CN109071589A - 5′-硫代磷酸酯mRNA 5′-端(帽)类似物、包含所述类似物的mRNA、其获得方法和用途 - Google Patents

5′-硫代磷酸酯mRNA 5′-端(帽)类似物、包含所述类似物的mRNA、其获得方法和用途 Download PDF

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Publication number
CN109071589A
CN109071589A CN201780013350.3A CN201780013350A CN109071589A CN 109071589 A CN109071589 A CN 109071589A CN 201780013350 A CN201780013350 A CN 201780013350A CN 109071589 A CN109071589 A CN 109071589A
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mrna
independently selected
alkyl
formula
cap
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CN201780013350.3A
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Chinese (zh)
Inventor
J.杰米勒提
K.法克-达布罗瓦斯卡
B.沃杰特泽克
M.巴兰诺瓦斯基
A.诺维卡
J.科瓦勒斯卡
P.斯克尔斯基
M.瓦尔敏斯基
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Uniwersytet Warszawski
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Uniwersytet Warszawski
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Publication of CN109071589A publication Critical patent/CN109071589A/zh
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P21/00—Drugs for disorders of the muscular or neuromuscular system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P21/00—Drugs for disorders of the muscular or neuromuscular system
    • A61P21/02—Muscle relaxants, e.g. for tetanus or cramps
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/02—Phosphorylation
    • C07H1/04—Introducing polyphosphoric acid radicals

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Pharmacology & Pharmacy (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Neurology (AREA)
  • Physical Education & Sports Medicine (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Biomedical Technology (AREA)
  • Neurosurgery (AREA)
  • Pain & Pain Management (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Saccharide Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201780013350.3A 2016-01-29 2017-01-27 5′-硫代磷酸酯mRNA 5′-端(帽)类似物、包含所述类似物的mRNA、其获得方法和用途 Pending CN109071589A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
PLPL415967 2016-01-29
PL415967A PL415967A1 (pl) 2016-01-29 2016-01-29 5'-tiofosforanowe analogi końca 5' mRNA (kapu), sposób ich otrzymywania i zastosowanie
PCT/IB2017/050447 WO2017130151A1 (en) 2016-01-29 2017-01-27 5'-phosphorothiolate mrna 5'-end (cap) analogs, mrna comprising the same, method of obtaining and uses thereof

Publications (1)

Publication Number Publication Date
CN109071589A true CN109071589A (zh) 2018-12-21

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CN201780013350.3A Pending CN109071589A (zh) 2016-01-29 2017-01-27 5′-硫代磷酸酯mRNA 5′-端(帽)类似物、包含所述类似物的mRNA、其获得方法和用途

Country Status (11)

Country Link
US (1) US11066436B2 (enExample)
EP (1) EP3408276B1 (enExample)
JP (1) JP7144050B2 (enExample)
KR (1) KR102742219B1 (enExample)
CN (1) CN109071589A (enExample)
AU (1) AU2017211693B2 (enExample)
CA (1) CA3011663C (enExample)
EA (1) EA038834B1 (enExample)
ES (1) ES2879802T3 (enExample)
PL (2) PL415967A1 (enExample)
WO (1) WO2017130151A1 (enExample)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111944864A (zh) * 2020-08-20 2020-11-17 深圳市瑞吉生物科技有限公司 一种新型Cap2结构5′帽子类似物及其制备方法
CN116143855A (zh) * 2022-11-08 2023-05-23 江苏申基生物科技有限公司 一种乙烯基膦酸修饰的mRNA帽类似物及其制备方法和应用
WO2023213237A1 (zh) * 2022-05-05 2023-11-09 江苏申基生物科技有限公司 一种含开环核苷结构的起始加帽寡核苷酸引物

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE112020002327T5 (de) * 2019-05-10 2022-07-14 New England Biolabs, Inc. Chemisches verkappen für matrizenwechsel
CN110907358B (zh) * 2019-12-09 2022-08-30 福建师范大学 一种光微流微泡腔铅离子传感器的实现装置及方法
PL432884A1 (pl) * 2020-02-12 2021-08-16 Uniwersytet Warszawski Nowe analogi kapu końca 5' mRNA, cząsteczka RNA je zawierająca, ich zastosowania i sposób syntezy cząsteczki RNA i peptydu
CN116368226A (zh) 2020-09-04 2023-06-30 维乎医疗有限公司 用于加帽rna的组合物和方法
WO2023167880A2 (en) * 2022-03-01 2023-09-07 Verve Therapeutics, Inc. Compositions and methods for capping rnas
EP4464713A1 (en) 2023-05-18 2024-11-20 Siec Badawcza Lukasiewicz - PORT Polski Osrodek Rozwoju Technologii Circular rna analogs, preparation and application thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009149253A2 (en) * 2008-06-06 2009-12-10 Uniwersytet Warszawski Mrna cap analogs
CN101855231A (zh) * 2007-06-19 2010-10-06 路易斯安那州州立大学及农业机械学院管理委员会 信使rna帽的抗-反向硫代磷酸类似物的合成和用途

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008546424A (ja) 2005-06-28 2008-12-25 アジェンコート パーソナル ジェノミクス コーポレイション 修飾ポリヌクレオチドを作製する方法および配列決定する方法
EP2281579A1 (en) * 2009-08-05 2011-02-09 BioNTech AG Vaccine composition comprising 5'-Cap modified RNA
EP2970351B1 (en) * 2013-03-14 2017-09-13 Translate Bio, Inc. Ribonucleic acids with 4'-thio-modified nucleotides and related methods
HRP20200250T1 (hr) 2013-08-08 2020-05-29 The Scripps Research Institute Metoda site-specifično enzimsko obilježavanje nukleinskih kiselina in vitro ugradnjom neprirodnih nukleotida

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101855231A (zh) * 2007-06-19 2010-10-06 路易斯安那州州立大学及农业机械学院管理委员会 信使rna帽的抗-反向硫代磷酸类似物的合成和用途
SI2167523T1 (sl) * 2007-06-19 2014-09-30 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Sinteza in uporaba anti-reverznih fosforotioatnih analogov kape obveščevalne RNA
CN104072561A (zh) * 2007-06-19 2014-10-01 路易斯安那州州立大学及农业机械学院管理委员会 信使rna帽的抗-反向硫代磷酸类似物的合成和用途
WO2009149253A2 (en) * 2008-06-06 2009-12-10 Uniwersytet Warszawski Mrna cap analogs

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
KIM, IH ET AL.: "Two-step synthesis of 5 "-deoxy-5 "-thioguanosine-5 "-monophosphorothioate and its incorporation efficiency into 5 "-terminus of RNA for preparation of thiol-functionalized RNA", 《TETRAHEDRON LETTERS》 *
KOWALSKA, J ET AL.: "Synthesis and characterization of mRNA cap analogs containing phosphorothioate substitutions that bind tightly to eIF4E and are resistant to the decapping pyrophosphatase DcpS", 《RNA》 *
SCHMITZ, K ET AL.: "Modified GSMP Synthesis Greatly Improves the Disulfide Crosslink of T7Run-Off siRNAs with Cell Penetrating Peptides", 《SYNLETT》 *
THEODORA W.GREENE等: "《有机合成中的保护基》", 31 October 2004, 华东理工大学出版社 *
WYPIJEWSKA DEL NOGAL, A ET AL.: "Analysis of decapping scavenger cap complex using modified cap analogs reveals molecular determinants for efficient cap binding", 《FEBS JOURNAL》 *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111944864A (zh) * 2020-08-20 2020-11-17 深圳市瑞吉生物科技有限公司 一种新型Cap2结构5′帽子类似物及其制备方法
CN111944864B (zh) * 2020-08-20 2021-06-22 深圳市瑞吉生物科技有限公司 一种Cap2结构5＇帽子类似物及其制备方法
CN113106135A (zh) * 2020-08-20 2021-07-13 深圳市瑞吉生物科技有限公司 一种Cap2结构5′帽子类似物及其制备方法和应用
WO2023213237A1 (zh) * 2022-05-05 2023-11-09 江苏申基生物科技有限公司 一种含开环核苷结构的起始加帽寡核苷酸引物
CN116143855A (zh) * 2022-11-08 2023-05-23 江苏申基生物科技有限公司 一种乙烯基膦酸修饰的mRNA帽类似物及其制备方法和应用
CN116143855B (zh) * 2022-11-08 2023-11-28 江苏申基生物科技有限公司 一种乙烯基膦酸修饰的mRNA帽类似物及其制备方法和应用

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Publication number Publication date
AU2017211693B2 (en) 2021-09-09
US20190300563A1 (en) 2019-10-03
CA3011663A1 (en) 2017-08-03
JP2019504837A (ja) 2019-02-21
EP3408276B1 (en) 2021-04-07
AU2017211693A1 (en) 2018-08-09
ES2879802T3 (es) 2021-11-23
JP7144050B2 (ja) 2022-09-29
US11066436B2 (en) 2021-07-20
CA3011663C (en) 2023-10-31
EA038834B1 (ru) 2021-10-26
KR102742219B1 (ko) 2024-12-13
EA201891429A1 (ru) 2019-02-28
EP3408276A1 (en) 2018-12-05
PL415967A1 (pl) 2017-07-31
WO2017130151A1 (en) 2017-08-03
PL3408276T3 (pl) 2021-10-25
KR20180100595A (ko) 2018-09-11

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