Ile Asp Pro Glu Lys
210 215 220
Val Val Ala Ile Val Glu Ser Asn Tyr Pro Asp Gln Thr Leu Pro Asn
225 230 235 240
Ala Pro Glu Asp Glu Gly Ser Gln Ala Ile Ala Ser Asn Leu Ile Glu
245 250 255
Phe Leu Lys His Glu Val Asn His Gly Arg Leu Pro Pro Asn Leu Leu
260 265 270
Pro Ile Gln Ser Gly Ile Gly Asn Ile Ala Asn Ala Val Val Gly Gly
275 280 285
Leu Ser Lys Gly Gly Ala Asp Phe Lys Asn Leu Lys Val Trp Thr Glu
290 295 300
Val Leu Gln Asp Ser Phe Leu Asp Leu Phe Asp Ser Gly Asn Leu Asp
305 310 315 320
Phe Ala Thr Ala Thr Ser Ile Arg Phe Ser Pro Asp Gly Phe Lys Arg
325 330 335
Phe Tyr Asp Asn Trp Glu Gln Tyr Ala Gly Lys Leu Leu Leu Arg Ser
340 345 350
Gln Gln Val Ser Asn Ser Pro Glu Ile Ile Arg Arg Leu Gly Cys Ile
355 360 365
Gly Met Asn Thr Pro Val Glu Val Asp Ile Tyr Ala His Ala Asn Ser
370 375 380
Thr Cys Val Met Gly Ser Arg Met Leu Asn Gly Leu Gly Gly Ser Ala
385 390 395 400
Asp Phe Leu Arg Asn Ser Lys Tyr Ser Ile Met His Thr Pro Ser Ala
405 410 415
Arg Pro Thr Lys Thr Asp Pro Thr Gly Val Ser Cys Ile Val Pro Phe
420 425 430
Ala Thr His Ile Asp Gln Thr Glu His Asp Leu Asp Val Val Val Thr
435 440 445
Glu Gln Gly Leu Ala Asp Val Arg Gly Leu Ser Pro Arg Glu Arg Ala
450 455 460
Arg Val Ile Ile Lys Asn Cys Ser His Pro Asp Tyr Thr Pro Ile Leu
465 470 475 480
Thr Asp Tyr Leu Asp Arg Ala Glu Phe Glu Cys Leu Arg Lys Gly Met
485 490 495
Gly His Glu Pro His Met Leu Phe Gln Ala Phe Asn Met His Lys Asn
500 505 510
Leu Gln Glu Lys Gly Thr Met Lys Ile Asp Ser Trp Glu
515 520 525
<210> 70
<211> 253
<212> PRT
<213> Penicillium Chrysogenum
<400> 70
Met Asp Lys Tyr Thr Ile Pro Leu Glu Lys Ala Val Glu Leu Thr Pro
1 5 10 15
Ile Pro Ser Glu Pro Asp Thr Phe Thr Asn Ala Gln Pro Leu Trp Ser
20 25 30
Phe Ala Gly Gly Tyr Gly Val Tyr Gly Gly Ser Thr Val Ala His Cys
35 40 45
Leu Val Ala Ala Gln Lys Thr Val Pro Cys Asp Tyr Val Ala His Ser
50 55 60
Leu His Cys Gly Phe Val Ser Pro Gly Asn Pro Lys Arg Pro Ile Glu
65 70 75 80
Tyr Arg Val Glu Arg Thr Arg Asp Gly Lys Ser Phe Ile Thr Arg Thr
85 90 95
Val His Ala Thr Gln Lys Ala Gly Val Ile Ser Glu Ala Ile Val Asn
100 105 110
Phe Val Arg Val Gly Thr Tyr Ser Asn Asn Ala Ser His Asp Leu Val
115 120 125
Asn Thr Gln Ile Arg Tyr Trp Val Arg Ala Lys Asp Pro Ile Gln Gln
130 135 140
Arg Ser Pro Gln Ala Gln Leu Ala Ala Leu Ala Tyr Met Ser Asp Ala
145 150 155 160
Tyr Leu Ile Gly Ala Ala Val Gln Val His Asp Val Ala Gly Gln Ala
165 170 175
Phe Gly Thr Lys Met Ala Met Ala Ala Ser Leu Asn His Thr Ile Tyr
180 185 190
Phe His Asn Pro Glu Ala Val Arg Ala Asp Glu Trp Met Cys Ser Glu
195 200 205
Arg Glu Ser Pro Trp Ala Gly Asn Asp Arg Ala Leu Val Val Gln Arg
210 215 220
Val Trp Ser Pro Glu Gly Ile Leu Val Ala Thr Cys Val Gln Glu Gly
225 230 235 240
Val Leu Arg Val Gln Val Pro Ser Glu Lys Val Lys Leu
245 250
<210> 71
<211> 462
<212> PRT
<213> Penicillium Chrysogenum
<400> 71
Met Phe Gly Leu Ser Arg Arg Ser Ala Leu Ala Arg Ser Lys Ser Leu
1 5 10 15
Ala Ala Leu Pro Ala Arg Ala Ala Val Ala Ala Gln Pro Ser Cys Ile
20 25 30
Asn Thr Gln Gly Phe His Ser Thr Pro Ser Gln Ala Ala Ser Arg Pro
35 40 45
Thr Trp Met Pro Met Arg Val Lys Thr Pro Trp Ile Glu Ala Leu Thr
50 55 60
Gln Ile Arg Glu Ala Gln Lys Ala Gly Pro Gln Glu Asn Ala Gly Gln
65 70 75 80
Ser Val Lys Arg Asp Leu Ser Pro Lys Lys Met Ser Asp Ser Tyr Tyr
85 90 95
Ser Ala Ile Leu Pro Leu Ala Gln Asp Lys Trp Leu Leu Asp Ser Tyr
100 105 110
Leu Asn Ala Ser Gly His Ile Arg Leu Gly Ser Leu Leu Met Asp Leu
115 120 125
Asp Ala Leu Ala Gly Ile Val Ala Tyr Arg His Thr Gly Asp Gly Val
130 135 140
Ser Thr Val Thr Ala Ala Cys Asp Arg Ile Thr Ile Glu Asn Pro Leu
145 150 155 160
Met Glu Ile Cys Asp Leu Glu Leu Ser Gly Gln Cys Thr Tyr Ala Thr
165 170 175
Gly Arg Ser Ser Met Glu Ile Ser Leu Gln Val Thr Lys Ala Arg Pro
180 185 190
Glu Gly Gln Glu Ala Lys Pro Glu Asp Ile Leu Ile Thr Cys Ala Phe
195 200 205
Thr Met Val Ser Leu Asp Pro Ala Thr Lys Ala Pro Val Pro Val Ala
210 215 220
Pro Leu Ile Val Glu Thr Glu Glu Glu Lys Arg Leu Phe Gln Lys Gly
225 230 235 240
Glu Ala Asn Tyr Gln Ala Lys Lys Ala Leu Arg Thr Arg Ser Leu Leu
245 250 255
Gln Lys Ser Pro Asp Asp Glu Glu Ser Asn Leu Ile His Ser Met Trp
260 265 270
Thr Lys Glu Met Ser Tyr Leu Asn Pro Gln Asn Pro Ala Thr Arg Pro
275 280 285
Ala Asn Gln Val Phe Met Ser Asp Thr Val Leu Lys Ser Ala Met Ile
290 295 300
Met Gln Pro Gln Asp Arg Asn Arg His Asn Phe Met Ile Phe Gly Gly
305 310 315 320
Phe Leu Leu Lys Gln Thr Phe Glu Leu Ala Phe Cys Cys Ala Ala Ser
325 330 335
Phe Ala His Ala Arg Pro Asn Phe Val Ala Leu Asp Pro Ser Thr Phe
340 345 350
Glu Asn Pro Val Pro Val Gly Ser Val Leu Tyr Leu Arg Ala Thr Val
355 360 365
Ser Tyr Thr Glu Pro Glu Glu Arg Glu Gly Asp Ser Thr Lys Tyr Thr
370 375 380
Lys Val Gln Val Arg Val Asp Ser Lys Val Arg Asp Val Glu His Gly
385 390 395 400
Thr Lys Lys Ser Thr Gly Met Phe Asn Tyr Thr Phe Leu Val Glu Lys
405 410 415
Asp Val Gln Val Met Pro Lys Gly Tyr Gly Glu Phe Met Leu Trp Ala
420 425 430
Asp Ala Arg Arg Arg Ala Gln Asn Ala Ala Ala Ile Asp Pro Ala His
435 440 445
Lys Met Ser Ala Leu Arg Ser Ile Lys Asp Ser Val Thr Glu
450 455 460
<210> 72
<211> 357
<212> PRT
<213> Penicillium Chrysogenum
<400> 72
Met Ser Pro Gln Arg Gly Ala Pro Asp Asn Asp Asp Leu Thr Ser Pro
1 5 10 15
His Ser Phe Val Glu Leu Met Ser Leu Gln Arg Leu Glu Asp Ala Leu
20 25 30
Ile Thr Tyr Pro Asp Ser Ala Lys Pro Glu Lys Ile Glu Arg Phe Arg
35 40 45
Ser Leu Ala Thr Pro Tyr Asn Pro Gly Gln Gly Thr Arg Ser Phe Gly
50 55 60
Gly His Val Tyr Ala Gln Ser Ala Tyr Ala Ala Ser Lys Thr Val Gly
65 70 75 80
Pro Gly Leu Val Ile His Asp Met Thr Gly Thr Phe Ile Leu Gly Gly
85 90 95
Leu Leu Asp Thr Pro Tyr Val Tyr Thr Val Arg His Ile Arg Asp Gly
100 105 110
Tyr Met Tyr Ser Thr Arg Ala Val Asp Ala Arg Gln Ala Gly Arg Ile
115 120 125
Cys Phe Ser Cys Ile Cys Ser Phe Lys Arg Asp Glu Lys Gln Arg Leu
130 135 140
Phe Gln His Gln Pro Ala Ser Ala Gln Ala Arg Phe Asp Ser Ile Leu
145 150 155 160
Ser Ala Lys Arg Pro Glu Asp Gln Glu Pro Ser Pro Ser Val Asp Ala
165 170 175
Glu Trp Trp Ile Glu Ala Val Arg Gln Gly Asn Ile Ser Glu Ser Glu
180 185 190
Phe Pro Gly Leu Asp Val Arg Lys Thr Asp Met Lys Asp Tyr Asn Cys
195 200 205
Ala Glu Asp Val Lys Gln His Pro Glu Arg Tyr Arg Gln Leu Thr Gln
210 215 220
Tyr Arg Leu Lys Gly Ser Pro Glu Glu Asp Pro Ala Ala Ser Leu Ala
225 230 235 240
Gln Ile Arg Glu Arg Glu Glu Asn Gly Glu Tyr Asp Asn Leu Tyr Ala
245 250 255
Cys Ala His Met Tyr Ser Ser Asp Lys Asn Ser Leu Leu Leu Ile Pro
260 265 270
Arg Ala Leu Gly Ile Lys Asn Trp Thr Glu Met Ala Ser Leu Thr Leu
275 280 285
Thr Val Ile Val His Gln His Gly Glu Ala Leu Arg Met Val Asn Trp
290 295 300
Asp Ser Ile Gly Asp Ser Asp Val Gly Val Asp Lys Leu Pro Met Lys
305 310 315 320
Trp Phe Val Gln Glu Gly Trp Thr Pro Arg Ala Thr Glu Asn Arg Gly
325 330 335
Thr His Glu Ser His Leu Trp Ser Pro Asp Gly Thr Leu Leu Ala Thr
340 345 350
Ser Leu Gln Asp Asn
355
<210> 73
<211> 320
<212> PRT
<213> Mus musculus
<400> 73
Met Ser Ala Pro Glu Gly Leu Gly Asp Ala His Gly Asp Ala Asp Arg
1 5 10 15
Gly Asp Leu Ser Gly Asp Leu Arg Ser Val Leu Val Thr Ser Val Leu
20 25 30
Asn Leu Glu Pro Leu Asp Glu Asp Leu Tyr Arg Gly Arg His Tyr Trp
35 40 45
Val Pro Thr Ser Gln Arg Leu Phe Gly Gly Gln Ile Met Gly Gln Ala
50 55 60
Leu Val Ala Ala Ala Lys Ser Val Ser Glu Asp Val His Val His Ser
65 70 75 80
Leu His Cys Tyr Phe Val Arg Ala Gly Asp Pro Lys Val Pro Val Leu
85 90 95
Tyr His Val Glu Arg Ile Arg Thr Gly Ala Ser Phe Ser Val Arg Ala
100 105 110
Val Lys Ala Val Gln His Gly Lys Ala Ile Phe Ile Cys Gln Ala Ser
115 120 125
Phe Gln Gln Met Gln Pro Ser Pro Leu Gln His Gln Phe Ser Met Pro
130 135 140
Ser Val Pro Pro Pro Glu Asp Leu Leu Asp His Glu Ala Leu Ile Asp
145 150 155 160
Gln Tyr Leu Arg Asp Pro Asn Leu His Lys Lys Tyr Arg Val Gly Leu
165 170 175
Asn Arg Val Ala Ala Gln Glu Val Pro Ile Glu Ile Lys Val Val Asn
180 185 190
Pro Pro Thr Leu Thr Gln Leu Gln Ala Leu Glu Pro Lys Gln Met Phe
195 200 205
Trp Val Arg Ala Arg Gly Tyr Ile Gly Glu Gly Asp Ile Lys Met His
210 215 220
Cys Cys Val Ala Ala Tyr Ile Ser Asp Tyr Ala Phe Leu Gly Thr Ala
225 230 235 240
Leu Leu Pro His Gln Ser Lys Tyr Lys Val Asn Phe Met Ala Ser Leu
245 250 255
Asp His Ser Met Trp Phe His Ala Pro Phe Arg Ala Asp His Trp Met
260 265 270
Leu Tyr Glu Cys Glu Ser Pro Trp Ala Gly Gly Phe Arg Gly Leu Val
275 280 285
His Gly Arg Leu Trp Arg Arg Asp Gly Val Leu Ala Val Thr Cys Ala
290 295 300
Gln Glu Gly Val Ile Arg Leu Lys Pro Gln Val Ser Glu Ser Lys Leu
305 310 315 320
<210> 74
<211> 472
<212> PRT
<213> Clostridium kluyveri
<400> 74
Met Ser Asn Glu Val Ser Ile Lys Glu Leu Ile Glu Lys Ala Lys Ala
1 5 10 15
Ala Gln Lys Lys Leu Glu Ala Tyr Ser Gln Glu Gln Val Asp Val Leu
20 25 30
Val Lys Ala Leu Gly Lys Val Val Tyr Asp Asn Ala Glu Met Phe Ala
35 40 45
Lys Glu Ala Val Glu Glu Thr Glu Met Gly Val Tyr Glu Asp Lys Val
50 55 60
Ala Lys Cys His Leu Lys Ser Gly Ala Ile Trp Asn His Ile Lys Asp
65 70 75 80
Lys Lys Thr Val Gly Ile Ile Lys Glu Glu Pro Glu Arg Ala Leu Val
85 90 95
Tyr Val Ala Lys Pro Lys Gly Val Val Ala Ala Thr Thr Pro Ile Thr
100 105 110
Asn Pro Val Val Thr Pro Met Cys Asn Ala Met Ala Ala Ile Lys Gly
115 120 125
Arg Asn Thr Ile Ile Val Ala Pro His Pro Lys Ala Lys Lys Val Ser
130 135 140
Ala His Thr Val Glu Leu Met Asn Ala Glu Leu Lys Lys Leu Gly Ala
145 150 155 160
Pro Glu Asn Ile Ile Gln Ile Val Glu Ala Pro Ser Arg Glu Ala Ala
165 170 175
Lys Glu Leu Met Glu Ser Ala Asp Val Val Ile Ala Thr Gly Gly Ala
180 185 190
Gly Arg Val Lys Ala Ala Tyr Ser Ser Gly Arg Pro Ala Tyr Gly Val
195 200 205
Gly Pro Gly Asn Ser Gln Val Ile Val Asp Lys Gly Tyr Asp Tyr Asn
210 215 220
Lys Ala Ala Gln Asp Ile Ile Thr Gly Arg Lys Tyr Asp Asn Gly Ile
225 230 235 240
Ile Cys Ser Ser Glu Gln Ser Val Ile Ala Pro Ala Glu Asp Tyr Asp
245 250 255
Lys Val Ile Ala Ala Phe Val Glu Asn Gly Ala Phe Tyr Val Glu Asp
260 265 270
Glu Glu Thr Val Glu Lys Phe Arg Ser Thr Leu Phe Lys Asp Gly Lys
275 280 285
Ile Asn Ser Lys Ile Ile Gly Lys Ser Val Gln Ile Ile Ala Asp Leu
290 295 300
Ala Gly Val Lys Val Pro Glu Gly Thr Lys Val Ile Val Leu Lys Gly
305 310 315 320
Lys Gly Ala Gly Glu Lys Asp Val Leu Cys Lys Glu Lys Met Cys Pro
325 330 335
Val Leu Val Ala Leu Lys Tyr Asp Thr Phe Glu Glu Ala Val Glu Ile
340 345 350
Ala Met Ala Asn Tyr Met Tyr Glu Gly Ala Gly His Thr Ala Gly Ile
355 360 365
His Ser Asp Asn Asp Glu Asn Ile Arg Tyr Ala Arg Thr Val Leu Pro
370 375 380
Ile Ser Arg Leu Val Val Asn Gln Pro Ala Thr Thr Ala Gly Gly Thr
385 390 395 400
Val Leu Pro Ile Ser Arg Leu Val Val Asn Gln Pro Ala Thr Thr Ala
405 410 415
Gly Gly Ser Phe Asn Asn Gly Phe Asn Pro Thr Thr Thr Leu Gly Cys
420 425 430
Gly Ser Trp Gly Arg Asn Ser Ile Ser Glu Asn Leu Thr Tyr Glu His
435 440 445
Leu Ile Asn Val Ser Arg Ile Gly Tyr Phe Asn Lys Glu Ala Lys Val
450 455 460
Pro Ser Tyr Glu Glu Ile Trp Gly
465 470
<210> 75
<211> 451
<212> PRT
<213> Porphyromonas gingivalis
<400> 75
Met Glu Ile Lys Glu Met Val Ser Leu Ala Arg Lys Ala Gln Lys Glu
1 5 10 15
Tyr Gln Ala Thr His Asn Gln Glu Ala Val Asp Asn Ile Cys Arg Ala
20 25 30
Ala Ala Lys Val Ile Tyr Glu Asn Ala Ala Ile Leu Ala Arg Glu Ala
35 40 45
Val Asp Glu Thr Gly Met Gly Val Tyr Glu His Lys Val Ala Lys Asn
50 55 60
Gln Gly Lys Ser Lys Gly Val Trp Tyr Asn Leu His Asn Lys Lys Ser
65 70 75 80
Ile Gly Ile Leu Asn Ile Asp Glu Arg Thr Gly Met Ile Glu Ile Ala
85 90 95
Lys Pro Ile Gly Val Val Gly Ala Val Thr Pro Thr Thr Asn Pro Ile
100 105 110
Val Thr Pro Met Ser Asn Ile Ile Phe Ala Leu Lys Thr Cys Asn Ala
115 120 125
Ile Ile Ile Ala Pro His Pro Arg Ser Lys Lys Cys Ser Ala His Ala
130 135 140
Val Arg Leu Ile Lys Glu Ala Ile Ala Pro Phe Asn Val Pro Glu Gly
145 150 155 160
Met Val Gln Ile Ile Glu Glu Pro Ser Ile Glu Lys Thr Gln Glu Leu
165 170 175
Met Gly Ala Val Asp Val Val Val Ala Thr Gly Gly Met Gly Met Val
180 185 190
Lys Ser Ala Tyr Ser Ser Gly Lys Pro Ser Phe Gly Val Gly Ala Gly
195 200 205
Asn Val Gln Val Ile Val Asp Ser Asn Ile Asp Phe Glu Ala Ala Ala
210 215 220
Glu Lys Ile Ile Thr Gly Arg Ala Phe Asp Asn Gly Ile Ile Cys Ser
225 230 235 240
Gly Glu Gln Ser Ile Ile Tyr Asn Glu Ala Asp Lys Glu Ala Val Phe
245 250 255
Thr Ala Phe Arg Asn His Gly Ala Tyr Phe Cys Asp Glu Ala Glu Gly
260 265 270
Asp Arg Ala Arg Ala Ala Ile Phe Glu Asn Gly Ala Ile Ala Lys Asp
275 280 285
Val Val Gly Gln Ser Val Ala Phe Ile Ala Lys Lys Ala Asn Ile Asn
290 295 300
Ile Pro Glu Gly Thr Arg Ile Leu Val Val Glu Ala Arg Gly Val Gly
305 310 315 320
Ala Glu Asp Val Ile Cys Lys Glu Lys Met Cys Pro Val Met Cys Ala
325 330 335
Leu Ser Tyr Lys His Phe Glu Glu Gly Val Glu Ile Ala Arg Thr Asn
340 345 350
Leu Ala Asn Glu Gly Asn Gly His Thr Cys Ala Ile His Ser Asn Asn
355 360 365
Gln Ala His Ile Ile Leu Ala Gly Ser Glu Leu Thr Val Ser Arg Ile
370 375 380
Val Val Asn Ala Pro Ser Ala Thr Thr Ala Gly Gly His Ile Gln Asn
385 390 395 400
Gly Leu Ala Val Thr Asn Thr Leu Gly Cys Gly Ser Trp Gly Asn Asn
405 410 415
Ser Ile Ser Glu Asn Phe Thr Tyr Lys His Leu Leu Asn Ile Ser Arg
420 425 430
Ile Ala Pro Leu Asn Ser Ser Ile His Ile Pro Asp Asp Lys Glu Ile
435 440 445
Trp Glu Leu
450
<210> 76
<211> 876
<212> PRT
<213> Clostridium kluyveri
<400> 76
Met Lys Val Thr Asn Val Glu Glu Leu Met Lys Lys Leu Gln Lys Val
1 5 10 15
Arg Gln Ala Gln Glu Lys Phe Ser Thr Tyr Thr Gln Glu Gln Val Asp
20 25 30
Glu Ile Phe Lys Gln Ala Ala Met Ala Ala Asn Ser Ala Arg Ile Lys
35 40 45
Leu Ala Lys Met Ala Val Glu Glu Thr Gly Met Gly Ile Val Glu Asp
50 55 60
Lys Val Ile Lys Asn His Phe Val Ser Glu Tyr Val Tyr Asn Lys Tyr
65 70 75 80
Lys Asp Glu Gln Thr Cys Gly Val Leu Glu Arg Asp Glu Gly Phe Gly
85 90 95
Met Val Arg Ile Ala Glu Pro Val Gly Val Ile Ala Ala Val Val Pro
100 105 110
Thr Thr Asn Pro Thr Ser Thr Ala Ile Phe Lys Ala Leu Ile Ala Leu
115 120 125
Lys Thr Arg Asn Gly Ile Val Phe Ser Pro His Pro Arg Ala Lys Arg
130 135 140
Ser Thr Val Ala Ala Ala Lys Ile Val Leu Asp Ala Ala Val Glu Ala
145 150 155 160
Gly Ala Pro Val Glu Ile Ile Gly Trp Ile Asp Glu Pro Ser Ile Glu
165 170 175
Leu Ser Gln Leu Val Met Lys Glu Ala Asp Leu Ile Leu Ala Thr Gly
180 185 190
Gly Pro Gly Met Val Arg Ala Ala Tyr Ser Ser Gly Lys Pro Ala Ile
195 200 205
Gly Val Gly Pro Gly Asn Thr Pro Val Ile Ile Asp Asp Ser Ala Asn
210 215 220
Ile Lys Met Ala Val Asn Ser Ile Leu Leu Ser Lys Ser Phe Asp His
225 230 235 240
Gly Val Ile Cys Ala Ser Glu Gln Ser Val Met Val Leu Asp Arg Ile
245 250 255
Tyr Glu Glu Val Lys Thr Glu Phe Ser Glu Arg Gly Gly Tyr Ile Leu
260 265 270
Lys Asn Asp Glu Ile Asp Lys Val Arg Glu Thr Ile Leu Val Asn Gly
275 280 285
Ser Ile Ser Pro Asp Ile Val Gly Gln Ser Ala Arg Lys Ile Ala Glu
290 295 300
Met Ala Gly Val Lys Val Pro Glu Arg Thr Lys Ile Leu Ile Gly Glu
305 310 315 320
Val Glu Ser Val Glu Leu Glu Glu Pro Phe Ser Arg Glu Lys Leu Ser
325 330 335
Pro Val Leu Ala Met Tyr Arg Val Lys Asn Phe Asn Asp Gly Leu Lys
340 345 350
Lys Ala Ala Arg Leu Val Glu Leu Gly Gly Ile Gly His Thr Ser Val
355 360 365
Leu Tyr Val Asn Thr Met Thr Gln Lys Glu Lys Val Glu Lys Phe Ser
370 375 380
Glu Ala Met Lys Thr Gly Arg Thr Leu Ile Asn Met Pro Ser Ala Gln
385 390 395 400
Gly Ala Ile Gly Asp Leu Tyr Asn Phe Lys Leu Ala Pro Ser Leu Thr
405 410 415
Leu Gly Cys Gly Ser Trp Gly Gly Asn Ser Ile Ser Glu Asn Val Gly
420 425 430
Pro Lys His Leu Leu Asn Ile Lys Ser Ile Ala Glu Arg Arg Glu Asn
435 440 445
Met Leu Trp Phe Arg Val Pro Glu Lys Ile Tyr Phe Lys Tyr Gly Ala
450 455 460
Leu Gly Thr Ala Leu Lys Glu Leu Lys Thr Met His Lys Lys Lys Val
465 470 475 480
Phe Ile Val Thr Asp Lys Val Leu Tyr Gln Leu Gly Tyr Thr Asp Ser
485 490 495
Ile Thr Lys Ile Leu Gly Glu Met Gly Val Gly Tyr Lys Ile Phe Thr
500 505 510
Asp Val Glu Pro Asp Pro Thr Leu Ala Thr Ala Lys Lys Gly Ser Ala
515 520 525
Glu Met Leu Gln Tyr Asn Pro Asp Thr Ile Ile Ser Val Gly Gly Gly
530 535 540
Ser Ala Met Asp Ala Ala Lys Ile Met Trp Val Met Tyr Glu His Pro
545 550 555 560
Glu Ile Lys Phe Glu Asp Leu Ala Met Arg Phe Met Asp Ile Arg Lys
565 570 575
Arg Val Tyr Thr Phe Pro Lys Met Gly Glu Lys Ser Met Met Ile Ser
580 585 590
Val Ala Thr Ser Ala Gly Thr Gly Ser Glu Val Thr Pro Phe Thr Val
595 600 605
Ile Thr Asp Glu Lys Thr Gly Ala Lys Tyr Pro Leu Ala Asp Tyr Glu
610 615 620
Leu Thr Pro Asp Ile Ala Ile Ile Asp Ala Glu Leu Met Met Asn Met
625 630 635 640
Pro Lys Gly Leu Thr Ala Ala Ser Gly Ile Asp Ala Met Thr His Ala
645 650 655
Leu Glu Ser Tyr Val Ser Val Met Ala Ser Glu Tyr Thr Asn Gly Leu
660 665 670
Ala Leu Glu Ala Ile Arg Leu Ile Phe Lys Tyr Leu Pro Leu Ala Tyr
675 680 685
Ala Glu Gly Thr Thr Asn Val Lys Ala Arg Glu Lys Met Ala His Ala
690 695 700
Ser Thr Met Ala Gly Met Ala Phe Ala Asn Ala Phe Leu Gly Val Cys
705 710 715 720
His Ser Met Ala His Lys Leu Gly Ala Ala His His Ile Pro His Gly
725 730 735
Ile Ala Asn Ala Leu Met Ile Asp Glu Val Ile Lys Phe Asn Ala Val
740 745 750
Asn Asp Pro Arg Lys Gln Ala Ile Phe Pro Gln Tyr Lys Tyr Pro Asn
755 760 765
Ala Lys Trp Arg Tyr Ala Arg Ile Ala Asp Tyr Leu Glu Leu Gly Gly
770 775 780
Asn Ser Glu Asp Glu Lys Val Gly Leu Leu Ile Lys Ala Ile Asp Glu
785 790 795 800
Leu Lys Glu Lys Ile Asn Ile Pro Lys Thr Ile Arg Glu Ala Gly Val
805 810 815
Ala Glu Lys Lys Phe Tyr Ala Thr Leu Asp Asp Met Ser Glu Gln Ala
820 825 830
Phe Asp Asp Gln Cys Thr Gly Ala Asn Pro Arg Tyr Pro Leu Ile Ser
835 840 845
Glu Ile Lys Gln Met Tyr Ile Asn Ala Phe Asp Lys Val Ser Pro Val
850 855 860
Val Asp Glu Glu Glu Lys Leu Lys Val Ser Leu Leu
865 870 875
<210> 77
<211> 491
<212> PRT
<213> Clostridium kluyveri
<400> 77
Met Glu Ile Met Asp Lys Asp Leu Gln Ser Ile Gln Glu Val Arg Thr
1 5 10 15
Leu Ile Ala Lys Ala Lys Lys Ala Gln Ala Glu Phe Lys Asn Phe Ser
20 25 30
Gln Glu Ala Val Asn Lys Val Ile Glu Lys Ile Ala Lys Ala Thr Glu
35 40 45
Val Glu Ala Val Lys Leu Ala Lys Leu Ala Tyr Glu Asp Thr Gly Tyr
50 55 60
Gly Lys Trp Glu Asp Lys Val Ile Lys Asn Lys Phe Ser Ser Ile Val
65 70 75 80
Val Tyr Asn Tyr Ile Lys Asp Leu Lys Thr Val Gly Ile Leu Lys Glu
85 90 95
Asp Lys Glu Lys Lys Leu Ile Asp Ile Ala Val Pro Leu Gly Val Ile
100 105 110
Ala Gly Leu Ile Pro Ser Thr Asn Pro Thr Ser Thr Ala Ile Phe Lys
115 120 125
Val Leu Ile Ala Leu Lys Ala Gly Asn Ala Ile Val Phe Ser Pro His
130 135 140
Pro Thr Ala Val Arg Ser Ile Thr Glu Thr Val Lys Ile Met Gln Lys
145 150 155 160
Ala Ala Val Glu Ala Gly Ala Pro Asp Gly Leu Ile Gln Cys Met Ser
165 170 175
Ile Leu Thr Val Glu Gly Thr Ala Glu Leu Met Lys Asn Lys Asp Thr
180 185 190
Ala Leu Ile Leu Ala Thr Gly Gly Glu Gly Met Val Arg Ala Ala Tyr
195 200 205
Ser Ser Gly Thr Pro Ala Ile Gly Val Gly Pro Gly Asn Gly Pro Cys
210 215 220
Phe Ile Glu Arg Thr Ala Asp Ile Pro Thr Ala Val Arg Lys Val Ile
225 230 235 240
Gly Ser Asp Thr Phe Asp Asn Gly Val Ile Cys Ala Ser Glu Gln Ser
245 250 255
Ile Ile Ala Glu Thr Val Lys Lys Ala Glu Ile Ile Glu Glu Phe Lys
260 265 270
Arg Gln Lys Gly Tyr Phe Leu Asn Ala Glu Glu Ser Glu Lys Val Gly
275 280 285
Lys Ile Leu Leu Arg Ala Asn Gly Thr Pro Asn Pro Ala Ile Val Gly
290 295 300
Lys Asp Val Gln Ala Leu Ala Lys Leu Ala Gly Ile Ser Ile Pro Ser
305 310 315 320
Asp Ala Val Ile Leu Leu Ser Glu Gln Thr Asp Val Ser Pro Lys Asn
325 330 335
Pro Tyr Ala Lys Glu Lys Leu Ala Pro Val Leu Ala Phe Tyr Thr Val
340 345 350
Glu Asp Trp His Glu Ala Cys Glu Lys Ser Leu Ala Leu Leu His Asn
355 360 365
Gln Gly Ser Gly His Thr Leu Ile Ile His Ser Gln Asn Glu Glu Ile
370 375 380
Ile Arg Glu Phe Ala Leu Lys Lys Pro Val Ser Arg Ile Leu Val Asn
385 390 395 400
Ser Pro Gly Ser Leu Gly Gly Ile Gly Gly Ala Thr Asn Leu Val Pro
405 410 415
Ser Leu Thr Leu Gly Cys Gly Ala Val Gly Gly Ser Ala Thr Ser Asp
420 425 430
Asn Val Gly Pro Glu Asn Leu Phe Asn Ile Arg Lys Val Ala Tyr Gly
435 440 445
Thr Thr Thr Val Glu Glu Ile Arg Glu Ala Phe Gly Val Gly Ala Ala
450 455 460
Ser Ser Ser Ala Pro Ala Glu Pro Glu Asp Asn Glu Asp Val Gln Ala
465 470 475 480
Ile Val Lys Ala Ile Met Ala Lys Leu Asn Leu
485 490
<210> 78
<211> 491
<212> PRT
<213> Clostridium kluyveri
<400> 78
Met Glu Ile Met Asp Lys Asp Leu Gln Ser Ile Gln Glu Val Arg Thr
1 5 10 15
Leu Ile Ala Lys Ala Lys Lys Ala Gln Ala Glu Phe Lys Asn Phe Ser
20 25 30
Gln Glu Ala Val Asn Lys Val Ile Glu Lys Ile Ala Lys Ala Thr Glu
35 40 45
Val Glu Ala Val Lys Leu Ala Lys Leu Ala Tyr Glu Asp Thr Gly Tyr
50 55 60
Gly Lys Trp Glu Asp Lys Val Ile Lys Asn Lys Phe Ser Ser Ile Val
65 70 75 80
Val Tyr Asn Tyr Ile Lys Asp Leu Lys Thr Val Gly Ile Leu Lys Glu
85 90 95
Asp Lys Glu Lys Lys Leu Ile Asp Ile Ala Val Pro Leu Gly Val Ile
100 105 110
Ala Gly Leu Ile Pro Ser Thr Asn Pro Thr Ser Thr Ala Ile Phe Lys
115 120 125
Val Leu Ile Ala Leu Lys Ala Gly Asn Ala Ile Val Phe Ser Pro His
130 135 140
Pro Thr Ala Val Arg Ser Ile Thr Glu Thr Val Lys Ile Met Gln Lys
145 150 155 160
Ala Ala Val Glu Ala Gly Ala Pro Asp Gly Leu Ile Gln Cys Met Ser
165 170 175
Ile Leu Thr Val Glu Gly Thr Ala Glu Leu Met Lys Asn Lys Asp Thr
180 185 190
Ala Leu Ile Leu Ala Thr Gly Gly Glu Gly Met Val Arg Ala Ala Tyr
195 200 205
Ser Ser Gly Thr Pro Ala Ile Gly Val Gly Pro Gly Asn Gly Pro Cys
210 215 220
Phe Ile Glu Arg Thr Ala Asp Ile Pro Thr Ala Val Arg Lys Val Ile
225 230 235 240
Gly Ser Asp Thr Phe Asp Asn Gly Val Ile Cys Ala Ser Glu Gln Ser
245 250 255
Ile Ile Ala Glu Thr Val Lys Lys Ala Glu Ile Ile Glu Glu Phe Lys
260 265 270
Arg Gln Lys Gly Tyr Phe Leu Asn Ala Glu Glu Ser Glu Lys Val Gly
275 280 285
Lys Ile Leu Leu Arg Ala Asn Gly Thr Pro Asn Pro Ala Ile Val Gly
290 295 300
Lys Asp Val Gln Ala Leu Ala Lys Leu Ala Gly Ile Ser Ile Pro Ser
305 310 315 320
Asp Ala Val Ile Leu Leu Ser Glu Gln Thr Asp Val Ser Pro Lys Asn
325 330 335
Pro Tyr Ala Lys Glu Lys Leu Ala Pro Val Leu Ala Phe Tyr Thr Val
340 345 350
Glu Asp Trp His Glu Ala Cys Glu Lys Ser Leu Ala Leu Leu His Asn
355 360 365
Gln Gly Ser Gly His Thr Leu Ile Ile His Ser Gln Asn Glu Glu Ile
370 375 380
Ile Arg Glu Phe Ala Leu Lys Lys Pro Val Ser Arg Ile Leu Val Asn
385 390 395 400
Ser Pro Gly Ser Leu Gly Gly Ile Gly Gly Ala Thr Asn Leu Val Pro
405 410 415
Ser Leu Thr Leu Gly Cys Gly Ala Val Gly Gly Ser Ala Thr Ser Asp
420 425 430
Asn Val Gly Pro Glu Asn Leu Phe Asn Ile Arg Lys Val Ala Tyr Gly
435 440 445
Thr Thr Thr Val Glu Glu Ile Arg Glu Ala Phe Gly Val Gly Ala Ala
450 455 460
Ser Ser Ser Ala Pro Ala Glu Pro Glu Asp Asn Glu Asp Val Gln Ala
465 470 475 480
Ile Val Lys Ala Ile Met Ala Lys Leu Asn Leu
485 490
<210> 79
<211> 469
<212> PRT
<213> Propionibacterium freudenreichi
<400> 79
Met Thr Ile Ser Pro Glu Leu Ile Gln Gln Val Val Arg Glu Thr Val
1 5 10 15
Arg Glu Val Ile Ser Arg Gln Asp Ser Gly Thr Asp Ala Pro Ser Gly
20 25 30
Thr Asp Gly Ile Phe Thr Asp Met Asn Ser Ala Val Asp Ala Ala Asp
35 40 45
Val Ala Trp Arg Gln Tyr Met Asp Cys Ser Leu Arg Asp Arg Asn Arg
50 55 60
Phe Ile Gln Ala Ile Arg Asp Val Ala Ser Glu Pro Asp Asn Leu Glu
65 70 75 80
Tyr Met Ala Thr Ala Thr Val Glu Glu Thr Gly Met Gly Asn Val Pro
85 90 95
His Lys Ile Leu Lys Asn Arg Tyr Ala Ala Leu Tyr Thr Pro Gly Thr
100 105 110
Glu Asp Ile Ile Thr Glu Ala Trp Ser Gly Asp Asp Gly Leu Thr Thr
115 120 125
Val Glu Phe Ser Pro Phe Gly Val Ile Gly Ala Ile Thr Pro Thr Thr
130 135 140
Asn Pro Thr Glu Thr Val Ile Asn Asn Thr Ile Gly Met Leu Ala Ala
145 150 155 160
Gly Asn Ala Val Val Phe Ser Pro His Pro Arg Ala Lys Lys Ile Thr
165 170 175
Leu Trp Leu Val Arg Lys Ile Asn Arg Ala Leu Ala Ala Ala Gly Ala
180 185 190
Pro Ala Asn Leu Val Val Thr Val Glu Glu Pro Ser Ile Asp Asn Thr
195 200 205
Asn Ala Met Met Ser His Glu Lys Val Arg Met Leu Val Ala Thr Gly
210 215 220
Gly Pro Gly Ile Val Lys Ala Val Leu Ser Ser Gly Lys Lys Ala Ile
225 230 235 240
Gly Ala Gly Ala Gly Asn Pro Pro Ala Val Val Asp Asp Thr Ala Asp
245 250 255
Ile Ala Lys Ala Ala Arg Asp Ile Val Asp Gly Ala Ser Phe Asp Asn
260 265 270
Asn Leu Pro Cys Thr Ala Glu Lys Glu Val Leu Ala Val Asp Ser Ile
275 280 285
Ala Asp Leu Leu Lys Phe Glu Met Leu Lys His Gly Cys Phe Glu Leu
290 295 300
Lys Asp Arg Ala Val Met Asp Lys Leu Ala Ala Leu Val Thr Lys Gly
305 310 315 320
Gln His Ala Asn Ala Ala Tyr Val Gly Lys Pro Ala Ala Gln Leu Ala
325 330 335
Ser Glu Val Gly Leu Ser Ala Pro Lys Asp Thr Arg Leu Leu Ile Cys
340 345 350
Glu Val Pro Phe Asp His Pro Phe Val Gln Val Glu Leu Met Met Pro
355 360 365
Ile Leu Pro Ile Val Arg Met Pro Asp Val Asp Thr Ala Ile Asp Lys
370 375 380
Ala Val Glu Val Glu His Gly Asn Arg His Thr Ala Val Met His Ser
385 390 395 400
Ser Asn Val Asn Ala Leu Thr Lys Met Gly Lys Leu Ile Gln Thr Thr
405 410 415
Ile Phe Val Lys Asn Gly Pro Ser Tyr Asn Gly Ile Gly Ile Asp Gly
420 425 430
Glu Gly Phe Pro Thr Phe Thr Ile Ala Gly Pro Thr Gly Glu Gly Leu
435 440 445
Thr Ser Ala Arg Cys Phe Ala Arg Lys Arg Arg Cys Val Leu Lys Ser
450 455 460
Gly Leu Asn Ile Arg
465
<210> 80
<211> 858
<212> PRT
<213> Clostridium acetobutylicum
<400> 80
Met Lys Val Thr Asn Gln Lys Glu Leu Lys Gln Lys Leu Asn Glu Leu
1 5 10 15
Arg Glu Ala Gln Lys Lys Phe Ala Thr Tyr Thr Gln Glu Gln Val Asp
20 25 30
Lys Ile Phe Lys Gln Cys Ala Ile Ala Ala Ala Lys Glu Arg Ile Asn
35 40 45
Leu Ala Lys Leu Ala Val Glu Glu Thr Gly Ile Gly Leu Val Glu Asp
50 55 60
Lys Ile Ile Lys Asn His Phe Ala Ala Glu Tyr Ile Tyr Asn Lys Tyr
65 70 75 80
Lys Asn Glu Lys Thr Cys Gly Ile Ile Asp His Asp Asp Ser Leu Gly
85 90 95
Ile Thr Lys Val Ala Glu Pro Ile Gly Ile Val Ala Ala Ile Val Pro
100 105 110
Thr Thr Asn Pro Thr Ser Thr Ala Ile Phe Lys Ser Leu Ile Ser Leu
115 120 125
Lys Thr Arg Asn Ala Ile Phe Phe Ser Pro His Pro Arg Ala Lys Lys
130 135 140
Ser Thr Ile Ala Ala Ala Lys Leu Ile Leu Asp Ala Ala Val Lys Ala
145 150 155 160
Gly Ala Pro Lys Asn Ile Ile Gly Trp Ile Asp Glu Pro Ser Ile Glu
165 170 175
Leu Ser Gln Asp Leu Met Ser Glu Ala Asp Ile Ile Leu Ala Thr Gly
180 185 190
Gly Pro Ser Met Val Lys Ala Ala Tyr Ser Ser Gly Lys Pro Ala Ile
195 200 205
Gly Val Gly Ala Gly Asn Thr Pro Ala Ile Ile Asp Glu Ser Ala Asp
210 215 220
Ile Asp Met Ala Val Ser Ser Ile Ile Leu Ser Lys Thr Tyr Asp Asn
225 230 235 240
Gly Val Ile Cys Ala Ser Glu Gln Ser Ile Leu Val Met Asn Ser Ile
245 250 255
Tyr Glu Lys Val Lys Glu Glu Phe Val Lys Arg Gly Ser Tyr Ile Leu
260 265 270
Asn Gln Asn Glu Ile Ala Lys Ile Lys Glu Thr Met Phe Lys Asn Gly
275 280 285
Ala Ile Asn Ala Asp Ile Val Gly Lys Ser Ala Tyr Ile Ile Ala Lys
290 295 300
Met Ala Gly Ile Glu Val Pro Gln Thr Thr Lys Ile Leu Ile Gly Glu
305 310 315 320
Val Gln Ser Val Glu Lys Ser Glu Leu Phe Ser His Glu Lys Leu Ser
325 330 335
Pro Val Leu Ala Met Tyr Lys Val Lys Asp Phe Asp Glu Ala Leu Lys
340 345 350
Lys Ala Gln Arg Leu Ile Glu Leu Gly Gly Ser Gly His Thr Ser Ser
355 360 365
Leu Tyr Ile Asp Ser Gln Asn Asn Lys Asp Lys Val Lys Glu Phe Gly
370 375 380
Leu Ala Met Lys Thr Ser Arg Thr Phe Ile Asn Met Pro Ser Ser Gln
385 390 395 400
Gly Ala Ser Gly Asp Leu Tyr Asn Phe Ala Ile Ala Pro Ser Phe Thr
405 410 415
Leu Gly Cys Gly Thr Trp Gly Gly Asn Ser Val Ser Gln Asn Val Glu
420 425 430
Pro Lys His Leu Leu Asn Ile Lys Ser Val Ala Glu Arg Arg Glu Asn
435 440 445
Met Leu Trp Phe Lys Val Pro Gln Lys Ile Tyr Phe Lys Tyr Gly Cys
450 455 460
Leu Arg Phe Ala Leu Lys Glu Leu Lys Asp Met Asn Lys Lys Arg Ala
465 470 475 480
Phe Ile Val Thr Asp Lys Asp Leu Phe Lys Leu Gly Tyr Val Asn Lys
485 490 495
Ile Thr Lys Val Leu Asp Glu Ile Asp Ile Lys Tyr Ser Ile Phe Thr
500 505 510
Asp Ile Lys Ser Asp Pro Thr Ile Asp Ser Val Lys Lys Gly Ala Lys
515 520 525
Glu Met Leu Asn Phe Glu Pro Asp Thr Ile Ile Ser Ile Gly Gly Gly
530 535 540
Ser Pro Met Asp Ala Ala Lys Val Met His Leu Leu Tyr Glu Tyr Pro
545 550 555 560
Glu Ala Glu Ile Glu Asn Leu Ala Ile Asn Phe Met Asp Ile Arg Lys
565 570 575
Arg Ile Cys Asn Phe Pro Lys Leu Gly Thr Lys Ala Ile Ser Val Ala
580 585 590
Ile Pro Thr Thr Ala Gly Thr Gly Ser Glu Ala Thr Pro Phe Ala Val
595 600 605
Ile Thr Asn Asp Glu Thr Gly Met Lys Tyr Pro Leu Thr Ser Tyr Glu
610 615 620
Leu Thr Pro Asn Met Ala Ile Ile Asp Thr Glu Leu Met Leu Asn Met
625 630 635 640
Pro Arg Lys Leu Thr Ala Ala Thr Gly Ile Asp Ala Leu Val His Ala
645 650 655
Ile Glu Ala Tyr Val Ser Val Met Ala Thr Asp Tyr Thr Asp Glu Leu
660 665 670
Ala Leu Arg Ala Ile Lys Met Ile Phe Lys Tyr Leu Pro Arg Ala Tyr
675 680 685
Lys Asn Gly Thr Asn Asp Ile Glu Ala Arg Glu Lys Met Ala His Ala
690 695 700
Ser Asn Ile Ala Gly Met Ala Phe Ala Asn Ala Phe Leu Gly Val Cys
705 710 715 720
His Ser Met Ala His Lys Leu Gly Ala Met His His Val Pro His Gly
725 730 735
Ile Ala Cys Ala Val Leu Ile Glu Glu Val Ile Lys Tyr Asn Ala Thr
740 745 750
Asp Cys Pro Thr Lys Gln Thr Ala Phe Pro Gln Tyr Lys Ser Pro Asn
755 760 765
Ala Lys Arg Lys Tyr Ala Glu Ile Ala Glu Tyr Leu Asn Leu Lys Gly
770 775 780
Thr Ser Asp Thr Glu Lys Val Thr Ala Leu Ile Glu Ala Ile Ser Lys
785 790 795 800
Leu Lys Ile Asp Leu Ser Ile Pro Gln Asn Ile Ser Ala Ala Gly Ile
805 810 815
Asn Lys Lys Asp Phe Tyr Asn Thr Leu Asp Lys Met Ser Glu Leu Ala
820 825 830
Phe Asp Asp Gln Cys Thr Thr Ala Asn Pro Arg Tyr Pro Leu Ile Ser
835 840 845
Glu Leu Lys Asp Ile Tyr Ile Lys Ser Phe
850 855
<210> 81
<211> 862
<212> PRT
<213> Clostridium acetobutylicum
<400> 81
Met Lys Val Thr Thr Val Lys Glu Leu Asp Glu Lys Leu Lys Val Ile
1 5 10 15
Lys Glu Ala Gln Lys Lys Phe Ser Cys Tyr Ser Gln Glu Met Val Asp
20 25 30
Glu Ile Phe Arg Asn Ala Ala Met Ala Ala Ile Asp Ala Arg Ile Glu
35 40 45
Leu Ala Lys Ala Ala Val Leu Glu Thr Gly Met Gly Leu Val Glu Asp
50 55 60
Lys Val Ile Lys Asn His Phe Ala Gly Glu Tyr Ile Tyr Asn Lys Tyr
65 70 75 80
Lys Asp Glu Lys Thr Cys Gly Ile Ile Glu Arg Asn Glu Pro Tyr Gly
85 90 95
Ile Thr Lys Ile Ala Glu Pro Ile Gly Val Val Ala Ala Ile Ile Pro
100 105 110
Val Thr Asn Pro Thr Ser Thr Thr Ile Phe Lys Ser Leu Ile Ser Leu
115 120 125
Lys Thr Arg Asn Gly Ile Phe Phe Ser Pro His Pro Arg Ala Lys Lys
130 135 140
Ser Thr Ile Leu Ala Ala Lys Thr Ile Leu Asp Ala Ala Val Lys Ser
145 150 155 160
Gly Ala Pro Glu Asn Ile Ile Gly Trp Ile Asp Glu Pro Ser Ile Glu
165 170 175
Leu Thr Gln Tyr Leu Met Gln Lys Ala Asp Ile Thr Leu Ala Thr Gly
180 185 190
Gly Pro Ser Leu Val Lys Ser Ala Tyr Ser Ser Gly Lys Pro Ala Ile
195 200 205
Gly Val Gly Pro Gly Asn Thr Pro Val Ile Ile Asp Glu Ser Ala His
210 215 220
Ile Lys Met Ala Val Ser Ser Ile Ile Leu Ser Lys Thr Tyr Asp Asn
225 230 235 240
Gly Val Ile Cys Ala Ser Glu Gln Ser Val Ile Val Leu Lys Ser Ile
245 250 255
Tyr Asn Lys Val Lys Asp Glu Phe Gln Glu Arg Gly Ala Tyr Ile Ile
260 265 270
Lys Lys Asn Glu Leu Asp Lys Val Arg Glu Val Ile Phe Lys Asp Gly
275 280 285
Ser Val Asn Pro Lys Ile Val Gly Gln Ser Ala Tyr Thr Ile Ala Ala
290 295 300
Met Ala Gly Ile Lys Val Pro Lys Thr Thr Arg Ile Leu Ile Gly Glu
305 310 315 320
Val Thr Ser Leu Gly Glu Glu Glu Pro Phe Ala His Glu Lys Leu Ser
325 330 335
Pro Val Leu Ala Met Tyr Glu Ala Asp Asn Phe Asp Asp Ala Leu Lys
340 345 350
Lys Ala Val Thr Leu Ile Asn Leu Gly Gly Leu Gly His Thr Ser Gly
355 360 365
Ile Tyr Ala Asp Glu Ile Lys Ala Arg Asp Lys Ile Asp Arg Phe Ser
370 375 380
Ser Ala Met Lys Thr Val Arg Thr Phe Val Asn Ile Pro Thr Ser Gln
385 390 395 400
Gly Ala Ser Gly Asp Leu Tyr Asn Phe Arg Ile Pro Pro Ser Phe Thr
405 410 415
Leu Gly Cys Gly Phe Trp Gly Gly Asn Ser Val Ser Glu Asn Val Gly
420 425 430
Pro Lys His Leu Leu Asn Ile Lys Thr Val Ala Glu Arg Arg Glu Asn
435 440 445
Met Leu Trp Phe Arg Val Pro His Lys Val Tyr Phe Lys Phe Gly Cys
450 455 460
Leu Gln Phe Ala Leu Lys Asp Leu Lys Asp Leu Lys Lys Lys Arg Ala
465 470 475 480
Phe Ile Val Thr Asp Ser Asp Pro Tyr Asn Leu Asn Tyr Val Asp Ser
485 490 495
Ile Ile Lys Ile Leu Glu His Leu Asp Ile Asp Phe Lys Val Phe Asn
500 505 510
Lys Val Gly Arg Glu Ala Asp Leu Lys Thr Ile Lys Lys Ala Thr Glu
515 520 525
Glu Met Ser Ser Phe Met Pro Asp Thr Ile Ile Ala Leu Gly Gly Thr
530 535 540
Pro Glu Met Ser Ser Ala Lys Leu Met Trp Val Leu Tyr Glu His Pro
545 550 555 560
Glu Val Lys Phe Glu Asp Leu Ala Ile Lys Phe Met Asp Ile Arg Lys
565 570 575
Arg Ile Tyr Thr Phe Pro Lys Leu Gly Lys Lys Ala Met Leu Val Ala
580 585 590
Ile Thr Thr Ser Ala Gly Ser Gly Ser Glu Val Thr Pro Phe Ala Leu
595 600 605
Val Thr Asp Asn Asn Thr Gly Asn Lys Tyr Met Leu Ala Asp Tyr Glu
610 615 620
Met Thr Pro Asn Met Ala Ile Val Asp Ala Glu Leu Met Met Lys Met
625 630 635 640
Pro Lys Gly Leu Thr Ala Tyr Ser Gly Ile Asp Ala Leu Val Asn Ser
645 650 655
Ile Glu Ala Tyr Thr Ser Val Tyr Ala Ser Glu Tyr Thr Asn Gly Leu
660 665 670
Ala Leu Glu Ala Ile Arg Leu Ile Phe Lys Tyr Leu Pro Glu Ala Tyr
675 680 685
Lys Asn Gly Arg Thr Asn Glu Lys Ala Arg Glu Lys Met Ala His Ala
690 695 700
Ser Thr Met Ala Gly Met Ala Ser Ala Asn Ala Phe Leu Gly Leu Cys
705 710 715 720
His Ser Met Ala Ile Lys Leu Ser Ser Glu His Asn Ile Pro Ser Gly
725 730 735
Ile Ala Asn Ala Leu Leu Ile Glu Glu Val Ile Lys Phe Asn Ala Val
740 745 750
Asp Asn Pro Val Lys Gln Ala Pro Cys Pro Gln Tyr Lys Tyr Pro Asn
755 760 765
Thr Ile Phe Arg Tyr Ala Arg Ile Ala Asp Tyr Ile Lys Leu Gly Gly
770 775 780
Asn Thr Asp Glu Glu Lys Val Asp Leu Leu Ile Asn Lys Ile His Glu
785 790 795 800
Leu Lys Lys Ala Leu Asn Ile Pro Thr Ser Ile Lys Asp Ala Gly Val
805 810 815
Leu Glu Glu Asn Phe Tyr Ser Ser Leu Asp Arg Ile Ser Glu Leu Ala
820 825 830
Leu Asp Asp Gln Cys Thr Gly Ala Asn Pro Arg Phe Pro Leu Thr Ser
835 840 845
Glu Ile Lys Glu Met Tyr Ile Asn Cys Phe Lys Lys Gln Pro
850 855 860
<210> 82
<211> 453
<212> PRT
<213> Vibrio fluvialis
<400> 82
Met Asn Lys Pro Gln Ser Trp Glu Ala Arg Ala Glu Thr Tyr Ser Leu
1 5 10 15
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val
20 25 30
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val Asn Gly Arg Arg
35 40 45
Tyr Leu Asp Ala Asn Ser Gly Leu Trp Asn Met Val Ala Gly Phe Asp
50 55 60
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro
65 70 75 80
Gly Tyr His Ala Phe Phe Gly Arg Met Ser Asp Gln Thr Val Met Leu
85 90 95
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe
100 105 110
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu
115 120 125
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu
130 135 140
Thr Arg Trp Asn Ala Tyr His Gly Val Thr Ala Val Ser Ala Ser Met
145 150 155 160
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe
165 170 175
Val His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu
180 185 190
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr
195 200 205
Ile Gln Arg Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro
210 215 220
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln
225 230 235 240
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp
245 250 255
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val
260 265 270
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr
275 280 285
Ala Gly Phe Phe Pro Met Gly Ala Val Ile Leu Gly Pro Glu Leu Ser
290 295 300
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly
305 310 315 320
Phe Thr Ala Ser Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala
325 330 335
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu
340 345 350
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn
355 360 365
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val
370 375 380
Lys Asp Lys Ala Ser Lys Thr Pro Phe Asp Gly Asn Leu Ser Val Ser
385 390 395 400
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro
405 410 415
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala
420 425 430
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val
435 440 445
Phe Ala Glu Val Ala
450
<210> 83
<211> 449
<212> PRT
<213> Bacillus weihenstephanensis
<400> 83
Met Gln Ala Thr Glu Gln Thr Gln Ser Leu Lys Lys Thr Asp Glu Lys
1 5 10 15
Tyr Leu Trp His Ala Met Arg Gly Ala Ala Pro Ser Pro Thr Asn Leu
20 25 30
Ile Ile Thr Lys Ala Glu Gly Ala Trp Val Thr Asp Ile Asp Gly Asn
35 40 45
Arg Tyr Leu Asp Gly Met Ser Gly Leu Trp Cys Val Asn Val Gly Tyr
50 55 60
Gly Arg Lys Glu Leu Ala Arg Ala Ala Phe Glu Gln Leu Glu Glu Met
65 70 75 80
Pro Tyr Phe Pro Leu Thr Gln Ser His Val Pro Ala Ile Lys Leu Ala
85 90 95
Glu Lys Leu Asn Glu Trp Leu Asp Asp Glu Tyr Val Ile Phe Phe Ser
100 105 110
Asn Ser Gly Ser Glu Ala Asn Glu Thr Ala Phe Lys Ile Ala Arg Gln
115 120 125
Tyr His Gln Gln Lys Gly Asp His Gly Arg Tyr Lys Phe Ile Ser Arg
130 135 140
Tyr Arg Ala Tyr His Gly Asn Ser Met Gly Ala Leu Ala Ala Thr Gly
145 150 155 160
Gln Ala Gln Arg Lys Tyr Lys Tyr Glu Pro Leu Gly Gln Gly Phe Leu
165 170 175
His Val Ala Pro Pro Asp Thr Tyr Arg Asn Pro Glu Asp Val His Thr
180 185 190
Leu Ala Ser Ala Glu Glu Ile Asp Arg Val Met Thr Trp Glu Leu Ser
195 200 205
Gln Thr Val Ala Gly Val Ile Met Glu Pro Ile Ile Thr Gly Gly Gly
210 215 220
Ile Leu Met Pro Pro Asp Gly Tyr Met Gly Lys Val Lys Glu Ile Cys
225 230 235 240
Glu Lys His Gly Ala Leu Leu Ile Cys Asp Glu Val Ile Cys Gly Phe
245 250 255
Gly Arg Thr Gly Lys Pro Phe Gly Phe Met Asn Tyr Gly Val Lys Pro
260 265 270
Asp Ile Ile Thr Met Ala Lys Gly Ile Thr Ser Ala Tyr Leu Pro Leu
275 280 285
Ser Ala Thr Ala Val Arg Arg Glu Val Tyr Glu Ala Phe Val Gly Ser
290 295 300
Asp Asp Tyr Asp Arg Phe Arg His Val Asn Thr Phe Gly Gly Asn Pro
305 310 315 320
Ala Ala Cys Ala Leu Ala Leu Lys Asn Leu Glu Ile Met Glu Asn Glu
325 330 335
Lys Leu Ile Glu Arg Ser Lys Glu Leu Gly Glu Arg Leu Leu Tyr Glu
340 345 350
Leu Glu Asp Val Lys Glu His Pro Asn Val Gly Asp Val Arg Gly Lys
355 360 365
Gly Leu Leu Leu Gly Ile Glu Leu Val Glu Asp Lys Gln Thr Lys Glu
370 375 380
Pro Ala Ser Ile Glu Lys Met Asn Lys Val Ile Asn Ala Cys Lys Glu
385 390 395 400
Lys Gly Leu Ile Ile Gly Lys Asn Gly Asp Thr Val Ala Gly Tyr Asn
405 410 415
Asn Ile Leu Gln Leu Ala Pro Pro Leu Ser Ile Thr Glu Glu Asp Phe
420 425 430
Thr Phe Ile Val Lys Thr Met Lys Glu Cys Leu Ser Arg Ile Asn Gly
435 440 445
Gln
<210> 84
<211> 456
<212> PRT
<213> Pseudomonas aeruginosa
<400> 84
Met Asn Ser Gln Ile Thr Asn Ala Lys Thr Arg Glu Trp Gln Ala Leu
1 5 10 15
Ser Arg Asp His His Leu Pro Pro Phe Thr Asp Tyr Lys Gln Leu Asn
20 25 30
Glu Lys Gly Ala Arg Ile Ile Thr Lys Ala Glu Gly Val Tyr Ile Trp
35 40 45
Asp Ser Glu Gly Asn Lys Ile Leu Asp Ala Met Ala Gly Leu Trp Cys
50 55 60
Val Asn Val Gly Tyr Gly Arg Glu Glu Leu Val Gln Ala Ala Thr Arg
65 70 75 80
Gln Met Arg Glu Leu Pro Phe Tyr Asn Leu Phe Phe Gln Thr Ala His
85 90 95
Pro Pro Val Val Glu Leu Ala Lys Ala Ile Ala Asp Val Ala Pro Glu
100 105 110
Gly Met Asn His Val Phe Phe Thr Gly Ser Gly Ser Glu Ala Asn Asp
115 120 125
Thr Val Leu Arg Met Val Arg His Tyr Trp Ala Thr Lys Gly Gln Pro
130 135 140
Gln Lys Lys Val Val Ile Gly Arg Trp Asn Gly Tyr His Gly Ser Thr
145 150 155 160
Val Ala Gly Val Ser Leu Gly Gly Met Lys Ala Leu His Glu Gln Gly
165 170 175
Asp Phe Pro Ile Pro Gly Ile Val His Ile Ala Gln Pro Tyr Trp Tyr
180 185 190
Gly Glu Gly Gly Asp Met Ser Pro Asp Glu Phe Gly Val Trp Ala Ala
195 200 205
Glu Gln Leu Glu Lys Lys Ile Leu Glu Val Gly Glu Glu Asn Val Ala
210 215 220
Ala Phe Ile Ala Glu Pro Ile Gln Gly Ala Gly Gly Val Ile Val Pro
225 230 235 240
Pro Asp Thr Tyr Trp Pro Lys Ile Arg Glu Ile Leu Ala Lys Tyr Asp
245 250 255
Ile Leu Phe Ile Ala Asp Glu Val Ile Cys Gly Phe Gly Arg Thr Gly
260 265 270
Glu Trp Phe Gly Ser Gln Tyr Tyr Gly Asn Ala Pro Asp Leu Met Pro
275 280 285
Ile Ala Lys Gly Leu Thr Ser Gly Tyr Ile Pro Met Gly Gly Val Val
290 295 300
Val Arg Asp Glu Ile Val Glu Val Leu Asn Gln Gly Gly Glu Phe Tyr
305 310 315 320
His Gly Phe Thr Tyr Ser Gly His Pro Val Ala Ala Ala Val Ala Leu
325 330 335
Glu Asn Ile Arg Ile Leu Arg Glu Glu Lys Ile Ile Glu Lys Val Lys
340 345 350
Ala Glu Thr Ala Pro Tyr Leu Gln Lys Arg Trp Gln Glu Leu Ala Asp
355 360 365
His Pro Leu Val Gly Glu Ala Arg Gly Val Gly Met Val Ala Ala Leu
370 375 380
Glu Leu Val Lys Asn Lys Lys Thr Arg Glu Arg Phe Thr Asp Lys Gly
385 390 395 400
Val Gly Met Leu Cys Arg Glu His Cys Phe Arg Asn Gly Leu Ile Met
405 410 415
Arg Ala Val Gly Asp Thr Met Ile Ile Ser Pro Pro Leu Val Ile Asp
420 425 430
Pro Ser Gln Ile Asp Glu Leu Ile Thr Leu Ala Arg Lys Cys Leu Asp
435 440 445
Gln Thr Ala Ala Ala Val Leu Ala
450 455
<210> 85
<211> 538
<212> PRT
<213> Penicillium Chrysogenum
<400> 85
Met Thr Ala Leu Arg Ile Ser Thr Thr Leu Ser Arg Lys Ala Leu Thr
1 5 10 15
Ala Cys Arg Pro Arg Leu Phe Leu Gly Val Arg Thr Ser Val Arg Pro
20 25 30
Trp Gln Pro Phe Ser Thr Ser Thr Ser Thr Arg Tyr Ser Ile Thr Thr
35 40 45
Pro Leu Lys Ser Leu Ala Pro Lys Val Glu Arg Ser Gly Ser Lys Leu
50 55 60
Tyr Lys Asp Ala Asp Ala Ala Val Ala Asp Ile Lys Ser Gly Ser Thr
65 70 75 80
Ile Leu Ser Ser Gly Phe Gly Leu Cys Gly Val Ala Glu Thr Leu Ile
85 90 95
Asn Ala Met His Arg Arg Gly Ala Asp Gln Leu His Ser Leu Thr Ala
100 105 110
Val Ser Asn Asn Ala Gly Ala Ala Gly Lys Gly Gly Leu Ser Thr Leu
115 120 125
Ser Gln Asn Gly Gln Ile Asn Arg Leu Ile Leu Ser Tyr Leu Gly Asn
130 135 140
Asn Lys Ala Leu Glu Lys Lys Tyr Leu Thr Gly His Ile Ala Ile Glu
145 150 155 160
Leu Cys Pro Gln Gly Thr Leu Ala Glu Arg Leu Arg Ala Gly Gly Ala
165 170 175
Gly Ile Pro Ala Phe Phe Thr Pro Thr Gly Glu Gly Lys Ile Pro Val
180 185 190
Arg Met Asp Glu Ser Gly Lys Val Leu Glu Ser Gly Lys Pro Arg Glu
195 200 205
Thr Arg Glu Phe Asn Gly Lys Thr Tyr Leu Met Glu Glu Ala Leu Thr
210 215 220
Gly Asp Val Ala Ile Leu Arg Ala Trp Lys Ala Asp Glu Ala Gly Asn
225 230 235 240
Cys Val Phe Arg Tyr Thr Thr Lys Ala Phe Gly Pro Ile Met Ala Lys
245 250 255
Ala Ala Thr Leu Thr Ile Val Glu Ala Glu Asn Ile Val Pro Ile Gly
260 265 270
Ser Ile Asp Pro Asn Asp Val Asp Leu Pro Gly Ile Phe Val Asp Arg
275 280 285
Ile Val Pro Ala Thr Asp Asp Lys His Ile Glu Ile Arg Lys Leu Arg
290 295 300
Ser Gly Glu Thr Thr Val Ala Gly Ser Gly Lys Asp Glu Ala Arg Ile
305 310 315 320
Gln Arg Glu Leu Ile Gly Arg Arg Ala Ala Lys Glu Leu Lys Pro Gly
325 330 335
Phe Tyr Val Asn Leu Gly Val Gly Ile Pro Thr Leu Ala Pro Ser Phe
340 345 350
Leu Pro Lys Asp Val Lys Val Trp Ile Gln Ser Glu Asn Gly Ile Leu
355 360 365
Gly Met Gly Asp Tyr Pro Thr Glu Gln Glu Leu Asp Pro Asp Ile Ile
370 375 380
Asn Ala Gly Lys Glu Thr Val Thr Leu Val Pro Gly Ala Ala Thr Phe
385 390 395 400
Asp Ser Ser Glu Ser Phe Gly Met Ile Arg Gly Gly His Val Asp Val
405 410 415
Ser Ile Leu Gly Ala Leu Gln Val Ser Ala Asn Gly Asp Leu Ala Asn
420 425 430
Tyr Met Ile Pro Gly Lys Val Phe Lys Gly Met Gly Gly Ala Met Asp
435 440 445
Leu Ile Ser Asn Pro Glu Lys Thr Lys Ile Val Val Ala Thr Ser His
450 455 460
Val Ala Lys Asp Gly Ser Pro Lys Ile Val Gln Lys Cys Ser Leu Pro
465 470 475 480
Leu Thr Gly Ala Asn Val Val Ser Thr Ile Ile Thr Asp Leu Cys Val
485 490 495
Phe Gln Val Asp Arg Ala Thr Gly Glu Leu Thr Leu Thr Glu Leu Ala
500 505 510
Pro Gly Val Glu Val Glu Glu Val Gln Ser Lys Thr Asp Ala Lys Phe
515 520 525
Thr Ile Ala Asp Thr Leu Glu Ile Met Glu
530 535
<210> 86
<211> 1362
<212> DNA
<213> vibrio fluvialis
<220>
<221> CDS
<222> (1)..(1362)
<400> 86
atg aac aaa ccg caa agc tgg gaa gcc cgg gcc gag acc tat tcg ctc 48
Met Asn Lys Pro Gln Ser Trp Glu Ala Arg Ala Glu Thr Tyr Ser Leu
1 5 10 15
tat ggt ttc acc gac atg cct tcg ctg cat cag cgc ggc acg gtc gtc 96
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val
20 25 30
gtg acc cat ggc gag gga ccc tat atc gtc gat gtg aat ggc cgg cgt 144
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val Asn Gly Arg Arg
35 40 45
tat ctg gac gcc aac tcg ggc ctg tgg aac atg gtc gcg ggc ttt gac 192
Tyr Leu Asp Ala Asn Ser Gly Leu Trp Asn Met Val Ala Gly Phe Asp
50 55 60
cac aag ggg ctg atc gac gcc gcc aag gcc caa tac gag cgt ttt ccc 240
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro
65 70 75 80
ggt tat cac gcc ttt ttc ggc cgc atg tcc gat cag acg gta atg ctg 288
Gly Tyr His Ala Phe Phe Gly Arg Met Ser Asp Gln Thr Val Met Leu
85 90 95
tcg gaa aag ctg gtc gag gtg tcg ccc ttt gat tcg ggc cgg gtg ttc 336
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe
100 105 110
tat aca aac tcg ggg tcc gag gcg aat gac acc atg gtc aag atg cta 384
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu
115 120 125
tgg ttc ctg cat gca gcc gag ggc aaa ccg caa aag cgc aag atc ctg 432
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu
130 135 140
acc cgc tgg aac gcc tat cac ggc gtg acc gcc gtt tcg gcc agc atg 480
Thr Arg Trp Asn Ala Tyr His Gly Val Thr Ala Val Ser Ala Ser Met
145 150 155 160
acc ggc aag ccc tat aat tcg gtc ttt ggc ctg ccg ctg ccg ggc ttt 528
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe
165 170 175
gtg cat ctg acc tgc ccg cat tac tgg cgc tat ggc gaa gag ggc gaa 576
Val His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu
180 185 190
acc gaa gag cag ttc gtc gcc cgc ctc gcc cgc gag ctg gag gaa acg 624
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr
195 200 205
atc cag cgc gag ggc gcc gac acc atc gcc ggt ttc ttt gcc gaa ccg 672
Ile Gln Arg Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro
210 215 220
gtg atg ggc gcg ggc ggc gtg att ccc ccg gcc aag ggc tat ttc cag 720
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln
225 230 235 240
gcg atc ctg cca atc ctg cgc aaa tat gac atc ccg gtc atc tcg gac 768
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp
245 250 255
gag gtg atc tgc ggt ttc gga cgc acc ggt aac acc tgg ggc tgc gtg 816
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val
260 265 270
acc tat gac ttt aca ccc gat gca atc atc tcg tcc aag aat ctt aca 864
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr
275 280 285
gcg ggc ttt ttc ccc atg ggg gcg gtg atc ctt ggc ccg gaa ctt tcc 912
Ala Gly Phe Phe Pro Met Gly Ala Val Ile Leu Gly Pro Glu Leu Ser
290 295 300
aaa cgg ctg gaa acc gca atc gag gcg atc gag gaa ttc ccc cat ggc 960
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly
305 310 315 320
ttt acc gcc tcg ggc cat ccg gtc ggc tgt gct att gcg ctg aaa gca 1008
Phe Thr Ala Ser Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala
325 330 335
atc gac gtg gtg atg aat gaa ggg ctg gct gag aac gtc cgc cgc ctt 1056
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu
340 345 350
gcc ccc cgt ttc gag gaa agg ctg aaa cat atc gcc gag cgc ccg aac 1104
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn
355 360 365
atc ggt gaa tat cgc ggc atc ggc ttc atg tgg gcg ctg gag gct gtc 1152
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val
370 375 380
aag gac aag gca agc aag acg ccg ttc gac ggc aac ctg tcg gtc agc 1200
Lys Asp Lys Ala Ser Lys Thr Pro Phe Asp Gly Asn Leu Ser Val Ser
385 390 395 400
gag cgt atc gcc aat acc tgc acc gat ctg ggg ctg att tgc cgg ccg 1248
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro
405 410 415
ctt ggt cag tcc gtc gtc ctt tgt ccg ccc ttt atc ctg acc gag gcg 1296
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala
420 425 430
cag atg gat gag atg ttc gat aaa ctc gaa aaa gcc ctt gat aag gtc 1344
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val
435 440 445
ttt gcc gag gtt gcc tga 1362
Phe Ala Glu Val Ala
450
<210> 87
<211> 453
<212> PRT
<213> vibrio fluvialis
<400> 87
Met Asn Lys Pro Gln Ser Trp Glu Ala Arg Ala Glu Thr Tyr Ser Leu
1 5 10 15
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val
20 25 30
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val Asn Gly Arg Arg
35 40 45
Tyr Leu Asp Ala Asn Ser Gly Leu Trp Asn Met Val Ala Gly Phe Asp
50 55 60
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro
65 70 75 80
Gly Tyr His Ala Phe Phe Gly Arg Met Ser Asp Gln Thr Val Met Leu
85 90 95
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe
100 105 110
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu
115 120 125
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu
130 135 140
Thr Arg Trp Asn Ala Tyr His Gly Val Thr Ala Val Ser Ala Ser Met
145 150 155 160
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe
165 170 175
Val His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu
180 185 190
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr
195 200 205
Ile Gln Arg Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro
210 215 220
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln
225 230 235 240
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp
245 250 255
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val
260 265 270
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr
275 280 285
Ala Gly Phe Phe Pro Met Gly Ala Val Ile Leu Gly Pro Glu Leu Ser
290 295 300
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly
305 310 315 320
Phe Thr Ala Ser Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala
325 330 335
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu
340 345 350
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn
355 360 365
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val
370 375 380
Lys Asp Lys Ala Ser Lys Thr Pro Phe Asp Gly Asn Leu Ser Val Ser
385 390 395 400
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro
405 410 415
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala
420 425 430
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val
435 440 445
Phe Ala Glu Val Ala
450
<210> 88
<211> 1362
<212> DNA
<213> Artificial
<220>
<223> Vibrio fluvialis JS17 omega-aminotransferase codon optimised gene
<400> 88
atgaataaac cacagtcttg ggaagctcgt gctgaaacct atagcctgta cggctttacc 60
gatatgccgt ctctgcacca gcgtggtact gtagtggtaa cgcacggtga gggcccgtac 120
atcgtggacg ttaatggccg ccgttacctg gatgcaaaca gcggcctgtg gaacatggtt 180
gcgggcttcg accacaaagg cctgatcgat gccgcaaaag cgcagtacga acgcttcccg 240
ggttatcacg cgttctttgg ccgtatgagc gaccagactg tgatgctgag cgaaaaactg 300
gttgaagtgt ccccgttcga tagcggtcgt gtcttttaca ctaactctgg cagcgaggct 360
aacgatacca tggttaagat gctgtggttc ctgcacgcag cggaaggcaa acctcagaaa 420
cgtaaaattc tgacccgttg gaacgcttat cacggtgtga ctgctgtttc cgcatctatg 480
accggtaaac cgtataacag cgtgttcggt ctgccgctgc ctggcttcgt gcatctgacc 540
tgcccgcact actggcgtta tggtgaggaa ggcgaaactg aggaacagtt cgtggcgcgt 600
ctggctcgtg aactggaaga aaccattcaa cgcgaaggtg cagatactat cgcgggcttc 660
tttgcggagc ctgttatggg tgccggcggt gtgattccgc cggcgaaggg ctatttccag 720
gcaatcctgc cgatcctgcg caagtacgac attccggtta tttctgacga agtgatctgc 780
ggcttcggcc gcaccggtaa cacctggggc tgcgtgacgt atgacttcac tccggacgca 840
atcattagct ctaaaaacct gactgcgggt ttcttcccta tgggcgccgt aatcctgggc 900
ccagaactgt ctaagcgcct ggaaaccgcc atcgaggcaa tcgaagagtt cccgcacggt 960
ttcactgcta gcggccatcc ggtaggctgc gcaatcgcgc tgaaggcgat cgatgttgtc 1020
atgaacgagg gcctggcgga aaacgtgcgc cgcctggcgc cgcgttttga agaacgtctg 1080
aaacacattg ctgagcgccc gaacattggc gaatatcgcg gcatcggttt catgtgggcc 1140
ctggaagcag ttaaagataa agctagcaag accccgttcg acggcaacct gtccgtgagc 1200
gaacgtatcg ctaatacctg tacggacctg ggtctgatct gccgtccgct gggtcagtcc 1260
gtagttctgt gcccaccatt tatcctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggagaaag ctctggataa agtgttcgct gaagtcgcgt aa 1362
<210> 89
<211> 1350
<212> DNA
<213> Bacillus weihenstephanensis
<220>
<221> CDS
<222> (1)..(1350)
<400> 89
gtg caa gcg acg gag caa aca caa agt ttg aaa aaa aca gat gaa aag 48
Val Gln Ala Thr Glu Gln Thr Gln Ser Leu Lys Lys Thr Asp Glu Lys
1 5 10 15
tac ctt tgg cat gcg atg aga gga gca gcc cct agt cca acg aat tta 96
Tyr Leu Trp His Ala Met Arg Gly Ala Ala Pro Ser Pro Thr Asn Leu
20 25 30
att atc aca aaa gca gaa ggg gca tgg gtg acg gat att gat gga aac 144
Ile Ile Thr Lys Ala Glu Gly Ala Trp Val Thr Asp Ile Asp Gly Asn
35 40 45
cgt tat tta gac ggt atg tcc ggt ctt tgg tgc gtg aat gtt ggg tat 192
Arg Tyr Leu Asp Gly Met Ser Gly Leu Trp Cys Val Asn Val Gly Tyr
50 55 60
ggt cga aaa gaa ctt gca aga gcg gcg ttt gaa cag ctt gaa gaa atg 240
Gly Arg Lys Glu Leu Ala Arg Ala Ala Phe Glu Gln Leu Glu Glu Met
65 70 75 80
ccg tat ttc cct ctg act caa agt cat gtt cct gct att aaa tta gca 288
Pro Tyr Phe Pro Leu Thr Gln Ser His Val Pro Ala Ile Lys Leu Ala
85 90 95
gaa aaa ttg aat gaa tgg ctt gat gat gaa tac gtc att ttc ttt tct 336
Glu Lys Leu Asn Glu Trp Leu Asp Asp Glu Tyr Val Ile Phe Phe Ser
100 105 110
aac agt gga tcg gaa gcg aat gaa aca gca ttt aaa att gct cgt caa 384
Asn Ser Gly Ser Glu Ala Asn Glu Thr Ala Phe Lys Ile Ala Arg Gln
115 120 125
tat cat caa caa aaa ggt gat cat gga cgc tat aag ttt att tcc cgc 432
Tyr His Gln Gln Lys Gly Asp His Gly Arg Tyr Lys Phe Ile Ser Arg
130 135 140
tac cgc gct tat cac ggt aac tca atg gga gct ctt gca gca aca ggt 480
Tyr Arg Ala Tyr His Gly Asn Ser Met Gly Ala Leu Ala Ala Thr Gly
145 150 155 160
caa gca cag cga aag tat aaa tat gaa cca ctc ggg caa gga ttc ctg 528
Gln Ala Gln Arg Lys Tyr Lys Tyr Glu Pro Leu Gly Gln Gly Phe Leu
165 170 175
cat gta gca ccg cct gat acg tat cga aat cca gag gat gtt cat aca 576
His Val Ala Pro Pro Asp Thr Tyr Arg Asn Pro Glu Asp Val His Thr
180 185 190
ctg gca agt gct gag gaa atc gat cgt gtc atg aca tgg gag tta agc 624
Leu Ala Ser Ala Glu Glu Ile Asp Arg Val Met Thr Trp Glu Leu Ser
195 200 205
caa aca gta gcc ggt gtg att atg gag cca atc att act ggg ggc gga 672
Gln Thr Val Ala Gly Val Ile Met Glu Pro Ile Ile Thr Gly Gly Gly
210 215 220
att tta atg cct cct gat gga tat atg gga aaa gta aaa gaa att tgc 720
Ile Leu Met Pro Pro Asp Gly Tyr Met Gly Lys Val Lys Glu Ile Cys
225 230 235 240
gag aag cac ggt gcg ttg ctc att tgt gat gaa gtt ata tgt gga ttt 768
Glu Lys His Gly Ala Leu Leu Ile Cys Asp Glu Val Ile Cys Gly Phe
245 250 255
ggc cgg aca ggg aag cca ttt gga ttt atg aat tat ggc gtc aaa cca 816
Gly Arg Thr Gly Lys Pro Phe Gly Phe Met Asn Tyr Gly Val Lys Pro
260 265 270
gat atc att aca atg gca aaa ggt att aca agt gcg tat ctt cct ttg 864
Asp Ile Ile Thr Met Ala Lys Gly Ile Thr Ser Ala Tyr Leu Pro Leu
275 280 285
tca gca aca gca gtt aga cga gag gtt tat gag gca ttc gta ggt agt 912
Ser Ala Thr Ala Val Arg Arg Glu Val Tyr Glu Ala Phe Val Gly Ser
290 295 300
gat gat tat gat cgc ttc cgc cat gta aat acg ttc gga ggg aat cct 960
Asp Asp Tyr Asp Arg Phe Arg His Val Asn Thr Phe Gly Gly Asn Pro
305 310 315 320
gct gct tgc gct tta gct ttg aag aat tta gaa att atg gag aat gag 1008
Ala Ala Cys Ala Leu Ala Leu Lys Asn Leu Glu Ile Met Glu Asn Glu
325 330 335
aaa ctc att gaa cgt tcc aaa gaa ttg ggt gaa cga ctg tta tat gag 1056
Lys Leu Ile Glu Arg Ser Lys Glu Leu Gly Glu Arg Leu Leu Tyr Glu
340 345 350
cta gag gat gta aaa gag cat cca aac gta ggg gat gtt cgc gga aag 1104
Leu Glu Asp Val Lys Glu His Pro Asn Val Gly Asp Val Arg Gly Lys
355 360 365
ggc ctt ctt tta ggc att gaa cta gtg gaa gat aag caa aca aaa gaa 1152
Gly Leu Leu Leu Gly Ile Glu Leu Val Glu Asp Lys Gln Thr Lys Glu
370 375 380
ccg gct tcc att gaa aag atg aac aaa gtc atc aat gct tgt aaa gaa 1200
Pro Ala Ser Ile Glu Lys Met Asn Lys Val Ile Asn Ala Cys Lys Glu
385 390 395 400
aaa ggt cta att att ggt aaa aat ggt gac act gtc gca ggt tac aat 1248
Lys Gly Leu Ile Ile Gly Lys Asn Gly Asp Thr Val Ala Gly Tyr Asn
405 410 415
aat att ttg cag ctt gca cct cca tta agc atc aca gag gaa gac ttt 1296
Asn Ile Leu Gln Leu Ala Pro Pro Leu Ser Ile Thr Glu Glu Asp Phe
420 425 430
act ttt atc gtt aaa aca atg aaa gaa tgt tta tcc cgc att aac ggg 1344
Thr Phe Ile Val Lys Thr Met Lys Glu Cys Leu Ser Arg Ile Asn Gly
435 440 445
cag taa 1350
Gln
<210> 90
<211> 449
<212> PRT
<213> Bacillus weihenstephanensis
<400> 90
Val Gln Ala Thr Glu Gln Thr Gln Ser Leu Lys Lys Thr Asp Glu Lys
1 5 10 15
Tyr Leu Trp His Ala Met Arg Gly Ala Ala Pro Ser Pro Thr Asn Leu
20 25 30
Ile Ile Thr Lys Ala Glu Gly Ala Trp Val Thr Asp Ile Asp Gly Asn
35 40 45
Arg Tyr Leu Asp Gly Met Ser Gly Leu Trp Cys Val Asn Val Gly Tyr
50 55 60
Gly Arg Lys Glu Leu Ala Arg Ala Ala Phe Glu Gln Leu Glu Glu Met
65 70 75 80
Pro Tyr Phe Pro Leu Thr Gln Ser His Val Pro Ala Ile Lys Leu Ala
85 90 95
Glu Lys Leu Asn Glu Trp Leu Asp Asp Glu Tyr Val Ile Phe Phe Ser
100 105 110
Asn Ser Gly Ser Glu Ala Asn Glu Thr Ala Phe Lys Ile Ala Arg Gln
115 120 125
Tyr His Gln Gln Lys Gly Asp His Gly Arg Tyr Lys Phe Ile Ser Arg
130 135 140
Tyr Arg Ala Tyr His Gly Asn Ser Met Gly Ala Leu Ala Ala Thr Gly
145 150 155 160
Gln Ala Gln Arg Lys Tyr Lys Tyr Glu Pro Leu Gly Gln Gly Phe Leu
165 170 175
His Val Ala Pro Pro Asp Thr Tyr Arg Asn Pro Glu Asp Val His Thr
180 185 190
Leu Ala Ser Ala Glu Glu Ile Asp Arg Val Met Thr Trp Glu Leu Ser
195 200 205
Gln Thr Val Ala Gly Val Ile Met Glu Pro Ile Ile Thr Gly Gly Gly
210 215 220
Ile Leu Met Pro Pro Asp Gly Tyr Met Gly Lys Val Lys Glu Ile Cys
225 230 235 240
Glu Lys His Gly Ala Leu Leu Ile Cys Asp Glu Val Ile Cys Gly Phe
245 250 255
Gly Arg Thr Gly Lys Pro Phe Gly Phe Met Asn Tyr Gly Val Lys Pro
260 265 270
Asp Ile Ile Thr Met Ala Lys Gly Ile Thr Ser Ala Tyr Leu Pro Leu
275 280 285
Ser Ala Thr Ala Val Arg Arg Glu Val Tyr Glu Ala Phe Val Gly Ser
290 295 300
Asp Asp Tyr Asp Arg Phe Arg His Val Asn Thr Phe Gly Gly Asn Pro
305 310 315 320
Ala Ala Cys Ala Leu Ala Leu Lys Asn Leu Glu Ile Met Glu Asn Glu
325 330 335
Lys Leu Ile Glu Arg Ser Lys Glu Leu Gly Glu Arg Leu Leu Tyr Glu
340 345 350
Leu Glu Asp Val Lys Glu His Pro Asn Val Gly Asp Val Arg Gly Lys
355 360 365
Gly Leu Leu Leu Gly Ile Glu Leu Val Glu Asp Lys Gln Thr Lys Glu
370 375 380
Pro Ala Ser Ile Glu Lys Met Asn Lys Val Ile Asn Ala Cys Lys Glu
385 390 395 400
Lys Gly Leu Ile Ile Gly Lys Asn Gly Asp Thr Val Ala Gly Tyr Asn
405 410 415
Asn Ile Leu Gln Leu Ala Pro Pro Leu Ser Ile Thr Glu Glu Asp Phe
420 425 430
Thr Phe Ile Val Lys Thr Met Lys Glu Cys Leu Ser Arg Ile Asn Gly
435 440 445
Gln
<210> 91
<211> 1350
<212> DNA
<213> Artificial
<220>
<223> B. weihenstephanensis KBAB4 aminotransferase codon-optimised gene
<400> 91
atgcaggcta ccgaacaaac ccaatctctg aaaaagactg acgaaaaata tctgtggcac 60
gcgatgcgcg gtgcagctcc gtctccgacc aacctgatta ttaccaaagc tgaaggcgcg 120
tgggtgaccg acattgacgg taaccgttat ctggatggca tgagcggcct gtggtgtgtt 180
aatgtcggtt atggccgtaa ggagctggcg cgcgcggcat ttgaacaact ggaagaaatg 240
ccgtacttcc cgctgactca aagccatgtg ccggctatca aactggcgga aaaactgaac 300
gaatggctgg acgacgaata cgtgattttc ttctctaatt ctggctccga agcaaacgaa 360
accgcattca aaatcgcccg tcaatatcac cagcagaaag gtgaccacgg ccgctataaa 420
ttcatcagcc gttatcgtgc ataccatggt aattctatgg gtgcgctggc tgctaccggt 480
caggctcagc gcaaatacaa gtacgaaccg ctgggtcagg gttttctgca cgttgcacca 540
ccggatacct accgtaaccc ggaagacgtc cacaccctgg cttctgccga agaaatcgat 600
cgtgttatga cctgggagct gtcccagact gttgcgggtg ttatcatgga acctattatt 660
accggtggtg gcattctgat gccgccggac ggttatatgg gtaaagtcaa ggaaatctgc 720
gaaaaacacg gcgcgctgct gatctgcgat gaagttatct gtggcttcgg tcgcaccggc 780
aaaccatttg gcttcatgaa ttatggcgta aaacctgaca ttattaccat ggctaaaggc 840
attacttccg cttatctgcc gctgagcgcg accgcagttc gccgcgaagt ttatgaagcg 900
tttgttggtt ctgatgatta cgaccgtttc cgtcatgtaa acacgtttgg cggtaaccca 960
gcggcatgtg cgctggcgct gaaaaacctg gaaatcatgg aaaacgaaaa gctgatcgaa 1020
cgtagcaaag aactgggtga acgtctgctg tacgaactgg aagatgtcaa agaacacccg 1080
aacgtgggcg atgttcgcgg taaaggcctg ctgctgggta ttgaactggt tgaagacaaa 1140
cagaccaagg aaccggcttc cattgaaaag atgaacaaag tgattaacgc gtgcaaagag 1200
aaaggcctga tcattggtaa gaacggtgat accgtggcag gttataacaa cattctgcag 1260
ctggcgccgc ctctgagcat cactgaagaa gatttcacct tcatcgtcaa aactatgaag 1320
gagtgcctga gccgcatcaa tggtcagtaa 1350
<210> 92
<211> 255
<212> PRT
<213> Saccharomyces cerevisiae; Hydroxyacyl-thioester dehydratase type 2,mitochondrial (P38790)
<400> 92
Met Ala Glu Leu Arg Val Leu Val Ala Val Lys Arg Val Ile Asp Tyr
1 5 10 15
Ala Val Lys Ile Arg Val Lys Pro Asp Arg Thr Gly Val Val Thr Asp
20 25 30
Gly Val Lys His Ser Met Asn Pro Phe Cys Glu Ile Ala Val Glu Glu
35 40 45
Ala Val Arg Leu Lys Glu Lys Lys Leu Val Lys Glu Val Ile Ala Val
50 55 60
Ser Cys Gly Pro Ala Gln Cys Gln Glu Thr Ile Arg Thr Ala Leu Ala
65 70 75 80
Met Gly Ala Asp Arg Gly Ile His Val Glu Val Pro Pro Ala Glu Ala
85 90 95
Glu Arg Leu Gly Pro Leu Gln Val Ala Arg Val Leu Ala Lys Leu Ala
100 105 110
Glu Lys Glu Lys Val Asp Leu Val Leu Leu Gly Lys Gln Ala Ile Asp
115 120 125
Asp Asp Cys Asn Gln Thr Gly Gln Met Thr Ala Gly Phe Leu Asp Trp
130 135 140
Pro Gln Gly Thr Phe Ala Ser Gln Val Thr Leu Glu Gly Asp Lys Leu
145 150 155 160
Lys Val Glu Arg Glu Ile Asp Gly Gly Leu Glu Thr Leu Arg Leu Lys
165 170 175
Leu Pro Ala Val Val Thr Ala Asp Leu Arg Leu Asn Glu Pro Arg Tyr
180 185 190
Ala Thr Leu Pro Asn Ile Met Lys Ala Lys Lys Lys Lys Ile Glu Val
195 200 205
Ile Lys Pro Gly Asp Leu Gly Val Asp Leu Thr Ser Lys Leu Ser Val
210 215 220
Ile Ser Val Glu Asp Pro Pro Gln Arg Thr Ala Gly Val Lys Val Glu
225 230 235 240
Thr Thr Glu Asp Leu Val Ala Lys Leu Lys Glu Ile Gly Arg Ile
245 250 255
<210> 93
<211> 860
<212> DNA
<213> artificial
<220>
<223> Microscilla marina ATCC 23134 Peroxisomal trans-2-enoyl-CoA
reductase; EC=1.3.1.38 (A1ZFB1)
<400> 93
ggtaccggat ccctaacagg aggaattaac catggtccag aaagacctgt tcaaagacaa 60
agttgttctg gtaactggcg gtcgttctgg tatcggttac gcaatttctc agatgatgct 120
ggaactgggt gcgaaagttg ttatcgcttc tcgcaaagag gatctgctga aacaggctgc 180
tgaagaactg tctcagtacg gtgaatgctc ctacctggct tgcgacatcc gtgaatctga 240
ccagcgtact gcgctgatgg agaagatcaa agctgacaac ggtcgtctgg atatcctggt 300
taacaacgct ggcggtcagt tcccggcacc ggcagaaacc atctctgaaa acggctggga 360
cgctgttatc aacaacaacc tgaacggtac tttccacatg tcctctctga tggcgcgtca 420
cttcttcatc ccgcagaaag aaggttgcat catcaacatc atcgctaaca tctaccgcgg 480
tttcccgtct atggttcaca ccggtgcagc acgtgctggc gttgaaaacc tgaccaaaac 540
tctggcggtt gagtggggcg actacaacat ccgcgttaac gcgattgctc cgggtactat 600
cgaatcttcc ggtctggata cttacccgaa gccggtacag gacatcctgg gtgaagcgcg 660
tgctgcagta ccactgaaac gcttcggtac tgttactgaa atcgctaaca ccacttgctt 720
cctggcttct ccgctggctt cttacatctc cggtgtttct ctgtacgttg acggtgcaca 780
gcacctgaac ggtggtgacc cgatgaagct gactcgcctg atgcgtgcat tcaccaagtc 840
tcgtgagctc taataagctt 860
<210> 94
<211> 273
<212> PRT
<213> Microscilla marina ATCC 23134 Peroxisomal trans-2-enoyl-CoAreductase; EC=1.3.1.38 (A1ZFB1)
<400> 94
Met Val Gln Lys Asp Leu Phe Lys Asp Lys Val Val Leu Val Thr Gly
1 5 10 15
Gly Arg Ser Gly Ile Gly Tyr Ala Ile Ser Gln Met Met Leu Glu Leu
20 25 30
Gly Ala Lys Val Val Ile Ala Ser Arg Lys Glu Asp Leu Leu Lys Gln
35 40 45
Ala Ala Glu Glu Leu Ser Gln Tyr Gly Glu Cys Ser Tyr Leu Ala Cys
50 55 60
Asp Ile Arg Glu Ser Asp Gln Arg Thr Ala Leu Met Glu Lys Ile Lys
65 70 75 80
Ala Asp Asn Gly Arg Leu Asp Ile Leu Val Asn Asn Ala Gly Gly Gln
85 90 95
Phe Pro Ala Pro Ala Glu Thr Ile Ser Glu Asn Gly Trp Asp Ala Val
100 105 110
Ile Asn Asn Asn Leu Asn Gly Thr Phe His Met Ser Ser Leu Met Ala
115 120 125
Arg His Phe Phe Ile Pro Gln Lys Glu Gly Cys Ile Ile Asn Ile Ile
130 135 140
Ala Asn Ile Tyr Arg Gly Phe Pro Ser Met Val His Thr Gly Ala Ala
145 150 155 160
Arg Ala Gly Val Glu Asn Leu Thr Lys Thr Leu Ala Val Glu Trp Gly
165 170 175
Asp Tyr Asn Ile Arg Val Asn Ala Ile Ala Pro Gly Thr Ile Glu Ser
180 185 190
Ser Gly Leu Asp Thr Tyr Pro Lys Pro Val Gln Asp Ile Leu Gly Glu
195 200 205
Ala Arg Ala Ala Val Pro Leu Lys Arg Phe Gly Thr Val Thr Glu Ile
210 215 220
Ala Asn Thr Thr Cys Phe Leu Ala Ser Pro Leu Ala Ser Tyr Ile Ser
225 230 235 240
Gly Val Ser Leu Tyr Val Asp Gly Ala Gln His Leu Asn Gly Gly Asp
245 250 255
Pro Met Lys Leu Thr Arg Leu Met Arg Ala Phe Thr Lys Ser Arg Glu
260 265 270
Thr
<210> 95
<211> 1241
<212> DNA
<213> Artificial
<220>
<223> Clostridium beijerinckii (strain ATCC 51743 / NCIMB 8052),
Putative reductase Cbei_2086; EC=1.3.1 (A6LV73)
<400> 95
ggtaccggat ccctaacagg aggaattaat catgatcttc aagccggaac tgatcaaagg 60
tatcgctaaa acttctcacc cgtacggttg ccgtaaagaa gttctgaacc agatcgaata 120
ctgcaaaaac gctaagcagt tccacggtcc gaagaaagtt ctgatcatcg gtgcttcttc 180
cggtttcggt ctggcaactc gcatttctct ggcgttcggt ggtgcgaaag cagacaccat 240
cggtgtttct ttcgaaaccg gtatcactga ccgccgtact ggtactgcag gctggtacaa 300
caacatcttc ttcaaagagt tcgcagaaaa agaaggtctg attgctaaaa acttcatcgg 360
tgacgcattc tctgacgaag ttaaagaaaa cgttatcaaa tacatcaaaa acgagttcgg 420
taagatcgat ctgctgatct actctctggc ttctccgcgc cgtaaagatc cgaaaaccgg 480
taacatctac gactccactc tgaaaaccac ttctggcgag ttccagggcc caaccatcga 540
catggaaact gacgaactgg ttactaccaa agttaactcc gcaactgaca aagaaatcga 600
agcgaccaag aaagtaatgg gtggtgaaga ttggtctgaa tggtgcaaac tgctgctgga 660
aaacgactgc ctgtctgaca aagctatcac tatctcttac tcttacatcg gtgcttctcg 720
cacttacaaa atctaccgtg aaggtactat cggtgaagcg aagcgtcacc tggaaaacac 780
tgcgattcag atcgacaaag aatggcagaa gaagatcaac ggtaaagcat tcgtttctgt 840
taacaaagca atcgttacca aggcttctgc ttacatcccg tctttctctc tgtacgctgc 900
tgttctgtac aaagtaatga aagaaaaaaa tctgcacgaa aactgcatca tgcagatgca 960
gcgtatgttc gctgacaaaa tctacgctga aaatctgctg gagttcgacg actccggtcg 1020
tctgcgtatg gacgactggg aactgcgtga agatgtacag tctgaagtta acgaactgtg 1080
ggaaaaaatc actccggata acttcaagat cctgtctgac tacgacggtt acaagaaaga 1140
gttcatgcag ctgaacggct tcgaaatcga cggtgttaac tactctgaag atatcgacat 1200
cgaagcgctg aagcgcctgg aaccggagct ctaataagct t 1241
<210> 96
<211> 400
<212> PRT
<213> Clostridium beijerinckii (strain ATCC 51743 / NCIMB 8052), Putativereductase Cbei_2086; EC=1.3.1 (A6LV73)
<400> 96
Met Ile Phe Lys Pro Glu Leu Ile Lys Gly Ile Ala Lys Thr Ser His
1 5 10 15
Pro Tyr Gly Cys Arg Lys Glu Val Leu Asn Gln Ile Glu Tyr Cys Lys
20 25 30
Asn Ala Lys Gln Phe His Gly Pro Lys Lys Val Leu Ile Ile Gly Ala
35 40 45
Ser Ser Gly Phe Gly Leu Ala Thr Arg Ile Ser Leu Ala Phe Gly Gly
50 55 60
Ala Lys Ala Asp Thr Ile Gly Val Ser Phe Glu Thr Gly Ile Thr Asp
65 70 75 80
Arg Arg Thr Gly Thr Ala Gly Trp Tyr Asn Asn Ile Phe Phe Lys Glu
85 90 95
Phe Ala Glu Lys Glu Gly Leu Ile Ala Lys Asn Phe Ile Gly Asp Ala
100 105 110
Phe Ser Asp Glu Val Lys Glu Asn Val Ile Lys Tyr Ile Lys Asn Glu
115 120 125
Phe Gly Lys Ile Asp Leu Leu Ile Tyr Ser Leu Ala Ser Pro Arg Arg
130 135 140
Lys Asp Pro Lys Thr Gly Asn Ile Tyr Asp Ser Thr Leu Lys Thr Thr
145 150 155 160
Ser Gly Glu Phe Gln Gly Pro Thr Ile Asp Met Glu Thr Asp Glu Leu
165 170 175
Val Thr Thr Lys Val Asn Ser Ala Thr Asp Lys Glu Ile Glu Ala Thr
180 185 190
Lys Lys Val Met Gly Gly Glu Asp Trp Ser Glu Trp Cys Lys Leu Leu
195 200 205
Leu Glu Asn Asp Cys Leu Ser Asp Lys Ala Ile Thr Ile Ser Tyr Ser
210 215 220
Tyr Ile Gly Ala Ser Arg Thr Tyr Lys Ile Tyr Arg Glu Gly Thr Ile
225 230 235 240
Gly Glu Ala Lys Arg His Leu Glu Asn Thr Ala Ile Gln Ile Asp Lys
245 250 255
Glu Trp Gln Lys Lys Ile Asn Gly Lys Ala Phe Val Ser Val Asn Lys
260 265 270
Ala Ile Val Thr Lys Ala Ser Ala Tyr Ile Pro Ser Phe Ser Leu Tyr
275 280 285
Ala Ala Val Leu Tyr Lys Val Met Lys Glu Lys Asn Leu His Glu Asn
290 295 300
Cys Ile Met Gln Met Gln Arg Met Phe Ala Asp Lys Ile Tyr Ala Glu
305 310 315 320
Asn Leu Leu Glu Phe Asp Asp Ser Gly Arg Leu Arg Met Asp Asp Trp
325 330 335
Glu Leu Arg Glu Asp Val Gln Ser Glu Val Asn Glu Leu Trp Glu Lys
340 345 350
Ile Thr Pro Asp Asn Phe Lys Ile Leu Ser Asp Tyr Asp Gly Tyr Lys
355 360 365
Lys Glu Phe Met Gln Leu Asn Gly Phe Glu Ile Asp Gly Val Asn Tyr
370 375 380
Ser Glu Asp Ile Asp Ile Glu Ala Leu Lys Arg Leu Glu Pro Glu Thr
385 390 395 400
<210> 97
<211> 1238
<212> DNA
<213> Artificial
<220>
<223> Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB
9240), Trans-2-enoyl-CoA reductase; EC=1.3.1.44 (A0KLI7)
<400> 97
ggtaccggat ccctaacagg aggaattaat catgatcatc aagccgaaag ttcgtggctt 60
catctgcacc actactcacc cggttggttg cgaagctaac gttcgtcgtc agatcgctta 120
caccaaagcg aaaggtacta tcgaaaacgg tccgaagaaa gttctggtta tcggtgcttc 180
taccggttac ggtctggctt ctcgcattgc tgctgcattc ggttctggtg cggcaactct 240
gggcgtattc ttcgaaaaag cgggttctga aaccaaaact gcaactgctg gctggtacaa 300
ctccgcagca ttcgacaaag cagctaaaga agcgggtctg tacgctaaat ctatcaacgg 360
tgacgcattc tctaacgaat gccgcgctaa agttatcgaa ctgatcaagc aggacctggg 420
tcagatcgac ctggttgttt actctctggc ttctccggta cgtaagctgc cggataccgg 480
tgaagttgtt cgttctgcac tgaaaccgat cggtgaagtt tacaccacta ctgctatcga 540
caccaacaaa gaccagatca tcactgcaac cgttgagccg gctaacgaag aagaaatcca 600
gaacaccatc accgtaatgg gtggtcagga ctgggaactg tggatggctg cgctgcgcga 660
tgctggcgta ctggcagacg gtgcgaagtc tgttgcttac tcttacatcg gtactgacct 720
gacctggcca atctactggc acggtactct gggtcgtgcg aaagaagatt tggaccgcgc 780
tgcagctgct atccgcggtg acctggcagg caaaggtggt actgcgcacg ttgctgtact 840
gaaatctgtt gttactcagg cttcttctgc gattccggta atgccgctgt acatctctat 900
ggcattcaag attatgaaag agaaaggtat ccacgaaggt tgcatggaac aggttgaccg 960
tatgatgcgt actcgtctgt acgctgcaga catggcgctg gatgaccagg cgcgtatccg 1020
tatggacgac tgggaactgc gtgaagatgt tcagcagact tgccgcgacc tgtggccgtc 1080
tatcacttct gaaaacctgt gcgaactgac tgactacacc ggttacaagc aggagttcct 1140
gcgtctgttc ggtttcggtc tggaagaagt tgactacgac gctgacgtta acccggacgt 1200
taagttcgac gttgttgaac tagagctcta ataagctt 1238
<210> 98
<211> 399
<212> PRT
<213> Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB9240), Trans-2-enoyl-CoA reductase; EC=1.3.1.44 (A0KLI7)
<400> 98
Met Ile Ile Lys Pro Lys Val Arg Gly Phe Ile Cys Thr Thr Thr His
1 5 10 15
Pro Val Gly Cys Glu Ala Asn Val Arg Arg Gln Ile Ala Tyr Thr Lys
20 25 30
Ala Lys Gly Thr Ile Glu Asn Gly Pro Lys Lys Val Leu Val Ile Gly
35 40 45
Ala Ser Thr Gly Tyr Gly Leu Ala Ser Arg Ile Ala Ala Ala Phe Gly
50 55 60
Ser Gly Ala Ala Thr Leu Gly Val Phe Phe Glu Lys Ala Gly Ser Glu
65 70 75 80
Thr Lys Thr Ala Thr Ala Gly Trp Tyr Asn Ser Ala Ala Phe Asp Lys
85 90 95
Ala Ala Lys Glu Ala Gly Leu Tyr Ala Lys Ser Ile Asn Gly Asp Ala
100 105 110
Phe Ser Asn Glu Cys Arg Ala Lys Val Ile Glu Leu Ile Lys Gln Asp
115 120 125
Leu Gly Gln Ile Asp Leu Val Val Tyr Ser Leu Ala Ser Pro Val Arg
130 135 140
Lys Leu Pro Asp Thr Gly Glu Val Val Arg Ser Ala Leu Lys Pro Ile
145 150 155 160
Gly Glu Val Tyr Thr Thr Thr Ala Ile Asp Thr Asn Lys Asp Gln Ile
165 170 175
Ile Thr Ala Thr Val Glu Pro Ala Asn Glu Glu Glu Ile Gln Asn Thr
180 185 190
Ile Thr Val Met Gly Gly Gln Asp Trp Glu Leu Trp Met Ala Ala Leu
195 200 205
Arg Asp Ala Gly Val Leu Ala Asp Gly Ala Lys Ser Val Ala Tyr Ser
210 215 220
Tyr Ile Gly Thr Asp Leu Thr Trp Pro Ile Tyr Trp His Gly Thr Leu
225 230 235 240
Gly Arg Ala Lys Glu Asp Leu Asp Arg Ala Ala Ala Ala Ile Arg Gly
245 250 255
Asp Leu Ala Gly Lys Gly Gly Thr Ala His Val Ala Val Leu Lys Ser
260 265 270
Val Val Thr Gln Ala Ser Ser Ala Ile Pro Val Met Pro Leu Tyr Ile
275 280 285
Ser Met Ala Phe Lys Ile Met Lys Glu Lys Gly Ile His Glu Gly Cys
290 295 300
Met Glu Gln Val Asp Arg Met Met Arg Thr Arg Leu Tyr Ala Ala Asp
305 310 315 320
Met Ala Leu Asp Asp Gln Ala Arg Ile Arg Met Asp Asp Trp Glu Leu
325 330 335
Arg Glu Asp Val Gln Gln Thr Cys Arg Asp Leu Trp Pro Ser Ile Thr
340 345 350
Ser Glu Asn Leu Cys Glu Leu Thr Asp Tyr Thr Gly Tyr Lys Gln Glu
355 360 365
Phe Leu Arg Leu Phe Gly Phe Gly Leu Glu Glu Val Asp Tyr Asp Ala
370 375 380
Asp Val Asn Pro Asp Val Lys Phe Asp Val Val Glu Leu Glu Thr
385 390 395
<210> 99
<211> 1142
<212> DNA
<213> Artificial
<220>
<223> Yarrowia lipolytica, Probable trans-2-enoyl-CoA reductase,
mitochondrial; EC=1.3.1.38, (Q6CBE4)
<400> 99
ggtaccggat ccctaacagg aggaattaac catggccggc ttcggtactc cgtctttcgg 60
tctgcgcttc aactccgttg gtcgtgcatt cgtattctct cagactggcg aaccgaaaga 120
cgttattcag gtactggaat acccaatcga aaaaccgctg gaaaaccagg ttctgctgaa 180
atctctgggc ttcactatca acccggctga catcaaccag ctggaaggtg tttacccgtc 240
tgtaccgccg aaatctgtac agatcaacaa cgaagatgct gctatcggtg gtaacgaagg 300
tctgttccag gttctggacc cgggtgctaa atctggtctg aagaaaggcg actgggtact 360
gccgcgtaaa acttgcttcg gcacctggcg ttctcacgcg ctggttgaag ctgataccgt 420
tgttaagatc gacaacaccg acctgaccaa agttcaggca actaccgttt ctgttaaccc 480
gtctactgct tacgaaatgc tgaaagacct gaaagaaggt gactggttca tccagaacgg 540
tggtaactcc ggtgttggtc gtgctgcgat tcagatcggt cacatccgcg gtctgaaatc 600
tatctccgtt gttcgtgacc gtccggatct ggaagttctg aagaaagaac tgactgacct 660
gggtgcaact cacgttatca ctgaagaaga agcgtctgac aaactgttct ccaagcagat 720
caaatcctgg actggcggta aaatcaagct ggcactgaac tgcatcggtg gtaaatctgc 780
aacttccatc atgcgtcagc tgggtgctgg cggttctatc gttacttacg gtggtatgtc 840
caagaagccg ctgactttcc cgactggtcc gttcatcttc aaagacatca ctgcgaaagg 900
ttactggctg actcgctggg ctgacaagca cccggaagaa aaagcgaaaa ctatcgaaaa 960
catcttcaaa ttctaccgcg agaagaaatt cgttgctccg ccagttaaca tctccactct 1020
ggacttctct aaaggtaacg acgttgttct gtctgagttc ctggatgcac tgggtaaagc 1080
acagaaaggt ggtggtaaga agcagctggt acagtgggtt gaatacgagc tctaataagc 1140
tt 1142
<210> 100
<211> 367
<212> PRT
<213> Yarrowia lipolytica, Probable trans-2-enoyl-CoA reductase,mitochondrial; EC=1.3.1.38, (Q6CBE4)
<400> 100
Met Ala Gly Phe Gly Thr Pro Ser Phe Gly Leu Arg Phe Asn Ser Val
1 5 10 15
Gly Arg Ala Phe Val Phe Ser Gln Thr Gly Glu Pro Lys Asp Val Ile
20 25 30
Gln Val Leu Glu Tyr Pro Ile Glu Lys Pro Leu Glu Asn Gln Val Leu
35 40 45
Leu Lys Ser Leu Gly Phe Thr Ile Asn Pro Ala Asp Ile Asn Gln Leu
50 55 60
Glu Gly Val Tyr Pro Ser Val Pro Pro Lys Ser Val Gln Ile Asn Asn
65 70 75 80
Glu Asp Ala Ala Ile Gly Gly Asn Glu Gly Leu Phe Gln Val Leu Asp
85 90 95
Pro Gly Ala Lys Ser Gly Leu Lys Lys Gly Asp Trp Val Leu Pro Arg
100 105 110
Lys Thr Cys Phe Gly Thr Trp Arg Ser His Ala Leu Val Glu Ala Asp
115 120 125
Thr Val Val Lys Ile Asp Asn Thr Asp Leu Thr Lys Val Gln Ala Thr
130 135 140
Thr Val Ser Val Asn Pro Ser Thr Ala Tyr Glu Met Leu Lys Asp Leu
145 150 155 160
Lys Glu Gly Asp Trp Phe Ile Gln Asn Gly Gly Asn Ser Gly Val Gly
165 170 175
Arg Ala Ala Ile Gln Ile Gly His Ile Arg Gly Leu Lys Ser Ile Ser
180 185 190
Val Val Arg Asp Arg Pro Asp Leu Glu Val Leu Lys Lys Glu Leu Thr
195 200 205
Asp Leu Gly Ala Thr His Val Ile Thr Glu Glu Glu Ala Ser Asp Lys
210 215 220
Leu Phe Ser Lys Gln Ile Lys Ser Trp Thr Gly Gly Lys Ile Lys Leu
225 230 235 240
Ala Leu Asn Cys Ile Gly Gly Lys Ser Ala Thr Ser Ile Met Arg Gln
245 250 255
Leu Gly Ala Gly Gly Ser Ile Val Thr Tyr Gly Gly Met Ser Lys Lys
260 265 270
Pro Leu Thr Phe Pro Thr Gly Pro Phe Ile Phe Lys Asp Ile Thr Ala
275 280 285
Lys Gly Tyr Trp Leu Thr Arg Trp Ala Asp Lys His Pro Glu Glu Lys
290 295 300
Ala Lys Thr Ile Glu Asn Ile Phe Lys Phe Tyr Arg Glu Lys Lys Phe
305 310 315 320
Val Ala Pro Pro Val Asn Ile Ser Thr Leu Asp Phe Ser Lys Gly Asn
325 330 335
Asp Val Val Leu Ser Glu Phe Leu Asp Ala Leu Gly Lys Ala Gln Lys
340 345 350
Gly Gly Gly Lys Lys Gln Leu Val Gln Trp Val Glu Tyr Glu Thr
355 360 365
<210> 101
<211> 1856
<212> DNA
<213> Artificial
<220>
<223> Clostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680)
(A5N5C9)
<400> 101
ggtaccggat ccctaacagg aggaattaac catggacctg gcagaataca aaggcgtatg 60
ggtattcgct gaacagcgtg acggtgaact gcagaaagtt gcgctgcagc tggttggtaa 120
aggtcgtgaa ctggctgaca ctctgggtgt tgaactgact gcggtactgc tgggttctga 180
agttgatgac ctggcgaaag aactggttgc ttacggcgca gacaacgtac tgtacgcaga 240
ctctccgctg ctgaagcact acaccactga cggttacacc aaagttatcg acgaactgat 300
caaagagcgt aagccggaaa tcctgctgat cggtgcgact ttcatcggtc gtgacctggg 360
tccgcgcgtt gctggtcgcg tattcaccgg tctgactgca gactgcaccg gtctggatat 420
cgacgaagcg accaagaacc tgatgatgac tcgtccggca ttcggtggta acctgatggc 480
aactatcgct tgcgaaaaaa ctcgtccgca gatgtccacc gttcgtccgg gcgtattcaa 540
cgcactgccg cgcgacgctt cccgtactgg taagatcgaa aaaatcgctg ctaacgttgc 600
taaagacgac atccgtatcg aagttctgga agttgttaag tctgctggcg acaccatcga 660
catctctgaa gcagacgtta tcgtttctgg tggtcgcggt ctgggtggtc cggacggctt 720
caaagttctg aaagaactgg ctgatctgct gggtggtact atcggtggtt ctcgcgctac 780
catcgacgct ggctggatcg acaagtctta ccaggttggt cagactggta aaaccgttcg 840
tccgggtctg tacatcgctt gcggtatctc cggtcagatt cagcacctgg caggcatgca 900
ggattccggc ttcatcgttg ctatcaacaa agacgaaaac gcaccgatga tgcaagttgc 960
tgatctggcg attgttggtg acctgtacaa agttgttcca gagttcgttg aacaggttaa 1020
agcgctgaac ttgtaataac tagtctaaca ggaggaatta atcatgaaga tcgttgtttg 1080
cctgaaacag gttccggata ccactgaagt taagatcgac ccgaaaaccg gtactctgat 1140
ccgtgaaggc gtaccgtcta tcatcaaccc ggatgacaaa aacgcgctgg aagaatccat 1200
tgctctgaaa gagaaagttg gcggcaccgt aactgttgtt tctatgggtc cgccacaggc 1260
tgttgatgcg ctgcgtgaag cgctggcaat gggtgctgac gaagcaatcc tggtatctga 1320
ccgcgctttc gctggtgctg acactcaggc aacttcttac gcactggcag gcgcactgaa 1380
aaacctggaa tacgacctga ttttcgctgg tcgtcaggct atcgacggtg acactgcaca 1440
ggttggtccg cagatcgctg aaaaactggg tatcccgcag atcacttacg ttgaaaaagt 1500
tgacgttgac ggtgacactc tgaccgtaca gcgtgcatgg gaagatggtt acgaagttgc 1560
gaaaatcaaa actccgtgca tgctgactgc tatcaaagag ctgaaccagc cgcgctacat 1620
gaacatgaag aacatcttcg aagtattcaa gaaagaagtt aaaatctggt ctgctgacga 1680
cctggacgtt gacaaaaaca agctgggtct gaacggttcc tgcaccaaag ttaagcgttc 1740
tcacaccaaa gaagcgaaag gtgctggcga aatcgttaac aagccgatca aagaagcggt 1800
tgcttactcc atctccaagc tgcgtgaaaa acacgttatc gagctctaat aagctt 1856
<210> 102
<211> 334
<212> PRT
<213> Clostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680)(A5N5C9)
<400> 102
Met Asp Leu Ala Glu Tyr Lys Gly Val Trp Val Phe Ala Glu Gln Arg
1 5 10 15
Asp Gly Glu Leu Gln Lys Val Ala Leu Gln Leu Val Gly Lys Gly Arg
20 25 30
Glu Leu Ala Asp Thr Leu Gly Val Glu Leu Thr Ala Val Leu Leu Gly
35 40 45
Ser Glu Val Asp Asp Leu Ala Lys Glu Leu Val Ala Tyr Gly Ala Asp
50 55 60
Asn Val Leu Tyr Ala Asp Ser Pro Leu Leu Lys His Tyr Thr Thr Asp
65 70 75 80
Gly Tyr Thr Lys Val Ile Asp Glu Leu Ile Lys Glu Arg Lys Pro Glu
85 90 95
Ile Leu Leu Ile Gly Ala Thr Phe Ile Gly Arg Asp Leu Gly Pro Arg
100 105 110
Val Ala Gly Arg Val Phe Thr Gly Leu Thr Ala Asp Cys Thr Gly Leu
115 120 125
Asp Ile Asp Glu Ala Thr Lys Asn Leu Met Met Thr Arg Pro Ala Phe
130 135 140
Gly Gly Asn Leu Met Ala Thr Ile Ala Cys Glu Lys Thr Arg Pro Gln
145 150 155 160
Met Ser Thr Val Arg Pro Gly Val Phe Asn Ala Leu Pro Arg Asp Ala
165 170 175
Ser Arg Thr Gly Lys Ile Glu Lys Ile Ala Ala Asn Val Ala Lys Asp
180 185 190
Asp Ile Arg Ile Glu Val Leu Glu Val Val Lys Ser Ala Gly Asp Thr
195 200 205
Ile Asp Ile Ser Glu Ala Asp Val Ile Val Ser Gly Gly Arg Gly Leu
210 215 220
Gly Gly Pro Asp Gly Phe Lys Val Leu Lys Glu Leu Ala Asp Leu Leu
225 230 235 240
Gly Gly Thr Ile Gly Gly Ser Arg Ala Thr Ile Asp Ala Gly Trp Ile
245 250 255
Asp Lys Ser Tyr Gln Val Gly Gln Thr Gly Lys Thr Val Arg Pro Gly
260 265 270
Leu Tyr Ile Ala Cys Gly Ile Ser Gly Gln Ile Gln His Leu Ala Gly
275 280 285
Met Gln Asp Ser Gly Phe Ile Val Ala Ile Asn Lys Asp Glu Asn Ala
290 295 300
Pro Met Met Gln Val Ala Asp Leu Ala Ile Val Gly Asp Leu Tyr Lys
305 310 315 320
Val Val Pro Glu Phe Val Glu Gln Val Lys Ala Leu Asn Leu
325 330
<210> 103
<211> 259
<212> PRT
<213> Clostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680)(A5N5D0)
<400> 103
Met Lys Ile Val Val Cys Leu Lys Gln Val Pro Asp Thr Thr Glu Val
1 5 10 15
Lys Ile Asp Pro Lys Thr Gly Thr Leu Ile Arg Glu Gly Val Pro Ser
20 25 30
Ile Ile Asn Pro Asp Asp Lys Asn Ala Leu Glu Glu Ser Ile Ala Leu
35 40 45
Lys Glu Lys Val Gly Gly Thr Val Thr Val Val Ser Met Gly Pro Pro
50 55 60
Gln Ala Val Asp Ala Leu Arg Glu Ala Leu Ala Met Gly Ala Asp Glu
65 70 75 80
Ala Ile Leu Val Ser Asp Arg Ala Phe Ala Gly Ala Asp Thr Gln Ala
85 90 95
Thr Ser Tyr Ala Leu Ala Gly Ala Leu Lys Asn Leu Glu Tyr Asp Leu
100 105 110
Ile Phe Ala Gly Arg Gln Ala Ile Asp Gly Asp Thr Ala Gln Val Gly
115 120 125
Pro Gln Ile Ala Glu Lys Leu Gly Ile Pro Gln Ile Thr Tyr Val Glu
130 135 140
Lys Val Asp Val Asp Gly Asp Thr Leu Thr Val Gln Arg Ala Trp Glu
145 150 155 160
Asp Gly Tyr Glu Val Ala Lys Ile Lys Thr Pro Cys Met Leu Thr Ala
165 170 175
Ile Lys Glu Leu Asn Gln Pro Arg Tyr Met Asn Met Lys Asn Ile Phe
180 185 190
Glu Val Phe Lys Lys Glu Val Lys Ile Trp Ser Ala Asp Asp Leu Asp
195 200 205
Val Asp Lys Asn Lys Leu Gly Leu Asn Gly Ser Cys Thr Lys Val Lys
210 215 220
Arg Ser His Thr Lys Glu Ala Lys Gly Ala Gly Glu Ile Val Asn Lys
225 230 235 240
Pro Ile Lys Glu Ala Val Ala Tyr Ser Ile Ser Lys Leu Arg Glu Lys
245 250 255
His Val Ile
<210> 104
<211> 1196
<212> DNA
<213> Artificial
<220>
<223> Megasphaera elsdenii, Acyl-CoA dehydrogenase, short-chain
specific; EC=1.3.99.2 (Q06319)
<400> 104
ggtaccggat ccctaacagg aggaattaac catggacttc aacctgactg atatccagca 60
ggacttcctg aaactggcac acgacttcgg tgaaaaaaaa ctggcaccaa ccgtaactga 120
acgtgaccac aaaggtatct acgacaaaga gctgatcgac gaactgctgt ctctgggtat 180
cactggcgct tacttcgaag agaaatacgg tggttctggt gacgacggtg gtgacgttct 240
gtcttacatc ctggctgttg aagaactggc gaaatacgac gctggcgttg ctatcactct 300
gtctgcaacc gtttctctgt gcgcaaaccc gatctggcag ttcggtactg aagcgcagaa 360
agagaagttc ctggttccgc tggttgaagg tactaaactg ggtgcattcg gtctgactga 420
accgaacgct ggtactgacg cttctggtca gcagactatc gctaccaaaa acgacgacgg 480
tacttacact ctgaacggtt ctaaaatctt catcactaac ggtggtgcag ctgacatcta 540
catcgttttc gcaatgactg acaagtctaa aggtaaccac ggtatcactg cattcatcct 600
ggaagatggt actccgggct tcacttacgg taagaaagaa gataaaatgg gtatccacac 660
ttctcagact atggaactgg tattccagga cgttaaagtt ccggcagaaa acatgctggg 720
tgaagaaggt aaaggcttca aaatcgcgat gatgactctg gacggtggtc gtatcggtgt 780
tgctgctcag gcgctgggta tcgctgaagc ggcactggct gacgcggtag aatactccaa 840
gcagcgtgta cagttcggta agccgctgtg caaattccag tctatctcct tcaaactggc 900
tgatatgaag atgcagatcg aagcagctcg caacctggtt tacaaagcgg catgtaagaa 960
gcaggaaggt aagccgttca ccgttgatgc tgctatcgct aaacgcgttg cttctgacgt 1020
tgcgatgcgc gtaactactg aagctgttca gattttcggt ggttacggtt actctgaaga 1080
atacccggtt gcgcgtcaca tgcgtgacgc taaaatcact cagatttacg aaggtactaa 1140
cgaagttcag ctgatggtta ctggcggtgc gctgctgcgt gagctctaat aagctt 1196
<210> 105
<211> 385
<212> PRT
<213> Megasphaera elsdenii, Acyl-CoA dehydrogenase, short-chain specific;EC=1.3.99.2 (Q06319)
<400> 105
Met Asp Phe Asn Leu Thr Asp Ile Gln Gln Asp Phe Leu Lys Leu Ala
1 5 10 15
His Asp Phe Gly Glu Lys Lys Leu Ala Pro Thr Val Thr Glu Arg Asp
20 25 30
His Lys Gly Ile Tyr Asp Lys Glu Leu Ile Asp Glu Leu Leu Ser Leu
35 40 45
Gly Ile Thr Gly Ala Tyr Phe Glu Glu Lys Tyr Gly Gly Ser Gly Asp
50 55 60
Asp Gly Gly Asp Val Leu Ser Tyr Ile Leu Ala Val Glu Glu Leu Ala
65 70 75 80
Lys Tyr Asp Ala Gly Val Ala Ile Thr Leu Ser Ala Thr Val Ser Leu
85 90 95
Cys Ala Asn Pro Ile Trp Gln Phe Gly Thr Glu Ala Gln Lys Glu Lys
100 105 110
Phe Leu Val Pro Leu Val Glu Gly Thr Lys Leu Gly Ala Phe Gly Leu
115 120 125
Thr Glu Pro Asn Ala Gly Thr Asp Ala Ser Gly Gln Gln Thr Ile Ala
130 135 140
Thr Lys Asn Asp Asp Gly Thr Tyr Thr Leu Asn Gly Ser Lys Ile Phe
145 150 155 160
Ile Thr Asn Gly Gly Ala Ala Asp Ile Tyr Ile Val Phe Ala Met Thr
165 170 175
Asp Lys Ser Lys Gly Asn His Gly Ile Thr Ala Phe Ile Leu Glu Asp
180 185 190
Gly Thr Pro Gly Phe Thr Tyr Gly Lys Lys Glu Asp Lys Met Gly Ile
195 200 205
His Thr Ser Gln Thr Met Glu Leu Val Phe Gln Asp Val Lys Val Pro
210 215 220
Ala Glu Asn Met Leu Gly Glu Glu Gly Lys Gly Phe Lys Ile Ala Met
225 230 235 240
Met Thr Leu Asp Gly Gly Arg Ile Gly Val Ala Ala Gln Ala Leu Gly
245 250 255
Ile Ala Glu Ala Ala Leu Ala Asp Ala Val Glu Tyr Ser Lys Gln Arg
260 265 270
Val Gln Phe Gly Lys Pro Leu Cys Lys Phe Gln Ser Ile Ser Phe Lys
275 280 285
Leu Ala Asp Met Lys Met Gln Ile Glu Ala Ala Arg Asn Leu Val Tyr
290 295 300
Lys Ala Ala Cys Lys Lys Gln Glu Gly Lys Pro Phe Thr Val Asp Ala
305 310 315 320
Ala Ile Ala Lys Arg Val Ala Ser Asp Val Ala Met Arg Val Thr Thr
325 330 335
Glu Ala Val Gln Ile Phe Gly Gly Tyr Gly Tyr Ser Glu Glu Tyr Pro
340 345 350
Val Ala Arg His Met Arg Asp Ala Lys Ile Thr Gln Ile Tyr Glu Gly
355 360 365
Thr Asn Glu Val Gln Leu Met Val Thr Gly Gly Ala Leu Leu Arg Glu
370 375 380
Thr
385
<210> 106
<211> 1421
<212> DNA
<213> Artificial
<220>
<223> Acinetobacter sp. (strain ADP1), Acyl-CoA dehydrogenase;
EC=1.3.99 (Q937T2)
<400> 106
ggtacctttt ttgggctaac aggaggaatt aaccatgatc aacaagatca tcaacgatat 60
cgaaccgatt ctgaaatcta tcccagacgg ttctaccatc atgacttctg gcttcggtac 120
taccggtcag ccggaagcgc tgctggaagc gctgatcgac ttcgcaccga aagagctgac 180
tatcatcaac aacaacgctt cttccggtcc gaacggtctg actcagctgt tcactgctgg 240
tctggttaag aaactgatct gctcctaccc gaaatctatc tcttccaccg ttttcccgga 300
cctgtaccgc gctggtaaga tcgaactgga actggttccg cagggtaacc tggcttgccg 360
tatccaggca gctggcgcgg gtctgggtgc agtattcact ccgactggtt acggtactaa 420
gatcgctgaa ggtaaagaaa ctcgtatcat caacggtaaa aactacgttc tggaataccc 480
gctggaagct gactacgcat tcatctacgc tgacaaagct gaccgctggg gtaacctgac 540
ttaccgtaaa gcggcacgta acttcggtcc gatcatggcg aaagcagcta aaaccactat 600
tgctcaggtt aaccagactg ttgaactggg cgatctggac ccggaatgca tcatcactcc 660
gggtatcttc gtacagcacg ttgttcgtct gggtgacatc aaataatttt ttgggctaac 720
aggaggaatt aaccatgact attcagaagc gttctcgcga agatatcgct atcatgatcg 780
ctaaagacat cccggacggt tcttacgtta acctgggtat cggtctgccg actcacgttg 840
ctaaatatct gccgaaagac aaagaaatct tcctgcactc tgaaaacggt gttctggcat 900
tcggtccgcc gccagcagaa ggtgaagaag atcaggacct ggttaacgct ggtaaagagc 960
tggtaactct gctgtctggt ggttgcttca tgcaccacgg tgactccttc gacatcatgc 1020
gtggtggtca cctggatatc tgcgttatcg gtgcattcca ggttgcgctg aacggtgacc 1080
tggctaactg gcacaccggt aaagacgacg acgttccggc agtaggtggt gcaatggacc 1140
tggctgttgg tgcgaagcgt atcttcgttt acatggaaca caccaccaag aaaggtgaac 1200
cgaaaatcgt taagcacctg acttacccga tcaccggtga acagtgcgta gaccgtatct 1260
acactgacct gtgcaccatc gaactgaaag acggtcaggc ttacgttatc gaaatggttg 1320
acggtctgga tttcgacact ctgcaggcgc tgactgaatg cccgctgatc gaccactgca 1380
cctactcttc tctgattcag ctcagataag gatccgagct c 1421
<210> 107
<211> 223
<212> PRT
<213> Acinetobacter sp. (strain ADP1), Acyl-CoA dehydrogenase; EC=1.3.99(Q937T2)
<400> 107
Met Ile Asn Lys Ile Ile Asn Asp Ile Glu Pro Ile Leu Lys Ser Ile
1 5 10 15
Pro Asp Gly Ser Thr Ile Met Thr Ser Gly Phe Gly Thr Thr Gly Gln
20 25 30
Pro Glu Ala Leu Leu Glu Ala Leu Ile Asp Phe Ala Pro Lys Glu Leu
35 40 45
Thr Ile Ile Asn Asn Asn Ala Ser Ser Gly Pro Asn Gly Leu Thr Gln
50 55 60
Leu Phe Thr Ala Gly Leu Val Lys Lys Leu Ile Cys Ser Tyr Pro Lys
65 70 75 80
Ser Ile Ser Ser Thr Val Phe Pro Asp Leu Tyr Arg Ala Gly Lys Ile
85 90 95
Glu Leu Glu Leu Val Pro Gln Gly Asn Leu Ala Cys Arg Ile Gln Ala
100 105 110
Ala Gly Ala Gly Leu Gly Ala Val Phe Thr Pro Thr Gly Tyr Gly Thr
115 120 125
Lys Ile Ala Glu Gly Lys Glu Thr Arg Ile Ile Asn Gly Lys Asn Tyr
130 135 140
Val Leu Glu Tyr Pro Leu Glu Ala Asp Tyr Ala Phe Ile Tyr Ala Asp
145 150 155 160
Lys Ala Asp Arg Trp Gly Asn Leu Thr Tyr Arg Lys Ala Ala Arg Asn
165 170 175
Phe Gly Pro Ile Met Ala Lys Ala Ala Lys Thr Thr Ile Ala Gln Val
180 185 190
Asn Gln Thr Val Glu Leu Gly Asp Leu Asp Pro Glu Cys Ile Ile Thr
195 200 205
Pro Gly Ile Phe Val Gln His Val Val Arg Leu Gly Asp Ile Lys
210 215 220
<210> 108
<211> 1190
<212> DNA
<213> Artificial
<220>
<223> Deinococcus radiodurans, Acyl-CoA dehydrogenase (Q9RVV0)
<400> 108
ggtaccggat ccctaacagg aggaattaac catggacttc aatctgccgg acgatctgcg 60
tgaagttcag gcaactatcc gtgacttcat gctgactcgc gttgaagatc gcgctcagga 120
aatcgaacac accaactccg taccgccgga actgatcaaa gaagcggcag acctgggtct 180
gttcggtctg tctatcccgg aagaatacgg tggtgttggt ctgtcctctc tgggtcgttg 240
cgcagtttac gaagcgatgg gtcagggtca catgggcttc ggtggtatga tctccgcaca 300
cgcttctatc ggtacttctg gtctggttaa actgggtaac gaagagcaga agcagcgttt 360
cctgccgcgt atggcggcag gcgaatgcat cgctggcttc gctatcactg aaccaagctc 420
cggttctgac gctggtaaca tccgtactaa agctgttaag aaaggcgacg tttacgttct 480
gaacggtact aagcactaca tctccaacgc tccgatcgct ggtctgctga ccgttatcgc 540
tatcactgac ccggcacagg gttcccgtgg tatgtccgca ttcctggttg aaccgcagtc 600
tactccgggc gtatctatcg gtaaaatcga cgaaaaaatg ggtcagaaag gtgcgctgtc 660
tgcagaagtt atcttcgaag atgctgaaat cccggctgct aaccttctcg gtccggaaaa 720
ccgcggttac cgtgaagcgc tgggtattct gactaacggt cgcgttggta tcgctgcacg 780
ttctactggc gcgatgcagc gtctgctgga cctgtctgtt gcacacgctc agactcgcga 840
gcagttcggt aagccgatcg ctgagttcca ggcggtacag ttcatgctgg cagaaatgga 900
agttgcgatt cagacttctc gcgtactgtg gcagaaagtt gcatggatgg ttgaccaggg 960
tcaggacgtt aagcgtatgg cgtctgttgc taaataccac gcaactgaaa tgctgtctca 1020
ggttgctgac aaagcggtac aggttgctgg cggtatgggt tacgttaaag acgctccgtt 1080
cgaacgtttc taccgcgacc agcgtctgct gcgtatctac gaaggtactt ctgaaatcca 1140
gaaagttatc atcgctgctg aactgctgcg tcgcgagctc taataagctt 1190
<210> 109
<211> 383
<212> PRT
<213> Deinococcus radiodurans, Acyl-CoA dehydrogenase (Q9RVV0)
<400> 109
Met Asp Phe Asn Leu Pro Asp Asp Leu Arg Glu Val Gln Ala Thr Ile
1 5 10 15
Arg Asp Phe Met Leu Thr Arg Val Glu Asp Arg Ala Gln Glu Ile Glu
20 25 30
His Thr Asn Ser Val Pro Pro Glu Leu Ile Lys Glu Ala Ala Asp Leu
35 40 45
Gly Leu Phe Gly Leu Ser Ile Pro Glu Glu Tyr Gly Gly Val Gly Leu
50 55 60
Ser Ser Leu Gly Arg Cys Ala Val Tyr Glu Ala Met Gly Gln Gly His
65 70 75 80
Met Gly Phe Gly Gly Met Ile Ser Ala His Ala Ser Ile Gly Thr Ser
85 90 95
Gly Leu Val Lys Leu Gly Asn Glu Glu Gln Lys Gln Arg Phe Leu Pro
100 105 110
Arg Met Ala Ala Gly Glu Cys Ile Ala Gly Phe Ala Ile Thr Glu Pro
115 120 125
Ser Ser Gly Ser Asp Ala Gly Asn Ile Arg Thr Lys Ala Val Lys Lys
130 135 140
Gly Asp Val Tyr Val Leu Asn Gly Thr Lys His Tyr Ile Ser Asn Ala
145 150 155 160
Pro Ile Ala Gly Leu Leu Thr Val Ile Ala Ile Thr Asp Pro Ala Gln
165 170 175
Gly Ser Arg Gly Met Ser Ala Phe Leu Val Glu Pro Gln Ser Thr Pro
180 185 190
Gly Val Ser Ile Gly Lys Ile Asp Glu Lys Met Gly Gln Lys Gly Ala
195 200 205
Leu Ser Ala Glu Val Ile Phe Glu Asp Ala Glu Ile Pro Ala Ala Asn
210 215 220
Leu Leu Gly Pro Glu Asn Arg Gly Tyr Arg Glu Ala Leu Gly Ile Leu
225 230 235 240
Thr Asn Gly Arg Val Gly Ile Ala Ala Arg Ser Thr Gly Ala Met Gln
245 250 255
Arg Leu Leu Asp Leu Ser Val Ala His Ala Gln Thr Arg Glu Gln Phe
260 265 270
Gly Lys Pro Ile Ala Glu Phe Gln Ala Val Gln Phe Met Leu Ala Glu
275 280 285
Met Glu Val Ala Ile Gln Thr Ser Arg Val Leu Trp Gln Lys Val Ala
290 295 300
Trp Met Val Asp Gln Gly Gln Asp Val Lys Arg Met Ala Ser Val Ala
305 310 315 320
Lys Tyr His Ala Thr Glu Met Leu Ser Gln Val Ala Asp Lys Ala Val
325 330 335
Gln Val Ala Gly Gly Met Gly Tyr Val Lys Asp Ala Pro Phe Glu Arg
340 345 350
Phe Tyr Arg Asp Gln Arg Leu Leu Arg Ile Tyr Glu Gly Thr Ser Glu
355 360 365
Ile Gln Lys Val Ile Ile Ala Ala Glu Leu Leu Arg Arg Glu Thr
370 375 380
<210> 110
<211> 1202
<212> DNA
<213> Artificial
<220>
<223> Arabidopsis thaliana, Isovaleryl-CoA dehydrogenase,
mitochondrial; Short=IVD; EC=1.3.99.10
<400> 110
ggtaccggat ccctaacagg aggaattaac catggcctct tctctgctgt tcgacgacac 60
tcagctgcag ttcaaagaat ccgtttccaa gttcgctcag gacaacatcg ctccgcacgc 120
tgaacgtatc gacaaaacta actccttccc gaaagacgtt aacctgtgga aactgatggg 180
cgagttcaat ctgcacggta tcactgcgcc agaagaatac ggtggtctgg gtctgggtta 240
cctgtaccac tgcatcgcga tggaagaaat ctctcgcgct tctggttctg ttgctctgtc 300
ttacggcgca cactctaacc tgtgcatcaa ccagctggta cgtaacggta ctgctgcaca 360
gaaagagaag tatctgccga aactgatctc cggtgaacac gttggtgcac tggcgatgtc 420
tgaaccgaac gctggttctg acgttgttgg tatgaagtgc aaagcagaaa aagttgacgg 480
tggttacatt ctgaacggta acaagatgtg gtgcaccaac ggtccgtctg ctgaaactct 540
ggttgtttac gctaaaactg acaccaaagc gggttctaaa ggtatcactg cattcatcat 600
cgaaaaaggt atgaccggtt tctccactgc tcagaagctg gacaagctgg gtatgcgcgg 660
ttctgacact tgcgaactgg tattcgaaaa ctgcttcgta ccggaagaaa acatcctgga 720
taaagaaggt aaaggcgttt acgttctgat gtccggtctg gatctggaac gtctggtact 780
ggcagctggt ccgctgggta tcatgcaggc ttgcctggat aacgtactgc cgtacatccg 840
tcagcgtgaa cagttcggtc gtccggttgg cgagttccag ttcatccagg gtaaagttgc 900
tgacatgtac actgcgctgc agtcctcccg ttcttacgtt tactccgttg ctcgcgactg 960
cgacaacggt aaagttgatc cgaaagactg cgcaggcact atcctgtgcg cagctgaacg 1020
tgcaactcag gttgcgctgc aggcgattca gtgcctgggt ggtaacggtt acatcaacga 1080
atacgcaact ggtcgtctgc tgcgcgacgc gaagctgtac gaaatcggtg ctggtacttc 1140
tgaaatccgc cgtatcgtta tcggtcgtga actgttcaaa gaagaagagc tctaataagc 1200
tt 1202
<210> 111
<211> 387
<212> PRT
<213> Arabidopsis thaliana, Isovaleryl-CoA dehydrogenase, mitochondrial;Short=IVD; EC=1.3.99.10
<400> 111
Met Ala Ser Ser Leu Leu Phe Asp Asp Thr Gln Leu Gln Phe Lys Glu
1 5 10 15
Ser Val Ser Lys Phe Ala Gln Asp Asn Ile Ala Pro His Ala Glu Arg
20 25 30
Ile Asp Lys Thr Asn Ser Phe Pro Lys Asp Val Asn Leu Trp Lys Leu
35 40 45
Met Gly Glu Phe Asn Leu His Gly Ile Thr Ala Pro Glu Glu Tyr Gly
50 55 60
Gly Leu Gly Leu Gly Tyr Leu Tyr His Cys Ile Ala Met Glu Glu Ile
65 70 75 80
Ser Arg Ala Ser Gly Ser Val Ala Leu Ser Tyr Gly Ala His Ser Asn
85 90 95
Leu Cys Ile Asn Gln Leu Val Arg Asn Gly Thr Ala Ala Gln Lys Glu
100 105 110
Lys Tyr Leu Pro Lys Leu Ile Ser Gly Glu His Val Gly Ala Leu Ala
115 120 125
Met Ser Glu Pro Asn Ala Gly Ser Asp Val Val Gly Met Lys Cys Lys
130 135 140
Ala Glu Lys Val Asp Gly Gly Tyr Ile Leu Asn Gly Asn Lys Met Trp
145 150 155 160
Cys Thr Asn Gly Pro Ser Ala Glu Thr Leu Val Val Tyr Ala Lys Thr
165 170 175
Asp Thr Lys Ala Gly Ser Lys Gly Ile Thr Ala Phe Ile Ile Glu Lys
180 185 190
Gly Met Thr Gly Phe Ser Thr Ala Gln Lys Leu Asp Lys Leu Gly Met
195 200 205
Arg Gly Ser Asp Thr Cys Glu Leu Val Phe Glu Asn Cys Phe Val Pro
210 215 220
Glu Glu Asn Ile Leu Asp Lys Glu Gly Lys Gly Val Tyr Val Leu Met
225 230 235 240
Ser Gly Leu Asp Leu Glu Arg Leu Val Leu Ala Ala Gly Pro Leu Gly
245 250 255
Ile Met Gln Ala Cys Leu Asp Asn Val Leu Pro Tyr Ile Arg Gln Arg
260 265 270
Glu Gln Phe Gly Arg Pro Val Gly Glu Phe Gln Phe Ile Gln Gly Lys
275 280 285
Val Ala Asp Met Tyr Thr Ala Leu Gln Ser Ser Arg Ser Tyr Val Tyr
290 295 300
Ser Val Ala Arg Asp Cys Asp Asn Gly Lys Val Asp Pro Lys Asp Cys
305 310 315 320
Ala Gly Thr Ile Leu Cys Ala Ala Glu Arg Ala Thr Gln Val Ala Leu
325 330 335
Gln Ala Ile Gln Cys Leu Gly Gly Asn Gly Tyr Ile Asn Glu Tyr Ala
340 345 350
Thr Gly Arg Leu Leu Arg Asp Ala Lys Leu Tyr Glu Ile Gly Ala Gly
355 360 365
Thr Ser Glu Ile Arg Arg Ile Val Ile Gly Arg Glu Leu Phe Lys Glu
370 375 380
Glu Glu Thr
385
<210> 112
<211> 1232
<212> DNA
<213> Artificial
<220>
<223> Homo sapiens, Glutaryl-CoA dehydrogenase, mitochondrial;
Short=GCD; EC=1.3.99.7 (Q92947)
<400> 112
ggtaccggat ccctaacagg aggaattaac catgggtccg gaatttgact ggcaggaccc 60
gctggtactg gaagagcagc tgaccactga cgaaatcctg atccgtgaca ccttccgtac 120
ttactgccag gaacgtctga tgccgcgcat tctgctggct aaccgtaacg aagttttcca 180
ccgtgaaatc atctctgaaa tgggtgaact gggtgttctg ggtccgacta tcaaaggtta 240
cggttgcgca ggcgtttctt ccgttgctta cggtctgctg gcgcgtgaac tggaacgcgt 300
tgactccggt taccgttctg caatgtccgt tcagtcctct ctggtaatgc acccaatcta 360
cgcttacggt tctgaagagc agcgtcagaa atatctgccg cagctggcga aaggtgaact 420
gctgggttgc ttcggtctga ctgaaccgaa ctccggttct gacccgtcct ctatggaaac 480
tcgcgcacac tacaactctt ctaacaagtc ttacactctg aacggcacca aaacctggat 540
cactaactct ccgatggctg acctgttcgt tgtatgggct cgctgcgaag atggttgcat 600
ccgcggtttc ctgctggaaa aaggtatgcg tggtctgtct gcaccgcgta tccagggtaa 660
gttctctctg cgcgcttctg caactggtat gatcatcatg gacggtgttg aagttccgga 720
agaaaacgtt ctgccgggtg catcttctct gggtggtccg ttcggttgcc tgaacaacgc 780
gcgttacggt atcgcatggg gcgtactggg tgcttctgag ttctgcctgc acactgcacg 840
tcagtacgca ctggatcgca tgcagttcgg tgttccgctg gcgcgtaacc agctgattca 900
gaagaaactg gctgacatgc tgactgaaat cactctgggt ctgcacgctt gcctgcagct 960
gggtcgtctg aaagaccagg acaaagctgc accggaaatg gtttctctgc tgaagcgtaa 1020
caactgcggt aaagcgctgg atatcgctcg ccaggctcgc gacatgctgg gtggtaacgg 1080
tatctctgac gaataccacg ttatccgtca cgcgatgaac ctggaagctg ttaacaccta 1140
cgaaggtact cacgacatcc acgcgctgat cctgggtcgt gctatcaccg gtatccaggc 1200
tttcactgct tctaaagagc tctaataagc tt 1232
<210> 113
<211> 397
<212> PRT
<213> Homo sapiens, Glutaryl-CoA dehydrogenase, mitochondrial; Short=GCD;EC=1.3.99.7 (Q92947)
<400> 113
Met Gly Pro Glu Phe Asp Trp Gln Asp Pro Leu Val Leu Glu Glu Gln
1 5 10 15
Leu Thr Thr Asp Glu Ile Leu Ile Arg Asp Thr Phe Arg Thr Tyr Cys
20 25 30
Gln Glu Arg Leu Met Pro Arg Ile Leu Leu Ala Asn Arg Asn Glu Val
35 40 45
Phe His Arg Glu Ile Ile Ser Glu Met Gly Glu Leu Gly Val Leu Gly
50 55 60
Pro Thr Ile Lys Gly Tyr Gly Cys Ala Gly Val Ser Ser Val Ala Tyr
65 70 75 80
Gly Leu Leu Ala Arg Glu Leu Glu Arg Val Asp Ser Gly Tyr Arg Ser
85 90 95
Ala Met Ser Val Gln Ser Ser Leu Val Met His Pro Ile Tyr Ala Tyr
100 105 110
Gly Ser Glu Glu Gln Arg Gln Lys Tyr Leu Pro Gln Leu Ala Lys Gly
115 120 125
Glu Leu Leu Gly Cys Phe Gly Leu Thr Glu Pro Asn Ser Gly Ser Asp
130 135 140
Pro Ser Ser Met Glu Thr Arg Ala His Tyr Asn Ser Ser Asn Lys Ser
145 150 155 160
Tyr Thr Leu Asn Gly Thr Lys Thr Trp Ile Thr Asn Ser Pro Met Ala
165 170 175
Asp Leu Phe Val Val Trp Ala Arg Cys Glu Asp Gly Cys Ile Arg Gly
180 185 190
Phe Leu Leu Glu Lys Gly Met Arg Gly Leu Ser Ala Pro Arg Ile Gln
195 200 205
Gly Lys Phe Ser Leu Arg Ala Ser Ala Thr Gly Met Ile Ile Met Asp
210 215 220
Gly Val Glu Val Pro Glu Glu Asn Val Leu Pro Gly Ala Ser Ser Leu
225 230 235 240
Gly Gly Pro Phe Gly Cys Leu Asn Asn Ala Arg Tyr Gly Ile Ala Trp
245 250 255
Gly Val Leu Gly Ala Ser Glu Phe Cys Leu His Thr Ala Arg Gln Tyr
260 265 270
Ala Leu Asp Arg Met Gln Phe Gly Val Pro Leu Ala Arg Asn Gln Leu
275 280 285
Ile Gln Lys Lys Leu Ala Asp Met Leu Thr Glu Ile Thr Leu Gly Leu
290 295 300
His Ala Cys Leu Gln Leu Gly Arg Leu Lys Asp Gln Asp Lys Ala Ala
305 310 315 320
Pro Glu Met Val Ser Leu Leu Lys Arg Asn Asn Cys Gly Lys Ala Leu
325 330 335
Asp Ile Ala Arg Gln Ala Arg Asp Met Leu Gly Gly Asn Gly Ile Ser
340 345 350
Asp Glu Tyr His Val Ile Arg His Ala Met Asn Leu Glu Ala Val Asn
355 360 365
Thr Tyr Glu Gly Thr His Asp Ile His Ala Leu Ile Leu Gly Arg Ala
370 375 380
Ile Thr Gly Ile Gln Ala Phe Thr Ala Ser Lys Glu Thr
385 390 395
<210> 114
<211> 1784
<212> DNA
<213> Artificial
<220>
<223> Homo sapiens, Electron transfer flavoprotein subunit alpha,
mitochondrial (P13804)
<400> 114
ggtaccggat ccctaacagg aggaattaat catgaccact ctggttatcg ctgaacacgc 60
taacgactct ctggcaccaa tcactctgaa caccatcact gcggcaactc gtctgggtgg 120
tgaagtttcc tgcctggttg ctggcaccaa gtgcgacaaa gttgctcagg acctgtgcaa 180
agtagcgggt atcgcgaaag ttctggtagc gcagcacgac gtttacaaag gtctgctgcc 240
ggaagagctg actccgctga tcctggcaac tcagaagcag ttcaactaca ctcacatctg 300
tgcgggtgct tctgcattcg gtaaaaatct gctgccgcgc gttgctgcga aactggaagt 360
tgcaccgatt tctgacatca tcgctatcaa gtctccggat accttcgtac gtactatcta 420
cgctggtaac gcactgtgca ccgttaagtg cgacgaaaaa gttaaagtat tctctgtacg 480
tggtacttct ttcgacgctg cagcaacttc tggtggttct gcatcttctg aaaaagcatc 540
ttccacttct ccggttgaaa tctctgagtg gctggatcag aaactgacca agtctgaccg 600
tccggaactg actggcgcga aagttgttgt ttctggtggt cgtggtctga aatctggtga 660
aaacttcaaa ctgctgtacg acctggctga ccagctgcac gctgctgttg gtgcttctcg 720
cgctgctgtt gatgctggct tcgtaccgaa cgacatgcaa gttggtcaga ctggtaaaat 780
cgttgcaccg gaactgtaca tcgctgttgg tatctccggt gcgattcagc acctggcggg 840
tatgaaagac tccaaaacta tcgttgctat caacaaagac ccggaagcac cgatcttcca 900
ggttgctgac tacggtatcg ttgctgacct gttcaaagtt gttccggaaa tgactgaaat 960
cctgaagaag aagtaataac tagtctaaca ggaggaatta accatggctg aactgcgcgt 1020
actggttgct gttaagcgcg ttatcgacta cgcagttaaa atccgcgtta agccggaccg 1080
tactggcgtt gttactgacg gtgttaaaca ctctatgaac ccgttctgcg aaatcgctgt 1140
tgaagaagcg gtacgtctga aagagaagaa actggttaaa gaagttatcg ctgtttcctg 1200
cggtccggca cagtgccagg aaaccattcg tactgcactg gcgatgggcg cagaccgcgg 1260
tatccacgtt gaagttccgc cagctgaagc agaacgtctg ggtccgctcc aggttgctcg 1320
cgtactggcg aaactggctg aaaaagaaaa agttgacctg gtactgctgg gtaagcaggc 1380
tatcgacgac gactgcaacc agactggtca gatgactgct ggcttcctgg actggccgca 1440
gggtactttc gcttctcagg taactctgga aggtgacaaa ctgaaagttg aacgtgaaat 1500
cgacggtggt ctggaaactc tgcgtctgaa actgccggca gttgttactg ctgatctgcg 1560
tctgaacgaa ccgcgttacg caactctgcc gaacattatg aaagcgaaga agaagaaaat 1620
cgaagttatc aagccgggcg acctgggtgt tgacctgact tctaaactgt ctgttatctc 1680
cgttgaagat ccgccgcagc gtactgctgg cgttaaagtt gaaaccactg aggatctggt 1740
agcgaaactg aaagaaatcg gtcgtatcga gctctaataa gctt 1784
<210> 115
<211> 314
<212> PRT
<213> Homo sapiens, Electron transfer flavoprotein subunit alpha,mitochondrial (P13804)
<400> 115
Met Thr Thr Leu Val Ile Ala Glu His Ala Asn Asp Ser Leu Ala Pro
1 5 10 15
Ile Thr Leu Asn Thr Ile Thr Ala Ala Thr Arg Leu Gly Gly Glu Val
20 25 30
Ser Cys Leu Val Ala Gly Thr Lys Cys Asp Lys Val Ala Gln Asp Leu
35 40 45
Cys Lys Val Ala Gly Ile Ala Lys Val Leu Val Ala Gln His Asp Val
50 55 60
Tyr Lys Gly Leu Leu Pro Glu Glu Leu Thr Pro Leu Ile Leu Ala Thr
65 70 75 80
Gln Lys Gln Phe Asn Tyr Thr His Ile Cys Ala Gly Ala Ser Ala Phe
85 90 95
Gly Lys Asn Leu Leu Pro Arg Val Ala Ala Lys Leu Glu Val Ala Pro
100 105 110
Ile Ser Asp Ile Ile Ala Ile Lys Ser Pro Asp Thr Phe Val Arg Thr
115 120 125
Ile Tyr Ala Gly Asn Ala Leu Cys Thr Val Lys Cys Asp Glu Lys Val
130 135 140
Lys Val Phe Ser Val Arg Gly Thr Ser Phe Asp Ala Ala Ala Thr Ser
145 150 155 160
Gly Gly Ser Ala Ser Ser Glu Lys Ala Ser Ser Thr Ser Pro Val Glu
165 170 175
Ile Ser Glu Trp Leu Asp Gln Lys Leu Thr Lys Ser Asp Arg Pro Glu
180 185 190
Leu Thr Gly Ala Lys Val Val Val Ser Gly Gly Arg Gly Leu Lys Ser
195 200 205
Gly Glu Asn Phe Lys Leu Leu Tyr Asp Leu Ala Asp Gln Leu His Ala
210 215 220
Ala Val Gly Ala Ser Arg Ala Ala Val Asp Ala Gly Phe Val Pro Asn
225 230 235 240
Asp Met Gln Val Gly Gln Thr Gly Lys Ile Val Ala Pro Glu Leu Tyr
245 250 255
Ile Ala Val Gly Ile Ser Gly Ala Ile Gln His Leu Ala Gly Met Lys
260 265 270
Asp Ser Lys Thr Ile Val Ala Ile Asn Lys Asp Pro Glu Ala Pro Ile
275 280 285
Phe Gln Val Ala Asp Tyr Gly Ile Val Ala Asp Leu Phe Lys Val Val
290 295 300
Pro Glu Met Thr Glu Ile Leu Lys Lys Lys
305 310
<210> 116
<211> 255
<212> PRT
<213> Homo sapiens, Electron transfer flavoprotein subunit beta (P38117)
<400> 116
Met Ala Glu Leu Arg Val Leu Val Ala Val Lys Arg Val Ile Asp Tyr
1 5 10 15
Ala Val Lys Ile Arg Val Lys Pro Asp Arg Thr Gly Val Val Thr Asp
20 25 30
Gly Val Lys His Ser Met Asn Pro Phe Cys Glu Ile Ala Val Glu Glu
35 40 45
Ala Val Arg Leu Lys Glu Lys Lys Leu Val Lys Glu Val Ile Ala Val
50 55 60
Ser Cys Gly Pro Ala Gln Cys Gln Glu Thr Ile Arg Thr Ala Leu Ala
65 70 75 80
Met Gly Ala Asp Arg Gly Ile His Val Glu Val Pro Pro Ala Glu Ala
85 90 95
Glu Arg Leu Gly Pro Leu Gln Val Ala Arg Val Leu Ala Lys Leu Ala
100 105 110
Glu Lys Glu Lys Val Asp Leu Val Leu Leu Gly Lys Gln Ala Ile Asp
115 120 125
Asp Asp Cys Asn Gln Thr Gly Gln Met Thr Ala Gly Phe Leu Asp Trp
130 135 140
Pro Gln Gly Thr Phe Ala Ser Gln Val Thr Leu Glu Gly Asp Lys Leu
145 150 155 160
Lys Val Glu Arg Glu Ile Asp Gly Gly Leu Glu Thr Leu Arg Leu Lys
165 170 175
Leu Pro Ala Val Val Thr Ala Asp Leu Arg Leu Asn Glu Pro Arg Tyr
180 185 190
Ala Thr Leu Pro Asn Ile Met Lys Ala Lys Lys Lys Lys Ile Glu Val
195 200 205
Ile Lys Pro Gly Asp Leu Gly Val Asp Leu Thr Ser Lys Leu Ser Val
210 215 220
Ile Ser Val Glu Asp Pro Pro Gln Arg Thr Ala Gly Val Lys Val Glu
225 230 235 240
Thr Thr Glu Asp Leu Val Ala Lys Leu Lys Glu Ile Gly Arg Ile
245 250 255
<210> 117
<211> 316
<212> PRT
<213> Homo sapiens Acyl-coenzyme A thioesterase 8, EC=3.1.2.27 (O14734)
<400> 117
Met Ser Ser Pro Gln Ala Pro Glu Asp Gly Gln Gly Cys Gly Asp Arg
1 5 10 15
Gly Asp Pro Pro Gly Asp Leu Arg Ser Val Leu Val Thr Thr Val Leu
20 25 30
Asn Leu Glu Pro Leu Asp Glu Asp Leu Phe Arg Gly Arg His Tyr Trp
35 40 45
Val Pro Ala Lys Arg Leu Phe Gly Gly Gln Ile Val Gly Gln Ala Leu
50 55 60
Val Ala Ala Ala Lys Ser Val Ser Glu Asp Val His Val His Ser Leu
65 70 75 80
His Cys Tyr Phe Val Arg Ala Gly Asp Pro Lys Leu Pro Val Leu Tyr
85 90 95
Gln Val Glu Arg Thr Arg Thr Gly Ser Ser Phe Ser Val Arg Ser Val
100 105 110
Lys Ala Val Gln His Gly Lys Pro Ile Phe Ile Cys Gln Ala Ser Phe
115 120 125
Gln Gln Ala Gln Pro Ser Pro Met Gln His Gln Phe Ser Met Pro Thr
130 135 140
Val Pro Pro Pro Glu Glu Leu Leu Asp Cys Glu Thr Leu Ile Asp Gln
145 150 155 160
Tyr Leu Arg Asp Pro Asn Leu Gln Lys Arg Tyr Pro Leu Ala Leu Asn
165 170 175
Arg Ile Ala Ala Gln Glu Val Pro Ile Glu Ile Lys Pro Val Asn Pro
180 185 190
Ser Pro Leu Ser Gln Leu Gln Arg Met Glu Pro Lys Gln Met Phe Trp
195 200 205
Val Arg Ala Arg Gly Tyr Ile Gly Glu Gly Asp Met Lys Met His Cys
210 215 220
Cys Val Ala Ala Tyr Ile Ser Asp Tyr Ala Phe Leu Gly Thr Ala Leu
225 230 235 240
Leu Pro His Gln Trp Gln His Lys Val His Phe Met Val Ser Leu Asp
245 250 255
His Ser Met Trp Phe His Ala Pro Phe Arg Ala Asp His Trp Met Leu
260 265 270
Tyr Glu Cys Glu Ser Pro Trp Ala Gly Gly Ser Arg Gly Leu Val His
275 280 285
Gly Arg Leu Trp Arg Gln Asp Gly Val Leu Ala Val Thr Cys Ala Gln
290 295 300
Glu Gly Val Ile Arg Val Lys Pro Gln Val Ser Glu
305 310 315
<210> 118
<211> 931
<212> DNA
<213> Artificial
<220>
<223> Hyphomonas neptunium Acyl-CoA thioesterase (Q0BZ30)
<400> 118
ggtacctttt ttgggctaac aggaggaatt aaccatggcg aaagacccgg ttggcgatct 60
gctgactctg ctggatctgg aacgtctgga actggatctg ttccgcggtc agtctccgga 120
cgaagaagaa tctcagcgcg tattcggtgg tcaggttatc gctcagtctc tggttgctgc 180
ttaccgtact gtaccggacg accgtctgtg ccactctctg cactgctact tcatccgtcc 240
gggcgacccg tctgtaccaa tcatctacca ggttgaccac tctcgcgacg gtggttcttt 300
caccactcgt cgcgttgttg ctatccagca cggtaagcag attttcaacc tggcagcttc 360
tttccacgtt gttgaagatt cctggcacca ccagcacgaa atgccggaag ttgacccgcc 420
agaatccgta ggtgaccgta tcgagtggcg tcgtaaattc gctgaacagg ttccggaacg 480
tcaccgcggt cacttcctgc gcgaccgtcc ggttgaaatg cgtgaaatcg acccgctgga 540
cccgctgaaa ccggcaaaag cgtctgacca gcagaacctg tggttccgcg ttgctcgtcc 600
gatcgacgaa gcgccgtggc tgcaccactg cctgatggct tacgcttctg acatggcgct 660
gctgggtact ggtaaccgtc cgcacggtat ctcctggatg accggtgaac tgatgtccgc 720
atctctggat cacgcgatgt ggttccacgc accgactaaa ttcgacgagt ggcacctgta 780
ctctatggac tctccgtacg caggcggtgc acgttctttc aaccgcggtt ctatctacga 840
ctccaccggt cgtctggttg cttctgttgc tcaggaaggt ctgatgcgtc gcgttgaacg 900
taagccgcgt aaaagctaag gatccgagct c 931
<210> 119
<211> 294
<212> PRT
<213> Hyphomonas neptunium Acyl-CoA thioesterase (Q0BZ30)
<400> 119
Met Ala Lys Asp Pro Val Gly Asp Leu Leu Thr Leu Leu Asp Leu Glu
1 5 10 15
Arg Leu Glu Leu Asp Leu Phe Arg Gly Gln Ser Pro Asp Glu Glu Glu
20 25 30
Ser Gln Arg Val Phe Gly Gly Gln Val Ile Ala Gln Ser Leu Val Ala
35 40 45
Ala Tyr Arg Thr Val Pro Asp Asp Arg Leu Cys His Ser Leu His Cys
50 55 60
Tyr Phe Ile Arg Pro Gly Asp Pro Ser Val Pro Ile Ile Tyr Gln Val
65 70 75 80
Asp His Ser Arg Asp Gly Gly Ser Phe Thr Thr Arg Arg Val Val Ala
85 90 95
Ile Gln His Gly Lys Gln Ile Phe Asn Leu Ala Ala Ser Phe His Val
100 105 110
Val Glu Asp Ser Trp His His Gln His Glu Met Pro Glu Val Asp Pro
115 120 125
Pro Glu Ser Val Gly Asp Arg Ile Glu Trp Arg Arg Lys Phe Ala Glu
130 135 140
Gln Val Pro Glu Arg His Arg Gly His Phe Leu Arg Asp Arg Pro Val
145 150 155 160
Glu Met Arg Glu Ile Asp Pro Leu Asp Pro Leu Lys Pro Ala Lys Ala
165 170 175
Ser Asp Gln Gln Asn Leu Trp Phe Arg Val Ala Arg Pro Ile Asp Glu
180 185 190
Ala Pro Trp Leu His His Cys Leu Met Ala Tyr Ala Ser Asp Met Ala
195 200 205
Leu Leu Gly Thr Gly Asn Arg Pro His Gly Ile Ser Trp Met Thr Gly
210 215 220
Glu Leu Met Ser Ala Ser Leu Asp His Ala Met Trp Phe His Ala Pro
225 230 235 240
Thr Lys Phe Asp Glu Trp His Leu Tyr Ser Met Asp Ser Pro Tyr Ala
245 250 255
Gly Gly Ala Arg Ser Phe Asn Arg Gly Ser Ile Tyr Asp Ser Thr Gly
260 265 270
Arg Leu Val Ala Ser Val Ala Gln Glu Gly Leu Met Arg Arg Val Glu
275 280 285
Arg Lys Pro Arg Lys Ser
290
<210> 120
<211> 1421
<212> DNA
<213> Artificial
<220>
<223> Acinetobacter sp. (strain ADP1), Acyl-CoA-transferase subunit A
(EC=2.8.3.6) (Q937T0)
<400> 120
ggtacctttt ttgggctaac aggaggaatt aaccatgatc aacaagatca tcaacgatat 60
cgaaccgatt ctgaaatcta tcccagacgg ttctaccatc atgacttctg gcttcggtac 120
taccggtcag ccggaagcgc tgctggaagc gctgatcgac ttcgcaccga aagagctgac 180
tatcatcaac aacaacgctt cttccggtcc gaacggtctg actcagctgt tcactgctgg 240
tctggttaag aaactgatct gctcctaccc gaaatctatc tcttccaccg ttttcccgga 300
cctgtaccgc gctggtaaga tcgaactgga actggttccg cagggtaacc tggcttgccg 360
tatccaggca gctggcgcgg gtctgggtgc agtattcact ccgactggtt acggtactaa 420
gatcgctgaa ggtaaagaaa ctcgtatcat caacggtaaa aactacgttc tggaataccc 480
gctggaagct gactacgcat tcatctacgc tgacaaagct gaccgctggg gtaacctgac 540
ttaccgtaaa gcggcacgta acttcggtcc gatcatggcg aaagcagcta aaaccactat 600
tgctcaggtt aaccagactg ttgaactggg cgatctggac ccggaatgca tcatcactcc 660
gggtatcttc gtacagcacg ttgttcgtct gggtgacatc aaataatttt ttgggctaac 720
aggaggaatt aaccatgact attcagaagc gttctcgcga agatatcgct atcatgatcg 780
ctaaagacat cccggacggt tcttacgtta acctgggtat cggtctgccg actcacgttg 840
ctaaatatct gccgaaagac aaagaaatct tcctgcactc tgaaaacggt gttctggcat 900
tcggtccgcc gccagcagaa ggtgaagaag atcaggacct ggttaacgct ggtaaagagc 960
tggtaactct gctgtctggt ggttgcttca tgcaccacgg tgactccttc gacatcatgc 1020
gtggtggtca cctggatatc tgcgttatcg gtgcattcca ggttgcgctg aacggtgacc 1080
tggctaactg gcacaccggt aaagacgacg acgttccggc agtaggtggt gcaatggacc 1140
tggctgttgg tgcgaagcgt atcttcgttt acatggaaca caccaccaag aaaggtgaac 1200
cgaaaatcgt taagcacctg acttacccga tcaccggtga acagtgcgta gaccgtatct 1260
acactgacct gtgcaccatc gaactgaaag acggtcaggc ttacgttatc gaaatggttg 1320
acggtctgga tttcgacact ctgcaggcgc tgactgaatg cccgctgatc gaccactgca 1380
cctactcttc tctgattcag ctcagataag gatccgagct c 1421
<210> 121
<211> 223
<212> PRT
<213> Acinetobacter sp. (strain ADP1), Acyl-CoA-transferase subunit A (EC=2.8.3.6) (Q937T0)
<400> 121
Met Ile Asn Lys Ile Ile Asn Asp Ile Glu Pro Ile Leu Lys Ser Ile
1 5 10 15
Pro Asp Gly Ser Thr Ile Met Thr Ser Gly Phe Gly Thr Thr Gly Gln
20 25 30
Pro Glu Ala Leu Leu Glu Ala Leu Ile Asp Phe Ala Pro Lys Glu Leu
35 40 45
Thr Ile Ile Asn Asn Asn Ala Ser Ser Gly Pro Asn Gly Leu Thr Gln
50 55 60
Leu Phe Thr Ala Gly Leu Val Lys Lys Leu Ile Cys Ser Tyr Pro Lys
65 70 75 80
Ser Ile Ser Ser Thr Val Phe Pro Asp Leu Tyr Arg Ala Gly Lys Ile
85 90 95
Glu Leu Glu Leu Val Pro Gln Gly Asn Leu Ala Cys Arg Ile Gln Ala
100 105 110
Ala Gly Ala Gly Leu Gly Ala Val Phe Thr Pro Thr Gly Tyr Gly Thr
115 120 125
Lys Ile Ala Glu Gly Lys Glu Thr Arg Ile Ile Asn Gly Lys Asn Tyr
130 135 140
Val Leu Glu Tyr Pro Leu Glu Ala Asp Tyr Ala Phe Ile Tyr Ala Asp
145 150 155 160
Lys Ala Asp Arg Trp Gly Asn Leu Thr Tyr Arg Lys Ala Ala Arg Asn
165 170 175
Phe Gly Pro Ile Met Ala Lys Ala Ala Lys Thr Thr Ile Ala Gln Val
180 185 190
Asn Gln Thr Val Glu Leu Gly Asp Leu Asp Pro Glu Cys Ile Ile Thr
195 200 205
Pro Gly Ile Phe Val Gln His Val Val Arg Leu Gly Asp Ile Lys
210 215 220
<210> 122
<211> 224
<212> PRT
<213> Acinetobacter sp. (strain ADP1) Acyl-CoA-transferase subunit B (EC2.8.3.6) (Q937S9)
<400> 122
Met Thr Ile Gln Lys Arg Ser Arg Glu Asp Ile Ala Ile Met Ile Ala
1 5 10 15
Lys Asp Ile Pro Asp Gly Ser Tyr Val Asn Leu Gly Ile Gly Leu Pro
20 25 30
Thr His Val Ala Lys Tyr Leu Pro Lys Asp Lys Glu Ile Phe Leu His
35 40 45
Ser Glu Asn Gly Val Leu Ala Phe Gly Pro Pro Pro Ala Glu Gly Glu
50 55 60
Glu Asp Gln Asp Leu Val Asn Ala Gly Lys Glu Leu Val Thr Leu Leu
65 70 75 80
Ser Gly Gly Cys Phe Met His His Gly Asp Ser Phe Asp Ile Met Arg
85 90 95
Gly Gly His Leu Asp Ile Cys Val Ile Gly Ala Phe Gln Val Ala Leu
100 105 110
Asn Gly Asp Leu Ala Asn Trp His Thr Gly Lys Asp Asp Asp Val Pro
115 120 125
Ala Val Gly Gly Ala Met Asp Leu Ala Val Gly Ala Lys Arg Ile Phe
130 135 140
Val Tyr Met Glu His Thr Thr Lys Lys Gly Glu Pro Lys Ile Val Lys
145 150 155 160
His Leu Thr Tyr Pro Ile Thr Gly Glu Gln Cys Val Asp Arg Ile Tyr
165 170 175
Thr Asp Leu Cys Thr Ile Glu Leu Lys Asp Gly Gln Ala Tyr Val Ile
180 185 190
Glu Met Val Asp Gly Leu Asp Phe Asp Thr Leu Gln Ala Leu Thr Glu
195 200 205
Cys Pro Leu Ile Asp His Cys Thr Tyr Ser Ser Leu Ile Gln Leu Arg
210 215 220
<210> 123
<211> 437
<212> PRT
<213> Clostridium kluyveri (strain ATCC 8527) acetate CoA-transferaseactivity (A5N390)
<400> 123
Met Val Phe Lys Asn Trp Gln Asp Leu Tyr Lys Ser Lys Ile Val Ser
1 5 10 15
Ala Asp Glu Ala Val Ser Lys Val Ser Cys Gly Asp Ser Ile Ile Leu
20 25 30
Gly Asn Ala Cys Gly Ala Ser Leu Thr Leu Leu Asp Ala Leu Ala Ala
35 40 45
Asn Lys Glu Lys Tyr Lys Ser Val Lys Ile His Asn Leu Ile Leu Asn
50 55 60
Tyr Lys Asn Asp Ile Tyr Thr Asp Pro Glu Ser Glu Lys Tyr Ile His
65 70 75 80
Gly Asn Thr Phe Phe Val Ser Gly Gly Thr Lys Glu Ala Val Asn Cys
85 90 95
Asn Arg Thr Asp Tyr Thr Pro Cys Phe Phe Tyr Glu Ile Pro Lys Leu
100 105 110
Leu Lys Gln Lys Tyr Ile Asn Ala Asp Val Ala Phe Ile Gln Val Ser
115 120 125
Lys Pro Asp Ser His Gly Tyr Cys Ser Phe Gly Val Ser Thr Asp Tyr
130 135 140
Ser Gln Ala Met Val Gln Ser Ala Lys Leu Ile Ile Ala Glu Val Asn
145 150 155 160
Asp Gln Met Pro Arg Val Leu Gly Asp Asn Phe Ile His Ile Ser Asp
165 170 175
Met Asp Tyr Ile Val Glu Ser Ser Arg Pro Ile Leu Glu Leu Thr Pro
180 185 190
Pro Lys Ile Gly Glu Val Glu Lys Thr Ile Gly Lys Tyr Cys Ala Ser
195 200 205
Leu Val Glu Asp Gly Ser Thr Leu Gln Leu Gly Ile Gly Ala Ile Pro
210 215 220
Asp Ala Val Leu Leu Phe Leu Lys Asp Lys Lys Asp Leu Gly Ile His
225 230 235 240
Ser Glu Met Ile Ser Asp Gly Val Val Glu Leu Val Glu Ala Gly Val
245 250 255
Ile Thr Asn Lys Lys Lys Ser Leu His Pro Gly Lys Ile Ile Ile Thr
260 265 270
Phe Leu Met Gly Thr Lys Lys Leu Tyr Asp Phe Ile Asn Asp Asn Pro
275 280 285
Met Val Glu Gly Tyr Pro Val Asp Tyr Val Asn Asp Pro Lys Val Ile
290 295 300
Met Gln Asn Ser Lys Met Val Cys Ile Asn Ser Cys Val Glu Val Asp
305 310 315 320
Phe Thr Gly Gln Val Cys Ala Glu Ser Val Gly Phe Lys Gln Ile Ser
325 330 335
Gly Val Gly Gly Gln Val Asp Tyr Met Arg Gly Ala Ser Met Ala Asp
340 345 350
Gly Gly Lys Ser Ile Leu Ala Ile Pro Ser Thr Ala Ala Gly Gly Lys
355 360 365
Ile Ser Arg Ile Val Pro Ile Leu Thr Glu Gly Ala Gly Val Thr Thr
370 375 380
Ser Arg Tyr Asp Val Gln Tyr Val Val Thr Glu Tyr Gly Ile Ala Leu
385 390 395 400
Leu Lys Gly Lys Ser Ile Arg Glu Arg Ala Lys Glu Leu Ile Lys Ile
405 410 415
Ala His Pro Lys Phe Arg Glu Glu Leu Thr Ala Gln Phe Glu Lys Arg
420 425 430
Phe Ser Cys Lys Leu
435
<210> 124
<211> 538
<212> PRT
<213> Clostridium kluyveri (strain ATCC 8527) Succinyl-CoA:coenzyme Atransferase (P38946)
<400> 124
Met Ser Lys Gly Ile Lys Asn Ser Gln Leu Lys Lys Lys Asn Val Lys
1 5 10 15
Ala Ser Asn Val Ala Glu Lys Ile Glu Glu Lys Val Glu Lys Thr Asp
20 25 30
Lys Val Val Glu Lys Ala Ala Glu Val Thr Glu Lys Arg Ile Arg Asn
35 40 45
Leu Lys Leu Gln Glu Lys Val Val Thr Ala Asp Val Ala Ala Asp Met
50 55 60
Ile Glu Asn Gly Met Ile Val Ala Ile Ser Gly Phe Thr Pro Ser Gly
65 70 75 80
Tyr Pro Lys Glu Val Pro Lys Ala Leu Thr Lys Lys Val Asn Ala Leu
85 90 95
Glu Glu Glu Phe Lys Val Thr Leu Tyr Thr Gly Ser Ser Thr Gly Ala
100 105 110
Asp Ile Asp Gly Glu Trp Ala Lys Ala Gly Ile Ile Glu Arg Arg Ile
115 120 125
Pro Tyr Gln Thr Asn Ser Asp Met Arg Lys Lys Ile Asn Asp Gly Ser
130 135 140
Ile Lys Tyr Ala Asp Met His Leu Ser His Met Ala Gln Tyr Ile Asn
145 150 155 160
Tyr Ser Val Ile Pro Lys Val Asp Ile Ala Ile Ile Glu Ala Val Ala
165 170 175
Ile Thr Glu Glu Gly Asp Ile Ile Pro Ser Thr Gly Ile Gly Asn Thr
180 185 190
Ala Thr Phe Val Glu Asn Ala Asp Lys Val Ile Val Glu Ile Asn Glu
195 200 205
Ala Gln Pro Leu Glu Leu Glu Gly Met Ala Asp Ile Tyr Thr Leu Lys
210 215 220
Asn Pro Pro Arg Arg Glu Pro Ile Pro Ile Val Asn Ala Gly Asn Arg
225 230 235 240
Ile Gly Thr Thr Tyr Val Thr Cys Gly Ser Glu Lys Ile Cys Ala Ile
245 250 255
Val Met Thr Asn Thr Gln Asp Lys Thr Arg Pro Leu Thr Glu Val Ser
260 265 270
Pro Val Ser Gln Ala Ile Ser Asp Asn Leu Ile Gly Phe Leu Asn Lys
275 280 285
Glu Val Glu Glu Gly Lys Leu Pro Lys Asn Leu Leu Pro Ile Gln Ser
290 295 300
Gly Val Gly Ser Val Ala Asn Ala Val Leu Ala Gly Leu Cys Glu Ser
305 310 315 320
Asn Phe Lys Asn Leu Ser Cys Tyr Thr Glu Val Ile Gln Asp Ser Met
325 330 335
Leu Lys Leu Ile Lys Cys Gly Lys Ala Asp Val Val Ser Gly Thr Ser
340 345 350
Ile Ser Pro Ser Pro Glu Met Leu Pro Glu Phe Ile Lys Asp Ile Asn
355 360 365
Phe Phe Arg Glu Lys Ile Val Leu Arg Pro Gln Glu Ile Ser Asn Asn
370 375 380
Pro Glu Ile Ala Arg Arg Ile Gly Val Ile Ser Ile Asn Thr Ala Leu
385 390 395 400
Glu Val Asp Ile Tyr Gly Asn Val Asn Ser Thr His Val Met Gly Ser
405 410 415
Lys Met Met Asn Gly Ile Gly Gly Ser Gly Asp Phe Ala Arg Asn Ala
420 425 430
Tyr Leu Thr Ile Phe Thr Thr Glu Ser Ile Ala Lys Lys Gly Asp Ile
435 440 445
Ser Ser Ile Val Pro Met Val Ser His Val Asp His Thr Glu His Asp
450 455 460
Val Met Val Ile Val Thr Glu Gln Gly Val Ala Asp Leu Arg Gly Leu
465 470 475 480
Ser Pro Arg Glu Lys Ala Val Ala Ile Ile Glu Asn Cys Val His Pro
485 490 495
Asp Tyr Lys Asp Met Leu Met Glu Tyr Phe Glu Glu Ala Cys Lys Ser
500 505 510
Ser Gly Gly Asn Thr Pro His Asn Leu Glu Lys Ala Leu Ser Trp His
515 520 525
Thr Lys Phe Ile Lys Thr Gly Ser Met Lys
530 535
<210> 125
<211> 232
<212> PRT
<213> Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) 3-oxoacid CoA-transferase subunit A family enzyme (Q4KEA3)
<400> 125
Met Ala Gly Phe Asp Lys Arg Val Ala Ser Tyr Glu Glu Ala Leu Glu
1 5 10 15
Gly Leu Gln Asp Gly Met Thr Val Ile Ala Gly Gly Phe Gly Leu Cys
20 25 30
Gly Ile Pro Glu Asn Leu Ile Ala Glu Ile Lys Arg Arg Gly Thr Arg
35 40 45
Asp Leu Thr Val Val Ser Asn Asn Cys Gly Val Asp Gly Phe Gly Leu
50 55 60
Gly Val Leu Leu Glu Asp Arg Gln Ile Ser Lys Val Ile Ala Ser Tyr
65 70 75 80
Val Gly Glu Asn Ala Leu Phe Glu Lys Gln Leu Leu Ser Gly Glu Ile
85 90 95
Glu Val Val Leu Thr Pro Gln Gly Thr Leu Ala Glu Lys Met Arg Ala
100 105 110
Gly Gly Ala Gly Ile Pro Ala Phe Phe Thr Ala Thr Gly Val Gly Thr
115 120 125
Pro Val Ala Asp Gly Lys Glu Thr Arg Glu Phe Lys Gly Arg Thr Tyr
130 135 140
Leu Met Glu Glu Ser Ile Thr Gly Asp Phe Ala Ile Val Lys Gly Trp
145 150 155 160
Lys Ala Asp His Phe Gly Asn Val Val Tyr Arg His Thr Ala Gln Asn
165 170 175
Phe Asn Pro Leu Ala Ala Thr Ala Gly Lys Ile Thr Val Val Glu Val
180 185 190
Glu Glu Ile Val Glu Pro Gly Glu Leu Asp Pro Thr Gln Ile His Thr
195 200 205
Pro Gly Ile Tyr Val Asp Arg Val Ile Cys Gly Thr Phe Glu Lys Arg
210 215 220
Ile Glu Gln Arg Thr Val Arg Lys
225 230
<210> 126
<211> 219
<212> PRT
<213> Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) 3-oxoacid CoA-transferase subunit B family enzyme (Q4KEA4)
<400> 126
Met Ala Leu Thr Arg Glu Gln Met Ala Gln Arg Val Ala Arg Glu Leu
1 5 10 15
Gln Asp Gly Phe Tyr Val Asn Leu Gly Ile Gly Ile Pro Thr Leu Val
20 25 30
Ala Asn Tyr Ile Pro Asp Gly Met Glu Val Met Leu Gln Ser Glu Asn
35 40 45
Gly Leu Leu Gly Met Gly Ala Phe Pro Thr Glu Asp Glu Val Asp Ala
50 55 60
Asp Met Ile Asn Ala Gly Lys Gln Thr Val Thr Ala Arg Ile Gly Ala
65 70 75 80
Ser Ile Phe Ser Ser Ala Glu Ser Phe Ala Met Ile Arg Gly Gly His
85 90 95
Ile Asp Leu Thr Val Leu Gly Ala Phe Glu Val Asp Val Gln Gly Asn
100 105 110
Ile Ala Ser Trp Met Ile Pro Gly Lys Leu Val Lys Gly Met Gly Gly
115 120 125
Ala Met Asp Leu Val Ala Gly Ala Glu Asn Ile Ile Val Thr Met Thr
130 135 140
His Ala Ser Lys Asp Gly Glu Ser Lys Leu Leu Ser Arg Cys Ser Leu
145 150 155 160
Pro Leu Thr Gly Ala Gln Cys Ile Lys Arg Val Leu Thr Asp Leu Ala
165 170 175
Tyr Leu Glu Ile Glu Asn Gly Ala Phe Ile Leu Lys Glu Arg Ala Pro
180 185 190
Gly Val Ser Val Glu Glu Ile Val Ser Lys Thr Ala Gly Lys Leu Ile
195 200 205
Val Pro Asp His Val Pro Glu Met Gln Phe Ala
210 215
<210> 127
<211> 477
<212> PRT
<213> Acinetobacter sp. NCIMB9871 6-oxohexanoate dehydrogenase (Q9R2F4)
<400> 127
Met Asn Tyr Pro Asn Ile Pro Leu Tyr Ile Asn Gly Glu Phe Leu Asp
1 5 10 15
His Thr Asn Arg Asp Val Lys Glu Val Phe Asn Pro Val Asn His Glu
20 25 30
Cys Ile Gly Leu Met Ala Cys Ala Ser Gln Ala Asp Leu Asp Tyr Ala
35 40 45
Leu Glu Ser Ser Gln Gln Ala Phe Leu Arg Trp Lys Lys Thr Ser Pro
50 55 60
Ile Thr Arg Ser Glu Ile Leu Arg Thr Phe Ala Lys Leu Ala Arg Glu
65 70 75 80
Lys Ala Ala Glu Ile Gly Arg Asn Ile Thr Leu Asp Gln Gly Lys Pro
85 90 95
Leu Lys Glu Ala Ile Ala Glu Val Thr Val Cys Ala Glu His Ala Glu
100 105 110
Trp His Ala Glu Glu Cys Arg Arg Ile Tyr Gly Arg Val Ile Pro Pro
115 120 125
Arg Asn Pro Asn Val Gln Gln Leu Val Val Arg Glu Pro Leu Gly Val
130 135 140
Cys Leu Ala Phe Ser Pro Trp Asn Phe Pro Phe Asn Gln Ala Ile Arg
145 150 155 160
Lys Ile Ser Ala Ala Ile Ala Ala Gly Cys Thr Ile Ile Val Lys Gly
165 170 175
Ser Gly Asp Thr Pro Ser Ala Val Tyr Ala Ile Ala Gln Leu Phe His
180 185 190
Glu Ala Gly Leu Pro Asn Gly Val Leu Asn Val Ile Trp Gly Asp Ser
195 200 205
Asn Phe Ile Ser Asp Tyr Met Ile Lys Ser Pro Ile Ile Gln Lys Ile
210 215 220
Ser Phe Thr Gly Ser Thr Pro Val Gly Lys Lys Leu Ala Ser Gln Ala
225 230 235 240
Ser Leu Tyr Met Lys Pro Cys Thr Met Glu Leu Gly Gly His Ala Pro
245 250 255
Val Ile Val Cys Asp Asp Ala Asp Ile Asp Ala Ala Val Glu His Leu
260 265 270
Val Gly Tyr Lys Phe Arg Asn Ala Gly Gln Val Cys Val Ser Pro Thr
275 280 285
Arg Phe Tyr Val Gln Glu Gly Ile Tyr Lys Glu Phe Ser Glu Lys Val
290 295 300
Val Leu Arg Ala Lys Gln Ile Lys Val Gly Cys Gly Leu Asp Ala Ser
305 310 315 320
Ser Asp Met Gly Pro Leu Ala Gln Ala Arg Arg Met His Ala Met Gln
325 330 335
Gln Ile Val Glu Asp Ala Val His Lys Gly Ser Lys Leu Leu Leu Gly
340 345 350
Gly Asn Lys Ile Ser Asp Lys Gly Asn Phe Phe Glu Pro Thr Val Leu
355 360 365
Gly Asp Leu Cys Asn Asp Thr Gln Phe Met Asn Asp Glu Pro Phe Gly
370 375 380
Pro Ile Ile Gly Leu Ile Pro Phe Asp Thr Ile Asp His Val Leu Glu
385 390 395 400
Glu Ala Asn Arg Leu Pro Phe Gly Leu Ala Ser Tyr Ala Phe Thr Thr
405 410 415
Ser Ser Lys Asn Ala His Gln Ile Ser Tyr Gly Leu Glu Ala Gly Met
420 425 430
Val Ser Ile Asn His Met Gly Leu Ala Leu Ala Glu Thr Pro Phe Gly
435 440 445
Gly Ile Lys Asp Ser Gly Phe Gly Ser Glu Gly Gly Ile Glu Thr Phe
450 455 460
Asp Gly Tyr Leu Arg Thr Lys Phe Ile Thr Gln Leu Asn
465 470 475
<210> 128
<211> 473
<212> PRT
<213> Brucella melitensis 6-oxohexanoate dehydrogenase (Q8YDF1)
<400> 128
Met Arg Ile Gly Lys Met Glu Met Gln Thr Arg Tyr Pro Asp Val Lys
1 5 10 15
Leu Phe Ile Asp Gly Thr Trp Arg Asp Gly Ser Arg Gly Glu Thr Ile
20 25 30
Glu Ile Phe Asn Pro Ala Thr Asp Glu Val Ile Gly His Ile Ala Arg
35 40 45
Ala Thr Thr Ala Asp Leu Asp Asp Ala Leu Ala Ala Val Asp Arg Gly
50 55 60
Phe Glu Ala Trp Ser Lys Val Ser Ala Phe Asp Arg Tyr Lys Ile Met
65 70 75 80
Arg Arg Ala Ala Asp Ile Phe Arg Ser Arg Gly Glu Glu Val Ala Arg
85 90 95
Leu Leu Thr Met Glu Gln Gly Lys Pro Leu Ala Glu Ala Arg Ile Glu
100 105 110
Ala Ala Ala Ala Cys Asp Leu Ile Asp Trp Phe Ala Glu Glu Ala Arg
115 120 125
Arg Ser Tyr Gly Arg Ile Val Pro Pro Arg Gln Ala Tyr Val Met Gln
130 135 140
Ala Glu Val Lys Glu Pro Val Gly Pro Val Ala Ala Phe Thr Pro Trp
145 150 155 160
Asn Phe Pro Ile Asn Gln Ala Val Arg Lys Ile Ser Ala Ala Leu Ala
165 170 175
Ala Gly Cys Ser Ile Leu Leu Lys Ala Ala Glu Asp Thr Pro Ala Ala
180 185 190
Pro Ala Glu Leu Val Arg Ala Phe Ala Glu Ala Gly Leu Pro Asp Gly
195 200 205
Ala Ile Asn Leu Val Tyr Gly Asp Pro Ala Glu Ile Ser Ala Tyr Leu
210 215 220
Ile Pro His Pro Val Ile Arg Lys Val Ser Phe Thr Gly Ser Thr Gln
225 230 235 240
Val Gly Lys Gln Leu Ala Ala Leu Ala Gly Leu His Met Lys Arg Val
245 250 255
Thr Met Glu Leu Gly Gly His Ala Pro Val Ile Ile Ala Ala Asp Ala
260 265 270
Asp Val Glu Gln Ala Ile Lys Val Val Ser Gly Ser Lys Phe Arg Asn
275 280 285
Ala Gly Gln Val Cys Ile Ser Pro Thr Arg Phe Leu Ile Glu Asn Ser
290 295 300
Val Tyr Asp Gln Val Val Glu Gly Met Ala Ala Tyr Ala Thr Ser Leu
305 310 315 320
Lys Val Gly Asp Gly Leu Glu Ala Gly Thr Thr Met Gly Pro Leu Val
325 330 335
Asn Ala Lys Arg Val Asn Ala Met Glu Arg Leu Val Gln Asp Ala Arg
340 345 350
Glu His Lys Ala Arg Val Val Thr Gly Gly Glu Arg Ile Gly Asn Arg
355 360 365
Gly Asn Phe Phe Glu Pro Thr Ile Leu Ala Asp Val Pro Arg Asp Ala
370 375 380
Ala Ile Met Asn Glu Glu Pro Phe Gly Pro Val Ala Leu Leu Asn Arg
385 390 395 400
Phe Asp Ala Leu Asp Glu Ala Leu Ser Glu Ala Asn Arg Leu Asn Tyr
405 410 415
Gly Leu Ala Ala Tyr Ala Phe Thr Gly Ser Ser Ala Lys Ala Ala Arg
420 425 430
Ile Ser Ser Thr Val Arg Ser Gly Met Ile Thr Ile Asn Gln Leu Arg
435 440 445
Ser Gly Pro Ala Gly Ser Ala Leu Arg Arg Asp Gln Arg Phe Arg Leu
450 455 460
Trp Asn Gly Arg Arg Cys Arg Arg Ala
465 470
<210> 129
<211> 1371
<212> DNA
<213> Artificial
<220>
<223> Pseudomonas aeruginosa (gi 9946143)
<400> 129
atgaacagcc aaatcaccaa cgccaagacc cgtgagtggc aggcgttgag ccgcgaccac 60
catctgccgc cgttcaccga ctacaagcag ttgaacgaga agggcgcgcg gatcatcacc 120
aaggccgaag gcgtctatat ctgggacagc gagggcaaca agatcctcga tgcgatggcc 180
ggcctctggt gcgtcaacgt cggctacggc cgcgaggagc tggtccaggc cgccacccgg 240
cagatgcgcg agttgccgtt ctacaacctg ttcttccaga ccgcccaccc gccggtggtc 300
gagctggcca aggcgatcgc cgacgtcgct ccggaaggca tgaaccacgt gttcttcacc 360
ggctccggct ccgaggccaa cgacaccgtg ctgcgtatgg tccgccacta ttgggcgacc 420
aagggccagc cgcagaagaa agtggtgatc ggccgctgga acggctacca cggctccacc 480
gtcgccggcg tcagcctggg cggcatgaag gcgttgcatg agcagggtga tttccccatc 540
ccgggcatcg tccacatcgc ccagccctac tggtacggcg agggcggcga catgtcgccg 600
gacgagttcg gcgtctgggc cgccgagcag ttggagaaga agattctcga agtgggcgag 660
gaaaacgtcg ccgccttcat cgccgagccg atccagggcg ccggcggcgt gatcgtcccg 720
ccggacacct actggccgaa gatccgcgag atcctcgcca agtacgacat cctgttcatc 780
gccgacgaag tgatctgcgg cttcggccgt accggcgagt ggttcggcag ccagtactac 840
ggcaacgccc cggacctgat gccgatcgcc aagggcctca cctccggcta catccccatg 900
ggcggggtgg tggtgcgcga cgagatcgtc gaagtgctca accagggcgg cgagttctac 960
cacggcttca cctattccgg tcacccggtg gcggccgccg tggccctgga gaacatccgc 1020
atcctgcgcg aagagaagat catcgagaag gtgaaggcgg aaacggcacc gtatttgcag 1080
aaacgctggc aggagctggc cgaccacccg ttggtgggcg aagcgcgcgg ggtcggcatg 1140
gtcgccgccc tggagctggt caagaacaag aagacccgcg agcgtttcac cgacaagggc 1200
gtcgggatgc tgtgccggga acattgtttc cgcaacggtt tgatcatgcg cgcggtgggc 1260
gacactatga ttatctcgcc gccgctggtg atcgatccgt cgcagatcga tgagttgatc 1320
accctggcgc gcaagtgcct cgatcagacc gccgccgccg tcctggcttg a 1371
<210> 130
<211> 456
<212> PRT
<213> Pseudomonas aeruginosa (gi 9946143)
<400> 130
Met Asn Ser Gln Ile Thr Asn Ala Lys Thr Arg Glu Trp Gln Ala Leu
1 5 10 15
Ser Arg Asp His His Leu Pro Pro Phe Thr Asp Tyr Lys Gln Leu Asn
20 25 30
Glu Lys Gly Ala Arg Ile Ile Thr Lys Ala Glu Gly Val Tyr Ile Trp
35 40 45
Asp Ser Glu Gly Asn Lys Ile Leu Asp Ala Met Ala Gly Leu Trp Cys
50 55 60
Val Asn Val Gly Tyr Gly Arg Glu Glu Leu Val Gln Ala Ala Thr Arg
65 70 75 80
Gln Met Arg Glu Leu Pro Phe Tyr Asn Leu Phe Phe Gln Thr Ala His
85 90 95
Pro Pro Val Val Glu Leu Ala Lys Ala Ile Ala Asp Val Ala Pro Glu
100 105 110
Gly Met Asn His Val Phe Phe Thr Gly Ser Gly Ser Glu Ala Asn Asp
115 120 125
Thr Val Leu Arg Met Val Arg His Tyr Trp Ala Thr Lys Gly Gln Pro
130 135 140
Gln Lys Lys Val Val Ile Gly Arg Trp Asn Gly Tyr His Gly Ser Thr
145 150 155 160
Val Ala Gly Val Ser Leu Gly Gly Met Lys Ala Leu His Glu Gln Gly
165 170 175
Asp Phe Pro Ile Pro Gly Ile Val His Ile Ala Gln Pro Tyr Trp Tyr
180 185 190
Gly Glu Gly Gly Asp Met Ser Pro Asp Glu Phe Gly Val Trp Ala Ala
195 200 205
Glu Gln Leu Glu Lys Lys Ile Leu Glu Val Gly Glu Glu Asn Val Ala
210 215 220
Ala Phe Ile Ala Glu Pro Ile Gln Gly Ala Gly Gly Val Ile Val Pro
225 230 235 240
Pro Asp Thr Tyr Trp Pro Lys Ile Arg Glu Ile Leu Ala Lys Tyr Asp
245 250 255
Ile Leu Phe Ile Ala Asp Glu Val Ile Cys Gly Phe Gly Arg Thr Gly
260 265 270
Glu Trp Phe Gly Ser Gln Tyr Tyr Gly Asn Ala Pro Asp Leu Met Pro
275 280 285
Ile Ala Lys Gly Leu Thr Ser Gly Tyr Ile Pro Met Gly Gly Val Val
290 295 300
Val Arg Asp Glu Ile Val Glu Val Leu Asn Gln Gly Gly Glu Phe Tyr
305 310 315 320
His Gly Phe Thr Tyr Ser Gly His Pro Val Ala Ala Ala Val Ala Leu
325 330 335
Glu Asn Ile Arg Ile Leu Arg Glu Glu Lys Ile Ile Glu Lys Val Lys
340 345 350
Ala Glu Thr Ala Pro Tyr Leu Gln Lys Arg Trp Gln Glu Leu Ala Asp
355 360 365
His Pro Leu Val Gly Glu Ala Arg Gly Val Gly Met Val Ala Ala Leu
370 375 380
Glu Leu Val Lys Asn Lys Lys Thr Arg Glu Arg Phe Thr Asp Lys Gly
385 390 395 400
Val Gly Met Leu Cys Arg Glu His Cys Phe Arg Asn Gly Leu Ile Met
405 410 415
Arg Ala Val Gly Asp Thr Met Ile Ile Ser Pro Pro Leu Val Ile Asp
420 425 430
Pro Ser Gln Ile Asp Glu Leu Ile Thr Leu Ala Arg Lys Cys Leu Asp
435 440 445
Gln Thr Ala Ala Ala Val Leu Ala
450 455
<210> 131
<211> 70
<212> DNA
<213> Artificial
<220>
<223> primer
<400> 131
ggggacaagt ttgtacaaaa aagcaggcta ggaggaatta accatgaaca gccaaatcac 60
caacgccaag 70
<210> 132
<211> 49
<212> DNA
<213> Artificial
<220>
<223> primer
<400> 132
ggggaccact ttgtacaaga aagctgggtt caagccagga cggcggcgg 49
<210> 133
<211> 1353
<212> DNA
<213> Artificial
<220>
<223> Bacillus subtilis aminotransferase gene (gi16077991)
<400> 133
atggagatga tggggatgga aaacattcag caaaatcagg gattaaagca aaaagatgag 60
caatttgtgt ggcatgccat gaagggagcg catcaagcgg acagcctgat agcccagaag 120
gccgaagggg cctgggtaac cgacacagac ggacgccgct atttggatgc gatgtccggt 180
ttgtggtgcg tcaacattgg ttacggcaga aaggagcttg cggaggctgc ctatgagcaa 240
ctaaaggagc tgccttacta cccgttaacg caaagtcacg cacccgcaat tcaactggcg 300
gaaaagctga atgaatggct tggcggcgat tatgttattt ttttttccaa cagcggatcg 360
gaagcaaacg aaactgcttt taaaattgcc cgccagtacc atctgcaaaa cggcgaccac 420
agccgttata aattcatctc aagatatcgg gcataccacg gcaatacatt gggagcgctc 480
tcagctaccg gacaggcgca gcggaaatat aaatacgagc ctttgagcca agggttcctg 540
catgcagctc cgccagatat ataccggaat cctgatgatg cagacacgct tgaaagcgca 600
aatgaaatcg accgcatcat gacatgggaa ttaagcgaaa cgattgccgg ggtcattatg 660
gagcccatca ttacaggcgg aggcatccta atgccgccgg acggatatat gaagaaggtg 720
gaggacattt gccggcgcca cggagccctt ttgatttgcg atgaagtgat ctgcgggttt 780
ggacggacag gtgagccgtt cgggtttatg cactacggtg tgaagcctga tatcattacg 840
atggcaaagg gaatcacaag cgcgtatctg ccattgtcag cgactgctgt gaaacgggac 900
attttcgaag cgtatcaggg ggaagctcct tatgaccgtt tccgccacgt gaacacgttc 960
ggcggaagcc cggctgcctg tgctttggcg ttgaaaaacc tgcaaattat ggaggacgaa 1020
cagctgattc agcgatcccg tgatcttgga gcaaagcttt taggtgagct tcaagctctg 1080
agagaacacc cggcagtcgg ggatgttaga ggaaaagggc tgctgatcgg aatcgaactc 1140
gtcaaagaca aattgactaa agagccggct gatgccgcca aagtaaacca agtggttgcg 1200
gcgtgcaaag aaaaagggct gatcatcggc aaaaacggcg atacagtcgc cggctacaac 1260
aatgtcatcc acgttgcgcc gccattttgc ctgacagaag aggacctttc ctttatcgtg 1320
aaaacggtga aagaaagctt tcaaacgata taa 1353
<210> 134
<211> 450
<212> PRT
<213> amino acid sequence of B. subtilis aminotransferase (gi16077991)
<400> 134
Met Glu Met Met Gly Met Glu Asn Ile Gln Gln Asn Gln Gly Leu Lys
1 5 10 15
Gln Lys Asp Glu Gln Phe Val Trp His Ala Met Lys Gly Ala His Gln
20 25 30
Ala Asp Ser Leu Ile Ala Gln Lys Ala Glu Gly Ala Trp Val Thr Asp
35 40 45
Thr Asp Gly Arg Arg Tyr Leu Asp Ala Met Ser Gly Leu Trp Cys Val
50 55 60
Asn Ile Gly Tyr Gly Arg Lys Glu Leu Ala Glu Ala Ala Tyr Glu Gln
65 70 75 80
Leu Lys Glu Leu Pro Tyr Tyr Pro Leu Thr Gln Ser His Ala Pro Ala
85 90 95
Ile Gln Leu Ala Glu Lys Leu Asn Glu Trp Leu Gly Gly Asp Tyr Val
100 105 110
Ile Phe Phe Ser Asn Ser Gly Ser Glu Ala Asn Glu Thr Ala Phe Lys
115 120 125
Ile Ala Arg Gln Tyr His Leu Gln Asn Gly Asp His Ser Arg Tyr Lys
130 135 140
Phe Ile Ser Arg Tyr Arg Ala Tyr His Gly Asn Thr Leu Gly Ala Leu
145 150 155 160
Ser Ala Thr Gly Gln Ala Gln Arg Lys Tyr Lys Tyr Glu Pro Leu Ser
165 170 175
Gln Gly Phe Leu His Ala Ala Pro Pro Asp Ile Tyr Arg Asn Pro Asp
180 185 190
Asp Ala Asp Thr Leu Glu Ser Ala Asn Glu Ile Asp Arg Ile Met Thr
195 200 205
Trp Glu Leu Ser Glu Thr Ile Ala Gly Val Ile Met Glu Pro Ile Ile
210 215 220
Thr Gly Gly Gly Ile Leu Met Pro Pro Asp Gly Tyr Met Lys Lys Val
225 230 235 240
Glu Asp Ile Cys Arg Arg His Gly Ala Leu Leu Ile Cys Asp Glu Val
245 250 255
Ile Cys Gly Phe Gly Arg Thr Gly Glu Pro Phe Gly Phe Met His Tyr
260 265 270
Gly Val Lys Pro Asp Ile Ile Thr Met Ala Lys Gly Ile Thr Ser Ala
275 280 285
Tyr Leu Pro Leu Ser Ala Thr Ala Val Lys Arg Asp Ile Phe Glu Ala
290 295 300
Tyr Gln Gly Glu Ala Pro Tyr Asp Arg Phe Arg His Val Asn Thr Phe
305 310 315 320
Gly Gly Ser Pro Ala Ala Cys Ala Leu Ala Leu Lys Asn Leu Gln Ile
325 330 335
Met Glu Asp Glu Gln Leu Ile Gln Arg Ser Arg Asp Leu Gly Ala Lys
340 345 350
Leu Leu Gly Glu Leu Gln Ala Leu Arg Glu His Pro Ala Val Gly Asp
355 360 365
Val Arg Gly Lys Gly Leu Leu Ile Gly Ile Glu Leu Val Lys Asp Lys
370 375 380
Leu Thr Lys Glu Pro Ala Asp Ala Ala Lys Val Asn Gln Val Val Ala
385 390 395 400
Ala Cys Lys Glu Lys Gly Leu Ile Ile Gly Lys Asn Gly Asp Thr Val
405 410 415
Ala Gly Tyr Asn Asn Val Ile His Val Ala Pro Pro Phe Cys Leu Thr
420 425 430
Glu Glu Asp Leu Ser Phe Ile Val Lys Thr Val Lys Glu Ser Phe Gln
435 440 445
Thr Ile
450
<210> 135
<211> 1407
<212> DNA
<213> Artificial
<220>
<223> Pseudomonas aeruginosa aminotransferase gene (seq ID gi9951072)
<400> 135
atgaacgcaa gactgcacgc cacgtccccc ctcggcgacg ccgacctggt ccgtgccgac 60
caggcccact acatgcacgg ctaccacgtg ttcgacgacc accgcgtcaa cggctcgctg 120
aacatcgccg ccggcgacgg cgcctatatc tacgacaccg ccggcaaccg ctacctcgac 180
gcggtgggcg gcatgtggtg caccaacatc ggcctggggc gcgaggaaat ggctcgcacc 240
gtggccgagc agacccgcct gctggcctat tccaatccct tctgcgacat ggccaacccg 300
cgcgccatcg aactctgccg caagctcgcc gagctggccc ccggcgacct cgaccacgtg 360
ttcctcacca ccggcggttc caccgccgtg gacaccgcga tccgcctcat gcactactac 420
cagaactgcc gcggcaagcg cgccaagaag cacgtcatca cgcggatcaa cgcctaccac 480
ggctcgacct tcctcggcat gtcgctgggc ggcaagagcg ccgaccggcc ggccgagttc 540
gacttcctcg acgagcgcat ccaccacctc gcctgtccct attactaccg cgctccggaa 600
gggctgggcg aagccgagtt cctcgatggc ctggtggacg agttcgaacg caagatcctc 660
gaactgggcg ccgaccgggt gggggcgttc atctccgagc cggtgttcgg ctccggcggc 720
gtgatcgtcc cgcccgcggg ctaccacagg cggatgtggg agctgtgcca gcgctacgac 780
gtgctgtaca tctccgacga agtggtgacc tccttcggcc gcctcggcca cttcttcgcc 840
agccaggcgg tgttcggcgt acagccggac atcatcctca ccgccaaggg cctcacctcc 900
ggctaccagc cgctgggcgc gtgcatcttc tcccggcgca tctgggaggt gatcgccgag 960
ccggacaagg gccgctgctt cagccatggt ttcacctact ccggccaccc ggtggcctgc 1020
gcggcggcgc tgaagaacat cgagatcatc gagcgcgagg gcttgctcgc ccacgccgac 1080
gaggtcggcc gctacttcga ggagcgcctg caaagcctcc gcgacctgcc catcgtcggc 1140
gacgtgcgcg ggatgcgctt catggcctgt gtcgagttcg tcgccgacaa ggcgagcaag 1200
gcgctgtttc cggaaagcct gaacatcggc gagtgggtcc acctgcgggc gcagaagcgc 1260
ggcctgctgg ttcgtccgat cgtccacctg aacgtgatgt cgccgccgct gatcctcacc 1320
cgcgaacagg tcgataccgt ggtccgggtg ctgcgcgaga gcatcgagga aaccgtggag 1380
gatcttgtcc gcgccggtca ccggtaa 1407
<210> 136
<211> 468
<212> PRT
<213> amino acid sequence of Pseudomonas aeruginosa aminotransferase (seqID gi9951072)
<400> 136
Met Asn Ala Arg Leu His Ala Thr Ser Pro Leu Gly Asp Ala Asp Leu
1 5 10 15
Val Arg Ala Asp Gln Ala His Tyr Met His Gly Tyr His Val Phe Asp
20 25 30
Asp His Arg Val Asn Gly Ser Leu Asn Ile Ala Ala Gly Asp Gly Ala
35 40 45
Tyr Ile Tyr Asp Thr Ala Gly Asn Arg Tyr Leu Asp Ala Val Gly Gly
50 55 60
Met Trp Cys Thr Asn Ile Gly Leu Gly Arg Glu Glu Met Ala Arg Thr
65 70 75 80
Val Ala Glu Gln Thr Arg Leu Leu Ala Tyr Ser Asn Pro Phe Cys Asp
85 90 95
Met Ala Asn Pro Arg Ala Ile Glu Leu Cys Arg Lys Leu Ala Glu Leu
100 105 110
Ala Pro Gly Asp Leu Asp His Val Phe Leu Thr Thr Gly Gly Ser Thr
115 120 125
Ala Val Asp Thr Ala Ile Arg Leu Met His Tyr Tyr Gln Asn Cys Arg
130 135 140
Gly Lys Arg Ala Lys Lys His Val Ile Thr Arg Ile Asn Ala Tyr His
145 150 155 160
Gly Ser Thr Phe Leu Gly Met Ser Leu Gly Gly Lys Ser Ala Asp Arg
165 170 175
Pro Ala Glu Phe Asp Phe Leu Asp Glu Arg Ile His His Leu Ala Cys
180 185 190
Pro Tyr Tyr Tyr Arg Ala Pro Glu Gly Leu Gly Glu Ala Glu Phe Leu
195 200 205
Asp Gly Leu Val Asp Glu Phe Glu Arg Lys Ile Leu Glu Leu Gly Ala
210 215 220
Asp Arg Val Gly Ala Phe Ile Ser Glu Pro Val Phe Gly Ser Gly Gly
225 230 235 240
Val Ile Val Pro Pro Ala Gly Tyr His Arg Arg Met Trp Glu Leu Cys
245 250 255
Gln Arg Tyr Asp Val Leu Tyr Ile Ser Asp Glu Val Val Thr Ser Phe
260 265 270
Gly Arg Leu Gly His Phe Phe Ala Ser Gln Ala Val Phe Gly Val Gln
275 280 285
Pro Asp Ile Ile Leu Thr Ala Lys Gly Leu Thr Ser Gly Tyr Gln Pro
290 295 300
Leu Gly Ala Cys Ile Phe Ser Arg Arg Ile Trp Glu Val Ile Ala Glu
305 310 315 320
Pro Asp Lys Gly Arg Cys Phe Ser His Gly Phe Thr Tyr Ser Gly His
325 330 335
Pro Val Ala Cys Ala Ala Ala Leu Lys Asn Ile Glu Ile Ile Glu Arg
340 345 350
Glu Gly Leu Leu Ala His Ala Asp Glu Val Gly Arg Tyr Phe Glu Glu
355 360 365
Arg Leu Gln Ser Leu Arg Asp Leu Pro Ile Val Gly Asp Val Arg Gly
370 375 380
Met Arg Phe Met Ala Cys Val Glu Phe Val Ala Asp Lys Ala Ser Lys
385 390 395 400
Ala Leu Phe Pro Glu Ser Leu Asn Ile Gly Glu Trp Val His Leu Arg
405 410 415
Ala Gln Lys Arg Gly Leu Leu Val Arg Pro Ile Val His Leu Asn Val
420 425 430
Met Ser Pro Pro Leu Ile Leu Thr Arg Glu Gln Val Asp Thr Val Val
435 440 445
Arg Val Leu Arg Glu Ser Ile Glu Glu Thr Val Glu Asp Leu Val Arg
450 455 460
Ala Gly His Arg
465
<210> 137
<211> 1335
<212> DNA
<213> Artificial
<220>
<223> Pseudomonas aeruginosa aminotransferase gene (seq ID gi9951630)
<400> 137
atgacaatga atgacgagcc gcagtcgagc agcctcgaca acttctggat gcccttcacc 60
gccaaccgcc agttcaaggc gcggccgcgc ctgctggaaa gcgccgaagg catccactat 120
atcgcccagg gcgggcgccg catcctcgac ggcaccgccg gcctctggtg ctgcaatgcc 180
ggccacggcc ggcgcgagat cagcgaagcg gtggcccggc agatcgccac cctcgactac 240
gccccgccgt tccagatggg tcacccgctg ccgttcgaac tcgccgcgcg gctgacggaa 300
atcgccccgc cgagcctgaa caaagtattc ttcaccaact ccggctcgga atcggcggac 360
accgcgctga agatcgccct tgcctaccag cgcgccatcg gccagggcac ccgcacccgc 420
ctgatcggcc gcgaactggg ctaccacggg gtcggcttcg gcggcctgtc ggtaggcggt 480
atggtcaaca accgcaaggc cttctccgcc aacctgctgc cgggggtcga ccacctgccg 540
cacaccctgg acgtcgcccg caacgccttc accgtcggcc tgcccgagca tggcgtggaa 600
aaggccgagg agctggaacg cctggtgacc ctgcacggcg ccgagaatat cgccgcggtg 660
atcgtcgagc cgatgtccgg ctcggccggc gtggtgctgc cgcccaaggg ctaccttcag 720
cggctgcgcg agataacccg caagcatggc atcctgctga tcttcgacga agtgatcacc 780
ggtttcggcc gcgtcggcga agccttcgcc gcgcagcgct ggggcgtcgt cccggacctg 840
ctgacctgcg ccaaggggct gaccaacggc agcatcccga tgggcgccgt attcgtcgac 900
gagaagatcc atgctgcctt catgcaaggc ccgcagggcg ccatcgagtt cttccacggc 960
tatacctatt ccggccatcc ggtagcctgc gccgccgccc tggcgaccct ggacatctac 1020
cgtcgcgacg acctgttcca gcgggccgtc gaactggaag gctactggca ggacgcgctg 1080
ttcagcctgc gcgacctgcc caacgtggtc gacatccgcg ccgtaggcct ggtcggcggc 1140
gtgcaactgg cgccgcacgc ggacggcccc ggcaagcgcg gctacgacgt cttcgagcgc 1200
tgcttctggg agcacgacct gatggtccgg gtgaccggcg acatcatcgc catgtcgccg 1260
ccgctgatca tcgacaagcc ccacatcgac cagatcgtcg agcgcctggc ccaggccatc 1320
cgcgccagcg tctga 1335
<210> 138
<211> 444
<212> PRT
<213> amino acid sequence of Pseudomonas aeruginosa aminotransferase (seqID gi9951630)
<400> 138
Met Thr Met Asn Asp Glu Pro Gln Ser Ser Ser Leu Asp Asn Phe Trp
1 5 10 15
Met Pro Phe Thr Ala Asn Arg Gln Phe Lys Ala Arg Pro Arg Leu Leu
20 25 30
Glu Ser Ala Glu Gly Ile His Tyr Ile Ala Gln Gly Gly Arg Arg Ile
35 40 45
Leu Asp Gly Thr Ala Gly Leu Trp Cys Cys Asn Ala Gly His Gly Arg
50 55 60
Arg Glu Ile Ser Glu Ala Val Ala Arg Gln Ile Ala Thr Leu Asp Tyr
65 70 75 80
Ala Pro Pro Phe Gln Met Gly His Pro Leu Pro Phe Glu Leu Ala Ala
85 90 95
Arg Leu Thr Glu Ile Ala Pro Pro Ser Leu Asn Lys Val Phe Phe Thr
100 105 110
Asn Ser Gly Ser Glu Ser Ala Asp Thr Ala Leu Lys Ile Ala Leu Ala
115 120 125
Tyr Gln Arg Ala Ile Gly Gln Gly Thr Arg Thr Arg Leu Ile Gly Arg
130 135 140
Glu Leu Gly Tyr His Gly Val Gly Phe Gly Gly Leu Ser Val Gly Gly
145 150 155 160
Met Val Asn Asn Arg Lys Ala Phe Ser Ala Asn Leu Leu Pro Gly Val
165 170 175
Asp His Leu Pro His Thr Leu Asp Val Ala Arg Asn Ala Phe Thr Val
180 185 190
Gly Leu Pro Glu His Gly Val Glu Lys Ala Glu Glu Leu Glu Arg Leu
195 200 205
Val Thr Leu His Gly Ala Glu Asn Ile Ala Ala Val Ile Val Glu Pro
210 215 220
Met Ser Gly Ser Ala Gly Val Val Leu Pro Pro Lys Gly Tyr Leu Gln
225 230 235 240
Arg Leu Arg Glu Ile Thr Arg Lys His Gly Ile Leu Leu Ile Phe Asp
245 250 255
Glu Val Ile Thr Gly Phe Gly Arg Val Gly Glu Ala Phe Ala Ala Gln
260 265 270
Arg Trp Gly Val Val Pro Asp Leu Leu Thr Cys Ala Lys Gly Leu Thr
275 280 285
Asn Gly Ser Ile Pro Met Gly Ala Val Phe Val Asp Glu Lys Ile His
290 295 300
Ala Ala Phe Met Gln Gly Pro Gln Gly Ala Ile Glu Phe Phe His Gly
305 310 315 320
Tyr Thr Tyr Ser Gly His Pro Val Ala Cys Ala Ala Ala Leu Ala Thr
325 330 335
Leu Asp Ile Tyr Arg Arg Asp Asp Leu Phe Gln Arg Ala Val Glu Leu
340 345 350
Glu Gly Tyr Trp Gln Asp Ala Leu Phe Ser Leu Arg Asp Leu Pro Asn
355 360 365
Val Val Asp Ile Arg Ala Val Gly Leu Val Gly Gly Val Gln Leu Ala
370 375 380
Pro His Ala Asp Gly Pro Gly Lys Arg Gly Tyr Asp Val Phe Glu Arg
385 390 395 400
Cys Phe Trp Glu His Asp Leu Met Val Arg Val Thr Gly Asp Ile Ile
405 410 415
Ala Met Ser Pro Pro Leu Ile Ile Asp Lys Pro His Ile Asp Gln Ile
420 425 430
Val Glu Arg Leu Ala Gln Ala Ile Arg Ala Ser Val
435 440
<210> 139
<211> 467
<212> PRT
<213> Escherichia coli (strain K12) Ethanolamine utilization protein eutE(P77445)
<400> 139
Met Asn Gln Gln Asp Ile Glu Gln Val Val Lys Ala Val Leu Leu Lys
1 5 10 15
Met Gln Ser Ser Asp Thr Pro Ser Ala Ala Val His Glu Met Gly Val
20 25 30
Phe Ala Ser Leu Asp Asp Ala Val Ala Ala Ala Lys Val Ala Gln Gln
35 40 45
Gly Leu Lys Ser Val Ala Met Arg Gln Leu Ala Ile Ala Ala Ile Arg
50 55 60
Glu Ala Gly Glu Lys His Ala Arg Asp Leu Ala Glu Leu Ala Val Ser
65 70 75 80
Glu Thr Gly Met Gly Arg Val Glu Asp Lys Phe Ala Lys Asn Val Ala
85 90 95
Gln Ala Arg Gly Thr Pro Gly Val Glu Cys Leu Ser Pro Gln Val Leu
100 105 110
Thr Gly Asp Asn Gly Leu Thr Leu Ile Glu Asn Ala Pro Trp Gly Val
115 120 125
Val Ala Ser Val Thr Pro Ser Thr Asn Pro Ala Ala Thr Val Ile Asn
130 135 140
Asn Ala Ile Ser Leu Ile Ala Ala Gly Asn Ser Val Ile Phe Ala Pro
145 150 155 160
His Pro Ala Ala Lys Lys Val Ser Gln Arg Ala Ile Thr Leu Leu Asn
165 170 175
Gln Ala Ile Val Ala Ala Gly Gly Pro Glu Asn Leu Leu Val Thr Val
180 185 190
Ala Asn Pro Asp Ile Glu Thr Ala Gln Arg Leu Phe Lys Phe Pro Gly
195 200 205
Ile Gly Leu Leu Val Val Thr Gly Gly Glu Ala Val Val Glu Ala Ala
210 215 220
Arg Lys His Thr Asn Lys Arg Leu Ile Ala Ala Gly Ala Gly Asn Pro
225 230 235 240
Pro Val Val Val Asp Glu Thr Ala Asp Leu Ala Arg Ala Ala Gln Ser
245 250 255
Ile Val Lys Gly Ala Ser Phe Asp Asn Asn Ile Ile Cys Ala Asp Glu
260 265 270
Lys Val Leu Ile Val Val Asp Ser Val Ala Asp Glu Leu Met Arg Leu
275 280 285
Met Glu Gly Gln His Ala Val Lys Leu Thr Ala Glu Gln Ala Gln Gln
290 295 300
Leu Gln Pro Val Leu Leu Lys Asn Ile Asp Glu Arg Gly Lys Gly Thr
305 310 315 320
Val Ser Arg Asp Trp Val Gly Arg Asp Ala Gly Lys Ile Ala Ala Ala
325 330 335
Ile Gly Leu Lys Val Pro Gln Glu Thr Arg Leu Leu Phe Val Glu Thr
340 345 350
Thr Ala Glu His Pro Phe Ala Val Thr Glu Leu Met Met Pro Val Leu
355 360 365
Pro Val Val Arg Val Ala Asn Val Ala Asp Ala Ile Ala Leu Ala Val
370 375 380
Lys Leu Glu Gly Gly Cys His His Thr Ala Ala Met His Ser Arg Asn
385 390 395 400
Ile Glu Asn Met Asn Gln Met Ala Asn Ala Ile Asp Thr Ser Ile Phe
405 410 415
Val Lys Asn Gly Pro Cys Ile Ala Gly Leu Gly Leu Gly Gly Glu Gly
420 425 430
Trp Thr Thr Met Thr Ile Thr Thr Pro Thr Gly Glu Gly Val Thr Ser
435 440 445
Ala Arg Thr Phe Val Arg Leu Arg Arg Cys Val Leu Val Asp Ala Phe
450 455 460
Arg Ile Val
465
<210> 140
<211> 532
<212> PRT
<213> Clostridium tetani Alcohol dehydrogenase (Q894U3)
<400> 140
Met Glu Lys Tyr Asp Lys Asp Leu Tyr Ser Ile Gln Gln Ala Arg Asn
1 5 10 15
Leu Ala Arg Leu Gly Lys Val Ala Ala Asn Lys Ile Ala Asn Tyr Thr
20 25 30
Glu Glu Gln Ile Asp Lys Ile Leu Arg Asn Met Val Arg Val Ala Glu
35 40 45
Glu Asn Ala Val Ser Leu Ala Gln Met Ala Val Glu Glu Thr Gly Phe
50 55 60
Gly Lys Val Glu Asp Lys Thr Tyr Lys Asn His Leu Ala Ala Thr Ile
65 70 75 80
Leu Tyr Asp Ser Ile Lys Asp Met Lys Thr Ile Gly Val Ile Ser Glu
85 90 95
Asp Lys Val Asn Lys Met Ile Glu Ile Ala Glu Pro Val Gly Leu Ile
100 105 110
Met Gly Ile Val Pro Ser Thr Asn Pro Thr Ser Thr Ala Ile Phe Lys
115 120 125
Ser Ile Ile Ala Ile Lys Ser Arg Asn Ala Ile Val Phe Ser Pro His
130 135 140
Pro Ala Ala Ala Lys Cys Thr Ile Arg Ala Val Glu Leu Met Arg Asp
145 150 155 160
Ala Ala Ile Glu Ala Gly Ala Pro Glu Asp Ile Ile Ala Ser Leu Thr
165 170 175
Asn Leu Thr Met Glu Ala Thr Asn Glu Leu Met Lys Asn Glu Asn Val
180 185 190
Ser Leu Ile Ile Ala Thr Gly Gly Pro Gly Met Val Lys Ala Ala Tyr
195 200 205
Ser Ser Gly Lys Pro Ala Ile Gly Val Gly Ala Gly Asn Ser Pro Ala
210 215 220
Tyr Ile Glu Arg Thr Ala Asn Val Glu Lys Ala Ile Arg Asp Ile Ile
225 230 235 240
Ser Ser Lys Ser Phe Asp Tyr Gly Thr Ile Cys Ala Ser Glu Gln Ser
245 250 255
Ile Ile Cys Glu Glu Cys Asn His Asp Ala Val Val Glu Glu Leu Lys
260 265 270
Lys Gln Gly Gly Tyr Phe Met Thr Ala Glu Glu Thr Glu Lys Val Cys
275 280 285
Lys Leu Leu Phe Lys Asn Gly His Thr Met Asn Ala Lys Phe Val Gly
290 295 300
Arg Ser Pro Gln Val Ile Ala Asn Ala Ala Gly Phe Thr Val Ser Glu
305 310 315 320
Asp Ile Lys Val Leu Ile Gly Lys Gln Asn Gly Val Gly Asn Gly Asn
325 330 335
Pro Leu Ser Phe Glu Lys Leu Thr Thr Val Leu Ala Phe Tyr Thr Val
340 345 350
Lys Asp Trp His Glu Ala Cys Glu Leu Ser Ile Glu Leu Leu Gln Asn
355 360 365
Gly Ile Gly His Thr Met Ser Ile His Thr Gln Asn Asp Asp Ile Val
370 375 380
Met Glu Phe Ala Lys Lys Pro Ala Ser Arg Ile Leu Val Asn Thr Gly
385 390 395 400
Gly Ser Gln Gly Gly Thr Gly Ala Ser Thr Gly Leu Lys Pro Ala Phe
405 410 415
Thr Leu Gly Cys Gly Thr Trp Gly Gly Ser Ser Val Ser Glu Asn Val
420 425 430
Thr Pro Glu His Leu Ile Asn Lys Lys Arg Val Ala Tyr Gly Leu Lys
435 440 445
Asp Cys Ala Thr Leu Val Gln Asn Asp Pro Thr Phe Asn Cys Ile Lys
450 455 460
Thr Ala Ser Asn Cys Arg Gly Val Lys Asn Gln Phe Met Asn Met Ser
465 470 475 480
Pro Ala Gln Ile Ala Ala Ala Ala Glu Val Leu Asn Lys Cys Asn Asp
485 490 495
Tyr Ala Lys Asn Thr Gly Cys Ser Asn Glu Ser Lys Glu Lys Asp Ala
500 505 510
Asn Asn Glu Glu Leu Leu Ser Leu Val Asn Gln Ile Val Ala Ala Met
515 520 525
Lys Gly Ala Asn
530
<210> 141
<211> 297
<212> PRT
<213> Burkholderia thailandensis (strain E264 / ATCC 700388 )Acetaldehyde dehydrogenase (Q2T7T0)
<400> 141
Met Lys Asn Lys Ser Ser Arg Thr Arg Val Ala Ile Leu Gly Ser Gly
1 5 10 15
Ser Ile Gly Leu Asp Leu Met Phe Lys Val Lys Ala Ser Glu His Phe
20 25 30
Asp Leu Lys Phe Val Val Gly Arg His Ala Asn Ser Asp Gly Leu Lys
35 40 45
Leu Ala Arg Ser Cys Asn Val Glu Thr Ser Ser Asp Gly Leu Asp Phe
50 55 60
Leu Lys Glu Asn Glu Asp Ala Tyr Asp Leu Val Phe Asp Ala Thr Ser
65 70 75 80
Ala Ala Ala His Lys Val Asn Asn Gly Phe Phe Ser Gly Ala Gly Lys
85 90 95
Phe Val Ile Asp Leu Thr Pro Ala Lys Leu Gly Arg Leu Cys Val Pro
100 105 110
Cys Ile Asn Leu Asp Asp Ile Gly Ala Glu Gln Asn Val Asn Leu Ile
115 120 125
Thr Cys Gly Gly Gln Ala Ser Leu Pro Leu Ala Tyr Ala Leu Lys Gln
130 135 140
Ala Val Asp Glu Ile Glu Tyr Leu Glu Val Val Ser Ala Ile Ala Ser
145 150 155 160
Arg Ser Ala Gly Ile Ala Thr Arg Glu Asn Ile Asp Glu Tyr Met Thr
165 170 175
Thr Thr Glu Tyr Ala Leu Ala Gln Phe Ser Gly Ala Lys Lys Thr Lys
180 185 190
Ala Ile Leu Asn Ile Asn Pro Ala Glu Pro Gly Val Arg Met Gln Thr
195 200 205
Thr Leu Tyr Ala His Ala Arg Tyr Arg Asp Phe Asp Arg Val Arg Ala
210 215 220
Cys Val Ala Glu Met Val Glu Lys Val Arg Glu Tyr Val Pro Gly Tyr
225 230 235 240
Arg Leu Val Val Glu Pro Ile Glu Ser Gln Gly Arg Ile Thr Ile Ser
245 250 255
Leu Thr Val Arg Gly Arg Gly Asp Tyr Leu Pro Glu Tyr Ala Gly Asn
260 265 270
Leu Asp Ile Ile Asn Cys Ala Ala Leu Ala Val Ala Ser His Arg His
275 280 285
Ala Thr Ala Arg Leu Gly Ala Thr Gln
290 295
<210> 142
<211> 316
<212> PRT
<213> Pseudomonas putida Acylating aldehyde dehydrogenase (Q9EVJ2)
<400> 142
Met Thr Arg Lys Val Lys Ala Ala Ile Ile Gly Ser Gly Asn Ile Gly
1 5 10 15
Thr Asp Leu Met Ile Lys Ile Leu Arg His Gly Gln His Ile Glu Met
20 25 30
Gly Ala Met Val Gly Ile Asp Pro Ala Ser Asp Gly Leu Ala Arg Ala
35 40 45
Gln Arg Met Gly Val Ala Ile Thr His Glu Gly Val Glu Gly Leu Thr
50 55 60
Arg Leu Pro Val Phe Asn Glu Ile Asp Val Val Phe Asp Ala Thr Ser
65 70 75 80
Ala Gly Ala His Val Lys Asn Glu Ala Leu Leu Arg Glu Arg Lys Pro
85 90 95
Gly Leu Arg Met Ile Asp Leu Thr Pro Ala Ala Ile Gly Pro Tyr Cys
100 105 110
Ile Pro Val Val Asn Gly Asp Asp His Leu Asp Ala Thr Asn Val Asn
115 120 125
Met Val Thr Cys Gly Gly Gln Ala Thr Ile Pro Met Val Ala Ala Val
130 135 140
Ser Arg Val Ala Lys Val His Tyr Ala Glu Ile Ile Ala Ser Ile Ser
145 150 155 160
Ser Lys Ser Ala Gly Pro Gly Thr Arg Ala Asn Ile Asp Glu Phe Thr
165 170 175
Glu Thr Thr Ser Lys Ala Ile Glu Val Val Gly Gly Ala Ala Lys Gly
180 185 190
Lys Ala Ile Ile Val Leu Asn Pro Ala Glu Pro Pro Leu Met Met Arg
195 200 205
Asp Thr Val Tyr Thr Leu Ser Asp Phe Ala Asp Ile Asp Gln Ile Glu
210 215 220
Glu Ser Val Gln Arg Met Ala Asp Ala Val Gln Ala Tyr Val Pro Gly
225 230 235 240
Tyr Arg Leu Lys Gln Arg Val Gln Phe Asp Arg Ile Glu Ala Asp Cys
245 250 255
Pro Ile Arg Ile Pro Gly Val Gly Asp Arg Met Asn Gly Leu Lys Thr
260 265 270
Ser Ile Phe Leu Glu Val Glu Gly Ala Ala His Tyr Leu Pro Ala Tyr
275 280 285
Ala Gly Asn Leu Asp Ile Met Thr Ser Ala Ala Leu Arg Thr Ala Glu
290 295 300
Lys Leu Ala Glu Arg Leu Leu Ala Ser Leu Val Ala
305 310 315
<210> 143
<211> 539
<212> PRT
<213> Burkholderia mallei Acetaldehyde dehydrogenase (Acetylating)(A5XRR2)
<400> 143
Met His Arg Gly Ala Ala Cys Trp His Gly Phe Gly Val Thr Asn Gly
1 5 10 15
Val Ala His Val Ala Arg Arg Ile Thr Asp Thr Asp Met Arg Gly Ala
20 25 30
Thr Met Asn His Ala Asp Met Gln His Leu Asn Ile Glu Phe Pro Tyr
35 40 45
Arg Lys Gln Tyr Gly Asn Phe Ile Gly Gly Glu Trp Val Ala Pro Val
50 55 60
Gly Gly Glu Tyr Phe Asp Asn Val Ser Pro Val Thr Gly Arg Pro Phe
65 70 75 80
Thr Ala Ile Pro Arg Ser Arg Glu Ala Asp Ile Glu Leu Ala Leu Asp
85 90 95
Ala Ala His Ala Ala Lys Ala Gly Trp Ala Ala Lys Gly Ala Ala Glu
100 105 110
Arg Ala Asn Val Leu Leu Arg Ile Ala Asp Arg Met Glu Ala Asn Leu
115 120 125
Thr Arg Leu Ala Val Ala Glu Thr Ile Asp Asn Gly Lys Pro Leu Arg
130 135 140
Glu Thr Thr Ala Ala Asp Val Pro Leu Ala Ile Asp His Phe Arg Tyr
145 150 155 160
Phe Ala Gly Cys Ile Arg Ala Gln Glu Gly Ser Ile Ala Asp Ile Gly
165 170 175
Gly Asp Met Val Ala Tyr His Phe His Glu Pro Leu Gly Val Val Gly
180 185 190
Gln Ile Ile Pro Trp Asn Phe Pro Leu Leu Met Ala Ala Trp Lys Leu
195 200 205
Ala Pro Ala Leu Ala Ala Gly Asn Cys Val Val Leu Lys Pro Ala Glu
210 215 220
Gln Thr Pro Ala Ser Ile Leu Val Phe Ala Glu Leu Ile Gln Asp Leu
225 230 235 240
Leu Pro Pro Gly Val Leu Asn Ile Val Asn Gly Phe Gly Leu Glu Ala
245 250 255
Gly Lys Pro Leu Ala Ser Ser Lys Arg Ile Ala Lys Ile Ala Phe Thr
260 265 270
Gly Glu Thr Ser Thr Gly Arg Leu Ile Met Gln Tyr Ala Ser Glu Asn
275 280 285
Leu Ile Pro Val Thr Leu Glu Leu Gly Gly Lys Ser Pro Asn Ile Phe
290 295 300
Phe Ala Asp Val Met Asp Arg Asp Asp Ser Tyr Phe Asp Lys Ala Leu
305 310 315 320
Glu Gly Phe Ala Met Phe Ala Leu Asn Gln Gly Glu Val Cys Thr Cys
325 330 335
Pro Ser Arg Ala Leu Val Glu Glu Ser Ile Tyr Asp Arg Phe Ile Glu
340 345 350
Arg Ala Leu Lys Arg Val Glu Ala Ile Lys Gln Gly His Pro Leu Asp
355 360 365
Ser Gln Thr Met Ile Gly Ala Gln Ala Ser Ala Glu Gln Leu Glu Lys
370 375 380
Ile Leu Ser Tyr Ile Asp Ile Gly Arg Gly Glu Gly Ala Gln Cys Leu
385 390 395 400
Thr Gly Gly Glu Arg Asn Val Leu Gly Gly Glu Leu Ala Glu Gly Tyr
405 410 415
Tyr Val Lys Pro Thr Val Phe Arg Gly His Asn Lys Met Arg Ile Phe
420 425 430
Gln Glu Glu Ile Phe Gly Pro Val Leu Ala Val Thr Thr Phe Lys Thr
435 440 445
Glu Glu Glu Ala Leu Glu Ile Ala Asn Asp Thr Leu Tyr Gly Leu Gly
450 455 460
Ala Gly Val Trp Thr Arg Asp Gly Asn Arg Ala Tyr Arg Phe Gly Arg
465 470 475 480
Gly Ile Gln Ala Gly Arg Val Trp Thr Asn Cys Tyr His Ala Tyr Pro
485 490 495
Ala His Ala Ala Phe Gly Gly Tyr Lys Gln Ser Gly Ile Gly Arg Glu
500 505 510
Thr His Lys Met Met Leu Asp His Tyr Gln Gln Thr Lys Asn Leu Leu
515 520 525
Val Ser Tyr Ser Glu Lys Pro Leu Gly Phe Phe
530 535
<210> 144
<211> 464
<212> PRT
<213> Salmonella typhimurium Propanediol utilization CoA-dependentpropionaldehyde dehydrogenase (Q9XDN1)
<400> 144
Met Asn Thr Ser Glu Leu Glu Thr Leu Ile Arg Thr Ile Leu Ser Glu
1 5 10 15
Gln Leu Thr Thr Pro Ala Gln Thr Pro Val Gln Pro Gln Gly Lys Gly
20 25 30
Ile Phe Gln Ser Val Ser Glu Ala Ile Asp Ala Ala His Gln Ala Phe
35 40 45
Leu Arg Tyr Gln Gln Cys Pro Leu Lys Thr Arg Ser Ala Ile Ile Ser
50 55 60
Ala Met Arg Gln Glu Leu Thr Pro Leu Leu Ala Pro Leu Ala Glu Glu
65 70 75 80
Ser Ala Asn Glu Thr Gly Met Gly Asn Lys Glu Asp Lys Phe Leu Lys
85 90 95
Asn Lys Ala Ala Leu Asp Asn Thr Pro Gly Val Glu Asp Leu Thr Thr
100 105 110
Thr Ala Leu Thr Gly Asp Gly Gly Met Val Leu Phe Glu Tyr Ser Pro
115 120 125
Phe Gly Val Ile Gly Ser Val Ala Pro Ser Thr Asn Pro Thr Glu Thr
130 135 140
Ile Ile Asn Asn Ser Ile Ser Met Leu Ala Ala Gly Asn Ser Ile Tyr
145 150 155 160
Phe Ser Pro His Pro Gly Ala Lys Lys Val Ser Leu Lys Leu Ile Ser
165 170 175
Leu Ile Glu Glu Ile Ala Phe Arg Cys Cys Gly Ile Arg Asn Leu Val
180 185 190
Val Thr Val Ala Glu Pro Thr Phe Glu Ala Thr Gln Gln Met Met Ala
195 200 205
His Pro Arg Ile Ala Val Leu Ala Ile Thr Gly Gly Pro Gly Ile Val
210 215 220
Ala Met Gly Met Lys Ser Gly Lys Lys Val Ile Gly Ala Gly Ala Gly
225 230 235 240
Asn Pro Pro Cys Ile Val Asp Glu Thr Ala Asp Leu Val Lys Ala Ala
245 250 255
Glu Asp Ile Ile Asn Gly Ala Ser Phe Asp Tyr Asn Leu Pro Cys Ile
260 265 270
Ala Glu Lys Ser Leu Ile Val Val Glu Ser Val Ala Glu Arg Leu Val
275 280 285
Gln Gln Met Gln Thr Phe Gly Ala Leu Leu Leu Ser Pro Ala Asp Thr
290 295 300
Asp Lys Leu Arg Ala Val Cys Leu Pro Glu Gly Gln Ala Asn Lys Lys
305 310 315 320
Leu Val Gly Lys Ser Pro Ser Ala Met Leu Glu Ala Ala Gly Ile Ala
325 330 335
Val Pro Ala Lys Ala Pro Arg Leu Leu Ile Ala Leu Val Asn Ala Asp
340 345 350
Asp Pro Trp Val Thr Ser Glu Gln Leu Met Pro Met Leu Pro Val Val
355 360 365
Lys Val Ser Asp Phe Asp Ser Ala Leu Ala Leu Ala Leu Lys Val Glu
370 375 380
Glu Gly Leu His His Thr Ala Ile Met His Ser Gln Asn Val Ser Arg
385 390 395 400
Leu Asn Leu Ala Ala Arg Thr Leu Gln Thr Ser Ile Phe Val Lys Asn
405 410 415
Gly Pro Ser Tyr Ala Gly Ile Gly Val Gly Gly Glu Gly Phe Thr Thr
420 425 430
Phe Thr Ile Ala Thr Pro Thr Gly Glu Gly Thr Thr Ser Ala Arg Thr
435 440 445
Phe Ala Arg Ser Arg Arg Cys Val Leu Thr Asn Gly Phe Ser Ile Arg
450 455 460
<210> 145
<211> 315
<212> PRT
<213> Rhodococcus sp. (strain RHA1) Acetaldehyde dehydrogenase (Q0SJD4)
<400> 145
Met Ser Thr Thr Lys Val Ala Val Ile Gly Ser Gly Asn Ile Gly Thr
1 5 10 15
Asp Leu Val Ile Lys Leu Glu Gln Val Ala Lys Asn Val Glu Ile Ala
20 25 30
Val Leu Val Gly Ile Asp Pro Ala Ser Asp Gly Leu Ala Arg Ala Arg
35 40 45
Arg Leu Gly Ile Ser Thr Val Asp Thr Gly Val Gln Gly Leu Ile Glu
50 55 60
His Pro Glu Phe Asp Asp Ile Glu Ile Ile Phe Asp Ser Thr Ser Ala
65 70 75 80
Lys Ala His Leu Thr Asn Ala Glu Ala Leu Arg Pro Tyr Gly Lys His
85 90 95
Leu Ile Asp Leu Thr Pro Ala Ala Ile Gly Pro Tyr Val Val Pro Ala
100 105 110
Val Asn Leu Asp Gln His Leu Gly Ala Pro Asp Val Asn Met Val Thr
115 120 125
Cys Gly Gly Gln Ala Thr Ile Pro Ile Val Ala Ala Ile Ser Lys Val
130 135 140
Thr Asp Val His Tyr Ala Glu Ile Val Ala Ser Ile Ala Ser Lys Ser
145 150 155 160
Ala Gly Pro Gly Thr Arg Ala Asn Ile Asp Glu Phe Thr Glu Thr Thr
165 170 175
Ala Lys Ala Ile Glu Glu Val Gly Gly Ala Ala His Gly Lys Ala Ile
180 185 190
Ile Val Leu Asn Pro Ala Asp Pro Pro Leu Ile Met Arg Asp Thr Val
195 200 205
Leu Ala Leu Val Ser Asn Pro Asp Gln Asp Ala Ile Arg Gln Ser Ile
210 215 220
Thr Asp Met Val Ala Thr Val Ala Ala Tyr Val Pro Gly Tyr Arg Leu
225 230 235 240
Lys Gln Asp Val Gln Phe Thr Glu Leu Gly Gly Ala Asp Thr Val Ala
245 250 255
Thr Leu Thr Gly Gly Ala Asp Ile Gly Ser Pro Val Trp Lys Val Ser
260 265 270
Val Phe Leu Glu Val Glu Gly Ala Ala His Tyr Leu Pro Ala Tyr Ala
275 280 285
Gly Asn Leu Asp Ile Met Thr Ser Ala Ala Leu Gln Val Ala Glu Lys
290 295 300
Leu Ala Glu Arg Thr Ala Leu Glu Ile Ala Arg
305 310 315
<210> 146
<211> 468
<212> PRT
<213> Clostridium beijerinckii Coenzyme A acylating aldehydedehydrogenase (Q9X681)
<400> 146
Met Asn Lys Asp Thr Leu Ile Pro Thr Thr Lys Asp Leu Lys Leu Lys
1 5 10 15
Thr Asn Val Glu Asn Ile Asn Leu Lys Asn Tyr Lys Asp Asn Ser Ser
20 25 30
Cys Phe Gly Val Phe Glu Asn Val Glu Asn Ala Ile Asn Ser Ala Val
35 40 45
His Ala Gln Lys Ile Leu Ser Leu His Tyr Thr Lys Glu Gln Arg Glu
50 55 60
Lys Ile Ile Thr Glu Ile Arg Lys Ala Ala Leu Glu Asn Lys Glu Val
65 70 75 80
Leu Ala Thr Met Ile Leu Glu Glu Thr His Met Gly Arg Tyr Glu Asp
85 90 95
Lys Ile Leu Lys His Glu Leu Val Ala Lys Tyr Thr Pro Gly Thr Glu
100 105 110
Asp Leu Thr Thr Thr Ala Trp Ser Gly Asp Asn Gly Leu Thr Val Val
115 120 125
Glu Met Ser Pro Tyr Gly Val Ile Gly Ala Ile Thr Pro Ser Thr Asn
130 135 140
Pro Thr Glu Thr Val Ile Cys Asn Ser Ile Gly Met Ile Ala Ala Gly
145 150 155 160
Asn Ala Val Val Phe Asn Gly His Pro Gly Ala Lys Lys Cys Val Ala
165 170 175
Phe Ala Ile Glu Met Ile Asn Lys Ala Ile Ile Ser Cys Gly Gly Pro
180 185 190
Glu Asn Leu Val Thr Thr Ile Lys Asn Pro Thr Met Glu Ser Leu Asp
195 200 205
Ala Ile Ile Lys His Pro Leu Ile Lys Leu Leu Cys Gly Thr Gly Gly
210 215 220
Pro Gly Met Val Lys Thr Leu Leu Asn Ser Gly Lys Lys Ala Ile Gly
225 230 235 240
Ala Gly Ala Gly Asn Pro Pro Val Ile Val Asp Asp Thr Ala Asp Ile
245 250 255
Glu Lys Ala Gly Lys Ser Ile Ile Glu Gly Cys Ser Phe Asp Asn Asn
260 265 270
Leu Pro Cys Ile Ala Glu Lys Glu Val Phe Val Phe Glu Asn Val Ala
275 280 285
Asp Asp Leu Ile Ser Asn Met Leu Lys Asn Asn Ala Val Ile Ile Asn
290 295 300
Glu Asp Gln Val Ser Lys Leu Ile Asp Leu Val Leu Gln Lys Asn Asn
305 310 315 320
Glu Thr Gln Glu Tyr Phe Ile Asn Lys Lys Trp Val Gly Lys Asp Ala
325 330 335
Lys Leu Phe Ser Asp Glu Ile Asp Val Glu Ser Pro Ser Asn Ile Lys
340 345 350
Cys Ile Val Cys Glu Val Asn Ala Asn His Pro Phe Val Met Thr Glu
355 360 365
Leu Met Met Pro Ile Leu Pro Ile Val Arg Val Lys Asp Ile Asp Glu
370 375 380
Ala Val Lys Tyr Thr Lys Ile Ala Glu Gln Asn Arg Lys His Ser Ala
385 390 395 400
Tyr Ile Tyr Ser Lys Asn Ile Asp Asn Leu Asn Arg Phe Glu Arg Glu
405 410 415
Ile Asp Thr Thr Ile Phe Val Lys Asn Ala Lys Ser Phe Ala Gly Val
420 425 430
Gly Tyr Glu Ala Glu Gly Phe Thr Thr Phe Thr Ile Ala Gly Ser Thr
435 440 445
Gly Glu Gly Ile Thr Ser Ala Arg Asn Phe Thr Arg Gln Arg Arg Cys
450 455 460
Val Leu Ala Gly
465
<210> 147
<211> 475
<212> PRT
<213> Bacillus thuringiensis serovar israelensis Alcohol dehydrogenase /Acetaldehyde dehydrogenase (Acetylating) (Q3EZP1)
<400> 147
Met Thr Asn Lys Thr Asp Glu Lys Glu Val Gln Ala Met Val Asn Val
1 5 10 15
Val Ile Lys Asp Glu Asn Asp Val Gln Glu Val Val Asn Thr Leu Val
20 25 30
Glu Asn Gly Lys Glu Ala Leu Lys Ala Leu Glu Ser Tyr Thr Gln Glu
35 40 45
Gln Val Asp His Ile Val His Glu Met Ala Leu Ser Gly Leu Asp Gln
50 55 60
His Met Pro Leu Ala Lys Met Ala Val Glu Glu Thr Gly Arg Gly Val
65 70 75 80
Tyr Glu Asp Lys Cys Thr Lys Asn Ile Phe Ala Thr Glu Tyr Ile Trp
85 90 95
His Ser Ile Lys Lys Asp Lys Thr Val Gly Ile Ile His Glu Asp Pro
100 105 110
His Glu Glu Val Ile Glu Ile Ala Glu Pro Val Gly Val Val Ala Gly
115 120 125
Val Thr Pro Val Thr Asn Pro Thr Ser Thr Thr Met Phe Lys Ala Ile
130 135 140
Ile Ala Met Lys Thr Arg Asn Pro Ile Ile Phe Ala Phe His Pro Ser
145 150 155 160
Ala Gln Gln Cys Ser Val Ala Ala Ala Lys Ile Leu Arg Asp Ala Ala
165 170 175
Ile Arg Ala Gly Ala Pro Glu Asn Cys Ile Gln Trp Ile Glu Lys Pro
180 185 190
Ser Val Glu Ala Thr Lys Arg Leu Met Asn His Glu Gly Val Ala Leu
195 200 205
Val Leu Ala Thr Gly Gly Ala Gly Met Val Lys Ser Ala Tyr Ser Thr
210 215 220
Gly Lys Pro Ala Leu Gly Val Gly Pro Gly Asn Val Pro Cys Tyr Leu
225 230 235 240
Glu Lys Ser Ala His Val Lys Arg Ala Val Asn Asp Leu Ile Leu Ser
245 250 255
Lys Thr Phe Asp Asn Gly Met Ile Cys Ala Ser Glu Gln Ala Ile Ile
260 265 270
Val Asp Lys Glu Ile Tyr Asn Gly Val Lys Lys Glu Met Gln Asp Asn
275 280 285
Asn Cys Tyr Phe Val Thr Glu Glu Glu Arg Ile Lys Leu Glu Lys Leu
290 295 300
Val Ile Asn Glu Asn Thr Cys Ala Val Asn Ser Asp Ile Val Gly Lys
305 310 315 320
Ser Ala His Tyr Ile Ala Ser Leu Val Ser Ile Lys Val Pro Glu Asp
325 330 335
Thr Lys Ile Leu Val Ala Glu Ile Lys Gly Val Gly Ala Glu Tyr Pro
340 345 350
Leu Ser Arg Glu Lys Leu Ser Pro Val Leu Ala Cys Ile Lys Ala Asn
355 360 365
Ser Gln Glu Glu Gly Phe Lys Tyr Cys Glu Glu Met Leu Asn Leu Gly
370 375 380
Gly Leu Gly His Ser Ala Val Ile His Ser Thr Asn Lys Glu Val Gln
385 390 395 400
Lys Gln Phe Gly Leu Arg Met Lys Ala Cys Arg Leu Ile Val Asn Ser
405 410 415
Pro Ser Ala Gln Gly Gly Ile Gly Asp Ile Tyr Asn Ala Phe Ile Pro
420 425 430
Ser Leu Thr Leu Gly Cys Gly Ser Tyr Gly Lys Asn Ser Val Ser Gln
435 440 445
Asn Val Thr Ala Thr His Ile Ile Asn Val Lys Arg Leu Ala Asn Ser
450 455 460
Pro Asp Asn Met Arg Gly Phe Thr Ile Thr Thr
465 470 475
<210> 148
<211> 467
<212> PRT
<213> marine gamma proteobacterium Aldehyde dehydrogenase (NAD) familyprotein (B7RWJ8)
<400> 148
Met Ser Glu Ala Gln Ser Pro Ala Gln Glu Val Ala Glu Met Met Ser
1 5 10 15
Arg Ala Arg Ala Ala Gln Ala Gln Ile Ala Asp Tyr Thr Gln Glu Gln
20 25 30
Val Asp Glu Leu Ile Thr Ala Met Val Tyr Ala Val Ala Arg Glu Asp
35 40 45
Arg Ser Glu Glu Ile Ala Arg Phe Thr Val Glu Glu Thr Gln Leu Gly
50 55 60
Asn Tyr Glu Gly Lys Tyr Leu Lys Ile His Arg Lys Thr Arg Ala Thr
65 70 75 80
Leu Met Asp Ile Ile Asp Asp Lys Ser Val Gly Val Ile Glu Glu Leu
85 90 95
Pro Glu Arg Asn Ile Val Lys Ile Ala Lys Pro Val Gly Val Ile Gly
100 105 110
Ala Leu Ser Pro Ser Thr Asn Pro Glu Ala Thr Pro Val Ile Lys Ala
115 120 125
Ile Ser Ala Val Lys Gly Arg Asn Ala Ile Ile Val Ala Pro His Pro
130 135 140
Arg Ala Lys Leu Thr Asn Lys Lys Ile Cys Asp Tyr Met Arg Glu Ala
145 150 155 160
Leu Glu Leu Cys Gly Ala Pro Ala Asp Leu Val Gln Ser Ile Asp Val
165 170 175
Pro Ser Leu Asp Lys Thr Asn Glu Leu Met Ala Gln Cys Asp Arg Val
180 185 190
Leu Ala Thr Gly Gly Glu Ala Met Val Thr Ala Ala Tyr Ser Ser Gly
195 200 205
Thr Pro Ala Leu Gly Val Gly Val Gly Asn Ala Val Ile Thr Val Asp
210 215 220
Asp Thr Ala Asp Leu Asp Glu Ala Ala Glu Lys Ile Arg Ile Ser Lys
225 230 235 240
Thr Leu Asp Leu Ala Ala Ser Cys Ser Ser Asp Asn Ser Val Leu Val
245 250 255
Phe Glu Ser Val Tyr Asn Glu Leu Leu Glu Lys Leu Lys His Glu Gly
260 265 270
Gly Phe Val Ile Glu Gly Asp Asp Lys Ala Lys Leu Gln Ser Val Ile
275 280 285
Trp Glu Asp Asp His Leu Asn Ala Lys Ile Val Ala Gln His Ala Gln
290 295 300
Thr Ile Cys Gly Gln Ala Gly Ile Asp Leu Pro Glu Gly Lys Ser Phe
305 310 315 320
Leu Ile Val Pro Glu Thr Gly Ala Gly Ala Asp His Pro Phe Ser Gly
325 330 335
Glu Lys Leu Thr Val Thr Met Ala Leu Tyr Lys Val Lys Asp Ile Asp
340 345 350
Glu Ala Ile Glu Met Thr Asn Lys Ile Gln Ala Tyr Gln Gly Gln Gly
355 360 365
His Ser Cys Gly Ile Tyr Ser Tyr Asn Asp Asp Asn Val Leu Lys Leu
370 375 380
Ala Asp Ala Thr Arg Thr Ser Arg Val Met Val Asn Gln Pro Gln Ala
385 390 395 400
Ala Ser Asn Ser Gly Asn Leu Trp Asn Gly Met Arg Gln Thr Phe Ser
405 410 415
Leu Gly Cys Gly Ser Trp Gly Gly Asn Ser Ile Asn His Asn Ile Thr
420 425 430
Trp Arg Asp Leu Ile Asn Glu Thr Trp Val Ser Lys Pro Leu Ala Thr
435 440 445
Thr Lys Thr Ile Pro Ser Asp Glu Glu Leu Phe Gly Ala Val Met Ala
450 455 460
Lys Phe Ser
465

Claims (31)

1.用于制备己二酸酯或己二酸硫代酯的方法，所述方法包括：在存在生物催化剂时将2,3-脱氢己二酸酯或2,3-脱氢己二酸硫代酯转化成己二酸酯或硫代酯。
2.根据权利要求1的方法，其中所述生物催化剂包含下述酶，所述酶能够催化2,3-烯酸酯部分或2-烯酰基部分的碳-碳双键的还原。
3.根据权利要求2的方法，其中所述生物催化剂包含下述酶，所述酶选自作用于供体的HC-CH基团上的氧化还原酶(EC 1.3.1.或1.3.99)的组，优选地选自氧化还原酶(EC 1.3.1和EC 1.3.99)的组，优选地选自烯酰基-CoA还原酶EC 1.3.1.8、EC 1.3.1.38和EC1.3.1.44的组，选自烯酰基-[酰基-运载体-蛋白质]还原酶EC 1.3.1.9、EC 1.3.1.10和EC1.3.1.39的组，以及选自丁酰-CoA脱氢酶(EC 1.3.99.2)、酰基-CoA脱氢酶(1.3.99.3)和长链-酰基-CoA脱氢酶(EC 1.3.99.13)的组。
4.根据前述权利要求中任一项的方法，其中所述生物催化剂包含酶，所述酶包含选自SEQUENCE ID 42-67、94、96、98、100、102、103、105、106、107、109、111、113、115、116中任何所示的氨基酸序列及其同源物的组的氨基酸序列，尤其是选自SEQUENCE ID 60、63、96、100中任何所示的氨基酸序列及其同源物的组的氨基酸序列。
5.根据前述权利要求中任一项的方法，其中所述生物催化剂包含下述生物的酶，所述生物选自Escherichia(尤其是E.coli)，Vibrio，Bacillus(尤其是B.subtilis)，Clostridia(尤其是C.kluyveri，C.acetobutylicum和C.perfringens)，Streptomyces(尤其是S.coelicolor和S.avermitilis)，Pseudomonas(尤其是P.putida和P.aeruginosa)，Shewanella，Xanthomonas，Xylella，Yersinia，Treponema(尤其是T.denticola)，Eubacterium(尤其是E.pyruvativorans)，Micorscilla(尤其是Micorscilla marina)，Aeromonas(尤其是Aeromonas hydrophila)，Megasphera(尤其是Megasphera elsdenii)，Acinetobacter sp.，Deinococcus(尤其是Deinococcus radiourans)，Yarrowia(尤其是Yarrowia lypolytica)，Euglenozoa(尤其是Euglena gracilis)，Saccharomyces(尤其是S.cerevisiae)，Kluyveromyces(尤其是K.lactis)，Schizosaccharomyces(尤其是S.pombe)，Candida(尤其是C.tropicalis)，Aspergillus(尤其是A.niger和A.nidulans)，Penicillium(尤其是P.chrysogenum)，Arabidopsis(尤其是A.thaliana)，Homo sapiens，Rattus norvegicus，Bos Taurus，Cavia sp.，Caenorhabditis elegans和Drosophilamelanogaster的组。
6.根据前述权利要求中任一项的方法，其中通过转化3-羟基己二酸酯或3-羟基己二酸硫代酯来制备2,3-脱氢己二酸酯或2,3-脱氢己二酸硫代酯。
7.根据权利要求6的方法，其中所述3-羟基己二酸酯或硫代酯在存在下述生物催化剂时被生物催化性转化，所述生物催化剂能够催化3-羟基酰基酯或3-羟基酰基硫代酯成为2-烯酰基酯或硫代酯，优选地是包含下述酶的生物催化剂，所述酶选自水解酶(E.C.4.2.1)的组，优选地选自烯酰-CoA水合酶(EC 4.2.1.17)、3-羟基丁酰基-CoA脱水酶(EC 4.2.1.55)和长链-烯酰-CoA水合酶(EC 4.2.1.74)的组。
8.根据权利要求7的方法，其中所述生物催化剂包含下述生物的酶，所述生物选自Acinetobacter(尤其是Acinetobacter sp.菌株ADP1和A.calcoaceticus)，Alicaligenes(尤其是Alicaligenes D2)，Aspergillus(尤其是A.niger)，Azoarcus(尤其是A.evansii)，Bacillus(尤其是B.halodurans)，Corynebacterium(尤其是C.glutamicum和C.aurantiacum)，E.coli，Flavobacterium，Neurospora(尤其是N.crassa)，Penicillium(尤其是P.chrysogenum)，Pseudomonas(尤其是P.putida和P.fluorescens)，Rhodopseudomonas(尤其是R.palustris)，Rhodococcus(尤其是Rhodococcus sp菌株RHA1)，Aeromonas(尤其是
A.caviae)，Clostridium(尤其是C.acetobutylicumi和C.kluyveri)，Gossypium(尤其是G.hirsutum)，Rhodospirillum(尤其是R.rubrumi)，and Ralstonia(尤其是Ralstoniaeutropha)，Euglenozoa(尤其是Euglena gracilis，Megasphera(尤其是M.elsdenii)和Saccharomyces(尤其是S.cerevisiae)和哺乳动物(尤其是Bos taurus，Homo sapiens，Rattus norvegicus和Sus scrofa)的组。
9.根据权利要求6、7或8中任一项的方法，其中所述3-羟基己二酸酯或硫代酯是通过转化3-氧代己二酸酯或3-氧代己二酸硫代酯来制备的。
10.根据权利要求9的方法，所述方法包括：在存在生物催化剂、尤其是能够催化羰基还原成为醇基或能够催化3-氧代酰基酯或3-氧代酰基硫代酯到相应的3-羟基酰基酯或硫代酯的还原的生物催化剂时，生物催化性转化3-氧代己二酸酯或3-氧代己二酸硫代酯，所述能够催化羰基到醇基的还原或能够催化3-氧代酰基酯或3-氧代酰基硫代酯到相应的3-羟基酰基酯或硫代酯的还原的生物催化剂选自脱氢酶(E.C.1.1.1)的组，优选地选自3-羟基酰基-CoA脱氢酶(EC 1.1.1.35和EC 1.1.1.36)、3-羟基丁酰-CoA脱氢酶(EC 1.1.1.157)、3-羟基庚二酰-CoA脱氢酶(EC 1.1.1.259)和长链-3-羟基酰基-CoA脱氢酶(EC 1.1.1.211)的组。
11.根据权利要求10的方法，其中所述生物催化剂包含下述生物的酶，所述生物选自Acinetobacter(尤其是Acinetobacter sp.菌株ADP1和A.calcoaceticus)，Alicaligenes(尤其是Alicaligenes菌株D2和A.eutrophus)，Arzoarcus(尤其是A.evansii)，Bacillus(尤其是B.halodurans)，Bordetella(尤其是B.pertussis)，Burkholderia(尤其是B.pseudomallei和B.xenovorans)，Corynebacterium(尤其是C.glutamicum，C.aurantiacum和C.efficiens)，Deinococcus(尤其是D.radiodurans)，E.coli，Flavobacterium，Klebsiella(尤其是K.pneumonia)，Pseudomonas(尤其是P.putida和P.fluorescens)，Rhodopseudomonas(尤其是R.palustris)，Rodococcus(尤其是R.erythropolis，R.opacus和Rodococcus sp菌株RHA1)，Aspergillus(尤其是A.niger)，Neurospora(尤其是N.crassa)，Penicillium(尤其是P.chrysogenum)，Saccharomyces，Bostaurus，Rattus norvegicus，Sus scrofa，Homo sapiens.Clostridia(尤其是C.acetobutylicum和C.kluyveri)，Euglenozoa(尤其是)Euglena gracilis，Megasphera(尤其是Megasphera elsdenii)，Ralstonia(尤其是Ralstonia eutropha)和Zoogloea(尤其是Zoogloea ramigera)的组。
12.根据权利要求9、10或11中任一项的方法，其中所述3-氧代己二酸酯或3-氧代己二酸硫代酯是通过使琥珀酸酯或琥珀酸硫代酯与乙酸酯或乙酸硫代酯反应来制备的。
13.根据权利要求12的方法，所述方法包括在存在生物催化剂、优选地存在包含能够转移乙酰基的酶的生物催化剂时，使琥珀酸酯或硫代酯与乙酸酯或硫代酯生物催化地反应，所述酶选自酰基转移酶(E.C.2.3.1)的组，尤其是选自乙酰-CoA:乙酰-CoA C-乙酰转移酶(EC 2.3.1.9)，酰基-CoA:乙酰-CoA C-酰基转移酶(EC 2.3.1.16)和琥珀酰-CoA:乙酰-CoAC琥珀酰转移酶(EC 2.3.1.174)组的酰基转移酶，更尤其是包含如SEQUENCE ID 1-13任一中鉴定的氨基酸序列或其同源物的酶。
14.根据权利要求12或13的方法，其中所述生物催化剂包含下述生物的酶，所述生物选自Acinetobacter(尤其是Acinetobacter sp.菌株ADP1和A.calcoaceticus)，Agrobacterium(尤其是A.tumefaciens)，Alicaligenes(尤其是Alicaligenes strains D2和A.eutrophus)，Arthrobacter，Arzoarcus(尤其是A.evansii)，Azomonas，Azotobacter，Bacillus(尤其是B.halodurans)，Beijerinckia，Bradyrhizobium，Burkholderia，Clostridia(尤其是C.kluyveri)，Comamonas，Corynebacterium(尤其是C.glutamicum和C.aurantiacum)，E.coli，Enterobacter，Flavobacterium，Megasphera(尤其是M.elsdenii)，Norcadia，Pseudomonas(尤其是P.putida，P.aeruginosa和Pseudomonas sp.菌株B13)，Ralstonia(尤其是R.eutropha)，Rhizobium，Rhodopseudomonas(尤其是R.palustris)，Rodococcus(尤其是R.erythropolis，R.opacus，和Rodococcus sp菌株RHA1)，Aspergillus(尤其是A.niger)，Euglenozoa(尤其是Euglena gracilis)，Neurospora(尤其是N.crassa)，Penicillium(尤其是P.chrysogenum)，Rhodotorula，Saccharomyces，Trichosporon(尤其是T.cutaneum)的组。
15.根据前述权利要求中任一项的方法，其中任何所述酯选自生物活化基团的组，尤其是选自辅酶A、可以与酰基或肽基运载体蛋白质结合的磷酸泛酰巯基乙胺、N-乙酰-巯乙胺、甲基-硫代-羟乙酸酯、甲基-巯基-丙酸酯、乙基-巯基-丙酸酯、甲基-巯基-丁酸酯、甲基-巯基-丁酸酯和巯基丙酸酯的组。
16.用于制备己二酸的方法，所述方法包括：在根据前述权利要求中任一项的方法中制备己二酸酯或己二酸硫代酯，并水解根据前述权利要求中任一项的方法中获得的己二酸酯或己二酸硫代酯，其中所述水解优选地由生物催化剂、尤其是包含选自水解酶(EC 3.1.2)组的酶的生物催化剂催化。
17.用于制备己二酸的方法，包括在根据权利要求1-15中任一项的方法中制备己二酸酯或己二酸硫代酯并转移在根据权利要求1-15中任一项的方法中获得的己二酸酯或己二酸硫代酯的活化基团，其中所述活化基团转移由生物催化剂催化，所述生物催化剂优选地包含催化含硫基团转移的酶(EC 2.8)，更优选地来自CoA转移酶(EC 2.8.3)的组。
18.用于制备5-甲酰戊酸酯的方法，包括：在根据权利要求1-15中任一项的方法中制备己二酸酯或己二酸硫代酯，或在根据权利要求16或17的方法中制备己二酸，并将所述己二酸酯、己二酸硫代酯或己二酸转化成5-甲酰戊酸酯。
19.根据权利要求18的方法，其中成为5-甲酰戊酸酯的转化是由生物催化剂、优选地包含下述酶的生物催化剂催化的，所述酶选自氧化还原酶(EC 1.2.1)的组，优选地选自醛脱氢酶(EC 1.2.1.3、EC 1.2.1.4和EC 1.2.1.5)、丙二酸酯-半醛脱氢酶(EC 1.2.1.15)、琥珀酸酯-半醛脱氢酶(EC 1.2.1.16和EC 1.2.1.24)、戊二酸酯半醛脱氢酶(EC 1.2.1.20)、氨基己二酸酯半醛脱氢酶(EC 1.2.1.31)、己二酸酯半醛脱氢酶(EC 1.2.1.63)的组，或选自醛脱氢酶(乙酰化)(EC 1.2.1.10)、脂肪酰基-CoA还原酶(EC 1.2.1.42)、长链-脂肪酰基-CoA还原酶(EC 1.2.1.50)、丁醛脱氢酶(EC 1.2.1.57)和琥珀酸酯半醛脱氢酶(乙酰化)的组。
20.用于制备6-氨基己酸的方法，所述方法包括：在根据权利要求18或19的方法中制备5-甲酰戊酸酯，并将所述5-甲酰戊酸酯转化成6-氨基己酸。
21.根据权利要求20的方法，其中所述转化是由生物催化剂、尤其是能够催化氨基转移和/或还原性氨基化的生物催化剂、优选地包含下述酶的生物催化剂催化的，所述酶选自氨基转移酶(EC 2.6.1)和氨基酸脱氢酶(EC 1.4.1)的组，更优选地选自β-氨基异丁酸:α-酮戊二酸氨基转移酶、β-丙氨酸氨基转移酶、天冬氨酸氨基转移酶、4-氨基-丁酸氨基转移酶(EC 2.6.1.19)、L-赖氨酸6-氨基转移酶(EC 2.6.1.36)、2-氨基己二酸氨基转移酶(EC2.6.1.39)、5-氨基戊酸氨基转移酶(EC 2.6.1.48)、2-氨基己酸氨基转移酶(EC2.6.1.67)、赖氨酸:丙酮酸6-氨基转移酶(EC 2.6.1.71)和赖氨酸-6-脱氢酶(EC1.4.1.18)的组。
22.根据权利要求20或21的方法，其中使用的氨基转移酶包含根据Sequence ID 82、Sequence ID 83、Sequence ID 84、Sequence ID 134、Sequence ID 136、Sequence ID 138的氨基酸序列或任何这些氨基酸序列的同源物。
23.根据权利要求20、21或22的方法，其中所述生物催化剂包含来自下述生物的酶，所述生物选自Vibrio；Pseudomonas；Bacillus；Mercurialis；Asplenium；Ceratonia；哺乳动物；Neurospora；Escherichia；Thermus；Saccharomyces；Brevibacterium；Corynebacterium；Proteus；Agrobacterium；Geobacillus；Acinetobacter；Ralstonia和Salmonella的组。
24.用于制备己内酰胺的方法，所述方法包括在根据权利要求20-23中任一项的方法中制备6-氨基己酸，并环化所述6-氨基己酸，从而形成己内酰胺。
25.宿主细胞，所述宿主细胞包含编码权利要求2、3、4、5、7、8、10、11、13、14、16、17、19、21、22和23任一项中定义的酶的一种或多种核酸序列，优选地包含各自编码权利要求2、3、4、5、7、8、10、11、13、14、16、17、19、21、22和23任一项中定义的不同酶的至少两种核酸序列。
26.根据权利要求25的宿主细胞，所述宿主细胞包含编码Sequence ID 42-67、Sequence ID 74-81、94、96、98、100、102、103、105、107、109、111、113、115、116中的任何序列所示的多肽或其同源物的核酸序列。
27.根据权利要求1-24任一项的方法，所述方法包括将包含生物催化剂的细胞与可发酵的碳源接触，所述生物催化剂可包含根据权利要求25或26的宿主细胞或是不同的生物催化剂，其中所述碳源含有要被转化成要制备的化合物的任何所述化合物，或其中所述细胞从所述碳源制备要被转化成要制备的化合物的化合物。
28.通过多种反应，任选地根据权利要求16或17的方法，从琥珀酸或琥珀酸酯或硫代酯制备己二酸的方法，其中至少一种所述反应由生物催化剂催化。
29.根据权利要求28的方法，所述方法包括：
(1)提供琥珀酸酯或硫代酯，将所述酯或硫代酯与乙酸酯或硫代酯反应，从而形成3-氧代己二酸酯或硫代酯；
(2)氢化所述3-氧代己二酸酯或硫代酯的3-氧代基，从而形成3-羟基己二酸酯或硫代酯；
(3)使所述3-羟基己二酸酯或硫代酯脱水，从而形成2,3-脱氢己二酸酯或硫代酯；
(4)氢化2,3-脱氢己二酸酯或硫代酯的C-C双键，从而形成己二酸酯或硫代酯；和
(5)水解所述酯键或硫代酯键，从而形成己二酸。
30.包含根据SEQUENCE ID 57、68、69、70、71、72、79、85中任一的氨基酸序列及其同源物的多肽。
31.编码根据权利要求30的多肽的多核苷酸。
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