CN109172634A - A kind of skin mucosa disinfectant and preparation method thereof for wound healing - Google Patents
A kind of skin mucosa disinfectant and preparation method thereof for wound healing Download PDFInfo
- Publication number
- CN109172634A CN109172634A CN201811370414.8A CN201811370414A CN109172634A CN 109172634 A CN109172634 A CN 109172634A CN 201811370414 A CN201811370414 A CN 201811370414A CN 109172634 A CN109172634 A CN 109172634A
- Authority
- CN
- China
- Prior art keywords
- extracting solution
- time
- solution
- peppermint
- skin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/35—Caprifoliaceae (Honeysuckle family)
- A61K36/355—Lonicera (honeysuckle)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/74—Synthetic polymeric materials
- A61K31/785—Polymers containing nitrogen
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
- A61K36/232—Angelica
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/489—Sophora, e.g. necklacepod or mamani
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/534—Mentha (mint)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0014—Skin, i.e. galenical aspects of topical compositions
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/08—Solutions
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/02—Local antiseptics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/10—Antimycotics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/13—Preparation or pretreatment of starting material involving cleaning, e.g. washing or peeling
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/15—Preparation or pretreatment of starting material involving mechanical treatment, e.g. chopping up, cutting or grinding
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/17—Preparation or pretreatment of starting material involving drying, e.g. sun-drying or wilting
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/37—Extraction at elevated pressure or temperature, e.g. pressurized solvent extraction [PSE], supercritical carbon dioxide extraction or subcritical water extraction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- General Health & Medical Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Medicinal Chemistry (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Chemical & Material Sciences (AREA)
- Epidemiology (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Medical Informatics (AREA)
- Botany (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Communicable Diseases (AREA)
- Oncology (AREA)
- Dermatology (AREA)
- Virology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Inorganic Chemistry (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The skin mucosa disinfectant and preparation method thereof that the present invention relates to a kind of for wound healing, the thimerosal consists of the following compositions: Flos Lonicerae extractive solution 1.0%-8.0%, peppermint extracting solution 1.0%-4.5%, Radix Sophorae Flavescentis extractive liquid 1.0%-6.0%, root of Dahurain angelica extracting solution 1.0%-6.0%, polyhexamethylene guanide (PHMG) 0.08%-0.70%, surplus are low deuterium-oxide, this product without iodine, without alcohol, without aldehyde, without phenol, without chlorine, it is soft non-stimulated, not sensitization;It is environmentally protective, care health.Since the low deuterium-oxide of the base fluid of this product is small-micelle water, then other medicines use is cooperated to can promote Skin Cell activity, so can be shortened the time of union of wounded skin.
Description
Technical field
The present invention relates to a kind of skin mucosa disinfectants for wound healing, belong to the field of Chinese medicines, and in particular to Yi Zhongyong
In the skin mucosa disinfectant and preparation method thereof of wound healing.
Background technique
Harmful microbes bring many diseases to the mankind, seriously endanger the life security of people.For being good for for people
Health, sterilizing become maintenance human life essential important link safely.And it is the most convenient, the most commonly used is various sterilizations
Agent.It common are iodine class, alcohols, aldehydes at present, contain phenols, the disinfectants such as class containing chlorine, these fungicide generally have bigger
Volatile flavor and irritant to skin and mucosa, and most fungicide has certain toxicity, and long-time service will cause
Environmental pollution.
For example, having been reported that iodine class disinfectant can cause skin injury, and it can absorb into blood, makes iodine level liter in serum and urine
Height can cause temporary hypothyroidism, Thyroid Gland Swell, so pregnant woman disables, newborn is used with caution.
Newborn skin is more fragile, and experienced and be exposed to outside air after amniotic fluid to birth out of maternal uterine
Intense environment variation, so being easy by different degrees of damage, to cause other systems disease.Ethyl alcohol is also commonly to disappear
Toxic agent, but Disinfection Effect is general, and irritation and high volatility, can cause to have dry skin, or even cause permanent skin
Damage.Ethyl alcohol absorptivity is high, big to newborn's toxicity, had better not be used for newborn skin disinfection.
It is widely used as the chlorhexidine acetate nineties in last century is clinical at home, it is related bad since nearly 30 years
Reaction report is also more and more.Mainly to skin and mucosa, there are certain irritations and anaphylaxis, cause wound surrounding skin
Generate dermatitis.Simultaneously because being used for a long time, drug resistance is produced, to increase the risk of infection.
Skin is the important organ of human body, have barrier, absorption, feeling, secretion and excretion, body heat regulation, metabolism,
The functions such as immune.Wound easy infection, accelerating wound healing is the optimal path for restoring skin barrier function.Currently, in the market still
It not only not can be used for sterilizing, but also can be with wound healing, while newborn's skin can also be used for without secondary land used safely
Skin infection of umbilicus, red stern, pustular eruption Chinese materia medica preparation.
Due to available without product more of new generation, domestic hospitals clinic still states product in use at present.
In the prior art: Chinese Patent Application No.: CN201310409073.1, a kind of denomination of invention: suppression of sterilizing
Microbial inoculum and skin mucosa disinfectant and preparation method thereof, it is made of the raw material in following weight range: honeysuckle 15-
25kg, bo-chrysanthemum 15-25kg, peppermint 15-25kg, dandelion 15-25kg, folium isatidis 3-8kg, climbing groundsel 3-8kg, tribulus terrestris 3-
8kg, acetic acid chlorhexidine, 0.9-0.12kg, cosolvent 0.1-0.3kg, ethyl alcohol 0.1-0.3kg.Preparation method: (1) will be described heavy
Honeysuckle, bo-chrysanthemum, peppermint, dandelion, folium isatidis, climbing groundsel, the tribulus terrestris of amount are put into molten device of boiling medicine and decoct secondary, first time
Add 8 times of water amounts, impregnate 30 minutes, 80-120 DEG C decocts 1.5 hours, and with inorganic ceramic UF membrane, removal of impurities is obtained and decocted for the first time
Liquid;(2) dregs of a decoction of filtering are added into 6 times of water amounts, 80-120 DEG C decocts 1 hour, with hollow fiber ultrafiltration membrane separating and filtering, obtains second
Secondary decoction liquor;(3) merge decoction liquor twice, cooling is 20-30 minutes static at 40 DEG C or less, and 200 mesh net filtrations twice, take
Clear liquid, then be dehydrated with nanofiltration and reverse osmosis membrane, concentration extraction active constituent obtains Chinese medical extract;(4) by the acetic acid of above-mentioned weight
Chlorhexidine is added in ethyl alcohol, is mixed, and 60 DEG C obtain acetic acid chlorhexidine mixed liquor in heating 10 minutes;(5) vinegar is added in cosolvent
It is stirred evenly in sour chlorhexidine mixed liquor, obtains compound bonding solvent;(6) multiple in the mixed solvent stirring is added in Chinese medicinal material extract again
Uniformly, bacteriostatic agent is obtained.
The invention contains the ingredients such as honeysuckle, peppermint, acetic acid chlorhexidine, but it is not mentioned individually for honeysuckle, peppermint
It takes, effective component is not easy to extract;And effective component is acetic acid chlorhexidine in the invention, and contains ethanol content, and both
Ingredient is the ingredient that current professional person attempts to remove in disinfectant, and in addition low deuterium-oxide is not used as base fluid in it, is not had
Wound repair effect.
Work also with sterilizing is used alone in polyhexamethylene guanide (PHMG) 0.2-0.5% recorded in the prior art
With, but polyhexamethylene guanide (PHMG) be used alone the shortcomings that be exactly kill brood cell effect it is weaker, do not reach requirement, inventor
It was found that being compounded with Chinese medicine, synergistic effect can be played, effect is further enhanced.
Chinese Patent Application No. in the prior art: CN201510275419.2, denomination of invention: a kind of disinfection of Chinese drug is spraying
Agent and preparation method thereof, the effective component mass fraction that spray is made include: 11~23 parts of the coptis, 9~21 parts of cordate houttuynia, shoulder
Plate returns 14~28 parts, 10~25 parts of honeysuckle, 6~18 parts of climbing groundsel, 17~29 parts of kuh-seng, 8~26 parts of dandelion, Asiatic plantain 17
~31 parts, 7~22 parts of calamus, 12~27 parts of garlic, 10~24 parts of peach leaf, 13~25 parts of asparagus fern, 6~23 parts of pine needle, frutus cnidii
11~29 parts, 7~26 parts of the tuber of stemona, 12~28 parts of hawthorn, 7~25 parts of menthol, 9~25 parts of the root of Dahurain angelica, 8~25 parts of Radix Angelicae Sinensis, Radix Glycyrrhizae
12~27 parts.It is recorded according to CN201510275419.2 specification, it may have the effect of antibacterial anti-inflammatory, bacterium will not generate anti-
Body, without drug resistance, avoid bacterial dissociation at superbacteria, have no toxic side effect, be using safe external application antibacterial anti-inflammatory medicament and
Preparation method.But this technology is when in use, and medicament need to be applied on gauze, and gauze is wrapped in chest and back or waist and belly, uses
Film is tight in external package, it is seen that this technical solution patient is inconvenient to use, and flavour of a drug are more, in addition higher cost cannot
It is sterilized for circumcision disinfection, the disinfection of neonatal umbilical cord position, skin wound reparation, newborn skin and mucous membrane.
In the prior art: Chinese Patent Application No.: CN201610101457.0, denomination of invention: one kind being used for skin degerming
Thimerosal, the weight of each bulk pharmaceutical chemicals of Chinese medicinal ingredients be respectively coptis 40g~45g, Cortex Phellodendri 25g~30g, cordate houttuynia 15g~
20g, viola mandshurica 10g~15g, Radix Isatidis 20g~25g, wild chrysanthemum 20g~25g, dandelion 10g~15g, semen momordicae 15g~
20g, Golden Larch Bark 10g~15g, honeycomb 15g~20g, garlic 20g~25g, borax 10g~15g, calamine 15g~20g, hedgehog
Skin 5g~10g, cortex dictamni 10g~15g, frutus cnidii 20g~25g, Lappula echinata 5g~10g, fructus ulmi 15g~20g, spina gleditsiae 10g~
15g, catechu 20g~25g, honeysuckle 5g~15g and licorice 15g~20g.According to application No. is CN201010571732.8 to say
Bright secretary carries, this technology has the pathogenic microorganisms such as staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, candida albicans
Stronger killing effect, and it is substantially non-stimulated to skin, have no toxic side effect, performance stablize, not volatile, no bad smell, no
Drug resistance can be generated, but this technology is mainly used for wound skin infection, and flavour of a drug are more, in addition higher cost cannot be used for wrapping
Skin operation sterilizing, the disinfection of neonatal umbilical cord position, newborn skin and mucous membrane disinfection promote skin wound reparation.
In view of the foregoing drawbacks, inventor is by many experiments, research repeatedly, have developed it is a kind of both can be used for sterilizing disappear
Poison, and can be with wound healing, while it can also be safely without secondary land used for pathogen of neonate navel infection, red stern, pustular eruption
New medicine antibiotic preparation.
Summary of the invention
Present invention aim to address fungicide in the prior art effect it is single, Disinfection Effect needs to be further improved,
Safety comprehensive needs the defects of being promoted.For example some disinfectants are only used for wound skin degerming, and flavour of a drug are more, at
Originally higher, irritating to human skin, inconvenient to use etc., and cannot be used for mucous membrane disinfection, newborn skin disinfection, skin wound
It repairs in face.
Another object of the present invention is to provide a kind of skin mucosa disinfectant for wound healing.
It is a further object of the present invention to provide a kind of preparation methods of skin mucosa disinfectant for wound healing.
It is a further object of the present invention to provide one kind to sterilize for skin and mucous membrane shield wound, newborn skin and mucous membrane, is complete
The medical grade sterilizing article of the antibacterials such as whole skin degerming, skin wound reparation.
Skin mucosa disinfectant of the present invention for wound healing is made of the supplementary material of following weight percentage:
Flos Lonicerae extractive solution 1.0%-8.0%, peppermint extracting solution 1.0%-4.5%, Radix Sophorae Flavescentis extractive liquid 1.0%-6.0%, the root of Dahurain angelica extract
Liquid 1.0%-6.0%, polyhexamethylene guanide (PHMG) 0.08%-0.70%, surplus are low deuterium-oxide.
Preferably, the skin mucosa disinfectant of the present invention for wound healing is auxiliary by the original of following weight percentage
Material is made: Flos Lonicerae extractive solution 1.5%-7.5%, peppermint extracting solution 1.2%-4.0%, Radix Sophorae Flavescentis extractive liquid 1.5%-5.5%, white
Root of Dahurian angelica extracting solution 1.5%-5.5%, polyhexamethylene guanide (PHMG) 0.10%-0.65%, surplus are low deuterium-oxide.
It is further preferred that the skin mucosa disinfectant of the present invention for wound healing is by following weight percentage
Supplementary material be made: Flos Lonicerae extractive solution 2.0%-7.0%, peppermint extracting solution 1.6%-3.6%, Radix Sophorae Flavescentis extractive liquid 2.0%-
5.0%, root of Dahurain angelica extracting solution 2.0%-5.0%, polyhexamethylene guanide (PHMG) 0.12%-0.60%, surplus are low deuterium-oxide.
Still more preferably, the skin mucosa disinfectant of the present invention for wound healing is by following weight percentage
The supplementary material of ratio is made: Flos Lonicerae extractive solution 2.5%-6.5%, peppermint extracting solution 1.8%-3.3%, Radix Sophorae Flavescentis extractive liquid 2.5%-
4.5%, root of Dahurain angelica extracting solution 2.5%-4.5%, polyhexamethylene guanide (PHMG) 0.15%-0.55%, surplus are low deuterium-oxide.
Still more preferably, the skin mucosa disinfectant of the present invention for wound healing is by following weight percentage
The supplementary material of ratio is made: Flos Lonicerae extractive solution 3%-5%, peppermint extracting solution 2%-3%, Radix Sophorae Flavescentis extractive liquid 3%-4%, the root of Dahurain angelica mention
Taking liquid 3%-4%, polyhexamethylene guanide (PHMG) 0.2%-0.5%, surplus is low deuterium-oxide.
The skin mucosa disinfectant of wound healing of the present invention, wherein the root of Dahurain angelica extracting solution the preparation method comprises the following steps: claim
Angelica root is taken, impurity is selected, cleans, is ground into coarse powder, adds 5~10 times of 50~90% ethyl alcohol of amount to impregnate, is heated to reflux 2~4
Hour, collect extracting solution;The dregs of a decoction add 3~8 times of 50~90% ethyl alcohol of amount, are heated to reflux 1~3 hour, collect extracting solution, close
And secondary raffinate, filtering obtain root of Dahurain angelica extracting solution.
The skin mucosa disinfectant of wound healing of the present invention, wherein the Flos Lonicerae extractive solution the preparation method comprises the following steps:
Traditional Chinese medicine honeysuckle is weighed, impurity is selected, is cleaned, 8~15 times of amount pure water are added, impregnates 1~2 hour, is heated to 80 DEG C~95
It DEG C extracts 1~3 time, 1~2 hour every time, filtering, filtrate was spare;5~10 times of 60% ethyl alcohol of amount are added in filter residue, are heated to 60 DEG C
~80 DEG C are extracted 1~2 time, and 1~2 hour every time, filtering obtained Flos Lonicerae extractive solution.
The skin mucosa disinfectant of wound healing of the present invention, wherein the peppermint extracting solution the preparation method comprises the following steps: taking
Peppermint medicinal material carries out carbon dioxide supercritical extraction, is 8~10MPa in extracting pressure, extraction temperature is 45~60 DEG C, dioxy
Change carbon gas flow to extract 1~1.5h under conditions of 20~25L/min, collects peppermint oil, be dehydrated with anhydrous sodium sulfate, filtered
Paper filtering, obtains peppermint extracting solution.
The skin mucosa disinfectant of wound healing of the present invention, wherein the Radix Sophorae Flavescentis extractive liquid the preparation method comprises the following steps: taking
Sophora flavescens clean up, and dry, slice, and the water that 8~15 times of weight are added impregnates 2~3h, are heated to 85 DEG C~100 DEG C and mention
It takes 1~2 time, 1~3 hour every time, filters, filtrate is spare;60% ethyl alcohol of 8~10 times of amounts, heating 60 DEG C~80 is added in filter residue
DEG C, it extracts 1~2 time, 1~2h, filtering, merging filtrate obtain Radix Sophorae Flavescentis extractive liquid every time.
The preparation method of the skin mucosa disinfectant of wound healing of the present invention is made of following steps:
1) angelica root is weighed, impurity is selected, cleans, is ground into coarse powder, 5~10 times of 50~90% ethyl alcohol of amount is added to impregnate,
It is heated to reflux 2~4 hours, collects extracting solution;The dregs of a decoction add 3~8 times of 50~90% ethyl alcohol of amount, are heated to reflux 1~3 hour,
Extracting solution is collected, secondary raffinate is merged, filtering obtains root of Dahurain angelica extracting solution;
2) traditional Chinese medicine honeysuckle is weighed, impurity is selected, is cleaned, 8~15 times of amount pure water are added, is impregnated 1~2 hour, heating
It extracts 1~3 time, 1~2 hour every time, filters, filtrate is spare to 80 DEG C~95 DEG C;5~10 times of 60% ethyl alcohol of amount are added in filter residue,
It is heated to 60 DEG C~80 DEG C to extract 1~2 time, 1~2 hour every time, filtering obtained Flos Lonicerae extractive solution;
3) peppermint medicinal material is taken, carbon dioxide supercritical extraction is carried out, is 8~10MPa, extraction temperature 45 in extracting pressure
~60 DEG C, under conditions of carbon dioxide gas flow is 20~25L/min, 1~1.5h is extracted, peppermint oil is collected, with anhydrous sulphur
Sour sodium dehydration, filter paper filter to get peppermint extracting solution;
4) Sophora flavescens are taken, are cleaned up, are dried, slice, the water that 8~15 times of weight are added impregnates 2~3h, is heated to 85
DEG C~100 DEG C extract 1~2 time, 1~3 hour every time, filtering, filtrate was spare;60% ethyl alcohol of 8~10 times of amounts is added in filter residue, adds
60 DEG C~80 DEG C of heat is extracted 1~2 time, and 1~2h, filtering, merging filtrate obtain Radix Sophorae Flavescentis extractive liquid every time;
5) step 1) to the extracting solution in 4) is taken to stir at low speed uniformly mixed, mixing speed is 600~900rpm;When stirring
Between be 5~10min, obtain extraction mixed liquor A;
6) at 35 DEG C~50 DEG C, polyhexamethylene guanide (PHMG) is slowly added in low deuterium-oxide, constant temperature stirring, stirring speed
Degree is 650~900rpm, and mixing time is 5~10min, obtains low deuterium-oxide solution B;
7) the extraction mixed liquor A of step 5) is slowly added to be mixed in the solution B of step 6), mixing speed is
800~1000rpm, time are 10~25min to get skin mucosa disinfectant C.
The present invention can also take following preparation method to be made:
1) each extracting solution is stirred at low speed to uniformly mixed, mixing speed 800rpm;Mixing time is 8min, must be extracted mixed
Close liquid A;
2) at 40 DEG C, polyhexamethylene guanide (PHMG) is slowly added in low deuterium-oxide, constant temperature stirring, mixing speed is
700rpm, mixing time 8min obtain low deuterium-oxide solution B;
3) the extraction mixed liquor A of step 2) is slowly added to be mixed in the solution B of step 3), mixing speed is
900rpm, time are 15min to get skin mucosa disinfectant.
It after thimerosal is made in the present invention, can be used directly, pharmaceutically acceptable auxiliary material can also be added, be made spraying
The regular dosage forms such as agent, ointment, lotion, gel, wet tissue, cold compress patch.
Extracting solution described in inventive formulation uses after being made according to the method for the present invention, can also arrive specialized factory
Qualified extracting solution is bought, is then mixed with the other compositions in formula to get skin mucosa disinfectant of the present invention.
The invention has the following advantages:
1, the present invention is living rich in the plant extractions essence such as honeysuckle, peppermint, kuh-seng, the root of Dahurain angelica and polyhexamethylene guanide (PHMG)
Property ingredient etc., using low deuterium-oxide (DDW) as base fluid, can kill cotton fabric bacterial catalase and fungi, Acanthamoeba cornea
It is scorching pathogen, Escherichia coli, staphylococcus aureus and Candida albicans, skin natural bacteria, inactivation of viruses, bacterial spore, thin
Bacterium brood body etc..And bactericidal effect influenced by environment temperature, pH value and organic matter etc. it is smaller.Thus, it is believed that being completely new
The wide spectrum of a generation, antibacterial agent efficiently, safe and skin mucosa disinfectant.
2, skin mucosa disinfectant of the present invention sterilizes 3min, kills staphylococcus aureus bacterium rate and has reached 99% or more;
And it is 86.87% that comparative example 1, which sterilizes 3min sterilizing rate, it is 87.92% that comparative example 2, which sterilizes 3min sterilizing rate,;Comparative example 3 is sterilized
3min sterilizing rate is 88.69%.The present invention has apparent inhibiting effect to staphylococcus aureus it has been confirmed by experiments that, and is better than
Documents 1,2,3.
3, skin mucosa disinfectant of the present invention sterilizes 3min, kills Escherichia coli rate and has reached 99% or more, and comparative example 1
Sterilizing 3min sterilizing rate is 87.98%, and it is 86.85% that comparative example 2, which sterilizes 3min sterilizing rate,;Comparative example 3 sterilizes 3min sterilizing rate
It is 84.95%.The present invention has apparent inhibiting effect to Escherichia coli it has been confirmed by experiments that, and is better than documents 1,2,3.
4, the present invention can reach 100% to the killing rate of infant skin natural bacteria, and comparative example 1 to infant skin from
Right bacterium killing rate is 89.34%;Comparative example 2 is 88.20% to infant skin natural bacteria killing rate;Comparative example 3 is to infant skin
Natural bacteria killing rate is 90.03%;The present invention has good Disinfection Effect, Disinfection examination to infant skin it has been confirmed by experiments that
Afterwards, baby's skin of abdomen is without ill symptoms such as erythema, detumescences, and effect is better than documents 1,2,3.
5, since the low deuterium-oxide of base fluid of the invention is small-micelle water, it can promote Skin Cell activity, and pass through experiment card
Real present invention wound granulation growth after surgery aspect has significant curative effect, can be shortened the time of wound healing.
6, the present invention can be used for clinical preoperative and postoperative, whole process protection, prevention infection.
7, the present invention can broad-spectrum high efficacy sterilization, be not in bacterial drug resistance;And have long-term bacteriostasis, it can prevent secondary
Pollution.
8, the present invention is to skin and mucosa and eye irritation, mild, without sensitization, and degradable, to environment without dirt
Dye.
9, the present invention is rich in plants antimicrobial essence, and sterilization is more thorough, and protection is more comprehensively.
10, by test, the present invention is free of chlorine without phenol without iodine, without alcohol, without aldehyde, soft non-stimulated, not sensitization, green
Environmental protection, care health.
Specific embodiment
The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention..
Embodiment 1
One, ingredient: Flos Lonicerae extractive solution 1.0%, peppermint extracting solution 1.0%, Radix Sophorae Flavescentis extractive liquid 1.0%, root of Dahurain angelica extracting solution
1.0%, polyhexamethylene guanide (PHMG) 0.08%, surplus are low deuterium-oxide.
Two, this ingredient is chosen any one kind of them implementation by following three kinds of methods.
Preparation method (one):
1) angelica root is weighed, impurity is selected, cleans and is ground into coarse powder, adds 5 times of 50% ethyl alcohol of amount to impregnate, is heated to reflux 2
Hour, collect extracting solution;The dregs of a decoction add 3 times of 50% ethyl alcohol of amount, are heated to reflux 1 hour, collect extracting solution, merge second extraction
Liquid, filtering, obtains root of Dahurain angelica extracting solution;
2) traditional Chinese medicine honeysuckle is weighed, impurity is selected, is cleaned, 8 times of amount pure water are added, is impregnated 1 hour, is heated to 80 DEG C and takes
It 1 time, 1 hour every time, filters, filtrate is spare;5 times of 60% ethyl alcohol of amount are added in filter residue, are heated to 60 DEG C and extract 1 time, 1 is small every time
When, filtering obtains Flos Lonicerae extractive solution;
3) peppermint medicinal material is taken, carbon dioxide supercritical extraction is carried out, is 8MPa in extracting pressure, extraction temperature is 45 DEG C,
Under conditions of carbon dioxide gas flow is 20L/min, 1h is extracted, peppermint oil is collected, is dehydrated with anhydrous sodium sulfate, filter paper mistake
Filter is to get peppermint extracting solution;
4) Sophora flavescens are taken, are cleaned up, are dried, slice, the water that 8 times of weight are added impregnates 2h, is heated to 85 DEG C DEG C and mentions
It takes 1 time, 1 hour every time, filters, filtrate is spare;60% ethyl alcohol of filter residue 8 times of amounts of addition, 60 DEG C of heating, extraction 1 time, every time
1h, filtering, merging filtrate obtain Radix Sophorae Flavescentis extractive liquid;
5) step 1) to the extracting solution in 4) is taken to stir at low speed uniformly mixed, mixing speed 600rpm;Mixing time is
5min obtains extraction mixed liquor A;
6) at 35 DEG C, polyhexamethylene guanide (PHMG) is slowly added in low deuterium-oxide, constant temperature stirring, mixing speed is
650rpm, mixing time 5min obtain low deuterium-oxide solution B;
7) the extraction mixed liquor A of step 5) is slowly added to be mixed in the solution B of step 6), mixing speed is
800rpm, time are 10min to get skin mucosa disinfectant.
Preparation method (two):
1) angelica root is weighed, impurity is selected, cleans and is ground into coarse powder, adds 10 times of 90% ethyl alcohol of amount to impregnate, is heated to reflux 4
Hour, collect extracting solution;The dregs of a decoction add 8 times of 90% ethyl alcohol of amount, are heated to reflux 3 hours, collect extracting solution, merge second extraction
Liquid, filtering, obtains root of Dahurain angelica extracting solution;
2) traditional Chinese medicine honeysuckle is weighed, impurity is selected, is cleaned, 15 times of amount pure water are added, impregnates 2 hours, is heated to 95 DEG C
It extracts 3 times, 2 hours every time, filters, filtrate is spare;10 times of 60% ethyl alcohol of amount are added in filter residue, are heated to 80 DEG C of extractions 2 times, every time
2 hours, filtering obtained Flos Lonicerae extractive solution;
3) peppermint medicinal material is taken, carbon dioxide supercritical extraction is carried out, is 10MPa in extracting pressure, extraction temperature is 60 DEG C,
Under conditions of carbon dioxide gas flow is 25L/min, 1.5h is extracted, peppermint oil is collected, is dehydrated with anhydrous sodium sulfate, filter paper mistake
Filter is to get peppermint extracting solution;
4) Sophora flavescens are taken, are cleaned up, are dried, slice, the water that 15 times of weight are added impregnates 3h, is heated to 100 DEG C and mentions
It takes 2 times, 3 hours every time, filters, filtrate is spare;60% ethyl alcohol of filter residue 10 times of amounts of addition, 80 DEG C of heating, extraction 2 times, every time
2h, filtering, merging filtrate obtain Radix Sophorae Flavescentis extractive liquid;
5) step 1) to the extracting solution in 4) is taken to stir at low speed uniformly mixed, mixing speed 900rpm;Mixing time is
10min obtains extraction mixed liquor A;
6) at 50 DEG C, polyhexamethylene guanide (PHMG) is slowly added in low deuterium-oxide, constant temperature stirring, mixing speed is
900rpm, mixing time 10min obtain low deuterium-oxide solution B;
7) the extraction mixed liquor A of step 5) is slowly added to be mixed in the solution B of step 6), mixing speed is
1000rpm, time are 25min to get skin mucosa disinfectant.
Preparation method (three):
1) angelica root is weighed, impurity is selected, cleans and is ground into coarse powder, adds 8 times of 75% ethyl alcohol of amount to impregnate, is heated to reflux 3
Hour, collect extracting solution;The dregs of a decoction add 5 times of 75% ethyl alcohol of amount, are heated to reflux 2 hours, collect extracting solution, merge second extraction
Liquid, filtering, obtains root of Dahurain angelica extracting solution;
2) traditional Chinese medicine honeysuckle is weighed, impurity is selected, is cleaned, 12 times of amount pure water are added, impregnates 1.5 hours, is heated to 85
It DEG C extracts 2 times, 1.5 hours every time, filtering, filtrate was spare;8 times of 60% ethyl alcohol of amount are added in filter residue, are heated to 70 DEG C and extract 1.5
Secondary, 1.5 hours every time, filtering obtained Flos Lonicerae extractive solution;
3) peppermint medicinal material is taken, carbon dioxide supercritical extraction is carried out, is 9MPa in extracting pressure, extraction temperature is 50 DEG C,
Under conditions of carbon dioxide gas flow is 22L/min, 1h is extracted, peppermint oil is collected, is dehydrated with anhydrous sodium sulfate, filter paper mistake
Filter is to get peppermint extracting solution;
4) Sophora flavescens are taken, are cleaned up, are dried, slice, the water that 12 times of weight are added impregnates 2h, is heated to 90 DEG C of extractions
It 2 times, 1.5 hours every time, filters, filtrate is spare;60% ethyl alcohol of filter residue 9 times of amounts of addition, 70 DEG C of heating, extraction 2 times, every time
1.5h, filtering, merging filtrate obtain Radix Sophorae Flavescentis extractive liquid;
5) step 1) to the extracting solution in 4) is taken to stir at low speed uniformly mixed, mixing speed 800rpm;Mixing time is
8min obtains extraction mixed liquor A;
6) at 40 DEG C, polyhexamethylene guanide (PHMG) is slowly added in low deuterium-oxide, constant temperature stirring, mixing speed is
700rpm, mixing time 8min obtain low deuterium-oxide solution B;
7) the extraction mixed liquor A of step 5) is slowly added to be mixed in the solution B of step 6), mixing speed is
900rpm, time are 15min to get skin mucosa disinfectant.
Preparation method (four)-specialized market purchase extracting solution
1) each extracting solution is stirred at low speed to uniformly mixed, mixing speed 800rpm;Mixing time is 8min, must be extracted mixed
Close liquid A;
2) at 40 DEG C, polyhexamethylene guanide (PHMG) is slowly added in low deuterium-oxide, constant temperature stirring, mixing speed is
700rpm, mixing time 8min obtain low deuterium-oxide solution B;
3) the extraction mixed liquor A of step 1) is slowly added to be mixed in the solution B of step 2), mixing speed is
900rpm, time are 15min to get skin mucosa disinfectant.
Embodiment 2
Ingredient: Flos Lonicerae extractive solution 8.0%, peppermint extracting solution 4.5%, Radix Sophorae Flavescentis extractive liquid 6.0%, root of Dahurain angelica extracting solution
6.0%%, polyhexamethylene guanide (PHMG) 0.70%, surplus are low deuterium-oxide.
The preparation method is the same as that of Example 1.
Embodiment 3
Ingredient: Flos Lonicerae extractive solution 1.5%, peppermint extracting solution 1.2%, Radix Sophorae Flavescentis extractive liquid 1.5%, root of Dahurain angelica extracting solution
1.5%, polyhexamethylene guanide (PHMG) 0.10%, surplus are low deuterium-oxide.
The preparation method is the same as that of Example 1.
Embodiment 4
Ingredient: Flos Lonicerae extractive solution 7.5%, peppermint extracting solution 4.0%, Radix Sophorae Flavescentis extractive liquid 5.5%, root of Dahurain angelica extracting solution
5.5%, polyhexamethylene guanide (PHMG) 0.65%, surplus are low deuterium-oxide.
The preparation method is the same as that of Example 1.
Embodiment 5
Ingredient: Flos Lonicerae extractive solution 2.0%, peppermint extracting solution 1.6%, Radix Sophorae Flavescentis extractive liquid 2.0%, root of Dahurain angelica extracting solution
2.0%, polyhexamethylene guanide (PHMG) 0.12%, surplus are low deuterium-oxide.
The preparation method is the same as that of Example 1.
Embodiment 6
Ingredient: Flos Lonicerae extractive solution 7.0%, peppermint extracting solution 3.6%, Radix Sophorae Flavescentis extractive liquid 5.0%, root of Dahurain angelica extracting solution
5.0%, polyhexamethylene guanide (PHMG) 0.60%, surplus are low deuterium-oxide.
The preparation method is the same as that of Example 1.
Embodiment 7
Ingredient: Flos Lonicerae extractive solution 2.5%, peppermint extracting solution 1.8%, Radix Sophorae Flavescentis extractive liquid 2.5%, root of Dahurain angelica extracting solution
2.5%, polyhexamethylene guanide (PHMG) 0.15%, surplus are low deuterium-oxide.
The preparation method is the same as that of Example 1.
Embodiment 8
Ingredient: Flos Lonicerae extractive solution 6.5%, peppermint extracting solution 3.3%, Radix Sophorae Flavescentis extractive liquid 4.5%, root of Dahurain angelica extracting solution
4.5%, polyhexamethylene guanide (PHMG) 0.55%, surplus are low deuterium-oxide.
The preparation method is the same as that of Example 1.
Embodiment 9
Ingredient: Flos Lonicerae extractive solution 3%, peppermint extracting solution 2%, Radix Sophorae Flavescentis extractive liquid 3%, root of Dahurain angelica extracting solution 3%, poly- six Asia
Methylguanidine (PHMG) 0.2%, surplus are low deuterium-oxide.
The preparation method is the same as that of Example 1.
Embodiment 10
Ingredient: Flos Lonicerae extractive solution 5%, peppermint extracting solution 3%, Radix Sophorae Flavescentis extractive liquid 4%, root of Dahurain angelica extracting solution 4%, poly- six Asia
Methylguanidine (PHMG) 0.5%, surplus are low deuterium-oxide.
The preparation method is the same as that of Example 1.
Embodiment 11
Ingredient: Flos Lonicerae extractive solution 3%, peppermint extracting solution 2%, Radix Sophorae Flavescentis extractive liquid 3%, root of Dahurain angelica extracting solution 3%, poly- six Asia
Methylguanidine (PHMG) 0.2%, surplus are low deuterium-oxide.
Preparation method:
1) each extracting solution is stirred at low speed to uniformly mixed, mixing speed 800rpm;Mixing time is 8min, must be extracted mixed
Close liquid A;
2) at 40 DEG C, polyhexamethylene guanide (PHMG) is slowly added in low deuterium-oxide, constant temperature stirring, mixing speed is
700rpm, mixing time 8min obtain low deuterium-oxide solution B;
3) the extraction mixed liquor A of step 1) is slowly added to be mixed in the solution B of step 2), mixing speed is
900rpm, time are 15min to get skin mucosa disinfectant.
Embodiment 12
Ingredient: Flos Lonicerae extractive solution 5%, peppermint extracting solution 3%, Radix Sophorae Flavescentis extractive liquid 4%, root of Dahurain angelica extracting solution 4%, poly- six Asia
Methylguanidine (PHMG) 0.5%, surplus are low deuterium-oxide.
The preparation method is the same as that of Example 11.
For the ingredient and effect that the present invention is further explained, inventor carries out the ingredient of raw material, extraction, moulding process
A large amount of experimental study is taken passages as follows:
One, ingredient origin is discussed:
By experimental study, the Flos Lonicerae extractive solution in the present invention has clearing heat and detoxicating, promotes incarnant effect, because
This this ingredient plays antipyretic and antidote functions in the present invention, through inventor's repetition test, discovery Flos Lonicerae extractive solution compatibility
Peppermint extracting solution, bactericidal effect further increase, and can solve the symptom of patient skin itch, moreover it is possible to shrink capilary;In order to
Further develop the good ingredient of effect, inventor with honeysuckle, two taste medicine of peppermint come when carrying out disinfection, effect is not obvious,
Therefore it finds not only be directed to after Radix Sophorae Flavescentis extractive liquid, polyhexamethylene guanide (PHMG) are added in component by repetition test
Whole skin degerming can also be sterilized for damaged skin, and antibiotic and sterilizing effect is ideal, and safety is very high, does not almost have
There is side effect;But apparent effect is not had for skin wound reparation, then inventor has carried out a large amount of screening examination again
It tests, in the ingredient before root of Dahurain angelica extracting solution is added to, while replacing conventional purification water as base fluid using low deuterium-oxide, because the root of Dahurain angelica mentions
Take liquid play the role of it is good remove the rotten and let fresh grow, apocenosis myogenic along with low deuterium-oxide is by isotope separation technique removes purified water
In the D-atom that is harmful to the human body, and low deuterium-oxide is small-micelle water through nuclear magnetic resonance measuring, is very beneficial for promoting skin
Local metabolic.It can promote Wound fibroblasts and synthesize each extracellular matrix secretion and various growth factors, accelerate surface of a wound blood vessel
Change process is conducive to surface of a wound edge skin progenitor cell and is proliferated and migrates, breaks up, wound healing.Entire formula both can be used for
All kinds of bacterium sterilizings, and skin and mucous membrane wound healing can be promoted, at the same can also safety it is without side-effects for new life
Youngster's infection of umbilicus, the medical treatment and nursing of red stern, pustular eruption.
Two, effective component and killing microbe sort:
The present invention is rich in the plant extractions essence such as honeysuckle, peppermint, kuh-seng, the root of Dahurain angelica and polyhexamethylene guanide (PHMG) activity
Ingredient etc. can kill cotton fabric bacterial catalase and fungi, Acanthamoeba Keratitis using low deuterium-oxide (DDW) as base fluid
Pathogen, Escherichia coli, staphylococcus aureus and Candida albicans, skin natural bacteria, inactivation of viruses, bacterial spore, bacterium
Brood body etc..And bactericidal effect influenced by environment temperature, pH value and organic matter etc. it is smaller, thus, it is believed that being completely new one
The skin mucosa disinfectant of the wide spectrum in generation, antibacterial agent efficiently, safe and wound healing.
Three, sterilization mechanism
The present invention is the herbal mixture antibiotic preparation of Chinese medical extract Yu polyhexamethylene guanide (PHMG), in present component
Polyhexamethylene guanide (PHMG) have very high activity, easily compound, be dissolved in after water into electropositive, cation and micro- life in molecule
The anion Electrostatic Absorption of object cell surface, the bacteriolyze enzyme effect of block cell, makes cell surface structural degeneration or destruction, thus
Inhibit bacterial reproduction;Again because it is high polymer, molecule has polydispersity, has been formed by adsorption capacity not to microorganism
Together, thus its to microorganism have nonspecific killing mode;Nonspecific physics mode sterilization, bacterium are nearly free from drug resistance.
Polyhexamethylene guanide (PHMG) in present component is cation superpolymer, is distributed as 16 or 16 or more
Biguanide units, each biguanide units have 2 cation groups, the polyhexamethylene guanide (PHMG) and skin surface energy of high molecular weight
Multiple absorption point is formed, bond strength is higher.Part of cation group is in conjunction with skin surface, and free cationic base
Good antibacterial action still plays in group, so can long acting antibiotic.
Chinese medical extract in the present invention is honeysuckle, peppermint, kuh-seng, the root of Dahurain angelica respectively, modern study proves that, honeysuckle contains
Have the pharmacological components such as chlorogenic acid, cyanidenon glycosides, to the various pathogens such as hemolytic streptococcus, Staphylococcus aureus have compared with
Strong restraint, peppermint have the function of sweating and heat resolving, sterilizing, change it is turbid ward off it is dirty.Kuh-seng has the function of antibacterial anti-inflammatory, right
Propiobacterium, staphylococcus aureus, Escherichia coli have inhibiting effect, can antiallergy, analgesia.
Four, the research of 1 raw material proportioning of test example
1, the present invention is with Flos Lonicerae extractive solution, peppermint extracting solution, Radix Sophorae Flavescentis extractive liquid, root of Dahurain angelica extracting solution, polyhexamethylene
Guanidine (PHMG), a kind of skin mucosa disinfectant for wound healing that surplus is the preparation of low deuterium-oxide, name of product are as follows: contain source element
The characteristics of wound repair liquid, above-mentioned each taste ingredient is due to itself, different effects is played, to make product embody its effect, it is necessary to
The ratio of various composition is reasonably adjusted, while guaranteeing to have no toxic side effect, so company inventor is first to the above raw material honeysuckle
Extracting solution, peppermint extracting solution proportion studied, screened using orthogonal experiment, factor level please refers to 1 institute of table
Show.
1 orthogonal test factor level of table
Orthogonal arrage makees orthogonal test conceptual design, obtains by carrying out range analysis to orthogonal test:
Feasible proportion are as follows:
1) Flos Lonicerae extractive solution 1.0-8.0%, peppermint extracting solution 1.0-4.5%.
2) Flos Lonicerae extractive solution 1.5-7.5%, peppermint extracting solution 1.2-4.0%.
Preferably proportion are as follows:
1) Flos Lonicerae extractive solution 2.0-7.0%, peppermint extracting solution 1.6-3.6%.
2) Flos Lonicerae extractive solution 2.5-6.5%, peppermint extracting solution 1.8-3.3%.
Optimum proportioning is (A1):
Flos Lonicerae extractive solution 3%-5%, peppermint extracting solution 2%-3%.
In order to further develop the good ingredient of effect, inventor when sterilizing, is sent out with honeysuckle, two taste medicine of peppermint
Existing effect is not obvious, therefore finds that kuh-seng, polyhexamethylene guanide (PHMG) are added in component rear antibacterial by repetition test
Disinfection is ideal, and safety is very high, almost without side effect;The optimum proportioning that above-mentioned orthogonal test is obtained
Code name is set to a1 (Flos Lonicerae extractive solution 3%-5%, peppermint extracting solution 2%-3%) to carry out orthogonal test, in test, a1 because
Element is constant, only carries out the selection of Radix Sophorae Flavescentis extractive liquid, polyhexamethylene guanide (PHMG), to obtain suitable proportion, uses orthogonal examination
The method of testing is screened, and factor level please refers to shown in table 2.
2 orthogonal test factor level of table
Orthogonal arrage makees orthogonal test conceptual design, obtains by carrying out range analysis to orthogonal test:
Feasible proportion are as follows:
1, a1, Radix Sophorae Flavescentis extractive liquid 1.0%-6.0%, polyhexamethylene guanide (PHMG) 0.08%-0.70%.
2, a1, Radix Sophorae Flavescentis extractive liquid 1.5%-5.5%, polyhexamethylene guanide (PHMG) 0.10%-0.65%.
Preferably proportion are as follows:
1, a1, Radix Sophorae Flavescentis extractive liquid 2.0%-5.0%, polyhexamethylene guanide (PHMG) 0.12%-0.60%.
2, a1, Radix Sophorae Flavescentis extractive liquid 2.5%-4.5%, polyhexamethylene guanide (PHMG) 0.15%-0.55%.
Optimum proportioning is (a2):
A1, Radix Sophorae Flavescentis extractive liquid 3%-4%, polyhexamethylene guanide (PHMG) 0.2%-0.5%.
Inventor is apparent in order to play the role of this product to skin wound reparation, has then carried out a large amount of screening examination
It tests, a2 (a1, Radix Sophorae Flavescentis extractive liquid 3%-4%, polyhexamethylene guanide is set to the optimum proportioning code name that above-mentioned orthogonal test obtains
(PHMG) 0.2%-0.5%) orthogonal test is carried out, in test, a2 factor is constant, before root of Dahurain angelica extracting solution is added to
It in ingredient, while replacing conventional purification water as basigamy using low deuterium-oxide, specifically carries out root of Dahurain angelica extracting solution using orthogonal experiment
Screening, low deuterium-oxide are not done experiment research, and factor level please refers to shown in table 3.
3 orthogonal test factor level of table
Orthogonal arrage makees orthogonal test conceptual design, obtains by carrying out range analysis to orthogonal test:
Feasible proportion are as follows:
1) a2, root of Dahurain angelica extracting solution 1.0%-6.0%.
2) a2, root of Dahurain angelica extracting solution 1.5%-5.5%.
Preferably proportion are as follows:
1) a2, root of Dahurain angelica extracting solution 2.0%-5.0%.
2) a2, root of Dahurain angelica extracting solution 2.5%-4.5%.
Optimum proportioning are as follows:
A2, root of Dahurain angelica extracting solution 3%-4%.
It is obtained by 3 orthogonal tests:
The feasible technical solution proportion of the present invention is as follows:
1) Flos Lonicerae extractive solution 1.0%-8.0%, peppermint extracting solution 1.0%-4.5%, Radix Sophorae Flavescentis extractive liquid 1.0%-
6.0%, root of Dahurain angelica extracting solution 1.0%-6.0%, polyhexamethylene guanide (PHMG) 0.08%-0.70%, surplus are low deuterium-oxide.
2) Flos Lonicerae extractive solution 1.5%-7.5%, peppermint extracting solution 1.2%-4.0%, Radix Sophorae Flavescentis extractive liquid 1.5%-
5.5%, root of Dahurain angelica extracting solution 1.5%-5.5%, polyhexamethylene guanide (PHMG) 0.10%-0.65%, surplus are low deuterium-oxide.
Preferably proportion are as follows:
1) Flos Lonicerae extractive solution 2.0%-7.0%, peppermint extracting solution 1.6%-3.6%, Radix Sophorae Flavescentis extractive liquid 2.0%-
5.0%, root of Dahurain angelica extracting solution 2.0%-5.0%, polyhexamethylene guanide (PHMG) 0.12%-0.60%, surplus are low deuterium-oxide.
2) Flos Lonicerae extractive solution 2.5%-6.5%, peppermint extracting solution 1.8%-3.3%, Radix Sophorae Flavescentis extractive liquid 2.5%-
4.5%, root of Dahurain angelica extracting solution 2.5%-4.5%, polyhexamethylene guanide (PHMG) 0.15%-0.55%, surplus are low deuterium-oxide.
Optimum proportioning are as follows:
Flos Lonicerae extractive solution 3%-5%, peppermint extracting solution 2%-3%, Radix Sophorae Flavescentis extractive liquid 3%-4%, root of Dahurain angelica extracting solution
3%-4%, polyhexamethylene guanide (PHMG) 0.2%-0.5%, surplus are low deuterium-oxide.
Five, inventor inventor provides following tests data in order to further illustrate beneficial effects of the present invention:
Experimental example
One, material and method
1, laboratory sample: the product of the embodiment of the present invention 9, the product of embodiment 10, the product of embodiment 11, comparative example 1
(number of patent application: CN201310409073.1), comparative example 2 (polyhexamethylene guanide (PHMG)), comparative example 3 (number of patent application:
CN201610101457.0)。
2, test article concentration:
1) stoste of the embodiment of the present invention 9;
2) stoste of the embodiment of the present invention 10;
3) stoste of the embodiment of the present invention 11;
4) comparative example 1 (sharp application number: CN201310409073.1), stoste;
5) comparative example 2 (polyhexamethylene guanide (PHMG)), 0.3% solution;
6) comparative example 3 (CN201610101457.0), stoste.
3, experimental bacteria: reference culture (staphylococcus aureus, Escherichia coli) provided by Guizhou Province's medicine inspection is come from.
4, method: selecting medical staff 40 in hospital ward, ewborn infant 40 of 2-25 days of birth, the reference Ministry of Public Health
" disinfection technology standard " carries out skin degerming.
5, sample preparation methods: the embodiment of the present invention 9 is produced with embodiment 10 according to the preparation method (one) in embodiment 1
Product;The product that embodiment 11 is produced according to 11 preparation methods are implemented.
6,1 component of comparative example and preparation method: Chinese Patent Application No.: CN201310409073.1, denomination of invention: a kind of
The bacteriostatic agent of sterilizing and skin mucosa disinfectant and preparation method thereof, it is made of the raw material in following weight range:
Honeysuckle 15-25kg, bo-chrysanthemum 15-25kg, peppermint 15-25kg, dandelion 15-25kg, folium isatidis 3-8kg, climbing groundsel 3-
8kg, tribulus terrestris 3-8kg, acetic acid chlorhexidine, 0.9-0.12kg, cosolvent 0.1-0.3kg, ethyl alcohol 0.1-0.3kg.Preparation method:
(1) honeysuckle of the weight, bo-chrysanthemum, peppermint, dandelion, folium isatidis, climbing groundsel, tribulus terrestris are put into molten device of boiling medicine to decoct
Secondary, for the first time plus 8 times of water are measured, and are impregnated 30 minutes, and 80-120 DEG C decocts 1.5 hours, and with inorganic ceramic UF membrane, removal of impurities is obtained
First time decoction liquor;(2) dregs of a decoction of filtering are added into 6 times of water amounts, 80-120 DEG C decocts 1 hour, is separated with hollow fiber ultrafiltration membrane
Filtering, obtains second of decoction liquor;(3) merge decoction liquor twice, cooling, 200 mesh screen mistakes 20-30 minutes static at 40 DEG C or less
Filter twice, takes supernatant, then be dehydrated with nanofiltration and reverse osmosis membrane, and concentration extraction active constituent obtains Chinese medical extract;It (4) will be upper
The acetic acid chlorhexidine for stating weight is added in ethyl alcohol, is mixed, and 60 DEG C obtain acetic acid chlorhexidine mixed liquor in heating 10 minutes;(5) will
Cosolvent is added in acetic acid chlorhexidine mixed liquor and stirs evenly, and obtains compound bonding solvent;(6) compound is added in Chinese medicinal material extract again
It is stirred evenly in bonding solvent, obtains bacteriostatic agent.
7, comparative example 2: polyhexamethylene guanide (PHMG) is bought in Shanghai Gaoju Biotechnology Co., Ltd..
8,3 component of comparative example and preparation method: Chinese Patent Application No.: CN201610101457.0, denomination of invention: a kind of
For the thimerosal of skin degerming, the weight of each bulk pharmaceutical chemicals of Chinese medicinal ingredients is respectively coptis 40g~45g, Cortex Phellodendri 25g~30g, fish
Raw meat grass 15g~20g, viola mandshurica 10g~15g, Radix Isatidis 20g~25g, wild chrysanthemum 20g~25g, dandelion 10g~15g, wood
Soft-shelled turtle 15g~20g, Golden Larch Bark 10g~15g, honeycomb 15g~20g, garlic 20g~25g, borax 10g~15g, calamine 15g
~20g, hedgehog skin 5g~10g, cortex dictamni 10g~15g, frutus cnidii 20g~25g, Lappula echinata 5g~10g, fructus ulmi 15g~20g, soap
10g~15g, catechu 20g~25g, honeysuckle 5g~15g and licorice 15g~20g are pierced in angle.Preparation method: the first step, preparation
The traditional Chinese medicine ingredients;Second step weighs deionized water by weight, after the deionized water of half is heated to 60~70 DEG C, is added
In reaction kettle, the nonylphenol polyoxyethylene ether and lauryl sodium sulfate that above-mentioned weight is then added are stirred evenly, etc. temperature drop
When to 30~40 DEG C, be added above-mentioned weight traditional Chinese medicine ingredients and remaining deionized water and absolute ethyl alcohol and stirring it is uniform, filtering obtains
To thimerosal.
Two, result
1, neutralizer qualification test rank and result:
1) neutralizer qualification test group: the 1st group: thimerosal+bacteria suspension;2nd group of (thimerosal+bacteria suspension)+neutralizer;
3rd group of neutralizer+bacteria suspension;4th group of (thimerosal+neutralizer)+bacteria suspension;5th group of dilution+bacteria suspension;6th group of dilution
Neutralizer culture medium.
2) neutralizer authentication test results are with the lecithin of the sodium thiosulfate+0.28% of 3% Tween-80+4g/L
Phosphate buffer solution makees neutralizer, can effectively neutralize effect of the phage surface residual disinfectancy liquid to staphylococcus aureus, and
And the neutralizer and its neutralized reaction product have no adverse effects to test organisms growth, are specifically shown in Table 4:
Table 4;Neutralizer authentication test results table
2, to the bactericidal effect of staphylococcus aureus
Under normal temperature conditions, being repeated test three times proves that the present invention and comparative example the results are shown in Table 5:
Table 5: bactericidal effect table of the different time to staphylococcus aureus
The results show that the preparation sterilization 3min of the embodiment of the present invention 9 reaches staphylococcus aureus sterilizing rate
99.99%, the preparation sterilization 3min sterilizing rate of embodiment 10 has reached 99.97%, the preparation sterilization 3min sterilization of embodiment 11
Rate has reached 99.85%;And it is 86.87% that comparative example 1, which sterilizes 3min sterilizing rate, comparative example 2 sterilizes 3min sterilizing rate and is
87.92%;It is 88.69% that comparative example 3, which sterilizes 3min sterilizing rate,.As it can be seen that 9,10,11 pairs of Staphylococcus aureus of the embodiment of the present invention
Bacterium has apparent inhibiting effect, and is better than documents 1,2,3.
3, to the bactericidal effect of Escherichia coli
Under normal temperature conditions, being repeated test three times proves that the present invention and comparative example the results are shown in Table 6:
Table 6: bactericidal effect table of the different time to Escherichia coli
The results show that the preparation sterilization 3min Escherichia coli sterilizing rate of the embodiment of the present invention 9 has reached 99.95%, has implemented
The preparation sterilization 3min sterilizing rate of example 10 has reached 99.95%, and the preparation sterilization 3min sterilizing rate of embodiment 11 reaches
99.96%, and it is 87.98% that comparative example 1, which sterilizes 3min sterilizing rate, it is 86.85% that comparative example 2, which sterilizes 3min sterilizing rate,;Comparison
It is 84.95% that example 3, which sterilizes 3min sterilizing rate,.As it can be seen that 9,10 pairs of Escherichia coli of the embodiment of the present invention have apparent inhibiting effect, and
Better than documents 1,2,3.
4, to hand-wrist bones field test results
Under normal temperature conditions, being repeated test three times proves, the results are shown in Table 7:
Table 7: to hand-wrist bones field test results table
By upper table it is found that the killing rate of 9 hand-wrist bones field test of the embodiment of the present invention is 97.97%, embodiment 10
Killing rate is 96.96%, and the killing rate of embodiment 11 is 96.59%, and the killing rate of documents 1 is 88.95%;Documents
2 killing rate is 86.99%;The killing rate of documents 3 is 92.44%;As it can be seen that the present invention has good Disinfection Effect,
And it is better than documents 1,2,3.
5, infant skin sterilizes site test results
By the embodiment of the present invention 9, embodiment 10, the stoste of embodiment 11, documents 1,2,3 product respectively to baby
Skin of abdomen is embrocated, disinfection 1min, is shown in Table 8 to the bactericidal effect of skin surface natural bacteria:
8 infant skin of table sterilizes field test table
By upper table it is found that the embodiment of the present invention 9 is 100% to the killing rate of infant skin natural bacteria, 10 baby of embodiment
Youngster's skin natural bacteria killing rate is 99.51%, and 11 infant skin natural bacteria killing rate of embodiment is 99.14%;Documents 1 are right
Infant skin natural bacteria killing rate is 89.34%;Documents 2 are 88.20% to infant skin natural bacteria killing rate;Comparison text
Part 3 is 90.03% to infant skin natural bacteria killing rate;As it can be seen that the present invention has good Disinfection Effect to infant skin, disappear
After poison detection, baby's skin of abdomen is without ill symptoms such as erythema, detumescences, and effect is better than documents 1,2,3.
6, surface of a wound recovery from illness test
1) general material: choosing Kunming small white mouse 60, is divided into 6 groups of (embodiment of the present invention 9, the embodiment of the present invention 10, sheets
The product of inventive embodiments 11, documents 1, documents 2, documents 3).
2) test method:
The wound that mouse is drawn to long 2cm, depth 0.5cm coats the thimerosal of the present invention and documents respectively, compares the surface of a wound
Healing time the results are shown in Table 9:
Table 9: wound healing time compares
As known from Table 9, the time needed for 9,10,11 wound healing of the embodiment of the present invention is significantly shorter than documents 1,2,3
The required time.Illustrate that present invention wound granulation growth after surgery aspect has significant curative effect.
7, other Test Summaries
(1) sample: since process flow is the same, the only difference of data, thus only take 9 sample of the embodiment of the present invention into
Row correlation test, see Table 10 for details for test result.
1) sample preparation methods: the product that the embodiment of the present invention 9 is produced according to the preparation method (one) in embodiment 1.
Table 10: correlation test result table
8, this product and hospital often with sterile products compared with, the results are shown in Table 11:
Conclusion: this product is free of chlorine without phenol without iodine, without alcohol, without aldehyde, soft non-stimulated, not sensitization.It is environmentally protective, care
Health.Due to the low deuterium-oxide of the base fluid of this product be small-micelle water, can promote Skin Cell activity, so can be shortened wound healing when
Between.
It summarizes, the present invention is rich in plant extractions essence and polyhexamethylene guanides such as honeysuckle, peppermint, kuh-seng, the roots of Dahurain angelica
(PHMG) active constituent etc., using low deuterium-oxide (DDW) as base fluid, can kill cotton fabric bacterial catalase and fungi, spine Ah
Rice bar keratitis pathogen, Escherichia coli, staphylococcus aureus and Candida albicans, skin natural bacteria, inactivation of viruses, bacterium
Brood cell, bacterial propagule etc..And bactericidal effect influenced by environment temperature, pH value and organic matter etc. it is smaller.Thus, it is believed that
It is the wide spectrum, antibacterial agent efficiently, safe and skin mucosa disinfectant of all new generation.Skin mucosa disinfectant sterilization of the present invention
3min kills staphylococcus aureus bacterium rate and has reached 99% or more;And comparative example 1 sterilizes 3min and kills staphylococcus aureus
Bacterium rate is 86.87%, and it is 87.92% that comparative example 2, which sterilizes 3min and kills staphylococcus aureus bacterium rate,;Comparative example 3 sterilizes 3min
Killing staphylococcus aureus bacterium rate is 88.69%.The present invention has apparent inhibition to staphylococcus aureus it has been confirmed by experiments that
Effect, and it is better than documents 1,2,3.Skin mucosa disinfectant of the present invention sterilizes 3min, kills Escherichia coli rate and reaches
99% or more, and it is 87.98% that comparative example 1, which sterilizes 3min to kill Escherichia coli rate, comparative example 2 sterilizes 3min and kills Escherichia coli
Rate is 86.85%;It is 84.95% that comparative example 3, which sterilizes 3min and kills Escherichia coli rate,.The present invention is to large intestine bar it has been confirmed by experiments that
Bacterium has apparent inhibiting effect, and is better than documents 1,2,3.The present invention reaches as high as the killing rate of infant skin natural bacteria
To 100%, and comparative example 1 is 89.34% to infant skin natural bacteria killing rate;Comparative example 2 kills infant skin natural bacteria
Rate is 88.20%;Comparative example 3 is 90.03% to infant skin natural bacteria killing rate;The present invention is to baby's skin it has been confirmed by experiments that
Skin has good Disinfection Effect, and after Disinfection examination, baby's skin of abdomen is without ill symptoms such as erythema, detumescences, and effect is better than
Documents 1,2,3.Since the low deuterium-oxide of base fluid of the invention is small-micelle water, Skin Cell activity can promote, and pass through reality
Verifying real present invention wound granulation growth after surgery aspect has significant curative effect, can be shortened the time of wound healing.The present invention is available
In clinical preoperative and postoperative, whole process protection, prevention infection.The present invention can broad-spectrum high efficacy sterilization, be not in bacterial drug resistance;And have
Long-term bacteriostasis can prevent secondary pollution.The present invention is mild, without sensitization to skin and mucosa and eye irritation, and can
Degradation, there are no pollution to the environment.The present invention is rich in plants antimicrobial essence, and sterilization is more thorough, and protection is more comprehensively.By test, originally
Invention is free of chlorine without phenol without iodine, without alcohol, without aldehyde, and soft non-stimulated, sensitization, not environmentally protective, care health.
Although above having used general explanation, specific embodiment and test, the present invention is made to retouch in detail
State, but on the basis of the present invention, it can be made it is some modify or improve, this is aobvious and easy to those skilled in the art
See.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, belong to claimed
Range.
Claims (10)
1. a kind of skin mucosa disinfectant for wound healing, which is characterized in that by the supplementary material system of following weight percentage
At: Flos Lonicerae extractive solution 1.0%-8.0%, peppermint extracting solution 1.0%-4.5%, Radix Sophorae Flavescentis extractive liquid 1.0%-6.0%, the root of Dahurain angelica mention
Taking liquid 1.0%-6.0%, polyhexamethylene guanide 0.08%-0.70%, surplus is low deuterium-oxide.
2. the skin mucosa disinfectant of wound healing according to claim 1, which is characterized in that by following weight percentage
Supplementary material is made: Flos Lonicerae extractive solution 1.5%-7.5%, peppermint extracting solution 1.2%-4.0%, Radix Sophorae Flavescentis extractive liquid 1.5%-
5.5%, root of Dahurain angelica extracting solution 1.5%-5.5%, polyhexamethylene guanide 0.10%-0.65%, surplus are low deuterium-oxide.
3. the skin mucosa disinfectant of wound healing according to claim 2, which is characterized in that by following weight percentage
Supplementary material is made: Flos Lonicerae extractive solution 2.0%-7.0%, peppermint extracting solution 1.6%-3.6%, Radix Sophorae Flavescentis extractive liquid 2.0%-
5.0%, root of Dahurain angelica extracting solution 2.0%-5.0%, polyhexamethylene guanide 0.12%-0.60%, surplus are low deuterium-oxide.
4. the skin mucosa disinfectant of wound healing according to claim 3, which is characterized in that by following weight percentage
Supplementary material is made: Flos Lonicerae extractive solution 2.5%-6.5%, peppermint extracting solution 1.8%-3.3%, Radix Sophorae Flavescentis extractive liquid 2.5%-
4.5%, root of Dahurain angelica extracting solution 2.5%-4.5%, polyhexamethylene guanide 0.15%-0.55%, surplus are low deuterium-oxide.
5. the skin mucosa disinfectant of wound healing according to claim 4, which is characterized in that by following weight percentage
Supplementary material is made: Flos Lonicerae extractive solution 3%-5%, peppermint extracting solution 2%-3%, Radix Sophorae Flavescentis extractive liquid 3%-4%, root of Dahurain angelica extracting solution
3%-4%, polyhexamethylene guanide 0.2%-0.5%, surplus are low deuterium-oxide.
6. the skin mucosa disinfectant of any one of -5 wound healings according to claim 1, which is characterized in that the root of Dahurain angelica mentions
Take liquid the preparation method comprises the following steps: weighs angelica root, selects impurity, cleans, be ground into coarse powder, add 5~10 times of 50~90% second of amount
Alcohol impregnates, and is heated to reflux 2~4 hours, collects extracting solution;The dregs of a decoction add 3~8 times of 50~90% ethyl alcohol of amount, it is heated to reflux 1~
3 hours, extracting solution is collected, merges secondary raffinate, is filtered to get root of Dahurain angelica extracting solution.
7. the skin mucosa disinfectant of wound healing according to claim 1-5, which is characterized in that the gold and silver
Flower extracting solution the preparation method comprises the following steps: weigh traditional Chinese medicine honeysuckle, select impurity, clean, be added 8~15 times of amount pure water, immersion 1~
It 2 hours, is heated to 80 DEG C~95 DEG C and extracts 1~3 time, 1~2 hour every time, filter, filtrate is spare;5~10 times of amounts are added in filter residue
60% ethyl alcohol is heated to 60 DEG C~80 DEG C and extracts 1~2 time, 1~2 hour every time, filters to get Flos Lonicerae extractive solution.
8. the skin mucosa disinfectant of wound healing according to claim 1-5, which is characterized in that the peppermint
Extracting solution the preparation method comprises the following steps: take peppermint medicinal material, carry out carbon dioxide supercritical extraction, be 8~10MPa, extraction in extracting pressure
Taking temperature is 45~60 DEG C, under conditions of carbon dioxide gas flow is 20~25L/min, extracts 1~1.5h, collects peppermint
Oil is dehydrated with anhydrous sodium sulfate, and filter paper filters to get peppermint extracting solution.
9. the skin mucosa disinfectant of wound healing according to claim 1-5, which is characterized in that the kuh-seng
Extracting solution the preparation method comprises the following steps: take Sophora flavescens, clean up, dry, slice, the water that 8~15 times of weight are added impregnates 2~
3h is heated to 85 DEG C~100 DEG C and extracts 1~2 time, 1~3 hour every time, filters, filtrate is spare;8~10 times of amounts are added in filter residue
60% ethyl alcohol heats 60 DEG C~80 DEG C, extracts 1~2 time, 1~2h, is filtered, merging filtrate is to get Radix Sophorae Flavescentis extractive liquid every time.
10. a kind of method for the skin mucosa disinfectant for preparing any one of claim 1-5 wound healing, it is characterised in that
It is made of following steps:
1) angelica root is weighed, impurity is selected, cleans, is ground into coarse powder, 5~10 times of 50~90% ethyl alcohol of amount is added to impregnate, heating
Extracting solution is collected in reflux 2~4 hours;The dregs of a decoction add 3~8 times of 50~90% ethyl alcohol of amount, are heated to reflux 1~3 hour, collect
Extracting solution, merges secondary raffinate, and filtering obtains root of Dahurain angelica extracting solution;
2) traditional Chinese medicine honeysuckle is weighed, impurity is selected, is cleaned, 8~15 times of amount pure water are added, impregnates 1~2 hour, is heated to 80
DEG C~95 DEG C extract 1~3 time, 1~2 hour every time, filtering, filtrate was spare;5~10 times of 60% ethyl alcohol of amount, heating is added in filter residue
It is extracted 1~2 time to 60 DEG C~80 DEG C, 1~2 hour every time, filtering obtained Flos Lonicerae extractive solution;
3) peppermint medicinal material is taken, carbon dioxide supercritical extraction is carried out, is 8~10MPa in extracting pressure, extraction temperature is 45~60
DEG C, under conditions of carbon dioxide gas flow is 20~25L/min, 1~1.5h is extracted, peppermint oil is collected, uses anhydrous sodium sulfate
Dehydration, filter paper filtering, obtains peppermint extracting solution;
4) Sophora flavescens are taken, are cleaned up, are dried, slice, the water that 8~15 times of weight are added impregnates 2~3h, be heated to 85 DEG C~
100 DEG C are extracted 1~2 time, 1~3 hour every time, are filtered, filtrate is spare;60% ethyl alcohol of 8~10 times of amounts, heating 60 is added in filter residue
DEG C~80 DEG C, it extracts 1~2 time, 1~2h, filtering, merging filtrate obtain Radix Sophorae Flavescentis extractive liquid every time;
5) step 1) to the extracting solution in 4) is taken to stir at low speed uniformly mixed, mixing speed is 600~900rpm;Mixing time is
5~10min obtains extraction mixed liquor A;
6) at 35 DEG C~50 DEG C, polyhexamethylene guanide (PHMG) is slowly added in low deuterium-oxide, constant temperature stirring, mixing speed is
650~900rpm, mixing time are 5~10min, obtain low deuterium-oxide solution B;
7) the extraction mixed liquor A of step 5) is slowly added to be mixed in the solution B of step 6), mixing speed 800
~1000rpm, time are 10~25min to get skin mucosa disinfectant.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201811370414.8A CN109172634A (en) | 2018-11-19 | 2018-11-19 | A kind of skin mucosa disinfectant and preparation method thereof for wound healing |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201811370414.8A CN109172634A (en) | 2018-11-19 | 2018-11-19 | A kind of skin mucosa disinfectant and preparation method thereof for wound healing |
Publications (1)
Publication Number | Publication Date |
---|---|
CN109172634A true CN109172634A (en) | 2019-01-11 |
Family
ID=64939649
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201811370414.8A Pending CN109172634A (en) | 2018-11-19 | 2018-11-19 | A kind of skin mucosa disinfectant and preparation method thereof for wound healing |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN109172634A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109966362A (en) * | 2019-05-21 | 2019-07-05 | 宁士莲 | A kind of general antibacterial liquid of men and women's nursing |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102397337A (en) * | 2010-09-15 | 2012-04-04 | 沃德国泰(北京)科技发展有限公司 | Traditional Chinese drug disinfectant and preparation method thereof |
CN103271985A (en) * | 2013-06-14 | 2013-09-04 | 柯美华 | Disinfectant for skin and mucosa |
-
2018
- 2018-11-19 CN CN201811370414.8A patent/CN109172634A/en active Pending
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102397337A (en) * | 2010-09-15 | 2012-04-04 | 沃德国泰(北京)科技发展有限公司 | Traditional Chinese drug disinfectant and preparation method thereof |
CN103271985A (en) * | 2013-06-14 | 2013-09-04 | 柯美华 | Disinfectant for skin and mucosa |
Non-Patent Citations (2)
Title |
---|
张亚茹,等: "低氘水对皮肤成纤维细胞及黑色素瘤细胞的影响", 《天然产物研究与开发》 * |
赵洁,等: "咽康片主要原料体外抑菌实验研究", 《中国医药科学》 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109966362A (en) * | 2019-05-21 | 2019-07-05 | 宁士莲 | A kind of general antibacterial liquid of men and women's nursing |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN103689010B (en) | Medicine for hog house disinfection | |
CN104920518A (en) | Traditional Chinese medicine disinfection spray and preparation method thereof | |
CN106538638A (en) | A kind of Chinese medicine antiseptic and preparation method thereof | |
CN113081928B (en) | Plant antibacterial gel and preparation method and application thereof | |
CN107028848A (en) | A kind of disinfection solution of disposable hand natural green | |
CN106214573A (en) | A kind of medical disposable hand saver skin disinfectant solution and preparation method thereof | |
CN104762136A (en) | Compound tea tree essential oil with sterilization and disinfection effects and preparation method of compound tea tree essential oil | |
CN110327259B (en) | Wet tissue for children | |
CN110327222B (en) | Purely natural plant essential oil sterilization and disinfection wet tissue and preparation method thereof | |
CN111744054B (en) | Antibacterial wound surface dressing capable of promoting healing and preparation method thereof | |
CN109172634A (en) | A kind of skin mucosa disinfectant and preparation method thereof for wound healing | |
CN111345325A (en) | Method for preparing disinfectant by using Chinese herbal medicine brewing technology | |
CN105708763A (en) | Artemisia argyi Levl extract-containing floral water and preparation method thereof | |
CN108434378A (en) | A kind of preparation method of the special pure Chinese herbal medicine anti-bacterial lotion of gynaecology | |
CN108902204A (en) | A kind of natural air freshener and preparation method thereof | |
CN111481567B (en) | Compound ozone oil and preparation method and application thereof | |
CN108653402A (en) | giant knotweed skin antibacterial liquid | |
CN106344453A (en) | Novel acne cream and preparation method thereof | |
CN105497460A (en) | Disinfectant for disinfecting skins | |
CN108498672A (en) | A kind of hospital's thimerosal and preparation method thereof | |
CN105560763A (en) | Traditional Chinese medicine composition skin disinfectant | |
CN111658586A (en) | Traditional Chinese medicine composition with mite and acne removing functions and preparation method and application thereof | |
CN111329899A (en) | Skin mucosa disinfectant and using method thereof | |
CN109999077A (en) | A kind of fragrance dehumidifying, detoxicating, killing bacteria composition and its preparation method and application | |
CN104435604A (en) | Preparation method of Chinese herbal medicine skin mucosa disinfectant |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190111 |
|
RJ01 | Rejection of invention patent application after publication |