CN111316900A - Method for improving transplanting survival rate of virus-free seedlings of purple-meat sweet potatoes - Google Patents
Method for improving transplanting survival rate of virus-free seedlings of purple-meat sweet potatoes Download PDFInfo
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- CN111316900A CN111316900A CN202010224061.1A CN202010224061A CN111316900A CN 111316900 A CN111316900 A CN 111316900A CN 202010224061 A CN202010224061 A CN 202010224061A CN 111316900 A CN111316900 A CN 111316900A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/25—Root crops, e.g. potatoes, yams, beet or wasabi
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/40—Growth substrates; Culture media; Apparatus or methods therefor characterised by their structure
- A01G24/42—Growth substrates; Culture media; Apparatus or methods therefor characterised by their structure of granular or aggregated structure
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
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- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Cultivation Of Plants (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention provides a method for improving the transplanting survival rate of virus-free seedlings of purple sweet potatoes, which belongs to the technical field of crop germplasm innovation and new variety breeding and comprises the following steps: planting the detoxicated purple sweet potato seedlings in vermiculite and hardening. The survival rate of the detoxified seedling of the purple sweet potato transplanted in the vermiculite culture medium is improved and reaches up to 95 percent.
Description
Technical Field
The invention belongs to the technical field of crop germplasm innovation and new variety breeding, and particularly relates to a method for improving the transplanting survival rate of virus-free seedlings of purple sweet potatoes.
Background
The sweet potato is a main food crop and an energy crop in China, contains a large amount of carbohydrates, and is also rich in various nutrient substances such as protein, vitamins, mineral substances, dietary fibers, polysaccharide and the like, and the purple sweet potato is a specific sweet potato type and is named because the root tuber of the purple sweet potato is rich in anthocyanin. With the enhancement of health care consciousness and the improvement of the living standard of people, the purple sweet potato is more and more popular with domestic consumers, which also promotes the continuous expansion of the planting scale of the purple sweet potato. Xu purple sweet potato No. 8 is a new purple sweet potato variety newly bred by Xuzhou agricultural science research institute in Xuhuai region of Jiangsu, and the variety has the advantages of strong adaptability, good earliness, high yield, high anthocyanin content, excellent processing quality and the like, and the planting area of the variety is increasingly large in recent years. However, sweet potato production is seriously compromised due to the fact that sweet potato virus diseases are continuously generated in recent years, so that sweet potato yield is reduced, the properties of sweet potatoes are degraded, and sweet potato production is seriously compromised. The sweet potato virus-free seedling is an effective method for preventing and treating sweet potato virus diseases and improving the yield and quality of the sweet potatoes. However, the growth conditions of the detoxified seedlings in tissue culture are different from the natural environment of the field, the resistance of the tissue culture seedlings is poor, the problems of susceptibility to diseases, poor growth, difficult rooting, low survival rate and the like occur in the transplanting process, the seedling culture cost is high, and a large number of high-quality detoxified seedlings are difficult to obtain for production and use.
Disclosure of Invention
In view of the above, the invention aims to provide a method for improving the transplanting survival rate of the virus-free seedlings of purple sweet potatoes.
In order to achieve the above purpose, the invention provides the following technical scheme:
the invention provides a method for improving the transplanting survival rate of virus-free seedlings of purple sweet potatoes, which comprises the following steps: planting the detoxicated purple sweet potato seedlings in vermiculite and hardening.
Preferably, the root length of the detoxified seedling of purple sweet potato is 3-5 cm.
Preferably, only 2-3 leaves at the top of the detoxified seedling of purple sweet potato are left.
Preferably, the particle size of the vermiculite is 0.5-2 mm.
Preferably, the purple sweet potato virus-free seedlings are planted after the vermiculite is sterilized.
Preferably, the sterilization temperature is 115-120 ℃, and the sterilization time is 18-22 min.
Preferably, the variety of the purple sweet potato virus-free seedling comprises Xupurple sweet potato No. 8.
The invention provides a method for improving the transplanting survival rate of virus-free seedlings of purple sweet potatoes, which comprises the following steps: planting the detoxicated purple sweet potato seedlings in vermiculite and hardening. The survival rate of the detoxified seedling of the purple sweet potato transplanted in the vermiculite culture medium is improved and reaches up to 95 percent.
Drawings
FIG. 1 shows the root growth of Xupurple sweet potato No. 8 virus-free seedlings in different culture mediums, and virus-free seedlings cultured in nutrient mediums, perlite and vermiculite are arranged from left to right in sequence;
FIG. 2 shows the growth of Xupurple sweet potato No. 8 virus-free seedlings in different culture mediums.
Detailed Description
The invention provides a method for improving the transplanting survival rate of virus-free seedlings of purple sweet potatoes, which comprises the following steps: planting the detoxicated purple sweet potato seedlings in vermiculite and hardening.
In the invention, the variety of the purple sweet potato virus-free seedling preferably comprises Xu purple sweet potato No. 8.
In the invention, the root length of the detoxified seedling of the purple sweet potato is preferably 3-5 cm, and the detoxified seedling of the purple sweet potato only leaves 2-3 leaves at the top.
In the invention, the particle size of the vermiculite is preferably 0.5-2 mm. In the invention, the vermiculite is preferably sterilized, and then the purple sweet potato virus-free seedlings are planted, the sterilization temperature is preferably 115-120 ℃, and the sterilization time is preferably 18-22 min.
The invention has no special limitation on the seedling hardening method, and the seedling hardening method of the conventional purple sweet potato virus-free seedlings in the field is adopted.
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
Example 1
1. Selecting virus-free seedlings: selecting strong slow purple sweet potato No. 8 detoxified seedlings with developed root systems, uncovering a sealing film or a bottle cap by half, and placing the seedlings in a culture room for 2 days.
2. Selecting a culture medium: vermiculite (vermiculite, V: vermiculite specially used for common flowers, 0.5-2 mm) is selected and is sterilized in an autoclave at a high temperature of 120 ℃ for 20min before use.
3. Seedling transplanting treatment: carefully clamping the detoxified seedling out of the culture bottle by using a pair of tweezers, cleaning with water to remove the root culture medium, and taking care to prevent the root system from being damaged; cutting off redundant larger leaves and longer root systems, leaving 3cm long roots and only 2 leaves at the tops of the leaves, and then transplanting the potato seedlings into a small flowerpot filled with a substrate after high-temperature disinfection.
4. Hardening seedlings: vertically inserting two thin sticks into the flowerpot, completely wrapping the potato seedlings by using a transparent plastic film, and placing the wrapped potato seedlings indoors for cultivation for 4 days, wherein the wrapped potato seedlings are kept wet by vermiculite all the time, so that survival and root growth of the detoxified seedlings are facilitated; and (3) removing 1/6 or so from the plastic film, expanding the ventilation opening to 1/2 or so after 2 days, completely removing the plastic film after 2 days, normally culturing the virus-free seedlings indoors for 1-2 weeks, and then investigating the transplanting survival rate. The results are shown in FIGS. 1 and 2.
From fig. 1 and 2, it can be seen that the new root system of the sweet potato seedlings planted by vermiculite (V) grows more and longer, the overall growth vigor is good, and the transplanting survival rate is 95%. Therefore, vermiculite with lower cost and higher transplanting survival rate is selected for transplanting and expanding propagation of the sweet potato virus-free seedlings. Then the potato seedlings are moved to the outdoor for 1 week of sunshade culture, and finally can be planted in a field environment.
Comparative example 1
The culture medium is selected from nutrition medium (NM: "Youjia" seedling culture medium nitrogenTotal amount of phosphorus and potassium N + P2O5+K21.0 to 3.5 percent of O and more than or equal to 30 percent of organic matter content), and the rest conditions are the same as those of the example 1, and the results are shown in figures 1 and 2.
As can be seen from the figures 1 and 2, sweet potato seedlings planted by the Nutrient Medium (NM) are died at first, the original root system is rotten, the number of new roots is small, the part of the stems of the sweet potato seedlings, which is in contact with soil, is rotten, and the transplanting survival rate is 35%.
Comparative example 2
Perlite (P: general horticultural perlite, 3-6 mm) was selected as a culture medium, and the conditions were the same as in example 1, and the results are shown in FIGS. 1 and 2.
It can be seen from fig. 1 and 2 that the sweet potato seedlings planted with perlite (P) have withered leaves, the new roots are seriously bent, the new roots are few, and the transplanting survival rate is 65%.
According to the embodiments and the comparative examples, the detoxified seedling of the purple sweet potato is transplanted into the vermiculite to harden the seedling, so that the transplanting survival rate of the detoxified seedling of the purple sweet potato is improved.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (7)
1. A method for improving the transplanting survival rate of virus-free seedlings of purple sweet potatoes is characterized by comprising the following steps: planting the detoxicated purple sweet potato seedlings in vermiculite and hardening.
2. The method of claim 1, wherein the root length of the detoxified seedling of purple sweet potato is 3-5 cm.
3. The method according to claim 1 or 2, wherein the detoxified seedling of purple sweet potato leaves only 2-3 leaves on the top.
4. The method according to claim 1, wherein the particle size of the vermiculite is 0.5-2 mm.
5. The method of claim 1 or 4, wherein the purple sweet potato virus-free seedlings are planted after the vermiculite is sterilized.
6. The method according to claim 5, wherein the temperature of the sterilization is 115-120 ℃, and the time of the sterilization is 18-22 min.
7. The method of claim 1, wherein the variety of sweet potato virus-free seedlings comprises xu purple sweet potato # 8.
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| CN202010224061.1A CN111316900A (en) | 2020-03-26 | 2020-03-26 | Method for improving transplanting survival rate of virus-free seedlings of purple-meat sweet potatoes |
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| CN202010224061.1A CN111316900A (en) | 2020-03-26 | 2020-03-26 | Method for improving transplanting survival rate of virus-free seedlings of purple-meat sweet potatoes |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114982635A (en) * | 2022-06-17 | 2022-09-02 | 紫云自治县紫香源农林科技有限责任公司 | Detoxified miniature sweet potato propagation method |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU1103819A1 (en) * | 1982-12-13 | 1984-07-23 | Белорусский Ордена Трудового Красного Знамени Государственный Университет Им.В.И.Ленина | Method of estimating quality of fruit and vegetables |
| CN105850461A (en) * | 2016-04-25 | 2016-08-17 | 吉林师范大学 | Transplanting method for purple sweet potato seedlings |
| CN106134723A (en) * | 2016-06-30 | 2016-11-23 | 普洱绿海生物开发有限公司 | A kind of detoxification implantation methods of gold zone Radix Ipomoeae |
| CN109392682A (en) * | 2018-12-13 | 2019-03-01 | 江苏徐淮地区徐州农业科学研究所(江苏徐州甘薯研究中心) | A kind of sweet potato tissue culture method for transplanting of high-survival rate |
| CN110771503A (en) * | 2019-11-11 | 2020-02-11 | 山西省农业科学院棉花研究所 | Sugar-free cultivation strong seedling transplanting method for detoxified sweet potato seedlings |
-
2020
- 2020-03-26 CN CN202010224061.1A patent/CN111316900A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU1103819A1 (en) * | 1982-12-13 | 1984-07-23 | Белорусский Ордена Трудового Красного Знамени Государственный Университет Им.В.И.Ленина | Method of estimating quality of fruit and vegetables |
| CN105850461A (en) * | 2016-04-25 | 2016-08-17 | 吉林师范大学 | Transplanting method for purple sweet potato seedlings |
| CN106134723A (en) * | 2016-06-30 | 2016-11-23 | 普洱绿海生物开发有限公司 | A kind of detoxification implantation methods of gold zone Radix Ipomoeae |
| CN109392682A (en) * | 2018-12-13 | 2019-03-01 | 江苏徐淮地区徐州农业科学研究所(江苏徐州甘薯研究中心) | A kind of sweet potato tissue culture method for transplanting of high-survival rate |
| CN110771503A (en) * | 2019-11-11 | 2020-02-11 | 山西省农业科学院棉花研究所 | Sugar-free cultivation strong seedling transplanting method for detoxified sweet potato seedlings |
Non-Patent Citations (1)
| Title |
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| 孙厚俊等: "一种高成活率的甘薯组培苗移栽方法", 《江苏师范大学学报(自然科学版)》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114982635A (en) * | 2022-06-17 | 2022-09-02 | 紫云自治县紫香源农林科技有限责任公司 | Detoxified miniature sweet potato propagation method |
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