CN109417993A - Middle low temperature rainbow conk strain method of purification - Google Patents
Middle low temperature rainbow conk strain method of purification Download PDFInfo
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- CN109417993A CN109417993A CN201710554519.8A CN201710554519A CN109417993A CN 109417993 A CN109417993 A CN 109417993A CN 201710554519 A CN201710554519 A CN 201710554519A CN 109417993 A CN109417993 A CN 109417993A
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Abstract
The invention discloses a kind of middle low temperature rainbow conk strain methods of purification, acquire, take parent species, purify, educating original seed, turn over cultivar and breeding cultivation 17 DEG C or less carry out, it can screen and purify the middle low temperature rainbow conk strain for being suitble to be cultivated between 12~17 DEG C, the natural cultivation in the greenhouse of winter Sichuan province is adapted to completely, greatlys save the energy；Purification operations are repeatedly carried out, obtained middle low temperature rainbow conk growth is stablized, and the efficiency for sprouting fructification is more than 97%, is suitable for manyzoned polypore bacteria in the cultivation in southern winter.
Description
The present invention relates to a kind of methods of purification for technical field, the method for purification of especially middle low temperature rainbow conk strain.
Background technique rainbow conk is the fructification of On Polyporaceae Corilus versicolor Quel., annual.Keratin to hemicellulose matter,
Stockless is given birth in side, and normal imbricate is storied, and often left and right is connected, and is born in the fructification that stump is broken on rainbow conk face or fallen on wood and often surrounds lotus
Seat shape.Cap semicircle has dense fine hair, differs in length, be in a variety of face such as grey, white, brown, blue, purple, black to shell-like
Color, and constitute the concentricity of moire shape.Rainbow conk has biggish medical value.It also tries out in addition to for hepatitis in liver cancer the country
Prevention and treatment, achieve satisfied effect；Chronic bronchitis, chronic active hepatitis etc. are all had a better effect.
Rainbow conk is mainly wild, is born in a variety of broad leaf tree timbers, falls on wood and branch.Rainbow conk mycelia adaptability is very strong, and 3~31
It DEG C can grow, most suitable is 25~28 DEG C.Manyzoned polypore sporophore optimum growth temperature is 25~30 DEG C.Winter in Sichuan, greenhouse
Temperature in greenhouse is unfavorable for the growth of manyzoned polypore sporophore between 12~17 DEG C.Our unit is located in the Mount Emei foot of a hill or mountain, and dependence is rich
Rich rainbow conk wild resource, achieves successful experience in terms of the artificial cultivation of rainbow conk.
Summary of the invention can be fitted present invention aim to provide a kind of middle low temperature rainbow conk strain method of purification, cultivation
Answer the rainbow conk strain of normal growth under cold environmental conditions.
In order to achieve the above object, middle low temperature rainbow conk strain method of purification provided by the invention, specifically comprises the following steps:
1., acquisition: by the end of October to the wild manyzoned polypore sporophore for arriving field acquisition stalwartness at the beginning of 12 months；By the end of October at the beginning of 12 months
Mount Emei's the foot of a mountain field temperature still has wild manyzoned polypore sporophore to survive at this time between 7~15 DEG C.
2., take parent species: collected wild manyzoned polypore sporophore is taken back into laboratory, in sterile and 15 DEG C of ± 2 DEG C of degree conditions
Under, parent species are obtained by spore or tissue separation；
3., purification: under the conditions of sterile and 15 DEG C of ± 2 DEG C of degree, by parent species on the indoor test tube slant of experiment after growth,
It chooses healthy and strong mycelium and obtains test tube species；
4., educate original seed: test tube species are transferred on culture medium, in 15 DEG C of ± 2 DEG C of degree constant temperature and under the conditions of be protected from light after culture,
It chooses healthy and strong mycelium and obtains original seed；
5., turn over cultivar: by original seed switching in matrix expanding propagation, 15 DEG C of ± 2 DEG C of degree constant temperature be protected from light under the conditions of
After culture, chooses healthy and strong mycelium and obtain cultivar；
6., breeding cultivation: cultivar is inoculated with to and is transplanted greenhouse, grows fructification naturally between 12~17 DEG C,
It is acquired after the disappearance of fructification white edge；
7., repeat to purify: choose the healthy and strong fructification that acquires in previous step, repeat step 2.~step 6., weight
One is answered to secondary, completion purification operations.
Middle low temperature rainbow conk strain method of purification provided by the present invention have significant effect: acquire, take parent species, purification,
Educate original seed, turn over cultivar and breeding cultivation 17 DEG C or less carry out, can screen and purify be suitble between 12~17 DEG C into
The middle low temperature rainbow conk strain of row cultivation adapts to the natural cultivation in the greenhouse of winter Sichuan province completely, greatlys save the energy；
Purification operations are repeatedly carried out, obtained middle low temperature rainbow conk growth is stablized, and the efficiency for sprouting fructification is more than 97%, is fitted
Together in manyzoned polypore bacteria southern winter cultivation.
The present invention is further illustrated below according to specific embodiment for specific embodiment, but is not intended to limit
Protection scope of the present invention.
Embodiment 1: middle low temperature rainbow conk strain method of purification specifically comprises the following steps:
1., acquisition: by the end of October to the wild manyzoned polypore sporophore for arriving field acquisition stalwartness at the beginning of 12 months；By the end of October at the beginning of 12 months
Mount Emei's the foot of a mountain field temperature still has wild manyzoned polypore sporophore to survive at this time between 7~15 DEG C.
2., take parent species: collected wild manyzoned polypore sporophore is taken back into laboratory, sterile and under the conditions of 13 DEG C, is passed through
Spore or tissue separation obtain parent species；
3., purification: under the conditions of sterile and 13 DEG C of degree, by parent species on testing indoor test tube slant after growth, selection
Healthy and strong mycelium obtains test tube species；
4., educate original seed: test tube species are transferred on culture medium, in 13 DEG C of degree constant temperature and under the conditions of be protected from light after culture, are chosen
Healthy and strong mycelium obtains original seed；
5., turn over cultivar: by original seed switching in matrix expanding propagation, 13 DEG C of degree constant temperature be protected from light under the conditions of cultivate
Afterwards, it chooses healthy and strong mycelium and obtains cultivar；
6., breeding cultivation: cultivar is inoculated with to and is transplanted greenhouse, grows fructification naturally between 12~17 DEG C,
It is acquired after the disappearance of fructification white edge；
7., repeat to purify: choose the healthy and strong fructification that acquires in previous step, repeat step 2.~step 6., weight
One is answered to secondary, completion purification operations.
Embodiment 2: middle low temperature rainbow conk strain method of purification specifically comprises the following steps:
1., acquisition: by the end of October to the wild manyzoned polypore sporophore for arriving field acquisition stalwartness at the beginning of 12 months；By the end of October at the beginning of 12 months
Mount Emei's the foot of a mountain field temperature still has wild manyzoned polypore sporophore to survive at this time between 7~15 DEG C.
2., take parent species: collected wild manyzoned polypore sporophore is taken back into laboratory, sterile and under the conditions of 14 DEG C, is passed through
Spore or tissue separation obtain parent species；
3., purification: under the conditions of sterile and 14 DEG C of degree, by parent species on testing indoor test tube slant after growth, selection
Healthy and strong mycelium obtains test tube species；
4., educate original seed: test tube species are transferred on culture medium, in 14 DEG C of degree constant temperature and under the conditions of be protected from light after culture, are chosen
Healthy and strong mycelium obtains original seed；
5., turn over cultivar: by original seed switching in matrix expanding propagation, 14 DEG C of degree constant temperature be protected from light under the conditions of cultivate
Afterwards, it chooses healthy and strong mycelium and obtains cultivar；
6., breeding cultivation: cultivar is inoculated with to and is transplanted greenhouse, grows fructification naturally between 12~17 DEG C,
It is acquired after the disappearance of fructification white edge；
7., repeat to purify: choose the healthy and strong fructification that acquires in previous step, repeat step 2.~step 6., weight
One is answered to secondary, completion purification operations.
Embodiment 3: middle low temperature rainbow conk strain method of purification specifically comprises the following steps:
1., acquisition: by the end of October to the wild manyzoned polypore sporophore for arriving field acquisition stalwartness at the beginning of 12 months；By the end of October at the beginning of 12 months
Mount Emei's the foot of a mountain field temperature still has wild manyzoned polypore sporophore to survive at this time between 7~15 DEG C.
2., take parent species: collected wild manyzoned polypore sporophore is taken back into laboratory, sterile and under the conditions of 15 DEG C, is passed through
Spore or tissue separation obtain parent species；
3., purification: under the conditions of sterile and 15 DEG C of degree, by parent species on testing indoor test tube slant after growth, selection
Healthy and strong mycelium obtains test tube species；
4., educate original seed: test tube species are transferred on culture medium, in 15 DEG C of degree constant temperature and under the conditions of be protected from light after culture, are chosen
Healthy and strong mycelium obtains original seed；
5., turn over cultivar: by original seed switching in matrix expanding propagation, 15 DEG C of degree constant temperature be protected from light under the conditions of cultivate
Afterwards, it chooses healthy and strong mycelium and obtains cultivar；
6., breeding cultivation: cultivar is inoculated with to and is transplanted greenhouse, grows fructification naturally between 12~17 DEG C,
It is acquired after the disappearance of fructification white edge；
7., repeat to purify: choose the healthy and strong fructification that acquires in previous step, repeat step 2.~step 6., weight
One is answered to secondary, completion purification operations.
Embodiment 4: middle low temperature rainbow conk strain method of purification specifically comprises the following steps:
1., acquisition: by the end of October to the wild manyzoned polypore sporophore for arriving field acquisition stalwartness at the beginning of 12 months；By the end of October at the beginning of 12 months
Mount Emei's the foot of a mountain field temperature still has wild manyzoned polypore sporophore to survive at this time between 7~15 DEG C.
2., take parent species: collected wild manyzoned polypore sporophore is taken back into laboratory, sterile and under the conditions of 16 DEG C, is passed through
Spore or tissue separation obtain parent species；
3., purification: under the conditions of sterile and 16 DEG C of degree, by parent species on testing indoor test tube slant after growth, selection
Healthy and strong mycelium obtains test tube species；
4., educate original seed: test tube species are transferred on culture medium, in 16 DEG C of degree constant temperature and under the conditions of be protected from light after culture, are chosen
Healthy and strong mycelium obtains original seed；
5., turn over cultivar: by original seed switching in matrix expanding propagation, 16 DEG C of degree constant temperature be protected from light under the conditions of cultivate
Afterwards, it chooses healthy and strong mycelium and obtains cultivar；
6., breeding cultivation: cultivar is inoculated with to and is transplanted greenhouse, grows fructification naturally between 12~17 DEG C,
It is acquired after the disappearance of fructification white edge；
7., repeat to purify: choose the healthy and strong fructification that acquires in previous step, repeat step 2.~step 6., weight
One is answered to secondary, completion purification operations.
Embodiment 5: middle low temperature rainbow conk strain method of purification specifically comprises the following steps:
1., acquisition: by the end of October to the wild manyzoned polypore sporophore for arriving field acquisition stalwartness at the beginning of 12 months；By the end of October at the beginning of 12 months
Mount Emei's the foot of a mountain field temperature still has wild manyzoned polypore sporophore to survive at this time between 7~15 DEG C.
2., take parent species: collected wild manyzoned polypore sporophore is taken back into laboratory, sterile and under the conditions of 17 DEG C, is passed through
Spore or tissue separation obtain parent species；
3., purification: under the conditions of sterile and 17 DEG C of degree, by parent species on testing indoor test tube slant after growth, selection
Healthy and strong mycelium obtains test tube species；
4., educate original seed: test tube species are transferred on culture medium, in 17 DEG C of degree constant temperature and under the conditions of be protected from light after culture, are chosen
Healthy and strong mycelium obtains original seed；
5., turn over cultivar: by original seed switching in matrix expanding propagation, 17 DEG C of degree constant temperature be protected from light under the conditions of cultivate
Afterwards, it chooses healthy and strong mycelium and obtains cultivar；
6., breeding cultivation: cultivar is inoculated with to and is transplanted greenhouse, grows fructification naturally between 12~17 DEG C,
It is acquired after the disappearance of fructification white edge；
7., repeat to purify: choose the healthy and strong fructification that acquires in previous step, repeat step 2.~step 6., weight
One is answered to secondary, completion purification operations.
According to the middle low temperature rainbow conk strain method of purification of above-described embodiment 1-5, will only take parent species, purify, educate original seed and
The temperature for turning over cultivar changes, remaining content is identical.Each embodiment is all made of 100 bags of progress breeding cultivations, records each reality
The fructification for applying example sprouts result.Breeding cultivation result proves: fructification is sprouted sufficiently, and average germination rate is 98.1%；Growth
Stalwartness, yield when yield is close to summer culture.
The scope of the present invention is related to all the above version.
Claims (1)
1. a kind of middle low temperature rainbow conk strain method of purification, it is characterised in that include the following steps:
1., acquisition: by the end of October to the wild manyzoned polypore sporophore for arriving field acquisition stalwartness at the beginning of 12 months；
2., take parent species: collected wild manyzoned polypore sporophore is taken back into laboratory, under the conditions of sterile and 15 DEG C of ± 2 DEG C of degree, lead to
It crosses spore or tissue separation obtains parent species；
3., purification: under the conditions of sterile and 15 DEG C of ± 2 DEG C of degree, by parent species on testing indoor test tube slant after growth, selection
Healthy and strong mycelium obtains test tube species；
4., educate original seed: test tube species are transferred on culture medium, in 15 DEG C of ± 2 DEG C of degree constant temperature and under the conditions of be protected from light after culture, are chosen
Healthy and strong mycelium obtains original seed；
5., turn over cultivar: by original seed switching in matrix expanding propagation, 15 DEG C of ± 2 DEG C of degree constant temperature be protected from light under the conditions of cultivate
Afterwards, it chooses healthy and strong mycelium and obtains cultivar；
6., breeding cultivation: cultivar is inoculated with to and is transplanted greenhouse, fructification is grown naturally between 12~17 DEG C, to son
Entity white edge acquires after disappearing；
7., repeat to purify: choose the healthy and strong fructification that acquires in previous step, repeat step 2.~step 6., repeat one
To secondary, completion purification operations.
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| CN201710554519.8A CN109417993A (en) | 2017-06-19 | 2017-06-19 | Middle low temperature rainbow conk strain method of purification |
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| CN201710554519.8A CN109417993A (en) | 2017-06-19 | 2017-06-19 | Middle low temperature rainbow conk strain method of purification |
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| CN109417993A true CN109417993A (en) | 2019-03-05 |
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| CN201710554519.8A Pending CN109417993A (en) | 2017-06-19 | 2017-06-19 | Middle low temperature rainbow conk strain method of purification |
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- 2017-06-19 CN CN201710554519.8A patent/CN109417993A/en active Pending
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