CN109001329A - 干血片中25-羟基维生素d的高效液相色谱串联质谱检测方法 - Google Patents
干血片中25-羟基维生素d的高效液相色谱串联质谱检测方法 Download PDFInfo
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- CN109001329A CN109001329A CN201810951503.5A CN201810951503A CN109001329A CN 109001329 A CN109001329 A CN 109001329A CN 201810951503 A CN201810951503 A CN 201810951503A CN 109001329 A CN109001329 A CN 109001329A
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Classifications
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N2030/042—Standards
- G01N2030/045—Standards internal
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
- G01N2030/062—Preparation extracting sample from raw material
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- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
- Investigating Or Analysing Biological Materials (AREA)
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CN201810951503.5A CN109001329B (zh) | 2018-08-21 | 2018-08-21 | 干血片中25-羟基维生素d的高效液相色谱串联质谱检测方法 |
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Cited By (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110174476A (zh) * | 2019-06-10 | 2019-08-27 | 合肥谱佳医学检验实验室有限公司 | 一种干血片中多种脂溶性维生素的液相色谱串联质谱的检测方法 |
US10656059B2 (en) | 2018-03-07 | 2020-05-19 | Alcala Pharmaceutical, Inc. | Method for qualitative and quantitative multiplexing of drug analytes from biological samples |
CN111474255A (zh) * | 2020-04-16 | 2020-07-31 | 上海中科新生命生物科技有限公司 | 一种基于干血片样本检测25羟基维生素d的方法 |
CN111830146A (zh) * | 2020-05-27 | 2020-10-27 | 江苏豪思睦可生物科技有限公司 | 一种干血片中25-羟基维d的lc-ms/ms高通量检测方法及试剂盒 |
CN111855832A (zh) * | 2020-05-27 | 2020-10-30 | 江苏豪思睦可生物科技有限公司 | 一种维d及其代谢物的质谱检测用校准和质控品的制备方法 |
CN111983071A (zh) * | 2020-08-13 | 2020-11-24 | 上海交通大学医学院附属上海儿童医学中心 | 一种末梢微量血维生素d质谱检测方法 |
CN113640428A (zh) * | 2021-08-24 | 2021-11-12 | 青岛惠安康生物工程有限公司 | 干血片中25-羟基维生素d的液相色谱串联质谱检测方法及试剂盒 |
CN115656381A (zh) * | 2022-11-10 | 2023-01-31 | 成都新基因格医学检验所有限公司 | 一种小体积样本脂溶性维生素d的定量检测方法 |
CN116381113A (zh) * | 2023-04-12 | 2023-07-04 | 张义 | 干血片中同时检测五种脂溶性维生素的液相色谱串联质谱检测方法 |
CN118090878A (zh) * | 2024-04-26 | 2024-05-28 | 中国科学技术大学 | 检测生物样本中脂溶性维生素的方法 |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9188596B2 (en) * | 2010-01-25 | 2015-11-17 | Dh Technologies Development Pte. Ltd | Quantitative analysis of vitamin D3, vitamin D2, and metabolites thereof |
CN105651901A (zh) * | 2016-04-11 | 2016-06-08 | 北京洛奇临床检验所股份有限公司 | 干血片样品中25羟基维生素d的液相色谱串联质谱检测方法及试剂盒 |
CN106770802A (zh) * | 2017-02-23 | 2017-05-31 | 广州市丰华生物工程有限公司 | 一种同时检测干血滤纸片中多种维生素的方法和试剂盒 |
CN106908554A (zh) * | 2017-03-24 | 2017-06-30 | 北京爱普益医学检验中心有限公司 | 一种干血斑中维生素d含量的检测方法 |
-
2018
- 2018-08-21 CN CN201810951503.5A patent/CN109001329B/zh active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9188596B2 (en) * | 2010-01-25 | 2015-11-17 | Dh Technologies Development Pte. Ltd | Quantitative analysis of vitamin D3, vitamin D2, and metabolites thereof |
CN105651901A (zh) * | 2016-04-11 | 2016-06-08 | 北京洛奇临床检验所股份有限公司 | 干血片样品中25羟基维生素d的液相色谱串联质谱检测方法及试剂盒 |
CN106770802A (zh) * | 2017-02-23 | 2017-05-31 | 广州市丰华生物工程有限公司 | 一种同时检测干血滤纸片中多种维生素的方法和试剂盒 |
CN106908554A (zh) * | 2017-03-24 | 2017-06-30 | 北京爱普益医学检验中心有限公司 | 一种干血斑中维生素d含量的检测方法 |
Non-Patent Citations (3)
Title |
---|
DARRYL EYLES 等: "A sensitive LC/MS/MS assay of 25OH vitamin D3 and 25OH vitamin D2 in dried blood spots", 《CLINICA CHIMICA ACTA》 * |
徐凤仙 等: "液相色谱串联质谱法测定血清中25-羟基维生素D2及25-羟基维生素D3", 《检验医学》 * |
韩吉春 等: "液相色谱-串联质谱法检测干血点样本中25-羟基维生素D2和25-羟基维生素D3", 《分析化学》 * |
Cited By (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US10656059B2 (en) | 2018-03-07 | 2020-05-19 | Alcala Pharmaceutical, Inc. | Method for qualitative and quantitative multiplexing of drug analytes from biological samples |
US11054349B2 (en) | 2018-03-07 | 2021-07-06 | Alcala Pharmaceutical, Inc. | Method for preparation of dried blood sample for multiplexing of analytes |
CN110174476A (zh) * | 2019-06-10 | 2019-08-27 | 合肥谱佳医学检验实验室有限公司 | 一种干血片中多种脂溶性维生素的液相色谱串联质谱的检测方法 |
CN111474255A (zh) * | 2020-04-16 | 2020-07-31 | 上海中科新生命生物科技有限公司 | 一种基于干血片样本检测25羟基维生素d的方法 |
CN111830146A (zh) * | 2020-05-27 | 2020-10-27 | 江苏豪思睦可生物科技有限公司 | 一种干血片中25-羟基维d的lc-ms/ms高通量检测方法及试剂盒 |
CN111855832A (zh) * | 2020-05-27 | 2020-10-30 | 江苏豪思睦可生物科技有限公司 | 一种维d及其代谢物的质谱检测用校准和质控品的制备方法 |
CN111830146B (zh) * | 2020-05-27 | 2023-09-01 | 江苏豪思睦可生物科技有限公司 | 一种干血片中25-羟基维d的lc-ms/ms高通量检测方法及试剂盒 |
CN111983071A (zh) * | 2020-08-13 | 2020-11-24 | 上海交通大学医学院附属上海儿童医学中心 | 一种末梢微量血维生素d质谱检测方法 |
CN113640428A (zh) * | 2021-08-24 | 2021-11-12 | 青岛惠安康生物工程有限公司 | 干血片中25-羟基维生素d的液相色谱串联质谱检测方法及试剂盒 |
CN115656381A (zh) * | 2022-11-10 | 2023-01-31 | 成都新基因格医学检验所有限公司 | 一种小体积样本脂溶性维生素d的定量检测方法 |
CN116381113A (zh) * | 2023-04-12 | 2023-07-04 | 张义 | 干血片中同时检测五种脂溶性维生素的液相色谱串联质谱检测方法 |
CN118090878A (zh) * | 2024-04-26 | 2024-05-28 | 中国科学技术大学 | 检测生物样本中脂溶性维生素的方法 |
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