CN109168690B - Tilia tomentosa hard wood cutting seedling method - Google Patents
Tilia tomentosa hard wood cutting seedling method Download PDFInfo
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- CN109168690B CN109168690B CN201811250572.XA CN201811250572A CN109168690B CN 109168690 B CN109168690 B CN 109168690B CN 201811250572 A CN201811250572 A CN 201811250572A CN 109168690 B CN109168690 B CN 109168690B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
Abstract
The invention provides a Tilia tomentosa hardwood cutting method, which comprises the following steps: (1) selection and preparation of cutting slips, (2) establishment of a seedbed and preparation of a cutting medium, (3) pretreatment of cutting, (4) cutting, and (5) management after cutting. The hard branch cutting method has the advantages that the cutting shoots can take roots quickly and much, meanwhile, overground parts have more spread leaves and are consistent in growth vigor, the cutting rooting rate and the cutting germination rate are more than 85%, the seedling transplanting survival rate is more than 90%, the labor for cutting and management is reduced, the cost is low, and good economic benefit and social benefit can be generated.
Description
Technical Field
The invention belongs to the technical field of plant propagation, and particularly relates to a Tilia tomentosa hardwood cutting seedling raising method.
Background
Tilia tomentosa (Tilia tomench), tiliaceae Tilia deciduous trees, excellent urban greening tree species and excellent protecting forest tree species, and the leaves, flowers, barks and woods of the Tilia tomentosa (Tilia tomentosa Moench) have higher economic values.
At present, the domestic researches on tilia mandshurica are few, only auspicious researches on introduction adaptability, propagation technology and economic value are found, and only the twig cutting technology is researched in the research on the propagation technology, and the hard branch cutting technology is not researched; in addition, the Tilia miqueliana undergoes the research on drought resistance physiological and ecological characteristics of Tilia miqueliana in the seedling stage. Tilia tomentosa seeds are mainly bred in production, but the Tilia tomentosa seeds have low plumpness and deep dormancy characteristics, and great obstacles are brought to the seedling breeding of Tilia tomentosa. The cutting propagation is convenient to operate and simple and easy to implement, so that the method becomes one of the main approaches for the asexual propagation of Tilia Miqueliana Maxim, but the Tilia plant belongs to the tree species which are difficult to root, the cutting propagation is not widely applied in production due to the limitation of the prior art, and particularly, the rooting of the Tilia Miqueliana Maxim by hard branch cutting is very difficult.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides the Tilia tomentosa hardwood cutting method, which can effectively improve the rooting rate of Tilia tomentosa hardwood cutting in a short period, simultaneously can reduce the labor for cutting and management, has low cost and can generate good economic benefit and social benefit.
The invention aims to provide a Tilia tomentosa hardwood cutting method.
The second purpose of the invention is to provide the application of the hardwood cutting method in the propagation expanding culture of tilia argentea.
In order to achieve the purpose, the invention relates to the following technical scheme:
in a first aspect of the present invention, there is provided a method for tilia mandshurica hardwood cutting, comprising:
s1, cutting selection and preparation: selecting a tillering strip at the base part of tilia amurensis as a cutting slip after pruning;
s2, seedbed establishment and cuttage matrix preparation: constructing a bed in a high bed, leveling the land, and then sequentially paving river sand, soil and perlite on a soil layer;
s3, pretreatment of cuttage: treating the cuttings in the step S1 by using potassium permanganate and IBA in sequence;
s4, cuttage: before cutting, a long tool is used for punching holes on a cutting bed, then the cutting slips are inserted into perlite, and the cutting slips and the matrix around the insertion holes are compacted.
Further, in the step S1, the cutting slips are silver tilia mandshurica base shoot strips with the age of 2-3 years and the thickness of more than 0.5cm, and the time is selected to be 3 months before axillary buds shoot, at the moment, the silver tilia mandshurica base shoot strips contain rich nutrition and are vigorous, so that the cutting survival rate is improved;
the specific method for trimming the cutting shoot comprises the following steps: the biological upper end of the cutting shoot is trimmed into a plain section, the biological lower end is trimmed into a bevel section, the lower cutting opening is 0.5-1cm away from the axillary bud, and the length of the trimmed cutting shoot is 10-12 cm;
further, in the step S2, the slotting machine is preferably built in a full-automatic greenhouse, the thickness ratio of the river sand, the soil and the perlite is 8-12 cm: 8-12 cm: 13-16 cm; further preferably 10 cm: 10 cm: 15 cm; researches show that by adopting the mode as a cutting medium, the good water permeability can be ensured, and the nutritional requirements of the cuttings after rooting can be met, so that the cutting survival rate is obviously improved;
furthermore, in step S2, the seedbed and the cutting medium are sterilized by 800 times of carbendazim solution and then used;
further, in step S3, the method of pre-cutting treatment specifically includes: the cutting in the step S1 is firstly sterilized by 0.5 percent potassium permanganate solution for 5min and then treated by 1000mg/L IBA solution for 10 min;
further, in the step S4, the cutting depth is controlled to be 6-8cm, and water is thoroughly poured after cutting is finished;
further, the Tilia tomentosa hardwood cutting method further comprises S5. management after cutting, and the specific method comprises the following steps:
s5.1, cooling and moisturizing, and controlling the temperature to be 25 +/-2 ℃;
s5.2, alternately spraying leaf fertilizer and rooting powder solution until the cutting shoots root.
Under the control of the environmental conditions, the cutting shoots form callus after about 15 days of cutting, a large amount of adventitious roots are generated about 30 days, the rooting type of Tilia mandshurica hard wood cutting is mainly callus rooting, and only a small amount of bark is rooted.
In a second aspect of the invention, the application of the hardwood cutting method in the propagation expanding culture of tilia argentea is provided.
The invention has the beneficial technical effects that:
(1) the current-year-growing tiller strips on the 2-3-year-old mother trees are used as cutting slips, so that firstly, the nutrient substances of the cutting slips can meet the rooting requirement, and secondly, the vigorous meristematic capacity can be maintained, and the rooting is facilitated;
(2) the mode of one layer of river sand, one layer of loess and one layer of perlite is used as a cutting substrate, so that good water permeability can be ensured, and the nutritional requirements after the cutting shoots take root can be met, thereby promoting the Tilia tomentose to take root quickly and improving the survival rate of the Tilia tomentose;
in conclusion, the cutting method provided by the invention has the advantages that the cutting shoots can root quickly and much, meanwhile, the overground part has more spread leaves and is consistent in growth vigor, the cutting rooting rate and the cutting germination rate are more than 85%, the seedling transplanting survival rate is more than 90%, the labor for cutting and management is reduced, the cost is low, and good economic benefits and social benefits can be generated.
Drawings
FIG. 1 is a diagram showing rooting conditions of Tilia mandshurica hard branch cuttings in example 1.
Detailed Description
It should be noted that the following detailed description is exemplary and is intended to provide further explanation of the disclosure. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.
It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of example embodiments according to the present application. As used herein, the singular forms "a", "an" and "the" are intended to include the plural forms as well, and it should be understood that when the terms "comprises" and/or "comprising" are used in this specification, they specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof, unless the context clearly indicates otherwise.
As mentioned above, Tilia plants belong to species of trees difficult to root, and are limited by the prior art, the cutting propagation is not widely applied in production, and particularly, the rooting of hard wood cutting is extremely difficult.
In view of the above, in an exemplary embodiment of the present invention, there is provided a method for raising seedlings of tilia mandshurica by hardwood cutting, comprising the steps of: (1) selecting and preparing cutting slips, (2) establishing a seedbed and preparing a cutting medium, (3) pretreating cutting, (4) cutting, and (5) managing after cutting;
in another embodiment of the present invention, the cutting selection and preparation method in step (1) is: selecting robust and disease-and-insect-free base shoot strips with the thickness of more than 0.5cm and the tree age of 2-3 years as cutting slips before axillary buds sprout in the middle ten days of 3 months, cutting the cutting slips at the upper opening, performing flat shearing on the cutting slips, performing oblique shearing on the lower opening, wherein the lower opening is 0.5-1cm away from the axillary buds, and the average length is 10-12 cm; bundling 20 pieces in a group;
in another embodiment of the present invention, the method for establishing the seedbed and preparing the cutting medium in step (2) comprises: the method comprises the following steps of (1) building a slotting bed in a full-automatic greenhouse, removing weeds by adopting a high-ridge bed building, leveling the land, paving river sand with the thickness of 10cm on a soil layer, paving soil with the thickness of 10m on the river sand, paving perlite with the thickness of 15cm on the soil, and sterilizing 800 times of carbendazim solution for later use;
in another embodiment of the present invention, the method for treating before cutting in step (3) comprises: sterilizing with 0.5% potassium permanganate solution for 5min before cutting, and treating with 1000mg/L IBA solution for 10 min;
in another embodiment of the present invention, the cutting method in step (4) comprises: punching a hole on a cutting bed by using a strip tool with a diameter slightly larger than that of the cutting slip before cutting, then inserting the cutting slip into perlite along the inclined direction of a lower incision, wherein the cutting depth is 6-8cm, compacting the cutting slip and a matrix around an insertion hole, and pouring water thoroughly after cutting;
in another embodiment of the present invention, the method for managing after-insertion in step (5) includes: after the cuttage is finished, a temperature and humidity recorder is installed in the greenhouse, and the temperature of the greenhouse is controlled to be about 25 ℃; cooling and moisturizing by using a full-automatic intermittent spraying device, and automatically spraying for 10s every 1 h; and alternately spraying foliar fertilizer and rooting powder solution within each week until the cutting shoots take roots, so as to ensure that the cutting shoots obtain sufficient nutrition and smoothly take roots after leaf unfolding.
The invention is further illustrated by the following examples, which are not to be construed as limiting the invention thereto. It should be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention. The test methods in the following examples, which are not specified under specific conditions, are generally carried out under conventional conditions.
Example 1
A Tilia tomentosa hardwood cutting seedling raising method comprises the following specific steps:
(1) selection and preparation of cutting slips: before axillary buds sprout in the middle ten days of 3 months, selecting robust and disease-free base shoot strips with the thickness of more than 0.5cm and the tree age of 2-3 years as cutting slips, cutting the cutting slips flatly, cutting the lower cut obliquely, wherein the lower cut is 0.5-1cm away from the axillary buds, and the average length is 10-12 cm; bundling 20 pieces in a group;
(2) establishing a cutting bed and preparing a cutting medium: the method comprises the following steps of (1) building a slotting bed in a full-automatic greenhouse, removing weeds by adopting a high-ridge bed building, leveling the land, paving river sand with the thickness of 10cm on a soil layer, paving soil with the thickness of 10m on the river sand, paving perlite with the thickness of 15cm on the soil, and sterilizing 800 times of carbendazim solution for later use;
(3) pretreatment in cuttage: sterilizing with 0.5% potassium permanganate solution for 5min before cutting, and treating with 1000mg/L IBA solution for 10 min;
(4) cuttage: punching a hole on a cutting bed by using a strip tool with a diameter slightly larger than that of the cutting slip before cutting, then inserting the cutting slip into perlite along the inclined direction of a lower incision, wherein the cutting depth is 6-8cm, compacting the cutting slip and a matrix around an insertion hole, and pouring water thoroughly after cutting;
(5) inserting the rear part: after cutting, installing a temperature and humidity recorder in the greenhouse, adjusting the air temperature at any time according to the ground temperature, keeping the air temperature 2-3 ℃ lower than the ground temperature all the time before the cutting shoots root, and controlling the temperature of the greenhouse to be about 25 ℃; cooling and moisturizing by using a full-automatic intermittent spraying device, and automatically spraying for 10s every 1 h; and alternately spraying foliar fertilizer and rooting powder solution within each week until the cutting shoots take roots, so as to ensure that the cutting shoots obtain sufficient nutrition and smoothly take roots after leaf unfolding.
About 15 days after cuttage, the cuttings form callus, a large number of adventitious roots are generated about 30 days, the rooting type of Tilia tomentosa hard wood cuttage is mainly the callus rooting, only a small number of bark roots are seen, the root system is developed after 45 days, meanwhile, overground parts are more in leaf spread and more consistent in growth vigor, the cuttage germination rate (the number of cuttings for germination/the total number of cuttings for cuttage) is 93%, the rooting rate of the cuttage seedlings (the number of cuttings for germination and rooting/the total number of cuttings for cuttage) is 89%, and the transplanting survival rate (the number of surviving seedlings/the total number of seedlings) is 92%.
Example 2
A Tilia tomentosa hardwood cutting seedling raising method comprises the following specific steps:
(1) selection and preparation of cutting slips: before axillary buds sprout in the middle ten days of 3 months, selecting robust and disease-free base shoot strips with the thickness of more than 0.5cm and the tree age of 2-3 years as cutting slips, cutting the cutting slips flatly, cutting the lower cut obliquely, wherein the lower cut is 0.5-1cm away from the axillary buds, and the average length is 10-12 cm; bundling 20 pieces in a group;
(2) establishing a cutting bed and preparing a cutting medium: the method comprises the following steps of (1) building a slotting bed in a full-automatic greenhouse, adopting a high-ridge bed building, removing weeds, leveling the land, paving perlite with the thickness of 10cm on a soil layer, paving soil with the thickness of 10m on the perlite, paving river sand with the thickness of 15cm on the soil, and disinfecting with 800 times of carbendazim solution for later use;
(3) pretreatment in cuttage: sterilizing with 0.5% potassium permanganate solution for 5min before cutting, and treating with 1000mg/L IBA solution for 10 min;
(4) cuttage: punching a hole on a cutting bed by using a strip tool with a diameter slightly larger than that of the cutting slip before cutting, then inserting the cutting slip into river sand along the inclined direction of a lower incision, wherein the cutting depth is 6-8cm, compacting the cutting slip and a matrix around an insertion hole, and pouring water thoroughly after cutting;
(5) inserting the rear part: after the cuttage is finished, a temperature and humidity recorder is installed in the greenhouse, and the temperature of the greenhouse is controlled to be about 25 ℃; cooling and moisturizing by using a full-automatic intermittent spraying device, and automatically spraying for 10s every 1 h; and alternately spraying foliar fertilizer and rooting powder solution within each week until the cutting shoots take roots, so as to ensure that the cutting shoots obtain sufficient nutrition and smoothly take roots after leaf unfolding.
After about 25 days of cutting, the cutting shoots form callus, more adventitious roots are generated in about 45 days, the rooting type of Tilia tomentosa hard wood cutting is mainly the callus rooting, the cutting germination rate (the number of cuttings for germination/the total number of cuttings for cutting) is 62%, the rooting rate of cutting seedlings (the number of cuttings for germination and rooting/the total number of cuttings for cutting) is 58%, and the transplanting survival rate (the number of surviving seedlings/the total number of seedlings) is 76%.
Example 3
A Tilia tomentosa hardwood cutting seedling raising method comprises the following specific steps:
(1) selection and preparation of cutting slips: before axillary buds sprout in the middle ten days of 3 months, selecting robust and disease-free base shoot strips with the thickness of more than 0.5cm and the tree age of 2-3 years as cutting slips, cutting the cutting slips flatly, cutting the lower cut obliquely, wherein the lower cut is 0.5-1cm away from the axillary buds, and the average length is 10-12 cm; bundling 20 pieces in a group;
(2) establishing a cutting bed and preparing a cutting medium: the method comprises the following steps of (1) building a slotting bed in a full-automatic greenhouse, adopting a high-ridge bed building to remove weeds, leveling the land, paving a mixture of river sand, soil and perlite on a soil layer, wherein the volume ratio of the river sand to the soil to the perlite is 1:1:1.5, and controlling the thickness of the mixture to be 35 cm; sterilizing 800 times of carbendazim solution for later use;
(3) pretreatment in cuttage: sterilizing with 0.5% potassium permanganate solution for 5min before cutting, and treating with 1000mg/LIBA solution for 10 min;
(4) cuttage: punching a hole on a cutting bed by using a strip tool with a diameter slightly larger than that of the cutting slip before cutting, then inserting the cutting slip into the mixed matrix along the inclined direction of a lower incision, wherein the cutting depth is 6-8cm, compacting the cutting slip and the matrix around the insertion hole, and watering thoroughly after cutting;
(5) inserting the rear part: after the cuttage is finished, a temperature and humidity recorder is installed in the greenhouse, and the temperature of the greenhouse is controlled to be about 25 ℃; cooling and moisturizing by using a full-automatic intermittent spraying device, and automatically spraying for 10s every 1 h; and alternately spraying foliar fertilizer and rooting powder solution within each week until the cutting shoots take roots, so as to ensure that the cutting shoots obtain sufficient nutrition and smoothly take roots after leaf unfolding.
About 20 days after cuttage, the cutting shoots form callus, more adventitious roots are generated about 40 days, the rooting type of Tilia tomentosa hard wood cuttage is mainly the callus rooting, the cuttage germination rate (the number of cuttings for germination/the total number of cuttings for cuttage) is 75%, the rooting rate of cutting seedlings (the number of cuttings for germination and rooting/the total number of cuttings for cuttage) is 70%, and the transplanting survival rate (the number of surviving seedlings/the total number of seedlings) is 82%.
Example 4
A Tilia tomentosa hardwood cutting seedling raising method comprises the following specific steps:
(1) selection and preparation of cutting slips: before axillary buds sprout in the middle ten days of 3 months, selecting robust and disease-free base shoot strips with the thickness of more than 0.5cm and the tree age of 2-3 years as cutting slips, cutting the cutting slips flatly, cutting the lower cut obliquely, wherein the lower cut is 0.5-1cm away from the axillary buds, and the average length is 10-12 cm; bundling 20 pieces in a group;
(2) establishing a cutting bed and preparing a cutting medium: the method comprises the following steps of (1) building a slotting bed in a full-automatic greenhouse, removing weeds by adopting a high-ridge bed building, leveling the land, paving river sand with the thickness of 10cm on a soil layer, paving soil with the thickness of 10m on the river sand, paving perlite with the thickness of 15cm on the soil, and sterilizing 800 times of carbendazim solution for later use;
(3) pretreatment in cuttage: sterilizing with 0.5% potassium permanganate solution for 5min before cutting, and treating with 1000mg/L NAA solution for 10 min;
(4) cuttage: punching a hole on a cutting bed by using a strip tool with a diameter slightly larger than that of the cutting slip before cutting, then inserting the cutting slip into perlite along the inclined direction of a lower incision, wherein the cutting depth is 6-8cm, compacting the cutting slip and a matrix around an insertion hole, and pouring water thoroughly after cutting;
(5) inserting the rear part: after the cuttage is finished, a temperature and humidity recorder is installed in the greenhouse, and the temperature of the greenhouse is controlled to be about 25 ℃; cooling and moisturizing by using a full-automatic intermittent spraying device, and automatically spraying for 10s every 1 h; and alternately spraying foliar fertilizer and rooting powder solution within each week until the cutting shoots take roots, so as to ensure that the cutting shoots obtain sufficient nutrition and smoothly take roots after leaf unfolding.
About 18 days after cuttage, the cuttings form callus, a large amount of adventitious roots are generated about 35 days, the rooting type of Tilia tomentosa hard wood cuttage is mainly the callus rooting, only a small amount of bark roots are seen, the root system is developed after 50 days, overground parts have less spread leaves, the cuttage germination rate (the number of cuttings for germination/the total number of cuttings for cuttage) is 80%, the rooting rate of the cutting seedlings (the number of cuttings for germination and rooting/the total number of cuttings for cuttage) is 72%, and the transplanting survival rate (the number of surviving seedlings/the total number of seedlings) is 78%.
It should be noted that the above examples are only used to illustrate the technical solutions of the present invention and not to limit them. Although the present invention has been described in detail with reference to the examples given, those skilled in the art can modify the technical solution of the present invention as needed or equivalent substitutions without departing from the spirit and scope of the technical solution of the present invention.
Claims (6)
1. The application of the Tilia tomentosa hardwood cutting method in improving the germination rate, rooting rate and transplanting survival rate of Tilia tomentosa propagation culture is characterized by comprising the following steps of:
s1, cutting selection and preparation: selecting a tillering strip at the base part of tilia amurensis as a cutting slip after pruning; selecting silver tilia mandshurica base sprout strips with the tree age of 2-3 years and the thickness of more than 0.5cm for the cutting slips, wherein the time is selected to be before axillary sprout in 3 middle ten days; the biological upper end of the cutting shoot is trimmed into a plain section, the biological lower end is trimmed into a bevel section, the lower cutting opening is 0.5-1cm away from the axillary bud, and the length of the trimmed cutting shoot is 10-12 cm;
s2, seedbed establishment and cuttage matrix preparation: constructing a bed in a high bed, leveling the land, and then sequentially paving river sand, soil and perlite on a soil layer; the thickness ratio of the river sand to the soil to the perlite is 8-12 cm: 8-12 cm: 13-16 cm;
s3, pretreatment of cuttage: sterilizing the cutting in the step S1 with 0.5% potassium permanganate solution for 5min, and then treating with 1000mg/L IBA solution for 10 min;
s4, cuttage: before cutting, a long tool is used for punching holes on a cutting bed, then the cutting slips are inserted into perlite, and the cutting slips and the matrix around the insertion holes are compacted.
2. The application of the tilia amurensis hardwood cutting method in improving germination rate, rooting rate and transplanting survival rate in tilia amurensis propagation culture according to claim 1, wherein in S2, a cutting bed is built in a fully automatic greenhouse.
3. The application of the tilia argentea hardwood cutting method in improving germination rate, rooting rate and transplanting survival rate of tilia argentea propagation culture according to claim 1, wherein the thickness ratio of river sand, soil and perlite is 10 cm: 10 cm: 15 cm.
4. The application of the tilia argentea hardwood cutting method in improving germination rate, rooting rate and transplanting survival rate in propagation culture of tilia argentea according to claim 1, wherein in S2, the seedbed and the cutting substrate are sterilized by 800 times of carbendazim solution and then used.
5. The application of the tilia argentea hardwood cutting method in improving germination rate, rooting rate and transplanting survival rate in propagation culture of tilia argentea according to claim 1, wherein in S4, the cutting depth is controlled to be 6-8cm, and water is permeated after cutting.
6. The application of the tilia amurensis hardwood cutting method in improving germination rate, rooting rate and transplanting survival rate of tilia amurensis propagation culture according to claim 1, wherein the tilia amurensis hardwood cutting method further comprises S5. management after cutting, and the specific method is as follows:
s5.1, cooling and moisturizing, and controlling the temperature to be 25 +/-2 ℃;
s5.2, alternately spraying leaf fertilizer and rooting powder solution until the cutting shoots root.
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| CN103597976A (en) * | 2013-10-18 | 2014-02-26 | 赵刚 | Seedling cultivation and propagation method for tilia amurensis |
| CN105009848A (en) * | 2014-04-16 | 2015-11-04 | 胡月 | Kiwi berry green-wood cutting method |
| CN104620900B (en) * | 2015-03-09 | 2017-06-27 | 山东省潍坊市农业科学院 | A kind of cuttage and seedling culture seedbed and preparation method thereof |
| CN106069506A (en) * | 2016-06-23 | 2016-11-09 | 安徽鸿基农业科技发展有限公司 | A kind of cuttage technique of tilia |
| CN206462088U (en) * | 2017-02-14 | 2017-09-05 | 山西省农业科学院果树研究所 | A kind of seedbed for cherry root stock cutting |
| CN106922507A (en) * | 2017-03-07 | 2017-07-07 | 江苏省林业科学研究院 | A kind of tilia miqueliana root cutting growth method |
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