CN109220269A - A kind of Magnolia wufengensis softwood cutting propagation method based on optimal crown canopy time - Google Patents
A kind of Magnolia wufengensis softwood cutting propagation method based on optimal crown canopy time Download PDFInfo
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- 238000005520 cutting process Methods 0.000 title claims abstract description 117
- 241000730180 Magnolia wufengensis Species 0.000 title claims abstract description 45
- 238000000034 method Methods 0.000 title claims abstract description 20
- 239000011122 softwood Substances 0.000 title claims abstract description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 18
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 17
- 238000002360 preparation method Methods 0.000 claims abstract description 9
- 239000007921 spray Substances 0.000 claims abstract description 9
- 239000002689 soil Substances 0.000 claims abstract description 7
- 239000011159 matrix material Substances 0.000 claims description 18
- 238000004659 sterilization and disinfection Methods 0.000 claims description 12
- 241000607479 Yersinia pestis Species 0.000 claims description 9
- 230000006378 damage Effects 0.000 claims description 9
- 201000010099 disease Diseases 0.000 claims description 9
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 9
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 claims description 8
- 238000009825 accumulation Methods 0.000 claims description 8
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 claims description 8
- 239000006013 carbendazim Substances 0.000 claims description 8
- 239000007788 liquid Substances 0.000 claims description 8
- 239000000126 substance Substances 0.000 claims description 8
- 241000196324 Embryophyta Species 0.000 claims description 6
- 239000000645 desinfectant Substances 0.000 claims description 6
- 235000008935 nutritious Nutrition 0.000 claims description 5
- 229930192334 Auxin Natural products 0.000 claims description 3
- 238000005299 abrasion Methods 0.000 claims description 3
- 239000002363 auxin Substances 0.000 claims description 3
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 claims description 3
- 239000012528 membrane Substances 0.000 claims description 3
- 235000016709 nutrition Nutrition 0.000 claims description 3
- 230000035764 nutrition Effects 0.000 claims description 3
- 239000004576 sand Substances 0.000 claims description 3
- 235000015097 nutrients Nutrition 0.000 claims 1
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- 240000005819 Magnolia denudata Species 0.000 description 4
- 235000016094 Magnolia denudata Nutrition 0.000 description 4
- 238000009395 breeding Methods 0.000 description 4
- DEDOPGXGGQYYMW-UHFFFAOYSA-N molinate Chemical compound CCSC(=O)N1CCCCCC1 DEDOPGXGGQYYMW-UHFFFAOYSA-N 0.000 description 4
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- 235000003255 Carthamus tinctorius Nutrition 0.000 description 1
- 244000020518 Carthamus tinctorius Species 0.000 description 1
- 241000218377 Magnoliaceae Species 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- Life Sciences & Earth Sciences (AREA)
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- Environmental Sciences (AREA)
- Developmental Biology & Embryology (AREA)
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Abstract
The present invention provides a kind of Magnolia wufengensis softwood cutting propagation method based on optimal crown canopy time, comprising the following steps: the preparation before 1. cuttages;2. the acquisition and preparation of cuttings;3. cuttings sterilizes;4. cuttings is impregnated;5. cuttage;6. being managed after cuttage.The present invention is using the raw Magnolia wufengensis seedling of 5-6 as scion-seed tree, and the current year raw semi-lignified branch for choosing tree crown middle layer is raw material, and cuttings base portion is impregnated before cuttage with root-growing agent.Shaded using shading net, and interval sprays water mist, can effectively keep cuttage canopy interior and the temperature and humidity of plugging soil, promote cuttage rooting.The invention has the advantages that improving the rooting rate of Magnolia wufengensis shoot cutting, the low technical barrier of Magnolia wufengensis cutting plantation has been captured, the long deficiency of large nursery stocks cultivation period has been overcome, is conducive to the fast-propagation of Magnolia wufengensis.
Description
Technical field
The present invention relates to a kind of reproduction technique of trees, specifically a kind of Magnolia wufengensis based on optimal crown canopy time is tender
Branch cuttage breeding method.
Background technique
Magnolia wufengensis (Magnolia wufengensis L.Y.Ma et L.R.Wang) is Beijing Forestry University Ma Lvyi
The Magnoliacea plant novel species found when Plant Resource of Magnoliaceae investigation is carried out in Hubei Wu Feng within professor et al. 2004.Before this
In formally recording, yulan only has white, purple and single side pink, and complete red yulan still belongs to the first time at home inside and outside discovery,
Only five peaks are exclusive.
Magnolia wufengensis wild resource is only distributed in Wufeng County, and Magnolia wufengensis hayashishita seedling is dilute under natural conditions
It is few, difficulty is updated, percentage of seedgermination and storage rate are all lower, and conventional seed planting mode of reproduction is not easy seedling, therefore is badly in need of to safflower
Yulan carries out the research of vegetative propagation, this not only contributes to industrial seedling rearing, improves Magnolia wufengensis reproductive efficiency, and reduction is produced into
This, but also the clone of merit can be filtered out, needed for meeting production, watching, to Magnolia wufengensis scientific research, application value
Exploitation be of great significance.Magnolia wufengensis breeding common at this stage mostly uses grafting and Mode of sowing and seedling propagation, and cuttage is numerous
It grows since rooting rate is lower, one is directly subordinate to the problem not being hacked.
Inventor passes through a large amount of Cutting test, and discovery cuttage is the feasible method of Magnolia wufengensis fast-propagation, and summarizes
A set of more complete Magnolia wufengensis shoot cutting technical system is gone out.
Summary of the invention
Goal of the invention: the deficiency in order to make up existing Technique on Cuttage Propagation, the purpose of the present invention is to provide one kind to take root
The higher Magnolia wufengensis softwood cutting propagation method of rate.This method is easy to operate practical, easy to spread, cutting plantation can be made to reach
To 72%.
Technical solution: in order to solve the above technical problems, technical solution provided by the invention is, choose it is healthy and strong, grow fine,
The raw Magnolia wufengensis seedling of the 5-6 of no disease and pests harm is that scion-seed tree intercepts cuttings, and late May, seedling tree crown middle layer is worked as
Year, semi-lignified state was presented in raw branch, and nutritious substances accumulation is suitable for that blade state is good, and leaf bud is full, can adopt at this time
Collect fringe processed, carries out cuttage.Specific steps include:
A. the preparation before cuttage: the slotting machine of long 6m, width 1.5m, high 15cm are puzzled in advance, and builds 1m high on slotting machine top
Arched shed, along ceiling with hose be laid with self-timing spray equipment, select river sand as cutting medium, before cuttage to matrix into
Row adequate disinfection;
B. the acquisition and preparation of cuttings: the acquisition of cuttings is preferably selected the cloudy day or cloudy weather carries out, and chooses healthy and strong, long
Gesture is good, the no disease and pests harm raw Magnolia wufengensis seedling of 5-6 is scion-seed tree, acquisition branch fringe, and each cuttings is about 12-
15cm retains half leaf of cuttings top 2-3 piece and 2-3 full buds, and upper lower cut is truncation, and guarantees that notch is smooth, is prevented
Incision is rotted;
C. the disinfection of cuttings: the cuttings sheared is totally immersed into cuttings disinfectant adequately disinfected;
Cuttings Cheng Bales after disinfection: being put the morphology upper and lower side for neatly paying attention to cuttings to be distinguished by the D. immersion of cuttings,
Cuttings base portion is immersed in the bucket for filling root-growing agent, keeps cuttings upright, and guarantee that every stem cutting fringe base portion can be dipped into and take root
Agent, while noticing that the blade of cuttings cannot be stained with root-growing agent;
E. cuttage: shakeouing matrix before cuttage, sprinkles profoundly water, and it is soft wet to keep matrix, and in advance with slightly than diameter
Wide punch equally spacedly prepares jack on slotting machine, to prevent abrasion cuttings base portion, hole depth 4-6cm, when cuttage it is noted that
The morphology upper and lower side for distinguishing cuttings, prevents from inserting, while keeping the direction of blade consistent, and cuttage is deep-controlled in 4-6cm model
In enclosing, it is compacted matrix with hand after cuttage, makes plugging soil and cuttings base portion close contact, and drenched matrix with water immediately;
F. manage after cuttage: being sterilized 1 time after cuttage every 7d, inspect periodically on slotting machine whether with weeds and pest and disease damage simultaneously
It takes corresponding measure to prevent and control, spray time interval is rationally set according to weather condition, guarantee cuttage seedbed not water shortage
Not ponding, blade surface forms water membrane, while carrying out shade measure, and at 19-29 DEG C, air is opposite to be contained control temperature of shed
Water rate is 85%-100%, and the soil moisture is within the scope of 21-29 DEG C.
In order to improve rooting rate, 0.2% liquor potassic permanganate is selected in the nursery bed disinfection agent, and is needed at least in cuttage
First 2-3 days adequately disinfected to matrix, and through solar exposure two days.
The cuttings is prepared by the current year raw semi-lignified branch in tree crown middle layer.Late May, under tree crown upper layer and tree crown
Though layer newly germinates branch out containing more auxin substance, its degree of lignification is low compared with middle layer, and branch is excessively young tender,
It easily rots when cuttage, and its nutritious substances accumulation is few, it is difficult to provide foot to cuttage root-taking period longer Magnolia wufengensis cuttings
Enough nutrition supplies.
The cuttings disinfectant is 800 times of liquid carbendazim, and need to impregnate 1min in 800 times of liquid of carbendazim.
The root-growing agent is the NAA solution of 1000mg/L.
The blade of the cuttings need to be trimmed to original half, that is, stay half leaf, since Magnolia wufengensis blade is larger, and
During cuttage, cuttings itself not yet forms good water conservation water suction mechanism, thus blade need to be done it is some trimming with reduce due to
Transpiration and the loss of water generated.
Soaking time of the cuttings in root-growing agent need to be 2h, and soaking time is too short, and root-growing agent effect is difficult to play,
Soaking time is too long, is easy that cuttings is made to rot, therefore need to get hold of soaking time to guarantee the best performance facilitation of root-growing agent.
The disinfectant used when managing after the cuttage is 800 times of liquid of carbendazim, after cuttage, is needed periodically to cuttings and seedling
Bed carries out disinfection, and prevents the generation of pest and disease damage.
The utility model has the advantages that the rooting rate of Magnolia wufengensis softwood cutting propagation can be significantly improved, so that rooting rate reaches 72%,
The low technical barrier of Magnolia wufengensis cutting plantation has been captured, Magnolia wufengensis breeding process has been accelerated, is the big of its germ plasm resource
Amount expansion is numerous to provide technological guidance.
Detailed description of the invention
Above-mentioned and/or additional aspect of the invention and advantage will become from the description of the embodiment in conjunction with the following figures
Obviously and it is readily appreciated that, in which:
Fig. 1 is the rooting effect scoring exemplary diagram of the embodiment of the present invention;
Specific embodiment
Below with reference to example, the present invention is described in further detail.It should be pointed out that the present embodiment is to of the invention
Principle and feature are described, and the given examples are served only to explain the present invention, overcome the long technology of large nursery stocks cultivation period
Deficiency is conducive to the breeding process for accelerating Magnolia wufengensis, provides guidance for the fast-propagation of its germ plasm resource.
Embodiment 1.
For one kind based on optimal crown canopy time Magnolia wufengensis softwood cutting propagation method, specific operating procedure is as follows:
Preparation before cuttage: the slotting machine of long 6m, width 1.5m, high 15cm are puzzled in advance, and builds 1m's high on slotting machine top
Arched shed is laid with self-timing spray equipment along ceiling hose.Select river sand as cutting medium, before cuttage 2-3 days with dense
Degree carries out adequate disinfection to matrix for 0.2% liquor potassic permanganate;And squirt matrix, it shakeouts, it is spare;
The acquisition and preparation of cuttings: on May 23rd, 2018, cloudy day.It chooses stalwartness, grow fine, lifes in 6 years of no disease and pests harm
Magnolia wufengensis seedling acquires the current year raw semi-lignified branch in tree crown middle layer, intercepts branch middle and upper part system as scion-seed tree
Cuttings, every stem cutting spike length 15cm stay 2 half leaves, and stay 2-3 full buds, and upper lower cut is truncation, guarantee that notch is smooth,
Prevent incision from rotting;
Cuttings disinfection: preparing 800 times of liquid of carbendazim, and the cuttings made is totally immersed into wherein, 1min is impregnated;
Cuttings is impregnated: the cuttings Cheng Bales after disinfection being put to the morphology upper and lower side for neatly paying attention to distinguishing cuttings, Jiang Cheng Bales
The base portion of cuttings immerses in 1000mg/L NAA solution, guarantees that every stem cutting fringe base portion can be dipped into solution, while paying attention to cuttings
Blade cannot be stained with root-growing agent;
Cuttage: shakeouing matrix before cuttage, sprinkles profoundly water, and it is soft wet to keep matrix, and in advance with more slightly wider than diameter
Punch jack is equally spacedly prepared on slotting machine, prevent abrasion cuttings base portion, hole depth 5cm.The court of blade is kept when cuttage
To consistent.Cuttage depth is 5cm, is compacted matrix with hand after cuttage, makes plugging soil and cuttings base portion close contact, and drenched immediately with water
Saturating matrix;
Managed after cuttage: every 7d 800 times of carbendazim liquid disinfectant 1 time after cuttage, inspect periodically on slotting machine whether with
Weeds and pest and disease damage simultaneously take corresponding measure to prevent and control, and spray time interval is rationally arranged according to weather condition, guarantees
Ponding, blade surface do not form water membrane to water shortage yet in cuttage seedbed.Shade measure is carried out simultaneously.Control temperature of shed exists
19-29 DEG C, air relative moisture content is 85%-100%, and the soil moisture is within the scope of 21-29 DEG C.
Actual effect: observation spray is taken root situation after cultivating 80 days, and cutting plantation reaches 72%.
Embodiment 2.
1 experimental design: on May 23rd, 2018, in the Forest Cultivation subject greenhouse of Beijing Forestry University vulture peak proving ground
Cuttage canopy has been built to be tested.Single factor test completely randomized experiment more different cuttings acquisitions position is designed to Magnolia wufengensis cuttage
The influence taken root.Using the NAA solution of 1000mg/L as root-growing agent, respectively from tree crown upper layer, tree crown middle layer and tree crown lower layer
Acquisition current year, raw semi-lignified branch prepared cuttings, and totally 3 processing, every 30 plants of cuttings of processing cuttage are repeated 3 times.
The acquisition position for removing cuttings is different outer, other basic operations during cuttage with the phase in claims
Together, it does not repeat herein.
2 take root situation statistics: the growing state of continuous observation cuttings base portion after cuttage, statistics is taken root feelings after cuttage 80 days
Condition counts the rooting rate of each processing, the quantity of taking root of every stem cutting fringe, average root long, average root diameter, rooting effect scoring rate.
Length is accurate to 0.01cm, and diameter is accurate to 0.01mm.
The rooting effect of cuttings scores: cuttage root system being divided into 3 grades, is assessed as 1,2,3 points respectively.By cuttings base portion
Level-one radical less than 3, it is more single underdeveloped without second level root, root system, be assessed as 1 point;It is small that cuttings base portion level-one radical is greater than 3
In 7, have second level root system, Root Distribution more uniformly, thickness it is moderate, be assessed as 2 points;Cuttings base portion root system development is more complete
Kind, lateral root series is more than or equal to 7, has good fixed function to the cutting medium around root system, is assessed as 3 points.3 when scoring
People carries out comprehensive score to root system together, finally calculates final score value of three's mean value as each cuttings.
Wherein: N1For the quantity for the cuttage seeding that root system scoring is 1 point;N2For the quantity for the cuttage seeding that root system scoring is 2 points;
N3For the quantity for the cuttage seeding that root system scoring is 3 points.The example that scores is as shown in Figure 1.
3 interpretations of result
Table 1 is the variance analysis and the result of multiple comparisons of Magnolia wufengensis cutting plantation and cuttings acquisition position.As it can be seen that not
There are extremely significant differences for the cuttings cuttage rooting rate acquired with tree crown level.Acquire influence size of the position to cutting plantation according to
It is secondary are as follows: middle layer, upper layer, lower layer.Speculate that reason may are as follows: when late May, tree crown upper layer and tree crown lower layer newly germinate branch out
Though its degree of lignification is low compared with middle layer containing more auxin substance, branch is excessively young tender, easily rots when cuttage,
And its nutritious substances accumulation is few, it is difficult to provide enough nutrition supplies to cuttage root-taking period longer Magnolia wufengensis cuttings.And
The cuttings of tree crown middle layer acquisition, degree of lignification are suitable for that the nutritious substances accumulation upper layer of blade state and cuttings and lower layer are more
For maturation, thus it is easier to take root.
Influence of the 1 different acquisition position of table to Magnolia wufengensis shoot cutting rooting rate
Table 2 summarizes 4 morphological indexs of cuttage root-taking.As shown in Table 2, the cuttings of tree crown middle layer acquisition, cuttage are raw
Root par, average root long, average root diameter and rooting effect scoring rate are to behave oneself best in 3 acquisition positions,
Average root long is even more that there are extremely significant differences with tree crown upper layer, lower layer.As it can be seen that cuttings of the acquisition from tree crown middle layer, whole raw
Root effect is preferable, wherein the most obvious to the promotion of average root long.
Influence of the 2 different acquisition position of table to each Root morphology index of Magnolia wufengensis shoot cutting
5 indexs of all processing are carried out principal component analysis, and (all data have used SPSS to carry out z-score standard
Change), variance and variance the accumulation contribution rate of each ingredient are shown in Table 3, SPSS and are extracted the principal component that 1 characteristic value is greater than 1, are extracted
Principal component variance accumulation contribution rate be 90.35%.
The variance and variance of each ingredient of table 3 accumulate contribution rate
It arranges two principal component expression formulas and comprehensive score formula is as follows:
C=0.988X1+0.937X2+0.999X3+0.953X4+0.870X5(formula 3)
Wherein: C indicates principal component scores;X1To X5Cuttage is respectively indicated to be averaged rooting rate, averagely take root quantity, average root
This 5 indexs of length, average root diameter, rooting effect scoring rate.
5 index values of each processing are substituted into formula 3, the score and ranking for arranging each processing are shown in Table 4.It can by overall ranking
Know, in 3 acquisition positions, the cuttings cuttage acquired using tree crown middle layer, root system comprehensive score highest, rooting efficiency is best.
The principal component scores and comprehensive score that table 4 is respectively handled
4 conclusions
Test result shows that the cuttings acquired using tree crown middle layer carries out cuttage, and rooting efficiency is best, may advantageously facilitate red
Flower yulan spray generates the root system that complete, strong, form is excellent, quality is good.
The above is only the preferred embodiment of the present invention, it is noted that aforesaid way is not construed as to of the invention
Limitation, protection scope of the present invention should be defined by the scope defined by the claims..For the ordinary skill of the art
For personnel, without departing from the spirit and scope of the present invention, several improvements and modifications can also be made, these improvements and modifications
Also it should be regarded as protection scope of the present invention.
Claims (8)
1. a kind of Magnolia wufengensis softwood cutting propagation method based on optimal crown canopy time, which is characterized in that late May, selection
Stalwartness grows fine, the semi-lignified branch preparation cuttings in the raw Magnolia wufengensis seedling tree crown middle layer the 5-6 of no disease and pests harm.
The branch in tree crown middle layer, degree of lignification is convenient, and nutritious substances accumulation is abundant, and blade state is good, and leaf bud is full, acquisition
The branch at this position prepares cuttings, carries out cuttage, is greatly improved the rooting rate of Magnolia wufengensis shoot cutting.Specific steps packet
It includes:
A. the preparation before cuttage: the slotting machine of long 6m, width 1.5m, high 15cm are puzzled in advance, and builds the arch of 1m high on slotting machine top
Canopy is laid with self-timing spray equipment along ceiling hose, selects river sand as cutting medium, fill before cuttage to matrix
Divide disinfection;
B. the acquisition and preparation of cuttings: the acquisition of cuttings is preferably selected the cloudy day or cloudy weather carries out, and it is good to choose stalwartness, growing way
Good, no disease and pests harm the raw Magnolia wufengensis seedling of 5-6 is scion-seed tree, acquires branch fringe, every stem cutting spike length about 12-
15cm retains half leaf of cuttings top 2-3 piece and 2-3 full buds, and upper lower cut is truncation, should ensure that notch is smooth, is prevented
Only incision is rotted;
C. cuttings sterilizes: the cuttings sheared being totally immersed into cuttings disinfectant adequately disinfected;
D. cuttings is impregnated: the cuttings Cheng Bales after disinfection being put to the morphology upper and lower side for neatly paying attention to cuttings to be distinguished, by cuttings
Base portion immerses in the bucket for filling root-growing agent, keeps cuttings upright, and guarantee that every stem cutting fringe base portion can be dipped into root-growing agent, simultaneously
Notice that the blade of cuttings cannot be stained with root-growing agent;
E. cuttage: shakeouing matrix before cuttage, sprinkles profoundly water, and it is soft wet to keep matrix, and in advance with more slightly wider than diameter
Punch equally spacedly prepares jack on slotting machine, to prevent abrasion cuttings base portion, hole depth 4-6cm, it is noted that distinguishing when cuttage
The morphology upper and lower side of cuttings, prevents from inserting, while keeping the direction of blade consistent, and cuttage is deep-controlled within the scope of 4-6cm,
It is compacted matrix with hand after cuttage, makes plugging soil and cuttings base portion close contact, and is drenched matrix with water immediately;
F. it manages after cuttage: being sterilized 1 time after cuttage every 7d, inspect periodically on slotting machine and whether with weeds and pest and disease damage and to take
Corresponding measure prevention and control, spray time interval is rationally arranged according to weather condition, guarantees that water shortage is not accumulated yet in cuttage seedbed
Water, blade surface form water membrane, while carrying out shade measure, and control temperature of shed is at 19-29 DEG C, air relative moisture content
For 85%-100%, the soil moisture is within the scope of 21-29 DEG C.
2. according to claim 1, the Magnolia wufengensis softwood cutting propagation method, which is characterized in that nursery bed disinfection was selected
0.2% liquor potassic permanganate, and need at least before cuttage 2-3 days it is adequately disinfected to matrix, and through solar exposure two days.
3. according to claim 1, the Magnolia wufengensis softwood cutting propagation method, which is characterized in that acquisition tree crown middle layer
Current year, raw semi-lignified branch prepared cuttings.Late May, though tree crown upper layer and tree crown lower layer newly germinate branch out containing more
Auxin substance, but its degree of lignification is low compared with middle layer, and branch is excessively young tender, and when cuttage easily rots, and its nutrients
Matter accumulation is few, it is difficult to provide enough nutrition supplies to cuttage root-taking period longer Magnolia wufengensis cuttings.
4. according to claim 1, the Magnolia wufengensis softwood cutting propagation method, which is characterized in that the cuttings disinfection
Agent is 800 times of liquid carbendazim, and need to impregnate 1min in 800 times of liquid of carbendazim.
5. according to claim 1, the Magnolia wufengensis softwood cutting propagation method, which is characterized in that the root-growing agent is
NAA 1000mg/L solution.
6. according to claim 1, the Magnolia wufengensis softwood cutting propagation method, which is characterized in that the blade that cuttings is stayed
Need to be cut into original half.
7. according to claim 1, the Magnolia wufengensis softwood cutting propagation method, which is characterized in that cuttings impregnates root-growing agent
Time be 2h.
8. according to claim 1, the Magnolia wufengensis softwood cutting propagation method, which is characterized in that managed after the cuttage
The disinfectant used when reason is 800 times of liquid of carbendazim.
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