CN110771503A - Sugar-free cultivation strong seedling transplanting method for detoxified sweet potato seedlings - Google Patents
Sugar-free cultivation strong seedling transplanting method for detoxified sweet potato seedlings Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
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Abstract
The invention belongs to the field of plant tissue culture, and particularly relates to a sugar-free culture strong seedling transplanting method for sweet potato virus-free seedlings. Carrying out sweet potato virus-free tissue culture in a test tube, proliferating virus-free seedlings, cutting old roots and stem sections of the virus-free seedlings, transplanting the virus-free seedlings into a sugar-free culture box filled with vermiculite, wherein 670g of vermiculite is contained in each box, and pouring 800mL of nutrient solution; the nutrient solution contains 2g/L of calcium ammonium nitrate and 170mg/LKH
2PO
4The balance being deionized water; introducing CO into the culture box
2After culturing for 25 days in the culture box, opening the box cover, performing hardening seedling transition under natural conditions, and moving to a greenhouse after 4 days; taking out the tissue culture seedlings from the culture box and transplanting the tissue culture seedlings into greenhouse soil. The invention satisfies the nutrition required by the growth of the tissue culture seedling, simplifies the procedure and saves the production cost. Maintains the permeability of the matrix, is convenient for the nutrient absorption and the respiration of the root system of the tissue culture seedling, and has the best growth and rooting of the tissue culture seedling.
Description
Technical Field
The invention belongs to the field of plant tissue culture, and particularly relates to a sugar-free culture strong seedling transplanting method for sweet potato virus-free seedlings.
Background
Sweet potatoes are diverse in use and rich in nutrition, are functional food pursued by modern people, the planting area of the sweet potatoes is steadily increased in recent years, but the yield is reduced by 50-80% due to the occurrence of virus diseases in a sweet potato production area, even the sweet potatoes are not harvested, and a large amount of high-quality virus-free healthy seedlings are urgently needed in production for industrial development.
The traditional sweet potato tissue culture rapid propagation is to perform heterotrophic growth in a limited closed small container by taking sugar as a growth carbon source of tissue culture seedlings, and because of high relative humidity in the culture container, CO
2The concentration is low, the photosynthetic capacity of the detoxified seedling is poor, the growth quality and the rooting quality are poor, after the tissue culture seedling is rapidly propagated for many times, yellow and withered leaves appear, the stem tip grows and is listened and died, and then the stem tip grows for two times, so that the growth period is unstable; in addition, the culture with sugar easily causes the pollution of microorganisms. In terms of transplanting, there are two conventional transplanting methods: (1) and (3) opening a bottle cap of the detoxified test-tube plantlet in a laboratory, hardening the detoxified test-tube plantlet for 2-3 days, then transplanting the detoxified test-tube plantlet into a nutrition pot filled with nutrient soil, vermiculite and peat soil, irrigating nutrient solution and adopting a moisture-preserving measure, and transplanting the survived test-tube plantlet into an insect-proof net-shed nursery field about 20 days. (2) The direct soil-entering transplanting method of the sweet potato virus-free test-tube seedling net shed saves the step of transplanting nutrition pots in the conventional method, simplifies the operation process, and strictly controls the requirement on soil humidity to be 13-14%. The two methods have the advantages of multiple management procedures within a period of time after transplantation, need of stabilizing soil and replenishing water in time, labor and time consumption and high cost. The above mentionedMultiple adverse factors seriously restrict the healthy and large-scale development of the sweet potato industry.
The sweet potato sugar-free culture strong seedling transplanting method is creatively established for improving the growth quality and the transplanting survival rate of the sweet potato virus-free tissue culture seedlings and accelerating the rapid propagation process of test-tube seedlings. The sugar-free culture method mainly utilizes CO
2Sugar in the culture medium is replaced to be used as a carbon source for the growth of the tissue culture seedling for autotrophic growth, so that the photosynthetic rate and the growth rate of the tissue culture seedling are promoted. By dynamically adjusting CO
2Concentration, manual control of light, temperature and humidity, optimization of growth environment, improvement of self photosynthetic capacity and external environment adaptation capacity of the detoxified tissue culture seedlings and rooting quality of the tissue culture seedlings, reduction of microbial contamination rate, promotion of robust growth of plants, and improvement of transplanting survival rate, wherein the transplanting survival rate reaches more than 97%.
Disclosure of Invention
The invention provides a sugar-free cultivation strong seedling transplanting method for sweet potato virus-free seedlings, aiming at solving the problems of poor growth quality, serious microbial pollution and low transplanting survival rate of the existing sweet potato virus-free seedlings.
The invention is realized by the following technical scheme: a sugar-free culture strong seedling transplanting method for detoxified seedlings of sweet potatoes comprises the following steps:
(1) carrying out sweet potato virus-free tissue culture in a test tube, proliferating virus-free seedlings, selecting virus-free seedlings growing for 45-55 days, cutting old roots, shearing the virus-free seedlings into 4cm stem sections, transplanting the stem sections into a sugar-free culture box filled with vermiculite, pouring 800mL of nutrient solution into each box filled with 670g of vermiculite, transplanting 70 seedlings into each box, covering a box cover, and sealing a gap between the box cover and the box body by using a sealing film; the nutrient solution contains 2g/L of calcium ammonium nitrate and 170mg/LKH
2PO
4The balance being deionized water;
(2) transplanting, placing on a culture shelf in a closed room, introducing CO into the culture box
2Opening the ventilation hole of the culture box, and introducing CO along with the growth of the culture period
2The amount of (a) gradually becomes larger;
(3) the culture period is not irradiated with light for 2 days, and the irradiation intensity is 80umol/m from day 3
2S, light intensity of 160umol/m was given at the beginning of day 8
2S, light 12 hours per day;
(4) supplementing nutrient solution in time according to the growth condition of the detoxified seedlings;
(5) after culturing for 25 days in the culture box, opening the box cover, performing seedling hardening transition under natural conditions, spraying water on the 3 rd day according to the dryness and humidity of vermiculite, and moving to a greenhouse after 4 days;
(6) taking out the tissue culture seedlings from the culture box and transplanting the tissue culture seedlings into greenhouse soil.
As a further improvement of the technical proposal of the invention, in the step (2), CO in the closed room
2The maximum concentration is 1500PPm and the minimum 1000 PPm.
As a further improvement of the technical scheme of the invention, in the step (2), the CO is not carried out for the first 4 days in the culture period
2Gas supply, start CO on day 5
2The amount of supplied air is 300 to 600ml/min, and CO starts on day 10
2The gas supply amount is 2000ml/min, and CO
2The air supply time of (a) is consistent with the time of giving light.
As a further improvement of the technical scheme of the invention, in the step (6), the soil humidity in the greenhouse is 13-14%, and the environmental humidity is more than 75%.
As a further improvement of the technical scheme of the invention, in the step (6), after the tissue culture seedlings are transplanted to the soil of the greenhouse, a small plastic arch shed is built.
As a further improvement of the technical scheme of the invention, when the temperature in the greenhouse is higher than 32 ℃, a sunshade net with 50 percent of covering density needs to be covered.
As a further improvement of the technical scheme, 5-7 days after the transplanting to greenhouse soil, punching holes on a small plastic arched shed for ventilation, punching 3 circular holes with the diameter of 1cm per square meter on average, and then gradually enlarging the circular holes.
Compared with the prior art, the sugar-free cultivation strong seedling transplanting method for the detoxified seedlings of the sweet potatoes has the following beneficial effects:
1. selection of the matrix: vermiculite with good air permeability, good water retention performance and less pollution sources is selected as a matrix, sterilization is not needed, the box packing application is directly carried out, the transplanted tissue culture seedlings are free of pollution, the transplanting survival rate is more than 99%, the plants grow well, and new roots grow in 5 days. The survival rate of the tissue culture seedlings is about 79.26-93.04% by taking sand, nutrient soil and other substances as substrates, and the bacterial (mould) infection is more than 10.61%.
2. Screening nutrient solution: 2g/L of calcium ammonium nitrate and 170mg/L of monopotassium phosphate are selected to replace MS to be used as nutrient solution for irrigation, nutrition required by growth of tissue culture seedlings is met, the procedure is simplified, and the production cost is saved.
3. Vermiculite was chosen for substrate moisture: 670 parts of nutrient solution (g: ml): 800 maintains the permeability of the matrix, is convenient for the nutrient absorption and the respiration of the root system of the tissue culture seedling, and has the best growth and rooting of the tissue culture seedling.
4.CO
2And (3) dynamic adjustment: according to the physiological requirements of different growth stages of sweet potato tissue culture seedlings, through CO
2The controller sets the highest value of 1500PPm and the lowest value of 1000PPm, and automatically controls the air release of the air bottle. Start of CO supply on day 5
2300-600 ml/min, and supplying CO at the 10 th day
22000ml/min, and the air supply quantity at different frequencies ensures the normal photosynthesis, nutrient accumulation and root growth and development of the tissue culture seedlings.
5. And (3) illumination control: the light is not applied for the first 2 days, and the light intensity is 80umol/m from the 3 rd day
2S, light intensity of 160umol/m was given at the beginning of day 8
2And/s, illuminating for 12 hours every day to fully ensure the photosynthetic intensity requirement of the tissue culture seedlings.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a diagram showing the growth of jin sweet potato No. 3 in the seedling hardening transition stage in the culture method of example 1.
FIG. 2 is a diagram showing the growth of jin sweet potato No. 9 in the seedling hardening transition stage in the culture method of example 1.
As can be seen from the figure 1 and the figure 2, the leaves of the plants of the two varieties in the seedling hardening transition period are enlarged and are relatively upright, the leaves are dark green, and the plants grow robustly.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention will be described in detail below. It is to be understood that the described embodiments are merely exemplary of the invention, and not restrictive of the full scope of the invention. All other embodiments, which can be derived by a person skilled in the art from the examples given herein without any inventive step, are within the scope of the present invention.
In the present invention, "detoxified seedling" refers to a plant during the cultivation period in a sugar-containing culture flask, "transplanted seedling" and "tissue-cultured seedling" both refer to a plant taken out of a culture box, and "surviving seedling" refers to a plant after 15 days of transplantation in a greenhouse.
Example 1
The embodiment provides a sugar-free culture strong seedling transplanting method for detoxified sweet potato seedlings, which comprises the following steps:
(1) carrying out sweet potato virus-free tissue culture in a test tube, proliferating virus-free seedlings, selecting virus-free seedlings growing for 45 days, cutting old roots, cutting the virus-free seedlings into 4cm stem sections, transplanting the stem sections into a sugar-free culture box filled with vermiculite, pouring 800mL of nutrient solution into each box filled with 670g of vermiculite, transplanting 70 seedlings into each box, covering a box cover, and sealing a gap between the box cover and the box body by using a sealing film; the nutrient solution contains 2g/L of calcium ammonium nitrate and 170mg/LKH
2PO
4The balance being deionized water;
(2) transplanting, placing on a culture shelf in a closed room, and adopting CO
2The controller introduces CO into the culture box
2，CO
2CO set by controller
2The highest concentration is 1500PPm, the lowest concentration is 1000PPm, the ventilation hole of the culture box is opened, and CO is introduced along with the increase of the culture period
2The amount of (a) gradually becomes larger;
(3) the culture period is not irradiated with light for 2 days, and the irradiation intensity is 80umol/m from day 3
2S, light intensity of 160umol/m was given at the beginning of day 8
2S, light 12 hours per day;
(4) supplementing nutrient solution in time according to the growth condition of the detoxified seedlings;
(5) after culturing for 25 days in the culture box, opening the box cover, performing seedling hardening transition under natural conditions, spraying water on the 3 rd day according to the dryness and humidity of vermiculite, and moving to a greenhouse after 4 days;
(6) taking out the tissue culture seedlings from the culture box and transplanting the tissue culture seedlings into greenhouse soil, wherein the soil humidity in the greenhouse is 13-14%, and the environment humidity is more than 75%, after the tissue culture seedlings are transplanted into the greenhouse soil, building a plastic small arched shed, when the temperature in the greenhouse is higher than 32 ℃, needing to cover a sunshade net covering 50% of the sunshade net, transplanting the tissue culture seedlings to the 7 th day after the greenhouse soil, punching holes on the plastic small arched shed for ventilation, punching 3 round holes with the diameter of 1cm per square meter on average, and then gradually enlarging the round holes.
In this example, CO was not carried out for the first 4 days of the culture period
2Gas supply, start CO on day 5
2The gas supply amount was 300ml/min, and CO began on day 10
2The gas supply amount is 2000ml/min, and CO
2The air supply time of (a) is consistent with the time of giving light.
In this example, CO was introduced into the culture box
2Can use CO
2The gas cylinder is used as a gas source. The light irradiation is not carried out for 2 days before the culture period, which is favorable for the rapid seedling recovery of the virus-free seedlings. The light intensity was 80umol/m from day 3
2S, light intensity of 160umol/m was given at the beginning of day 8
2And/s, the illumination is carried out for 12 hours every day, so that the normal photosynthesis of the plantlets can be ensured. The CO is
2The controller is a device conventionally used in the art. In particular, it can be carried out by CO
2The split-flow column valve on the controller is used for adjusting the ventilation volume in the culture box.
In the invention, the illumination can adopt lighting equipment. In this embodiment, the lighting device is a lamp tube, and when two lamps are turned on, the illumination intensity is 80umol/m
2S; when four lamps are turned on, the illumination intensity is 160umol/m
2/s。
Example 2
The embodiment provides a sugar-free culture strong seedling transplanting method for detoxified sweet potato seedlings, which comprises the following steps:
(1) culturing detoxified tissue of sweet potato in test tube, proliferating the detoxified seedling, and selecting 50 days old detoxified seedlingCutting old roots of seedlings, shearing the old roots into 4cm stem sections, transplanting the cut stems into sugar-free culture boxes filled with vermiculite, pouring 800mL of nutrient solution into each box, transplanting 70 seedlings into each box, covering a box cover, and sealing gaps between the box cover and the box body by using sealing films; the nutrient solution contains 2g/L of calcium ammonium nitrate and 170mg/LKH
2PO
4The balance being deionized water;
(2) transplanting, placing on a culture shelf in a closed room, and adopting CO
2The controller introduces CO into the culture box
2，CO
2CO set by controller
2The highest concentration is 1500PPm, the lowest concentration is 1000PPm, the ventilation hole of the culture box is opened, and CO is introduced along with the increase of the culture period
2The amount of (a) gradually becomes larger;
(3) the culture period is not irradiated with light for 2 days, and the irradiation intensity is 80umol/m from day 3
2S, light intensity of 160umol/m was given at the beginning of day 8
2S, light 12 hours per day;
(4) supplementing nutrient solution in time according to the growth condition of the detoxified seedlings;
(5) after culturing for 25 days in the culture box, opening the box cover, performing seedling hardening transition under natural conditions, spraying water on the 3 rd day according to the dryness and humidity of vermiculite, and moving to a greenhouse after 4 days;
(6) taking out the tissue culture seedlings from the culture box and transplanting the tissue culture seedlings into greenhouse soil, wherein the soil humidity in the greenhouse is 13-14%, and the environment humidity is more than 75%, after the tissue culture seedlings are transplanted into the greenhouse soil, building a plastic small arched shed, when the temperature in the greenhouse is higher than 32 ℃, needing to cover a sunshade net covering 50% of the sunshade net, transplanting the tissue culture seedlings into the greenhouse soil on the 6 th day, punching the plastic small arched shed for ventilation, punching 3 round holes with the diameter of 1cm per square meter on average, and then gradually enlarging the round holes.
In this example, CO was not carried out for the first 4 days of the culture period
2Gas supply, start CO on day 5
2The gas supply amount was 600ml/min, and CO began on day 10
2The gas supply amount is 2000ml/min, and CO
2The air supply time of (a) is consistent with the time of giving light.
Example 3
The embodiment provides a sugar-free culture strong seedling transplanting method for detoxified sweet potato seedlings, which comprises the following steps:
(1) carrying out sweet potato virus-free tissue culture in a test tube, proliferating virus-free seedlings, selecting virus-free seedlings growing for 55 days, cutting old roots, cutting the virus-free seedlings into 4cm stem sections, transplanting the stem sections into a sugar-free culture box filled with vermiculite, pouring 800mL of nutrient solution into each box filled with 670g of vermiculite, transplanting 70 seedlings into each box, covering a box cover, and sealing a gap between the box cover and the box body by using a sealing film; the nutrient solution contains 2g/L of calcium ammonium nitrate and 170mg/LKH
2PO
4The balance being deionized water;
(2) transplanting, placing on a culture shelf in a closed room, and adopting CO
2The controller introduces CO into the culture box
2，CO
2CO set by controller
2The highest concentration is 1500PPm, the lowest concentration is 1000PPm, the ventilation hole of the culture box is opened, and CO is introduced along with the increase of the culture period
2The amount of (a) gradually becomes larger;
(3) the culture period is not irradiated with light for 2 days, and the irradiation intensity is 80umol/m from day 3
2S, light intensity of 160umol/m was given at the beginning of day 8
2S, light 12 hours per day;
(4) supplementing nutrient solution in time according to the growth condition of the detoxified seedlings;
(5) after culturing for 25 days in the culture box, opening the box cover, performing seedling hardening transition under natural conditions, spraying water on the 3 rd day according to the dryness and humidity of vermiculite, and moving to a greenhouse after 4 days;
(6) taking out the tissue culture seedlings from the culture box and transplanting the tissue culture seedlings into greenhouse soil, wherein the soil humidity in the greenhouse is 13-14%, and the environment humidity is more than 75%, after the tissue culture seedlings are transplanted into the greenhouse soil, building a plastic small arched shed, when the temperature in the greenhouse is higher than 32 ℃, needing to cover a sunshade net covering 50% of the sunshade net, transplanting the tissue culture seedlings into the greenhouse soil on the 5 th day, punching holes on the plastic small arched shed for ventilation, punching 3 round holes with the diameter of 1cm per square meter on average, and then gradually enlarging the round holes.
In this example, CO was not carried out for the first 4 days of the culture period
2Gas supply, start CO on day 5
2The gas supply rate was 450ml/min, and CO began on day 10
2The gas supply amount is 2000ml/min, and CO
2The air supply time of (a) is consistent with the time of giving light.
Example 4: effect of matrix on sugar-free culture
The jin sweet potato No. 3 and the jin sweet potato No. 9 were selected and cultured by the method described in example 1, respectively, to prepare vermiculite groups. Similarly, the substrate was changed to sand as a sand group, and the culture was carried out using the method described in example 1, using the jin sweet potato variety No. 3 and the jin sweet potato variety No. 9. Each set of experiments was repeated three times and the average was taken to obtain the following data.
TABLE 1 Effect of matrix on sugarless culture
Example 5: growth influence of nutrient solution on detoxified seedling
The Jinshan sweet potato No. 3 was cultured by the method described in examples, and used as an experimental group. Similarly, the method described in example 1 was used to select jin sweet potato No. 3 for cultivation, but the nutrient solution was changed to MS as a control. Each set of experiments was repeated three times and the average was taken to obtain the following data.
TABLE 2 Effect of nutrient solutions on the growth of tissue culture seedlings
As can be seen from table 2, the cost of the nutrient solution provided by the present invention is much lower than that of the conventional MS nutrient solution. Moreover, based on the consideration of growth potential, rooting amount and cost, 2g/L of calcium ammonium nitrate and 170mg/L of KH in nutrient solution are preferably adopted
2PO
4。
Example 6: the growth influence of the added nutrient solution on the virus-free seedlings
The Jinyao No. 9 was cultured by the method described in the examples, but the ratio of the nutrient solution to vermiculite was adjusted accordingly as shown in Table 3. Each set of experiments was repeated three times and the average was taken to obtain the following data.
TABLE 3 influence of vermiculite humidity on growth and transplant survival rate of detoxified seedlings
Note: + means better growth; + good growth ++ robust growth
As can be seen from table 3, when the ratio of vermiculite to nutrient solution is 670: when 800 hours, the virus-free seedlings grow well, and the transplanting survival rate is high.
Example 7: growth influence of culture mode on virus-free seedlings
The variety jin sweet potato No. 3 and the variety jin sweet potato No. 9 were selected and cultured by the method described in example 1. Adopting the prior conventional sugary culture mode to select No. 3 and No. 9 Jin sweet potatoes for respective culture. Each set of experiments was repeated three times and the average was taken to obtain the following data.
TABLE 4 influence of different culture methods on the growth of tissue culture seedlings of Ipomoea batatas
As can be seen from table 4: both culture methods yielded new roots after 8 days of culture. The leaves of the sugar-free cultured plants are investigated to be enlarged, more upright, dark green in leaf color and strong in growth after 25 days of culture; transplanting for 25 days, investigating the leaves of the plants cultured with sugar, which are tiny, yellow and withered. The plant heights of No. 3 Jinsweet potato and No. 9 Jinsweet potato cultured in a sugar-free way are 0.477cm and 0.882cm higher than those of the No. 9 Jinsweet potato cultured in a sugar way, and the difference is obvious; the number of roots is increased by 2.04 and 2.32, and the difference is obvious; the root length difference is not significant; the fresh weight of each plant is averagely increased by 2613.9mg and 2654.7mg, and the difference is obvious; the average dry weight increases 265.3mg and 258mg, and the difference reaches a significant level. The pollution rate of sugar-free culture is 0, and the pollution rate of sugar-containing culture is 15.12 percent and 10.07 percent. The sugar-free cultured plants are directly transplanted to a greenhouse for planting because of strong growth, thereby shortening the culture period and the culture time and reducing the production cost.
The above description is only for the specific embodiments of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present invention, and all the changes or substitutions should be covered within the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the appended claims.
Claims (7)
1. A sugar-free culture strong seedling transplanting method for detoxified seedlings of sweet potatoes is characterized by comprising the following steps:
(1) carrying out sweet potato virus-free tissue culture in a test tube, proliferating virus-free seedlings, selecting virus-free seedlings growing for 45-55 days, cutting old roots, shearing the virus-free seedlings into 4cm stem sections, transplanting the stem sections into a sugar-free culture box filled with vermiculite, pouring 800mL of nutrient solution into each box filled with 670g of vermiculite, transplanting 70 seedlings into each box, covering a box cover, and sealing a gap between the box cover and the box body by using a sealing film; the nutrient solution contains 2g/L of calcium ammonium nitrate and 170mg/LKH
2PO
4The balance being deionized water;
(2) transplanting, placing on a culture shelf in a closed room, introducing CO into the culture box
2Opening the ventilation hole of the culture box, and introducing CO along with the growth of the culture period
2The amount of (a) gradually becomes larger;
(3) the culture period is not irradiated with light for 2 days, and the irradiation intensity is 80umol/m from day 3
2S, light intensity of 160umol/m was given at the beginning of day 8
2S, light 12 hours per day;
(4) supplementing nutrient solution in time according to the growth condition of the detoxified seedlings;
(5) after culturing in the culture box for 25 days, the box cover is opened and the illumination intensity is 80umol/m
2Carrying out seedling hardening transition under natural conditions, spraying water according to the dryness and the humidity of vermiculite on day 3, and moving to a greenhouse outside day 4;
(6) taking out the tissue culture seedlings from the culture box and transplanting the tissue culture seedlings into greenhouse soil.
2. According to the claimsThe sugar-free culture strong seedling transplanting method for the detoxified seedlings of the sweet potatoes in the step (1) is characterized in that in the step (2), CO in a closed room is adopted
2The maximum concentration is 1500PPm and the minimum 1000 PPm.
3. The sugar-free cultivation strong seedling transplanting method for the detoxified seedlings of sweet potatoes as claimed in claim 2, wherein in the step (2), CO is not added for the first 4 days of the cultivation period
2Gas supply, start CO on day 5
2The amount of supplied air is 300 to 600ml/min, and CO starts on day 10
2The gas supply amount is 2000ml/min, and CO
2The air supply time of (a) is consistent with the time of giving light.
4. The sugar-free cultivation strong seedling transplanting method for the detoxified seedlings of sweet potatoes as claimed in claim 1, wherein in the step (6), the soil humidity in a greenhouse is 13-14%, and the environmental humidity is more than 75%.
5. The sugar-free culture strong seedling transplanting method for the detoxified seedlings of the sweet potatoes as claimed in claim 1, wherein in the step (6), a small plastic arched shed is constructed after the tissue culture seedlings are transplanted to greenhouse soil.
6. The sugar-free cultivation strong seedling transplanting method for the detoxified seedlings of sweet potatoes as claimed in claim 5, wherein when the temperature in the greenhouse is higher than 32 ℃, a sunshade net with 50% of shading density needs to be covered.
7. The sugar-free cultivation strong seedling transplanting method for the detoxified seedlings of the sweet potatoes as claimed in claim 5 or 6, wherein holes are punched on a small plastic arched shed for ventilation 5-7 days after the seedlings are transplanted to the greenhouse soil, 3 circular holes with the diameter of 1cm are punched on the average per square meter, and then the circular holes are gradually enlarged.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111316900A (en) * | 2020-03-26 | 2020-06-23 | 江苏徐淮地区徐州农业科学研究所(江苏徐州甘薯研究中心) | Method for improving transplanting survival rate of virus-free seedlings of purple-meat sweet potatoes |
| CN111869567A (en) * | 2020-08-12 | 2020-11-03 | 四川省农业科学院生物技术核技术研究所 | Sweet potato seedling propagation method |
| CN115486360A (en) * | 2022-08-29 | 2022-12-20 | 甘肃勇馨科技农业有限公司 | Novel sugar-free efficient rooting culture and transplanting method for detoxified ginseng fruit seedlings |
| CN116349602A (en) * | 2023-04-17 | 2023-06-30 | 河南科技大学 | Sweet potato detoxification tissue culture seedling carbon dioxide sugar-free domestication method |
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| CN115486360A (en) * | 2022-08-29 | 2022-12-20 | 甘肃勇馨科技农业有限公司 | Novel sugar-free efficient rooting culture and transplanting method for detoxified ginseng fruit seedlings |
| CN116349602A (en) * | 2023-04-17 | 2023-06-30 | 河南科技大学 | Sweet potato detoxification tissue culture seedling carbon dioxide sugar-free domestication method |
| CN116349602B (en) * | 2023-04-17 | 2024-04-12 | 河南科技大学 | Sweet potato detoxification tissue culture seedling carbon dioxide sugar-free domestication method |
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